Rh5CG418400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0018702
Physical Location & Seq
Forward (+)
57311124 .. 57318209
7086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG418400.1

Sequence Viewer

Length: 363 bp
ATGTCGAGGTCATCGTCTCAGTCAACTCCGTCTGTGATGCCCGGCCTTTGGAAGGACCTTCCGGTCAAGCTCCACCACAAGGTCTACAGATTCTCAATGTCGCCGGCGTGTACCTCAATCTCGCTTCGAACACTTTCTCCTGTGAGATTCCGAGAAGCAGCGCCAGCCTTGGTGACAGATTTCAATCTAGAAAGGATTGGCGAAGGTGAGGACCATGGAGTTGGAGCCGACATCGTTGTTAGTGGCGCCGGAGACGAGTTTGCTGGAAGAAGCTGGAGATCCCTTGGTTCATTTGATTCATTTTTACTTGATCCTCCCGTGGTTCTTTCTTTGATTCATTTTTACTTGATCCTCAAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

13.16

Weight (kDa)

6.9

Isoelectric Point (pI)

72.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
AccB1I GGYRCC 1 cut(s) 245
AccI GTMKAC 1 cut(s) 84
AclWI GGATC 3 cut(s) 273, 305, 343
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 1 cut(s) 112
AfiI CCNNNNNNNGG 3 cut(s) 48, 52, 79
AgsI TTSAA 1 cut(s) 184
AloI GAACNNNNNNTCC 2 cut(s) 121, 153
AluBI AGCT 2 cut(s) 70, 273
AluI AGCT 2 cut(s) 70, 273
Alw26I GTCTC 2 cut(s) 21, 246
AlwI GGATC 3 cut(s) 273, 305, 343
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 133
AspLEI GCGC 2 cut(s) 163, 248
AspS9I GGNCC 2 cut(s) 55, 211
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 2 cut(s) 184, 218
AsuII TTCGAA 1 cut(s) 127
AvaII GGWCC 2 cut(s) 55, 211
BanI GGYRCC 1 cut(s) 245
BbvI GCAGC 1 cut(s) 170
BcnI CCSGG 1 cut(s) 42
BcoDI GTCTC 2 cut(s) 21, 246
BfaI CTAG 1 cut(s) 188
BfmI CTRYAG 1 cut(s) 85
BfoI RGCGCY 2 cut(s) 164, 249
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 2 cut(s) 55, 211
BmgT120I GGNCC 2 cut(s) 55, 211
BmiI GGNNCC 2 cut(s) 226, 247
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 295
Bpu14I TTCGAA 1 cut(s) 127
BpuEI CTTGAG 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 183
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 4 cut(s) 168, 214, 283, 318
BsaWI WCCGGW 1 cut(s) 61
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 52, 79
Bse118I RCCGGY 1 cut(s) 103
Bse8I GATNNNNATC 1 cut(s) 183
BseDI CCNNGG 4 cut(s) 168, 214, 283, 318
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 3 cut(s) 48, 52, 79
BseMII CTCAG 1 cut(s) 32
BseXI GCAGC 1 cut(s) 170
BshFI GGCC 1 cut(s) 45
BshNI GGYRCC 1 cut(s) 245
BsiSI CCGG 4 cut(s) 42, 62, 104, 249
BslI CCNNNNNNNGG 3 cut(s) 48, 52, 79
BsmAI GTCTC 2 cut(s) 21, 246
BsmBI CGTCTC 2 cut(s) 21, 246
BsnI GGCC 1 cut(s) 45
Bsp119I TTCGAA 1 cut(s) 127
Bsp143I GATC 3 cut(s) 278, 310, 348
Bsp19I CCATGG 1 cut(s) 214
BspANI GGCC 1 cut(s) 45
BspCNI CTCAG 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 226, 247
BspPI GGATC 3 cut(s) 273, 305, 343
BspT104I TTCGAA 1 cut(s) 127
BspT107I GGYRCC 1 cut(s) 245
BsrFI RCCGGY 1 cut(s) 103
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 4 cut(s) 168, 214, 283, 318
BssMI GATC 3 cut(s) 278, 310, 348
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 3 cut(s) 168, 214, 283
BstACI GRCGYC 1 cut(s) 246
BstBI TTCGAA 1 cut(s) 127
BstC8I GCNNGC 2 cut(s) 105, 165
BstDEI CTNAG 1 cut(s) 18
BstDSI CCRYGG 2 cut(s) 214, 318
BstENI CCTNNNNNAGG 1 cut(s) 50
BstH2I RGCGCY 2 cut(s) 164, 249
BstHHI GCGC 2 cut(s) 163, 248
BstKTI GATC 3 cut(s) 281, 313, 351
BstMAI GTCTC 2 cut(s) 21, 246
BstMBI GATC 3 cut(s) 278, 310, 348
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 40
BstSFI CTRYAG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 278
BstXI CCANNNNNNTGG 1 cut(s) 221
BstYI RGATCY 1 cut(s) 278
BsuRI GGCC 1 cut(s) 45
BtgI CCRYGG 2 cut(s) 214, 318
Cac8I GCNNGC 2 cut(s) 105, 165
CfoI GCGC 2 cut(s) 163, 248
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 55, 211
Csp6I GTAC 1 cut(s) 111
CviAII CATG 1 cut(s) 215
CviJI RGCY 5 cut(s) 45, 70, 167, 227, 273
CviKI_1 RGCY 5 cut(s) 45, 70, 167, 227, 273
CviQI GTAC 1 cut(s) 111
DdeI CTNAG 1 cut(s) 18
DinI GGCGCC 1 cut(s) 247
DpnI GATC 3 cut(s) 280, 312, 350
DpnII GATC 3 cut(s) 278, 310, 348
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
Eco130I CCWWGG 3 cut(s) 168, 214, 283
Eco47I GGWCC 2 cut(s) 55, 211
EcoNI CCTNNNNNAGG 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 55
EcoT14I CCWWGG 3 cut(s) 168, 214, 283
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
ErhI CCWWGG 3 cut(s) 168, 214, 283
Esp3I CGTCTC 2 cut(s) 21, 246
FaeI CATG 1 cut(s) 218
FaiI YATR 1 cut(s) 216
FatI CATG 1 cut(s) 214
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 188
GlaI GCGC 2 cut(s) 162, 247
GluI GCNGC 1 cut(s) 159
GsuI CTGGAG 1 cut(s) 295
HaeII RGCGCY 2 cut(s) 164, 249
HaeIII GGCC 1 cut(s) 45
HapII CCGG 4 cut(s) 42, 62, 104, 249
HhaI GCGC 2 cut(s) 163, 248
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 1 cut(s) 218
Hin6I GCGC 2 cut(s) 161, 246
HinP1I GCGC 2 cut(s) 161, 246
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 4 cut(s) 90, 147, 296, 334
HpaII CCGG 4 cut(s) 42, 62, 104, 249
HphI GGTGA 2 cut(s) 184, 218
Hpy166II GTNNAC 3 cut(s) 24, 85, 111
Hpy188I TCNGA 1 cut(s) 152
Hpy188III TCNNGA 1 cut(s) 188
Hpy8I GTNNAC 3 cut(s) 24, 85, 111
HpyAV CCTTC 3 cut(s) 46, 68, 197
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 1 cut(s) 218
HspAI GCGC 2 cut(s) 161, 246
KasI GGCGCC 1 cut(s) 245
KroI GCCGGC 1 cut(s) 103
KroNI GCCGGC 1 cut(s) 105
Kzo9I GATC 3 cut(s) 278, 310, 348
LmnI GCTCC 2 cut(s) 75, 224
LpnPI CCDG 8 cut(s) 55, 75, 117, 153, 177, 249, 259, 262
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 1 cut(s) 27
MaeI CTAG 1 cut(s) 188
MaeIII GTNAC 1 cut(s) 172
MalI GATC 3 cut(s) 280, 312, 350
MboI GATC 3 cut(s) 278, 310, 348
MboII GAAGA 1 cut(s) 279
MflI RGATCY 1 cut(s) 278
Mly113I GGCGCC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 4 cut(s) 124, 202, 324, 362
MreI CGCCGGCG 1 cut(s) 103
MroNI GCCGGC 1 cut(s) 103
MroXI GAANNNNTTC 1 cut(s) 133
MspI CCGG 4 cut(s) 42, 62, 104, 249
MspR9I CCNGG 1 cut(s) 42
MwoI GCNNNNNNNGC 1 cut(s) 164
NaeI GCCGGC 1 cut(s) 105
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 1 cut(s) 42
NcoI CCATGG 1 cut(s) 214
NdeII GATC 3 cut(s) 278, 310, 348
NgoMIV GCCGGC 1 cut(s) 103
NlaIII CATG 1 cut(s) 218
NlaIV GGNNCC 2 cut(s) 226, 247
NmuCI GTSAC 1 cut(s) 172
NspV TTCGAA 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PdiI GCCGGC 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 4 cut(s) 90, 147, 296, 334
PkrI GCNGC 1 cut(s) 160
PluTI GGCGCC 1 cut(s) 249
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 226, 247
PspPI GGNCC 2 cut(s) 55, 211
PspPPI RGGWCCY 1 cut(s) 55
PsuI RGATCY 1 cut(s) 278
RsaI GTAC 1 cut(s) 112
RsaNI GTAC 1 cut(s) 111
SatI GCNGC 1 cut(s) 159
Sau3AI GATC 3 cut(s) 278, 310, 348
Sau96I GGNCC 2 cut(s) 55, 211
ScrFI CCNGG 1 cut(s) 42
SetI ASST 8 cut(s) 11, 60, 72, 84, 116, 208, 275, 360
SfaNI GCATC 1 cut(s) 27
SfcI CTRYAG 1 cut(s) 85
SfoI GGCGCC 1 cut(s) 247
SfuI TTCGAA 1 cut(s) 127
SgrAI CRCCGGYG 1 cut(s) 103
SinI GGWCC 2 cut(s) 55, 211
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SspDI GGCGCC 1 cut(s) 245
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 40
StyI CCWWGG 3 cut(s) 168, 214, 283
TaqI TCGA 2 cut(s) 5, 127
TfiI GAWTC 4 cut(s) 90, 147, 296, 334
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 3 cut(s) 279, 288, 326
TspGWI ACGGA 1 cut(s) 18
VpaK11BI GGWCC 2 cut(s) 55, 211
XagI CCTNNNNNAGG 1 cut(s) 50
XbaI TCTAGA 1 cut(s) 187
XmiI GTMKAC 1 cut(s) 84
XmnI GAANNNNTTC 1 cut(s) 133
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.