Rh4CG222600

protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008699
Physical Location & Seq
Reverse (-)
45906390 .. 45909850
3461 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG222600.1

Sequence Viewer

Length: 909 bp
ATGCTGGAAAAACTGCGGGACAAGCGAGTAGTGTTTGTTGGTGATTCCATTGGACGAAACCAGTGGGAATCATTCCTATGCATGCTGTCTTCGGCAGTTCCTAATAAGGATTCAATCTATGAAGTGAATGGCAACCCAATTACCAAGCACAAGGGGTTTTTGGTGTTCAAATTTAAGGACTATAACTGCACTGTGGAGTACTACAGAGCTCCATTTCTTGTAGTGCAGAGCCGTCCTCCTTCAGGAGCTTCACAAAAGATCCGAACAACTTTGAGATTGGACCAAATGGATTGGAATTCTGGAAAGTGGAGAGATGCAGATATGCTGGTTTTCAACACAGGACACTGGTGGAATTATGAGAAGACAATAAGAGGGGGTTGTTACTTCCAGGAAGGGGCAGAAGTAAAGATGGAGATGAGTGTAGAAGATGCGTATCACAGGTCCATAGAGACTGTGGTGCGATGGATAGATACTGCAGTAGATCCAAACAGGACTCAGGTTTTCTTTCGAACATATGCTCCTGTGCATTTCAGAGGTGGTGATTGGAGGAATGGTGGAGCCTGTCACCTGGAAACATTGCCTGAGCTAGCCTCTTCATTGGTGCCTACTGACTCTTGGAGTCAGTACAGGATAGCCAATGCTGTGCTATCAGCTTTGTCAAATACATCAAAGGCAATGAAGTTCAATGTGTTAAATGTAACGCAATTGACTGCTCGGAGAAAAGATGGACATTCATCTTTGTACTATCTGGGTCCTAAACTTAGCCCTGCCTCTATTCGCCGGCAAGATTGCAGCCACTGGTGTCTGCCTGGAGTCCCAGATACATGGAATGAACTACTCTATGCACTGTTTTTAAAGCATGAGAGGACTAGTACAGTGAATGCATCAACTTATAGCGCGCCGGTATGA

Protein Analysis

302

Amino Acids

34.6

Weight (kDa)

9.04

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 1 - 282 1.4e-98 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 601
AccII CGCG 1 cut(s) 899
AciI CCGC 1 cut(s) 16
AclWI GGATC 2 cut(s) 253, 476
AcsI RAATTY 2 cut(s) 170, 295
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 4 cut(s) 200, 626, 743, 874
AfiI CCNNNNNNNGG 2 cut(s) 242, 394
AgsI TTSAA 4 cut(s) 114, 169, 334, 685
AhlI ACTAGT 1 cut(s) 869
AjnI CCWGG 3 cut(s) 387, 567, 808
AloI GAACNNNNNNTCC 2 cut(s) 502, 534
AluBI AGCT 4 cut(s) 209, 248, 586, 653
AluI AGCT 4 cut(s) 209, 248, 586, 653
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 2 cut(s) 253, 476
AlwNI CAGNNNCTG 1 cut(s) 798
ApeKI GCWGC 1 cut(s) 792
ApoI RAATTY 2 cut(s) 170, 295
AspLEI GCGC 2 cut(s) 899, 901
AspS9I GGNCC 3 cut(s) 280, 441, 752
AsuHPI GGTGA 3 cut(s) 53, 551, 557
AsuII TTCGAA 1 cut(s) 508
AsuNHI GCTAGC 1 cut(s) 586
AvaII GGWCC 3 cut(s) 280, 441, 752
BaeI ACNNNNGTAYC 2 cut(s) 462, 495
BanI GGYRCC 1 cut(s) 601
BanII GRGCYC 1 cut(s) 211
BbsI GAAGAC 2 cut(s) 81, 368
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 1 cut(s) 804
BccI CCATC 3 cut(s) 403, 456, 719
BceAI ACGGC 1 cut(s) 216
BciT130I CCWGG 3 cut(s) 389, 569, 810
BcoDI GTCTC 1 cut(s) 443
BcuI ACTAGT 1 cut(s) 869
BfaI CTAG 2 cut(s) 587, 870
BfmI CTRYAG 2 cut(s) 202, 474
BisI GCNGC 1 cut(s) 793
BlsI GCNGC 1 cut(s) 794
BmcAI AGTACT 1 cut(s) 200
Bme1390I CCNGG 3 cut(s) 389, 569, 810
Bme18I GGWCC 3 cut(s) 280, 441, 752
BmgT120I GGNCC 3 cut(s) 280, 441, 752
BmiI GGNNCC 3 cut(s) 559, 603, 753
BmrFI CCNGG 3 cut(s) 389, 569, 810
BmsI GCATC 3 cut(s) 304, 418, 893
BmtI GCTAGC 1 cut(s) 590
BpiI GAAGAC 2 cut(s) 81, 368
BplI GAGNNNNNCTC 4 cut(s) 220, 252, 575, 607
BpmI CTGGAG 1 cut(s) 831
Bpu10I CCTNAGC 1 cut(s) 582
Bpu14I TTCGAA 1 cut(s) 508
BsaBI GATNNNNATC 1 cut(s) 432
BsaXI ACNNNNNCTCC 4 cut(s) 502, 532, 538, 568
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 394
Bse118I RCCGGY 2 cut(s) 780, 901
Bse1I ACTGG 3 cut(s) 61, 350, 803
Bse3DI GCAATG 2 cut(s) 575, 681
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 3 cut(s) 389, 569, 810
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 2 cut(s) 242, 394
BseMI GCAATG 2 cut(s) 575, 681
BseMII CTCAG 2 cut(s) 509, 573
BseNI ACTGG 3 cut(s) 61, 350, 803
BsePI GCGCGC 1 cut(s) 897
BseXI GCAGC 1 cut(s) 804
BsgI GTGCAG 2 cut(s) 172, 245
Bsh1236I CGCG 1 cut(s) 899
BshNI GGYRCC 1 cut(s) 601
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 2 cut(s) 781, 902
BslFI GGGAC 2 cut(s) 32, 800
BslI CCNNNNNNNGG 2 cut(s) 242, 394
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 2 cut(s) 32, 800
BsmI GAATGC 1 cut(s) 886
Bsp119I TTCGAA 1 cut(s) 508
Bsp1286I GDGCHC 1 cut(s) 211
Bsp143I GATC 2 cut(s) 258, 481
BspACI CCGC 1 cut(s) 16
BspCNI CTCAG 2 cut(s) 508, 574
BspFNI CGCG 1 cut(s) 899
BspLI GGNNCC 3 cut(s) 559, 603, 753
BspMAI CTGCAG 1 cut(s) 478
BspOI GCTAGC 1 cut(s) 590
BspPI GGATC 2 cut(s) 253, 476
BspT104I TTCGAA 1 cut(s) 508
BspT107I GGYRCC 1 cut(s) 601
BsrDI GCAATG 2 cut(s) 575, 681
BsrFI RCCGGY 2 cut(s) 780, 901
BsrI ACTGG 3 cut(s) 61, 350, 803
BssAI RCCGGY 2 cut(s) 780, 901
BssHII GCGCGC 1 cut(s) 897
BssMI GATC 2 cut(s) 258, 481
Bst2UI CCWGG 3 cut(s) 389, 569, 810
Bst4CI ACNGT 4 cut(s) 193, 454, 849, 877
Bst6I CTCTTC 1 cut(s) 598
BstBI TTCGAA 1 cut(s) 508
BstC8I GCNNGC 4 cut(s) 83, 588, 782, 899
BstDEI CTNAG 3 cut(s) 495, 582, 761
BstENI CCTNNNNNAGG 1 cut(s) 240
BstFNI CGCG 1 cut(s) 899
BstHHI GCGC 2 cut(s) 899, 901
BstKTI GATC 2 cut(s) 261, 484
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 2 cut(s) 258, 481
BstMWI GCNNNNNNNGC 1 cut(s) 22
BstNI CCWGG 3 cut(s) 389, 569, 810
BstNSI RCATGY 1 cut(s) 85
BstSCI CCNGG 3 cut(s) 387, 567, 808
BstSFI CTRYAG 2 cut(s) 202, 474
BstUI CGCG 1 cut(s) 899
BstV1I GCAGC 1 cut(s) 804
BstV2I GAAGAC 2 cut(s) 81, 368
BstX2I RGATCY 2 cut(s) 258, 481
BstXI CCANNNNNNTGG 1 cut(s) 825
BstYI RGATCY 2 cut(s) 258, 481
BtgZI GCGATG 1 cut(s) 475
BtsIMutI CAGTG 6 cut(s) 68, 189, 343, 796, 845, 882
Cac8I GCNNGC 4 cut(s) 83, 588, 782, 899
CaiI CAGNNNCTG 1 cut(s) 798
CfoI GCGC 2 cut(s) 899, 901
Cfr10I RCCGGY 2 cut(s) 780, 901
Cfr13I GGNCC 3 cut(s) 280, 441, 752
Csp6I GTAC 4 cut(s) 199, 625, 742, 873
CviAII CATG 3 cut(s) 82, 825, 860
CviQI GTAC 4 cut(s) 199, 625, 742, 873
DdeI CTNAG 3 cut(s) 495, 582, 761
DpnI GATC 2 cut(s) 260, 483
DpnII GATC 2 cut(s) 258, 481
DraI TTTAAA 1 cut(s) 855
Eam1104I CTCTTC 1 cut(s) 598
EarI CTCTTC 1 cut(s) 598
Ecl136II GAGCTC 1 cut(s) 209
Eco24I GRGCYC 1 cut(s) 211
Eco47I GGWCC 3 cut(s) 280, 441, 752
Eco53kI GAGCTC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 209
EcoNI CCTNNNNNAGG 1 cut(s) 240
EcoO109I RGGNCCY 1 cut(s) 752
EcoRI GAATTC 1 cut(s) 295
EcoRII CCWGG 3 cut(s) 387, 567, 808
EcoT22I ATGCAT 2 cut(s) 83, 886
EcoT38I GRGCYC 1 cut(s) 211
FaeI CATG 3 cut(s) 85, 828, 863
FaqI GGGAC 2 cut(s) 32, 800
FatI CATG 3 cut(s) 81, 824, 859
FauI CCCGC 1 cut(s) 9
FauNDI CATATG 1 cut(s) 514
Fnu4HI GCNGC 1 cut(s) 793
FriOI GRGCYC 1 cut(s) 211
Fsp4HI GCNGC 1 cut(s) 793
FspBI CTAG 2 cut(s) 587, 870
GlaI GCGC 2 cut(s) 898, 900
GluI GCNGC 1 cut(s) 793
GsuI CTGGAG 1 cut(s) 831
HapII CCGG 2 cut(s) 781, 902
HhaI GCGC 2 cut(s) 899, 901
Hin1II CATG 3 cut(s) 85, 828, 863
Hin6I GCGC 2 cut(s) 897, 899
HinP1I GCGC 2 cut(s) 897, 899
HinfI GANTC 7 cut(s) 44, 68, 110, 493, 611, 619, 813
HpaII CCGG 2 cut(s) 781, 902
HphI GGTGA 3 cut(s) 53, 551, 557
Hpy188I TCNGA 3 cut(s) 263, 533, 717
Hpy188III TCNNGA 2 cut(s) 243, 300
HpyAV CCTTC 2 cut(s) 249, 386
HpyCH4III ACNGT 4 cut(s) 193, 454, 849, 877
HpyCH4V TGCA 9 cut(s) 81, 189, 226, 317, 476, 526, 792, 845, 884
HpyF10VI GCNNNNNNNGC 1 cut(s) 22
HpyF3I CTNAG 3 cut(s) 495, 582, 761
Hsp92II CATG 3 cut(s) 85, 828, 863
HspAI GCGC 2 cut(s) 897, 899
KroI GCCGGC 1 cut(s) 780
KroNI GCCGGC 1 cut(s) 782
Kzo9I GATC 2 cut(s) 258, 481
LmnI GCTCC 4 cut(s) 214, 245, 523, 557
Lsp1109I GCAGC 1 cut(s) 804
LweI GCATC 3 cut(s) 304, 418, 893
MaeI CTAG 2 cut(s) 587, 870
MaeIII GTNAC 3 cut(s) 380, 563, 697
MalI GATC 2 cut(s) 260, 483
MboI GATC 2 cut(s) 258, 481
MboII GAAGA 4 cut(s) 81, 373, 437, 585
MfeI CAATTG 1 cut(s) 704
MflI RGATCY 2 cut(s) 258, 481
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 5 cut(s) 138, 170, 295, 352, 704
MlyI GAGTC 4 cut(s) 487, 605, 628, 822
MnlI CCTC 7 cut(s) 246, 365, 527, 540, 601, 781, 858
Mph1103I ATGCAT 2 cut(s) 83, 886
MroNI GCCGGC 1 cut(s) 780
MseI TTAA 3 cut(s) 174, 692, 854
MslI CAYNNNNRTG 1 cut(s) 76
MspI CCGG 2 cut(s) 781, 902
MspR9I CCNGG 3 cut(s) 389, 569, 810
MunI CAATTG 1 cut(s) 704
Mva1269I GAATGC 1 cut(s) 886
MvaI CCWGG 3 cut(s) 389, 569, 810
MvnI CGCG 1 cut(s) 899
MwoI GCNNNNNNNGC 1 cut(s) 22
NaeI GCCGGC 1 cut(s) 782
NdeI CATATG 1 cut(s) 514
NdeII GATC 2 cut(s) 258, 481
NgoMIV GCCGGC 1 cut(s) 780
NheI GCTAGC 1 cut(s) 586
NlaIII CATG 3 cut(s) 85, 828, 863
NlaIV GGNNCC 3 cut(s) 559, 603, 753
NmuCI GTSAC 1 cut(s) 563
NsiI ATGCAT 2 cut(s) 83, 886
NspI RCATGY 1 cut(s) 85
NspV TTCGAA 1 cut(s) 508
PaeI GCATGC 1 cut(s) 85
PauI GCGCGC 1 cut(s) 897
PctI GAATGC 1 cut(s) 886
PdiI GCCGGC 1 cut(s) 782
PfeI GAWTC 3 cut(s) 44, 68, 110
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 1 cut(s) 794
PleI GAGTC 4 cut(s) 487, 605, 627, 821
PpsI GAGTC 4 cut(s) 487, 605, 627, 821
PpuMI RGGWCCY 1 cut(s) 752
Psp124BI GAGCTC 1 cut(s) 211
Psp5II RGGWCCY 1 cut(s) 752
Psp6I CCWGG 3 cut(s) 387, 567, 808
PspGI CCWGG 3 cut(s) 387, 567, 808
PspN4I GGNNCC 3 cut(s) 559, 603, 753
PspPI GGNCC 3 cut(s) 280, 441, 752
PspPPI RGGWCCY 1 cut(s) 752
PstI CTGCAG 1 cut(s) 478
PstNI CAGNNNCTG 1 cut(s) 798
PsuI RGATCY 2 cut(s) 258, 481
PteI GCGCGC 1 cut(s) 897
RsaI GTAC 4 cut(s) 200, 626, 743, 874
RsaNI GTAC 4 cut(s) 199, 625, 742, 873
RseI CAYNNNNRTG 1 cut(s) 76
SacI GAGCTC 1 cut(s) 211
SaqAI TTAA 3 cut(s) 174, 692, 854
SatI GCNGC 1 cut(s) 793
Sau3AI GATC 2 cut(s) 258, 481
Sau96I GGNCC 3 cut(s) 280, 441, 752
ScaI AGTACT 1 cut(s) 200
SchI GAGTC 4 cut(s) 487, 605, 628, 822
ScrFI CCNGG 3 cut(s) 389, 569, 810
SduI GDGCHC 1 cut(s) 211
SetI ASST 8 cut(s) 211, 250, 443, 501, 538, 570, 588, 655
SfaNI GCATC 3 cut(s) 304, 418, 893
SfcI CTRYAG 2 cut(s) 202, 474
SfuI TTCGAA 1 cut(s) 508
SinI GGWCC 3 cut(s) 280, 441, 752
SmiMI CAYNNNNRTG 1 cut(s) 76
SpeI ACTAGT 1 cut(s) 869
SphI GCATGC 1 cut(s) 85
Sse9I AATT 5 cut(s) 138, 170, 295, 352, 704
SsiI CCGC 1 cut(s) 16
SspMI CTAG 2 cut(s) 587, 870
SstI GAGCTC 1 cut(s) 211
StyD4I CCNGG 3 cut(s) 387, 567, 808
TaaI ACNGT 4 cut(s) 193, 454, 849, 877
TaqI TCGA 1 cut(s) 508
TasI AATT 5 cut(s) 138, 170, 295, 352, 704
TatI WGTACW 4 cut(s) 198, 624, 741, 872
TfiI GAWTC 3 cut(s) 44, 68, 110
Tru1I TTAA 3 cut(s) 174, 692, 854
Tru9I TTAA 3 cut(s) 174, 692, 854
TscAI CASTG 6 cut(s) 68, 196, 350, 803, 852, 882
TseFI GTSAC 1 cut(s) 563
TseI GCWGC 1 cut(s) 792
Tsp45I GTSAC 1 cut(s) 563
TspDTI ATGAA 5 cut(s) 135, 585, 692, 723, 846
TspRI CASTG 6 cut(s) 68, 196, 350, 803, 852, 882
VpaK11BI GGWCC 3 cut(s) 280, 441, 752
XagI CCTNNNNNAGG 1 cut(s) 240
XapI RAATTY 2 cut(s) 170, 295
XceI RCATGY 1 cut(s) 85
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XspI CTAG 2 cut(s) 587, 870
ZrmI AGTACT 1 cut(s) 200
Zsp2I ATGCAT 2 cut(s) 83, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.