Rh4CG414000

Domain in histone families 1 and 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0013660
Physical Location & Seq
Reverse (-)
65860823 .. 65862597
1775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG414000.1

Sequence Viewer

Length: 1305 bp
ATGGCAATCCCAAAACCTCAGCATCCCCTTCTCTTGGAACAGCAAAGGCAAGAACGCATAATGTGCAAACTCCGAGACGCCGTTTTGGCACGTGTGGCGGCGGCGGAGGCAGGAACCTCCAAACCCATGAACACCGCCGCCGACCACATTAACTCCGGCGTCGAACGCCGCCTCCAACAGCTCATCCCTAGTGTCTACACTCCCACTCATCCGCCTTATGCCTCGATGATACAAAGGGCAATAGAGGAGTTGAATGAGGAAGGAGGAGGTGTGAGCGAGGTTGCAATATCGAAGTTTATCAAAAGGGAATACGAGGATTTGCCGTGGGCGCATGAGGGTCTGTTGAGGCTTCATTTGAAGAAGCAATGTGAGATTGGGGTGCTTGTGTTAGATGGTGGGAGGTATAATTTTAATTTGGTTAAGGATGGTGATGGTGGGGCTTCTGTTGATGTTGAGACAGAGTCGAGGAGGAGAAGGCGGCCGGGGCGGGGTAGAAGAGGCCGGGGTAGATGGCGTGGCAGGGAAGAAGCGGAGCGGATTAATGAAGCATTTGAAGAAGAGGGGATTGAAGGAGGAGAAGGCCAAGATGAGGTGATGGGACAATCTGAGAGGCCAAACATGGATGAGGTGGAAAAGGCATTACTGGCATATATCAAATCGCATAACCGGGGAGAAGCAAACGAAGATGAGATGATAAAAGAACAGATTCGAGCAGGGGTGGGTGAAGATGAAGTTATAAAAAACAATAACCAAAGAGAAGAACAGCAAAGTGGAGAGGAGCCACAATCTCAGCATGATCAGCAACAACAGAAATCACAAGAACAAGGCCAAGTAAAGCGGCGTCCAGGGAGACCTAAAGCTAATAAAGTTGGGGATGTAGGTACTCTGGAAGTGGTACCCTGTGCTCCTGAGGAGCAGCCTTTGAGGCGTCGAGGGAGATCAAAAGCTAAAAATGATATGGATTTAGGTACCAATGCCGAAGTACCCTGTGCTGTTGAACCTCCTAATGAGGAGCAGCCCCAGAGGCGTCGAGGGAGGTCTAAAGCTGAACATGATATGGAGGCAAGTACTAGTAGTCTAGTACCTTGTGCTCTTGAACATCGACAGGAAGAGCAGCCACCGAAGCGACGAGGCCCAGGGAGACCTCCTAAACCAAAGCCAGATTCAGAAGCTACCTTGACAGTTTTGTCAAGCTCAGATATTCCGCATCACTCTAAGCAACAACAGCCCCTGAGCCAAGCAACTAAGATGAGGTTTTCTAAACCAAAGCGAGGCAGAGGGAGGCCTCGAACCCTCAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Linker_histone

Protein Analysis

434

Amino Acids

48.87

Weight (kDa)

8.68

Isoelectric Point (pI)

70.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 70 - 130 1.2e-09 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 737
AasI GACNNNNNNGTC 1 cut(s) 1186
Acc65I GGTACC 2 cut(s) 895, 968
AccB1I GGYRCC 2 cut(s) 895, 968
AccBSI CCGCTC 1 cut(s) 535
AccI GTMKAC 1 cut(s) 195
AcoI YGGCCR 1 cut(s) 479
AcvI CACGTG 1 cut(s) 92
AcyI GRCGYC 5 cut(s) 78, 159, 841, 928, 1027
AfaI GTAC 6 cut(s) 883, 897, 970, 984, 1069, 1083
AfiI CCNNNNNNNGG 4 cut(s) 34, 488, 589, 1271
AflIII ACRYGT 1 cut(s) 91
AgsI TTSAA 6 cut(s) 253, 358, 554, 569, 998, 1097
AhlI ACTAGT 1 cut(s) 1070
AjnI CCWGG 2 cut(s) 844, 1135
AjuI GAANNNNNNNTTGG 2 cut(s) 1147, 1179
AloI GAACNNNNNNTCC 2 cut(s) 156, 188
AluBI AGCT 6 cut(s) 181, 860, 947, 1046, 1172, 1194
AluI AGCT 6 cut(s) 181, 860, 947, 1046, 1172, 1194
Alw21I GWGCWC 2 cut(s) 907, 1093
Alw26I GTCTC 4 cut(s) 69, 449, 844, 1135
AlwNI CAGNNNCTG 2 cut(s) 1231, 1302
AoxI GGCC 7 cut(s) 479, 499, 580, 611, 826, 1132, 1283
ApeKI GCWGC 3 cut(s) 916, 1015, 1114
AseI ATTAAT 1 cut(s) 540
Asp700I GAANNNNTTC 1 cut(s) 705
Asp718I GGTACC 2 cut(s) 895, 968
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 1133
AsuC2I CCSGG 3 cut(s) 483, 503, 668
AsuHPI GGTGA 3 cut(s) 440, 604, 734
AxyI CCTNAGG 1 cut(s) 909
BanI GGYRCC 2 cut(s) 895, 968
BbrPI CACGTG 1 cut(s) 92
Bbv12I GWGCWC 2 cut(s) 907, 1093
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 3 cut(s) 928, 1027, 1126
BccI CCATC 5 cut(s) 386, 419, 425, 504, 589
BceAI ACGGC 2 cut(s) 65, 307
BciT130I CCWGG 2 cut(s) 846, 1137
BclI TGATCA 1 cut(s) 796
BcnI CCSGG 3 cut(s) 483, 503, 668
BcoDI GTCTC 4 cut(s) 69, 449, 844, 1135
BcuI ACTAGT 1 cut(s) 1070
BfaI CTAG 3 cut(s) 189, 1071, 1079
BglI GCCNNNNNGGC 3 cut(s) 86, 925, 1024
BisI GCNGC 9 cut(s) 99, 102, 138, 169, 479, 839, 917, 1016, 1115
BlsI GCNGC 9 cut(s) 100, 103, 139, 170, 480, 840, 918, 1017, 1116
BmcAI AGTACT 1 cut(s) 1069
Bme1390I CCNGG 5 cut(s) 483, 503, 668, 846, 1137
BmgT120I GGNCC 1 cut(s) 1133
BmiI GGNNCC 4 cut(s) 115, 780, 897, 970
BmrFI CCNGG 5 cut(s) 483, 503, 668, 846, 1137
BmsI GCATC 2 cut(s) 31, 1216
Bpu10I CCTNAGC 2 cut(s) 18, 1232
BpuMI CCSGG 3 cut(s) 483, 503, 668
BsaAI YACGTR 1 cut(s) 92
BsaHI GRCGYC 5 cut(s) 78, 159, 841, 928, 1027
BsaI GGTCTC 2 cut(s) 844, 1135
BsaJI CCNNGG 7 cut(s) 323, 482, 502, 667, 845, 1135, 1136
BsaXI ACNNNNNCTCC 6 cut(s) 137, 156, 167, 186, 255, 285
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 488, 589, 1271
Bse1I ACTGG 1 cut(s) 648
Bse21I CCTNAGG 1 cut(s) 909
Bse3DI GCAATG 1 cut(s) 371
BseBI CCWGG 2 cut(s) 846, 1137
BseDI CCNNGG 7 cut(s) 323, 482, 502, 667, 845, 1135, 1136
BseGI GGATG 6 cut(s) 22, 183, 208, 430, 628, 880
BseLI CCNNNNNNNGG 4 cut(s) 34, 488, 589, 1271
BseMI GCAATG 1 cut(s) 371
BseMII CTCAG 6 cut(s) 32, 597, 803, 900, 1209, 1223
BseNI ACTGG 1 cut(s) 648
BseRI GAGGAG 8 cut(s) 260, 279, 481, 484, 588, 791, 926, 1025
BseX3I CGGCCG 1 cut(s) 479
BseXI GCAGC 3 cut(s) 928, 1027, 1126
Bsh1285I CGRYCG 1 cut(s) 482
BshFI GGCC 7 cut(s) 481, 501, 582, 613, 828, 1134, 1285
BshNI GGYRCC 2 cut(s) 895, 968
BsiEI CGRYCG 1 cut(s) 482
BsiHKAI GWGCWC 2 cut(s) 907, 1093
BsiSI CCGG 4 cut(s) 156, 482, 502, 667
BslFI GGGAC 1 cut(s) 612
BslI CCNNNNNNNGG 4 cut(s) 34, 488, 589, 1271
BsmAI GTCTC 4 cut(s) 69, 449, 844, 1135
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 612
BsnI GGCC 7 cut(s) 481, 501, 582, 613, 828, 1134, 1285
Bso31I GGTCTC 2 cut(s) 844, 1135
Bsp1286I GDGCHC 2 cut(s) 907, 1093
Bsp143I GATC 2 cut(s) 796, 938
BspANI GGCC 7 cut(s) 481, 501, 582, 613, 828, 1134, 1285
BspCNI CTCAG 6 cut(s) 31, 598, 802, 901, 1208, 1224
BspLI GGNNCC 4 cut(s) 115, 780, 897, 970
BspQI GCTCTTC 1 cut(s) 1104
BspT107I GGYRCC 2 cut(s) 895, 968
BspTNI GGTCTC 2 cut(s) 844, 1135
BsrBI CCGCTC 1 cut(s) 535
BsrDI GCAATG 1 cut(s) 371
BsrI ACTGG 1 cut(s) 648
BssECI CCNNGG 7 cut(s) 323, 482, 502, 667, 845, 1135, 1136
BssMI GATC 2 cut(s) 796, 938
BssNI GRCGYC 5 cut(s) 78, 159, 841, 928, 1027
Bst2UI CCWGG 2 cut(s) 846, 1137
Bst4CI ACNGT 1 cut(s) 1183
Bst6I CTCTTC 3 cut(s) 490, 552, 1104
BstACI GRCGYC 5 cut(s) 78, 159, 841, 928, 1027
BstAPI GCANNNNNTGC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 92
BstDEI CTNAG 9 cut(s) 18, 606, 789, 909, 1195, 1215, 1232, 1245, 1295
BstDSI CCRYGG 1 cut(s) 323
BstF5I GGATG 6 cut(s) 22, 183, 208, 430, 628, 880
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 2 cut(s) 799, 941
BstMAI GTCTC 4 cut(s) 69, 449, 844, 1135
BstMBI GATC 2 cut(s) 796, 938
BstMCI CGRYCG 1 cut(s) 482
BstNI CCWGG 2 cut(s) 846, 1137
BstSCI CCNGG 5 cut(s) 481, 501, 666, 844, 1135
BstV1I GCAGC 3 cut(s) 928, 1027, 1126
BstZI CGGCCG 1 cut(s) 479
Bsu36I CCTNAGG 1 cut(s) 909
BsuRI GGCC 7 cut(s) 481, 501, 582, 613, 828, 1134, 1285
BtgI CCRYGG 1 cut(s) 323
BtsCI GGATG 6 cut(s) 22, 183, 208, 430, 628, 880
CaiI CAGNNNCTG 2 cut(s) 1231, 1302
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 1133
CseI GACGC 5 cut(s) 86, 148, 830, 917, 1016
Csp6I GTAC 6 cut(s) 882, 896, 969, 983, 1068, 1082
CviAII CATG 5 cut(s) 127, 332, 619, 794, 1052
CviQI GTAC 6 cut(s) 882, 896, 969, 983, 1068, 1082
DdeI CTNAG 9 cut(s) 18, 606, 789, 909, 1195, 1215, 1232, 1245, 1295
DpnI GATC 2 cut(s) 798, 940
DpnII GATC 2 cut(s) 796, 938
DrdI GACNNNNNNGTC 1 cut(s) 1186
DseDI GACNNNNNNGTC 1 cut(s) 1186
EaeI YGGCCR 1 cut(s) 479
EagI CGGCCG 1 cut(s) 479
Eam1104I CTCTTC 3 cut(s) 490, 552, 1104
EarI CTCTTC 3 cut(s) 490, 552, 1104
EciI GGCGGA 2 cut(s) 119, 201
EclXI CGGCCG 1 cut(s) 479
Eco147I AGGCCT 1 cut(s) 1285
Eco31I GGTCTC 2 cut(s) 844, 1135
Eco52I CGGCCG 1 cut(s) 479
Eco72I CACGTG 1 cut(s) 92
Eco81I CCTNAGG 1 cut(s) 909
EcoRII CCWGG 2 cut(s) 844, 1135
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 5 cut(s) 130, 335, 622, 797, 1055
FaqI GGGAC 1 cut(s) 612
FatI CATG 5 cut(s) 126, 331, 618, 793, 1051
FauI CCCGC 1 cut(s) 480
FbaI TGATCA 1 cut(s) 796
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 9 cut(s) 99, 102, 138, 169, 479, 839, 917, 1016, 1115
FokI GGATG 6 cut(s) 9, 170, 195, 437, 635, 887
Fsp4HI GCNGC 9 cut(s) 99, 102, 138, 169, 479, 839, 917, 1016, 1115
FspBI CTAG 3 cut(s) 189, 1071, 1079
GlaI GCGC 1 cut(s) 330
GluI GCNGC 9 cut(s) 99, 102, 138, 169, 479, 839, 917, 1016, 1115
HaeIII GGCC 7 cut(s) 481, 501, 582, 613, 828, 1134, 1285
HapII CCGG 4 cut(s) 156, 482, 502, 667
HgaI GACGC 5 cut(s) 86, 148, 830, 917, 1016
HhaI GCGC 1 cut(s) 331
Hin1I GRCGYC 5 cut(s) 78, 159, 841, 928, 1027
Hin1II CATG 5 cut(s) 130, 335, 622, 797, 1055
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HinfI GANTC 3 cut(s) 461, 706, 1163
HpaII CCGG 4 cut(s) 156, 482, 502, 667
HphI GGTGA 3 cut(s) 440, 604, 734
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 5 cut(s) 74, 607, 1168, 1198, 1298
Hpy188III TCNNGA 3 cut(s) 887, 908, 1094
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 4 cut(s) 164, 933, 1032, 1131
HpyAV CCTTC 5 cut(s) 38, 254, 468, 563, 572
HpyCH4III ACNGT 1 cut(s) 1183
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 2 cut(s) 66, 284
HpyF3I CTNAG 9 cut(s) 18, 606, 789, 909, 1195, 1215, 1232, 1245, 1295
HpySE526I ACGT 1 cut(s) 91
Hsp92I GRCGYC 5 cut(s) 78, 159, 841, 928, 1027
Hsp92II CATG 5 cut(s) 130, 335, 622, 797, 1055
HspAI GCGC 1 cut(s) 329
KpnI GGTACC 2 cut(s) 899, 972
Ksp22I TGATCA 1 cut(s) 796
Kzo9I GATC 2 cut(s) 796, 938
LguI GCTCTTC 1 cut(s) 1104
LmnI GCTCC 5 cut(s) 532, 778, 910, 913, 1012
Lsp1109I GCAGC 3 cut(s) 928, 1027, 1126
LweI GCATC 2 cut(s) 31, 1216
MaeI CTAG 3 cut(s) 189, 1071, 1079
MaeII ACGT 1 cut(s) 91
MalI GATC 2 cut(s) 798, 940
MbiI CCGCTC 1 cut(s) 535
MboI GATC 2 cut(s) 796, 938
MboII GAAGA 9 cut(s) 370, 507, 536, 566, 569, 695, 737, 770, 1121
MhlI GDGCHC 2 cut(s) 907, 1093
MluCI AATT 2 cut(s) 406, 412
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 1 cut(s) 199
MroXI GAANNNNTTC 1 cut(s) 705
MseI TTAA 4 cut(s) 150, 411, 420, 540
MspI CCGG 4 cut(s) 156, 482, 502, 667
MspR9I CCNGG 5 cut(s) 483, 503, 668, 846, 1137
MvaI CCWGG 2 cut(s) 846, 1137
NciI CCSGG 3 cut(s) 483, 503, 668
NdeII GATC 2 cut(s) 796, 938
NlaIII CATG 5 cut(s) 130, 335, 622, 797, 1055
NlaIV GGNNCC 4 cut(s) 115, 780, 897, 970
PasI CCCWGGG 1 cut(s) 1136
PceI AGGCCT 1 cut(s) 1285
PciSI GCTCTTC 1 cut(s) 1104
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 2 cut(s) 706, 1163
PflFI GACNNNGTC 1 cut(s) 460
PkrI GCNGC 9 cut(s) 100, 103, 139, 170, 480, 840, 918, 1017, 1116
PleI GAGTC 1 cut(s) 469
PmaCI CACGTG 1 cut(s) 92
PmlI CACGTG 1 cut(s) 92
PpsI GAGTC 1 cut(s) 469
Ppu21I YACGTR 1 cut(s) 92
PshBI ATTAAT 1 cut(s) 540
PsiI TTATAA 1 cut(s) 737
Psp6I CCWGG 2 cut(s) 844, 1135
PspCI CACGTG 1 cut(s) 92
PspGI CCWGG 2 cut(s) 844, 1135
PspN4I GGNNCC 4 cut(s) 115, 780, 897, 970
PspPI GGNCC 1 cut(s) 1133
PstNI CAGNNNCTG 2 cut(s) 1231, 1302
PsyI GACNNNGTC 1 cut(s) 460
RsaI GTAC 6 cut(s) 883, 897, 970, 984, 1069, 1083
RsaNI GTAC 6 cut(s) 882, 896, 969, 983, 1068, 1082
SapI GCTCTTC 1 cut(s) 1104
SaqAI TTAA 4 cut(s) 150, 411, 420, 540
SatI GCNGC 9 cut(s) 99, 102, 138, 169, 479, 839, 917, 1016, 1115
Sau3AI GATC 2 cut(s) 796, 938
Sau96I GGNCC 1 cut(s) 1133
ScaI AGTACT 1 cut(s) 1069
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 5 cut(s) 483, 503, 668, 846, 1137
SduI GDGCHC 2 cut(s) 907, 1093
SfaNI GCATC 2 cut(s) 31, 1216
SpeI ACTAGT 1 cut(s) 1070
Sse9I AATT 2 cut(s) 406, 412
SseBI AGGCCT 1 cut(s) 1285
SspMI CTAG 3 cut(s) 189, 1071, 1079
StuI AGGCCT 1 cut(s) 1285
StyD4I CCNGG 5 cut(s) 481, 501, 666, 844, 1135
TaaI ACNGT 1 cut(s) 1183
TaiI ACGT 1 cut(s) 94
TaqI TCGA 9 cut(s) 162, 224, 290, 464, 709, 931, 1030, 1102, 1288
TasI AATT 2 cut(s) 406, 412
TatI WGTACW 1 cut(s) 1067
TauI GCSGC 6 cut(s) 101, 104, 140, 171, 481, 841
TfiI GAWTC 2 cut(s) 706, 1163
Tru1I TTAA 4 cut(s) 150, 411, 420, 540
Tru9I TTAA 4 cut(s) 150, 411, 420, 540
TseI GCWGC 3 cut(s) 916, 1015, 1114
TspDTI ATGAA 4 cut(s) 143, 341, 558, 744
Tth111I GACNNNGTC 1 cut(s) 460
VspI ATTAAT 1 cut(s) 540
XmiI GTMKAC 1 cut(s) 195
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 3 cut(s) 189, 1071, 1079
ZrmI AGTACT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.