Rh5DG507700

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0017775
Physical Location & Seq
Forward (+)
79281967 .. 79286344
4378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG507700.1

Sequence Viewer

Length: 618 bp
ATGAAGAATCAATTGGACGACGTCATTAAGGAGAAGGATGGGTTCATGGTTGAGATTGGGAATTCGTTATCTAATGTTCGAGGAATGGCCATGGAAGGTCAGCCACTGGCCTGCAAGACCTCCCTAATTGTGCTTGGAGGCATGGAGAAATTATCAGCCACTTGCATAGTAATTTATATGAGGTACTTATATACTGTGCTTGAAGAGTTAGACATGGTAGGCTCTGTTACTTTTGTTGATCTTGCTGTAATCTCTGCTCCATGGTGTGGGACAAGTTTTGTAAGATCCCGTAAGCTAGCAGACCGATTCAAAGGAGTACAAAATGATCAGATGTTCTTTATCCCTTACAACTCCGGTGCAATTAATATATTCAATCGGCAAAGGAATAAGCAAGGACGTAAAACTCCACTATGGAAAATCCTGATGGGTGCTCCAAAACAACCGACAAACAAAGAATGTGGCTATTACGTCATGCGCTTTATGAGGGACCTAATCCTTGAAGATATTCAAGGTGTACTAGCTAAGTGGGAAGGAACTATGAAGACCACATACCTTCAAGAAGAAATCGATGAAGTCCGTGATGAGGGGGCAGAATTCGTTAGCGACAAGTATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

205

Amino Acids

23.38

Weight (kDa)

6.39

Isoelectric Point (pI)

32.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AccB7I CCANNNNNTGG 1 cut(s) 266
AclWI GGATC 1 cut(s) 279
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 2 cut(s) 61, 593
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 3 cut(s) 185, 318, 516
AfiI CCNNNNNNNGG 2 cut(s) 266, 583
AgsI TTSAA 6 cut(s) 203, 310, 373, 500, 509, 557
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AluBI AGCT 2 cut(s) 295, 521
AluI AGCT 2 cut(s) 295, 521
Alw21I GWGCWC 1 cut(s) 433
AlwI GGATC 1 cut(s) 279
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 2 cut(s) 87, 108
ApoI RAATTY 2 cut(s) 61, 593
AseI ATTAAT 1 cut(s) 363
Asp700I GAANNNNTTC 1 cut(s) 504
AspLEI GCGC 1 cut(s) 477
AspS9I GGNCC 1 cut(s) 487
AsuNHI GCTAGC 1 cut(s) 295
AvaII GGWCC 1 cut(s) 487
BalI TGGCCA 1 cut(s) 89
BarI GAAGNNNNNNTAC 2 cut(s) 533, 565
BbsI GAAGAC 1 cut(s) 548
Bbv12I GWGCWC 1 cut(s) 433
BccI CCATC 2 cut(s) 32, 418
BclI TGATCA 1 cut(s) 325
BfaI CTAG 2 cut(s) 296, 518
Bme18I GGWCC 1 cut(s) 487
BmgT120I GGNCC 1 cut(s) 487
BmiI GGNNCC 1 cut(s) 488
BmtI GCTAGC 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 548
Bsa29I ATCGAT 1 cut(s) 567
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 2 cut(s) 90, 260
BsaWI WCCGGW 1 cut(s) 353
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 583
Bse1I ACTGG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 2 cut(s) 90, 260
BseGI GGATG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 266, 583
BseNI ACTGG 1 cut(s) 111
BshFI GGCC 2 cut(s) 89, 110
BshVI ATCGAT 1 cut(s) 567
BsiHKAI GWGCWC 1 cut(s) 433
BsiSI CCGG 1 cut(s) 354
BslFI GGGAC 2 cut(s) 283, 500
BslI CCNNNNNNNGG 2 cut(s) 266, 583
BsmFI GGGAC 2 cut(s) 283, 500
BsnI GGCC 2 cut(s) 89, 110
Bsp1286I GDGCHC 1 cut(s) 433
Bsp143I GATC 3 cut(s) 238, 284, 325
Bsp19I CCATGG 2 cut(s) 90, 260
BspANI GGCC 2 cut(s) 89, 110
BspDI ATCGAT 1 cut(s) 567
BspLI GGNNCC 1 cut(s) 488
BspOI GCTAGC 1 cut(s) 299
BspPI GGATC 1 cut(s) 279
BsrI ACTGG 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 90, 260
BssMI GATC 3 cut(s) 238, 284, 325
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 2 cut(s) 90, 260
Bst4CI ACNGT 1 cut(s) 196
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 21
BstC8I GCNNGC 2 cut(s) 112, 297
BstDEI CTNAG 1 cut(s) 522
BstDSI CCRYGG 2 cut(s) 90, 260
BstF5I GGATG 1 cut(s) 43
BstHHI GCGC 1 cut(s) 477
BstKTI GATC 3 cut(s) 241, 287, 328
BstMBI GATC 3 cut(s) 238, 284, 325
BstV2I GAAGAC 1 cut(s) 548
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
Bsu15I ATCGAT 1 cut(s) 567
BsuRI GGCC 2 cut(s) 89, 110
BsuTUI ATCGAT 1 cut(s) 567
BtgI CCRYGG 2 cut(s) 90, 260
BtsCI GGATG 1 cut(s) 43
BtsIMutI CAGTG 1 cut(s) 104
Cac8I GCNNGC 2 cut(s) 112, 297
CaiI CAGNNNCTG 1 cut(s) 106
CfoI GCGC 1 cut(s) 477
Cfr13I GGNCC 1 cut(s) 487
ClaI ATCGAT 1 cut(s) 567
Csp6I GTAC 3 cut(s) 184, 317, 515
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviAII CATG 6 cut(s) 46, 91, 142, 214, 261, 472
CviJI RGCY 8 cut(s) 89, 103, 110, 158, 222, 295, 462, 521
CviKI_1 RGCY 8 cut(s) 89, 103, 110, 158, 222, 295, 462, 521
CviQI GTAC 3 cut(s) 184, 317, 515
DdeI CTNAG 1 cut(s) 522
DpnI GATC 3 cut(s) 240, 286, 327
DpnII GATC 3 cut(s) 238, 284, 325
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 2 cut(s) 90, 260
Eco47I GGWCC 1 cut(s) 487
EcoO109I RGGNCCY 1 cut(s) 487
EcoRI GAATTC 2 cut(s) 61, 593
EcoT14I CCWWGG 2 cut(s) 90, 260
ErhI CCWWGG 2 cut(s) 90, 260
FaeI CATG 6 cut(s) 49, 94, 145, 217, 264, 475
FaqI GGGAC 2 cut(s) 283, 500
FatI CATG 6 cut(s) 45, 90, 141, 213, 260, 471
FbaI TGATCA 1 cut(s) 325
FokI GGATG 1 cut(s) 50
FspBI CTAG 2 cut(s) 296, 518
GlaI GCGC 1 cut(s) 476
HaeIII GGCC 2 cut(s) 89, 110
HapII CCGG 1 cut(s) 354
HhaI GCGC 1 cut(s) 477
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 6 cut(s) 49, 94, 145, 217, 264, 475
Hin6I GCGC 1 cut(s) 475
HinP1I GCGC 1 cut(s) 475
HinfI GANTC 2 cut(s) 7, 306
HpaII CCGG 1 cut(s) 354
Hpy166II GTNNAC 1 cut(s) 515
Hpy188I TCNGA 1 cut(s) 330
Hpy188III TCNNGA 2 cut(s) 421, 557
Hpy8I GTNNAC 1 cut(s) 515
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 4 cut(s) 28, 89, 524, 563
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4IV ACGT 3 cut(s) 21, 397, 468
HpyCH4V TGCA 3 cut(s) 114, 165, 359
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 3 cut(s) 21, 397, 468
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 6 cut(s) 49, 94, 145, 217, 264, 475
HspAI GCGC 1 cut(s) 475
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 3 cut(s) 238, 284, 325
LmnI GCTCC 2 cut(s) 262, 436
LpnPI CCDG 4 cut(s) 92, 124, 367, 434
MaeI CTAG 2 cut(s) 296, 518
MaeII ACGT 3 cut(s) 21, 397, 468
MaeIII GTNAC 1 cut(s) 226
MalI GATC 3 cut(s) 240, 286, 327
MboI GATC 3 cut(s) 238, 284, 325
MboII GAAGA 5 cut(s) 16, 215, 512, 553, 572
MfeI CAATTG 1 cut(s) 11
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 433
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 7 cut(s) 11, 61, 126, 149, 171, 360, 593
MluNI TGGCCA 1 cut(s) 89
MnlI CCTC 6 cut(s) 74, 130, 131, 174, 477, 577
Mox20I TGGCCA 1 cut(s) 89
MroXI GAANNNNTTC 1 cut(s) 504
MscI TGGCCA 1 cut(s) 89
MseI TTAA 2 cut(s) 27, 363
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 1 cut(s) 354
MunI CAATTG 1 cut(s) 11
NcoI CCATGG 2 cut(s) 90, 260
NdeII GATC 3 cut(s) 238, 284, 325
NheI GCTAGC 1 cut(s) 295
NlaIII CATG 6 cut(s) 49, 94, 145, 217, 264, 475
NlaIV GGNNCC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 504
PfeI GAWTC 2 cut(s) 7, 306
PflFI GACNNNGTC 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 266
PpuMI RGGWCCY 1 cut(s) 487
PshBI ATTAAT 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 487
PspN4I GGNNCC 1 cut(s) 488
PspPI GGNCC 1 cut(s) 487
PspPPI RGGWCCY 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 284
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 3 cut(s) 185, 318, 516
RsaNI GTAC 3 cut(s) 184, 317, 515
SaqAI TTAA 2 cut(s) 27, 363
Sau3AI GATC 3 cut(s) 238, 284, 325
Sau96I GGNCC 1 cut(s) 487
SduI GDGCHC 1 cut(s) 433
SinI GGWCC 1 cut(s) 487
Sse9I AATT 7 cut(s) 11, 61, 126, 149, 171, 360, 593
SspMI CTAG 2 cut(s) 296, 518
StyI CCWWGG 2 cut(s) 90, 260
TaaI ACNGT 1 cut(s) 196
TaiI ACGT 3 cut(s) 24, 400, 471
TaqI TCGA 2 cut(s) 79, 567
TaqII GACCGA 1 cut(s) 318
TasI AATT 7 cut(s) 11, 61, 126, 149, 171, 360, 593
TatI WGTACW 2 cut(s) 316, 514
TfiI GAWTC 2 cut(s) 7, 306
Tru1I TTAA 2 cut(s) 27, 363
Tru9I TTAA 2 cut(s) 27, 363
TscAI CASTG 1 cut(s) 111
TspDTI ATGAA 4 cut(s) 17, 34, 554, 585
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 1 cut(s) 111
Tth111I GACNNNGTC 1 cut(s) 20
Van91I CCANNNNNTGG 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 487
VspI ATTAAT 1 cut(s) 363
XapI RAATTY 2 cut(s) 61, 593
XmnI GAANNNNTTC 1 cut(s) 504
XspI CTAG 2 cut(s) 296, 518
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.