Rh5BG386800

Protein-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003130
Physical Location & Seq
Forward (+)
61769014 .. 61773983
4970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG386800.1

Sequence Viewer

Length: 660 bp
ATGGAACCCGACAGGTTGAACTCGCCCAGCACATTTACAATGGATTTGGAAGTTTTGGGGCATGAGTTGCAGTTCATTCAGGATCCTAATTCCAAGCATCTAGGAACTACAGTTTGGGATGCATCACTGGTATTCGCCAAGTTTCTGGAAAAGAATTCCAGAAGGGGTAAGTTCTCCCCGTCAAAACTTAAAGGGAAACGTGTCATTGAACTTGGAGCAGGTTGTGGAGTAGCAGGGTTTGGCATGGCATTACTGGGATGTGATGTGGTTATGACAGATCAAGTTGAGGTTTTGCCATTGCTAATGAGAAATGTTGAACGGAATACTTCAAGGATTACACAGATGAATCCTGGTTCTGAGTCATTTGGTTCGGTCCAGGTAGCAGAGTTAAATTGGGGAAATGAGGATCATATAAGAGCCGTTGATCCGCCATTTGACTACATCATTGGCACTGACGTTGTTTACAAAGAAGATCTCTTGGAGCCACTGTTGCAGACAATATTTGCATTGTCAGGGCCTAAAACTGCAATTTTGCTGGGCTATGAGATTCGTTCTACAAGTGTCCATGAGCAAATGGTTGAAATGTGGAAAAGACACTTCGAGGTGAAACTTGTTCCAAATTCTAAGGTACAGTTTAGTTTTTCAGATGCAATGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

219

Amino Acids

24.55

Weight (kDa)

5.58

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 22 - 190 4.1e-33 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 209
AciI CCGC 1 cut(s) 428
AclWI GGATC 4 cut(s) 77, 90, 414, 419
AcsI RAATTY 2 cut(s) 154, 619
AfaI GTAC 1 cut(s) 630
AflIII ACRYGT 1 cut(s) 199
AgsI TTSAA 5 cut(s) 19, 209, 317, 330, 581
AjnI CCWGG 2 cut(s) 349, 375
AjuI GAANNNNNNNTTGG 2 cut(s) 97, 129
AlwI GGATC 4 cut(s) 77, 90, 414, 419
AoxI GGCC 1 cut(s) 515
ApoI RAATTY 2 cut(s) 154, 619
AspS9I GGNCC 2 cut(s) 373, 515
AsuHPI GGTGA 1 cut(s) 616
AvaII GGWCC 1 cut(s) 373
BamHI GGATCC 1 cut(s) 82
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 2 cut(s) 351, 377
BfaI CTAG 1 cut(s) 101
BfmI CTRYAG 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 209
BglII AGATCT 1 cut(s) 472
Bme1390I CCNGG 2 cut(s) 351, 377
Bme18I GGWCC 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 373, 515
BmiI GGNNCC 3 cut(s) 6, 84, 483
BmrFI CCNGG 2 cut(s) 351, 377
BmrI ACTGGG 1 cut(s) 263
BmsI GCATC 4 cut(s) 106, 109, 131, 637
BmuI ACTGGG 1 cut(s) 263
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bse1I ACTGG 2 cut(s) 132, 258
Bse3DI GCAATG 2 cut(s) 296, 657
BseBI CCWGG 2 cut(s) 351, 377
BseGI GGATG 2 cut(s) 124, 263
BseMI GCAATG 2 cut(s) 296, 657
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 2 cut(s) 132, 258
BseYI CCCAGC 2 cut(s) 26, 535
BshFI GGCC 1 cut(s) 517
BsnI GGCC 1 cut(s) 517
Bsp143I GATC 5 cut(s) 82, 277, 406, 424, 472
BspACI CCGC 1 cut(s) 428
BspANI GGCC 1 cut(s) 517
BspCNI CTCAG 1 cut(s) 349
BspLI GGNNCC 3 cut(s) 6, 84, 483
BspMI ACCTGC 1 cut(s) 209
BspPI GGATC 4 cut(s) 77, 90, 414, 419
BsrDI GCAATG 2 cut(s) 296, 657
BsrI ACTGG 2 cut(s) 132, 258
BssMI GATC 5 cut(s) 82, 277, 406, 424, 472
Bst2UI CCWGG 2 cut(s) 351, 377
Bst4CI ACNGT 3 cut(s) 112, 489, 633
BstAPI GCANNNNNTGC 1 cut(s) 67
BstDEI CTNAG 2 cut(s) 357, 624
BstF5I GGATG 2 cut(s) 124, 263
BstKTI GATC 5 cut(s) 85, 280, 409, 427, 475
BstMBI GATC 5 cut(s) 82, 277, 406, 424, 472
BstMWI GCNNNNNNNGC 2 cut(s) 67, 490
BstNI CCWGG 2 cut(s) 351, 377
BstSCI CCNGG 2 cut(s) 349, 375
BstSFI CTRYAG 1 cut(s) 108
BstX2I RGATCY 2 cut(s) 82, 472
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 2 cut(s) 82, 472
BsuRI GGCC 1 cut(s) 517
BtsCI GGATG 2 cut(s) 124, 263
BtsIMutI CAGTG 3 cut(s) 125, 450, 485
BveI ACCTGC 1 cut(s) 209
Cfr13I GGNCC 2 cut(s) 373, 515
Csp6I GTAC 1 cut(s) 629
CviAII CATG 3 cut(s) 62, 244, 566
CviJI RGCY 4 cut(s) 419, 484, 517, 540
CviKI_1 RGCY 4 cut(s) 419, 484, 517, 540
CviQI GTAC 1 cut(s) 629
DdeI CTNAG 2 cut(s) 357, 624
DpnI GATC 5 cut(s) 84, 279, 408, 426, 474
DpnII GATC 5 cut(s) 82, 277, 406, 424, 472
EciI GGCGGA 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 373
EcoO109I RGGNCCY 1 cut(s) 515
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 349, 375
EcoT22I ATGCAT 1 cut(s) 124
FaeI CATG 3 cut(s) 65, 247, 569
FaiI YATR 7 cut(s) 63, 245, 272, 411, 413, 543, 567
FatI CATG 3 cut(s) 61, 243, 565
FokI GGATG 2 cut(s) 131, 270
FspBI CTAG 1 cut(s) 101
GsaI CCCAGC 2 cut(s) 30, 539
HaeIII GGCC 1 cut(s) 517
Hin1II CATG 3 cut(s) 65, 247, 569
HinfI GANTC 3 cut(s) 346, 359, 547
HphI GGTGA 1 cut(s) 616
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 2 cut(s) 358, 646
Hpy188III TCNNGA 3 cut(s) 80, 146, 159
Hpy8I GTNNAC 1 cut(s) 463
HpyAV CCTTC 1 cut(s) 156
HpyCH4III ACNGT 3 cut(s) 112, 489, 633
HpyCH4IV ACGT 2 cut(s) 199, 456
HpyCH4V TGCA 6 cut(s) 70, 122, 493, 506, 527, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 490
HpyF3I CTNAG 2 cut(s) 357, 624
HpySE526I ACGT 2 cut(s) 199, 456
Hsp92II CATG 3 cut(s) 65, 247, 569
Kzo9I GATC 5 cut(s) 82, 277, 406, 424, 472
LmnI GCTCC 2 cut(s) 215, 481
LweI GCATC 4 cut(s) 106, 109, 131, 637
MaeI CTAG 1 cut(s) 101
MaeII ACGT 2 cut(s) 199, 456
MalI GATC 5 cut(s) 84, 279, 408, 426, 474
MboI GATC 5 cut(s) 82, 277, 406, 424, 472
MboII GAAGA 1 cut(s) 482
MflI RGATCY 2 cut(s) 82, 472
MluCI AATT 5 cut(s) 88, 154, 391, 528, 619
MlyI GAGTC 1 cut(s) 368
MnlI CCTC 3 cut(s) 280, 397, 595
Mph1103I ATGCAT 1 cut(s) 124
MseI TTAA 3 cut(s) 189, 389, 658
MspR9I CCNGG 2 cut(s) 351, 377
MvaI CCWGG 2 cut(s) 351, 377
MwoI GCNNNNNNNGC 2 cut(s) 67, 490
NdeII GATC 5 cut(s) 82, 277, 406, 424, 472
NlaIII CATG 3 cut(s) 65, 247, 569
NlaIV GGNNCC 3 cut(s) 6, 84, 483
NsiI ATGCAT 1 cut(s) 124
PfeI GAWTC 2 cut(s) 346, 547
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Psp6I CCWGG 2 cut(s) 349, 375
PspFI CCCAGC 2 cut(s) 26, 535
PspGI CCWGG 2 cut(s) 349, 375
PspN4I GGNNCC 3 cut(s) 6, 84, 483
PspPI GGNCC 2 cut(s) 373, 515
PsuI RGATCY 2 cut(s) 82, 472
RsaI GTAC 1 cut(s) 630
RsaNI GTAC 1 cut(s) 629
SaqAI TTAA 3 cut(s) 189, 389, 658
Sau3AI GATC 5 cut(s) 82, 277, 406, 424, 472
Sau96I GGNCC 2 cut(s) 373, 515
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 351, 377
SetI ASST 8 cut(s) 17, 202, 223, 291, 381, 459, 606, 630
SfaNI GCATC 4 cut(s) 106, 109, 131, 637
SfcI CTRYAG 1 cut(s) 108
SinI GGWCC 1 cut(s) 373
Sse9I AATT 5 cut(s) 88, 154, 391, 528, 619
SsiI CCGC 1 cut(s) 428
SspI AATATT 1 cut(s) 501
SspMI CTAG 1 cut(s) 101
StyD4I CCNGG 2 cut(s) 349, 375
TaaI ACNGT 3 cut(s) 112, 489, 633
TaiI ACGT 2 cut(s) 202, 459
TaqI TCGA 1 cut(s) 600
TaqII GACCGA 1 cut(s) 361
TasI AATT 5 cut(s) 88, 154, 391, 528, 619
TfiI GAWTC 2 cut(s) 346, 547
Tru1I TTAA 3 cut(s) 189, 389, 658
Tru9I TTAA 3 cut(s) 189, 389, 658
TscAI CASTG 3 cut(s) 132, 457, 492
TspDTI ATGAA 2 cut(s) 64, 359
TspGWI ACGGA 1 cut(s) 334
TspRI CASTG 3 cut(s) 132, 457, 492
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 2 cut(s) 154, 619
XspI CTAG 1 cut(s) 101
Zsp2I ATGCAT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.