Rh6AG516600

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0011650
Physical Location & Seq
Forward (+)
68627661 .. 68630260
2600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG516600.1

Sequence Viewer

Length: 789 bp
ATGCCACCACCTGGAAGTGGTGGGTTCCCATTTGGTGGTGGCTGCGGCAGTGGATCAAAAGGATTCAATCCTAGAAATGCAGAGGATATCTTTGCAGAATTCTTTGGAAGTACTCCTTTTGGATTTGGGTCATCAGGCCCTGGTAGGTCTTCAAGATTTGGGTCCGATGGAGGAGGGATGTTTGGGGGATTTGGTGGAAGCAGCGAAAATGTTTTTCGATCATATAGTGAGAACGTTACACCAAAGAAACCACCAGCAGTGGAGAGCAAGTTGCCTTGCAGCCTCGAGGAGCTGTACACTGGTTCAACAAGGAAAATGAAAATCTCTAGGACTGTGGTTGATGCAAATGGACGTCAAGCGCCAGAAACAGAAATACTAACCATTGATGTGAAGCCAGGATGGAAAAAAGGAACCAAGATCACATTTCCAGATAAAGGCAATGAACAGCCCGGCCAGTTACCTGCAGACCTTGTTTTTGTGATTGATGAGAAGCCCCATGACGTTTACAAGAGGGACAGCAATGACCTCCTCATTACAAGAAAGGTGTCATTAGCTGAGGCATTGGGTGGAACTGCAGTGCATCTTAAAACGCTAGATGGGCGCGACCTATCAATCCCGGTGACTGAAATTGTAAGCCCTGGCTATGAGCTTGTTGTTGCTAGGGAGGGTATGCCAATAGCAAAAGAGCCTGGAAATAGGGGTGATTTGAAGATCAAATTCGAGGTCAGGTTCCCTACAACATTAACCCCGGAACAACGAGCTGGACTTAAACGCACCTTGAAAGGTTGA

Protein Analysis

262

Amino Acids

27.98

Weight (kDa)

8.97

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 87 - 245 8.4e-39 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011650)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25530 AT5G25530
fragaria_vesca FvH4_2g30150
malus_domestica MD02G1290100.v1.1 MD07G1036900.v1.1 MD08G1136700.v1.1
prunus_persica Prupe.1G466900_v2.0.a1 Prupe.1G466900_v2.0.a1
pyrus_communis pycom08g11550
rosa_chinensis RchiOBHm_Chr6g0312781
rosa_laevigata RLG00000010300
rosa_multiflora Rmu_co8483235.1_g000001
rosa_roxburghii Rroxscaffold_7G00156500
rosa_rugosa Rorug06G0399600
rosa_samantha Rh6AG516600 Rh6BG527900 Rh6CG532700 Rh6DG518900
rosa_wichuraiana Rw6G044870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 355
AbsI CCTCGAGG 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 469
AccB7I CCANNNNNTGG 2 cut(s) 11, 35
AccII CGCG 1 cut(s) 603
AciI CCGC 1 cut(s) 45
AclI AACGTT 1 cut(s) 234
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 2 cut(s) 98, 716
AcyI GRCGYC 1 cut(s) 352
AfaI GTAC 2 cut(s) 112, 296
AfiI CCNNNNNNNGG 4 cut(s) 11, 17, 35, 434
AgsI TTSAA 5 cut(s) 67, 153, 306, 709, 781
AjnI CCWGG 5 cut(s) 10, 139, 394, 637, 688
AluBI AGCT 4 cut(s) 292, 554, 649, 761
AluI AGCT 4 cut(s) 292, 554, 649, 761
AlwI GGATC 1 cut(s) 61
AlwNI CAGNNNCTG 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 2 cut(s) 136, 451
ApeKI GCWGC 3 cut(s) 42, 201, 279
ApoI RAATTY 2 cut(s) 98, 716
ArsI GACNNNNNNTTYG 4 cut(s) 458, 490, 708, 740
AspLEI GCGC 2 cut(s) 361, 603
AspS9I GGNCC 2 cut(s) 137, 162
AsuC2I CCSGG 3 cut(s) 450, 617, 749
AsuHPI GGTGA 2 cut(s) 631, 713
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 141
BbvCI CCTCAGC 1 cut(s) 555
BbvI GCAGC 3 cut(s) 29, 213, 291
BccI CCATC 3 cut(s) 161, 393, 590
BciT130I CCWGG 5 cut(s) 12, 141, 396, 639, 690
BcnI CCSGG 3 cut(s) 450, 617, 749
BfaI CTAG 4 cut(s) 72, 327, 593, 660
BfmI CTRYAG 2 cut(s) 462, 573
BfoI RGCGCY 1 cut(s) 362
BfuAI ACCTGC 1 cut(s) 469
BisI GCNGC 4 cut(s) 43, 46, 202, 280
BlsI GCNGC 4 cut(s) 44, 47, 203, 281
BmcAI AGTACT 1 cut(s) 112
Bme1390I CCNGG 8 cut(s) 12, 141, 396, 450, 617, 639, 690, 749
Bme18I GGWCC 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 137, 162
BmiI GGNNCC 4 cut(s) 26, 163, 412, 731
BmrFI CCNGG 8 cut(s) 12, 141, 396, 450, 617, 639, 690, 749
BmsI GCATC 2 cut(s) 331, 589
BpiI GAAGAC 1 cut(s) 141
Bpu10I CCTNAGC 1 cut(s) 555
BpuMI CCSGG 3 cut(s) 450, 617, 749
BsaHI GRCGYC 1 cut(s) 352
BsaJI CCNNGG 3 cut(s) 139, 637, 747
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 4 cut(s) 11, 17, 35, 434
Bse1I ACTGG 2 cut(s) 304, 454
Bse3DI GCAATG 2 cut(s) 445, 526
BseBI CCWGG 5 cut(s) 12, 141, 396, 639, 690
BseDI CCNNGG 3 cut(s) 139, 637, 747
BseGI GGATG 2 cut(s) 183, 404
BseLI CCNNNNNNNGG 4 cut(s) 11, 17, 35, 434
BseMI GCAATG 2 cut(s) 445, 526
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 2 cut(s) 304, 454
BseRI GAGGAG 3 cut(s) 186, 302, 518
BseXI GCAGC 3 cut(s) 29, 213, 291
Bsh1236I CGCG 1 cut(s) 603
BshFI GGCC 2 cut(s) 138, 453
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 3 cut(s) 450, 617, 749
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 4 cut(s) 11, 17, 35, 434
BsmFI GGGAC 1 cut(s) 527
BsnI GGCC 2 cut(s) 138, 453
BsoBI CYCGRG 1 cut(s) 284
Bsp1407I TGTACA 1 cut(s) 294
Bsp143I GATC 4 cut(s) 53, 218, 417, 711
BspACI CCGC 1 cut(s) 45
BspANI GGCC 2 cut(s) 138, 453
BspCNI CTCAG 1 cut(s) 547
BspFNI CGCG 1 cut(s) 603
BspLI GGNNCC 4 cut(s) 26, 163, 412, 731
BspMAI CTGCAG 2 cut(s) 466, 577
BspMI ACCTGC 1 cut(s) 469
BspPI GGATC 1 cut(s) 61
BsrDI GCAATG 2 cut(s) 445, 526
BsrGI TGTACA 1 cut(s) 294
BsrI ACTGG 2 cut(s) 304, 454
BssECI CCNNGG 3 cut(s) 139, 637, 747
BssMI GATC 4 cut(s) 53, 218, 417, 711
BssNI GRCGYC 1 cut(s) 352
Bst2UI CCWGG 5 cut(s) 12, 141, 396, 639, 690
Bst4CI ACNGT 1 cut(s) 334
BstACI GRCGYC 1 cut(s) 352
BstAUI TGTACA 1 cut(s) 294
BstDEI CTNAG 1 cut(s) 555
BstF5I GGATG 2 cut(s) 183, 404
BstFNI CGCG 1 cut(s) 603
BstH2I RGCGCY 1 cut(s) 362
BstHHI GCGC 2 cut(s) 361, 603
BstKTI GATC 4 cut(s) 56, 221, 420, 714
BstMBI GATC 4 cut(s) 53, 218, 417, 711
BstMWI GCNNNNNNNGC 1 cut(s) 598
BstNI CCWGG 5 cut(s) 12, 141, 396, 639, 690
BstSCI CCNGG 8 cut(s) 10, 139, 394, 448, 615, 637, 688, 747
BstSFI CTRYAG 2 cut(s) 462, 573
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 3 cut(s) 29, 213, 291
BstV2I GAAGAC 1 cut(s) 141
BsuRI GGCC 2 cut(s) 138, 453
BtsCI GGATG 2 cut(s) 183, 404
BtsI GCAGTG 3 cut(s) 55, 264, 582
BtsIMutI CAGTG 4 cut(s) 55, 264, 297, 582
BveI ACCTGC 1 cut(s) 469
CaiI CAGNNNCTG 1 cut(s) 140
CfoI GCGC 2 cut(s) 361, 603
Cfr13I GGNCC 2 cut(s) 137, 162
Csp6I GTAC 2 cut(s) 111, 295
CviAII CATG 1 cut(s) 497
CviQI GTAC 2 cut(s) 111, 295
DdeI CTNAG 1 cut(s) 555
DpnI GATC 4 cut(s) 55, 220, 419, 713
DpnII GATC 4 cut(s) 53, 218, 417, 711
EaeI YGGCCR 1 cut(s) 451
Eco32I GATATC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 98
EcoRII CCWGG 5 cut(s) 10, 139, 394, 637, 688
EcoRV GATATC 1 cut(s) 88
FaeI CATG 1 cut(s) 500
FaiI YATR 5 cut(s) 223, 225, 498, 645, 671
FalI AAGNNNNNCTT 2 cut(s) 100, 132
FaqI GGGAC 1 cut(s) 527
FatI CATG 1 cut(s) 496
Fnu4HI GCNGC 4 cut(s) 43, 46, 202, 280
FokI GGATG 2 cut(s) 190, 411
Fsp4HI GCNGC 4 cut(s) 43, 46, 202, 280
FspBI CTAG 4 cut(s) 72, 327, 593, 660
GlaI GCGC 2 cut(s) 360, 602
GluI GCNGC 4 cut(s) 43, 46, 202, 280
HaeII RGCGCY 1 cut(s) 362
HaeIII GGCC 2 cut(s) 138, 453
HapII CCGG 3 cut(s) 450, 617, 749
HhaI GCGC 2 cut(s) 361, 603
Hin1I GRCGYC 1 cut(s) 352
Hin1II CATG 1 cut(s) 500
Hin6I GCGC 2 cut(s) 359, 601
HinP1I GCGC 2 cut(s) 359, 601
HinfI GANTC 1 cut(s) 63
HpaII CCGG 3 cut(s) 450, 617, 749
HphI GGTGA 2 cut(s) 631, 713
Hpy166II GTNNAC 2 cut(s) 297, 505
Hpy188I TCNGA 1 cut(s) 166
Hpy188III TCNNGA 2 cut(s) 153, 428
Hpy8I GTNNAC 2 cut(s) 297, 505
HpyCH4III ACNGT 1 cut(s) 334
HpyCH4IV ACGT 3 cut(s) 234, 352, 501
HpyCH4V TGCA 7 cut(s) 80, 95, 279, 344, 464, 575, 580
HpyF10VI GCNNNNNNNGC 1 cut(s) 598
HpyF3I CTNAG 1 cut(s) 555
HpySE526I ACGT 3 cut(s) 234, 352, 501
Hsp92I GRCGYC 1 cut(s) 352
Hsp92II CATG 1 cut(s) 500
HspAI GCGC 2 cut(s) 359, 601
Kzo9I GATC 4 cut(s) 53, 218, 417, 711
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 3 cut(s) 29, 213, 291
LweI GCATC 2 cut(s) 331, 589
MaeI CTAG 4 cut(s) 72, 327, 593, 660
MaeII ACGT 3 cut(s) 234, 352, 501
MaeIII GTNAC 3 cut(s) 235, 456, 619
MalI GATC 4 cut(s) 55, 220, 419, 713
MboI GATC 4 cut(s) 53, 218, 417, 711
MboII GAAGA 2 cut(s) 141, 721
MluCI AATT 3 cut(s) 98, 627, 716
MseI TTAA 3 cut(s) 585, 743, 768
MslI CAYNNNNRTG 1 cut(s) 386
MspI CCGG 3 cut(s) 450, 617, 749
MspR9I CCNGG 8 cut(s) 12, 141, 396, 450, 617, 639, 690, 749
MvaI CCWGG 5 cut(s) 12, 141, 396, 639, 690
MvnI CGCG 1 cut(s) 603
MwoI GCNNNNNNNGC 1 cut(s) 598
NciI CCSGG 3 cut(s) 450, 617, 749
NdeII GATC 4 cut(s) 53, 218, 417, 711
NlaIII CATG 1 cut(s) 500
NlaIV GGNNCC 4 cut(s) 26, 163, 412, 731
NmuCI GTSAC 1 cut(s) 619
PaeR7I CTCGAG 1 cut(s) 284
PfeI GAWTC 1 cut(s) 63
PflMI CCANNNNNTGG 2 cut(s) 11, 35
PkrI GCNGC 4 cut(s) 44, 47, 203, 281
Psp1406I AACGTT 1 cut(s) 234
Psp6I CCWGG 5 cut(s) 10, 139, 394, 637, 688
PspGI CCWGG 5 cut(s) 10, 139, 394, 637, 688
PspN4I GGNNCC 4 cut(s) 26, 163, 412, 731
PspPI GGNCC 2 cut(s) 137, 162
PspXI VCTCGAGB 1 cut(s) 284
PstI CTGCAG 2 cut(s) 466, 577
PstNI CAGNNNCTG 1 cut(s) 140
RsaI GTAC 2 cut(s) 112, 296
RsaNI GTAC 2 cut(s) 111, 295
RseI CAYNNNNRTG 1 cut(s) 386
SaqAI TTAA 3 cut(s) 585, 743, 768
SatI GCNGC 4 cut(s) 43, 46, 202, 280
Sau3AI GATC 4 cut(s) 53, 218, 417, 711
Sau96I GGNCC 2 cut(s) 137, 162
ScaI AGTACT 1 cut(s) 112
ScrFI CCNGG 8 cut(s) 12, 141, 396, 450, 617, 639, 690, 749
SfaNI GCATC 2 cut(s) 331, 589
SfcI CTRYAG 2 cut(s) 462, 573
Sfr274I CTCGAG 1 cut(s) 284
SinI GGWCC 1 cut(s) 162
SlaI CTCGAG 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 3 cut(s) 98, 627, 716
SsiI CCGC 1 cut(s) 45
SspMI CTAG 4 cut(s) 72, 327, 593, 660
StyD4I CCNGG 8 cut(s) 10, 139, 394, 448, 615, 637, 688, 747
TaaI ACNGT 1 cut(s) 334
TaiI ACGT 3 cut(s) 237, 355, 504
TaqI TCGA 3 cut(s) 217, 285, 720
TasI AATT 3 cut(s) 98, 627, 716
TatI WGTACW 2 cut(s) 110, 294
TauI GCSGC 1 cut(s) 48
TfiI GAWTC 1 cut(s) 63
Tru1I TTAA 3 cut(s) 585, 743, 768
Tru9I TTAA 3 cut(s) 585, 743, 768
TscAI CASTG 4 cut(s) 55, 264, 304, 582
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 3 cut(s) 42, 201, 279
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 2 cut(s) 332, 456
TspRI CASTG 4 cut(s) 55, 264, 304, 582
Van91I CCANNNNNTGG 2 cut(s) 11, 35
VpaK11BI GGWCC 1 cut(s) 162
XapI RAATTY 2 cut(s) 98, 716
XhoI CTCGAG 1 cut(s) 284
XspI CTAG 4 cut(s) 72, 327, 593, 660
ZraI GACGTC 1 cut(s) 353
ZrmI AGTACT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.