Rh4AG436500

4Fe-4S single cluster domain of Ferredoxin I

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014546
Physical Location & Seq
Reverse (-)
72959197 .. 72961749
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG436500.1

Sequence Viewer

Length: 1176 bp
ATGCAGGAGCACTCGAAAATTGCAGAGCTCCGAGGTTACTCAGGGAAGCCTCTTTACTCGTCATGGTTTGGTTCAGAAACCGAAGAACGAGCAGTTTTCGTGGATGAAGTCAAGTGTATTGGTTGCTTGAAATGTGCGTTATGTGCTGAAAAAACTTTTGCAATTGAATCCGTGTATGGAAGAGCCAGGGTTGTCGGACAGTGGGCTGACCCTGAACACAAAATACAGCAGGCTATACAGGCTTGTCCTGTTGATTGCATCTCGGTTGTGGAGAGGTCGGACCTAGCAGCTCTGGAATTTCTAATGTCCAAGCAGCCACGTGGCAATGTAAGAATAGGAATGGGGAATTCAGCCGGTATACGAGTCTCCAACATCTTTGTTGAGGTGAAAAAATTCAAGACAAGATTCCAAGATGCAAAACAGAAAGCTTCTGCACAGAGTTCTAATGAGACAGACTTACAAAGAGAAGCAAGAGTATCAGCAATTCAAGCCATCAGATCCATCTCCAACTGGTTGTATTGGACACCCTTTGCAAGTGCAGGTGAAACCAGTTCCAGAAGTACAACAAAGCCTCTCCAACATTTAAGACTACTTACCGCGCATAAAGAATCCAGCACAGAATCTGAACCAAACATTGGCAAGATCAGAGATGCAGCTGCTGCCAGGAAACAACCAAAAAGAAACAGCAACCGCCGAACCTCTGCATTGAATGGTCACACAAGTAACCCAGGTGATGATGACTATTGGACTCCATTGAGCAATGCTCTTCCAGCTGCTGCCTCAACCTCCCAAAGCAACTCCAACGTCTCGAGATCATCAGAACCTAATTCACCTGCAGCAGGTATTGGTGGCAAAAGATGGAAGCAAACAACACCTGTAGGTGATAACATTCGAAGAAAGAGCAACCCAATTAGATGGGTGGCTCCAACGGGACCAGCAATGGTTGCTTCATTTCTTATAGCTAGATTACAAGGAGATGAAGTCGCAACAGTTAACGGTAGTACTAGTACAGGCGGTGGTGGTTTGACAGAACATGTATATGGCTCTTTAGCACTGGACATAGTCAACAGCTCTTGGTTACAGATCATATTGGCAGGGATTACCTGGTATATAATTGGAACAATAATTGTAGGACTTGCAGATGCCATTCAAAGTAGGGAAAGTAGGAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ Fer4_13

Protein Analysis

391

Amino Acids

42.51

Weight (kDa)

9.42

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer4_15 PF13459 32 - 89 3.2e-06 4Fe-4S single cluster domain
Fer4_13 PF13370 34 - 89 3.6e-13 4Fe-4S single cluster domain of Ferredoxin I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014546)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23240
fragaria_vesca FvH4_4g37080
malus_domestica MD13G1003800.v1.1
prunus_persica Prupe.1G348700_v2.0.a1
pyrus_communis pycom13g00320
rosa_chinensis RchiOBHm_Chr4g0446831
rosa_laevigata RLG00000005626
rosa_multiflora Rmu_co8372733.1_g000001 Rmu_sc0001753.1_g000016 Rmu_sc0001753.1_g000019
rosa_roxburghii Rroxscaffold_5G00387200
rosa_rugosa Rorug04G0375000
rosa_samantha Rh4AG436500 Rh4BG445600 Rh4CG462900 Rh4DG444300
rosa_wichuraiana Rw4G037340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 530, 841
Acc36I ACCTGC 3 cut(s) 530, 830, 841
AccB7I CCANNNNNTGG 1 cut(s) 635
AccI GTMKAC 1 cut(s) 358
AccII CGCG 1 cut(s) 599
AciI CCGC 3 cut(s) 597, 691, 1014
AclWI GGATC 1 cut(s) 492
AcsI RAATTY 3 cut(s) 296, 346, 392
AcvI CACGTG 1 cut(s) 320
AfaI GTAC 3 cut(s) 562, 1003, 1009
AfiI CCNNNNNNNGG 2 cut(s) 635, 839
AflIII ACRYGT 1 cut(s) 1033
AgsI TTSAA 6 cut(s) 130, 167, 397, 488, 709, 1151
AhlI ACTAGT 1 cut(s) 1004
AjnI CCWGG 4 cut(s) 185, 662, 727, 1103
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AluBI AGCT 7 cut(s) 28, 290, 428, 656, 773, 962, 1071
AluI AGCT 7 cut(s) 28, 290, 428, 656, 773, 962, 1071
Alw21I GWGCWC 2 cut(s) 12, 30
Alw26I GTCTC 3 cut(s) 370, 443, 811
AlwI GGATC 1 cut(s) 492
AlwNI CAGNNNCTG 3 cut(s) 623, 659, 776
Ama87I CYCGRG 1 cut(s) 808
ApeKI GCWGC 8 cut(s) 287, 313, 653, 656, 659, 773, 776, 836
ApoI RAATTY 3 cut(s) 296, 346, 392
Asp700I GAANNNNTTC 1 cut(s) 392
AspLEI GCGC 1 cut(s) 601
AspS9I GGNCC 2 cut(s) 280, 932
AsuHPI GGTGA 5 cut(s) 397, 554, 743, 822, 893
AsuII TTCGAA 1 cut(s) 892
AvaI CYCGRG 1 cut(s) 808
AvaII GGWCC 2 cut(s) 280, 932
BanII GRGCYC 1 cut(s) 30
BarI GAAGNNNNNNTAC 2 cut(s) 38, 70
BbrPI CACGTG 1 cut(s) 320
Bbv12I GWGCWC 2 cut(s) 12, 30
BbvI GCAGC 8 cut(s) 299, 325, 643, 646, 665, 760, 763, 848
BccI CCATC 4 cut(s) 500, 509, 852, 909
BciT130I CCWGG 4 cut(s) 187, 664, 729, 1105
BcoDI GTCTC 3 cut(s) 370, 443, 811
BcuI ACTAGT 1 cut(s) 1004
BfaI CTAG 3 cut(s) 284, 963, 1005
BfmI CTRYAG 2 cut(s) 834, 876
BfuAI ACCTGC 3 cut(s) 530, 830, 841
BisI GCNGC 8 cut(s) 288, 314, 654, 657, 660, 774, 777, 837
BlsI GCNGC 8 cut(s) 289, 315, 655, 658, 661, 775, 778, 838
BmcAI AGTACT 1 cut(s) 1003
Bme1390I CCNGG 4 cut(s) 187, 664, 729, 1105
Bme18I GGWCC 2 cut(s) 280, 932
BmeT110I CYCGRG 1 cut(s) 808
BmgT120I GGNCC 2 cut(s) 280, 932
BmiI GGNNCC 2 cut(s) 924, 933
BmrFI CCNGG 4 cut(s) 187, 664, 729, 1105
BmsI GCATC 4 cut(s) 267, 403, 640, 1132
BplI GAGNNNNNCTC 2 cut(s) 748, 780
Bpu14I TTCGAA 1 cut(s) 892
BsaAI YACGTR 1 cut(s) 320
BsaJI CCNNGG 3 cut(s) 31, 186, 727
BsaXI ACNNNNNCTCC 2 cut(s) 966, 996
Bsc4I CCNNNNNNNGG 2 cut(s) 635, 839
Bse118I RCCGGY 1 cut(s) 353
Bse1I ACTGG 3 cut(s) 515, 549, 1059
Bse3DI GCAATG 3 cut(s) 331, 766, 945
BseBI CCWGG 4 cut(s) 187, 664, 729, 1105
BseDI CCNNGG 3 cut(s) 31, 186, 727
BseGI GGATG 1 cut(s) 109
BseLI CCNNNNNNNGG 2 cut(s) 635, 839
BseMI GCAATG 3 cut(s) 331, 766, 945
BseMII CTCAG 1 cut(s) 54
BseNI ACTGG 3 cut(s) 515, 549, 1059
BseXI GCAGC 8 cut(s) 299, 325, 643, 646, 665, 760, 763, 848
BsgI GTGCAG 2 cut(s) 417, 558
Bsh1236I CGCG 1 cut(s) 599
BsiHKAI GWGCWC 2 cut(s) 12, 30
BsiHKCI CYCGRG 1 cut(s) 808
BsiSI CCGG 1 cut(s) 354
BslFI GGGAC 1 cut(s) 945
BslI CCNNNNNNNGG 2 cut(s) 635, 839
BsmAI GTCTC 3 cut(s) 370, 443, 811
BsmBI CGTCTC 1 cut(s) 811
BsmFI GGGAC 1 cut(s) 945
BsoBI CYCGRG 1 cut(s) 808
Bsp119I TTCGAA 1 cut(s) 892
Bsp1286I GDGCHC 2 cut(s) 12, 30
Bsp143I GATC 4 cut(s) 497, 642, 812, 1083
BspACI CCGC 3 cut(s) 597, 691, 1014
BspCNI CTCAG 1 cut(s) 53
BspFNI CGCG 1 cut(s) 599
BspLI GGNNCC 2 cut(s) 924, 933
BspMAI CTGCAG 1 cut(s) 838
BspMI ACCTGC 3 cut(s) 530, 830, 841
BspPI GGATC 1 cut(s) 492
BspQI GCTCTTC 2 cut(s) 175, 771
BspT104I TTCGAA 1 cut(s) 892
BsrDI GCAATG 3 cut(s) 331, 766, 945
BsrFI RCCGGY 1 cut(s) 353
BsrI ACTGG 3 cut(s) 515, 549, 1059
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 3 cut(s) 31, 186, 727
BssMI GATC 4 cut(s) 497, 642, 812, 1083
BssNAI GTATAC 1 cut(s) 359
Bst1107I GTATAC 1 cut(s) 359
Bst2UI CCWGG 4 cut(s) 187, 664, 729, 1105
Bst4CI ACNGT 3 cut(s) 201, 991, 998
Bst6I CTCTTC 2 cut(s) 175, 771
BstAPI GCANNNNNTGC 2 cut(s) 659, 944
BstBAI YACGTR 1 cut(s) 320
BstBI TTCGAA 1 cut(s) 892
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 1 cut(s) 40
BstENI CCTNNNNNAGG 1 cut(s) 837
BstF5I GGATG 1 cut(s) 109
BstFNI CGCG 1 cut(s) 599
BstHHI GCGC 1 cut(s) 601
BstKTI GATC 4 cut(s) 500, 645, 815, 1086
BstMAI GTCTC 3 cut(s) 370, 443, 811
BstMBI GATC 4 cut(s) 497, 642, 812, 1083
BstMWI GCNNNNNNNGC 6 cut(s) 143, 239, 488, 659, 770, 944
BstNI CCWGG 4 cut(s) 187, 664, 729, 1105
BstNSI RCATGY 1 cut(s) 1037
BstSCI CCNGG 4 cut(s) 185, 662, 727, 1103
BstSFI CTRYAG 2 cut(s) 834, 876
BstUI CGCG 1 cut(s) 599
BstV1I GCAGC 8 cut(s) 299, 325, 643, 646, 665, 760, 763, 848
BstX2I RGATCY 1 cut(s) 497
BstXI CCANNNNNNTGG 1 cut(s) 915
BstYI RGATCY 1 cut(s) 497
BstZ17I GTATAC 1 cut(s) 359
BtsCI GGATG 1 cut(s) 109
BtsIMutI CAGTG 2 cut(s) 206, 1052
BveI ACCTGC 3 cut(s) 530, 830, 841
Cac8I GCNNGC 1 cut(s) 231
CaiI CAGNNNCTG 3 cut(s) 623, 659, 776
CfoI GCGC 1 cut(s) 601
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 2 cut(s) 280, 932
CsiI ACCWGGT 1 cut(s) 1103
Csp6I GTAC 3 cut(s) 561, 1002, 1008
CviAII CATG 2 cut(s) 63, 1034
CviQI GTAC 3 cut(s) 561, 1002, 1008
DdeI CTNAG 1 cut(s) 40
DpnI GATC 4 cut(s) 499, 644, 814, 1085
DpnII GATC 4 cut(s) 497, 642, 812, 1083
Eam1104I CTCTTC 2 cut(s) 175, 771
EarI CTCTTC 2 cut(s) 175, 771
Ecl136II GAGCTC 1 cut(s) 28
Eco24I GRGCYC 1 cut(s) 30
Eco47I GGWCC 2 cut(s) 280, 932
Eco53kI GAGCTC 1 cut(s) 28
Eco72I CACGTG 1 cut(s) 320
Eco88I CYCGRG 1 cut(s) 808
EcoICRI GAGCTC 1 cut(s) 28
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 4 cut(s) 185, 662, 727, 1103
EcoT38I GRGCYC 1 cut(s) 30
Esp3I CGTCTC 1 cut(s) 811
FaeI CATG 2 cut(s) 66, 1037
FaqI GGGAC 1 cut(s) 945
FatI CATG 2 cut(s) 62, 1033
FblI GTMKAC 1 cut(s) 358
Fnu4HI GCNGC 8 cut(s) 288, 314, 654, 657, 660, 774, 777, 837
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 8 cut(s) 288, 314, 654, 657, 660, 774, 777, 837
FspBI CTAG 3 cut(s) 284, 963, 1005
GlaI GCGC 1 cut(s) 600
GluI GCNGC 8 cut(s) 288, 314, 654, 657, 660, 774, 777, 837
HapII CCGG 1 cut(s) 354
HhaI GCGC 1 cut(s) 601
Hin1II CATG 2 cut(s) 66, 1037
Hin6I GCGC 1 cut(s) 599
HinP1I GCGC 1 cut(s) 599
HincII GTYRAC 2 cut(s) 994, 1066
HindII GTYRAC 2 cut(s) 994, 1066
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 6 cut(s) 167, 363, 405, 608, 620, 748
HpaI GTTAAC 1 cut(s) 994
HpaII CCGG 1 cut(s) 354
HphI GGTGA 5 cut(s) 397, 554, 743, 822, 893
Hpy166II GTNNAC 3 cut(s) 359, 994, 1066
Hpy188I TCNGA 8 cut(s) 32, 76, 197, 280, 497, 625, 647, 820
Hpy188III TCNNGA 5 cut(s) 293, 397, 555, 808, 810
Hpy8I GTNNAC 3 cut(s) 359, 994, 1066
HpyCH4III ACNGT 3 cut(s) 201, 991, 998
HpyCH4IV ACGT 2 cut(s) 319, 804
HpyF10VI GCNNNNNNNGC 6 cut(s) 143, 239, 488, 659, 770, 944
HpyF3I CTNAG 1 cut(s) 40
HpySE526I ACGT 2 cut(s) 319, 804
Hsp92II CATG 2 cut(s) 66, 1037
HspAI GCGC 1 cut(s) 599
KspAI GTTAAC 1 cut(s) 994
Kzo9I GATC 4 cut(s) 497, 642, 812, 1083
LguI GCTCTTC 2 cut(s) 175, 771
LmnI GCTCC 3 cut(s) 7, 33, 928
Lsp1109I GCAGC 8 cut(s) 299, 325, 643, 646, 665, 760, 763, 848
LweI GCATC 4 cut(s) 267, 403, 640, 1132
MabI ACCWGGT 1 cut(s) 1103
MaeI CTAG 3 cut(s) 284, 963, 1005
MaeII ACGT 2 cut(s) 319, 804
MaeIII GTNAC 4 cut(s) 35, 713, 722, 1077
MalI GATC 4 cut(s) 499, 644, 814, 1085
MboI GATC 4 cut(s) 497, 642, 812, 1083
MboII GAAGA 4 cut(s) 95, 192, 758, 906
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 1 cut(s) 497
MhlI GDGCHC 2 cut(s) 12, 30
MlyI GAGTC 2 cut(s) 372, 742
MmeI TCCRAC 7 cut(s) 175, 258, 393, 531, 601, 825, 950
MnlI CCTC 8 cut(s) 26, 60, 267, 376, 582, 709, 790, 796
MroXI GAANNNNTTC 1 cut(s) 392
MseI TTAA 2 cut(s) 584, 993
MslI CAYNNNNRTG 1 cut(s) 1038
MspA1I CMGCKG 2 cut(s) 656, 773
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 4 cut(s) 187, 664, 729, 1105
MunI CAATTG 1 cut(s) 162
MvaI CCWGG 4 cut(s) 187, 664, 729, 1105
MvnI CGCG 1 cut(s) 599
MwoI GCNNNNNNNGC 6 cut(s) 143, 239, 488, 659, 770, 944
NdeII GATC 4 cut(s) 497, 642, 812, 1083
NlaIII CATG 2 cut(s) 66, 1037
NlaIV GGNNCC 2 cut(s) 924, 933
NmuCI GTSAC 1 cut(s) 713
NspI RCATGY 1 cut(s) 1037
NspV TTCGAA 1 cut(s) 892
PaeR7I CTCGAG 1 cut(s) 808
PaqCI CACCTGC 2 cut(s) 530, 841
PciI ACATGT 1 cut(s) 1033
PciSI GCTCTTC 2 cut(s) 175, 771
PdmI GAANNNNTTC 1 cut(s) 392
PfeI GAWTC 4 cut(s) 167, 405, 608, 620
PflFI GACNNNGTC 1 cut(s) 1061
PflMI CCANNNNNTGG 1 cut(s) 635
PkrI GCNGC 8 cut(s) 289, 315, 655, 658, 661, 775, 778, 838
PleI GAGTC 2 cut(s) 371, 742
PmaCI CACGTG 1 cut(s) 320
PmlI CACGTG 1 cut(s) 320
PpsI GAGTC 2 cut(s) 371, 742
Ppu21I YACGTR 1 cut(s) 320
PscI ACATGT 1 cut(s) 1033
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 4 cut(s) 185, 662, 727, 1103
PspCI CACGTG 1 cut(s) 320
PspGI CCWGG 4 cut(s) 185, 662, 727, 1103
PspN4I GGNNCC 2 cut(s) 924, 933
PspPI GGNCC 2 cut(s) 280, 932
PsrI GAACNNNNNNTAC 2 cut(s) 207, 239
PstI CTGCAG 1 cut(s) 838
PstNI CAGNNNCTG 3 cut(s) 623, 659, 776
PsuI RGATCY 1 cut(s) 497
PsyI GACNNNGTC 1 cut(s) 1061
PvuII CAGCTG 2 cut(s) 656, 773
RsaI GTAC 3 cut(s) 562, 1003, 1009
RsaNI GTAC 3 cut(s) 561, 1002, 1008
RseI CAYNNNNRTG 1 cut(s) 1038
SacI GAGCTC 1 cut(s) 30
SapI GCTCTTC 2 cut(s) 175, 771
SaqAI TTAA 2 cut(s) 584, 993
SatI GCNGC 8 cut(s) 288, 314, 654, 657, 660, 774, 777, 837
Sau3AI GATC 4 cut(s) 497, 642, 812, 1083
Sau96I GGNCC 2 cut(s) 280, 932
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 2 cut(s) 372, 742
ScrFI CCNGG 4 cut(s) 187, 664, 729, 1105
SduI GDGCHC 2 cut(s) 12, 30
SexAI ACCWGGT 1 cut(s) 1103
SfaNI GCATC 4 cut(s) 267, 403, 640, 1132
SfcI CTRYAG 2 cut(s) 834, 876
Sfr274I CTCGAG 1 cut(s) 808
SfuI TTCGAA 1 cut(s) 892
SinI GGWCC 2 cut(s) 280, 932
SlaI CTCGAG 1 cut(s) 808
SmiMI CAYNNNNRTG 1 cut(s) 1038
SmlI CTYRAG 1 cut(s) 808
SmoI CTYRAG 1 cut(s) 808
SpeI ACTAGT 1 cut(s) 1004
SsiI CCGC 3 cut(s) 597, 691, 1014
SspMI CTAG 3 cut(s) 284, 963, 1005
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 4 cut(s) 185, 662, 727, 1103
TaaI ACNGT 3 cut(s) 201, 991, 998
TaiI ACGT 2 cut(s) 322, 807
TaqI TCGA 3 cut(s) 14, 809, 892
TatI WGTACW 3 cut(s) 560, 1001, 1007
TfiI GAWTC 4 cut(s) 167, 405, 608, 620
Tru1I TTAA 2 cut(s) 584, 993
Tru9I TTAA 2 cut(s) 584, 993
TscAI CASTG 2 cut(s) 206, 1059
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 8 cut(s) 287, 313, 653, 656, 659, 773, 776, 836
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 3 cut(s) 120, 939, 993
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 2 cut(s) 206, 1059
Tth111I GACNNNGTC 1 cut(s) 1061
Van91I CCANNNNNTGG 1 cut(s) 635
VpaK11BI GGWCC 2 cut(s) 280, 932
XagI CCTNNNNNAGG 1 cut(s) 837
XapI RAATTY 3 cut(s) 296, 346, 392
XceI RCATGY 1 cut(s) 1037
XhoI CTCGAG 1 cut(s) 808
XmiI GTMKAC 1 cut(s) 358
XmnI GAANNNNTTC 1 cut(s) 392
XspI CTAG 3 cut(s) 284, 963, 1005
ZrmI AGTACT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.