Rh4DG056500

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008407
Physical Location & Seq
Forward (+)
8720257 .. 8724984
4728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG056500.1

Sequence Viewer

Length: 1374 bp
ATGGCAGCCATGGAAGGCGCGCTACAGAACCAACCTCCACAGCCCCTCCAACAGCAACAACCGCAACCCGCGGCGGTGGAGAGACTAAACGACGCCGTACAGCAGCAGCTGAACCTCAAGTCCGTCAAGGACCGAGCCACCAACCTGTTCAAAAACATCACCCGCATTCTCGAAGACCTCGACGGCCACGCTCGCACCAACACCACTCCCAAATGGCAGGACCTCCTGAGCCAGTACTCCATGGTCAACCTGGAGCTCTTCAACATCGTCGACGAAATCAAAAAGGTCTCCAAGGCCTTCGTGGTCTATCCCAAGAATGTGAACGCCGAGAACGCCGTGGTGTTGCCGGTGATGCTTTCGTCGAAGCTATTGCCGGAGATGGAGGTCGAGGACAATGCTAAGAGAGAGCAGTTGCTTCTAGGGTTGCAGAGCCTCCCAATCTCGACCCAAATTGACAAGCTAAAGGCTAGGATTGATATGATTGGAGCTGCTTGTGAGAGTGCCGAGAAAGTATTGGCAGACACTCGGAAAGCCTACTGCATTGGAACTCGGCAAGGCATTCCGGTTGTTCCTACTCTGGACAAAGCTCAGGCAGCTAAAATTCAAGAGCAGGAGAATCTACTCCGGGCTGCTGTTAATTATGGGGACGCTTTACGGTTACCTCCAGACCAAAGGCAGAATCCACCTGCACTTCCAATGCATCTGGTAGATGTGCTCGGTGGCGGTGGTGGTGGTGATGGTGGTGTGCAAGCATTTCCTGAAGCGTCTGGTATGTACTCAAAGAATACTCCTGTATCATCAAGTAACTTGTCTGGCCAGAGCCCGTTGATGCAGGTTCCCGGATCACAACTTATGGGAAGATCAGCTGCATCTCCTGGTGGCACAAGTATTACAAACTTCGATAACACAACAACATCTCCATTACCATATGCCAACTCTCCAAGGTCTAGTACAAACATTATGAATACGCCATCTCCTCAACAACAAAGCCAGCAACAGAAGCAGCAACAACAGCAGCAACAGCAGCAGCAGCAGCAGCAGAAGTTGATGCAATTACCTCCTCAACAGCAACTCCTGGCTCAGCAACAATTTAGGCAATCGGGAATGCAAGGACTTGGACAAAACCCACTGCATCAGCTGCATGATTTGCAGGGACAGGCGCAGCAAAAGTTCCAGTCGGTAAATTTGTTGCCTAGCTTCTTTTTTCTTGTACTTCTTTTTTTCAGGAGCCTGTTGGCCATGAACCTGTTATCCATGCCTGTTATACTCTATCTCCCTCCCTCTCACTCCACCACTTTCCTTTATTCTTATAGTTACAGGCATTTGACTGAGATATACTTTTGCATCAACAATCAAAAGAACTTTTTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

457

Amino Acids

50.74

Weight (kDa)

8.78

Isoelectric Point (pI)

63.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 694
Acc36I ACCTGC 2 cut(s) 694, 823
AccI GTMKAC 1 cut(s) 270
AccII CGCG 2 cut(s) 20, 71
AciI CCGC 6 cut(s) 62, 69, 71, 74, 163, 723
AclWI GGATC 1 cut(s) 850
AcoI YGGCCR 3 cut(s) 184, 814, 1236
AcsI RAATTY 2 cut(s) 600, 1183
AcuI CTGAAG 1 cut(s) 780
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 5 cut(s) 99, 236, 776, 952, 1212
AgsI TTSAA 3 cut(s) 151, 262, 605
AjnI CCWGG 3 cut(s) 249, 874, 1074
AloI GAACNNNNNNTCC 4 cut(s) 104, 136, 1235, 1267
Alw21I GWGCWC 2 cut(s) 258, 717
Alw26I GTCTC 2 cut(s) 76, 292
AlwI GGATC 1 cut(s) 850
AlwNI CAGNNNCTG 1 cut(s) 109
AoxI GGCC 4 cut(s) 184, 294, 814, 1236
ApoI RAATTY 2 cut(s) 600, 1183
AspLEI GCGC 3 cut(s) 20, 22, 1162
AspS9I GGNCC 2 cut(s) 130, 220
AsuC2I CCSGG 2 cut(s) 626, 840
AsuHPI GGTGA 3 cut(s) 151, 361, 746
AvaII GGWCC 2 cut(s) 130, 220
BaeI ACNNNNGTAYC 2 cut(s) 777, 810
BalI TGGCCA 2 cut(s) 816, 1238
BanII GRGCYC 2 cut(s) 258, 824
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 2 cut(s) 258, 717
BccI CCATC 3 cut(s) 373, 731, 979
BceAI ACGGC 3 cut(s) 80, 199, 320
BcgI CGANNNNNNTGC 4 cut(s) 352, 377, 386, 411
BciT130I CCWGG 3 cut(s) 251, 876, 1076
BcnI CCSGG 2 cut(s) 626, 840
BcoDI GTCTC 2 cut(s) 76, 292
BfaI CTAG 4 cut(s) 419, 468, 948, 1194
BfmI CTRYAG 1 cut(s) 23
BfuAI ACCTGC 2 cut(s) 694, 823
BlpI GCTNAGC 1 cut(s) 1080
BmcAI AGTACT 1 cut(s) 236
Bme1390I CCNGG 5 cut(s) 251, 626, 840, 876, 1076
Bme18I GGWCC 2 cut(s) 130, 220
BmgT120I GGNCC 2 cut(s) 130, 220
BmiI GGNNCC 2 cut(s) 837, 1229
BmrFI CCNGG 5 cut(s) 251, 626, 840, 876, 1076
BmsI GCATC 7 cut(s) 342, 709, 819, 878, 1038, 1141, 1353
BpiI GAAGAC 1 cut(s) 180
BpmI CTGGAG 2 cut(s) 272, 648
Bpu10I CCTNAGC 2 cut(s) 227, 588
Bpu1102I GCTNAGC 1 cut(s) 1080
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 2 cut(s) 626, 840
BsaHI GRCGYC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 292
BsaJI CCNNGG 6 cut(s) 9, 69, 240, 291, 336, 941
BsaWI WCCGGW 1 cut(s) 562
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 2 cut(s) 232, 1174
BseBI CCWGG 3 cut(s) 251, 876, 1076
BseDI CCNNGG 6 cut(s) 9, 69, 240, 291, 336, 941
BseMII CTCAG 4 cut(s) 218, 602, 1094, 1320
BseNI ACTGG 2 cut(s) 232, 1174
BsePI GCGCGC 1 cut(s) 18
BseRI GAGGAG 2 cut(s) 966, 1050
BsgI GTGCAG 1 cut(s) 672
Bsh1236I CGCG 2 cut(s) 20, 71
BshFI GGCC 4 cut(s) 186, 296, 816, 1238
BsiHKAI GWGCWC 2 cut(s) 258, 717
BsiSI CCGG 5 cut(s) 347, 374, 563, 625, 840
BslFI GGGAC 2 cut(s) 659, 1167
BsmAI GTCTC 2 cut(s) 76, 292
BsmFI GGGAC 2 cut(s) 659, 1167
BsmI GAATGC 3 cut(s) 165, 558, 1110
BsnI GGCC 4 cut(s) 186, 296, 816, 1238
Bso31I GGTCTC 1 cut(s) 292
Bsp1286I GDGCHC 3 cut(s) 258, 717, 824
Bsp143I GATC 2 cut(s) 842, 860
Bsp1720I GCTNAGC 1 cut(s) 1080
Bsp19I CCATGG 2 cut(s) 9, 240
BspACI CCGC 6 cut(s) 62, 69, 71, 74, 163, 723
BspANI GGCC 4 cut(s) 186, 296, 816, 1238
BspCNI CTCAG 4 cut(s) 219, 601, 1093, 1321
BspFNI CGCG 2 cut(s) 20, 71
BspLI GGNNCC 2 cut(s) 837, 1229
BspMI ACCTGC 2 cut(s) 694, 823
BspPI GGATC 1 cut(s) 850
BspQI GCTCTTC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 2 cut(s) 232, 1174
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 6 cut(s) 9, 69, 240, 291, 336, 941
BssHII GCGCGC 1 cut(s) 18
BssMI GATC 2 cut(s) 842, 860
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 4 cut(s) 9, 240, 291, 941
Bst2UI CCWGG 3 cut(s) 251, 876, 1076
Bst4CI ACNGT 1 cut(s) 657
Bst6I CTCTTC 1 cut(s) 263
BstACI GRCGYC 1 cut(s) 93
BstAPI GCANNNNNTGC 2 cut(s) 1138, 1147
BstC8I GCNNGC 4 cut(s) 20, 193, 750, 992
BstDEI CTNAG 5 cut(s) 227, 399, 588, 1080, 1329
BstDSI CCRYGG 4 cut(s) 9, 69, 240, 336
BstEII GGTNACC 1 cut(s) 657
BstFNI CGCG 2 cut(s) 20, 71
BstHHI GCGC 3 cut(s) 20, 22, 1162
BstKTI GATC 2 cut(s) 845, 863
BstMAI GTCTC 2 cut(s) 76, 292
BstMBI GATC 2 cut(s) 842, 860
BstNI CCWGG 3 cut(s) 251, 876, 1076
BstPI GGTNACC 1 cut(s) 657
BstSCI CCNGG 5 cut(s) 249, 624, 838, 874, 1074
BstSFI CTRYAG 1 cut(s) 23
BstUI CGCG 2 cut(s) 20, 71
BstV2I GAAGAC 1 cut(s) 180
BsuRI GGCC 4 cut(s) 186, 296, 816, 1238
BtgI CCRYGG 4 cut(s) 9, 69, 240, 336
BtsI GCAGTG 1 cut(s) 1127
BtsIMutI CAGTG 1 cut(s) 1127
BveI ACCTGC 2 cut(s) 694, 823
Cac8I GCNNGC 4 cut(s) 20, 193, 750, 992
CaiI CAGNNNCTG 1 cut(s) 109
CfoI GCGC 3 cut(s) 20, 22, 1162
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 2 cut(s) 130, 220
Cfr42I CCGCGG 1 cut(s) 72
CseI GACGC 3 cut(s) 101, 656, 753
Csp6I GTAC 5 cut(s) 98, 235, 775, 951, 1211
CviAII CATG 5 cut(s) 10, 241, 1142, 1240, 1255
CviQI GTAC 5 cut(s) 98, 235, 775, 951, 1211
DdeI CTNAG 5 cut(s) 227, 399, 588, 1080, 1329
DpnI GATC 2 cut(s) 844, 862
DpnII GATC 2 cut(s) 842, 860
EaeI YGGCCR 3 cut(s) 184, 814, 1236
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 4 cut(s) 9, 240, 291, 941
Eco147I AGGCCT 1 cut(s) 296
Eco24I GRGCYC 2 cut(s) 258, 824
Eco31I GGTCTC 1 cut(s) 292
Eco47I GGWCC 2 cut(s) 130, 220
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 780
Eco91I GGTNACC 1 cut(s) 657
EcoICRI GAGCTC 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 220
EcoO65I GGTNACC 1 cut(s) 657
EcoRII CCWGG 3 cut(s) 249, 874, 1074
EcoT14I CCWWGG 4 cut(s) 9, 240, 291, 941
EcoT22I ATGCAT 1 cut(s) 702
EcoT38I GRGCYC 2 cut(s) 258, 824
ErhI CCWWGG 4 cut(s) 9, 240, 291, 941
FaeI CATG 5 cut(s) 13, 244, 1145, 1243, 1258
FaqI GGGAC 2 cut(s) 659, 1167
FatI CATG 5 cut(s) 9, 240, 1141, 1239, 1254
FauI CCCGC 2 cut(s) 76, 170
FauNDI CATATG 1 cut(s) 928
FblI GTMKAC 1 cut(s) 270
FriOI GRGCYC 2 cut(s) 258, 824
FspBI CTAG 4 cut(s) 419, 468, 948, 1194
GlaI GCGC 3 cut(s) 19, 21, 1161
GsuI CTGGAG 2 cut(s) 272, 648
HaeIII GGCC 4 cut(s) 186, 296, 816, 1238
HapII CCGG 5 cut(s) 347, 374, 563, 625, 840
HgaI GACGC 3 cut(s) 101, 656, 753
HhaI GCGC 3 cut(s) 20, 22, 1162
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 5 cut(s) 13, 244, 1145, 1243, 1258
Hin6I GCGC 3 cut(s) 18, 20, 1160
HinP1I GCGC 3 cut(s) 18, 20, 1160
HincII GTYRAC 2 cut(s) 247, 271
HindII GTYRAC 2 cut(s) 247, 271
HinfI GANTC 2 cut(s) 616, 679
HpaII CCGG 5 cut(s) 347, 374, 563, 625, 840
HphI GGTGA 3 cut(s) 151, 361, 746
Hpy166II GTNNAC 3 cut(s) 247, 271, 322
Hpy188I TCNGA 1 cut(s) 528
Hpy188III TCNNGA 9 cut(s) 170, 226, 442, 578, 605, 665, 758, 1101, 1225
Hpy8I GTNNAC 3 cut(s) 247, 271, 322
Hpy99I CGWCG 5 cut(s) 95, 185, 272, 275, 364
HpyAV CCTTC 2 cut(s) 8, 307
HpyCH4III ACNGT 1 cut(s) 657
HpyF3I CTNAG 5 cut(s) 227, 399, 588, 1080, 1329
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 5 cut(s) 13, 244, 1145, 1243, 1258
HspAI GCGC 3 cut(s) 18, 20, 1160
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 2 cut(s) 842, 860
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 3 cut(s) 253, 485, 1227
LweI GCATC 7 cut(s) 342, 709, 819, 878, 1038, 1141, 1353
MaeI CTAG 4 cut(s) 419, 468, 948, 1194
MaeIII GTNAC 3 cut(s) 657, 803, 1313
MalI GATC 2 cut(s) 844, 862
MboI GATC 2 cut(s) 842, 860
MboII GAAGA 3 cut(s) 185, 250, 870
MhlI GDGCHC 3 cut(s) 258, 717, 824
MlsI TGGCCA 2 cut(s) 816, 1238
MluCI AATT 6 cut(s) 450, 600, 637, 1052, 1088, 1183
MluNI TGGCCA 2 cut(s) 816, 1238
MmeI TCCRAC 1 cut(s) 73
Mox20I TGGCCA 2 cut(s) 816, 1238
Mph1103I ATGCAT 1 cut(s) 702
MscI TGGCCA 2 cut(s) 816, 1238
MseI TTAA 1 cut(s) 636
Msp20I TGGCCA 2 cut(s) 816, 1238
MspA1I CMGCKG 4 cut(s) 71, 109, 866, 1138
MspI CCGG 5 cut(s) 347, 374, 563, 625, 840
MspR9I CCNGG 5 cut(s) 251, 626, 840, 876, 1076
Mva1269I GAATGC 3 cut(s) 165, 558, 1110
MvaI CCWGG 3 cut(s) 251, 876, 1076
MvnI CGCG 2 cut(s) 20, 71
NciI CCSGG 2 cut(s) 626, 840
NcoI CCATGG 2 cut(s) 9, 240
NdeI CATATG 1 cut(s) 928
NdeII GATC 2 cut(s) 842, 860
NlaIII CATG 5 cut(s) 13, 244, 1145, 1243, 1258
NlaIV GGNNCC 2 cut(s) 837, 1229
NmeAIII GCCGAG 3 cut(s) 352, 529, 529
NsiI ATGCAT 1 cut(s) 702
PaqCI CACCTGC 1 cut(s) 694
PauI GCGCGC 1 cut(s) 18
PceI AGGCCT 1 cut(s) 296
PciSI GCTCTTC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 3 cut(s) 165, 558, 1110
PfeI GAWTC 2 cut(s) 616, 679
PfoI TCCNGGA 1 cut(s) 838
PpuMI RGGWCCY 1 cut(s) 220
Psp124BI GAGCTC 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 3 cut(s) 249, 874, 1074
PspEI GGTNACC 1 cut(s) 657
PspGI CCWGG 3 cut(s) 249, 874, 1074
PspN4I GGNNCC 2 cut(s) 837, 1229
PspPI GGNCC 2 cut(s) 130, 220
PspPPI RGGWCCY 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 109
PteI GCGCGC 1 cut(s) 18
PvuII CAGCTG 3 cut(s) 109, 866, 1138
RsaI GTAC 5 cut(s) 99, 236, 776, 952, 1212
RsaNI GTAC 5 cut(s) 98, 235, 775, 951, 1211
SacI GAGCTC 1 cut(s) 258
SacII CCGCGG 1 cut(s) 72
SalI GTCGAC 1 cut(s) 269
SapI GCTCTTC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 636
Sau3AI GATC 2 cut(s) 842, 860
Sau96I GGNCC 2 cut(s) 130, 220
ScaI AGTACT 1 cut(s) 236
ScrFI CCNGG 5 cut(s) 251, 626, 840, 876, 1076
SduI GDGCHC 3 cut(s) 258, 717, 824
SfaNI GCATC 7 cut(s) 342, 709, 819, 878, 1038, 1141, 1353
SfcI CTRYAG 1 cut(s) 23
Sfr303I CCGCGG 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 72
SgrDI CGTCGACG 1 cut(s) 269
SinI GGWCC 2 cut(s) 130, 220
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 6 cut(s) 450, 600, 637, 1052, 1088, 1183
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 6 cut(s) 62, 69, 71, 74, 163, 723
SspMI CTAG 4 cut(s) 419, 468, 948, 1194
SstI GAGCTC 1 cut(s) 258
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 5 cut(s) 249, 624, 838, 874, 1074
StyI CCWWGG 4 cut(s) 9, 240, 291, 941
TaaI ACNGT 1 cut(s) 657
TaqI TCGA 7 cut(s) 171, 180, 270, 362, 387, 443, 900
TaqII GACCGA 1 cut(s) 147
TasI AATT 6 cut(s) 450, 600, 637, 1052, 1088, 1183
TatI WGTACW 4 cut(s) 234, 774, 950, 1210
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 2 cut(s) 616, 679
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 1 cut(s) 1134
TspDTI ATGAA 2 cut(s) 977, 1256
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 1 cut(s) 1134
VpaK11BI GGWCC 2 cut(s) 130, 220
XapI RAATTY 2 cut(s) 600, 1183
XcmI CCANNNNNNNNNTGG 2 cut(s) 247, 298
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 4 cut(s) 419, 468, 948, 1194
ZrmI AGTACT 1 cut(s) 236
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.