Rw5G035500

Protein-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003130
Physical Location & Seq
Forward (+)
61464061 .. 61469312
5252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G035500.1

Sequence Viewer

Length: 990 bp
ATGGAACCCGACAGGTTGAACTCGCCCAGCACATTTACAATGGATTTGGAAGTTTTGGGGCATGAGTTGCAGTTCATTCAGGATCCTAATTCAAAGCATCTAGGAACTACAGTTTGGGATGCATCACTGGTATTCGCCAAGTTTTTGGAAAAGAATTCCAGAAGGGGTAAGTTCTCCCCGTCAAAACTTAAAGGGAAACGTGTCATTGAACTTGGAGCAGGTTGTGGAGTAGCAGGGTTTGGCATGGCATTACTGGGATGTGATGTGGTTATGACAGATCAAGTTGAGGTTTTGCCATTGCTAATGAGAAATGTTGAACGGAATACTTCAAGGATTACACAGATGAATCCTGGTTCTGAGTCATTTGGTTCGGTCCAGGTAGCAGAGTTAAATTGGGGAAATGAGGATCATATAAGAGCCGTTGATCCGCCATTTGACTACATCATTGGCACTGACGTTGTTTACAAAGAAGATCTCTTGGAGCCACTGTTGCAGACAATATTTGCATTGTCAGGGCCTAAAACTGCAATTTTGCTGGGCTATGAGATTCGTTCTACTAGTGTCCATGAGCAAATGGTTGAAATGTGGAAAAGACACTTCGAGGTGAAACTTGTTCCAAATTCTAAGATGGATAGTACCTACCAACACCCAAGTATTCAACTTTTCATTATGAGATCAAAGCCGCCATCAGGGTATGCAGAAAAAACAGTTGAGCAGATAGATCAAGAAGTTGATGAAGTGGAGACAGCAGACAATAAAGCCAAGGTAATTGATGAGAAAGTTGATGGAAATCCAGTTAAGGTGATTTATCAAAATGCTGTCGACGTGGGAAGCAGTAAAGAAAAAGGAGAAAATAGCTCTCTTTTAGGAAGTGCTGAAGAAGATTGTGAGCCAGTTACAATACCTCAGAATGGTAAACTTACTGATTGGGAAGCTAGAAGATATGGCTCGATGGCTGCACGTCTTCTGCACGATGTCAAAATCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

329

Amino Acids

36.67

Weight (kDa)

5.1

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 22 - 190 1.5e-32 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 209
AccI GTMKAC 1 cut(s) 822
AciI CCGC 2 cut(s) 428, 683
AclWI GGATC 4 cut(s) 77, 90, 414, 419
AcsI RAATTY 2 cut(s) 154, 619
AcuI CTGAAG 1 cut(s) 897
AfaI GTAC 1 cut(s) 637
AfiI CCNNNNNNNGG 2 cut(s) 689, 911
AflIII ACRYGT 1 cut(s) 199
AgsI TTSAA 7 cut(s) 19, 93, 209, 317, 330, 581, 659
AhlI ACTAGT 1 cut(s) 557
AjiI CACGTC 2 cut(s) 826, 962
AjnI CCWGG 2 cut(s) 349, 375
AjuI GAANNNNNNNTTGG 2 cut(s) 97, 129
AluBI AGCT 2 cut(s) 858, 935
AluI AGCT 2 cut(s) 858, 935
Alw26I GTCTC 1 cut(s) 737
AlwI GGATC 4 cut(s) 77, 90, 414, 419
AoxI GGCC 1 cut(s) 515
ApeKI GCWGC 1 cut(s) 956
ApoI RAATTY 2 cut(s) 154, 619
AspS9I GGNCC 2 cut(s) 373, 515
AsuHPI GGTGA 2 cut(s) 616, 814
AvaII GGWCC 1 cut(s) 373
BamHI GGATCC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 956
BbvI GCAGC 1 cut(s) 943
BccI CCATC 4 cut(s) 622, 694, 779, 946
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 2 cut(s) 351, 377
BcoDI GTCTC 1 cut(s) 737
BcuI ACTAGT 1 cut(s) 557
BfaI CTAG 3 cut(s) 101, 558, 936
BfmI CTRYAG 1 cut(s) 108
BfuAI ACCTGC 1 cut(s) 209
BglII AGATCT 1 cut(s) 472
BisI GCNGC 2 cut(s) 683, 957
BlsI GCNGC 2 cut(s) 684, 958
Bme1390I CCNGG 2 cut(s) 351, 377
Bme18I GGWCC 1 cut(s) 373
BmgBI CACGTC 2 cut(s) 826, 962
BmgT120I GGNCC 2 cut(s) 373, 515
BmiI GGNNCC 3 cut(s) 6, 84, 483
BmrFI CCNGG 2 cut(s) 351, 377
BmrI ACTGGG 1 cut(s) 263
BmsI GCATC 3 cut(s) 106, 109, 131
BmuI ACTGGG 1 cut(s) 263
BpiI GAAGAC 1 cut(s) 956
BsaBI GATNNNNATC 1 cut(s) 789
BsaJI CCNNGG 1 cut(s) 762
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bsc4I CCNNNNNNNGG 2 cut(s) 689, 911
Bse1I ACTGG 4 cut(s) 132, 258, 794, 893
Bse3DI GCAATG 1 cut(s) 296
Bse8I GATNNNNATC 1 cut(s) 789
BseBI CCWGG 2 cut(s) 351, 377
BseDI CCNNGG 1 cut(s) 762
BseGI GGATG 2 cut(s) 124, 263
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 2 cut(s) 689, 911
BseMI GCAATG 1 cut(s) 296
BseMII CTCAG 2 cut(s) 348, 920
BseNI ACTGG 4 cut(s) 132, 258, 794, 893
BseXI GCAGC 1 cut(s) 943
BseYI CCCAGC 2 cut(s) 26, 535
BsgI GTGCAG 2 cut(s) 942, 953
BshFI GGCC 1 cut(s) 517
BslI CCNNNNNNNGG 2 cut(s) 689, 911
BsmAI GTCTC 1 cut(s) 737
BsnI GGCC 1 cut(s) 517
Bsp143I GATC 7 cut(s) 82, 277, 406, 424, 472, 674, 721
BspACI CCGC 2 cut(s) 428, 683
BspANI GGCC 1 cut(s) 517
BspCNI CTCAG 2 cut(s) 349, 919
BspLI GGNNCC 3 cut(s) 6, 84, 483
BspMI ACCTGC 1 cut(s) 209
BspPI GGATC 4 cut(s) 77, 90, 414, 419
BsrDI GCAATG 1 cut(s) 296
BsrI ACTGG 4 cut(s) 132, 258, 794, 893
BssECI CCNNGG 1 cut(s) 762
BssMI GATC 7 cut(s) 82, 277, 406, 424, 472, 674, 721
BssT1I CCWWGG 1 cut(s) 762
Bst2UI CCWGG 2 cut(s) 351, 377
Bst4CI ACNGT 3 cut(s) 112, 489, 709
BstAPI GCANNNNNTGC 1 cut(s) 67
BstDEI CTNAG 3 cut(s) 357, 624, 906
BstF5I GGATG 2 cut(s) 124, 263
BstKTI GATC 7 cut(s) 85, 280, 409, 427, 475, 677, 724
BstMAI GTCTC 1 cut(s) 737
BstMBI GATC 7 cut(s) 82, 277, 406, 424, 472, 674, 721
BstMWI GCNNNNNNNGC 2 cut(s) 67, 490
BstNI CCWGG 2 cut(s) 351, 377
BstSCI CCNGG 2 cut(s) 349, 375
BstSFI CTRYAG 1 cut(s) 108
BstV1I GCAGC 1 cut(s) 943
BstV2I GAAGAC 1 cut(s) 956
BstX2I RGATCY 2 cut(s) 82, 472
BstXI CCANNNNNNTGG 1 cut(s) 145
BstYI RGATCY 2 cut(s) 82, 472
BsuRI GGCC 1 cut(s) 517
BtrI CACGTC 2 cut(s) 826, 962
BtsCI GGATG 2 cut(s) 124, 263
BtsIMutI CAGTG 3 cut(s) 125, 450, 485
BveI ACCTGC 1 cut(s) 209
Cfr13I GGNCC 2 cut(s) 373, 515
Csp6I GTAC 1 cut(s) 636
CviAII CATG 3 cut(s) 62, 244, 566
CviQI GTAC 1 cut(s) 636
DdeI CTNAG 3 cut(s) 357, 624, 906
DpnI GATC 7 cut(s) 84, 279, 408, 426, 474, 676, 723
DpnII GATC 7 cut(s) 82, 277, 406, 424, 472, 674, 721
EciI GGCGGA 1 cut(s) 417
Eco130I CCWWGG 1 cut(s) 762
Eco47I GGWCC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 897
EcoO109I RGGNCCY 1 cut(s) 515
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 349, 375
EcoT14I CCWWGG 1 cut(s) 762
EcoT22I ATGCAT 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 762
FaeI CATG 3 cut(s) 65, 247, 569
FatI CATG 3 cut(s) 61, 243, 565
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 2 cut(s) 683, 957
FokI GGATG 2 cut(s) 131, 270
Fsp4HI GCNGC 2 cut(s) 683, 957
FspBI CTAG 3 cut(s) 101, 558, 936
GluI GCNGC 2 cut(s) 683, 957
GsaI CCCAGC 2 cut(s) 30, 539
HaeIII GGCC 1 cut(s) 517
Hin1II CATG 3 cut(s) 65, 247, 569
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HinfI GANTC 3 cut(s) 346, 359, 547
HphI GGTGA 2 cut(s) 616, 814
Hpy166II GTNNAC 3 cut(s) 463, 823, 917
Hpy188I TCNGA 2 cut(s) 358, 909
Hpy188III TCNNGA 3 cut(s) 80, 159, 725
Hpy8I GTNNAC 3 cut(s) 463, 823, 917
Hpy99I CGWCG 1 cut(s) 827
HpyAV CCTTC 1 cut(s) 156
HpyCH4III ACNGT 3 cut(s) 112, 489, 709
HpyCH4IV ACGT 4 cut(s) 199, 456, 825, 961
HpyCH4V TGCA 8 cut(s) 70, 122, 493, 506, 527, 698, 959, 970
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 490
HpyF3I CTNAG 3 cut(s) 357, 624, 906
HpySE526I ACGT 4 cut(s) 199, 456, 825, 961
Hsp92II CATG 3 cut(s) 65, 247, 569
Kzo9I GATC 7 cut(s) 82, 277, 406, 424, 472, 674, 721
LmnI GCTCC 2 cut(s) 215, 481
Lsp1109I GCAGC 1 cut(s) 943
LweI GCATC 3 cut(s) 106, 109, 131
MaeI CTAG 3 cut(s) 101, 558, 936
MaeII ACGT 4 cut(s) 199, 456, 825, 961
MaeIII GTNAC 1 cut(s) 895
MalI GATC 7 cut(s) 84, 279, 408, 426, 474, 676, 723
MboI GATC 7 cut(s) 82, 277, 406, 424, 472, 674, 721
MboII GAAGA 5 cut(s) 482, 890, 893, 951, 956
MflI RGATCY 2 cut(s) 82, 472
MluCI AATT 6 cut(s) 88, 154, 391, 528, 619, 768
MlyI GAGTC 1 cut(s) 368
MnlI CCTC 4 cut(s) 280, 397, 595, 915
Mph1103I ATGCAT 1 cut(s) 124
MseI TTAA 3 cut(s) 189, 389, 798
MspR9I CCNGG 2 cut(s) 351, 377
MvaI CCWGG 2 cut(s) 351, 377
MwoI GCNNNNNNNGC 2 cut(s) 67, 490
NdeII GATC 7 cut(s) 82, 277, 406, 424, 472, 674, 721
NlaIII CATG 3 cut(s) 65, 247, 569
NlaIV GGNNCC 3 cut(s) 6, 84, 483
NsiI ATGCAT 1 cut(s) 124
PfeI GAWTC 2 cut(s) 346, 547
PkrI GCNGC 2 cut(s) 684, 958
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Psp6I CCWGG 2 cut(s) 349, 375
PspFI CCCAGC 2 cut(s) 26, 535
PspGI CCWGG 2 cut(s) 349, 375
PspN4I GGNNCC 3 cut(s) 6, 84, 483
PspPI GGNCC 2 cut(s) 373, 515
PsuI RGATCY 2 cut(s) 82, 472
RsaI GTAC 1 cut(s) 637
RsaNI GTAC 1 cut(s) 636
SalI GTCGAC 1 cut(s) 821
SaqAI TTAA 3 cut(s) 189, 389, 798
SatI GCNGC 2 cut(s) 683, 957
Sau3AI GATC 7 cut(s) 82, 277, 406, 424, 472, 674, 721
Sau96I GGNCC 2 cut(s) 373, 515
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 351, 377
SfaNI GCATC 3 cut(s) 106, 109, 131
SfcI CTRYAG 1 cut(s) 108
SinI GGWCC 1 cut(s) 373
SpeI ACTAGT 1 cut(s) 557
Sse9I AATT 6 cut(s) 88, 154, 391, 528, 619, 768
SsiI CCGC 2 cut(s) 428, 683
SspI AATATT 1 cut(s) 501
SspMI CTAG 3 cut(s) 101, 558, 936
StyD4I CCNGG 2 cut(s) 349, 375
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 3 cut(s) 112, 489, 709
TaiI ACGT 4 cut(s) 202, 459, 828, 964
TaqI TCGA 3 cut(s) 600, 822, 950
TaqII GACCGA 1 cut(s) 361
TasI AATT 6 cut(s) 88, 154, 391, 528, 619, 768
TauI GCSGC 1 cut(s) 685
TfiI GAWTC 2 cut(s) 346, 547
Tru1I TTAA 3 cut(s) 189, 389, 798
Tru9I TTAA 3 cut(s) 189, 389, 798
TscAI CASTG 3 cut(s) 132, 457, 492
TseI GCWGC 1 cut(s) 956
TspDTI ATGAA 4 cut(s) 64, 359, 655, 750
TspGWI ACGGA 1 cut(s) 334
TspRI CASTG 3 cut(s) 132, 457, 492
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 2 cut(s) 154, 619
XmiI GTMKAC 1 cut(s) 822
XspI CTAG 3 cut(s) 101, 558, 936
Zsp2I ATGCAT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.