Rh5BG545900

Nucleolar complex protein 2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008600
Physical Location & Seq
Reverse (-)
86798304 .. 86803509
5206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG545900.1

Sequence Viewer

Length: 1617 bp
ATGGAAGCAAAAAACCCAGTGAAAAAATCGAAACTTGGGAAAAGGAAAAGTGATGAGGGAACCCAGAAAAGCTCTGTAGGTCAAGCGAAACAGCATAAGGAACAACTCGAAAGACTTCATGAAAAGGACCCGGAGTTCTATGACTTCTTAAAAGAGCATGACCAGGAACTTCTGCAGTTTGACGATGAGGACATTGAAGAAGATTCGGATGCTAACTTGGAGGATGAAGAGACGCAAGAAGGTGATGAGATAGAAGAGGATGAAGACACTAGCGATCATGATGTCAAAAAGAAGGAAAAGGAGACGCCGAAAAAAGTTATAACTAGTGAAATGGTTGATTCTTGGTGCAACATGATTCGAGAAACTGGGAAATTGAGTGCTATTCATTCTCTCATGAAAGCTTTCCGCACTGCCTGCCACTATGGTGATGATAAAGAGGATGAGTCTACTCTAGATTTTAGTATTATGTCTAGCAGTGTCTTTAATAAAGTGATGGTATTTGTACTTAATGAAATGGATGGAATAATTCGGAACTTGTTGGAGCTCCCTGCCTTTGGTGGGAAAAAAGAGACCATAATAGATCTTATGAGCACAAAACGATGGAAGAACTACAACCATTTAGTGAAGTCATATCTTGGAAATGCTTTACATGTCTTGCGTCAAATGACTGACACAGACATGATATCATTTACTTTACGGCGTCTTAAACATTCCTCCATATTTTTGGCTGCTTTTCCGATTCTCCTGAGGAAGTATGTTAAGACTGCCCTTGATTTGTGGGGTTTAGGTGGAGGTGCCCTCCCAATTGCCTCCCTTCTATTTTTAAGAGATCTGTGCATTCGGCTGGGATCTGATTGCTTAGATGAATGCTTCAAGGGGATCTACAAAGCCTATGTCTTGAACTGCCAGTTTATGAATGCAGCAAAATTACAACATGTCCAGTTTCTTGGAAATTGTGTAATTGAACTTTACGGTGTAGACCTTCCGACTGCATATCAACATGCCTTCATTTTCATTCGGCAATTAGCAATGATCTTGCGAGAGGCACTTAGTTCAAAAACTAAGGAAGCATTTAGAAAGGTTTATGAGTGGAAGTTCATGAACTGCCTTGAGCTCTGGACTGGGGCTGTCTGTGCCTATGGCTCAGAAGCTGACTTTAGACCGGTTGTATATCCCCTGACCCAAATAATTTATGGGGCTGCCCGTCTAGTTCCAACAGCTAGGTATTTACCTCTCAGATTGCGTTGTATTAGAATGCTTAATCGAATTGCTGCTTCGACGGGTACTTTCACACCAGTTTCTATGTTGCTTTTGGACATGCTTGAAATGAAAGAATTGAATAGGCCCACCACAGGTGGTGTTGGCAAAGCGGTTGATTTGCGTACTATACTTAAGGTTAGCAAGCCAACCCTGAAGACCCGAGCATTTCAGGAGGCTTGTGTGTTGTCTGTTGTTGATGAGCTTGCTGAGCATTTAGCGCAGTGGAGCTATTCCATTGCTTTCCCTGAGTTGTCCTTTATACCAGGCATGCGATTGCGTGGCTTTTGCAAATCCACCAAGGTTGAGAGGTTTCGGAGAGCAACAAGAGAGCTTATCCGTCAGGGTGCTCATAGCTAA

Protein Analysis

538

Amino Acids

61.5

Weight (kDa)

6.9

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Noc2 PF03715 286 - 534 2.1e-92 Noc2p family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 320
AccB1I GGYRCC 1 cut(s) 794
AccI GTMKAC 2 cut(s) 446, 978
AciI CCGC 2 cut(s) 406, 1370
AclWI GGATC 2 cut(s) 856, 887
AcuI CTGAAG 1 cut(s) 1433
AcyI GRCGYC 2 cut(s) 305, 700
AfaI GTAC 3 cut(s) 504, 1285, 1384
AfiI CCNNNNNNNGG 3 cut(s) 554, 558, 1352
AflII CTTAAG 1 cut(s) 1391
AflIII ACRYGT 2 cut(s) 649, 934
AgeI ACCGGT 1 cut(s) 1162
AgsI TTSAA 7 cut(s) 197, 874, 901, 965, 1056, 1325, 1339
AhlI ACTAGT 1 cut(s) 323
AjnI CCWGG 2 cut(s) 162, 1522
AleI CACNNNNGTG 1 cut(s) 423
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
Alw21I GWGCWC 4 cut(s) 546, 593, 1116, 1609
Alw26I GTCTC 3 cut(s) 224, 296, 563
AlwI GGATC 2 cut(s) 856, 887
AlwNI CAGNNNCTG 1 cut(s) 1151
Ama87I CYCGRG 1 cut(s) 1419
AoxI GGCC 1 cut(s) 1343
ApeKI GCWGC 4 cut(s) 728, 920, 1199, 1271
ArsI GACNNNNNNTTYG 2 cut(s) 879, 911
AsiGI ACCGGT 1 cut(s) 1162
Asp700I GAANNNNTTC 2 cut(s) 114, 401
AspLEI GCGC 1 cut(s) 1480
AspS9I GGNCC 2 cut(s) 127, 1344
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 2 cut(s) 254, 437
AvaI CYCGRG 1 cut(s) 1419
AvaII GGWCC 1 cut(s) 127
AxyI CCTNAGG 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 799
BanI GGYRCC 1 cut(s) 794
BanII GRGCYC 2 cut(s) 546, 1116
BbsI GAAGAC 2 cut(s) 270, 1421
Bbv12I GWGCWC 4 cut(s) 546, 593, 1116, 1609
BbvI GCAGC 4 cut(s) 715, 932, 1186, 1258
BccI CCATC 3 cut(s) 487, 512, 594
BceAI ACGGC 1 cut(s) 713
BciT130I CCWGG 2 cut(s) 164, 1524
BcnI CCSGG 1 cut(s) 131
BcoDI GTCTC 3 cut(s) 224, 296, 563
BcuI ACTAGT 1 cut(s) 323
BfaI CTAG 6 cut(s) 270, 324, 452, 471, 1208, 1221
BfmI CTRYAG 2 cut(s) 75, 173
BfrI CTTAAG 1 cut(s) 1391
BglII AGATCT 2 cut(s) 580, 829
BisI GCNGC 4 cut(s) 729, 921, 1200, 1272
BlpI GCTNAGC 1 cut(s) 1467
BlsI GCNGC 4 cut(s) 730, 922, 1201, 1273
Bme1390I CCNGG 3 cut(s) 131, 164, 1524
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 1419
BmgT120I GGNCC 2 cut(s) 127, 1344
BmiI GGNNCC 3 cut(s) 61, 129, 796
BmrFI CCNGG 3 cut(s) 131, 164, 1524
BmrI ACTGGG 3 cut(s) 11, 375, 1131
BmsI GCATC 1 cut(s) 199
BmuI ACTGGG 3 cut(s) 11, 375, 1131
BpiI GAAGAC 2 cut(s) 270, 1421
BplI GAGNNNNNCTC 2 cut(s) 783, 815
Bpu1102I GCTNAGC 1 cut(s) 1467
BpuEI CTTGAG 1 cut(s) 1130
BpuMI CCSGG 1 cut(s) 131
BsaHI GRCGYC 2 cut(s) 305, 700
BsaI GGTCTC 1 cut(s) 563
BsaJI CCNNGG 1 cut(s) 1557
BsaWI WCCGGW 1 cut(s) 1162
Bsc4I CCNNNNNNNGG 3 cut(s) 554, 558, 1352
Bse118I RCCGGY 1 cut(s) 1162
Bse1I ACTGG 6 cut(s) 17, 370, 907, 940, 1126, 1295
Bse21I CCTNAGG 1 cut(s) 746
Bse3DI GCAATG 2 cut(s) 1035, 1494
BseBI CCWGG 2 cut(s) 164, 1524
BseDI CCNNGG 1 cut(s) 1557
BseGI GGATG 5 cut(s) 214, 229, 265, 445, 523
BseLI CCNNNNNNNGG 3 cut(s) 554, 558, 1352
BseMI GCAATG 2 cut(s) 1035, 1494
BseMII CTCAG 5 cut(s) 737, 1158, 1249, 1458, 1497
BseNI ACTGG 6 cut(s) 17, 370, 907, 940, 1126, 1295
BseSI GKGCMC 1 cut(s) 799
BseXI GCAGC 4 cut(s) 715, 932, 1186, 1258
BseYI CCCAGC 1 cut(s) 844
BshFI GGCC 1 cut(s) 1345
BshNI GGYRCC 1 cut(s) 794
BshTI ACCGGT 1 cut(s) 1162
BsiHKAI GWGCWC 4 cut(s) 546, 593, 1116, 1609
BsiHKCI CYCGRG 1 cut(s) 1419
BsiSI CCGG 2 cut(s) 131, 1163
BslI CCNNNNNNNGG 3 cut(s) 554, 558, 1352
BsmAI GTCTC 3 cut(s) 224, 296, 563
BsmBI CGTCTC 2 cut(s) 224, 296
BsmI GAATGC 4 cut(s) 837, 872, 922, 1260
BsnI GGCC 1 cut(s) 1345
Bso31I GGTCTC 1 cut(s) 563
BsoBI CYCGRG 1 cut(s) 1419
Bsp1286I GDGCHC 5 cut(s) 546, 593, 799, 1116, 1609
Bsp143I GATC 6 cut(s) 274, 580, 829, 848, 879, 1032
Bsp1720I GCTNAGC 1 cut(s) 1467
BspACI CCGC 2 cut(s) 406, 1370
BspANI GGCC 1 cut(s) 1345
BspCNI CTCAG 5 cut(s) 738, 1157, 1248, 1459, 1498
BspHI TCATGA 4 cut(s) 118, 277, 393, 1098
BspLI GGNNCC 3 cut(s) 61, 129, 796
BspMAI CTGCAG 1 cut(s) 177
BspPI GGATC 2 cut(s) 856, 887
BspT107I GGYRCC 1 cut(s) 794
BspTI CTTAAG 1 cut(s) 1391
BspTNI GGTCTC 1 cut(s) 563
BsrDI GCAATG 2 cut(s) 1035, 1494
BsrFI RCCGGY 1 cut(s) 1162
BsrI ACTGG 6 cut(s) 17, 370, 907, 940, 1126, 1295
BssAI RCCGGY 1 cut(s) 1162
BssECI CCNNGG 1 cut(s) 1557
BssMI GATC 6 cut(s) 274, 580, 829, 848, 879, 1032
BssNI GRCGYC 2 cut(s) 305, 700
BssT1I CCWWGG 1 cut(s) 1557
Bst2UI CCWGG 2 cut(s) 164, 1524
Bst4CI ACNGT 1 cut(s) 974
Bst6I CTCTTC 2 cut(s) 222, 249
BstACI GRCGYC 2 cut(s) 305, 700
BstAFI CTTAAG 1 cut(s) 1391
BstAPI GCANNNNNTGC 1 cut(s) 414
BstC8I GCNNGC 4 cut(s) 415, 1403, 1464, 1529
BstDEI CTNAG 8 cut(s) 746, 859, 1049, 1062, 1144, 1235, 1467, 1506
BstF5I GGATG 5 cut(s) 214, 229, 265, 445, 523
BstHHI GCGC 1 cut(s) 1480
BstKTI GATC 6 cut(s) 277, 583, 832, 851, 882, 1035
BstMAI GTCTC 3 cut(s) 224, 296, 563
BstMBI GATC 6 cut(s) 274, 580, 829, 848, 879, 1032
BstMWI GCNNNNNNNGC 4 cut(s) 414, 1133, 1468, 1477
BstNI CCWGG 2 cut(s) 164, 1524
BstNSI RCATGY 5 cut(s) 653, 938, 1004, 1321, 1531
BstSCI CCNGG 3 cut(s) 129, 162, 1522
BstSFI CTRYAG 2 cut(s) 75, 173
BstSLI GKGCMC 1 cut(s) 799
BstV1I GCAGC 4 cut(s) 715, 932, 1186, 1258
BstV2I GAAGAC 2 cut(s) 270, 1421
BstX2I RGATCY 4 cut(s) 580, 829, 848, 879
BstXI CCANNNNNNTGG 2 cut(s) 724, 947
BstYI RGATCY 4 cut(s) 580, 829, 848, 879
Bsu36I CCTNAGG 1 cut(s) 746
BsuRI GGCC 1 cut(s) 1345
BtsCI GGATG 5 cut(s) 214, 229, 265, 445, 523
BtsI GCAGTG 3 cut(s) 408, 481, 1487
BtsIMutI CAGTG 4 cut(s) 24, 408, 481, 1487
Cac8I GCNNGC 4 cut(s) 415, 1403, 1464, 1529
CaiI CAGNNNCTG 1 cut(s) 1151
CciI TCATGA 4 cut(s) 118, 277, 393, 1098
CfoI GCGC 1 cut(s) 1480
Cfr10I RCCGGY 1 cut(s) 1162
Cfr13I GGNCC 2 cut(s) 127, 1344
CseI GACGC 4 cut(s) 241, 313, 647, 689
Csp6I GTAC 3 cut(s) 503, 1284, 1383
CspAI ACCGGT 1 cut(s) 1162
CspCI CAANNNNNGTGG 2 cut(s) 1543, 1578
CviQI GTAC 3 cut(s) 503, 1284, 1383
DdeI CTNAG 8 cut(s) 746, 859, 1049, 1062, 1144, 1235, 1467, 1506
DpnI GATC 6 cut(s) 276, 582, 831, 850, 881, 1034
DpnII GATC 6 cut(s) 274, 580, 829, 848, 879, 1032
Eam1104I CTCTTC 2 cut(s) 222, 249
EarI CTCTTC 2 cut(s) 222, 249
Ecl136II GAGCTC 2 cut(s) 544, 1114
Eco130I CCWWGG 1 cut(s) 1557
Eco24I GRGCYC 2 cut(s) 546, 1116
Eco31I GGTCTC 1 cut(s) 563
Eco32I GATATC 1 cut(s) 684
Eco47I GGWCC 1 cut(s) 127
Eco53kI GAGCTC 2 cut(s) 544, 1114
Eco57I CTGAAG 1 cut(s) 1433
Eco81I CCTNAGG 1 cut(s) 746
Eco88I CYCGRG 1 cut(s) 1419
EcoICRI GAGCTC 2 cut(s) 544, 1114
EcoO109I RGGNCCY 1 cut(s) 127
EcoRII CCWGG 2 cut(s) 162, 1522
EcoRV GATATC 1 cut(s) 684
EcoT14I CCWWGG 1 cut(s) 1557
EcoT38I GRGCYC 2 cut(s) 546, 1116
ErhI CCWWGG 1 cut(s) 1557
Esp3I CGTCTC 2 cut(s) 224, 296
FblI GTMKAC 2 cut(s) 446, 978
Fnu4HI GCNGC 4 cut(s) 729, 921, 1200, 1272
FokI GGATG 5 cut(s) 221, 236, 272, 452, 530
FriOI GRGCYC 2 cut(s) 546, 1116
Fsp4HI GCNGC 4 cut(s) 729, 921, 1200, 1272
FspBI CTAG 6 cut(s) 270, 324, 452, 471, 1208, 1221
GlaI GCGC 1 cut(s) 1479
GluI GCNGC 4 cut(s) 729, 921, 1200, 1272
GsaI CCCAGC 1 cut(s) 848
HaeIII GGCC 1 cut(s) 1345
HapII CCGG 2 cut(s) 131, 1163
HgaI GACGC 4 cut(s) 241, 313, 647, 689
HhaI GCGC 1 cut(s) 1480
Hin1I GRCGYC 2 cut(s) 305, 700
Hin6I GCGC 1 cut(s) 1478
HinP1I GCGC 1 cut(s) 1478
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 5 cut(s) 203, 338, 355, 443, 739
HpaII CCGG 2 cut(s) 131, 1163
HphI GGTGA 2 cut(s) 254, 437
Hpy166II GTNNAC 2 cut(s) 447, 979
Hpy188I TCNGA 8 cut(s) 208, 531, 738, 853, 987, 1147, 1238, 1575
Hpy8I GTNNAC 2 cut(s) 447, 979
Hpy99I CGWCG 1 cut(s) 1282
HpyAV CCTTC 5 cut(s) 233, 286, 824, 992, 1015
HpyCH4III ACNGT 1 cut(s) 974
HpyCH4V TGCA 6 cut(s) 175, 348, 837, 920, 992, 1548
HpyF10VI GCNNNNNNNGC 4 cut(s) 414, 1133, 1468, 1477
HpyF3I CTNAG 8 cut(s) 746, 859, 1049, 1062, 1144, 1235, 1467, 1506
Hsp92I GRCGYC 2 cut(s) 305, 700
HspAI GCGC 1 cut(s) 1478
Kzo9I GATC 6 cut(s) 274, 580, 829, 848, 879, 1032
LmnI GCTCC 3 cut(s) 541, 549, 1485
Lsp1109I GCAGC 4 cut(s) 715, 932, 1186, 1258
LweI GCATC 1 cut(s) 199
MaeI CTAG 6 cut(s) 270, 324, 452, 471, 1208, 1221
MalI GATC 6 cut(s) 276, 582, 831, 850, 881, 1034
MboI GATC 6 cut(s) 274, 580, 829, 848, 879, 1032
MboII GAAGA 7 cut(s) 209, 212, 239, 266, 275, 616, 1426
MfeI CAATTG 1 cut(s) 804
MflI RGATCY 4 cut(s) 580, 829, 848, 879
MhlI GDGCHC 5 cut(s) 546, 593, 799, 1116, 1609
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 3 cut(s) 519, 1010, 1238
MroXI GAANNNNTTC 2 cut(s) 114, 401
MseI TTAA 8 cut(s) 149, 483, 507, 705, 759, 824, 1260, 1392
MslI CAYNNNNRTG 2 cut(s) 423, 677
MspCI CTTAAG 1 cut(s) 1391
MspI CCGG 2 cut(s) 131, 1163
MspR9I CCNGG 3 cut(s) 131, 164, 1524
MunI CAATTG 1 cut(s) 804
Mva1269I GAATGC 4 cut(s) 837, 872, 922, 1260
MvaI CCWGG 2 cut(s) 164, 1524
MwoI GCNNNNNNNGC 4 cut(s) 414, 1133, 1468, 1477
NciI CCSGG 1 cut(s) 131
NdeII GATC 6 cut(s) 274, 580, 829, 848, 879, 1032
NlaIV GGNNCC 3 cut(s) 61, 129, 796
NspI RCATGY 5 cut(s) 653, 938, 1004, 1321, 1531
OliI CACNNNNGTG 1 cut(s) 423
PaeI GCATGC 1 cut(s) 1531
PagI TCATGA 4 cut(s) 118, 277, 393, 1098
PciI ACATGT 2 cut(s) 649, 934
PctI GAATGC 4 cut(s) 837, 872, 922, 1260
PdmI GAANNNNTTC 2 cut(s) 114, 401
PfeI GAWTC 4 cut(s) 203, 338, 355, 739
PinAI ACCGGT 1 cut(s) 1162
PkrI GCNGC 4 cut(s) 730, 922, 1201, 1273
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PpuMI RGGWCCY 1 cut(s) 127
PscI ACATGT 2 cut(s) 649, 934
PsiI TTATAA 1 cut(s) 320
Psp124BI GAGCTC 2 cut(s) 546, 1116
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 162, 1522
PspFI CCCAGC 1 cut(s) 844
PspGI CCWGG 2 cut(s) 162, 1522
PspN4I GGNNCC 3 cut(s) 61, 129, 796
PspPI GGNCC 2 cut(s) 127, 1344
PspPPI RGGWCCY 1 cut(s) 127
PstI CTGCAG 1 cut(s) 177
PstNI CAGNNNCTG 1 cut(s) 1151
PsuI RGATCY 4 cut(s) 580, 829, 848, 879
RsaI GTAC 3 cut(s) 504, 1285, 1384
RsaNI GTAC 3 cut(s) 503, 1284, 1383
RseI CAYNNNNRTG 2 cut(s) 423, 677
SacI GAGCTC 2 cut(s) 546, 1116
SaqAI TTAA 8 cut(s) 149, 483, 507, 705, 759, 824, 1260, 1392
SatI GCNGC 4 cut(s) 729, 921, 1200, 1272
Sau3AI GATC 6 cut(s) 274, 580, 829, 848, 879, 1032
Sau96I GGNCC 2 cut(s) 127, 1344
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 3 cut(s) 131, 164, 1524
SduI GDGCHC 5 cut(s) 546, 593, 799, 1116, 1609
SfaNI GCATC 1 cut(s) 199
SfcI CTRYAG 2 cut(s) 75, 173
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 2 cut(s) 423, 677
SmlI CTYRAG 2 cut(s) 1109, 1391
SmoI CTYRAG 2 cut(s) 1109, 1391
SpeI ACTAGT 1 cut(s) 323
SphI GCATGC 1 cut(s) 1531
SsiI CCGC 2 cut(s) 406, 1370
SspMI CTAG 6 cut(s) 270, 324, 452, 471, 1208, 1221
SstI GAGCTC 2 cut(s) 546, 1116
StyD4I CCNGG 3 cut(s) 129, 162, 1522
StyI CCWWGG 1 cut(s) 1557
TaaI ACNGT 1 cut(s) 974
TaqI TCGA 5 cut(s) 29, 108, 358, 1264, 1277
TatI WGTACW 1 cut(s) 502
TfiI GAWTC 4 cut(s) 203, 338, 355, 739
Tru1I TTAA 8 cut(s) 149, 483, 507, 705, 759, 824, 1260, 1392
Tru9I TTAA 8 cut(s) 149, 483, 507, 705, 759, 824, 1260, 1392
TscAI CASTG 4 cut(s) 24, 415, 481, 1487
TseI GCWGC 4 cut(s) 728, 920, 1199, 1271
TspGWI ACGGA 1 cut(s) 1586
TspRI CASTG 4 cut(s) 24, 415, 481, 1487
Vha464I CTTAAG 1 cut(s) 1391
VpaK11BI GGWCC 1 cut(s) 127
XbaI TCTAGA 1 cut(s) 451
XceI RCATGY 5 cut(s) 653, 938, 1004, 1321, 1531
XcmI CCANNNNNNNNNTGG 1 cut(s) 1190
XmiI GTMKAC 2 cut(s) 446, 978
XmnI GAANNNNTTC 2 cut(s) 114, 401
XspI CTAG 6 cut(s) 270, 324, 452, 471, 1208, 1221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.