Rh6DG322900

CST complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0016565
Physical Location & Seq
Forward (+)
52796088 .. 52797135
1048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG322900.1

Sequence Viewer

Length: 501 bp
ATGGATTATCAACCCCTTCACAACACCCACGTAAAGCTCTTAGCTTTCGACTTGCTCACCCTCACCCAAACCTCCTCCTCCTCCGACCCAACCTCCTTCTCCCGCAACGGCCTCCTCCTCTCACGCGCCGAGGCCGTCGGCACCGTGACCTCACGCGACCTCAAACCCAACAAGTTCCTCCGCTTCACCATCGACGACGGCACCGGCTGCGTCAGCTGCATTCTCTGGCTCAACCACCTCACCTCCCCTTACTTCTCCCGACGAAGCCCACCAGATGTTCGACTCATTGCCTCAATGGCCGCCCGTTTCGCCGCCGACGTCAGCCTCGGAGCAGTGGCCAGAGTGCGCGGGAAAATCTCGAGCTTCAGGGGTGAAATGCAGATCACTGTGACCGATGTTGTGATCGAGAGGGACCCGAATGCGGAGATGTTGCATTGGATTGAGTGCATGAGGTTGGCGCGGAAGGTTTACAATGTGGTTGCTACTACTCCTTCCAAATAG

Protein Analysis

166

Amino Acids

18.46

Weight (kDa)

9.4

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_anti-codon PF01336 46 - 134 1e-08 OB-fold nucleic acid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07130
fragaria_vesca FvH4_2g23151
malus_domestica MD03G1260600.v1.1 MD15G1065600.v1.1
pyrus_communis pycom03g20820
rosa_chinensis RchiOBHm_Chr6g0290411
rosa_laevigata RLG00000012221
rosa_multiflora Rmu_sc0024006.1_g000001
rosa_roxburghii Rroxscaffold_7G00177090
rosa_rugosa Rorug06G0212600
rosa_samantha Rh6BG330000 Rh6CG336000 Rh6DG322900
rosa_wichuraiana Rw6G027870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 321
AccB1I GGYRCC 2 cut(s) 140, 200
AccII CGCG 4 cut(s) 126, 156, 348, 460
AciI CCGC 7 cut(s) 103, 181, 300, 312, 348, 422, 460
AcoI YGGCCR 2 cut(s) 297, 336
AcuI CTGAAG 1 cut(s) 349
AcyI GRCGYC 1 cut(s) 318
AfiI CCNNNNNNNGG 1 cut(s) 421
AluBI AGCT 4 cut(s) 37, 44, 216, 363
AluI AGCT 4 cut(s) 37, 44, 216, 363
Ama87I CYCGRG 1 cut(s) 358
AoxI GGCC 4 cut(s) 109, 132, 297, 336
ApeKI GCWGC 2 cut(s) 207, 216
AspLEI GCGC 3 cut(s) 128, 348, 460
AspS9I GGNCC 1 cut(s) 412
AsuHPI GGTGA 5 cut(s) 49, 55, 178, 232, 383
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 1 cut(s) 412
BalI TGGCCA 1 cut(s) 338
BanI GGYRCC 2 cut(s) 140, 200
BbvI GCAGC 2 cut(s) 194, 203
BccI CCATC 1 cut(s) 197
BceAI ACGGC 3 cut(s) 119, 124, 214
BglI GCCNNNNNGGC 1 cut(s) 296
BisI GCNGC 4 cut(s) 208, 217, 300, 312
BlsI GCNGC 4 cut(s) 209, 218, 301, 313
Bme18I GGWCC 1 cut(s) 412
BmeT110I CYCGRG 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 4 cut(s) 142, 202, 413, 414
BsaAI YACGTR 1 cut(s) 31
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 2 cut(s) 129, 325
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 227, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse118I RCCGGY 1 cut(s) 203
Bse3DI GCAATG 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 129, 325
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMI GCAATG 1 cut(s) 285
BseRI GAGGAG 5 cut(s) 64, 67, 70, 104, 107
BseXI GCAGC 2 cut(s) 194, 203
Bsh1236I CGCG 4 cut(s) 126, 156, 348, 460
BshFI GGCC 4 cut(s) 111, 134, 299, 338
BshNI GGYRCC 2 cut(s) 140, 200
BsiHKCI CYCGRG 1 cut(s) 358
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 425
BslI CCNNNNNNNGG 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 425
BsmI GAATGC 2 cut(s) 219, 424
BsnI GGCC 4 cut(s) 111, 134, 299, 338
BsoBI CYCGRG 1 cut(s) 358
Bsp143I GATC 2 cut(s) 381, 402
BspACI CCGC 7 cut(s) 103, 181, 300, 312, 348, 422, 460
BspANI GGCC 4 cut(s) 111, 134, 299, 338
BspFNI CGCG 4 cut(s) 126, 156, 348, 460
BspLI GGNNCC 4 cut(s) 142, 202, 413, 414
BspT107I GGYRCC 2 cut(s) 140, 200
BsrDI GCAATG 1 cut(s) 285
BsrFI RCCGGY 1 cut(s) 203
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 129, 325
BssMI GATC 2 cut(s) 381, 402
BssNI GRCGYC 1 cut(s) 318
Bst4CI ACNGT 2 cut(s) 145, 388
BstACI GRCGYC 1 cut(s) 318
BstAPI GCANNNNNTGC 1 cut(s) 207
BstBAI YACGTR 1 cut(s) 31
BstDEI CTNAG 1 cut(s) 40
BstFNI CGCG 4 cut(s) 126, 156, 348, 460
BstHHI GCGC 3 cut(s) 128, 348, 460
BstKTI GATC 2 cut(s) 384, 405
BstMBI GATC 2 cut(s) 381, 402
BstMWI GCNNNNNNNGC 5 cut(s) 207, 213, 216, 296, 308
BstUI CGCG 4 cut(s) 126, 156, 348, 460
BstV1I GCAGC 2 cut(s) 194, 203
BsuRI GGCC 4 cut(s) 111, 134, 299, 338
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 2 cut(s) 339, 384
CfoI GCGC 3 cut(s) 128, 348, 460
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 412
CseI GACGC 1 cut(s) 199
CviAII CATG 1 cut(s) 448
DdeI CTNAG 1 cut(s) 40
DpnI GATC 2 cut(s) 383, 404
DpnII GATC 2 cut(s) 381, 402
EaeI YGGCCR 2 cut(s) 297, 336
Eco47I GGWCC 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 358
EcoO109I RGGNCCY 1 cut(s) 412
FaeI CATG 1 cut(s) 451
FaiI YATR 1 cut(s) 449
FaqI GGGAC 1 cut(s) 425
FatI CATG 1 cut(s) 447
FauI CCCGC 2 cut(s) 110, 341
Fnu4HI GCNGC 4 cut(s) 208, 217, 300, 312
Fsp4HI GCNGC 4 cut(s) 208, 217, 300, 312
GlaI GCGC 3 cut(s) 127, 347, 459
GluI GCNGC 4 cut(s) 208, 217, 300, 312
HaeIII GGCC 4 cut(s) 111, 134, 299, 338
HapII CCGG 1 cut(s) 204
HgaI GACGC 1 cut(s) 199
HhaI GCGC 3 cut(s) 128, 348, 460
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 1 cut(s) 451
Hin6I GCGC 3 cut(s) 126, 346, 458
HinP1I GCGC 3 cut(s) 126, 346, 458
HinfI GANTC 1 cut(s) 282
HpaII CCGG 1 cut(s) 204
HphI GGTGA 5 cut(s) 49, 55, 178, 232, 383
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 2 cut(s) 85, 329
Hpy188III TCNNGA 3 cut(s) 258, 358, 406
Hpy8I GTNNAC 1 cut(s) 469
Hpy99I CGWCG 5 cut(s) 140, 197, 200, 264, 320
HpyAV CCTTC 4 cut(s) 26, 106, 457, 501
HpyCH4III ACNGT 2 cut(s) 145, 388
HpyCH4IV ACGT 2 cut(s) 30, 318
HpyCH4V TGCA 4 cut(s) 219, 379, 433, 447
HpyF10VI GCNNNNNNNGC 5 cut(s) 207, 213, 216, 296, 308
HpyF3I CTNAG 1 cut(s) 40
HpySE526I ACGT 2 cut(s) 30, 318
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 1 cut(s) 451
HspAI GCGC 3 cut(s) 126, 346, 458
KflI GGGWCCC 1 cut(s) 412
Kzo9I GATC 2 cut(s) 381, 402
LmnI GCTCC 1 cut(s) 329
LpnPI CCDG 5 cut(s) 211, 217, 285, 352, 352
Lsp1109I GCAGC 2 cut(s) 194, 203
MaeII ACGT 2 cut(s) 30, 318
MaeIII GTNAC 2 cut(s) 145, 388
MalI GATC 2 cut(s) 383, 404
MboI GATC 2 cut(s) 381, 402
MlsI TGGCCA 1 cut(s) 338
MluNI TGGCCA 1 cut(s) 338
MlyI GAGTC 1 cut(s) 276
MmeI TCCRAC 1 cut(s) 108
Mox20I TGGCCA 1 cut(s) 338
MscI TGGCCA 1 cut(s) 338
Msp20I TGGCCA 1 cut(s) 338
MspA1I CMGCKG 1 cut(s) 216
MspI CCGG 1 cut(s) 204
Mva1269I GAATGC 2 cut(s) 219, 424
MvnI CGCG 4 cut(s) 126, 156, 348, 460
MwoI GCNNNNNNNGC 5 cut(s) 207, 213, 216, 296, 308
NdeII GATC 2 cut(s) 381, 402
NlaIII CATG 1 cut(s) 451
NlaIV GGNNCC 4 cut(s) 142, 202, 413, 414
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 2 cut(s) 145, 388
PaeR7I CTCGAG 1 cut(s) 358
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 2 cut(s) 219, 424
PkrI GCNGC 4 cut(s) 209, 218, 301, 313
PleI GAGTC 1 cut(s) 276
PpsI GAGTC 1 cut(s) 276
Ppu21I YACGTR 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 412
Psp5II RGGWCCY 1 cut(s) 412
PspN4I GGNNCC 4 cut(s) 142, 202, 413, 414
PspPI GGNCC 1 cut(s) 412
PspPPI RGGWCCY 1 cut(s) 412
PvuII CAGCTG 1 cut(s) 216
SatI GCNGC 4 cut(s) 208, 217, 300, 312
Sau3AI GATC 2 cut(s) 381, 402
Sau96I GGNCC 1 cut(s) 412
SchI GAGTC 1 cut(s) 276
Sfr274I CTCGAG 1 cut(s) 358
SinI GGWCC 1 cut(s) 412
SlaI CTCGAG 1 cut(s) 358
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
SsiI CCGC 7 cut(s) 103, 181, 300, 312, 348, 422, 460
TaaI ACNGT 2 cut(s) 145, 388
TaiI ACGT 2 cut(s) 33, 321
TaqI TCGA 5 cut(s) 48, 192, 280, 359, 405
TaqII GACCGA 1 cut(s) 407
TauI GCSGC 2 cut(s) 302, 314
TscAI CASTG 2 cut(s) 339, 391
TseFI GTSAC 2 cut(s) 145, 388
TseI GCWGC 2 cut(s) 207, 216
Tsp45I GTSAC 2 cut(s) 145, 388
TspRI CASTG 2 cut(s) 339, 391
VpaK11BI GGWCC 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 358
ZraI GACGTC 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.