Rw4G033230

Domain in histone families 1 and 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013660
Physical Location & Seq
Reverse (-)
60310783 .. 60312597
1815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033230.1

Sequence Viewer

Length: 1275 bp
ATGGCAATGCCAAAACCTCAGCATCCCCTTCAGCTTTGTCTGCAAAACTTGGAACGGCAAAAGCAAGAGCGCATAATGTGCAAACTCCGAGACGCCGTTTTGGCACGTGTGGCGGCGGCGGAGGCAGGAACCTCCAAACCCATGAACACCGCCGACCACATTAACTCCGGCGTCGAACGCCGCCTCCAACAGCTCATCCCTAGTGTCTGCACTCCCACTCACCCGCCTTATGCCTCGATGATACAAAGGGCAATAGAGGAGTTGAAGGAGGAAGGAGGAGGTGTGAGCGAGGTTGCAATATCGAAGTTTATCAAAAGGGAATACGAGGATTTGCCGTGGGCGCATGAGGGTCTGTTGAGGCTTCATTTGAAGAAGCAATGTGAGATTGGGGTGCTTGTGTTAAATGGTGGGAGGTATAATTTTAATTTGGTTGAGGATGGTGATGGTGGGGCTTCTGTTGATGTTGAGACAGAGTCGAGGAGGAGAAGGCGGCCGGGGCGGGGTAGAAGAGGCCGGGGTAGATGGTGTGGCAGGGAAGAAGCGGAGCGGATTAATGAAGCATTTGAAGAAGAGGGGATTGAAGGAGGAGAAGACCAAGATGAGGTGATGGGACAATCTGAGGTACTACTTAGGCCAAACATGGATGAGGTGGAAAAGGCATTACTGGCATATATCAAATCGCATAACTGGGGAGAAGCAAACAAAGATGGGATGATAAAAGAACAGATTCGAGAAGGGGTGGGTGAAGATGAAGTTATAAAAAACAATAACCAAAGAGAAGAACAGCAAAAACAAGGCCAAGTAAAGCGGCGTCCAGGGAGACCTAAAGCTAATAAAGTTGGGGATGTAGGTACTCTGGAAGTGGTACCCTGTGCTCCTGAGGAGCAGCCTTTGAGGCGTCAAGGGAGATCAAAAGCTAAAAATGATATGGATTTAGGTACCAATGCTGAAGTACCCTGTGCTGTTGAACCTCCTAATGAGGAGCAGCCCCAGAGGCGTCGAGGGAGGTCTAAAGCTAAACATGATATGGAGGCAAGTACTAGTAGTCTAGTACCTTGTGCTCTTGAACATCGACAGGAAGAGCAGCCACCGAAGCGACGAGGCCCAGGGAGACCTCCTAAACCAAAGCCAGATTCAGAAGTTACCTTGACAGTTTTGTCAAGCTCAGATATTCCGCATCACTCTAAGCAACAACAGCCCCTGAGCCAAGCAACTAAGATGAGGTTTTGTAAACCAAAGCGAGGCAGAGGGAGGCCTCGAACCCTCAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Linker_histone

Protein Analysis

424

Amino Acids

47.77

Weight (kDa)

9.04

Isoelectric Point (pI)

69.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 74 - 134 5.8e-10 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 758
AasI GACNNNNNNGTC 1 cut(s) 1156
Acc65I GGTACC 2 cut(s) 865, 938
AccB1I GGYRCC 2 cut(s) 865, 938
AccBSI CCGCTC 1 cut(s) 547
AcoI YGGCCR 1 cut(s) 491
AcuI CTGAAG 2 cut(s) 14, 969
AcvI CACGTG 1 cut(s) 107
AcyI GRCGYC 5 cut(s) 93, 171, 811, 898, 997
AfaI GTAC 7 cut(s) 624, 853, 867, 940, 954, 1039, 1053
AfiI CCNNNNNNNGG 3 cut(s) 500, 601, 1241
AflIII ACRYGT 1 cut(s) 106
AgsI TTSAA 6 cut(s) 265, 370, 566, 581, 968, 1067
AhlI ACTAGT 1 cut(s) 1040
AjnI CCWGG 2 cut(s) 814, 1105
AjuI GAANNNNNNNTTGG 2 cut(s) 1117, 1149
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
AluBI AGCT 6 cut(s) 34, 193, 830, 917, 1016, 1164
AluI AGCT 6 cut(s) 34, 193, 830, 917, 1016, 1164
Alw21I GWGCWC 2 cut(s) 877, 1063
Alw26I GTCTC 4 cut(s) 84, 461, 814, 1105
AlwNI CAGNNNCTG 2 cut(s) 1201, 1272
AoxI GGCC 6 cut(s) 491, 511, 632, 796, 1102, 1253
ApeKI GCWGC 3 cut(s) 886, 985, 1084
AseI ATTAAT 1 cut(s) 552
Asp700I GAANNNNTTC 1 cut(s) 726
Asp718I GGTACC 2 cut(s) 865, 938
AspLEI GCGC 2 cut(s) 72, 343
AspS9I GGNCC 1 cut(s) 1103
AsuC2I CCSGG 2 cut(s) 495, 515
AsuHPI GGTGA 4 cut(s) 212, 452, 616, 755
AxyI CCTNAGG 1 cut(s) 879
BanI GGYRCC 2 cut(s) 865, 938
BbrPI CACGTG 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 2 cut(s) 877, 1063
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 3 cut(s) 898, 997, 1096
BccI CCATC 5 cut(s) 431, 437, 516, 601, 701
BceAI ACGGC 3 cut(s) 71, 80, 319
BciT130I CCWGG 2 cut(s) 816, 1107
BcnI CCSGG 2 cut(s) 495, 515
BcoDI GTCTC 4 cut(s) 84, 461, 814, 1105
BcuI ACTAGT 1 cut(s) 1040
BfaI CTAG 3 cut(s) 201, 1041, 1049
BglI GCCNNNNNGGC 3 cut(s) 101, 895, 994
BisI GCNGC 8 cut(s) 114, 117, 181, 491, 809, 887, 986, 1085
BlsI GCNGC 8 cut(s) 115, 118, 182, 492, 810, 888, 987, 1086
BmcAI AGTACT 1 cut(s) 1039
Bme1390I CCNGG 4 cut(s) 495, 515, 816, 1107
BmgT120I GGNCC 1 cut(s) 1103
BmiI GGNNCC 3 cut(s) 130, 867, 940
BmrFI CCNGG 4 cut(s) 495, 515, 816, 1107
BmrI ACTGGG 1 cut(s) 697
BmsI GCATC 2 cut(s) 31, 1186
BmuI ACTGGG 1 cut(s) 697
BpiI GAAGAC 1 cut(s) 597
Bpu10I CCTNAGC 2 cut(s) 18, 1202
BpuMI CCSGG 2 cut(s) 495, 515
BsaAI YACGTR 1 cut(s) 107
BsaHI GRCGYC 5 cut(s) 93, 171, 811, 898, 997
BsaI GGTCTC 2 cut(s) 814, 1105
BsaJI CCNNGG 6 cut(s) 335, 494, 514, 815, 1105, 1106
BsaXI ACNNNNNCTCC 6 cut(s) 149, 168, 179, 198, 267, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 500, 601, 1241
Bse1I ACTGG 2 cut(s) 669, 692
Bse21I CCTNAGG 1 cut(s) 879
Bse3DI GCAATG 2 cut(s) 12, 383
BseBI CCWGG 2 cut(s) 816, 1107
BseDI CCNNGG 6 cut(s) 335, 494, 514, 815, 1105, 1106
BseGI GGATG 6 cut(s) 22, 195, 442, 649, 717, 850
BseLI CCNNNNNNNGG 3 cut(s) 500, 601, 1241
BseMI GCAATG 2 cut(s) 12, 383
BseMII CTCAG 5 cut(s) 32, 609, 870, 1179, 1193
BseNI ACTGG 2 cut(s) 669, 692
BseRI GAGGAG 7 cut(s) 272, 291, 493, 496, 600, 896, 995
BseX3I CGGCCG 1 cut(s) 491
BseXI GCAGC 3 cut(s) 898, 997, 1096
BsgI GTGCAG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 494
BshFI GGCC 6 cut(s) 493, 513, 634, 798, 1104, 1255
BshNI GGYRCC 2 cut(s) 865, 938
BsiEI CGRYCG 1 cut(s) 494
BsiHKAI GWGCWC 2 cut(s) 877, 1063
BsiSI CCGG 3 cut(s) 168, 494, 514
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 3 cut(s) 500, 601, 1241
BsmAI GTCTC 4 cut(s) 84, 461, 814, 1105
BsmBI CGTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 624
BsnI GGCC 6 cut(s) 493, 513, 634, 798, 1104, 1255
Bso31I GGTCTC 2 cut(s) 814, 1105
Bsp1286I GDGCHC 2 cut(s) 877, 1063
Bsp143I GATC 1 cut(s) 908
BspANI GGCC 6 cut(s) 493, 513, 634, 798, 1104, 1255
BspCNI CTCAG 5 cut(s) 31, 610, 871, 1178, 1194
BspLI GGNNCC 3 cut(s) 130, 867, 940
BspQI GCTCTTC 1 cut(s) 1074
BspT107I GGYRCC 2 cut(s) 865, 938
BspTNI GGTCTC 2 cut(s) 814, 1105
BsrBI CCGCTC 1 cut(s) 547
BsrDI GCAATG 2 cut(s) 12, 383
BsrI ACTGG 2 cut(s) 669, 692
BssECI CCNNGG 6 cut(s) 335, 494, 514, 815, 1105, 1106
BssMI GATC 1 cut(s) 908
BssNI GRCGYC 5 cut(s) 93, 171, 811, 898, 997
Bst2UI CCWGG 2 cut(s) 816, 1107
Bst4CI ACNGT 1 cut(s) 1153
Bst6I CTCTTC 3 cut(s) 502, 564, 1074
BstACI GRCGYC 5 cut(s) 93, 171, 811, 898, 997
BstAPI GCANNNNNTGC 1 cut(s) 78
BstBAI YACGTR 1 cut(s) 107
BstDEI CTNAG 9 cut(s) 18, 618, 629, 879, 1165, 1185, 1202, 1215, 1265
BstDSI CCRYGG 1 cut(s) 335
BstF5I GGATG 6 cut(s) 22, 195, 442, 649, 717, 850
BstHHI GCGC 2 cut(s) 72, 343
BstKTI GATC 1 cut(s) 911
BstMAI GTCTC 4 cut(s) 84, 461, 814, 1105
BstMBI GATC 1 cut(s) 908
BstMCI CGRYCG 1 cut(s) 494
BstNI CCWGG 2 cut(s) 816, 1107
BstSCI CCNGG 4 cut(s) 493, 513, 814, 1105
BstV1I GCAGC 3 cut(s) 898, 997, 1096
BstV2I GAAGAC 1 cut(s) 597
BstZI CGGCCG 1 cut(s) 491
Bsu36I CCTNAGG 1 cut(s) 879
BsuRI GGCC 6 cut(s) 493, 513, 634, 798, 1104, 1255
BtgI CCRYGG 1 cut(s) 335
BtsCI GGATG 6 cut(s) 22, 195, 442, 649, 717, 850
CaiI CAGNNNCTG 2 cut(s) 1201, 1272
CfoI GCGC 2 cut(s) 72, 343
Cfr13I GGNCC 1 cut(s) 1103
CseI GACGC 5 cut(s) 101, 160, 800, 887, 986
Csp6I GTAC 7 cut(s) 623, 852, 866, 939, 953, 1038, 1052
CviAII CATG 4 cut(s) 142, 344, 640, 1022
CviQI GTAC 7 cut(s) 623, 852, 866, 939, 953, 1038, 1052
DdeI CTNAG 9 cut(s) 18, 618, 629, 879, 1165, 1185, 1202, 1215, 1265
DpnI GATC 1 cut(s) 910
DpnII GATC 1 cut(s) 908
DrdI GACNNNNNNGTC 1 cut(s) 1156
DseDI GACNNNNNNGTC 1 cut(s) 1156
EaeI YGGCCR 1 cut(s) 491
EagI CGGCCG 1 cut(s) 491
Eam1104I CTCTTC 3 cut(s) 502, 564, 1074
EarI CTCTTC 3 cut(s) 502, 564, 1074
EciI GGCGGA 1 cut(s) 134
EclXI CGGCCG 1 cut(s) 491
Eco147I AGGCCT 1 cut(s) 1255
Eco31I GGTCTC 2 cut(s) 814, 1105
Eco52I CGGCCG 1 cut(s) 491
Eco57I CTGAAG 2 cut(s) 14, 969
Eco72I CACGTG 1 cut(s) 107
Eco81I CCTNAGG 1 cut(s) 879
EcoRII CCWGG 2 cut(s) 814, 1105
Esp3I CGTCTC 1 cut(s) 84
FaeI CATG 4 cut(s) 145, 347, 643, 1025
FaqI GGGAC 1 cut(s) 624
FatI CATG 4 cut(s) 141, 343, 639, 1021
FauI CCCGC 2 cut(s) 231, 492
Fnu4HI GCNGC 8 cut(s) 114, 117, 181, 491, 809, 887, 986, 1085
FokI GGATG 6 cut(s) 9, 182, 449, 656, 724, 857
Fsp4HI GCNGC 8 cut(s) 114, 117, 181, 491, 809, 887, 986, 1085
FspBI CTAG 3 cut(s) 201, 1041, 1049
GlaI GCGC 2 cut(s) 71, 342
GluI GCNGC 8 cut(s) 114, 117, 181, 491, 809, 887, 986, 1085
HaeIII GGCC 6 cut(s) 493, 513, 634, 798, 1104, 1255
HapII CCGG 3 cut(s) 168, 494, 514
HgaI GACGC 5 cut(s) 101, 160, 800, 887, 986
HhaI GCGC 2 cut(s) 72, 343
Hin1I GRCGYC 5 cut(s) 93, 171, 811, 898, 997
Hin1II CATG 4 cut(s) 145, 347, 643, 1025
Hin6I GCGC 2 cut(s) 70, 341
HinP1I GCGC 2 cut(s) 70, 341
HinfI GANTC 3 cut(s) 473, 727, 1133
HpaII CCGG 3 cut(s) 168, 494, 514
HphI GGTGA 4 cut(s) 212, 452, 616, 755
Hpy166II GTNNAC 1 cut(s) 1232
Hpy188I TCNGA 5 cut(s) 89, 619, 1138, 1168, 1268
Hpy188III TCNNGA 4 cut(s) 731, 857, 878, 1064
Hpy8I GTNNAC 1 cut(s) 1232
Hpy99I CGWCG 3 cut(s) 176, 1002, 1101
HpyAV CCTTC 6 cut(s) 38, 259, 266, 480, 575, 728
HpyCH4III ACNGT 1 cut(s) 1153
HpyCH4IV ACGT 1 cut(s) 106
HpyCH4V TGCA 4 cut(s) 43, 81, 210, 296
HpyF3I CTNAG 9 cut(s) 18, 618, 629, 879, 1165, 1185, 1202, 1215, 1265
HpySE526I ACGT 1 cut(s) 106
Hsp92I GRCGYC 5 cut(s) 93, 171, 811, 898, 997
Hsp92II CATG 4 cut(s) 145, 347, 643, 1025
HspAI GCGC 2 cut(s) 70, 341
KpnI GGTACC 2 cut(s) 869, 942
Kzo9I GATC 1 cut(s) 908
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 4 cut(s) 544, 880, 883, 982
Lsp1109I GCAGC 3 cut(s) 898, 997, 1096
LweI GCATC 2 cut(s) 31, 1186
MaeI CTAG 3 cut(s) 201, 1041, 1049
MaeII ACGT 1 cut(s) 106
MaeIII GTNAC 1 cut(s) 1141
MalI GATC 1 cut(s) 910
MbiI CCGCTC 1 cut(s) 547
MboI GATC 1 cut(s) 908
MboII GAAGA 9 cut(s) 382, 519, 548, 578, 581, 602, 758, 791, 1091
MhlI GDGCHC 2 cut(s) 877, 1063
MluCI AATT 2 cut(s) 418, 424
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 211
MroXI GAANNNNTTC 1 cut(s) 726
MseI TTAA 4 cut(s) 162, 401, 423, 552
MspI CCGG 3 cut(s) 168, 494, 514
MspR9I CCNGG 4 cut(s) 495, 515, 816, 1107
MvaI CCWGG 2 cut(s) 816, 1107
NciI CCSGG 2 cut(s) 495, 515
NdeII GATC 1 cut(s) 908
NlaIII CATG 4 cut(s) 145, 347, 643, 1025
NlaIV GGNNCC 3 cut(s) 130, 867, 940
PasI CCCWGGG 1 cut(s) 1106
PceI AGGCCT 1 cut(s) 1255
PciSI GCTCTTC 1 cut(s) 1074
PdmI GAANNNNTTC 1 cut(s) 726
PfeI GAWTC 2 cut(s) 727, 1133
PflFI GACNNNGTC 1 cut(s) 472
PkrI GCNGC 8 cut(s) 115, 118, 182, 492, 810, 888, 987, 1086
PleI GAGTC 1 cut(s) 481
PmaCI CACGTG 1 cut(s) 107
PmlI CACGTG 1 cut(s) 107
PpsI GAGTC 1 cut(s) 481
Ppu21I YACGTR 1 cut(s) 107
PshBI ATTAAT 1 cut(s) 552
PsiI TTATAA 1 cut(s) 758
Psp6I CCWGG 2 cut(s) 814, 1105
PspCI CACGTG 1 cut(s) 107
PspGI CCWGG 2 cut(s) 814, 1105
PspN4I GGNNCC 3 cut(s) 130, 867, 940
PspPI GGNCC 1 cut(s) 1103
PstNI CAGNNNCTG 2 cut(s) 1201, 1272
PsyI GACNNNGTC 1 cut(s) 472
RsaI GTAC 7 cut(s) 624, 853, 867, 940, 954, 1039, 1053
RsaNI GTAC 7 cut(s) 623, 852, 866, 939, 953, 1038, 1052
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 4 cut(s) 162, 401, 423, 552
SatI GCNGC 8 cut(s) 114, 117, 181, 491, 809, 887, 986, 1085
Sau3AI GATC 1 cut(s) 908
Sau96I GGNCC 1 cut(s) 1103
ScaI AGTACT 1 cut(s) 1039
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 4 cut(s) 495, 515, 816, 1107
SduI GDGCHC 2 cut(s) 877, 1063
SfaNI GCATC 2 cut(s) 31, 1186
SpeI ACTAGT 1 cut(s) 1040
Sse9I AATT 2 cut(s) 418, 424
SseBI AGGCCT 1 cut(s) 1255
SspMI CTAG 3 cut(s) 201, 1041, 1049
StuI AGGCCT 1 cut(s) 1255
StyD4I CCNGG 4 cut(s) 493, 513, 814, 1105
TaaI ACNGT 1 cut(s) 1153
TaiI ACGT 1 cut(s) 109
TaqI TCGA 8 cut(s) 174, 236, 302, 476, 730, 1000, 1072, 1258
TasI AATT 2 cut(s) 418, 424
TatI WGTACW 1 cut(s) 1037
TauI GCSGC 5 cut(s) 116, 119, 183, 493, 811
TfiI GAWTC 2 cut(s) 727, 1133
Tru1I TTAA 4 cut(s) 162, 401, 423, 552
Tru9I TTAA 4 cut(s) 162, 401, 423, 552
TseI GCWGC 3 cut(s) 886, 985, 1084
TspDTI ATGAA 4 cut(s) 158, 353, 570, 765
Tth111I GACNNNGTC 1 cut(s) 472
VspI ATTAAT 1 cut(s) 552
XmnI GAANNNNTTC 1 cut(s) 726
XspI CTAG 3 cut(s) 201, 1041, 1049
ZrmI AGTACT 1 cut(s) 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.