Rh5DG468700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0017633
Physical Location & Seq
Reverse (-)
74328836 .. 74329766
931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG468700.1

Sequence Viewer

Length: 306 bp
ATGAAGAGCAAGAAAACCATCTGCTTCTTCTGCATCCCTTCCTGGAAACAGAACGAAGAGAATGATCAAGAGAAGCACGACCACGAATCCCATTCCAGCTCTCGTGGTCGGCGCGGCAAGAGAAGAGGAGGAAACGTCGTCTCCACATCTCATAGTGATGATCATGCTGAAGGCAACACCGGTACAAGTACTACTACCGCCTCAAGCAATGATGTTGGCGCCGCGGCCGTTGTTATGGTGAGTACGGCCAACTTTTCTTCCGCTGACGGTGGTAACGCTGATGGTGGCGGCGATGGCCAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

10.56

Weight (kDa)

6.17

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017633)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37040 FvH4_5g15961
prunus_persica Prupe.6G068300_v2.0.a1
pyrus_communis pycom11g07830
rosa_chinensis RchiOBHm_Chr5g0066451
rosa_laevigata RLG00000035867
rosa_roxburghii Rroxscaffold_1G00014420 Rroxscaffold_1G00014430
rosa_samantha Rh5AG437400 Rh5BG453600 Rh5CG475900 Rh5DG468700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 218
AccII CGCG 2 cut(s) 114, 224
AciI CCGC 6 cut(s) 114, 198, 222, 224, 261, 288
AcoI YGGCCR 3 cut(s) 225, 246, 295
AcuI CTGAAG 1 cut(s) 189
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 3 cut(s) 184, 190, 244
AgeI ACCGGT 1 cut(s) 179
AjnI CCWGG 1 cut(s) 41
AluBI AGCT 1 cut(s) 99
AluI AGCT 1 cut(s) 99
Alw26I GTCTC 1 cut(s) 145
AoxI GGCC 3 cut(s) 225, 246, 295
AsiGI ACCGGT 1 cut(s) 179
AspLEI GCGC 2 cut(s) 114, 221
AsuHPI GGTGA 1 cut(s) 250
BalI TGGCCA 1 cut(s) 297
BanI GGYRCC 1 cut(s) 218
BauI CACGAG 1 cut(s) 102
BccI CCATC 3 cut(s) 26, 275, 287
BceAI ACGGC 2 cut(s) 212, 261
BciT130I CCWGG 1 cut(s) 43
BclI TGATCA 2 cut(s) 64, 160
BcoDI GTCTC 1 cut(s) 145
BfoI RGCGCY 1 cut(s) 222
BisI GCNGC 4 cut(s) 115, 222, 225, 289
BlsI GCNGC 4 cut(s) 116, 223, 226, 290
BmcAI AGTACT 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 43
BmiI GGNNCC 1 cut(s) 220
BmrFI CCNGG 1 cut(s) 43
BmsI GCATC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 187
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 179
BsaXI ACNNNNNCTCC 4 cut(s) 120, 125, 150, 155
Bse118I RCCGGY 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 214
BseBI CCWGG 1 cut(s) 43
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 214
BseRI GAGGAG 1 cut(s) 141
BseX3I CGGCCG 1 cut(s) 225
Bsh1236I CGCG 2 cut(s) 114, 224
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 3 cut(s) 227, 248, 297
BshNI GGYRCC 1 cut(s) 218
BshTI ACCGGT 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 228
BsiSI CCGG 1 cut(s) 180
BsmAI GTCTC 1 cut(s) 145
BsmBI CGTCTC 1 cut(s) 145
BsnI GGCC 3 cut(s) 227, 248, 297
Bsp143I GATC 2 cut(s) 64, 160
BspACI CCGC 6 cut(s) 114, 198, 222, 224, 261, 288
BspANI GGCC 3 cut(s) 227, 248, 297
BspFNI CGCG 2 cut(s) 114, 224
BspLI GGNNCC 1 cut(s) 220
BspT107I GGYRCC 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 179
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 2 cut(s) 64, 160
BssNI GRCGYC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 102
Bst2BI CACGAG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 2 cut(s) 51, 118
BstACI GRCGYC 1 cut(s) 219
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 33
BstFNI CGCG 2 cut(s) 114, 224
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 2 cut(s) 114, 221
BstKTI GATC 2 cut(s) 67, 163
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 2 cut(s) 64, 160
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 2 cut(s) 30, 294
BstNI CCWGG 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 41
BstUI CGCG 2 cut(s) 114, 224
BstZI CGGCCG 1 cut(s) 225
BsuRI GGCC 3 cut(s) 227, 248, 297
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 33
CfoI GCGC 2 cut(s) 114, 221
Cfr10I RCCGGY 1 cut(s) 179
Cfr42I CCGCGG 1 cut(s) 225
Csp6I GTAC 3 cut(s) 183, 189, 243
CspAI ACCGGT 1 cut(s) 179
CviAII CATG 1 cut(s) 164
CviJI RGCY 4 cut(s) 99, 227, 248, 297
CviKI_1 RGCY 4 cut(s) 99, 227, 248, 297
CviQI GTAC 3 cut(s) 183, 189, 243
DinI GGCGCC 1 cut(s) 220
DpnI GATC 2 cut(s) 66, 162
DpnII GATC 2 cut(s) 64, 160
EaeI YGGCCR 3 cut(s) 225, 246, 295
EagI CGGCCG 1 cut(s) 225
Eam1104I CTCTTC 2 cut(s) 51, 118
EarI CTCTTC 2 cut(s) 51, 118
EclXI CGGCCG 1 cut(s) 225
Eco52I CGGCCG 1 cut(s) 225
Eco57I CTGAAG 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 41
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
Esp3I CGTCTC 1 cut(s) 145
FaeI CATG 1 cut(s) 167
FaiI YATR 4 cut(s) 153, 165, 236, 304
FatI CATG 1 cut(s) 163
FbaI TGATCA 2 cut(s) 64, 160
Fnu4HI GCNGC 4 cut(s) 115, 222, 225, 289
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 4 cut(s) 115, 222, 225, 289
GlaI GCGC 2 cut(s) 113, 220
GluI GCNGC 4 cut(s) 115, 222, 225, 289
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 3 cut(s) 227, 248, 297
HapII CCGG 1 cut(s) 180
HhaI GCGC 2 cut(s) 114, 221
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 1 cut(s) 167
Hin6I GCGC 2 cut(s) 112, 219
HinP1I GCGC 2 cut(s) 112, 219
HinfI GANTC 1 cut(s) 86
HpaII CCGG 1 cut(s) 180
HphI GGTGA 1 cut(s) 250
Hpy188III TCNNGA 1 cut(s) 68
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 48, 164
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 1 cut(s) 33
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 294
HpySE526I ACGT 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 1 cut(s) 167
HspAI GCGC 2 cut(s) 112, 219
KasI GGCGCC 1 cut(s) 218
Ksp22I TGATCA 2 cut(s) 64, 160
KspI CCGCGG 1 cut(s) 225
Kzo9I GATC 2 cut(s) 64, 160
LpnPI CCDG 4 cut(s) 28, 55, 109, 193
LweI GCATC 1 cut(s) 42
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 272
MalI GATC 2 cut(s) 66, 162
MboI GATC 2 cut(s) 64, 160
MboII GAAGA 5 cut(s) 16, 19, 68, 135, 249
MlsI TGGCCA 1 cut(s) 297
MluCI AATT 1 cut(s) 299
MluNI TGGCCA 1 cut(s) 297
Mly113I GGCGCC 1 cut(s) 219
MnlI CCTC 3 cut(s) 119, 122, 211
Mox20I TGGCCA 1 cut(s) 297
MscI TGGCCA 1 cut(s) 297
MslI CAYNNNNRTG 1 cut(s) 156
Msp20I TGGCCA 1 cut(s) 297
MspA1I CMGCKG 2 cut(s) 224, 263
MspI CCGG 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 43
MvaI CCWGG 1 cut(s) 43
MvnI CGCG 2 cut(s) 114, 224
MwoI GCNNNNNNNGC 2 cut(s) 30, 294
NarI GGCGCC 1 cut(s) 219
NdeII GATC 2 cut(s) 64, 160
NlaIII CATG 1 cut(s) 167
NlaIV GGNNCC 1 cut(s) 220
PfeI GAWTC 1 cut(s) 86
PfoI TCCNGGA 1 cut(s) 41
PinAI ACCGGT 1 cut(s) 179
PkrI GCNGC 4 cut(s) 116, 223, 226, 290
PluTI GGCGCC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 41
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 220
RsaI GTAC 3 cut(s) 184, 190, 244
RsaNI GTAC 3 cut(s) 183, 189, 243
RseI CAYNNNNRTG 1 cut(s) 156
SacII CCGCGG 1 cut(s) 225
SatI GCNGC 4 cut(s) 115, 222, 225, 289
Sau3AI GATC 2 cut(s) 64, 160
ScaI AGTACT 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 43
SetI ASST 2 cut(s) 101, 138
SfaNI GCATC 1 cut(s) 42
SfoI GGCGCC 1 cut(s) 220
Sfr303I CCGCGG 1 cut(s) 225
SgrBI CCGCGG 1 cut(s) 225
SmiMI CAYNNNNRTG 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
Sse9I AATT 1 cut(s) 299
SsiI CCGC 6 cut(s) 114, 198, 222, 224, 261, 288
SspDI GGCGCC 1 cut(s) 218
StyD4I CCNGG 1 cut(s) 41
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 1 cut(s) 138
TasI AATT 1 cut(s) 299
TatI WGTACW 1 cut(s) 188
TauI GCSGC 4 cut(s) 117, 224, 227, 291
TfiI GAWTC 1 cut(s) 86
TspDTI ATGAA 1 cut(s) 17
ZrmI AGTACT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.