Rh5DG035600

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010695
Physical Location & Seq
Forward (+)
2524036 .. 2525143
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG035600.1

Sequence Viewer

Length: 828 bp
ATGGCGGTAGCACCTTCACTTGCTGGTCCGTTGCTCAGAATGCATTTCCATGATTGCTTTGTCAGGGGTTGCGACGGTTCAGTGCTATTGAATTCTTCAACAAACCAAGCCGAAAAGGATGCACCTCCCAACCTAAGTCTTCGAGGGTATGGAGTCATTGACAGTGTTAAGTCTGCTTTGGAAAAAGCTTGTCCTGGAGTAGTTTCATGCTCTGACATCGTAGCCGTTGTAGCTAGGGATGTGGTTGTCGCGGACATGGGAGGACACTGGGATGTTGAAACTGGACGAAGAGATGGTAATGTATCAAGCATGACAGATGCCTTGCTCAATCTCCCATCACCTCGTTCAAACATCACTATACTAAAATCAAGCTTTGCACAGAAGGGTCTAAGCGCTAAAGATCTTGTAGTACTATCAGGGAGTCACACCATTGGGACGTCTCATTGCTCTTCCTTCAACAATCGTCTTTACAACTTCACCGGAAATGGCGATACCGATCCCACCTTGGATAACAACTACATTGCAAGGTTGAAGATGAAATGCAAGCCCAACGACCAAACAACTCTCGCTGAGATGGATCCGGGGAGTTTCAAGACATTTGACGTCTCCTATTATACTCTTGTGGCCAAGAGAAGGGGTCTCTTTCAGTCAGATGCAGCTCTTCTTGATGACAGTGAGACCAAAACTTACGTTACAAACCATGCCACACCTAAGGGAAAAGCTAGTTTCTTGAAGGATTTTGGTGTTTCAATGGTGAAAATGGGTAGGATCGGAGTTCTCACGGGGAATGCAGGGGAGATCAGGCGAGTCTGCAGCAAGATTAATTAA

Protein Analysis

275

Amino Acids

29.5

Weight (kDa)

8.63

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 5 - 235 3.9e-81 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 440, 606
AccII CGCG 1 cut(s) 251
AciI CCGC 2 cut(s) 5, 251
AclWI GGATC 4 cut(s) 491, 572, 585, 776
AcoI YGGCCR 1 cut(s) 624
AcsI RAATTY 1 cut(s) 91
AcyI GRCGYC 2 cut(s) 437, 603
AfaI GTAC 1 cut(s) 411
AfeI AGCGCT 1 cut(s) 394
AfiI CCNNNNNNNGG 1 cut(s) 633
AgsI TTSAA 9 cut(s) 91, 99, 278, 348, 457, 532, 592, 733, 750
AjnI CCWGG 1 cut(s) 193
AluBI AGCT 5 cut(s) 188, 233, 372, 659, 722
AluI AGCT 5 cut(s) 188, 233, 372, 659, 722
Alw26I GTCTC 4 cut(s) 444, 610, 644, 671
AlwI GGATC 4 cut(s) 491, 572, 585, 776
Aor51HI AGCGCT 1 cut(s) 394
AoxI GGCC 1 cut(s) 624
ApeKI GCWGC 2 cut(s) 656, 813
ApoI RAATTY 1 cut(s) 91
AseI ATTAAT 1 cut(s) 822
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 1 cut(s) 26
AsuC2I CCSGG 1 cut(s) 582
AsuHPI GGTGA 3 cut(s) 330, 469, 766
AvaII GGWCC 1 cut(s) 26
AxyI CCTNAGG 1 cut(s) 711
BalI TGGCCA 1 cut(s) 626
BamHI GGATCC 1 cut(s) 577
BbsI GAAGAC 1 cut(s) 131
BbvI GCAGC 1 cut(s) 668
BccI CCATC 3 cut(s) 287, 343, 568
BceAI ACGGC 1 cut(s) 209
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 1 cut(s) 195
BcnI CCSGG 1 cut(s) 582
BcoDI GTCTC 4 cut(s) 444, 610, 644, 671
BfaI CTAG 2 cut(s) 234, 723
BfmI CTRYAG 1 cut(s) 811
BfoI RGCGCY 1 cut(s) 396
BglII AGATCT 1 cut(s) 400
BisI GCNGC 2 cut(s) 657, 814
BlsI GCNGC 2 cut(s) 658, 815
BmcAI AGTACT 1 cut(s) 411
Bme1390I CCNGG 2 cut(s) 195, 582
Bme18I GGWCC 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 579
BmrFI CCNGG 2 cut(s) 195, 582
BmrI ACTGGG 1 cut(s) 277
BmsI GCATC 3 cut(s) 109, 307, 643
BmuI ACTGGG 1 cut(s) 277
BpiI GAAGAC 1 cut(s) 131
BpmI CTGGAG 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 582
BsaBI GATNNNNATC 1 cut(s) 495
BsaHI GRCGYC 2 cut(s) 437, 603
BsaI GGTCTC 2 cut(s) 644, 671
BsaJI CCNNGG 2 cut(s) 504, 581
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 1 cut(s) 633
Bse1I ACTGG 2 cut(s) 272, 286
Bse21I CCTNAGG 1 cut(s) 711
Bse3DI GCAATG 2 cut(s) 442, 519
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 2 cut(s) 504, 581
BseGI GGATG 3 cut(s) 124, 244, 277
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 633
BseMI GCAATG 2 cut(s) 442, 519
BseMII CTCAG 2 cut(s) 49, 561
BseNI ACTGG 2 cut(s) 272, 286
BseXI GCAGC 1 cut(s) 668
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 1 cut(s) 626
BsiSI CCGG 2 cut(s) 480, 581
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 1 cut(s) 633
BsmAI GTCTC 4 cut(s) 444, 610, 644, 671
BsmBI CGTCTC 2 cut(s) 444, 610
BsmFI GGGAC 1 cut(s) 448
BsmI GAATGC 2 cut(s) 45, 793
BsnI GGCC 1 cut(s) 626
Bso31I GGTCTC 2 cut(s) 644, 671
Bsp143I GATC 5 cut(s) 400, 496, 577, 768, 798
BspACI CCGC 2 cut(s) 5, 251
BspANI GGCC 1 cut(s) 626
BspCNI CTCAG 2 cut(s) 48, 562
BspFNI CGCG 1 cut(s) 251
BspLI GGNNCC 1 cut(s) 579
BspMAI CTGCAG 1 cut(s) 815
BspPI GGATC 4 cut(s) 491, 572, 585, 776
BspQI GCTCTTC 2 cut(s) 454, 666
BspTNI GGTCTC 2 cut(s) 644, 671
BsrDI GCAATG 2 cut(s) 442, 519
BsrI ACTGG 2 cut(s) 272, 286
BssECI CCNNGG 2 cut(s) 504, 581
BssMI GATC 5 cut(s) 400, 496, 577, 768, 798
BssNI GRCGYC 2 cut(s) 437, 603
BssT1I CCWWGG 1 cut(s) 504
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 3 cut(s) 77, 164, 674
Bst6I CTCTTC 3 cut(s) 283, 454, 666
BstACI GRCGYC 2 cut(s) 437, 603
BstC8I GCNNGC 1 cut(s) 545
BstDEI CTNAG 5 cut(s) 35, 134, 389, 570, 711
BstF5I GGATG 3 cut(s) 124, 244, 277
BstFNI CGCG 1 cut(s) 251
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 5 cut(s) 403, 499, 580, 771, 801
BstMAI GTCTC 4 cut(s) 444, 610, 644, 671
BstMBI GATC 5 cut(s) 400, 496, 577, 768, 798
BstMWI GCNNNNNNNGC 2 cut(s) 40, 230
BstNI CCWGG 1 cut(s) 195
BstSCI CCNGG 2 cut(s) 193, 580
BstSFI CTRYAG 1 cut(s) 811
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 668
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 2 cut(s) 400, 577
BstYI RGATCY 2 cut(s) 400, 577
Bsu36I CCTNAGG 1 cut(s) 711
BsuRI GGCC 1 cut(s) 626
BtsCI GGATG 3 cut(s) 124, 244, 277
BtsIMutI CAGTG 4 cut(s) 87, 169, 265, 679
Cac8I GCNNGC 1 cut(s) 545
CfoI GCGC 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 26
Csp6I GTAC 1 cut(s) 410
CviAII CATG 5 cut(s) 50, 207, 256, 310, 701
CviJI RGCY 9 cut(s) 110, 188, 224, 233, 372, 547, 626, 659, 722
CviKI_1 RGCY 9 cut(s) 110, 188, 224, 233, 372, 547, 626, 659, 722
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 5 cut(s) 35, 134, 389, 570, 711
DpnI GATC 5 cut(s) 402, 498, 579, 770, 800
DpnII GATC 5 cut(s) 400, 496, 577, 768, 798
EaeI YGGCCR 1 cut(s) 624
Eam1104I CTCTTC 3 cut(s) 283, 454, 666
EarI CTCTTC 3 cut(s) 283, 454, 666
Eco130I CCWWGG 1 cut(s) 504
Eco31I GGTCTC 2 cut(s) 644, 671
Eco47I GGWCC 1 cut(s) 26
Eco47III AGCGCT 1 cut(s) 394
Eco81I CCTNAGG 1 cut(s) 711
EcoRI GAATTC 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 504
EcoT22I ATGCAT 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 2 cut(s) 444, 610
FaeI CATG 5 cut(s) 53, 210, 259, 313, 704
FaiI YATR 8 cut(s) 51, 150, 208, 257, 311, 359, 615, 702
FaqI GGGAC 1 cut(s) 448
FatI CATG 5 cut(s) 49, 206, 255, 309, 700
Fnu4HI GCNGC 2 cut(s) 657, 814
FokI GGATG 3 cut(s) 131, 251, 284
Fsp4HI GCNGC 2 cut(s) 657, 814
FspBI CTAG 2 cut(s) 234, 723
GlaI GCGC 1 cut(s) 394
GluI GCNGC 2 cut(s) 657, 814
GsuI CTGGAG 1 cut(s) 216
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 1 cut(s) 626
HapII CCGG 2 cut(s) 480, 581
HhaI GCGC 1 cut(s) 395
Hin1I GRCGYC 2 cut(s) 437, 603
Hin1II CATG 5 cut(s) 53, 210, 259, 313, 704
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HindIII AAGCTT 2 cut(s) 186, 370
HinfI GANTC 3 cut(s) 153, 421, 807
HpaII CCGG 2 cut(s) 480, 581
HphI GGTGA 3 cut(s) 330, 469, 766
Hpy188I TCNGA 4 cut(s) 38, 214, 652, 773
Hpy188III TCNNGA 3 cut(s) 592, 665, 730
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 5 cut(s) 24, 376, 463, 627, 727
HpyCH4III ACNGT 3 cut(s) 77, 164, 674
HpyCH4IV ACGT 3 cut(s) 437, 603, 690
HpyCH4V TGCA 8 cut(s) 43, 122, 377, 524, 543, 656, 791, 813
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 230
HpyF3I CTNAG 5 cut(s) 35, 134, 389, 570, 711
HpySE526I ACGT 3 cut(s) 437, 603, 690
Hsp92I GRCGYC 2 cut(s) 437, 603
Hsp92II CATG 5 cut(s) 53, 210, 259, 313, 704
HspAI GCGC 1 cut(s) 393
Kzo9I GATC 5 cut(s) 400, 496, 577, 768, 798
LguI GCTCTTC 2 cut(s) 454, 666
Lsp1109I GCAGC 1 cut(s) 668
LweI GCATC 3 cut(s) 109, 307, 643
MaeI CTAG 2 cut(s) 234, 723
MaeII ACGT 3 cut(s) 437, 603, 690
MaeIII GTNAC 2 cut(s) 422, 691
MalI GATC 5 cut(s) 402, 498, 579, 770, 800
MboI GATC 5 cut(s) 400, 496, 577, 768, 798
MboII GAAGA 6 cut(s) 87, 131, 300, 441, 544, 653
MflI RGATCY 2 cut(s) 400, 577
MlsI TGGCCA 1 cut(s) 626
MluCI AATT 2 cut(s) 91, 823
MluNI TGGCCA 1 cut(s) 626
MlyI GAGTC 3 cut(s) 162, 430, 816
MnlI CCTC 4 cut(s) 135, 137, 254, 351
Mox20I TGGCCA 1 cut(s) 626
Mph1103I ATGCAT 1 cut(s) 45
MscI TGGCCA 1 cut(s) 626
MseI TTAA 3 cut(s) 168, 822, 826
MslI CAYNNNNRTG 2 cut(s) 48, 270
Msp20I TGGCCA 1 cut(s) 626
MspI CCGG 2 cut(s) 480, 581
MspR9I CCNGG 2 cut(s) 195, 582
Mva1269I GAATGC 2 cut(s) 45, 793
MvaI CCWGG 1 cut(s) 195
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 2 cut(s) 40, 230
NciI CCSGG 1 cut(s) 582
NdeII GATC 5 cut(s) 400, 496, 577, 768, 798
NlaIII CATG 5 cut(s) 53, 210, 259, 313, 704
NlaIV GGNNCC 1 cut(s) 579
NmuCI GTSAC 1 cut(s) 422
NsiI ATGCAT 1 cut(s) 45
PacI TTAATTAA 1 cut(s) 826
PciSI GCTCTTC 2 cut(s) 454, 666
PctI GAATGC 2 cut(s) 45, 793
PfoI TCCNGGA 1 cut(s) 193
PkrI GCNGC 2 cut(s) 658, 815
PleI GAGTC 3 cut(s) 161, 429, 815
PpsI GAGTC 3 cut(s) 161, 429, 815
PshBI ATTAAT 1 cut(s) 822
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 1 cut(s) 579
PspPI GGNCC 1 cut(s) 26
PstI CTGCAG 1 cut(s) 815
PsuI RGATCY 2 cut(s) 400, 577
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
RseI CAYNNNNRTG 2 cut(s) 48, 270
SapI GCTCTTC 2 cut(s) 454, 666
SaqAI TTAA 3 cut(s) 168, 822, 826
SatI GCNGC 2 cut(s) 657, 814
Sau3AI GATC 5 cut(s) 400, 496, 577, 768, 798
Sau96I GGNCC 1 cut(s) 26
ScaI AGTACT 1 cut(s) 411
SchI GAGTC 3 cut(s) 162, 430, 816
ScrFI CCNGG 2 cut(s) 195, 582
SfaNI GCATC 3 cut(s) 109, 307, 643
SfcI CTRYAG 1 cut(s) 811
SinI GGWCC 1 cut(s) 26
SmiMI CAYNNNNRTG 2 cut(s) 48, 270
Sse9I AATT 2 cut(s) 91, 823
SsiI CCGC 2 cut(s) 5, 251
SspMI CTAG 2 cut(s) 234, 723
StyD4I CCNGG 2 cut(s) 193, 580
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 3 cut(s) 77, 164, 674
TaiI ACGT 3 cut(s) 440, 606, 693
TaqI TCGA 1 cut(s) 142
TasI AATT 2 cut(s) 91, 823
TatI WGTACW 1 cut(s) 409
Tru1I TTAA 3 cut(s) 168, 822, 826
Tru9I TTAA 3 cut(s) 168, 822, 826
TscAI CASTG 4 cut(s) 87, 169, 272, 679
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 2 cut(s) 656, 813
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 2 cut(s) 195, 551
TspGWI ACGGA 1 cut(s) 18
TspRI CASTG 4 cut(s) 87, 169, 272, 679
VpaK11BI GGWCC 1 cut(s) 26
VspI ATTAAT 1 cut(s) 822
XapI RAATTY 1 cut(s) 91
XspI CTAG 2 cut(s) 234, 723
ZraI GACGTC 2 cut(s) 438, 604
ZrmI AGTACT 1 cut(s) 411
Zsp2I ATGCAT 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.