Rh4CG067300

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008407
Physical Location & Seq
Forward (+)
12620554 .. 12625217
4664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG067300.1

Sequence Viewer

Length: 1236 bp
ATGGCAGCCATGGAAGGCGCGCTACAGAACCAACCTCCACAGCCCCTCCAACAGCAGCAACCGCAACCCGCGGCGGTGGAGAGACTAAACGACGCCGTTCAGCAGCAGCTGAACCTCAAGTCCGTCAAGGACCGAGCCACCAACCTGTTCAAAAACATCACCCGCATTCTCGAAGACCTCGACGGCCACGCTCGCACCAACACCACTCCCAAATGGCAGGACCTCCTGAGCCAGTACTCCATGGTCAACCTCGAGCTCTTCAACATCGTCGACGAAATCAAAAAGGTCTCCAAGGCCTTCGTGGTCTATCCCAAGAATGTGAACGCCGAGAACGCCGTGGTGTTGCCGGTGATGCTTTCGTCGAAGCTCTTGCCGGAGATGGAGGTCGAGGACAACGCCAAGAGAGAGCAGTTGCTTCTAGGGTTGCAGAGCCTCCCAATCTCGACCCAAATTGACAAGCTAAAGGCTAGGATTGATATGATTGGAGCTGCTTGTGAGAGTGCCGAGAAAGTATTGGCAGACACTCGAAAAGCCTACTGCATTGGTACTCGGCAAGGCATTCCGGTTGTTCCTACTCTGGACAAAGCTCAGGCAGCTAAAATTCAAGAGCAGGAGAATCTACTCCGGGCTGCTGTTAATTATGGGGACGCTTTACGGTTGCCTCCAGACCAAAGGCAGAATCCGCCTGCACTTCCAATGCATCTGGTAGATGTGCTCGGTGGCGGTGATGGTGGTGCGCAAGCATTTCCTGAAGCGTCTGGTATGTACTCAAAGAATACTCCTGTATCATCAAGTAACTTGTCTGGCCAGAGCCCGTTGATGCAGGTTCCCGGATCACAACTTATGGGAAGATCAGCTGCATCTCCTGGTGGCACAAGTATTACAAACTTCGATAACACAACAACATCTCCATTACCATATGCCAACTCCCCAAGGTCTAGTACAAACATTATGAATACGCCATCTCCTCAACAACAAAGCCAGCAACAGAAGCAGCAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGAAGTTGATGCAATTACCTCCTCAACAGCAAATCCTGGCTCAGCAACAATTTAGGCAATCAGCAATGCAAGGACTTGGACAAAACCCACTGCATCAGCTGCATGATTTGCAGGGACAGGCTCAGCAAAAGTTCCAGTCGGTAAATTTGTTGCCTAGCTTCTTTTTTCTTGTACTTCTTTTTTCAGGAGCCTGTTGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

411

Amino Acids

45.24

Weight (kDa)

8.33

Isoelectric Point (pI)

65.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 738
Acc36I ACCTGC 1 cut(s) 814
AccI GTMKAC 1 cut(s) 270
AccII CGCG 2 cut(s) 20, 71
AciI CCGC 7 cut(s) 62, 69, 71, 74, 163, 683, 723
AclWI GGATC 1 cut(s) 841
AcoI YGGCCR 3 cut(s) 184, 805, 1229
AcsI RAATTY 2 cut(s) 600, 1177
AcuI CTGAAG 1 cut(s) 771
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 5 cut(s) 236, 547, 767, 943, 1206
AgsI TTSAA 3 cut(s) 151, 262, 605
AjnI CCWGG 2 cut(s) 865, 1068
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
Alw21I GWGCWC 2 cut(s) 258, 717
Alw26I GTCTC 2 cut(s) 76, 292
AlwI GGATC 1 cut(s) 841
AlwNI CAGNNNCTG 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 4 cut(s) 184, 294, 805, 1229
ApoI RAATTY 2 cut(s) 600, 1177
AspLEI GCGC 3 cut(s) 20, 22, 739
AspS9I GGNCC 2 cut(s) 130, 220
AsuC2I CCSGG 2 cut(s) 626, 831
AsuHPI GGTGA 3 cut(s) 151, 361, 737
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 2 cut(s) 130, 220
BaeI ACNNNNGTAYC 2 cut(s) 768, 801
BalI TGGCCA 2 cut(s) 807, 1231
BanII GRGCYC 2 cut(s) 258, 815
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 2 cut(s) 258, 717
BccI CCATC 3 cut(s) 373, 722, 970
BceAI ACGGC 3 cut(s) 80, 199, 320
BcgI CGANNNNNNTGC 2 cut(s) 352, 386
BciT130I CCWGG 2 cut(s) 867, 1070
BcnI CCSGG 2 cut(s) 626, 831
BcoDI GTCTC 2 cut(s) 76, 292
BfaI CTAG 4 cut(s) 419, 468, 939, 1188
BfmI CTRYAG 1 cut(s) 23
BfuAI ACCTGC 1 cut(s) 814
BlpI GCTNAGC 2 cut(s) 1074, 1155
BmcAI AGTACT 1 cut(s) 236
Bme1390I CCNGG 4 cut(s) 626, 831, 867, 1070
Bme18I GGWCC 2 cut(s) 130, 220
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 2 cut(s) 130, 220
BmiI GGNNCC 2 cut(s) 828, 1222
BmrFI CCNGG 4 cut(s) 626, 831, 867, 1070
BmsI GCATC 6 cut(s) 342, 709, 810, 869, 1032, 1135
BpiI GAAGAC 1 cut(s) 180
BpmI CTGGAG 1 cut(s) 648
Bpu10I CCTNAGC 2 cut(s) 227, 588
Bpu1102I GCTNAGC 2 cut(s) 1074, 1155
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 2 cut(s) 626, 831
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 292
BsaJI CCNNGG 6 cut(s) 9, 69, 240, 291, 336, 932
BsaWI WCCGGW 1 cut(s) 562
BsaXI ACNNNNNCTCC 6 cut(s) 30, 60, 892, 922, 949, 979
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 2 cut(s) 232, 1168
Bse3DI GCAATG 1 cut(s) 1104
BseBI CCWGG 2 cut(s) 867, 1070
BseDI CCNNGG 6 cut(s) 9, 69, 240, 291, 336, 932
BseMI GCAATG 1 cut(s) 1104
BseMII CTCAG 4 cut(s) 218, 602, 1088, 1169
BseNI ACTGG 2 cut(s) 232, 1168
BsePI GCGCGC 1 cut(s) 18
BseRI GAGGAG 2 cut(s) 957, 1044
BsgI GTGCAG 1 cut(s) 672
Bsh1236I CGCG 2 cut(s) 20, 71
BshFI GGCC 4 cut(s) 186, 296, 807, 1231
BsiHKAI GWGCWC 2 cut(s) 258, 717
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 5 cut(s) 347, 374, 563, 625, 831
BslFI GGGAC 2 cut(s) 659, 1161
BsmAI GTCTC 2 cut(s) 76, 292
BsmFI GGGAC 2 cut(s) 659, 1161
BsmI GAATGC 2 cut(s) 165, 558
BsnI GGCC 4 cut(s) 186, 296, 807, 1231
Bso31I GGTCTC 1 cut(s) 292
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 3 cut(s) 258, 717, 815
Bsp143I GATC 2 cut(s) 833, 851
Bsp1720I GCTNAGC 2 cut(s) 1074, 1155
Bsp19I CCATGG 2 cut(s) 9, 240
BspACI CCGC 7 cut(s) 62, 69, 71, 74, 163, 683, 723
BspANI GGCC 4 cut(s) 186, 296, 807, 1231
BspCNI CTCAG 4 cut(s) 219, 601, 1087, 1168
BspFNI CGCG 2 cut(s) 20, 71
BspLI GGNNCC 2 cut(s) 828, 1222
BspMI ACCTGC 1 cut(s) 814
BspPI GGATC 1 cut(s) 841
BspQI GCTCTTC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 1104
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 2 cut(s) 232, 1168
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 6 cut(s) 9, 69, 240, 291, 336, 932
BssHII GCGCGC 1 cut(s) 18
BssMI GATC 2 cut(s) 833, 851
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 4 cut(s) 9, 240, 291, 932
Bst2UI CCWGG 2 cut(s) 867, 1070
Bst4CI ACNGT 1 cut(s) 657
Bst6I CTCTTC 1 cut(s) 263
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 2 cut(s) 1132, 1141
BstC8I GCNNGC 5 cut(s) 20, 193, 687, 741, 983
BstDEI CTNAG 4 cut(s) 227, 588, 1074, 1155
BstDSI CCRYGG 4 cut(s) 9, 69, 240, 336
BstFNI CGCG 2 cut(s) 20, 71
BstHHI GCGC 3 cut(s) 20, 22, 739
BstKTI GATC 2 cut(s) 836, 854
BstMAI GTCTC 2 cut(s) 76, 292
BstMBI GATC 2 cut(s) 833, 851
BstNI CCWGG 2 cut(s) 867, 1070
BstSCI CCNGG 4 cut(s) 624, 829, 865, 1068
BstSFI CTRYAG 1 cut(s) 23
BstUI CGCG 2 cut(s) 20, 71
BstV2I GAAGAC 1 cut(s) 180
BsuRI GGCC 4 cut(s) 186, 296, 807, 1231
BtgI CCRYGG 4 cut(s) 9, 69, 240, 336
BtsI GCAGTG 1 cut(s) 1121
BtsIMutI CAGTG 1 cut(s) 1121
BveI ACCTGC 1 cut(s) 814
Cac8I GCNNGC 5 cut(s) 20, 193, 687, 741, 983
CaiI CAGNNNCTG 1 cut(s) 109
CfoI GCGC 3 cut(s) 20, 22, 739
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 2 cut(s) 130, 220
Cfr42I CCGCGG 1 cut(s) 72
CseI GACGC 3 cut(s) 101, 656, 744
Csp6I GTAC 5 cut(s) 235, 546, 766, 942, 1205
CviAII CATG 4 cut(s) 10, 241, 1136, 1233
CviQI GTAC 5 cut(s) 235, 546, 766, 942, 1205
DdeI CTNAG 4 cut(s) 227, 588, 1074, 1155
DpnI GATC 2 cut(s) 835, 853
DpnII GATC 2 cut(s) 833, 851
EaeI YGGCCR 3 cut(s) 184, 805, 1229
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
EciI GGCGGA 1 cut(s) 672
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 4 cut(s) 9, 240, 291, 932
Eco147I AGGCCT 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 258, 815
Eco31I GGTCTC 1 cut(s) 292
Eco47I GGWCC 2 cut(s) 130, 220
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 771
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 220
EcoRII CCWGG 2 cut(s) 865, 1068
EcoT14I CCWWGG 4 cut(s) 9, 240, 291, 932
EcoT22I ATGCAT 1 cut(s) 702
EcoT38I GRGCYC 2 cut(s) 258, 815
ErhI CCWWGG 4 cut(s) 9, 240, 291, 932
FaeI CATG 4 cut(s) 13, 244, 1139, 1236
FaqI GGGAC 2 cut(s) 659, 1161
FatI CATG 4 cut(s) 9, 240, 1135, 1232
FauI CCCGC 2 cut(s) 76, 170
FauNDI CATATG 1 cut(s) 919
FblI GTMKAC 1 cut(s) 270
FriOI GRGCYC 2 cut(s) 258, 815
FspBI CTAG 4 cut(s) 419, 468, 939, 1188
FspI TGCGCA 1 cut(s) 738
GlaI GCGC 3 cut(s) 19, 21, 738
GsuI CTGGAG 1 cut(s) 648
HaeIII GGCC 4 cut(s) 186, 296, 807, 1231
HapII CCGG 5 cut(s) 347, 374, 563, 625, 831
HgaI GACGC 3 cut(s) 101, 656, 744
HhaI GCGC 3 cut(s) 20, 22, 739
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 4 cut(s) 13, 244, 1139, 1236
Hin6I GCGC 3 cut(s) 18, 20, 737
HinP1I GCGC 3 cut(s) 18, 20, 737
HincII GTYRAC 2 cut(s) 247, 271
HindII GTYRAC 2 cut(s) 247, 271
HinfI GANTC 2 cut(s) 616, 679
HpaII CCGG 5 cut(s) 347, 374, 563, 625, 831
HphI GGTGA 3 cut(s) 151, 361, 737
Hpy166II GTNNAC 3 cut(s) 247, 271, 322
Hpy188III TCNNGA 8 cut(s) 170, 226, 442, 578, 605, 665, 749, 1218
Hpy8I GTNNAC 3 cut(s) 247, 271, 322
Hpy99I CGWCG 5 cut(s) 95, 185, 272, 275, 364
HpyAV CCTTC 2 cut(s) 8, 307
HpyCH4III ACNGT 1 cut(s) 657
HpyF3I CTNAG 4 cut(s) 227, 588, 1074, 1155
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 4 cut(s) 13, 244, 1139, 1236
HspAI GCGC 3 cut(s) 18, 20, 737
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 2 cut(s) 833, 851
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 2 cut(s) 485, 1220
LweI GCATC 6 cut(s) 342, 709, 810, 869, 1032, 1135
MaeI CTAG 4 cut(s) 419, 468, 939, 1188
MaeIII GTNAC 1 cut(s) 794
MalI GATC 2 cut(s) 835, 853
MboI GATC 2 cut(s) 833, 851
MboII GAAGA 3 cut(s) 185, 250, 861
MhlI GDGCHC 3 cut(s) 258, 717, 815
MlsI TGGCCA 2 cut(s) 807, 1231
MluCI AATT 6 cut(s) 450, 600, 637, 1046, 1082, 1177
MluNI TGGCCA 2 cut(s) 807, 1231
MmeI TCCRAC 1 cut(s) 73
Mox20I TGGCCA 2 cut(s) 807, 1231
Mph1103I ATGCAT 1 cut(s) 702
MscI TGGCCA 2 cut(s) 807, 1231
MseI TTAA 1 cut(s) 636
Msp20I TGGCCA 2 cut(s) 807, 1231
MspA1I CMGCKG 4 cut(s) 71, 109, 857, 1132
MspI CCGG 5 cut(s) 347, 374, 563, 625, 831
MspR9I CCNGG 4 cut(s) 626, 831, 867, 1070
Mva1269I GAATGC 2 cut(s) 165, 558
MvaI CCWGG 2 cut(s) 867, 1070
MvnI CGCG 2 cut(s) 20, 71
NciI CCSGG 2 cut(s) 626, 831
NcoI CCATGG 2 cut(s) 9, 240
NdeI CATATG 1 cut(s) 919
NdeII GATC 2 cut(s) 833, 851
NlaIII CATG 4 cut(s) 13, 244, 1139, 1236
NlaIV GGNNCC 2 cut(s) 828, 1222
NmeAIII GCCGAG 3 cut(s) 352, 529, 529
NsbI TGCGCA 1 cut(s) 738
NsiI ATGCAT 1 cut(s) 702
PaeR7I CTCGAG 1 cut(s) 251
PauI GCGCGC 1 cut(s) 18
PceI AGGCCT 1 cut(s) 296
PciSI GCTCTTC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 2 cut(s) 165, 558
PfeI GAWTC 2 cut(s) 616, 679
PfoI TCCNGGA 1 cut(s) 829
PpuMI RGGWCCY 1 cut(s) 220
Psp124BI GAGCTC 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 2 cut(s) 865, 1068
PspGI CCWGG 2 cut(s) 865, 1068
PspN4I GGNNCC 2 cut(s) 828, 1222
PspPI GGNCC 2 cut(s) 130, 220
PspPPI RGGWCCY 1 cut(s) 220
PspXI VCTCGAGB 1 cut(s) 251
PstNI CAGNNNCTG 1 cut(s) 109
PteI GCGCGC 1 cut(s) 18
PvuII CAGCTG 3 cut(s) 109, 857, 1132
RsaI GTAC 5 cut(s) 236, 547, 767, 943, 1206
RsaNI GTAC 5 cut(s) 235, 546, 766, 942, 1205
SacI GAGCTC 1 cut(s) 258
SacII CCGCGG 1 cut(s) 72
SalI GTCGAC 1 cut(s) 269
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 636
Sau3AI GATC 2 cut(s) 833, 851
Sau96I GGNCC 2 cut(s) 130, 220
ScaI AGTACT 1 cut(s) 236
ScrFI CCNGG 4 cut(s) 626, 831, 867, 1070
SduI GDGCHC 3 cut(s) 258, 717, 815
SfaNI GCATC 6 cut(s) 342, 709, 810, 869, 1032, 1135
SfcI CTRYAG 1 cut(s) 23
Sfr274I CTCGAG 1 cut(s) 251
Sfr303I CCGCGG 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 72
SgrDI CGTCGACG 1 cut(s) 269
SinI GGWCC 2 cut(s) 130, 220
SlaI CTCGAG 1 cut(s) 251
SmlI CTYRAG 2 cut(s) 116, 251
SmoI CTYRAG 2 cut(s) 116, 251
Sse9I AATT 6 cut(s) 450, 600, 637, 1046, 1082, 1177
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 7 cut(s) 62, 69, 71, 74, 163, 683, 723
SspMI CTAG 4 cut(s) 419, 468, 939, 1188
SstI GAGCTC 1 cut(s) 258
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 4 cut(s) 624, 829, 865, 1068
StyI CCWWGG 4 cut(s) 9, 240, 291, 932
TaaI ACNGT 1 cut(s) 657
TaqI TCGA 9 cut(s) 171, 180, 252, 270, 362, 387, 443, 526, 891
TaqII GACCGA 1 cut(s) 147
TasI AATT 6 cut(s) 450, 600, 637, 1046, 1082, 1177
TatI WGTACW 4 cut(s) 234, 765, 941, 1204
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 2 cut(s) 616, 679
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 1 cut(s) 1128
TspDTI ATGAA 1 cut(s) 968
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 1 cut(s) 1128
VpaK11BI GGWCC 2 cut(s) 130, 220
XapI RAATTY 2 cut(s) 600, 1177
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 4 cut(s) 419, 468, 939, 1188
ZrmI AGTACT 1 cut(s) 236
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.