Rh5CG222800

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0014110
Physical Location & Seq
Reverse (-)
22687395 .. 22695976
8582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG222800.1

Sequence Viewer

Length: 1182 bp
ATGGAAGTTTATGAAACTGAAAACTCTGGATCTGCTGATCCAAAAAGTGATTCTGACAAAACCAAGGGAAAACGGAAATTATATGTAGGATCTCAAGCTTTAGGATATCGTCGGGATCATATGGAGGTGCTGCCCCCCATAAAGGATGGAGTTGTTGTTGATTGGGATGCTGTGGAGGGTATATGGGACCATGCTTTTAGGGACTGCCTATTGGTTGATCCCAAAGAGCATCCAATGCTACTTGCAGAGCCATCTTTTAACTCCCAGCAACAGAGAGAAAGGACGGCAGAGCTCATGTTTGAAAAATACAAAGTTCCTGCTTTATTCATGGCTAAGAATGCTGTTCTCACATCTTTTGCATCTGGCCGTGCTACCTCGTTGGTAGTTGACAGTGGCGGAGGATCAACTACAGTTGCTCCAGTTCATGATGGTTATGTTCTCCAAAAGGCTGTGTCATCCTCTCCTATTGGAGGAGAATTTCTTACTGATTGTTTGACAAAAAGTTTGGAAAGTAAAGGAATGACGATCAAACCCAGATATTCATTCAAGAGAAAGGAAACACGACCTGGAGAGTTTCAGGTTGTAGATGTTGATTTTCCAAACACCACAGAGAGCTACAAGCTCTATTCCAAGAGGATAATTGCTAGTGATATCAAGGAATGTGTGTGCAGAGCGCCTGACACTCCATATGATGAAAGCTCATATTCAAACATTCCAATGACCCCATATGAGCTTCCTGATGGCCAGACAATTGAAATTGGATCTGATAGATTCAAGATCCCTGATGTACTGTTTAATCCATCCTTAGCTCAGACGATTCCTGGTATGGAGAGTTTTGCAGAGATTGCTCCTTCTCTTCGTGGTTTACCTCAAATGGTTATAGACAGCATTAACAAGTGTGATGTGGACATCCGTCGTGAATTATTTAGTAGTATACTGCTTGCTGGTGGCACAGCATCGATGCAACAGTTAAAGGAACGCCTTGAGAAAGACTTGCTGGAGGAATCTCCCCAAGCTGCTAGAGTTAAAGTATTGGCAAGTGGAAATGCAACAGAAAGGCGATTCAGTGTTTGGATAGGTGGCAGTATATTGGCTTCTCTTGGTTCATTCCAACAGATGTGGTTCTCCAAGGCTGAGTACGAGGAGCATGGGGCTTCTTATGTACAAAGAAAGTGCCCTTGA

Protein Analysis

393

Amino Acids

43.66

Weight (kDa)

5.4

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 19 - 392 5.1e-144 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014110)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18450
fragaria_vesca FvH4_3g17390 FvH4_6g50670
malus_domestica MD03G1252000.v1.1 MD11G1273400.v1.1
prunus_persica Prupe.4G154200_v2.0.a1
pyrus_communis pycom03g19950
rosa_chinensis RchiOBHm_Chr5g0028921
rosa_laevigata RLG00000033127
rosa_multiflora Rmu_sc0005178.1_g000021
rosa_roxburghii Rroxscaffold_1G00050990
rosa_rugosa Rorug05G0111500
rosa_samantha Rh5AG203800 Rh5BG202100 Rh5CG222800 Rh5DG205400
rosa_wichuraiana Rw5G018450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 934
AciI CCGC 1 cut(s) 396
AclWI GGATC 8 cut(s) 32, 37, 97, 123, 212, 409, 769, 772
AcoI YGGCCR 2 cut(s) 364, 742
AcsI RAATTY 1 cut(s) 476
AfaI GTAC 3 cut(s) 789, 1139, 1164
AfiI CCNNNNNNNGG 2 cut(s) 142, 470
AgsI TTSAA 5 cut(s) 302, 547, 708, 755, 775
AjnI CCWGG 2 cut(s) 565, 820
AjuI GAANNNNNNNTTGG 2 cut(s) 34, 66
AluBI AGCT 8 cut(s) 98, 292, 615, 622, 699, 733, 809, 1016
AluI AGCT 8 cut(s) 98, 292, 615, 622, 699, 733, 809, 1016
Alw21I GWGCWC 1 cut(s) 294
AlwI GGATC 8 cut(s) 32, 37, 97, 123, 212, 409, 769, 772
AoxI GGCC 2 cut(s) 364, 742
ApeKI GCWGC 2 cut(s) 130, 1016
ApoI RAATTY 1 cut(s) 476
ArsI GACNNNNNNTTYG 2 cut(s) 487, 519
AspLEI GCGC 1 cut(s) 676
AspS9I GGNCC 1 cut(s) 187
AvaII GGWCC 1 cut(s) 187
BaeGI GKGCMC 1 cut(s) 1178
BalI TGGCCA 1 cut(s) 744
BanII GRGCYC 1 cut(s) 294
BarI GAAGNNNNNNTAC 2 cut(s) 1078, 1110
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 2 cut(s) 117, 1003
BccI CCATC 5 cut(s) 140, 259, 422, 734, 808
BceAI ACGGC 2 cut(s) 300, 351
BciT130I CCWGG 2 cut(s) 567, 822
BfaI CTAG 2 cut(s) 645, 1020
BfmI CTRYAG 1 cut(s) 408
BfoI RGCGCY 1 cut(s) 677
BisI GCNGC 2 cut(s) 131, 1017
BlsI GCNGC 2 cut(s) 132, 1018
Bme1390I CCNGG 2 cut(s) 567, 822
Bme18I GGWCC 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 188
BmrFI CCNGG 2 cut(s) 567, 822
BmsI GCATC 5 cut(s) 157, 238, 368, 951, 965
BoxI GACNNNNGTC 1 cut(s) 912
BpmI CTGGAG 3 cut(s) 402, 588, 1019
Bpu10I CCTNAGC 1 cut(s) 805
BpuEI CTTGAG 2 cut(s) 78, 1004
Bsa29I ATCGAT 1 cut(s) 959
BsaJI CCNNGG 2 cut(s) 63, 1128
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 400, 430
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 470
Bse1I ACTGG 1 cut(s) 419
BseBI CCWGG 2 cut(s) 567, 822
BseCI ATCGAT 1 cut(s) 959
BseDI CCNNGG 2 cut(s) 63, 1128
BseGI GGATG 6 cut(s) 151, 172, 229, 455, 800, 909
BseLI CCNNNNNNNGG 2 cut(s) 142, 470
BseMII CTCAG 2 cut(s) 824, 1125
BseNI ACTGG 1 cut(s) 419
BseRI GAGGAG 2 cut(s) 486, 1157
BseSI GKGCMC 1 cut(s) 1178
BseXI GCAGC 2 cut(s) 117, 1003
BseYI CCCAGC 1 cut(s) 264
BsgI GTGCAG 1 cut(s) 688
BshFI GGCC 2 cut(s) 366, 744
BshVI ATCGAT 1 cut(s) 959
BsiHKAI GWGCWC 1 cut(s) 294
BslFI GGGAC 2 cut(s) 200, 215
BslI CCNNNNNNNGG 2 cut(s) 142, 470
BsmFI GGGAC 2 cut(s) 200, 215
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 2 cut(s) 366, 744
Bsp1286I GDGCHC 2 cut(s) 294, 1178
Bsp1407I TGTACA 1 cut(s) 1162
Bsp143I GATC 9 cut(s) 29, 37, 89, 115, 217, 401, 525, 761, 777
BspACI CCGC 1 cut(s) 396
BspANI GGCC 2 cut(s) 366, 744
BspCNI CTCAG 2 cut(s) 823, 1126
BspDI ATCGAT 1 cut(s) 959
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 188
BspPI GGATC 8 cut(s) 32, 37, 97, 123, 212, 409, 769, 772
BsrGI TGTACA 1 cut(s) 1162
BsrI ACTGG 1 cut(s) 419
BssECI CCNNGG 2 cut(s) 63, 1128
BssMI GATC 9 cut(s) 29, 37, 89, 115, 217, 401, 525, 761, 777
BssNAI GTATAC 1 cut(s) 935
BssT1I CCWWGG 2 cut(s) 63, 1128
Bst1107I GTATAC 1 cut(s) 935
Bst2UI CCWGG 2 cut(s) 567, 822
Bst4CI ACNGT 4 cut(s) 392, 412, 792, 969
Bst6I CTCTTC 1 cut(s) 861
BstAPI GCANNNNNTGC 2 cut(s) 235, 845
BstAUI TGTACA 1 cut(s) 1162
BstC8I GCNNGC 1 cut(s) 942
BstDEI CTNAG 4 cut(s) 333, 805, 810, 1134
BstENI CCTNNNNNAGG 1 cut(s) 468
BstF5I GGATG 6 cut(s) 151, 172, 229, 455, 800, 909
BstH2I RGCGCY 1 cut(s) 677
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 9 cut(s) 32, 40, 92, 118, 220, 404, 528, 764, 780
BstMBI GATC 9 cut(s) 29, 37, 89, 115, 217, 401, 525, 761, 777
BstMWI GCNNNNNNNGC 3 cut(s) 235, 338, 845
BstNI CCWGG 2 cut(s) 567, 822
BstPAI GACNNNNGTC 1 cut(s) 912
BstSCI CCNGG 2 cut(s) 565, 820
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 1178
BstV1I GCAGC 2 cut(s) 117, 1003
BstX2I RGATCY 4 cut(s) 29, 89, 761, 777
BstYI RGATCY 4 cut(s) 29, 89, 761, 777
BstZ17I GTATAC 1 cut(s) 935
Bsu15I ATCGAT 1 cut(s) 959
BsuRI GGCC 2 cut(s) 366, 744
BsuTUI ATCGAT 1 cut(s) 959
BtsCI GGATG 6 cut(s) 151, 172, 229, 455, 800, 909
BtsIMutI CAGTG 2 cut(s) 397, 1072
Cac8I GCNNGC 1 cut(s) 942
CciI TCATGA 1 cut(s) 424
CfoI GCGC 1 cut(s) 676
Cfr13I GGNCC 1 cut(s) 187
ClaI ATCGAT 1 cut(s) 959
Csp6I GTAC 3 cut(s) 788, 1138, 1163
CspCI CAANNNNNGTGG 2 cut(s) 1100, 1135
CviAII CATG 5 cut(s) 191, 295, 328, 425, 1148
CviQI GTAC 3 cut(s) 788, 1138, 1163
DdeI CTNAG 4 cut(s) 333, 805, 810, 1134
DpnI GATC 9 cut(s) 31, 39, 91, 117, 219, 403, 527, 763, 779
DpnII GATC 9 cut(s) 29, 37, 89, 115, 217, 401, 525, 761, 777
EaeI YGGCCR 2 cut(s) 364, 742
Eam1104I CTCTTC 1 cut(s) 861
EarI CTCTTC 1 cut(s) 861
EciI GGCGGA 1 cut(s) 411
Ecl136II GAGCTC 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 63, 1128
Eco24I GRGCYC 1 cut(s) 294
Eco32I GATATC 2 cut(s) 107, 652
Eco47I GGWCC 1 cut(s) 187
Eco53kI GAGCTC 1 cut(s) 292
EcoICRI GAGCTC 1 cut(s) 292
EcoNI CCTNNNNNAGG 1 cut(s) 468
EcoRII CCWGG 2 cut(s) 565, 820
EcoRV GATATC 2 cut(s) 107, 652
EcoT14I CCWWGG 2 cut(s) 63, 1128
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 2 cut(s) 63, 1128
FaeI CATG 5 cut(s) 194, 298, 331, 428, 1151
FaqI GGGAC 2 cut(s) 200, 215
FatI CATG 5 cut(s) 190, 294, 327, 424, 1147
FauNDI CATATG 3 cut(s) 120, 688, 727
FblI GTMKAC 1 cut(s) 934
Fnu4HI GCNGC 2 cut(s) 131, 1017
FokI GGATG 6 cut(s) 158, 179, 216, 442, 787, 896
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 2 cut(s) 131, 1017
FspBI CTAG 2 cut(s) 645, 1020
GlaI GCGC 1 cut(s) 675
GluI GCNGC 2 cut(s) 131, 1017
GsaI CCCAGC 1 cut(s) 268
GsuI CTGGAG 3 cut(s) 402, 588, 1019
HaeII RGCGCY 1 cut(s) 677
HaeIII GGCC 2 cut(s) 366, 744
HhaI GCGC 1 cut(s) 676
Hin1II CATG 5 cut(s) 194, 298, 331, 428, 1151
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 5 cut(s) 50, 771, 817, 1004, 1062
Hpy166II GTNNAC 4 cut(s) 388, 866, 907, 935
Hpy188I TCNGA 3 cut(s) 55, 766, 813
Hpy188III TCNNGA 7 cut(s) 27, 113, 425, 547, 737, 775, 917
Hpy8I GTNNAC 4 cut(s) 388, 866, 907, 935
Hpy99I CGWCG 2 cut(s) 114, 918
HpyAV CCTTC 1 cut(s) 861
HpyCH4III ACNGT 4 cut(s) 392, 412, 792, 969
HpyCH4V TGCA 6 cut(s) 245, 359, 669, 839, 964, 1049
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 338, 845
HpyF3I CTNAG 4 cut(s) 333, 805, 810, 1134
Hsp92II CATG 5 cut(s) 194, 298, 331, 428, 1151
HspAI GCGC 1 cut(s) 674
Kzo9I GATC 9 cut(s) 29, 37, 89, 115, 217, 401, 525, 761, 777
LmnI GCTCC 3 cut(s) 421, 853, 1144
Lsp1109I GCAGC 2 cut(s) 117, 1003
LweI GCATC 5 cut(s) 157, 238, 368, 951, 965
MaeI CTAG 2 cut(s) 645, 1020
MalI GATC 9 cut(s) 31, 39, 91, 117, 219, 403, 527, 763, 779
MboI GATC 9 cut(s) 29, 37, 89, 115, 217, 401, 525, 761, 777
MboII GAAGA 1 cut(s) 848
MfeI CAATTG 1 cut(s) 750
MflI RGATCY 4 cut(s) 29, 89, 761, 777
MhlI GDGCHC 2 cut(s) 294, 1178
MlsI TGGCCA 1 cut(s) 744
MluCI AATT 6 cut(s) 77, 476, 639, 750, 756, 920
MluNI TGGCCA 1 cut(s) 744
MmeI TCCRAC 1 cut(s) 1135
Mox20I TGGCCA 1 cut(s) 744
MscI TGGCCA 1 cut(s) 744
MseI TTAA 5 cut(s) 258, 795, 891, 971, 1026
MslI CAYNNNNRTG 1 cut(s) 716
Msp20I TGGCCA 1 cut(s) 744
MspR9I CCNGG 2 cut(s) 567, 822
MunI CAATTG 1 cut(s) 750
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 2 cut(s) 567, 822
MwoI GCNNNNNNNGC 3 cut(s) 235, 338, 845
NdeI CATATG 3 cut(s) 120, 688, 727
NdeII GATC 9 cut(s) 29, 37, 89, 115, 217, 401, 525, 761, 777
NlaIII CATG 5 cut(s) 194, 298, 331, 428, 1151
NlaIV GGNNCC 1 cut(s) 188
PagI TCATGA 1 cut(s) 424
PctI GAATGC 1 cut(s) 343
PfeI GAWTC 5 cut(s) 50, 771, 817, 1004, 1062
PkrI GCNGC 2 cut(s) 132, 1018
PshAI GACNNNNGTC 1 cut(s) 912
Psp124BI GAGCTC 1 cut(s) 294
Psp6I CCWGG 2 cut(s) 565, 820
PspFI CCCAGC 1 cut(s) 264
PspGI CCWGG 2 cut(s) 565, 820
PspN4I GGNNCC 1 cut(s) 188
PspPI GGNCC 1 cut(s) 187
PsuI RGATCY 4 cut(s) 29, 89, 761, 777
RsaI GTAC 3 cut(s) 789, 1139, 1164
RsaNI GTAC 3 cut(s) 788, 1138, 1163
RseI CAYNNNNRTG 1 cut(s) 716
SacI GAGCTC 1 cut(s) 294
SaqAI TTAA 5 cut(s) 258, 795, 891, 971, 1026
SatI GCNGC 2 cut(s) 131, 1017
Sau3AI GATC 9 cut(s) 29, 37, 89, 115, 217, 401, 525, 761, 777
Sau96I GGNCC 1 cut(s) 187
ScrFI CCNGG 2 cut(s) 567, 822
SduI GDGCHC 2 cut(s) 294, 1178
SfaNI GCATC 5 cut(s) 157, 238, 368, 951, 965
SfcI CTRYAG 1 cut(s) 408
SinI GGWCC 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 716
SmlI CTYRAG 2 cut(s) 93, 983
SmoI CTYRAG 2 cut(s) 93, 983
Sse9I AATT 6 cut(s) 77, 476, 639, 750, 756, 920
SsiI CCGC 1 cut(s) 396
SspMI CTAG 2 cut(s) 645, 1020
SstI GAGCTC 1 cut(s) 294
StyD4I CCNGG 2 cut(s) 565, 820
StyI CCWWGG 2 cut(s) 63, 1128
TaaI ACNGT 4 cut(s) 392, 412, 792, 969
TaqI TCGA 1 cut(s) 959
TasI AATT 6 cut(s) 77, 476, 639, 750, 756, 920
TatI WGTACW 2 cut(s) 787, 1162
TfiI GAWTC 5 cut(s) 50, 771, 817, 1004, 1062
Tru1I TTAA 5 cut(s) 258, 795, 891, 971, 1026
Tru9I TTAA 5 cut(s) 258, 795, 891, 971, 1026
TscAI CASTG 2 cut(s) 397, 1072
TseI GCWGC 2 cut(s) 130, 1016
TspDTI ATGAA 6 cut(s) 27, 316, 413, 531, 708, 1095
TspGWI ACGGA 2 cut(s) 88, 902
TspRI CASTG 2 cut(s) 397, 1072
VpaK11BI GGWCC 1 cut(s) 187
XagI CCTNNNNNAGG 1 cut(s) 468
XapI RAATTY 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 934
XspI CTAG 2 cut(s) 645, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.