Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0017309

Physical Location & Seq

Genomic Coordinates Forward (+)

12959613 .. 12963998

Gene Structure 4386 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG132200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGGCGTCAAGGGTCATATTTCTACCTCTCGACCTTGCTGTAAAGATACAACCCCTGTTGACTAAAATCTCAAAATTTGGGGGTAAGCTGGCTTCAGCTTCTTCTGTTCCTGAAGTGCAACTGTCTCAATATGTTCCTACACTTGAAAAGCTTGGTACTTTGAGGTTGCTCCAGCAGGTGTCTCGGGTGTATCAGTCAATGAAGATTGAACATTTATCTCGGATGATCCCATTTTATGACTTTTCTGTTGTAGAGAAGATATATGTGGATGCTGTTAAACATAACTTCATAGCTATGAAAGTCGACCATATGAAGGGTGTTATGATGTTTGGTAACTTGGCAAAAGCTAATGAGGGAGAGGGTCCAGAAATTCTAAGAAGTGCGCATGAAGGCGGTTCAAGTGGTCATGCCGAGTCATTAGCTGCAACACTAGCTGAACATCAGCAGCACTTGGTTAGCTTTCAGGTTCTCATTAATCAACTGAAGGAAGTTGCTCCAGCAATACAAAAGTCAATATCAAAATGTACAGATAAAGTAGATAGCATTTCCTCTAATCTGCCTCCAATGACTAAGCATCCTGGCCAGATGGAGAAGGTTACTTCAGTCTTTGGTTGTGAGCAAGCGGCTACAAAATTTGCTAAAGGTTATATTGAAAAGATCCATGTTCGTAGTTTGTGGGTGCTTTGTCGATGTATGTCTCTAGATGAATGTGAGCTGCCGATTGGACAAGATAGCAGCTTTATGTCCTTGCTAAAAAGGGGTACTGAAGAAAGCAATATACGTGGTGAAGCGTATATTGGCAGTAGCACACTGGAATCAATAAGACTTCTCGTCACTATTATTGGTACCACCCTGACTTCTGGTTTGATCAAGATGATGTTATGCCTAACTTTGGAGAACATTGAGTGGAGGATGCGAAAAATGAGCAATGCTGAGGCTTTACTTAGGTTTCTGAATGAGTCTTTTGAGTGGGAAGAAAAATCAGATGAGGGAGTGGTTTACAATCGAATGATTTGGTCTCTAGAACAGGTGCATAATGAGATGGGTTTGCTTCCAACTTTAGGGCCAAGGGAACTGGGAACCATTGCACAAAGTGATGGGCGTAAAATAAATCTGGATGACATAAATCAACGGCATGAATTTATCGATAGATTTCTTGCTGATACAGTCTCCATTGCTACAAGACTTGAGCTTCTCTTAACCTTCCCCTTGCTGCCTAATTGGCAGAGTTTTTTACATGCAACAAATGGTAATTCTTTGGATTGGCTTGAAGGGAAGATTAAAGTAATGTGTGCTCAAGTAGAAGTCATTAACTCTAAAGCAGATGCATTTCTTGGAAAATATGAGGGACAAAGCATTGAAATTCTCAACTTCCAGAGGAAAATGACAGCGCCCATTGAGAATATGTGGCAAAATTTGGGAGCTTTACTCTGGACTTCTAGAAATATGATGCGTGGAGATGGTCAAGAATATGAGGATGCAAAAAATGAACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001732 GO:0002181 GO:0002183 GO:0002188 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003743 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005852 GO:0005886 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016020 GO:0016043 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0043614 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0065003 GO:0071540 GO:0071541 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

PCI

Protein Analysis

498

Amino Acids

56.03

Weight (kDa)

6.11

Isoelectric Point (pI)

40.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCI	PF01399	40 - 110	1e-06	PCI domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0017309)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g24700 FvH4_5g24710
rosa_chinensis	RchiOBHm_Chr5g0018131
rosa_roxburghii	Rroxscaffold_1G00059670
rosa_samantha	Rh5AG132000 Rh5AG133400 Rh5CG142500 Rh5CG142600 Rh5CG142700 Rh5CG144100 Rh5DG132200
rosa_wichuraiana	Rw5G011490 Rw5G011630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	166
Acc16I	TGCGCA	1 cut(s)	384
Acc36I	ACCTGC	1 cut(s)	166
Acc65I	GGTACC	1 cut(s)	845
AccB1I	GGYRCC	1 cut(s)	845
AccI	GTMKAC	1 cut(s)	303
AciI	CCGC	2 cut(s)	393, 623
AclWI	GGATC	2 cut(s)	220, 652
AcoI	YGGCCR	1 cut(s)	580
AcsI	RAATTY	6 cut(s)	74, 369, 632, 1139, 1362, 1414
AcuI	CTGAAG	5 cut(s)	78, 132, 503, 585, 786
AcyI	GRCGYC	1 cut(s)	5
AdeI	CACNNNGTG	1 cut(s)	1094
AfaI	GTAC	4 cut(s)	157, 526, 763, 847
AfiI	CCNNNNNNNGG	3 cut(s)	313, 892, 1061
AgsI	TTSAA	6 cut(s)	146, 209, 399, 653, 1271, 1361
AhdI	GACNNNNNGTC	1 cut(s)	830
AjnI	CCWGG	1 cut(s)	577
AjuI	GAANNNNNNNTTGG	2 cut(s)	780, 812
Alw21I	GWGCWC	1 cut(s)	1297
Alw26I	GTCTC	5 cut(s)	129, 186, 702, 1023, 1174
AlwI	GGATC	2 cut(s)	220, 652
Ama87I	CYCGRG	1 cut(s)	183
AoxI	GGCC	2 cut(s)	580, 1064
ApeKI	GCWGC	5 cut(s)	422, 445, 715, 735, 1213
ApoI	RAATTY	6 cut(s)	74, 369, 632, 1139, 1362, 1414
ArsI	GACNNNNNNTTYG	2 cut(s)	847, 879
AseI	ATTAAT	1 cut(s)	474
Asp718I	GGTACC	1 cut(s)	845
AspLEI	GCGC	2 cut(s)	385, 1393
AspS9I	GGNCC	2 cut(s)	362, 1064
AsuHPI	GGTGA	1 cut(s)	797
AvaI	CYCGRG	1 cut(s)	183
AvaII	GGWCC	1 cut(s)	362
BalI	TGGCCA	1 cut(s)	582
BanI	GGYRCC	1 cut(s)	845
BarI	GAAGNNNNNNTAC	2 cut(s)	76, 108
Bbv12I	GWGCWC	1 cut(s)	1297
BbvCI	CCTCAGC	1 cut(s)	933
BbvI	GCAGC	5 cut(s)	409, 457, 702, 747, 1200
BccI	CCATC	4 cut(s)	580, 1036, 1091, 1454
BceAI	ACGGC	1 cut(s)	1148
BcgI	CGANNNNNNTGC	2 cut(s)	164, 198
BciT130I	CCWGG	1 cut(s)	579
BclI	TGATCA	1 cut(s)	867
BcoDI	GTCTC	5 cut(s)	129, 186, 702, 1023, 1174
BfaI	CTAG	4 cut(s)	431, 701, 1022, 1440
BfoI	RGCGCY	1 cut(s)	1394
BfuAI	ACCTGC	1 cut(s)	166
BglI	GCCNNNNNGGC	1 cut(s)	1222
BisI	GCNGC	6 cut(s)	423, 446, 624, 716, 736, 1214
BlsI	GCNGC	6 cut(s)	424, 447, 625, 717, 737, 1215
Bme1390I	CCNGG	1 cut(s)	579
Bme18I	GGWCC	1 cut(s)	362
BmeRI	GACNNNNNGTC	1 cut(s)	830
BmeT110I	CYCGRG	1 cut(s)	183
BmgT120I	GGNCC	2 cut(s)	362, 1064
BmiI	GGNNCC	3 cut(s)	363, 847, 1081
BmrFI	CCNGG	1 cut(s)	579
BmrI	ACTGGG	1 cut(s)	1085
BmsI	GCATC	6 cut(s)	259, 583, 903, 1315, 1440, 1468
BmuI	ACTGGG	1 cut(s)	1085
BplI	GAGNNNNNCTC	2 cut(s)	1413, 1445
BpmI	CTGGAG	2 cut(s)	155, 480
Bpu10I	CCTNAGC	1 cut(s)	933
BpuEI	CTTGAG	2 cut(s)	1208, 1281
Bsa29I	ATCGAT	1 cut(s)	1146
BsaAI	YACGTR	1 cut(s)	782
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	1023
BsaJI	CCNNGG	1 cut(s)	1067
Bsc4I	CCNNNNNNNGG	3 cut(s)	313, 892, 1061
Bse1I	ACTGG	2 cut(s)	816, 1080
Bse3DI	GCAATG	3 cut(s)	934, 1083, 1173
BseBI	CCWGG	1 cut(s)	579
BseCI	ATCGAT	1 cut(s)	1146
BseDI	CCNNGG	1 cut(s)	1067
BseGI	GGATG	6 cut(s)	228, 274, 574, 918, 1123, 1483
BseLI	CCNNNNNNNGG	3 cut(s)	313, 892, 1061
BseMI	GCAATG	3 cut(s)	934, 1083, 1173
BseMII	CTCAG	1 cut(s)	924
BseNI	ACTGG	2 cut(s)	816, 1080
BseXI	GCAGC	5 cut(s)	409, 457, 702, 747, 1200
BshFI	GGCC	2 cut(s)	582, 1066
BshNI	GGYRCC	1 cut(s)	845
BshVI	ATCGAT	1 cut(s)	1146
BsiHKAI	GWGCWC	1 cut(s)	1297
BsiHKCI	CYCGRG	1 cut(s)	183
BslFI	GGGAC	1 cut(s)	1362
BslI	CCNNNNNNNGG	3 cut(s)	313, 892, 1061
BsmAI	GTCTC	5 cut(s)	129, 186, 702, 1023, 1174
BsmFI	GGGAC	1 cut(s)	1362
BsnI	GGCC	2 cut(s)	582, 1066
Bso31I	GGTCTC	1 cut(s)	1023
BsoBI	CYCGRG	1 cut(s)	183
Bsp1286I	GDGCHC	1 cut(s)	1297
Bsp1407I	TGTACA	1 cut(s)	524
Bsp143I	GATC	3 cut(s)	225, 657, 867
BspACI	CCGC	2 cut(s)	393, 623
BspANI	GGCC	2 cut(s)	582, 1066
BspCNI	CTCAG	1 cut(s)	925
BspDI	ATCGAT	1 cut(s)	1146
BspLI	GGNNCC	3 cut(s)	363, 847, 1081
BspMI	ACCTGC	1 cut(s)	166
BspPI	GGATC	2 cut(s)	220, 652
BspT107I	GGYRCC	1 cut(s)	845
BspTNI	GGTCTC	1 cut(s)	1023
BsrDI	GCAATG	3 cut(s)	934, 1083, 1173
BsrGI	TGTACA	1 cut(s)	524
BsrI	ACTGG	2 cut(s)	816, 1080
BssECI	CCNNGG	1 cut(s)	1067
BssMI	GATC	3 cut(s)	225, 657, 867
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	1 cut(s)	1067
Bst2UI	CCWGG	1 cut(s)	579
Bst4CI	ACNGT	2 cut(s)	123, 1168
BstACI	GRCGYC	1 cut(s)	5
BstAUI	TGTACA	1 cut(s)	524
BstBAI	YACGTR	1 cut(s)	782
BstC8I	GCNNGC	2 cut(s)	90, 621
BstDEI	CTNAG	4 cut(s)	374, 570, 933, 944
BstF5I	GGATG	6 cut(s)	228, 274, 574, 918, 1123, 1483
BstH2I	RGCGCY	1 cut(s)	1394
BstHHI	GCGC	2 cut(s)	385, 1393
BstKTI	GATC	3 cut(s)	228, 660, 870
BstMAI	GTCTC	5 cut(s)	129, 186, 702, 1023, 1174
BstMBI	GATC	3 cut(s)	225, 657, 867
BstMWI	GCNNNNNNNGC	2 cut(s)	431, 1222
BstNI	CCWGG	1 cut(s)	579
BstNSI	RCATGY	1 cut(s)	1241
BstSCI	CCNGG	1 cut(s)	577
BstV1I	GCAGC	5 cut(s)	409, 457, 702, 747, 1200
BstX2I	RGATCY	1 cut(s)	657
BstYI	RGATCY	1 cut(s)	657
Bsu15I	ATCGAT	1 cut(s)	1146
BsuRI	GGCC	2 cut(s)	582, 1066
BsuTUI	ATCGAT	1 cut(s)	1146
BtsCI	GGATG	6 cut(s)	228, 274, 574, 918, 1123, 1483
BtsIMutI	CAGTG	1 cut(s)	809
BveI	ACCTGC	1 cut(s)	166
Cac8I	GCNNGC	2 cut(s)	90, 621
CfoI	GCGC	2 cut(s)	385, 1393
Cfr13I	GGNCC	2 cut(s)	362, 1064
ClaI	ATCGAT	1 cut(s)	1146
Csp6I	GTAC	4 cut(s)	156, 525, 762, 846
CspCI	CAANNNNNGTGG	2 cut(s)	763, 798
CviAII	CATG	5 cut(s)	386, 407, 662, 1136, 1238
CviQI	GTAC	4 cut(s)	156, 525, 762, 846
DdeI	CTNAG	4 cut(s)	374, 570, 933, 944
DpnI	GATC	3 cut(s)	227, 659, 869
DpnII	GATC	3 cut(s)	225, 657, 867
DraIII	CACNNNGTG	1 cut(s)	1094
DriI	GACNNNNNGTC	1 cut(s)	830
EaeI	YGGCCR	1 cut(s)	580
Eam1105I	GACNNNNNGTC	1 cut(s)	830
Eco130I	CCWWGG	1 cut(s)	1067
Eco31I	GGTCTC	1 cut(s)	1023
Eco47I	GGWCC	1 cut(s)	362
Eco57I	CTGAAG	5 cut(s)	78, 132, 503, 585, 786
Eco88I	CYCGRG	1 cut(s)	183
EcoRII	CCWGG	1 cut(s)	577
EcoT14I	CCWWGG	1 cut(s)	1067
EcoT22I	ATGCAT	1 cut(s)	1330
ErhI	CCWWGG	1 cut(s)	1067
FaeI	CATG	5 cut(s)	389, 410, 665, 1139, 1241
FaqI	GGGAC	1 cut(s)	1362
FatI	CATG	5 cut(s)	385, 406, 661, 1135, 1237
FauNDI	CATATG	1 cut(s)	309
FbaI	TGATCA	1 cut(s)	867
FblI	GTMKAC	1 cut(s)	303
Fnu4HI	GCNGC	6 cut(s)	423, 446, 624, 716, 736, 1214
FokI	GGATG	6 cut(s)	235, 281, 561, 925, 1130, 1490
Fsp4HI	GCNGC	6 cut(s)	423, 446, 624, 716, 736, 1214
FspAI	RTGCGCAY	1 cut(s)	384
FspBI	CTAG	4 cut(s)	431, 701, 1022, 1440
FspI	TGCGCA	1 cut(s)	384
GlaI	GCGC	2 cut(s)	384, 1392
GluI	GCNGC	6 cut(s)	423, 446, 624, 716, 736, 1214
GsuI	CTGGAG	2 cut(s)	155, 480
HaeII	RGCGCY	1 cut(s)	1394
HaeIII	GGCC	2 cut(s)	582, 1066
HhaI	GCGC	2 cut(s)	385, 1393
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	5 cut(s)	389, 410, 665, 1139, 1241
Hin6I	GCGC	2 cut(s)	383, 1391
HinP1I	GCGC	2 cut(s)	383, 1391
HincII	GTYRAC	2 cut(s)	60, 304
HindII	GTYRAC	2 cut(s)	60, 304
HindIII	AAGCTT	1 cut(s)	149
HinfI	GANTC	3 cut(s)	413, 815, 959
HphI	GGTGA	1 cut(s)	797
Hpy166II	GTNNAC	3 cut(s)	60, 304, 1000
Hpy188I	TCNGA	3 cut(s)	222, 954, 985
Hpy8I	GTNNAC	3 cut(s)	60, 304, 1000
HpyAV	CCTTC	6 cut(s)	307, 383, 478, 586, 1213, 1265
HpyCH4III	ACNGT	2 cut(s)	123, 1168
HpyCH4IV	ACGT	1 cut(s)	781
HpyCH4V	TGCA	7 cut(s)	118, 425, 1033, 1088, 1241, 1328, 1481
HpyF10VI	GCNNNNNNNGC	2 cut(s)	431, 1222
HpyF3I	CTNAG	4 cut(s)	374, 570, 933, 944
HpySE526I	ACGT	1 cut(s)	781
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	5 cut(s)	389, 410, 665, 1139, 1241
HspAI	GCGC	2 cut(s)	383, 1391
KpnI	GGTACC	1 cut(s)	849
Ksp22I	TGATCA	1 cut(s)	867
Kzo9I	GATC	3 cut(s)	225, 657, 867
LmnI	GCTCC	3 cut(s)	174, 499, 1421
Lsp1109I	GCAGC	5 cut(s)	409, 457, 702, 747, 1200
LweI	GCATC	6 cut(s)	259, 583, 903, 1315, 1440, 1468
MaeI	CTAG	4 cut(s)	431, 701, 1022, 1440
MaeII	ACGT	1 cut(s)	781
MaeIII	GTNAC	3 cut(s)	332, 595, 832
MalI	GATC	3 cut(s)	227, 659, 869
MboI	GATC	3 cut(s)	225, 657, 867
MboII	GAAGA	6 cut(s)	93, 214, 268, 779, 986, 1288
MflI	RGATCY	1 cut(s)	657
MhlI	GDGCHC	1 cut(s)	1297
MlsI	TGGCCA	1 cut(s)	582
MluCI	AATT	8 cut(s)	74, 369, 632, 1139, 1219, 1252, 1362, 1414
MluNI	TGGCCA	1 cut(s)	582
MlyI	GAGTC	2 cut(s)	422, 968
MmeI	TCCRAC	1 cut(s)	1079
Mox20I	TGGCCA	1 cut(s)	582
Mph1103I	ATGCAT	1 cut(s)	1330
MscI	TGGCCA	1 cut(s)	582
MseI	TTAA	5 cut(s)	276, 474, 1199, 1281, 1311
MslI	CAYNNNNRTG	1 cut(s)	293
Msp20I	TGGCCA	1 cut(s)	582
MspR9I	CCNGG	1 cut(s)	579
MvaI	CCWGG	1 cut(s)	579
MwoI	GCNNNNNNNGC	2 cut(s)	431, 1222
NdeI	CATATG	1 cut(s)	309
NdeII	GATC	3 cut(s)	225, 657, 867
NlaIII	CATG	5 cut(s)	389, 410, 665, 1139, 1241
NlaIV	GGNNCC	3 cut(s)	363, 847, 1081
NmeAIII	GCCGAG	1 cut(s)	436
NmuCI	GTSAC	1 cut(s)	832
NsbI	TGCGCA	1 cut(s)	384
NsiI	ATGCAT	1 cut(s)	1330
NspI	RCATGY	1 cut(s)	1241
PaqCI	CACCTGC	1 cut(s)	166
PfeI	GAWTC	1 cut(s)	815
PkrI	GCNGC	6 cut(s)	424, 447, 625, 717, 737, 1215
PleI	GAGTC	2 cut(s)	421, 967
PpsI	GAGTC	2 cut(s)	421, 967
Ppu21I	YACGTR	1 cut(s)	782
PshBI	ATTAAT	1 cut(s)	474
Psp6I	CCWGG	1 cut(s)	577
PspGI	CCWGG	1 cut(s)	577
PspN4I	GGNNCC	3 cut(s)	363, 847, 1081
PspPI	GGNCC	2 cut(s)	362, 1064
PsuI	RGATCY	1 cut(s)	657
RsaI	GTAC	4 cut(s)	157, 526, 763, 847
RsaNI	GTAC	4 cut(s)	156, 525, 762, 846
RseI	CAYNNNNRTG	1 cut(s)	293
SalI	GTCGAC	1 cut(s)	302
SaqAI	TTAA	5 cut(s)	276, 474, 1199, 1281, 1311
SatI	GCNGC	6 cut(s)	423, 446, 624, 716, 736, 1214
Sau3AI	GATC	3 cut(s)	225, 657, 867
Sau96I	GGNCC	2 cut(s)	362, 1064
SchI	GAGTC	2 cut(s)	422, 968
ScrFI	CCNGG	1 cut(s)	579
SduI	GDGCHC	1 cut(s)	1297
SfaNI	GCATC	6 cut(s)	259, 583, 903, 1315, 1440, 1468
SinI	GGWCC	1 cut(s)	362
SmiMI	CAYNNNNRTG	1 cut(s)	293
SmlI	CTYRAG	2 cut(s)	1187, 1296
SmoI	CTYRAG	2 cut(s)	1187, 1296
Sse9I	AATT	8 cut(s)	74, 369, 632, 1139, 1219, 1252, 1362, 1414
SsiI	CCGC	2 cut(s)	393, 623
SspMI	CTAG	4 cut(s)	431, 701, 1022, 1440
StyD4I	CCNGG	1 cut(s)	577
StyI	CCWWGG	1 cut(s)	1067
TaaI	ACNGT	2 cut(s)	123, 1168
TaiI	ACGT	1 cut(s)	784
TaqI	TCGA	5 cut(s)	30, 303, 688, 1006, 1146
TasI	AATT	8 cut(s)	74, 369, 632, 1139, 1219, 1252, 1362, 1414
TatI	WGTACW	1 cut(s)	524
TauI	GCSGC	1 cut(s)	626
TfiI	GAWTC	1 cut(s)	815
Tru1I	TTAA	5 cut(s)	276, 474, 1199, 1281, 1311
Tru9I	TTAA	5 cut(s)	276, 474, 1199, 1281, 1311
TscAI	CASTG	1 cut(s)	816
TseFI	GTSAC	1 cut(s)	832
TseI	GCWGC	5 cut(s)	422, 445, 715, 735, 1213
Tsp45I	GTSAC	1 cut(s)	832
TspDTI	ATGAA	7 cut(s)	215, 277, 311, 326, 402, 720, 1152
TspRI	CASTG	1 cut(s)	816
VpaK11BI	GGWCC	1 cut(s)	362
VspI	ATTAAT	1 cut(s)	474
XapI	RAATTY	6 cut(s)	74, 369, 632, 1139, 1362, 1414
XbaI	TCTAGA	3 cut(s)	700, 1021, 1439
XceI	RCATGY	1 cut(s)	1241
XmiI	GTMKAC	1 cut(s)	303
XspI	CTAG	4 cut(s)	431, 701, 1022, 1440
Zsp2I	ATGCAT	1 cut(s)	1330

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.