Rh4CG183200

Peptidyl-prolyl

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014948
Physical Location & Seq
Reverse (-)
39489895 .. 39493516
3622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG183200.1

Sequence Viewer

Length: 1323 bp
ATGGAAGACGACTTTGATTTCCCAACCGGCGGCAACCCCGAAGACGTTGACATGGACCTCCCGGAGGAAGACGACGACGCGACCAGCCCAGGCCCGCTCAAGGTCGGCGAAGAGAAGGAGATTGGGAAAAATGGGCTGCAGAAGAAGCTGCTCAAGCAAGGTGAAGGGTGGGAGACGCCTGGCTCCGGCGACGAGGTTGAAGTTCATTACACTGGGACTTTGCTTGATGGCACCAAGTTTGATTCCAGCCGTGATCGGGAGACGCCTTTCAAGTTCGGCCTCGGACAAGGACAAGTGATCAAGGGATGGGATGAGGGTATTAAGACCATGAAGAAGGGTGAAAATGCAATTTTCACCATACCGCCTGAGTTGGCTTATGGCGAGTCTGGATCCCCTCCAACTATTCCTCCCAATGCCACACTCCAGTTTGATGTGGAGTTGCTTTCCTGGACTAGTGTCAAGGACATATGCAAGGATGGCGGTGTTATAAAGAAAATTCTTACTGAAGGGGAGAAATGGGAGAATCCAAAAGACCTCGATGAAGTATTTGTTAAGTATGAGGCTCGACTTGAAGATGGGACTCTTGTTTCAAAATCTGATGGGGTGGAGTTTACTGTTGAAGATGGCTATTTCTGCCCAGCCTTGGCAAAAGCTGTGAAAACAATGAAGAAAAATGAAAAGGTCCATTTGACAGTAAAACCACAATATGGATTTCGTGAGGCCGGAAGACCAGCTGCTGGTGAAGAAGGCGCAGTGCCTCCAAATGCTACCCTTGGGATAACCCTAGAGTTGGTCTCGTGGAAGACAGTTTCAGATGTTACAAAGGACAAGAAGGTCCTAAAAAAGGCCTTAAAGGAGGGAGAGGGATATGAGCGTCCTAATGATGGGGCGGTAGTTCAAGTGAAACTTGTTGGGAAGTTACAGGATGGCACAATCTTTATGAAGAAGGGACATGATAATGAGCCATTTGAATTCAAGATAGATGAAGAGCAAGTTATTGATGGACTTGATAGAGCGGTGAAGGGCATGAAGAAAGGGGAGGTGGCTCTGGTCACCATTCAACCGGAATATGCTTTTGGGTCATCTGATTCTGCACAAGAATTGGCCATTGTCCCTGCAAATTCAACTGTGTACTATGAGGTGGAGCTGGTCTCCTTTGTGAAGGAGAAGGAATCTTGGGACATGAACACGCAAGAAAAAATTGAAGCGGCTAGTAAGAAGAAGGAAGAAGGCAACGCTCTCTTTAAGGCTGCCAAATATGAAAGGGCCTCAAAGAGATATGAAAAGGTATGTATGCTGAATGTTGTCTACCGGTTCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04915 map04915
Pfam Domains
Protein Families
FKBP_C TPR_1 TPR_8

Protein Analysis

440

Amino Acids

48.76

Weight (kDa)

4.86

Isoelectric Point (pI)

25.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKBP_C PF00254 57 - 148 7e-38 FKBP-type peptidyl-prolyl cis-trans isomerase
FKBP_C PF00254 174 - 264 2.3e-16 FKBP-type peptidyl-prolyl cis-trans isomerase
FKBP_C PF00254 290 - 384 9.7e-26 FKBP-type peptidyl-prolyl cis-trans isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014948)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48570
fragaria_vesca FvH4_4g12830
malus_domestica MD04G1022400.v1.1
prunus_persica Prupe.1G172600_v2.0.a1
pyrus_communis pycom04g01940
rosa_chinensis RchiOBHm_Chr4g0412331
rosa_laevigata RLG00000008307
rosa_multiflora Rmu_co8161348.1_g000001 Rmu_sc0010364.1_g000002 Rmu_sc0024446.1_g000002 Rmu_sc0041848.1_g000001
rosa_roxburghii Rroxscaffold_5G00356460
rosa_rugosa Rorug04G0104700
rosa_samantha Rh4AG172300 Rh4CG183200
rosa_wichuraiana Rw4G014400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 488
AasI GACNNNNNNGTC 1 cut(s) 833
AccB1I GGYRCC 1 cut(s) 230
AccB7I CCANNNNNTGG 2 cut(s) 707, 737
AccBSI CCGCTC 2 cut(s) 97, 1016
AccI GTMKAC 1 cut(s) 1308
AccII CGCG 1 cut(s) 80
AciI CCGC 7 cut(s) 30, 95, 362, 480, 890, 1016, 1208
AclWI GGATC 2 cut(s) 384, 397
AcoI YGGCCR 1 cut(s) 1104
AcsI RAATTY 3 cut(s) 495, 971, 1120
AcuI CTGAAG 1 cut(s) 525
AcyI GRCGYC 2 cut(s) 176, 263
AfaI GTAC 1 cut(s) 1133
AgeI ACCGGT 1 cut(s) 1311
AhlI ACTAGT 1 cut(s) 452
AjnI CCWGG 3 cut(s) 88, 178, 446
AjuI GAANNNNNNNTTGG 2 cut(s) 1059, 1091
AluBI AGCT 4 cut(s) 148, 653, 734, 1147
AluI AGCT 4 cut(s) 148, 653, 734, 1147
Alw26I GTCTC 4 cut(s) 167, 254, 799, 1156
AlwI GGATC 2 cut(s) 384, 397
AlwNI CAGNNNCTG 1 cut(s) 737
AoxI GGCC 6 cut(s) 91, 277, 720, 846, 1104, 1266
ApeKI GCWGC 4 cut(s) 136, 148, 734, 1250
ApoI RAATTY 3 cut(s) 495, 971, 1120
AsiGI ACCGGT 1 cut(s) 1311
AspLEI GCGC 1 cut(s) 752
AspS9I GGNCC 5 cut(s) 55, 92, 682, 835, 1266
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 6 cut(s) 173, 346, 350, 752, 1030, 1045
AvaII GGWCC 3 cut(s) 55, 682, 835
BalI TGGCCA 1 cut(s) 1106
BamHI GGATCC 1 cut(s) 389
BanI GGYRCC 1 cut(s) 230
BauI CACGAG 1 cut(s) 796
BbsI GAAGAC 5 cut(s) 12, 48, 75, 733, 809
BbvI GCAGC 4 cut(s) 123, 135, 721, 1237
BccI CCATC 9 cut(s) 221, 300, 470, 569, 593, 617, 878, 920, 995
BceAI ACGGC 1 cut(s) 234
BciT130I CCWGG 3 cut(s) 90, 180, 448
BclI TGATCA 1 cut(s) 297
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 4 cut(s) 167, 254, 799, 1156
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 3 cut(s) 453, 785, 1212
BfmI CTRYAG 1 cut(s) 137
BisI GCNGC 6 cut(s) 31, 137, 149, 735, 1209, 1251
BlsI GCNGC 6 cut(s) 32, 138, 150, 736, 1210, 1252
Bme1390I CCNGG 4 cut(s) 62, 90, 180, 448
Bme18I GGWCC 3 cut(s) 55, 682, 835
BmgT120I GGNCC 5 cut(s) 55, 92, 682, 835, 1266
BmiI GGNNCC 3 cut(s) 184, 232, 391
BmrFI CCNGG 4 cut(s) 62, 90, 180, 448
BmrI ACTGGG 1 cut(s) 222
BmuI ACTGGG 1 cut(s) 222
BoxI GACNNNNGTC 1 cut(s) 455
BpiI GAAGAC 5 cut(s) 12, 48, 75, 733, 809
BplI GAGNNNNNCTC 4 cut(s) 779, 811, 1136, 1168
BpmI CTGGAG 1 cut(s) 407
BpuEI CTTGAG 2 cut(s) 83, 137
BpuMI CCSGG 1 cut(s) 62
BsaHI GRCGYC 2 cut(s) 176, 263
BsaI GGTCTC 2 cut(s) 799, 1156
BsaJI CCNNGG 4 cut(s) 88, 280, 642, 772
BsaWI WCCGGW 2 cut(s) 1063, 1311
BsaXI ACNNNNNCTCC 2 cut(s) 391, 421
Bse118I RCCGGY 2 cut(s) 26, 1311
Bse1I ACTGG 2 cut(s) 217, 424
BseBI CCWGG 3 cut(s) 90, 180, 448
BseDI CCNNGG 4 cut(s) 88, 280, 642, 772
BseGI GGATG 4 cut(s) 311, 316, 481, 931
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 2 cut(s) 217, 424
BseXI GCAGC 4 cut(s) 123, 135, 721, 1237
BseYI CCCAGC 1 cut(s) 637
BsgI GTGCAG 1 cut(s) 1077
Bsh1236I CGCG 1 cut(s) 80
BshFI GGCC 6 cut(s) 93, 279, 722, 848, 1106, 1268
BshNI GGYRCC 1 cut(s) 230
BshTI ACCGGT 1 cut(s) 1311
BsiSI CCGG 6 cut(s) 27, 62, 186, 723, 1064, 1312
BslFI GGGAC 5 cut(s) 229, 592, 963, 1097, 1193
BsmAI GTCTC 4 cut(s) 167, 254, 799, 1156
BsmBI CGTCTC 2 cut(s) 167, 254
BsmFI GGGAC 5 cut(s) 229, 592, 963, 1097, 1193
BsnI GGCC 6 cut(s) 93, 279, 722, 848, 1106, 1268
Bso31I GGTCTC 2 cut(s) 799, 1156
Bsp143I GATC 3 cut(s) 253, 297, 389
BspACI CCGC 7 cut(s) 30, 95, 362, 480, 890, 1016, 1208
BspANI GGCC 6 cut(s) 93, 279, 722, 848, 1106, 1268
BspCNI CTCAG 1 cut(s) 358
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 3 cut(s) 184, 232, 391
BspMAI CTGCAG 1 cut(s) 141
BspPI GGATC 2 cut(s) 384, 397
BspQI GCTCTTC 1 cut(s) 981
BspT107I GGYRCC 1 cut(s) 230
BspTNI GGTCTC 2 cut(s) 799, 1156
BsrBI CCGCTC 2 cut(s) 97, 1016
BsrFI RCCGGY 2 cut(s) 26, 1311
BsrI ACTGG 2 cut(s) 217, 424
BssAI RCCGGY 2 cut(s) 26, 1311
BssECI CCNNGG 4 cut(s) 88, 280, 642, 772
BssMI GATC 3 cut(s) 253, 297, 389
BssNI GRCGYC 2 cut(s) 176, 263
BssSI CACGAG 1 cut(s) 796
BssT1I CCWWGG 2 cut(s) 642, 772
Bst2BI CACGAG 1 cut(s) 796
Bst2UI CCWGG 3 cut(s) 90, 180, 448
Bst4CI ACNGT 4 cut(s) 616, 694, 808, 1129
Bst6I CTCTTC 2 cut(s) 105, 981
BstACI GRCGYC 2 cut(s) 176, 263
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 366
BstEII GGTNACC 1 cut(s) 1051
BstENI CCTNNNNNAGG 2 cut(s) 62, 842
BstF5I GGATG 4 cut(s) 311, 316, 481, 931
BstFNI CGCG 1 cut(s) 80
BstHHI GCGC 1 cut(s) 752
BstKTI GATC 3 cut(s) 256, 300, 392
BstMAI GTCTC 4 cut(s) 167, 254, 799, 1156
BstMBI GATC 3 cut(s) 253, 297, 389
BstMWI GCNNNNNNNGC 4 cut(s) 145, 154, 477, 633
BstNI CCWGG 3 cut(s) 90, 180, 448
BstPAI GACNNNNGTC 1 cut(s) 455
BstPI GGTNACC 1 cut(s) 1051
BstSCI CCNGG 4 cut(s) 60, 88, 178, 446
BstSFI CTRYAG 1 cut(s) 137
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 4 cut(s) 123, 135, 721, 1237
BstV2I GAAGAC 5 cut(s) 12, 48, 75, 733, 809
BstX2I RGATCY 1 cut(s) 389
BstYI RGATCY 1 cut(s) 389
BsuRI GGCC 6 cut(s) 93, 279, 722, 848, 1106, 1268
BtsCI GGATG 4 cut(s) 311, 316, 481, 931
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 2 cut(s) 210, 759
Cac8I GCNNGC 1 cut(s) 95
CaiI CAGNNNCTG 1 cut(s) 737
CfoI GCGC 1 cut(s) 752
Cfr10I RCCGGY 2 cut(s) 26, 1311
Cfr13I GGNCC 5 cut(s) 55, 92, 682, 835, 1266
CseI GACGC 4 cut(s) 86, 184, 271, 863
Csp6I GTAC 1 cut(s) 1132
CspAI ACCGGT 1 cut(s) 1311
CviAII CATG 5 cut(s) 52, 328, 953, 1027, 1183
CviQI GTAC 1 cut(s) 1132
DdeI CTNAG 1 cut(s) 366
DpnI GATC 3 cut(s) 255, 299, 391
DpnII GATC 3 cut(s) 253, 297, 389
DrdI GACNNNNNNGTC 1 cut(s) 833
DseDI GACNNNNNNGTC 1 cut(s) 833
EaeI YGGCCR 1 cut(s) 1104
Eam1104I CTCTTC 2 cut(s) 105, 981
EarI CTCTTC 2 cut(s) 105, 981
Eco130I CCWWGG 2 cut(s) 642, 772
Eco147I AGGCCT 1 cut(s) 848
Eco31I GGTCTC 2 cut(s) 799, 1156
Eco47I GGWCC 3 cut(s) 55, 682, 835
Eco57I CTGAAG 1 cut(s) 525
Eco91I GGTNACC 1 cut(s) 1051
EcoNI CCTNNNNNAGG 2 cut(s) 62, 842
EcoO109I RGGNCCY 2 cut(s) 835, 1266
EcoO65I GGTNACC 1 cut(s) 1051
EcoRI GAATTC 1 cut(s) 971
EcoRII CCWGG 3 cut(s) 88, 178, 446
EcoT14I CCWWGG 2 cut(s) 642, 772
ErhI CCWWGG 2 cut(s) 642, 772
Esp3I CGTCTC 2 cut(s) 167, 254
FaeI CATG 5 cut(s) 55, 331, 956, 1030, 1186
FalI AAGNNNNNCTT 2 cut(s) 891, 923
FaqI GGGAC 5 cut(s) 229, 592, 963, 1097, 1193
FatI CATG 5 cut(s) 51, 327, 952, 1026, 1182
FauI CCCGC 1 cut(s) 102
FauNDI CATATG 1 cut(s) 467
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 1 cut(s) 1308
Fnu4HI GCNGC 6 cut(s) 31, 137, 149, 735, 1209, 1251
FokI GGATG 4 cut(s) 318, 323, 488, 938
Fsp4HI GCNGC 6 cut(s) 31, 137, 149, 735, 1209, 1251
FspBI CTAG 3 cut(s) 453, 785, 1212
GlaI GCGC 1 cut(s) 751
GluI GCNGC 6 cut(s) 31, 137, 149, 735, 1209, 1251
GsaI CCCAGC 1 cut(s) 641
GsuI CTGGAG 1 cut(s) 407
HaeIII GGCC 6 cut(s) 93, 279, 722, 848, 1106, 1268
HapII CCGG 6 cut(s) 27, 62, 186, 723, 1064, 1312
HgaI GACGC 4 cut(s) 86, 184, 271, 863
HhaI GCGC 1 cut(s) 752
Hin1I GRCGYC 2 cut(s) 176, 263
Hin1II CATG 5 cut(s) 55, 331, 956, 1030, 1186
Hin6I GCGC 1 cut(s) 750
HinP1I GCGC 1 cut(s) 750
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 6 cut(s) 242, 383, 523, 580, 1088, 1172
HpaII CCGG 6 cut(s) 27, 62, 186, 723, 1064, 1312
HphI GGTGA 6 cut(s) 173, 346, 350, 752, 1030, 1045
Hpy166II GTNNAC 4 cut(s) 49, 612, 1132, 1309
Hpy188I TCNGA 4 cut(s) 284, 598, 814, 1087
Hpy188III TCNNGA 4 cut(s) 257, 387, 716, 976
Hpy8I GTNNAC 4 cut(s) 49, 612, 1132, 1309
Hpy99I CGWCG 3 cut(s) 77, 80, 194
HpyCH4III ACNGT 4 cut(s) 616, 694, 808, 1129
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 5 cut(s) 139, 347, 471, 1094, 1118
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 154, 477, 633
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 2 cut(s) 176, 263
Hsp92II CATG 5 cut(s) 55, 331, 956, 1030, 1186
HspAI GCGC 1 cut(s) 750
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 3 cut(s) 253, 297, 389
LguI GCTCTTC 1 cut(s) 981
LmnI GCTCC 2 cut(s) 188, 1144
Lsp1109I GCAGC 4 cut(s) 123, 135, 721, 1237
MaeI CTAG 3 cut(s) 453, 785, 1212
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 3 cut(s) 817, 918, 1051
MalI GATC 3 cut(s) 255, 299, 391
MbiI CCGCTC 2 cut(s) 97, 1016
MboI GATC 3 cut(s) 253, 297, 389
MflI RGATCY 1 cut(s) 389
MlsI TGGCCA 1 cut(s) 1106
MluCI AATT 6 cut(s) 348, 495, 971, 1100, 1120, 1200
MluNI TGGCCA 1 cut(s) 1106
MlyI GAGTC 2 cut(s) 392, 574
MmeI TCCRAC 1 cut(s) 422
Mox20I TGGCCA 1 cut(s) 1106
MscI TGGCCA 1 cut(s) 1106
MseI TTAA 4 cut(s) 321, 552, 851, 1245
MslI CAYNNNNRTG 1 cut(s) 957
Msp20I TGGCCA 1 cut(s) 1106
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 6 cut(s) 27, 62, 186, 723, 1064, 1312
MspR9I CCNGG 4 cut(s) 62, 90, 180, 448
MvaI CCWGG 3 cut(s) 90, 180, 448
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 4 cut(s) 145, 154, 477, 633
NciI CCSGG 1 cut(s) 62
NdeI CATATG 1 cut(s) 467
NdeII GATC 3 cut(s) 253, 297, 389
NlaIII CATG 5 cut(s) 55, 331, 956, 1030, 1186
NlaIV GGNNCC 3 cut(s) 184, 232, 391
NmuCI GTSAC 1 cut(s) 1051
PceI AGGCCT 1 cut(s) 848
PciSI GCTCTTC 1 cut(s) 981
PfeI GAWTC 4 cut(s) 242, 523, 1088, 1172
PflMI CCANNNNNTGG 2 cut(s) 707, 737
PfoI TCCNGGA 2 cut(s) 60, 446
PinAI ACCGGT 1 cut(s) 1311
PkrI GCNGC 6 cut(s) 32, 138, 150, 736, 1210, 1252
PleI GAGTC 2 cut(s) 391, 574
PpsI GAGTC 2 cut(s) 391, 574
PpuMI RGGWCCY 1 cut(s) 835
PshAI GACNNNNGTC 1 cut(s) 455
PsiI TTATAA 1 cut(s) 488
Psp5II RGGWCCY 1 cut(s) 835
Psp6I CCWGG 3 cut(s) 88, 178, 446
PspEI GGTNACC 1 cut(s) 1051
PspFI CCCAGC 1 cut(s) 637
PspGI CCWGG 3 cut(s) 88, 178, 446
PspN4I GGNNCC 3 cut(s) 184, 232, 391
PspPI GGNCC 5 cut(s) 55, 92, 682, 835, 1266
PspPPI RGGWCCY 1 cut(s) 835
PstI CTGCAG 1 cut(s) 141
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 1 cut(s) 389
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 1 cut(s) 1133
RsaNI GTAC 1 cut(s) 1132
RseI CAYNNNNRTG 1 cut(s) 957
SapI GCTCTTC 1 cut(s) 981
SaqAI TTAA 4 cut(s) 321, 552, 851, 1245
SatI GCNGC 6 cut(s) 31, 137, 149, 735, 1209, 1251
Sau3AI GATC 3 cut(s) 253, 297, 389
Sau96I GGNCC 5 cut(s) 55, 92, 682, 835, 1266
SchI GAGTC 2 cut(s) 392, 574
ScrFI CCNGG 4 cut(s) 62, 90, 180, 448
SfcI CTRYAG 1 cut(s) 137
SinI GGWCC 3 cut(s) 55, 682, 835
SmiMI CAYNNNNRTG 1 cut(s) 957
SmlI CTYRAG 2 cut(s) 98, 152
SmoI CTYRAG 2 cut(s) 98, 152
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 6 cut(s) 348, 495, 971, 1100, 1120, 1200
SseBI AGGCCT 1 cut(s) 848
SsiI CCGC 7 cut(s) 30, 95, 362, 480, 890, 1016, 1208
SspMI CTAG 3 cut(s) 453, 785, 1212
StuI AGGCCT 1 cut(s) 848
StyD4I CCNGG 4 cut(s) 60, 88, 178, 446
StyI CCWWGG 2 cut(s) 642, 772
TaaI ACNGT 4 cut(s) 616, 694, 808, 1129
TaiI ACGT 1 cut(s) 48
TaqI TCGA 2 cut(s) 537, 565
TasI AATT 6 cut(s) 348, 495, 971, 1100, 1120, 1200
TatI WGTACW 1 cut(s) 1131
TauI GCSGC 2 cut(s) 33, 1211
TfiI GAWTC 4 cut(s) 242, 523, 1088, 1172
Tru1I TTAA 4 cut(s) 321, 552, 851, 1245
Tru9I TTAA 4 cut(s) 321, 552, 851, 1245
TscAI CASTG 2 cut(s) 217, 759
TseFI GTSAC 1 cut(s) 1051
TseI GCWGC 4 cut(s) 136, 148, 734, 1250
Tsp45I GTSAC 1 cut(s) 1051
TspRI CASTG 2 cut(s) 217, 759
Van91I CCANNNNNTGG 2 cut(s) 707, 737
VpaK11BI GGWCC 3 cut(s) 55, 682, 835
XagI CCTNNNNNAGG 2 cut(s) 62, 842
XapI RAATTY 3 cut(s) 495, 971, 1120
XmiI GTMKAC 1 cut(s) 1308
XspI CTAG 3 cut(s) 453, 785, 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.