Rh6DG043000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002360
Physical Location & Seq
Forward (+)
4611103 .. 4621766
10664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG043000.1

Sequence Viewer

Length: 1749 bp
ATGTGGAATTTCTTTACTCAGAATTTGGAAGCTGAGAAGCAGCTTAAATTTTTCCAGGGCTGTGTAGCTGCAGCTTTTGCCGAACGGGATCAGTCCATAATGGAGGCTGAGACGGCTAGAGAGAAAGAGGAGCTCATGTCAAAGAAATTGAGTGAGATAGAGAAGAGGTTAGAAGAGCATACTGCAGATTGTTTTGAACAGAAGGAACTTAATGATAAATTGCAAACTGATTTAGCAATGCAAGAGGAGCAGAATGCAACTTTTAAGAAGGTCATCGATAAGTTTTATGAGATCAGGCAGGATTCTTCAGAGGGATTTGGGGATACAAGTTGGGACACCAAATGTGCATGCCTTTTGGATGATCCTGCAGAATGGTGGAGTTTCAATGACACTTCTACATCTAAATATATTAGTTCATTAGAAGAAGAGCTCGAGAGGGTGAGGATCTCTGCTGGTAATCTTCAAAACAGGCTACGAGTGGGTTTGGAAATTGAAAATCATTTAAAGAGGAGAGTTTCGGAATTGGAAAAGAAGAAAATCCGTTCAGATGGAATGATAAAAAAGGAGATGGCAGAATTACGTCATTACCATTCTCAGCATAAAGTTCACATCATGAGTTTACTTAATGAGGGAAGCAGTTGTATCAAATCAATAATTGATGCAGTGGAGAAAAAGTGTAGGCAAATTGATGCAAGCAGACTACAAAAGTTGGAGCCACCTATGGGAGATGTAAAGCAAGACGAATCTGAATGTCAAGATGTACATGAAATTACAGATGCTGATCCCCAAGTGGTATTTAAGGATGGTAACCCTAGCTTATCAGACATGGTGGCAGTTGGGGACGGTGATTCCTCTAGAGCCCGTGCTCAGGCACTGCAAGACAAGGTGTTCTGCAAAGATGGTGACCCTAGCTTATCAGATATGGTGGCAGTTGGGGAGGGTGATTCCTCTAAAGCCCTTGCACAGGCACTGGAAGACAAGGTTGCAACGTTATTGCTTTTATCACAGCAGGAAGAAAGACATTTACTGGACAGAAATGTGAATGCAGCTCTTCAAAGTAAAATAGATGAGCTTCAGAGAAACCTATTACAGGTTACCAATGAGAAGGTCCAAGCTCTAATGGAGATGGCACAATTAAAGCAGGAATATCATCAACTCAAAGAGAGAATGGGTCAGGAGACGAAAAGAGGCAATTCATCTGCTCAAGTTGGGGACAGGAGAGTAGTTACACATGAAAGAGATGGAAAACTAAAGAGCTTGCTGAAGAAAACGTACTTGAGCCGTTGGGTTGGAAATCTAGACCTTAGGGGCAATGAAGCTGAATCTGACCTAAATAGCATGGAGTTTGTGAGGATGAAGATTGAAAATGCAACCCTTAGGGAAACCATTGGAAGTATCGAACATCTGTCTACTACAGTTCATAGGCTTCGTCTATCGCTTTTAAAGGCCAAGGAGATGGTGACTTCTGGAGACTCGATGCTTACTAGCATGTTAGAAACTGTAGACAACATTATTAACGAGGCAAAACTAGTGAAGACCGCACTCGGAAGTTCCCTACCACTTAGTTGGTCAGCAGAAGCAGATGTGGAATTCAGTGGTTATAGTGTTAGCAGTGAGCCTGGTATCGTATATGGGGAGCCCAGCCATGAGAAGATAGATTCAGTTTATGCTGCAGGGTTTGAGATGGTGGAACTCCTGATATTAGCATCGCAGATATTAAAGGACAACACAAGCAAAAGTGGGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

582

Amino Acids

65.65

Weight (kDa)

5.14

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 1409, 1503
AciI CCGC 1 cut(s) 1539
AclI AACGTT 1 cut(s) 989
AclWI GGATC 4 cut(s) 96, 356, 452, 776
AcsI RAATTY 4 cut(s) 7, 22, 47, 1589
AcuI CTGAAG 3 cut(s) 291, 1058, 1283
AfaI GTAC 2 cut(s) 762, 1274
AfiI CCNNNNNNNGG 4 cut(s) 722, 868, 964, 1090
AgsI TTSAA 6 cut(s) 197, 385, 464, 494, 1055, 1364
AhlI ACTAGT 1 cut(s) 1528
AjnI CCWGG 2 cut(s) 54, 1618
Alw21I GWGCWC 3 cut(s) 135, 432, 868
Alw26I GTCTC 3 cut(s) 104, 1172, 1464
AlwI GGATC 4 cut(s) 96, 356, 452, 776
AlwNI CAGNNNCTG 3 cut(s) 779, 874, 970
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 1 cut(s) 1446
ApeKI GCWGC 5 cut(s) 40, 68, 71, 1046, 1670
ApoI RAATTY 4 cut(s) 7, 22, 47, 1589
AspS9I GGNCC 2 cut(s) 1108, 1743
AsuHPI GGTGA 5 cut(s) 451, 857, 914, 953, 1471
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 2 cut(s) 1108, 1743
AxyI CCTNAGG 2 cut(s) 1304, 1376
BanII GRGCYC 4 cut(s) 135, 432, 862, 1641
BarI GAAGNNNNNNTAC 2 cut(s) 1256, 1288
BbsI GAAGAC 2 cut(s) 981, 1541
Bbv12I GWGCWC 3 cut(s) 135, 432, 868
BbvI GCAGC 5 cut(s) 52, 55, 83, 1058, 1657
BccI CCATC 8 cut(s) 542, 562, 797, 893, 1120, 1235, 1450, 1678
BceAI ACGGC 2 cut(s) 129, 1266
BciT130I CCWGG 2 cut(s) 56, 1620
BciVI GTATCC 1 cut(s) 316
BcoDI GTCTC 3 cut(s) 104, 1172, 1464
BcuI ACTAGT 1 cut(s) 1528
BfaI CTAG 7 cut(s) 117, 813, 855, 909, 1298, 1485, 1529
BfmI CTRYAG 6 cut(s) 69, 183, 366, 1413, 1500, 1671
BfuI GTATCC 1 cut(s) 316
BisI GCNGC 5 cut(s) 41, 69, 72, 1047, 1671
BlsI GCNGC 5 cut(s) 42, 70, 73, 1048, 1672
Bme1390I CCNGG 2 cut(s) 56, 1620
Bme18I GGWCC 2 cut(s) 1108, 1743
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 1108, 1743
BmiI GGNNCC 3 cut(s) 714, 1638, 1744
BmrFI CCNGG 2 cut(s) 56, 1620
BmsI GCATC 5 cut(s) 649, 679, 766, 1467, 1715
BpiI GAAGAC 2 cut(s) 981, 1541
BpmI CTGGAG 1 cut(s) 1488
Bpu10I CCTNAGC 1 cut(s) 867
BpuEI CTTGAG 2 cut(s) 1188, 1297
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 780
BsaJI CCNNGG 2 cut(s) 55, 1449
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 659, 689
Bsc4I CCNNNNNNNGG 4 cut(s) 722, 868, 964, 1090
Bse1I ACTGG 2 cut(s) 975, 1032
Bse21I CCTNAGG 2 cut(s) 1304, 1376
Bse3DI GCAATG 2 cut(s) 243, 1318
Bse8I GATNNNNATC 1 cut(s) 780
BseBI CCWGG 2 cut(s) 56, 1620
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 2 cut(s) 55, 1449
BseGI GGATG 3 cut(s) 364, 808, 1359
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 4 cut(s) 722, 868, 964, 1090
BseMI GCAATG 2 cut(s) 243, 1318
BseMII CTCAG 5 cut(s) 24, 32, 99, 608, 881
BseNI ACTGG 2 cut(s) 975, 1032
BseRI GAGGAG 3 cut(s) 143, 260, 523
BseXI GCAGC 5 cut(s) 52, 55, 83, 1058, 1657
BseYI CCCAGC 1 cut(s) 1640
BshFI GGCC 1 cut(s) 1448
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 3 cut(s) 135, 432, 868
BsiHKCI CYCGRG 1 cut(s) 431
BslFI GGGAC 3 cut(s) 347, 854, 1226
BslI CCNNNNNNNGG 4 cut(s) 722, 868, 964, 1090
BsmAI GTCTC 3 cut(s) 104, 1172, 1464
BsmBI CGTCTC 2 cut(s) 104, 1172
BsmFI GGGAC 3 cut(s) 347, 854, 1226
BsmI GAATGC 2 cut(s) 259, 1048
BsnI GGCC 1 cut(s) 1448
BsoBI CYCGRG 1 cut(s) 431
Bsp1286I GDGCHC 5 cut(s) 135, 432, 862, 868, 1641
Bsp1407I TGTACA 1 cut(s) 760
Bsp143I GATC 5 cut(s) 88, 291, 361, 444, 781
BspACI CCGC 1 cut(s) 1539
BspANI GGCC 1 cut(s) 1448
BspCNI CTCAG 5 cut(s) 25, 31, 100, 607, 880
BspDI ATCGAT 1 cut(s) 276
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 3 cut(s) 714, 1638, 1744
BspMAI CTGCAG 4 cut(s) 73, 187, 370, 1675
BspPI GGATC 4 cut(s) 96, 356, 452, 776
BspQI GCTCTTC 3 cut(s) 168, 420, 1056
BsrDI GCAATG 2 cut(s) 243, 1318
BsrGI TGTACA 1 cut(s) 760
BsrI ACTGG 2 cut(s) 975, 1032
BssECI CCNNGG 2 cut(s) 55, 1449
BssMI GATC 5 cut(s) 88, 291, 361, 444, 781
BssT1I CCWWGG 1 cut(s) 1449
Bst2UI CCWGG 2 cut(s) 56, 1620
Bst4CI ACNGT 3 cut(s) 845, 1417, 1501
Bst6I CTCTTC 4 cut(s) 158, 168, 420, 1056
BstAPI GCANNNNNTGC 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 760
BstC8I GCNNGC 3 cut(s) 349, 694, 1259
BstDEI CTNAG 8 cut(s) 18, 33, 108, 594, 867, 1304, 1376, 1562
BstEII GGTNACC 3 cut(s) 806, 902, 1093
BstENI CCTNNNNNAGG 2 cut(s) 962, 1088
BstF5I GGATG 3 cut(s) 364, 808, 1359
BstKTI GATC 5 cut(s) 91, 294, 364, 447, 784
BstMAI GTCTC 3 cut(s) 104, 1172, 1464
BstMBI GATC 5 cut(s) 88, 291, 361, 444, 781
BstMWI GCNNNNNNNGC 3 cut(s) 77, 113, 247
BstNI CCWGG 2 cut(s) 56, 1620
BstNSI RCATGY 2 cut(s) 351, 1492
BstPI GGTNACC 3 cut(s) 806, 902, 1093
BstSCI CCNGG 2 cut(s) 54, 1618
BstSFI CTRYAG 6 cut(s) 69, 183, 366, 1413, 1500, 1671
BstV1I GCAGC 5 cut(s) 52, 55, 83, 1058, 1657
BstV2I GAAGAC 2 cut(s) 981, 1541
BstX2I RGATCY 1 cut(s) 444
BstXI CCANNNNNNTGG 2 cut(s) 1456, 1566
BstYI RGATCY 1 cut(s) 444
Bsu15I ATCGAT 1 cut(s) 276
Bsu36I CCTNAGG 2 cut(s) 1304, 1376
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 1 cut(s) 1448
BsuTUI ATCGAT 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 1692
BtsCI GGATG 3 cut(s) 364, 808, 1359
BtsI GCAGTG 3 cut(s) 669, 872, 1618
BtsIMutI CAGTG 5 cut(s) 669, 872, 968, 1600, 1618
Cac8I GCNNGC 3 cut(s) 349, 694, 1259
CaiI CAGNNNCTG 3 cut(s) 779, 874, 970
CciI TCATGA 1 cut(s) 612
Cfr13I GGNCC 2 cut(s) 1108, 1743
ClaI ATCGAT 1 cut(s) 276
Csp6I GTAC 2 cut(s) 761, 1273
CviAII CATG 9 cut(s) 136, 348, 613, 764, 826, 1232, 1339, 1489, 1646
CviQI GTAC 2 cut(s) 761, 1273
DdeI CTNAG 8 cut(s) 18, 33, 108, 594, 867, 1304, 1376, 1562
DpnI GATC 5 cut(s) 90, 293, 363, 446, 783
DpnII GATC 5 cut(s) 88, 291, 361, 444, 781
DraI TTTAAA 2 cut(s) 504, 1443
Eam1104I CTCTTC 4 cut(s) 158, 168, 420, 1056
EarI CTCTTC 4 cut(s) 158, 168, 420, 1056
Ecl136II GAGCTC 2 cut(s) 133, 430
Eco130I CCWWGG 1 cut(s) 1449
Eco24I GRGCYC 4 cut(s) 135, 432, 862, 1641
Eco47I GGWCC 2 cut(s) 1108, 1743
Eco53kI GAGCTC 2 cut(s) 133, 430
Eco57I CTGAAG 3 cut(s) 291, 1058, 1283
Eco81I CCTNAGG 2 cut(s) 1304, 1376
Eco88I CYCGRG 1 cut(s) 431
Eco91I GGTNACC 3 cut(s) 806, 902, 1093
EcoICRI GAGCTC 2 cut(s) 133, 430
EcoNI CCTNNNNNAGG 2 cut(s) 962, 1088
EcoO109I RGGNCCY 1 cut(s) 1743
EcoO65I GGTNACC 3 cut(s) 806, 902, 1093
EcoRI GAATTC 1 cut(s) 1589
EcoRII CCWGG 2 cut(s) 54, 1618
EcoT14I CCWWGG 1 cut(s) 1449
EcoT38I GRGCYC 4 cut(s) 135, 432, 862, 1641
ErhI CCWWGG 1 cut(s) 1449
Esp3I CGTCTC 2 cut(s) 104, 1172
FaeI CATG 9 cut(s) 139, 351, 616, 767, 829, 1235, 1342, 1492, 1649
FaqI GGGAC 3 cut(s) 347, 854, 1226
FatI CATG 9 cut(s) 135, 347, 612, 763, 825, 1231, 1338, 1488, 1645
FblI GTMKAC 2 cut(s) 1409, 1503
Fnu4HI GCNGC 5 cut(s) 41, 69, 72, 1047, 1671
FokI GGATG 3 cut(s) 371, 815, 1366
FriOI GRGCYC 4 cut(s) 135, 432, 862, 1641
Fsp4HI GCNGC 5 cut(s) 41, 69, 72, 1047, 1671
FspBI CTAG 7 cut(s) 117, 813, 855, 909, 1298, 1485, 1529
GluI GCNGC 5 cut(s) 41, 69, 72, 1047, 1671
GsaI CCCAGC 1 cut(s) 1644
GsuI CTGGAG 1 cut(s) 1488
HaeIII GGCC 1 cut(s) 1448
Hin1II CATG 9 cut(s) 139, 351, 616, 767, 829, 1235, 1342, 1492, 1649
HinfI GANTC 7 cut(s) 302, 743, 848, 944, 1322, 1472, 1658
HphI GGTGA 5 cut(s) 451, 857, 914, 953, 1471
Hpy166II GTNNAC 4 cut(s) 607, 620, 1410, 1504
Hpy188III TCNNGA 9 cut(s) 433, 613, 755, 855, 1175, 1298, 1467, 1696, 1746
Hpy8I GTNNAC 4 cut(s) 607, 620, 1410, 1504
HpyAV CCTTC 3 cut(s) 196, 262, 1099
HpyCH4III ACNGT 3 cut(s) 845, 1417, 1501
HpyCH4IV ACGT 3 cut(s) 580, 989, 1271
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 113, 247
HpyF3I CTNAG 8 cut(s) 18, 33, 108, 594, 867, 1304, 1376, 1562
HpySE526I ACGT 3 cut(s) 580, 989, 1271
Hsp92II CATG 9 cut(s) 139, 351, 616, 767, 829, 1235, 1342, 1492, 1649
Kzo9I GATC 5 cut(s) 88, 291, 361, 444, 781
LguI GCTCTTC 3 cut(s) 168, 420, 1056
LmnI GCTCC 4 cut(s) 130, 247, 712, 1636
Lsp1109I GCAGC 5 cut(s) 52, 55, 83, 1058, 1657
LweI GCATC 5 cut(s) 649, 679, 766, 1467, 1715
MaeI CTAG 7 cut(s) 117, 813, 855, 909, 1298, 1485, 1529
MaeII ACGT 3 cut(s) 580, 989, 1271
MaeIII GTNAC 5 cut(s) 806, 902, 1093, 1225, 1459
MalI GATC 5 cut(s) 90, 293, 363, 446, 783
MboI GATC 5 cut(s) 88, 291, 361, 444, 781
MflI RGATCY 1 cut(s) 444
MhlI GDGCHC 5 cut(s) 135, 432, 862, 868, 1641
MlyI GAGTC 1 cut(s) 1466
MmeI TCCRAC 2 cut(s) 690, 1270
MspR9I CCNGG 2 cut(s) 56, 1620
Mva1269I GAATGC 2 cut(s) 259, 1048
MvaI CCWGG 2 cut(s) 56, 1620
MwoI GCNNNNNNNGC 3 cut(s) 77, 113, 247
NdeII GATC 5 cut(s) 88, 291, 361, 444, 781
NlaIII CATG 9 cut(s) 139, 351, 616, 767, 829, 1235, 1342, 1492, 1649
NlaIV GGNNCC 3 cut(s) 714, 1638, 1744
NmuCI GTSAC 2 cut(s) 902, 1459
NspI RCATGY 2 cut(s) 351, 1492
PaeI GCATGC 1 cut(s) 351
PaeR7I CTCGAG 1 cut(s) 431
PagI TCATGA 1 cut(s) 612
PciSI GCTCTTC 3 cut(s) 168, 420, 1056
PctI GAATGC 2 cut(s) 259, 1048
PfeI GAWTC 6 cut(s) 302, 743, 848, 944, 1322, 1658
PkrI GCNGC 5 cut(s) 42, 70, 73, 1048, 1672
PleI GAGTC 1 cut(s) 1466
PpsI GAGTC 1 cut(s) 1466
PpuMI RGGWCCY 1 cut(s) 1743
Psp124BI GAGCTC 2 cut(s) 135, 432
Psp1406I AACGTT 1 cut(s) 989
Psp5II RGGWCCY 1 cut(s) 1743
Psp6I CCWGG 2 cut(s) 54, 1618
PspEI GGTNACC 3 cut(s) 806, 902, 1093
PspFI CCCAGC 1 cut(s) 1640
PspGI CCWGG 2 cut(s) 54, 1618
PspN4I GGNNCC 3 cut(s) 714, 1638, 1744
PspPI GGNCC 2 cut(s) 1108, 1743
PspPPI RGGWCCY 1 cut(s) 1743
PstI CTGCAG 4 cut(s) 73, 187, 370, 1675
PstNI CAGNNNCTG 3 cut(s) 779, 874, 970
PsuI RGATCY 1 cut(s) 444
RsaI GTAC 2 cut(s) 762, 1274
RsaNI GTAC 2 cut(s) 761, 1273
SacI GAGCTC 2 cut(s) 135, 432
SapI GCTCTTC 3 cut(s) 168, 420, 1056
SatI GCNGC 5 cut(s) 41, 69, 72, 1047, 1671
Sau3AI GATC 5 cut(s) 88, 291, 361, 444, 781
Sau96I GGNCC 2 cut(s) 1108, 1743
SchI GAGTC 1 cut(s) 1466
ScrFI CCNGG 2 cut(s) 56, 1620
SduI GDGCHC 5 cut(s) 135, 432, 862, 868, 1641
SfaNI GCATC 5 cut(s) 649, 679, 766, 1467, 1715
SfcI CTRYAG 6 cut(s) 69, 183, 366, 1413, 1500, 1671
Sfr274I CTCGAG 1 cut(s) 431
SinI GGWCC 2 cut(s) 1108, 1743
SlaI CTCGAG 1 cut(s) 431
SmlI CTYRAG 3 cut(s) 431, 1203, 1276
SmoI CTYRAG 3 cut(s) 431, 1203, 1276
SpeI ACTAGT 1 cut(s) 1528
SphI GCATGC 1 cut(s) 351
SsiI CCGC 1 cut(s) 1539
SspMI CTAG 7 cut(s) 117, 813, 855, 909, 1298, 1485, 1529
SstI GAGCTC 2 cut(s) 135, 432
StyD4I CCNGG 2 cut(s) 54, 1618
StyI CCWWGG 1 cut(s) 1449
TaaI ACNGT 3 cut(s) 845, 1417, 1501
TaiI ACGT 3 cut(s) 583, 992, 1274
TaqI TCGA 4 cut(s) 276, 432, 1398, 1475
TatI WGTACW 1 cut(s) 760
TfiI GAWTC 6 cut(s) 302, 743, 848, 944, 1322, 1658
TscAI CASTG 5 cut(s) 669, 879, 975, 1600, 1618
TseFI GTSAC 2 cut(s) 902, 1459
TseI GCWGC 5 cut(s) 40, 68, 71, 1046, 1670
Tsp45I GTSAC 2 cut(s) 902, 1459
TspDTI ATGAA 7 cut(s) 405, 780, 1185, 1248, 1329, 1370, 1409
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 5 cut(s) 669, 879, 975, 1600, 1618
VpaK11BI GGWCC 2 cut(s) 1108, 1743
XagI CCTNNNNNAGG 2 cut(s) 962, 1088
XapI RAATTY 4 cut(s) 7, 22, 47, 1589
XbaI TCTAGA 2 cut(s) 854, 1297
XceI RCATGY 2 cut(s) 351, 1492
XhoI CTCGAG 1 cut(s) 431
XmiI GTMKAC 2 cut(s) 1409, 1503
XspI CTAG 7 cut(s) 117, 813, 855, 909, 1298, 1485, 1529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.