Rh7AG433300

von Willebrand factor type A domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014171
Physical Location & Seq
Reverse (-)
58356414 .. 58360147
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG433300.1

Sequence Viewer

Length: 2268 bp
ATGGGTACTGGTTGGAGACGAGCCTTTTGCACCACGATTCGTCGAGATCCATCAGAACACAGAGTTTCAGATCAAAAGCAGAGAAGCCCGAGTCCGAGTCCGAGCCCGAGCCCTAGAACCCGACTGAGCTTCTTCTCCTCCGGTGGTAGCAACCCGTCAACTCCGAAACTACGTTGTAAAACAGGGTCGGAAGCTCTACTTCAGAAATCGAATAGTATGCCTACGAATAATAGTATTGCCGAGAGCCCGAGGGTCCTTGAAATCAAAACCAGCACTCCCAAATCGTCTAATCCGTCTTCTCCTCGATCCCCTCTCAAGCTCTCCCTCTTCAAGAACAGCTTCAAATTTCGAAGTAGCTGTGGAATTTGCTTGAATAGTGTGAAGACTGGGCAAGGCACAGCGATTTACACAGCAGAGTGTAGTCACGCCTTTCATTTTCCTTGCATAGCTTCTTATGTACGGAAGCATGGAAGCCTCGTCTGCCCAGTTTGCAATTCCACATGGAAAGACGTTCCTTTACTGGCTATGCACAAGAGTCCCAGCCCTGGTTCCCACCCGCCAGCCAACGACGCCGTTTCCGCACCTGTCACCCCCAAGCCCAAAGTCGAAGAGAAAAAGGTGTTAGCTGAATCCGCCTCCCCCAGATACACGTTGAAGCCGTACGATGACGACGAGCCACTCTTGTCTCCGACCGTCGGCGGCCGGATTATCCCCATTCCCGAGGCGGAGGAGGAGGAGGAGGAGGAGGACGCCGAGGATGACGTCGAAGAGTTCCAGGGCTTTTTCGTCAATCCCAACACTTCCTCTTCCGTCAAGTATTCTGATGATCCTCAGATAAATAATGGCAGAGATTTCAGGAACAATGTGCAGGTCAGGCTGTTACCGGAGGCTGCTTTGTTGTCCTCCGGCCGGGGTTTCGAGACCTACGCCGTGGCTTTGACAGTCAAGGCTCCGCCGGCGCCGGCTCGTCAGACTACCTGCTGCGCGTCGATTCTGGACCCTTCCCACCGTGCCCCGATCGATTTGGTGACGGTGCTCGACGTCAGCGGCAGCATGACCGGCGGGAAGCTTCAGATGCTGAAACGCGCCATGCGTTTGGTCATATCCTCGCTCGGCTCGGCTGATCGGCTCTCCATCGTGGCTTTCTCGGCTTCGCCAAAGAGACTGATGCCTTTGAAGAGAATGACGGCTCATGGCCAGCGCGCGGCTCGGCGTATCGTTGACCGGCTGGTCTGCGGTCAAGGGAGTAGCGTTGGTGAGGCCTTGATGAAGGCCACGAAGGTTCTGGAAGACCGGAGGGAGAGGAATCCAGTGGCGAGCATCATGCTCTTATCGGACGGTCAGGACGAGAGGGTCAACAACTCCGGCTCAACCACTCAACGTCAGGTCTCAAACGACGTGTCGTCCACCCGGTTCGCCCACATTGAGATTCCGGTTCATGCTTTCGGGTTCGGGCAGAACGCCGGCTACTGCCAGGAGCCGGCTGAGGACGCCTTTGCCAAATGCGTCGGGGGTTTACTAAGCGTCGTCGTTCAGGACTTGAGAGTTCAGCTCGGCTTCTCATCCGGCTCGGCTCCCGCTGAGATAACCGCCATTTATTCCTGCAACGGACGGCCCACCGTGCACGGCTCGTCTTCGATTCGGCTCGGAGATTTGTACGCCGAGGAAGAGAGGGAGTTGCTCGTTGAGCTTCGTATCCCTACATCGGCTGCTGGGACCCACCATGTGATGTCAGTTCGGTGCTTATACAAAGACCCGGCGACGCAAGAAGTTGTCTACGGCAAGGAGCAGGGACTAGTAGTGCCCCTCACGCAGTCAGCCGTCCGATCGCTGTCCTTGAATCCGAAGATCCAACGGTTGAGAAGACTTTTCATGACTACGCGCGCTGTAGCGGAGTCACGGAGGCTGGTTGAGCACAATGACTTCCAAAGTGCACATCACTTGCTAGCATCGACTCGAGCTCTGCTCATGCAGTCCGGCTTGTCTTCGGCTGATGAGTACGTTCGGACATTGGAGGCTCAGCTGGCGGAGTTGCATTGGAAGAGGCAGAATCAGCTGGAGGAGCAGCAGCAGCAGATGATGATAATGCAGCGGCGGAGAGTAAGCGAGAGAGAAATGGCGGCGTCGGCGGCTGTCGATGAAAACGGAGAGCCGCTGACGCCGACTTCGGCGTGGAGAGCCGCGGAGAAGCTGGCTAAGGTAGCTATGATGAAGAAGTCGTTGAATAGGGTCAGTGACTTGCACGGCTTTGAAAATGCTAGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA_2 zf-RING_11 zf-RING_2

Protein Analysis

755

Amino Acids

82.69

Weight (kDa)

9.07

Isoelectric Point (pI)

54.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 119 - 165 6e-09 Ring finger domain
zf-RING_11 PF17123 120 - 149 5.9e-09 RING-like zinc finger
zf-C3HC4 PF00097 120 - 164 2.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 122 - 165 2.6e-06 RING-H2 zinc finger domain
VWA PF00092 341 - 486 6.4e-15 von Willebrand factor type A domain
VWA_3 PF13768 341 - 486 2.3e-12 von Willebrand factor type A domain
VWA_2 PF13519 342 - 448 9.2e-21 von Willebrand factor type A domain
WAV3_C PF25243 614 - 688 1.1e-34 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014171)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49665
fragaria_vesca FvH4_5g33780 FvH4_5g33780
malus_domestica MD08G1207200.v1.1 MD15G1391600.v1.1
prunus_persica Prupe.1G540900_v2.0.a1
pyrus_communis pycom08g17950
rosa_chinensis RchiOBHm_Chr7g0233091
rosa_laevigata RLG00000001300
rosa_multiflora Rmu_sc0016344.1_g000006
rosa_roxburghii Rroxscaffold_3G00227690
rosa_rugosa Rorug07G0278100
rosa_samantha Rh7AG433300 Rh7BG406100 Rh7CG452700 Rh7DG421900
rosa_wichuraiana Rw7G035730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1229, 1352
AatII GACGTC 2 cut(s) 765, 1044
Acc36I ACCTGC 2 cut(s) 859, 986
AccB1I GGYRCC 1 cut(s) 958
AccI GTMKAC 1 cut(s) 1776
AccII CGCG 7 cut(s) 986, 1086, 1203, 1205, 1882, 1884, 2183
AclWI GGATC 4 cut(s) 41, 300, 821, 1843
AcoI YGGCCR 3 cut(s) 700, 907, 1195
AcsI RAATTY 2 cut(s) 344, 363
AcuI CTGAAG 2 cut(s) 185, 1055
AcyI GRCGYC 8 cut(s) 570, 750, 762, 959, 1041, 1491, 2123, 2159
AdeI CACNNNGTG 1 cut(s) 1726
AfaI GTAC 5 cut(s) 7, 459, 662, 1658, 2000
AfiI CCNNNNNNNGG 6 cut(s) 545, 695, 909, 1204, 1480, 1705
AflIII ACRYGT 2 cut(s) 648, 1398
AgsI TTSAA 9 cut(s) 260, 331, 343, 373, 655, 1177, 1840, 2224, 2252
AhdI GACNNNNNGTC 1 cut(s) 1402
AhlI ACTAGT 1 cut(s) 1795
AjiI CACGTC 1 cut(s) 1399
AjnI CCWGG 3 cut(s) 544, 774, 1473
Alw21I GWGCWC 5 cut(s) 1038, 1626, 1917, 1936, 1963
Alw26I GTCTC 5 cut(s) 10, 690, 914, 1156, 1393
Alw44I GTGCAC 2 cut(s) 1622, 1932
AlwI GGATC 4 cut(s) 41, 300, 821, 1843
AlwNI CAGNNNCTG 1 cut(s) 1078
Ama87I CYCGRG 5 cut(s) 88, 106, 247, 719, 1956
AoxI GGCC 6 cut(s) 700, 907, 1195, 1260, 1272, 1613
ApaLI GTGCAC 2 cut(s) 1622, 1932
ApeKI GCWGC 8 cut(s) 890, 981, 1050, 1709, 2065, 2068, 2071, 2089
ApoI RAATTY 2 cut(s) 344, 363
ArsI GACNNNNNNTTYG 6 cut(s) 9, 41, 1385, 1417, 2149, 2181
Asp700I GAANNNNTTC 1 cut(s) 338
AspLEI GCGC 7 cut(s) 961, 986, 1088, 1203, 1205, 1884, 1886
AspS9I GGNCC 4 cut(s) 253, 997, 1614, 1716
AsuC2I CCSGG 3 cut(s) 911, 1411, 1757
AsuHPI GGTGA 3 cut(s) 580, 1039, 1268
AsuII TTCGAA 1 cut(s) 349
AsuNHI GCTAGC 1 cut(s) 1945
AvaI CYCGRG 5 cut(s) 88, 106, 247, 719, 1956
AvaII GGWCC 3 cut(s) 253, 997, 1716
BaeGI GKGCMC 4 cut(s) 1015, 1626, 1806, 1936
BalI TGGCCA 1 cut(s) 1197
BanI GGYRCC 1 cut(s) 958
BanII GRGCYC 4 cut(s) 107, 113, 248, 1963
BbsI GAAGAC 6 cut(s) 288, 389, 1296, 1626, 1870, 1977
Bbv12I GWGCWC 5 cut(s) 1038, 1626, 1917, 1936, 1963
BbvCI CCTCAGC 1 cut(s) 1485
BbvI GCAGC 8 cut(s) 877, 968, 1062, 1696, 2077, 2080, 2083, 2101
BccI CCATC 2 cut(s) 58, 1142
BceAI ACGGC 9 cut(s) 557, 643, 914, 1203, 1628, 1642, 1795, 1805, 2260
BcgI CGANNNNNNTGC 6 cut(s) 9, 43, 199, 233, 1306, 1340
BciT130I CCWGG 3 cut(s) 546, 776, 1475
BciVI GTATCC 1 cut(s) 1706
BcnI CCSGG 3 cut(s) 911, 1411, 1757
BcoDI GTCTC 5 cut(s) 10, 690, 914, 1156, 1393
BcuI ACTAGT 1 cut(s) 1795
BfaI CTAG 4 cut(s) 114, 1796, 1946, 2259
BfmI CTRYAG 1 cut(s) 1887
BfoI RGCGCY 1 cut(s) 962
BfuAI ACCTGC 2 cut(s) 859, 986
BfuI GTATCC 1 cut(s) 1706
BlpI GCTNAGC 1 cut(s) 2019
Bme1390I CCNGG 6 cut(s) 546, 776, 911, 1411, 1475, 1757
Bme18I GGWCC 3 cut(s) 253, 997, 1716
BmeRI GACNNNNNGTC 1 cut(s) 1402
BmeT110I CYCGRG 5 cut(s) 88, 106, 247, 719, 1956
BmgBI CACGTC 1 cut(s) 1399
BmgT120I GGNCC 4 cut(s) 253, 997, 1614, 1716
BmiI GGNNCC 9 cut(s) 254, 550, 951, 960, 999, 1479, 1575, 1717, 1718
BmrFI CCNGG 6 cut(s) 546, 776, 911, 1411, 1475, 1757
BmrI ACTGGG 2 cut(s) 396, 479
BmsI GCATC 4 cut(s) 1065, 1158, 1329, 1958
BmtI GCTAGC 1 cut(s) 1949
BmuI ACTGGG 2 cut(s) 396, 479
BpiI GAAGAC 6 cut(s) 288, 389, 1296, 1626, 1870, 1977
BplI GAGNNNNNCTC 4 cut(s) 1536, 1568, 1950, 1982
BpmI CTGGAG 1 cut(s) 2078
Bpu10I CCTNAGC 2 cut(s) 1485, 2196
Bpu1102I GCTNAGC 1 cut(s) 2019
Bpu14I TTCGAA 1 cut(s) 349
BpuEI CTTGAG 2 cut(s) 299, 1561
BpuMI CCSGG 3 cut(s) 911, 1411, 1757
Bsa29I ATCGAT 1 cut(s) 1020
BsaHI GRCGYC 8 cut(s) 570, 750, 762, 959, 1041, 1491, 2123, 2159
BsaI GGTCTC 2 cut(s) 914, 1393
BsaJI CCNNGG 9 cut(s) 248, 544, 720, 753, 775, 910, 930, 1662, 2181
BsaWI WCCGGW 4 cut(s) 140, 883, 1293, 1432
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 6 cut(s) 545, 695, 909, 1204, 1480, 1705
Bse118I RCCGGY 6 cut(s) 955, 961, 1058, 1224, 1463, 1480
Bse1I ACTGG 5 cut(s) 13, 391, 485, 525, 1310
BseBI CCWGG 3 cut(s) 546, 776, 1475
BseCI ATCGAT 1 cut(s) 1020
BseDI CCNNGG 9 cut(s) 248, 544, 720, 753, 775, 910, 930, 1662, 2181
BseGI GGATG 2 cut(s) 763, 1562
BseLI CCNNNNNNNGG 6 cut(s) 545, 695, 909, 1204, 1480, 1705
BseMII CTCAG 5 cut(s) 116, 845, 1476, 1572, 2033
BseNI ACTGG 5 cut(s) 13, 391, 485, 525, 1310
BsePI GCGCGC 2 cut(s) 1201, 1882
BseRI GAGGAG 9 cut(s) 127, 291, 743, 746, 749, 752, 755, 758, 2075
BseSI GKGCMC 4 cut(s) 1015, 1626, 1806, 1936
BseX3I CGGCCG 2 cut(s) 700, 907
BseXI GCAGC 8 cut(s) 877, 968, 1062, 1696, 2077, 2080, 2083, 2101
BseYI CCCAGC 2 cut(s) 539, 1712
BsgI GTGCAG 1 cut(s) 887
Bsh1236I CGCG 7 cut(s) 986, 1086, 1203, 1205, 1882, 1884, 2183
Bsh1285I CGRYCG 5 cut(s) 693, 703, 910, 1020, 1829
BshFI GGCC 6 cut(s) 702, 909, 1197, 1262, 1274, 1615
BshNI GGYRCC 1 cut(s) 958
BshVI ATCGAT 1 cut(s) 1020
BsiEI CGRYCG 5 cut(s) 693, 703, 910, 1020, 1829
BsiHKAI GWGCWC 5 cut(s) 1038, 1626, 1917, 1936, 1963
BsiHKCI CYCGRG 5 cut(s) 88, 106, 247, 719, 1956
BsiWI CGTACG 1 cut(s) 660
BslFI GGGAC 3 cut(s) 522, 1729, 1806
BslI CCNNNNNNNGG 6 cut(s) 545, 695, 909, 1204, 1480, 1705
BsmAI GTCTC 5 cut(s) 10, 690, 914, 1156, 1393
BsmBI CGTCTC 1 cut(s) 10
BsmFI GGGAC 3 cut(s) 522, 1729, 1806
BsnI GGCC 6 cut(s) 702, 909, 1197, 1262, 1274, 1615
Bso31I GGTCTC 2 cut(s) 914, 1393
BsoBI CYCGRG 5 cut(s) 88, 106, 247, 719, 1956
Bsp119I TTCGAA 1 cut(s) 349
Bsp143I GATC 8 cut(s) 46, 70, 305, 826, 1017, 1123, 1826, 1848
Bsp1720I GCTNAGC 1 cut(s) 2019
BspANI GGCC 6 cut(s) 702, 909, 1197, 1262, 1274, 1615
BspCNI CTCAG 5 cut(s) 117, 844, 1477, 1573, 2032
BspDI ATCGAT 1 cut(s) 1020
BspFNI CGCG 7 cut(s) 986, 1086, 1203, 1205, 1882, 1884, 2183
BspHI TCATGA 1 cut(s) 1872
BspLI GGNNCC 9 cut(s) 254, 550, 951, 960, 999, 1479, 1575, 1717, 1718
BspMI ACCTGC 2 cut(s) 859, 986
BspOI GCTAGC 1 cut(s) 1949
BspPI GGATC 4 cut(s) 41, 300, 821, 1843
BspT104I TTCGAA 1 cut(s) 349
BspT107I GGYRCC 1 cut(s) 958
BspTNI GGTCTC 2 cut(s) 914, 1393
BsrFI RCCGGY 6 cut(s) 955, 961, 1058, 1224, 1463, 1480
BsrI ACTGG 5 cut(s) 13, 391, 485, 525, 1310
BssAI RCCGGY 6 cut(s) 955, 961, 1058, 1224, 1463, 1480
BssECI CCNNGG 9 cut(s) 248, 544, 720, 753, 775, 910, 930, 1662, 2181
BssHII GCGCGC 2 cut(s) 1201, 1882
BssMI GATC 8 cut(s) 46, 70, 305, 826, 1017, 1123, 1826, 1848
BssNI GRCGYC 8 cut(s) 570, 750, 762, 959, 1041, 1491, 2123, 2159
Bst2UI CCWGG 3 cut(s) 546, 776, 1475
Bst4CI ACNGT 7 cut(s) 694, 943, 1010, 1033, 1340, 1621, 1857
Bst6I CTCTTC 7 cut(s) 332, 603, 762, 811, 1172, 1662, 2036
BstACI GRCGYC 8 cut(s) 570, 750, 762, 959, 1041, 1491, 2123, 2159
BstBI TTCGAA 1 cut(s) 349
BstDEI CTNAG 7 cut(s) 125, 831, 1485, 1520, 1581, 2019, 2196
BstDSI CCRYGG 2 cut(s) 930, 2181
BstF5I GGATG 2 cut(s) 763, 1562
BstFNI CGCG 7 cut(s) 986, 1086, 1203, 1205, 1882, 1884, 2183
BstH2I RGCGCY 1 cut(s) 962
BstHHI GCGC 7 cut(s) 961, 986, 1088, 1203, 1205, 1884, 1886
BstKTI GATC 8 cut(s) 49, 73, 308, 829, 1020, 1126, 1829, 1851
BstMAI GTCTC 5 cut(s) 10, 690, 914, 1156, 1393
BstMBI GATC 8 cut(s) 46, 70, 305, 826, 1017, 1123, 1826, 1848
BstMCI CGRYCG 5 cut(s) 693, 703, 910, 1020, 1829
BstNI CCWGG 3 cut(s) 546, 776, 1475
BstSCI CCNGG 6 cut(s) 544, 774, 909, 1409, 1473, 1755
BstSFI CTRYAG 1 cut(s) 1887
BstSLI GKGCMC 4 cut(s) 1015, 1626, 1806, 1936
BstUI CGCG 7 cut(s) 986, 1086, 1203, 1205, 1882, 1884, 2183
BstV1I GCAGC 8 cut(s) 877, 968, 1062, 1696, 2077, 2080, 2083, 2101
BstV2I GAAGAC 6 cut(s) 288, 389, 1296, 1626, 1870, 1977
BstX2I RGATCY 2 cut(s) 46, 1848
BstXI CCANNNNNNTGG 1 cut(s) 1096
BstYI RGATCY 2 cut(s) 46, 1848
BstZI CGGCCG 2 cut(s) 700, 907
Bsu15I ATCGAT 1 cut(s) 1020
BsuI GTATCC 1 cut(s) 1706
BsuRI GGCC 6 cut(s) 702, 909, 1197, 1262, 1274, 1615
BsuTUI ATCGAT 1 cut(s) 1020
BtgI CCRYGG 2 cut(s) 930, 2181
BtrI CACGTC 1 cut(s) 1399
BtsCI GGATG 2 cut(s) 763, 1562
BtsIMutI CAGTG 2 cut(s) 1317, 2239
BveI ACCTGC 2 cut(s) 859, 986
CaiI CAGNNNCTG 1 cut(s) 1078
CciI TCATGA 1 cut(s) 1872
CfoI GCGC 7 cut(s) 961, 986, 1088, 1203, 1205, 1884, 1886
Cfr10I RCCGGY 6 cut(s) 955, 961, 1058, 1224, 1463, 1480
Cfr13I GGNCC 4 cut(s) 253, 997, 1614, 1716
Cfr42I CCGCGG 1 cut(s) 2184
ClaI ATCGAT 1 cut(s) 1020
CseI GACGC 9 cut(s) 578, 758, 975, 1495, 1499, 1513, 1771, 2112, 2167
Csp6I GTAC 5 cut(s) 6, 458, 661, 1657, 1999
CviQI GTAC 5 cut(s) 6, 458, 661, 1657, 1999
DdeI CTNAG 7 cut(s) 125, 831, 1485, 1520, 1581, 2019, 2196
DinI GGCGCC 1 cut(s) 960
DpnI GATC 8 cut(s) 48, 72, 307, 828, 1019, 1125, 1828, 1850
DpnII GATC 8 cut(s) 46, 70, 305, 826, 1017, 1123, 1826, 1848
DraIII CACNNNGTG 1 cut(s) 1726
DrdI GACNNNNNNGTC 2 cut(s) 1229, 1352
DriI GACNNNNNGTC 1 cut(s) 1402
DseDI GACNNNNNNGTC 2 cut(s) 1229, 1352
EaeI YGGCCR 3 cut(s) 700, 907, 1195
EagI CGGCCG 2 cut(s) 700, 907
Eam1104I CTCTTC 7 cut(s) 332, 603, 762, 811, 1172, 1662, 2036
Eam1105I GACNNNNNGTC 1 cut(s) 1402
EarI CTCTTC 7 cut(s) 332, 603, 762, 811, 1172, 1662, 2036
EciI GGCGGA 5 cut(s) 622, 740, 942, 2042, 2110
Ecl136II GAGCTC 1 cut(s) 1961
EclXI CGGCCG 2 cut(s) 700, 907
Eco147I AGGCCT 1 cut(s) 1262
Eco24I GRGCYC 4 cut(s) 107, 113, 248, 1963
Eco31I GGTCTC 2 cut(s) 914, 1393
Eco47I GGWCC 3 cut(s) 253, 997, 1716
Eco52I CGGCCG 2 cut(s) 700, 907
Eco53kI GAGCTC 1 cut(s) 1961
Eco57I CTGAAG 2 cut(s) 185, 1055
Eco88I CYCGRG 5 cut(s) 88, 106, 247, 719, 1956
EcoICRI GAGCTC 1 cut(s) 1961
EcoO109I RGGNCCY 2 cut(s) 253, 1716
EcoRII CCWGG 3 cut(s) 544, 774, 1473
EcoT38I GRGCYC 4 cut(s) 107, 113, 248, 1963
EgeI GGCGCC 1 cut(s) 960
EheI GGCGCC 1 cut(s) 960
Esp3I CGTCTC 1 cut(s) 10
FalI AAGNNNNNCTT 4 cut(s) 183, 215, 323, 355
FaqI GGGAC 3 cut(s) 522, 1729, 1806
FauI CCCGC 3 cut(s) 564, 1055, 1585
FblI GTMKAC 1 cut(s) 1776
FokI GGATG 2 cut(s) 770, 1549
FriOI GRGCYC 4 cut(s) 107, 113, 248, 1963
FspBI CTAG 4 cut(s) 114, 1796, 1946, 2259
GlaI GCGC 7 cut(s) 960, 985, 1087, 1202, 1204, 1883, 1885
GsaI CCCAGC 2 cut(s) 543, 1716
GsuI CTGGAG 1 cut(s) 2078
HaeII RGCGCY 1 cut(s) 962
HaeIII GGCC 6 cut(s) 702, 909, 1197, 1262, 1274, 1615
HgaI GACGC 9 cut(s) 578, 758, 975, 1495, 1499, 1513, 1771, 2112, 2167
HhaI GCGC 7 cut(s) 961, 986, 1088, 1203, 1205, 1884, 1886
Hin1I GRCGYC 8 cut(s) 570, 750, 762, 959, 1041, 1491, 2123, 2159
Hin6I GCGC 7 cut(s) 959, 984, 1086, 1201, 1203, 1882, 1884
HinP1I GCGC 7 cut(s) 959, 984, 1086, 1201, 1203, 1882, 1884
HincII GTYRAC 3 cut(s) 159, 1222, 1357
HindII GTYRAC 3 cut(s) 159, 1222, 1357
HindIII AAGCTT 1 cut(s) 1067
HphI GGTGA 3 cut(s) 580, 1039, 1268
Hpy166II GTNNAC 8 cut(s) 159, 1222, 1357, 1407, 1517, 1624, 1777, 1934
Hpy8I GTNNAC 8 cut(s) 159, 1222, 1357, 1407, 1517, 1624, 1777, 1934
HpyAV CCTTC 3 cut(s) 1011, 1264, 1273
HpyCH4III ACNGT 7 cut(s) 694, 943, 1010, 1033, 1340, 1621, 1857
HpyCH4IV ACGT 8 cut(s) 172, 510, 650, 762, 1041, 1381, 1398, 2001
HpyF3I CTNAG 7 cut(s) 125, 831, 1485, 1520, 1581, 2019, 2196
HpySE526I ACGT 8 cut(s) 172, 510, 650, 762, 1041, 1381, 1398, 2001
Hsp92I GRCGYC 8 cut(s) 570, 750, 762, 959, 1041, 1491, 2123, 2159
HspAI GCGC 7 cut(s) 959, 984, 1086, 1201, 1203, 1882, 1884
KasI GGCGCC 1 cut(s) 958
KflI GGGWCCC 1 cut(s) 1716
KroI GCCGGC 4 cut(s) 955, 961, 1463, 1480
KroNI GCCGGC 4 cut(s) 957, 963, 1465, 1482
KspI CCGCGG 1 cut(s) 2184
Kzo9I GATC 8 cut(s) 46, 70, 305, 826, 1017, 1123, 1826, 1848
LmnI GCTCC 5 cut(s) 955, 1477, 1579, 1786, 2062
Lsp1109I GCAGC 8 cut(s) 877, 968, 1062, 1696, 2077, 2080, 2083, 2101
LweI GCATC 4 cut(s) 1065, 1158, 1329, 1958
MaeI CTAG 4 cut(s) 114, 1796, 1946, 2259
MaeII ACGT 8 cut(s) 172, 510, 650, 762, 1041, 1381, 1398, 2001
MaeIII GTNAC 6 cut(s) 422, 586, 879, 1027, 1896, 2234
MalI GATC 8 cut(s) 48, 72, 307, 828, 1019, 1125, 1828, 1850
MboI GATC 8 cut(s) 46, 70, 305, 826, 1017, 1123, 1826, 1848
MflI RGATCY 2 cut(s) 46, 1848
MlsI TGGCCA 1 cut(s) 1197
MluCI AATT 3 cut(s) 344, 363, 493
MluNI TGGCCA 1 cut(s) 1197
Mly113I GGCGCC 1 cut(s) 959
MlyI GAGTC 5 cut(s) 100, 106, 544, 1904, 1948
MmeI TCCRAC 3 cut(s) 168, 713, 1876
Mox20I TGGCCA 1 cut(s) 1197
MreI CGCCGGCG 1 cut(s) 955
MroNI GCCGGC 4 cut(s) 955, 961, 1463, 1480
MroXI GAANNNNTTC 1 cut(s) 338
MscI TGGCCA 1 cut(s) 1197
MseI TTAA 1 cut(s) 2266
Msp20I TGGCCA 1 cut(s) 1197
MspA1I CMGCKG 7 cut(s) 1047, 1580, 2023, 2056, 2092, 2155, 2183
MspR9I CCNGG 6 cut(s) 546, 776, 911, 1411, 1475, 1757
MvaI CCWGG 3 cut(s) 546, 776, 1475
MvnI CGCG 7 cut(s) 986, 1086, 1203, 1205, 1882, 1884, 2183
NaeI GCCGGC 4 cut(s) 957, 963, 1465, 1482
NarI GGCGCC 1 cut(s) 959
NciI CCSGG 3 cut(s) 911, 1411, 1757
NdeII GATC 8 cut(s) 46, 70, 305, 826, 1017, 1123, 1826, 1848
NgoMIV GCCGGC 4 cut(s) 955, 961, 1463, 1480
NheI GCTAGC 1 cut(s) 1945
NlaIV GGNNCC 9 cut(s) 254, 550, 951, 960, 999, 1479, 1575, 1717, 1718
NmeAIII GCCGAG 9 cut(s) 265, 778, 1092, 1097, 1127, 1189, 1533, 1550, 1687
NmuCI GTSAC 5 cut(s) 422, 586, 1027, 1896, 2234
NspV TTCGAA 1 cut(s) 349
PaeR7I CTCGAG 1 cut(s) 1956
PagI TCATGA 1 cut(s) 1872
PauI GCGCGC 2 cut(s) 1201, 1882
PceI AGGCCT 1 cut(s) 1262
PcsI WCGNNNNNNNCGW 5 cut(s) 290, 656, 669, 1090, 1344
PdiI GCCGGC 4 cut(s) 957, 963, 1465, 1482
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 8 cut(s) 37, 629, 991, 1306, 1429, 1639, 1840, 2050
Pfl23II CGTACG 1 cut(s) 660
Ple19I CGATCG 2 cut(s) 1020, 1829
PleI GAGTC 5 cut(s) 99, 105, 543, 1903, 1948
PluTI GGCGCC 1 cut(s) 962
PpsI GAGTC 5 cut(s) 99, 105, 543, 1903, 1948
PpuMI RGGWCCY 2 cut(s) 253, 1716
Psp124BI GAGCTC 1 cut(s) 1963
Psp5II RGGWCCY 2 cut(s) 253, 1716
Psp6I CCWGG 3 cut(s) 544, 774, 1473
PspFI CCCAGC 2 cut(s) 539, 1712
PspGI CCWGG 3 cut(s) 544, 774, 1473
PspLI CGTACG 1 cut(s) 660
PspN4I GGNNCC 9 cut(s) 254, 550, 951, 960, 999, 1479, 1575, 1717, 1718
PspPI GGNCC 4 cut(s) 253, 997, 1614, 1716
PspPPI RGGWCCY 2 cut(s) 253, 1716
PspXI VCTCGAGB 1 cut(s) 1956
PsrI GAACNNNNNNTAC 2 cut(s) 1451, 1483
PstNI CAGNNNCTG 1 cut(s) 1078
PsuI RGATCY 2 cut(s) 46, 1848
PteI GCGCGC 2 cut(s) 1201, 1882
PvuI CGATCG 2 cut(s) 1020, 1829
PvuII CAGCTG 2 cut(s) 2023, 2056
RsaI GTAC 5 cut(s) 7, 459, 662, 1658, 2000
RsaNI GTAC 5 cut(s) 6, 458, 661, 1657, 1999
SacI GAGCTC 1 cut(s) 1963
SacII CCGCGG 1 cut(s) 2184
SaqAI TTAA 1 cut(s) 2266
Sau3AI GATC 8 cut(s) 46, 70, 305, 826, 1017, 1123, 1826, 1848
Sau96I GGNCC 4 cut(s) 253, 997, 1614, 1716
SchI GAGTC 5 cut(s) 100, 106, 544, 1904, 1948
ScrFI CCNGG 6 cut(s) 546, 776, 911, 1411, 1475, 1757
SfaNI GCATC 4 cut(s) 1065, 1158, 1329, 1958
SfcI CTRYAG 1 cut(s) 1887
SfoI GGCGCC 1 cut(s) 960
Sfr274I CTCGAG 1 cut(s) 1956
Sfr303I CCGCGG 1 cut(s) 2184
SfuI TTCGAA 1 cut(s) 349
SgrAI CRCCGGYG 1 cut(s) 955
SgrBI CCGCGG 1 cut(s) 2184
SinI GGWCC 3 cut(s) 253, 997, 1716
SlaI CTCGAG 1 cut(s) 1956
SmlI CTYRAG 3 cut(s) 314, 1540, 1956
SmoI CTYRAG 3 cut(s) 314, 1540, 1956
SpeI ACTAGT 1 cut(s) 1795
Sse9I AATT 3 cut(s) 344, 363, 493
SseBI AGGCCT 1 cut(s) 1262
SspDI GGCGCC 1 cut(s) 958
SspMI CTAG 4 cut(s) 114, 1796, 1946, 2259
SstI GAGCTC 1 cut(s) 1963
StuI AGGCCT 1 cut(s) 1262
StyD4I CCNGG 6 cut(s) 544, 774, 909, 1409, 1473, 1755
TaaI ACNGT 7 cut(s) 694, 943, 1010, 1033, 1340, 1621, 1857
TaiI ACGT 8 cut(s) 175, 513, 653, 765, 1044, 1384, 1401, 2004
TasI AATT 3 cut(s) 344, 363, 493
TauI GCSGC 8 cut(s) 702, 1050, 1208, 2095, 2123, 2132, 2155, 2183
TfiI GAWTC 8 cut(s) 37, 629, 991, 1306, 1429, 1639, 1840, 2050
Tru1I TTAA 1 cut(s) 2266
Tru9I TTAA 1 cut(s) 2266
TscAI CASTG 2 cut(s) 1317, 2239
TseFI GTSAC 5 cut(s) 422, 586, 1027, 1896, 2234
TseI GCWGC 8 cut(s) 890, 981, 1050, 1709, 2065, 2068, 2071, 2089
Tsp45I GTSAC 5 cut(s) 422, 586, 1027, 1896, 2234
TspDTI ATGAA 6 cut(s) 422, 1283, 1427, 1861, 2154, 2225
TspGWI ACGGA 6 cut(s) 282, 475, 799, 1623, 1915, 2160
TspRI CASTG 2 cut(s) 1317, 2239
VneI GTGCAC 2 cut(s) 1622, 1932
VpaK11BI GGWCC 3 cut(s) 253, 997, 1716
XapI RAATTY 2 cut(s) 344, 363
XcmI CCANNNNNNNNNTGG 1 cut(s) 1282
XhoI CTCGAG 1 cut(s) 1956
XmiI GTMKAC 1 cut(s) 1776
XmnI GAANNNNTTC 1 cut(s) 338
XspI CTAG 4 cut(s) 114, 1796, 1946, 2259
ZraI GACGTC 2 cut(s) 763, 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.