Rh5BG189600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018702
Physical Location & Seq
Reverse (-)
20878557 .. 20880825
2269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG189600.1

Sequence Viewer

Length: 408 bp
ATGGTCCGCCAGAATTGTGAGCTCGGAGAGTTGTGGTTCGCCGTCTCCGATTCCAAGCATCGTCGCTACGCTAAGAAGAACTCCCAAGCTCTGTCGATGATGCTCGACCTCTGGAAGGACTTTTCGGCCAAGCTCCACCACAAGAGTAGCACAACAACCGCACTGAAATCGACGATCGATCGGAGACTCGTTGACGTCGTTGCAGCAGAAGTAGGCTTCCAGCAACGGGGTCTGATCAGATTTTTGGTCACCGCGCTCTTCGCTCATGAAACGCAGTCGTTGGACATTGATCCCTCCAAGCTCAAGGTCGGTATTCTATTCCGTCCTCATCATCGTCGAGTTCGGCTTCGTGGTGGCGACAATAATGTTGCAGAGCTCCATGACTTTGGTCTTCAGGACATTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

15.46

Weight (kDa)

9.69

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 198
AccII CGCG 1 cut(s) 254
AciI CCGC 3 cut(s) 7, 159, 252
AclWI GGATC 1 cut(s) 284
AcoI YGGCCR 1 cut(s) 126
AcuI CTGAAG 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 195
AfiI CCNNNNNNNGG 2 cut(s) 115, 226
AluBI AGCT 5 cut(s) 22, 89, 133, 301, 376
AluI AGCT 5 cut(s) 22, 89, 133, 301, 376
Alw21I GWGCWC 2 cut(s) 24, 378
Alw26I GTCTC 2 cut(s) 49, 178
AlwI GGATC 1 cut(s) 284
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 203
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 1 cut(s) 241
AvaII GGWCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 24, 378
BbsI GAAGAC 1 cut(s) 383
Bbv12I GWGCWC 2 cut(s) 24, 378
BbvI GCAGC 1 cut(s) 215
BceAI ACGGC 1 cut(s) 26
BcgI CGANNNNNNTGC 2 cut(s) 150, 184
BclI TGATCA 1 cut(s) 234
BcoDI GTCTC 2 cut(s) 49, 178
BisI GCNGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 205
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmsI GCATC 2 cut(s) 67, 90
BoxI GACNNNNGTC 1 cut(s) 387
BpiI GAAGAC 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 287
Bsa29I ATCGAT 1 cut(s) 177
BsaHI GRCGYC 1 cut(s) 195
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 226
BseCI ATCGAT 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 115, 226
BseXI GCAGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 254
Bsh1285I CGRYCG 2 cut(s) 177, 181
BshFI GGCC 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 177
BsiEI CGRYCG 2 cut(s) 177, 181
BsiHKAI GWGCWC 2 cut(s) 24, 378
BslI CCNNNNNNNGG 2 cut(s) 115, 226
BsmAI GTCTC 2 cut(s) 49, 178
BsmBI CGTCTC 1 cut(s) 49
BsnI GGCC 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 24, 378
Bsp143I GATC 4 cut(s) 174, 178, 234, 289
BspACI CCGC 3 cut(s) 7, 159, 252
BspANI GGCC 1 cut(s) 128
BspDI ATCGAT 1 cut(s) 177
BspFNI CGCG 1 cut(s) 254
BspHI TCATGA 1 cut(s) 265
BspPI GGATC 1 cut(s) 284
BspQI GCTCTTC 1 cut(s) 263
BssMI GATC 4 cut(s) 174, 178, 234, 289
BssNI GRCGYC 1 cut(s) 195
Bst6I CTCTTC 1 cut(s) 263
BstACI GRCGYC 1 cut(s) 195
BstDEI CTNAG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 113
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 4 cut(s) 177, 181, 237, 292
BstMAI GTCTC 2 cut(s) 49, 178
BstMBI GATC 4 cut(s) 174, 178, 234, 289
BstMCI CGRYCG 2 cut(s) 177, 181
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstPAI GACNNNNGTC 1 cut(s) 387
BstPI GGTNACC 1 cut(s) 247
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 1 cut(s) 215
BstV2I GAAGAC 1 cut(s) 383
BstXI CCANNNNNNTGG 1 cut(s) 386
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 1 cut(s) 128
BsuTUI ATCGAT 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 161
CciI TCATGA 1 cut(s) 265
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 177
CviAII CATG 2 cut(s) 266, 380
CviJI RGCY 8 cut(s) 22, 89, 128, 133, 216, 301, 346, 376
CviKI_1 RGCY 8 cut(s) 22, 89, 128, 133, 216, 301, 346, 376
DdeI CTNAG 1 cut(s) 72
DpnI GATC 4 cut(s) 176, 180, 236, 291
DpnII GATC 4 cut(s) 174, 178, 234, 289
EaeI YGGCCR 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Ecl136II GAGCTC 2 cut(s) 22, 376
Eco24I GRGCYC 2 cut(s) 24, 378
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 2 cut(s) 22, 376
Eco57I CTGAAG 1 cut(s) 377
Eco91I GGTNACC 1 cut(s) 247
EcoICRI GAGCTC 2 cut(s) 22, 376
EcoNI CCTNNNNNAGG 1 cut(s) 113
EcoO65I GGTNACC 1 cut(s) 247
EcoT38I GRGCYC 2 cut(s) 24, 378
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 2 cut(s) 269, 383
FaiI YATR 2 cut(s) 267, 381
FatI CATG 2 cut(s) 265, 379
FbaI TGATCA 1 cut(s) 234
Fnu4HI GCNGC 1 cut(s) 204
FriOI GRGCYC 2 cut(s) 24, 378
Fsp4HI GCNGC 1 cut(s) 204
GlaI GCGC 1 cut(s) 255
GluI GCNGC 1 cut(s) 204
HaeIII GGCC 1 cut(s) 128
HhaI GCGC 1 cut(s) 256
Hin1I GRCGYC 1 cut(s) 195
Hin1II CATG 2 cut(s) 269, 383
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 2 cut(s) 50, 186
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 5 cut(s) 26, 49, 183, 234, 239
Hpy188III TCNNGA 3 cut(s) 112, 266, 395
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 4 cut(s) 66, 175, 200, 339
HpyAV CCTTC 1 cut(s) 109
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 2 cut(s) 203, 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 1 cut(s) 72
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 195
Hsp92II CATG 2 cut(s) 269, 383
HspAI GCGC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 4 cut(s) 174, 178, 234, 289
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 2 cut(s) 138, 381
LpnPI CCDG 4 cut(s) 23, 97, 233, 380
Lsp1109I GCAGC 1 cut(s) 215
LweI GCATC 2 cut(s) 67, 90
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 247
MalI GATC 4 cut(s) 176, 180, 236, 291
MboI GATC 4 cut(s) 174, 178, 234, 289
MboII GAAGA 3 cut(s) 88, 250, 383
MhlI GDGCHC 2 cut(s) 24, 378
MluCI AATT 1 cut(s) 13
MlyI GAGTC 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 3 cut(s) 119, 304, 336
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 1 cut(s) 260
NdeII GATC 4 cut(s) 174, 178, 234, 289
NlaIII CATG 2 cut(s) 269, 383
NmuCI GTSAC 1 cut(s) 247
PagI TCATGA 1 cut(s) 265
PciSI GCTCTTC 1 cut(s) 263
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 195
PfeI GAWTC 1 cut(s) 50
PkrI GCNGC 1 cut(s) 205
Ple19I CGATCG 2 cut(s) 177, 181
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PshAI GACNNNNGTC 1 cut(s) 387
Psp124BI GAGCTC 2 cut(s) 24, 378
PspEI GGTNACC 1 cut(s) 247
PspPI GGNCC 1 cut(s) 4
PvuI CGATCG 2 cut(s) 177, 181
SacI GAGCTC 2 cut(s) 24, 378
SapI GCTCTTC 1 cut(s) 263
SatI GCNGC 1 cut(s) 204
Sau3AI GATC 4 cut(s) 174, 178, 234, 289
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 180
SduI GDGCHC 2 cut(s) 24, 378
SetI ASST 8 cut(s) 24, 91, 111, 135, 198, 303, 309, 378
SfaNI GCATC 2 cut(s) 67, 90
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 1 cut(s) 13
SsiI CCGC 3 cut(s) 7, 159, 252
SstI GAGCTC 2 cut(s) 24, 378
TaiI ACGT 1 cut(s) 198
TaqI TCGA 5 cut(s) 95, 105, 170, 177, 337
TasI AATT 1 cut(s) 13
TfiI GAWTC 1 cut(s) 50
TscAI CASTG 1 cut(s) 168
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 1 cut(s) 203
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 1 cut(s) 282
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 4
XagI CCTNNNNNAGG 1 cut(s) 113
ZraI GACGTC 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.