Rh7CG452700

von Willebrand factor type A domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0014171
Physical Location & Seq
Reverse (-)
59546100 .. 59549830
3731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG452700.1

Sequence Viewer

Length: 2265 bp
ATGGGTACTGGTTGGAGACGAGCCTTTTGCACCACGATTCGTCGAGATCCATCAGAACACAGAGTTTCAGATCAAAAGCAGAGAAGCCCGAGTCCGAGTCCGAGCCCGAGCCCTAGAACCCGACTGAGCTTCTTCTCCTCCGGTGGTAGCAACCCGTCAACTCCGAAACTACGTTGTAAAACAGGGTCGGAAGCTCTACTTCAGAAATCGAATAGTATGCCTACGAATAATAGTATTGCCGAGAGCCCGAGGGTCCTTGAAATCAAAACCAGCACTCCCAAATCGTCTAATCCGTCTTCTCCTCGATCCCCTCTCAAGCTCTCCCTCTTCAAGAACAGCTTCAAATTTCGAAGTAGCTGTGGAATTTGCTTGAATAGTGTGAAGACTGGGCAAGGCACAGCGATTTACACAGCAGAGTGTAGTCACGCCTTTCATTTTCCTTGCATAGCTTCTTATGTACGGAAGCATGGAAGCCTCGTCTGCCCAGTTTGCAATTCCACATGGAAAGACGTTCCTTTACTGGCTATGCACAAGAGTCCCAGCCCTGATTCCCACCCGCCAGCCAACGACGCCGTTTCCGCACCTGTCACCCCCAAGCCCAAAGTCGAAGAGAAAAAGGTGTTAGCTGAATCCGCCTCCCCCAGATACACGTTGAAGCCATACGATGACGACGAGCCACTCTTGTCTCCGACCGTCGGCGGCCGGATTATCCCCATTCCCGAGGCGGAGGAGGAGGAGGAGGAGGACGCCGAGGATGACGTCGAAGAGTTCCAGGGCTTTTTCGTCAATCCCAACACTTCCTCTTCCGTCAAGTATTCTGATGATCCTCAGATAAATAATGGCAGAGATTTCAGGAACAATGTGCAGGTCAGGCTGTTACCGGAGGCTGCTTTGTTGTCCTCCGGCCGGGGTTTCGAGACCTACGCCGTGGCTTTGACAGTCAAGGCTCCGCCGGCGCCGGCTCGTCAGACTACCTGCTGCGCGTCGATTCTGGACCCTTCCCACCGTGCCCCGATCGATTTGGTGACGGTGCTCGACGTCAGCGGCAGCATGACCGGCGGGAAGCTTCAGATGCTGAAACGCGCCATGCGTTTGGTCATATCCTCGCTCGGCTCGGCTGATCGGCTCTCCATCGTGGCTTTCTCGGCTTCGCCAAAGAGACTGATGCCTTTGAAGAGAATGACGGCTCATGGCCAGCGCGCGGCTCGGCGCATCGTTGACCGGCTGGTCTGCGGTCAAGGGAGTAGCGTTGGTGAGGCCTTGATGAAGGCCACGAAGGTTCTGGAAGACCGGAGGGAGAGGAATCCAGTGGCGAGCATCATGCTCTTATCGGACGGTCAGGACGAGAGGGTCAACAACTCCGGCTCAACCACTCAACGTCAGGTCTCAAACGACGTGTCGTCCACCCAGTTCGCCCACATTGAGATTCCGGTTCATGCTTTCGGGTTCGGGCAGAACGCCGGCTACTGCCAGGAGCCGGCTGAGGACGCCTTTGCCAAATGCGTCGGGGGTTTACTAAGCGTCGTCGTTCAGGACTTGAGAGTTCAGCTCGGCTTCTCATCCGGCTCGGCTCCCGCTGAGATAACCGCCATTTATTCCTGCAACGGACGGCCCACCGTGCACGGCTCGTCTTCGATTCGGCTCGGAGATTTGTACGCCGAGGAAGAGAGGGAGTTGCTCGTTGAGCTTCGTATCCCTACATCGGCTGCTGGGACCCACCATGTGATGTCAGTTCGGTGCTTATACAAAGACCCGGCGACGCAAGAAGTTGTCTACGGCAAGGAGCAGGGACTAGTAGTGCCCCTCACGCAGTCAGCCGTCCGATCGCTGTCCTTGAATCCGAAGATCCAACGGTTGAGAAGACTTTTCATGACTACGCGCGCTGTAGCGGAGTCACGGAGGCTGGTTGAGCACAATGACTTCCAAAGTGCACATCACTTGCTAGCATCGACTCGAGCTCTGCTCATGCAGTCCGGCTTGTCTTCGGCTGATGAGTACGTTCGGACATTGGAGGCTCAGCTGGCGGAGTTGCATTGGAAGAGGCAGAATCAGCTGGAGGAGCAGCAGCAGCAGATGATGATAATGCAGCGGCGGAGAGTAAGCGAGAGAGAAATGGTGGCGTCGGCGGCTGTCGATGAAAACGGAGAGCCGCTGACGCCGACTTCGGCGTGGAGAGCCGCGGAGAAGCTGGCTAAGGTAGCTATGATGAAGAAGTCGTTGAATAGGGTCAGTGACTTGCACGGCTTTGAAAATGCTAGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA_2 zf-RING_11 zf-RING_2

Protein Analysis

754

Amino Acids

82.62

Weight (kDa)

9.01

Isoelectric Point (pI)

54.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 119 - 165 6e-09 Ring finger domain
zf-RING_11 PF17123 120 - 149 5.9e-09 RING-like zinc finger
zf-C3HC4 PF00097 120 - 164 2.7e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 122 - 165 2.6e-06 RING-H2 zinc finger domain
VWA PF00092 340 - 485 6.7e-15 von Willebrand factor type A domain
VWA_3 PF13768 340 - 485 2.3e-12 von Willebrand factor type A domain
VWA_2 PF13519 341 - 447 9.2e-21 von Willebrand factor type A domain
WAV3_C PF25243 613 - 687 1.1e-34 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014171)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49665
fragaria_vesca FvH4_5g33780 FvH4_5g33780
malus_domestica MD08G1207200.v1.1 MD15G1391600.v1.1
prunus_persica Prupe.1G540900_v2.0.a1
pyrus_communis pycom08g17950
rosa_chinensis RchiOBHm_Chr7g0233091
rosa_laevigata RLG00000001300
rosa_multiflora Rmu_sc0016344.1_g000006
rosa_roxburghii Rroxscaffold_3G00227690
rosa_rugosa Rorug07G0278100
rosa_samantha Rh7AG433300 Rh7BG406100 Rh7CG452700 Rh7DG421900
rosa_wichuraiana Rw7G035730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1226, 1349
AatII GACGTC 2 cut(s) 762, 1041
Acc36I ACCTGC 2 cut(s) 856, 983
AccB1I GGYRCC 1 cut(s) 955
AccI GTMKAC 1 cut(s) 1773
AccII CGCG 7 cut(s) 983, 1083, 1200, 1202, 1879, 1881, 2180
AclWI GGATC 4 cut(s) 41, 300, 818, 1840
AcoI YGGCCR 3 cut(s) 700, 904, 1192
AcsI RAATTY 2 cut(s) 344, 363
AcuI CTGAAG 2 cut(s) 185, 1052
AcyI GRCGYC 8 cut(s) 570, 747, 759, 956, 1038, 1488, 2120, 2156
AdeI CACNNNGTG 1 cut(s) 1723
AfaI GTAC 4 cut(s) 7, 459, 1655, 1997
AfiI CCNNNNNNNGG 5 cut(s) 695, 906, 1201, 1477, 1702
AflIII ACRYGT 2 cut(s) 648, 1395
AgsI TTSAA 9 cut(s) 260, 331, 343, 373, 655, 1174, 1837, 2221, 2249
AhdI GACNNNNNGTC 1 cut(s) 1399
AhlI ACTAGT 1 cut(s) 1792
AjiI CACGTC 1 cut(s) 1396
AjnI CCWGG 2 cut(s) 771, 1470
Alw21I GWGCWC 5 cut(s) 1035, 1623, 1914, 1933, 1960
Alw26I GTCTC 5 cut(s) 10, 690, 911, 1153, 1390
Alw44I GTGCAC 2 cut(s) 1619, 1929
AlwI GGATC 4 cut(s) 41, 300, 818, 1840
AlwNI CAGNNNCTG 1 cut(s) 1075
Ama87I CYCGRG 5 cut(s) 88, 106, 247, 719, 1953
AoxI GGCC 6 cut(s) 700, 904, 1192, 1257, 1269, 1610
ApaLI GTGCAC 2 cut(s) 1619, 1929
ApeKI GCWGC 8 cut(s) 887, 978, 1047, 1706, 2062, 2065, 2068, 2086
ApoI RAATTY 2 cut(s) 344, 363
ArsI GACNNNNNNTTYG 6 cut(s) 9, 41, 1382, 1414, 2146, 2178
Asp700I GAANNNNTTC 1 cut(s) 338
AspLEI GCGC 8 cut(s) 958, 983, 1085, 1200, 1202, 1212, 1881, 1883
AspS9I GGNCC 4 cut(s) 253, 994, 1611, 1713
AsuC2I CCSGG 2 cut(s) 908, 1754
AsuHPI GGTGA 3 cut(s) 580, 1036, 1265
AsuII TTCGAA 1 cut(s) 349
AsuNHI GCTAGC 1 cut(s) 1942
AvaI CYCGRG 5 cut(s) 88, 106, 247, 719, 1953
AvaII GGWCC 3 cut(s) 253, 994, 1713
BaeGI GKGCMC 4 cut(s) 1012, 1623, 1803, 1933
BalI TGGCCA 1 cut(s) 1194
BanI GGYRCC 1 cut(s) 955
BanII GRGCYC 4 cut(s) 107, 113, 248, 1960
BbsI GAAGAC 6 cut(s) 288, 389, 1293, 1623, 1867, 1974
Bbv12I GWGCWC 5 cut(s) 1035, 1623, 1914, 1933, 1960
BbvCI CCTCAGC 1 cut(s) 1482
BbvI GCAGC 8 cut(s) 874, 965, 1059, 1693, 2074, 2077, 2080, 2098
BccI CCATC 2 cut(s) 58, 1139
BceAI ACGGC 8 cut(s) 557, 911, 1200, 1625, 1639, 1792, 1802, 2257
BcgI CGANNNNNNTGC 6 cut(s) 9, 43, 199, 233, 1303, 1337
BciT130I CCWGG 2 cut(s) 773, 1472
BciVI GTATCC 1 cut(s) 1703
BcnI CCSGG 2 cut(s) 908, 1754
BcoDI GTCTC 5 cut(s) 10, 690, 911, 1153, 1390
BcuI ACTAGT 1 cut(s) 1792
BfaI CTAG 4 cut(s) 114, 1793, 1943, 2256
BfmI CTRYAG 1 cut(s) 1884
BfoI RGCGCY 1 cut(s) 959
BfuAI ACCTGC 2 cut(s) 856, 983
BfuI GTATCC 1 cut(s) 1703
BlpI GCTNAGC 1 cut(s) 2016
Bme1390I CCNGG 4 cut(s) 773, 908, 1472, 1754
Bme18I GGWCC 3 cut(s) 253, 994, 1713
BmeRI GACNNNNNGTC 1 cut(s) 1399
BmeT110I CYCGRG 5 cut(s) 88, 106, 247, 719, 1953
BmgBI CACGTC 1 cut(s) 1396
BmgT120I GGNCC 4 cut(s) 253, 994, 1611, 1713
BmiI GGNNCC 8 cut(s) 254, 948, 957, 996, 1476, 1572, 1714, 1715
BmrFI CCNGG 4 cut(s) 773, 908, 1472, 1754
BmrI ACTGGG 3 cut(s) 396, 479, 1402
BmsI GCATC 5 cut(s) 1062, 1155, 1221, 1326, 1955
BmtI GCTAGC 1 cut(s) 1946
BmuI ACTGGG 3 cut(s) 396, 479, 1402
BpiI GAAGAC 6 cut(s) 288, 389, 1293, 1623, 1867, 1974
BplI GAGNNNNNCTC 4 cut(s) 1533, 1565, 1947, 1979
BpmI CTGGAG 1 cut(s) 2075
Bpu10I CCTNAGC 2 cut(s) 1482, 2193
Bpu1102I GCTNAGC 1 cut(s) 2016
Bpu14I TTCGAA 1 cut(s) 349
BpuEI CTTGAG 2 cut(s) 299, 1558
BpuMI CCSGG 2 cut(s) 908, 1754
Bsa29I ATCGAT 1 cut(s) 1017
BsaHI GRCGYC 8 cut(s) 570, 747, 759, 956, 1038, 1488, 2120, 2156
BsaI GGTCTC 2 cut(s) 911, 1390
BsaJI CCNNGG 8 cut(s) 248, 720, 750, 772, 907, 927, 1659, 2178
BsaWI WCCGGW 4 cut(s) 140, 880, 1290, 1429
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 5 cut(s) 695, 906, 1201, 1477, 1702
Bse118I RCCGGY 6 cut(s) 952, 958, 1055, 1221, 1460, 1477
Bse1I ACTGG 6 cut(s) 13, 391, 485, 525, 1307, 1408
BseBI CCWGG 2 cut(s) 773, 1472
BseCI ATCGAT 1 cut(s) 1017
BseDI CCNNGG 8 cut(s) 248, 720, 750, 772, 907, 927, 1659, 2178
BseGI GGATG 2 cut(s) 760, 1559
BseLI CCNNNNNNNGG 5 cut(s) 695, 906, 1201, 1477, 1702
BseMII CTCAG 5 cut(s) 116, 842, 1473, 1569, 2030
BseNI ACTGG 6 cut(s) 13, 391, 485, 525, 1307, 1408
BsePI GCGCGC 2 cut(s) 1198, 1879
BseRI GAGGAG 8 cut(s) 127, 291, 743, 746, 749, 752, 755, 2072
BseSI GKGCMC 4 cut(s) 1012, 1623, 1803, 1933
BseX3I CGGCCG 2 cut(s) 700, 904
BseXI GCAGC 8 cut(s) 874, 965, 1059, 1693, 2074, 2077, 2080, 2098
BseYI CCCAGC 2 cut(s) 539, 1709
BsgI GTGCAG 1 cut(s) 884
Bsh1236I CGCG 7 cut(s) 983, 1083, 1200, 1202, 1879, 1881, 2180
Bsh1285I CGRYCG 5 cut(s) 693, 703, 907, 1017, 1826
BshFI GGCC 6 cut(s) 702, 906, 1194, 1259, 1271, 1612
BshNI GGYRCC 1 cut(s) 955
BshVI ATCGAT 1 cut(s) 1017
BsiEI CGRYCG 5 cut(s) 693, 703, 907, 1017, 1826
BsiHKAI GWGCWC 5 cut(s) 1035, 1623, 1914, 1933, 1960
BsiHKCI CYCGRG 5 cut(s) 88, 106, 247, 719, 1953
BslFI GGGAC 3 cut(s) 522, 1726, 1803
BslI CCNNNNNNNGG 5 cut(s) 695, 906, 1201, 1477, 1702
BsmAI GTCTC 5 cut(s) 10, 690, 911, 1153, 1390
BsmBI CGTCTC 1 cut(s) 10
BsmFI GGGAC 3 cut(s) 522, 1726, 1803
BsnI GGCC 6 cut(s) 702, 906, 1194, 1259, 1271, 1612
Bso31I GGTCTC 2 cut(s) 911, 1390
BsoBI CYCGRG 5 cut(s) 88, 106, 247, 719, 1953
Bsp119I TTCGAA 1 cut(s) 349
Bsp143I GATC 8 cut(s) 46, 70, 305, 823, 1014, 1120, 1823, 1845
Bsp1720I GCTNAGC 1 cut(s) 2016
BspANI GGCC 6 cut(s) 702, 906, 1194, 1259, 1271, 1612
BspCNI CTCAG 5 cut(s) 117, 841, 1474, 1570, 2029
BspDI ATCGAT 1 cut(s) 1017
BspFNI CGCG 7 cut(s) 983, 1083, 1200, 1202, 1879, 1881, 2180
BspHI TCATGA 1 cut(s) 1869
BspLI GGNNCC 8 cut(s) 254, 948, 957, 996, 1476, 1572, 1714, 1715
BspMI ACCTGC 2 cut(s) 856, 983
BspOI GCTAGC 1 cut(s) 1946
BspPI GGATC 4 cut(s) 41, 300, 818, 1840
BspT104I TTCGAA 1 cut(s) 349
BspT107I GGYRCC 1 cut(s) 955
BspTNI GGTCTC 2 cut(s) 911, 1390
BsrFI RCCGGY 6 cut(s) 952, 958, 1055, 1221, 1460, 1477
BsrI ACTGG 6 cut(s) 13, 391, 485, 525, 1307, 1408
BssAI RCCGGY 6 cut(s) 952, 958, 1055, 1221, 1460, 1477
BssECI CCNNGG 8 cut(s) 248, 720, 750, 772, 907, 927, 1659, 2178
BssHII GCGCGC 2 cut(s) 1198, 1879
BssMI GATC 8 cut(s) 46, 70, 305, 823, 1014, 1120, 1823, 1845
BssNI GRCGYC 8 cut(s) 570, 747, 759, 956, 1038, 1488, 2120, 2156
Bst2UI CCWGG 2 cut(s) 773, 1472
Bst4CI ACNGT 7 cut(s) 694, 940, 1007, 1030, 1337, 1618, 1854
Bst6I CTCTTC 7 cut(s) 332, 603, 759, 808, 1169, 1659, 2033
BstACI GRCGYC 8 cut(s) 570, 747, 759, 956, 1038, 1488, 2120, 2156
BstBI TTCGAA 1 cut(s) 349
BstDEI CTNAG 7 cut(s) 125, 828, 1482, 1517, 1578, 2016, 2193
BstDSI CCRYGG 2 cut(s) 927, 2178
BstF5I GGATG 2 cut(s) 760, 1559
BstFNI CGCG 7 cut(s) 983, 1083, 1200, 1202, 1879, 1881, 2180
BstH2I RGCGCY 1 cut(s) 959
BstHHI GCGC 8 cut(s) 958, 983, 1085, 1200, 1202, 1212, 1881, 1883
BstKTI GATC 8 cut(s) 49, 73, 308, 826, 1017, 1123, 1826, 1848
BstMAI GTCTC 5 cut(s) 10, 690, 911, 1153, 1390
BstMBI GATC 8 cut(s) 46, 70, 305, 823, 1014, 1120, 1823, 1845
BstMCI CGRYCG 5 cut(s) 693, 703, 907, 1017, 1826
BstNI CCWGG 2 cut(s) 773, 1472
BstSCI CCNGG 4 cut(s) 771, 906, 1470, 1752
BstSFI CTRYAG 1 cut(s) 1884
BstSLI GKGCMC 4 cut(s) 1012, 1623, 1803, 1933
BstUI CGCG 7 cut(s) 983, 1083, 1200, 1202, 1879, 1881, 2180
BstV1I GCAGC 8 cut(s) 874, 965, 1059, 1693, 2074, 2077, 2080, 2098
BstV2I GAAGAC 6 cut(s) 288, 389, 1293, 1623, 1867, 1974
BstX2I RGATCY 2 cut(s) 46, 1845
BstXI CCANNNNNNTGG 1 cut(s) 1093
BstYI RGATCY 2 cut(s) 46, 1845
BstZI CGGCCG 2 cut(s) 700, 904
Bsu15I ATCGAT 1 cut(s) 1017
BsuI GTATCC 1 cut(s) 1703
BsuRI GGCC 6 cut(s) 702, 906, 1194, 1259, 1271, 1612
BsuTUI ATCGAT 1 cut(s) 1017
BtgI CCRYGG 2 cut(s) 927, 2178
BtrI CACGTC 1 cut(s) 1396
BtsCI GGATG 2 cut(s) 760, 1559
BtsIMutI CAGTG 2 cut(s) 1314, 2236
BveI ACCTGC 2 cut(s) 856, 983
CaiI CAGNNNCTG 1 cut(s) 1075
CciI TCATGA 1 cut(s) 1869
CfoI GCGC 8 cut(s) 958, 983, 1085, 1200, 1202, 1212, 1881, 1883
Cfr10I RCCGGY 6 cut(s) 952, 958, 1055, 1221, 1460, 1477
Cfr13I GGNCC 4 cut(s) 253, 994, 1611, 1713
Cfr42I CCGCGG 1 cut(s) 2181
ClaI ATCGAT 1 cut(s) 1017
CseI GACGC 9 cut(s) 578, 755, 972, 1492, 1496, 1510, 1768, 2109, 2164
Csp6I GTAC 4 cut(s) 6, 458, 1654, 1996
CviQI GTAC 4 cut(s) 6, 458, 1654, 1996
DdeI CTNAG 7 cut(s) 125, 828, 1482, 1517, 1578, 2016, 2193
DinI GGCGCC 1 cut(s) 957
DpnI GATC 8 cut(s) 48, 72, 307, 825, 1016, 1122, 1825, 1847
DpnII GATC 8 cut(s) 46, 70, 305, 823, 1014, 1120, 1823, 1845
DraIII CACNNNGTG 1 cut(s) 1723
DrdI GACNNNNNNGTC 2 cut(s) 1226, 1349
DriI GACNNNNNGTC 1 cut(s) 1399
DseDI GACNNNNNNGTC 2 cut(s) 1226, 1349
EaeI YGGCCR 3 cut(s) 700, 904, 1192
EagI CGGCCG 2 cut(s) 700, 904
Eam1104I CTCTTC 7 cut(s) 332, 603, 759, 808, 1169, 1659, 2033
Eam1105I GACNNNNNGTC 1 cut(s) 1399
EarI CTCTTC 7 cut(s) 332, 603, 759, 808, 1169, 1659, 2033
EciI GGCGGA 5 cut(s) 622, 740, 939, 2039, 2107
Ecl136II GAGCTC 1 cut(s) 1958
EclXI CGGCCG 2 cut(s) 700, 904
Eco147I AGGCCT 1 cut(s) 1259
Eco24I GRGCYC 4 cut(s) 107, 113, 248, 1960
Eco31I GGTCTC 2 cut(s) 911, 1390
Eco47I GGWCC 3 cut(s) 253, 994, 1713
Eco52I CGGCCG 2 cut(s) 700, 904
Eco53kI GAGCTC 1 cut(s) 1958
Eco57I CTGAAG 2 cut(s) 185, 1052
Eco88I CYCGRG 5 cut(s) 88, 106, 247, 719, 1953
EcoICRI GAGCTC 1 cut(s) 1958
EcoO109I RGGNCCY 2 cut(s) 253, 1713
EcoRII CCWGG 2 cut(s) 771, 1470
EcoT38I GRGCYC 4 cut(s) 107, 113, 248, 1960
EgeI GGCGCC 1 cut(s) 957
EheI GGCGCC 1 cut(s) 957
Esp3I CGTCTC 1 cut(s) 10
FalI AAGNNNNNCTT 4 cut(s) 183, 215, 323, 355
FaqI GGGAC 3 cut(s) 522, 1726, 1803
FauI CCCGC 3 cut(s) 564, 1052, 1582
FblI GTMKAC 1 cut(s) 1773
FokI GGATG 2 cut(s) 767, 1546
FriOI GRGCYC 4 cut(s) 107, 113, 248, 1960
FspBI CTAG 4 cut(s) 114, 1793, 1943, 2256
GlaI GCGC 8 cut(s) 957, 982, 1084, 1199, 1201, 1211, 1880, 1882
GsaI CCCAGC 2 cut(s) 543, 1713
GsuI CTGGAG 1 cut(s) 2075
HaeII RGCGCY 1 cut(s) 959
HaeIII GGCC 6 cut(s) 702, 906, 1194, 1259, 1271, 1612
HgaI GACGC 9 cut(s) 578, 755, 972, 1492, 1496, 1510, 1768, 2109, 2164
HhaI GCGC 8 cut(s) 958, 983, 1085, 1200, 1202, 1212, 1881, 1883
Hin1I GRCGYC 8 cut(s) 570, 747, 759, 956, 1038, 1488, 2120, 2156
Hin6I GCGC 8 cut(s) 956, 981, 1083, 1198, 1200, 1210, 1879, 1881
HinP1I GCGC 8 cut(s) 956, 981, 1083, 1198, 1200, 1210, 1879, 1881
HincII GTYRAC 3 cut(s) 159, 1219, 1354
HindII GTYRAC 3 cut(s) 159, 1219, 1354
HindIII AAGCTT 1 cut(s) 1064
HphI GGTGA 3 cut(s) 580, 1036, 1265
Hpy166II GTNNAC 8 cut(s) 159, 1219, 1354, 1404, 1514, 1621, 1774, 1931
Hpy8I GTNNAC 8 cut(s) 159, 1219, 1354, 1404, 1514, 1621, 1774, 1931
HpyAV CCTTC 3 cut(s) 1008, 1261, 1270
HpyCH4III ACNGT 7 cut(s) 694, 940, 1007, 1030, 1337, 1618, 1854
HpyCH4IV ACGT 8 cut(s) 172, 510, 650, 759, 1038, 1378, 1395, 1998
HpyF3I CTNAG 7 cut(s) 125, 828, 1482, 1517, 1578, 2016, 2193
HpySE526I ACGT 8 cut(s) 172, 510, 650, 759, 1038, 1378, 1395, 1998
Hsp92I GRCGYC 8 cut(s) 570, 747, 759, 956, 1038, 1488, 2120, 2156
HspAI GCGC 8 cut(s) 956, 981, 1083, 1198, 1200, 1210, 1879, 1881
KasI GGCGCC 1 cut(s) 955
KflI GGGWCCC 1 cut(s) 1713
KroI GCCGGC 4 cut(s) 952, 958, 1460, 1477
KroNI GCCGGC 4 cut(s) 954, 960, 1462, 1479
KspI CCGCGG 1 cut(s) 2181
Kzo9I GATC 8 cut(s) 46, 70, 305, 823, 1014, 1120, 1823, 1845
LmnI GCTCC 5 cut(s) 952, 1474, 1576, 1783, 2059
Lsp1109I GCAGC 8 cut(s) 874, 965, 1059, 1693, 2074, 2077, 2080, 2098
LweI GCATC 5 cut(s) 1062, 1155, 1221, 1326, 1955
MaeI CTAG 4 cut(s) 114, 1793, 1943, 2256
MaeII ACGT 8 cut(s) 172, 510, 650, 759, 1038, 1378, 1395, 1998
MaeIII GTNAC 6 cut(s) 422, 586, 876, 1024, 1893, 2231
MalI GATC 8 cut(s) 48, 72, 307, 825, 1016, 1122, 1825, 1847
MboI GATC 8 cut(s) 46, 70, 305, 823, 1014, 1120, 1823, 1845
MflI RGATCY 2 cut(s) 46, 1845
MlsI TGGCCA 1 cut(s) 1194
MluCI AATT 3 cut(s) 344, 363, 493
MluNI TGGCCA 1 cut(s) 1194
Mly113I GGCGCC 1 cut(s) 956
MlyI GAGTC 5 cut(s) 100, 106, 544, 1901, 1945
MmeI TCCRAC 3 cut(s) 168, 713, 1873
Mox20I TGGCCA 1 cut(s) 1194
MreI CGCCGGCG 1 cut(s) 952
MroNI GCCGGC 4 cut(s) 952, 958, 1460, 1477
MroXI GAANNNNTTC 1 cut(s) 338
MscI TGGCCA 1 cut(s) 1194
MseI TTAA 1 cut(s) 2263
Msp20I TGGCCA 1 cut(s) 1194
MspA1I CMGCKG 7 cut(s) 1044, 1577, 2020, 2053, 2089, 2152, 2180
MspR9I CCNGG 4 cut(s) 773, 908, 1472, 1754
MvaI CCWGG 2 cut(s) 773, 1472
MvnI CGCG 7 cut(s) 983, 1083, 1200, 1202, 1879, 1881, 2180
NaeI GCCGGC 4 cut(s) 954, 960, 1462, 1479
NarI GGCGCC 1 cut(s) 956
NciI CCSGG 2 cut(s) 908, 1754
NdeII GATC 8 cut(s) 46, 70, 305, 823, 1014, 1120, 1823, 1845
NgoMIV GCCGGC 4 cut(s) 952, 958, 1460, 1477
NheI GCTAGC 1 cut(s) 1942
NlaIV GGNNCC 8 cut(s) 254, 948, 957, 996, 1476, 1572, 1714, 1715
NmeAIII GCCGAG 9 cut(s) 265, 775, 1089, 1094, 1124, 1186, 1530, 1547, 1684
NmuCI GTSAC 5 cut(s) 422, 586, 1024, 1893, 2231
NspV TTCGAA 1 cut(s) 349
PaeR7I CTCGAG 1 cut(s) 1953
PagI TCATGA 1 cut(s) 1869
PauI GCGCGC 2 cut(s) 1198, 1879
PceI AGGCCT 1 cut(s) 1259
PcsI WCGNNNNNNNCGW 4 cut(s) 290, 669, 1087, 1341
PdiI GCCGGC 4 cut(s) 954, 960, 1462, 1479
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 9 cut(s) 37, 548, 629, 988, 1303, 1426, 1636, 1837, 2047
Ple19I CGATCG 2 cut(s) 1017, 1826
PleI GAGTC 5 cut(s) 99, 105, 543, 1900, 1945
PluTI GGCGCC 1 cut(s) 959
PpsI GAGTC 5 cut(s) 99, 105, 543, 1900, 1945
PpuMI RGGWCCY 2 cut(s) 253, 1713
Psp124BI GAGCTC 1 cut(s) 1960
Psp5II RGGWCCY 2 cut(s) 253, 1713
Psp6I CCWGG 2 cut(s) 771, 1470
PspFI CCCAGC 2 cut(s) 539, 1709
PspGI CCWGG 2 cut(s) 771, 1470
PspN4I GGNNCC 8 cut(s) 254, 948, 957, 996, 1476, 1572, 1714, 1715
PspPI GGNCC 4 cut(s) 253, 994, 1611, 1713
PspPPI RGGWCCY 2 cut(s) 253, 1713
PspXI VCTCGAGB 1 cut(s) 1953
PsrI GAACNNNNNNTAC 2 cut(s) 1448, 1480
PstNI CAGNNNCTG 1 cut(s) 1075
PsuI RGATCY 2 cut(s) 46, 1845
PteI GCGCGC 2 cut(s) 1198, 1879
PvuI CGATCG 2 cut(s) 1017, 1826
PvuII CAGCTG 2 cut(s) 2020, 2053
RsaI GTAC 4 cut(s) 7, 459, 1655, 1997
RsaNI GTAC 4 cut(s) 6, 458, 1654, 1996
SacI GAGCTC 1 cut(s) 1960
SacII CCGCGG 1 cut(s) 2181
SaqAI TTAA 1 cut(s) 2263
Sau3AI GATC 8 cut(s) 46, 70, 305, 823, 1014, 1120, 1823, 1845
Sau96I GGNCC 4 cut(s) 253, 994, 1611, 1713
SchI GAGTC 5 cut(s) 100, 106, 544, 1901, 1945
ScrFI CCNGG 4 cut(s) 773, 908, 1472, 1754
SfaNI GCATC 5 cut(s) 1062, 1155, 1221, 1326, 1955
SfcI CTRYAG 1 cut(s) 1884
SfoI GGCGCC 1 cut(s) 957
Sfr274I CTCGAG 1 cut(s) 1953
Sfr303I CCGCGG 1 cut(s) 2181
SfuI TTCGAA 1 cut(s) 349
SgrAI CRCCGGYG 1 cut(s) 952
SgrBI CCGCGG 1 cut(s) 2181
SinI GGWCC 3 cut(s) 253, 994, 1713
SlaI CTCGAG 1 cut(s) 1953
SmlI CTYRAG 3 cut(s) 314, 1537, 1953
SmoI CTYRAG 3 cut(s) 314, 1537, 1953
SpeI ACTAGT 1 cut(s) 1792
Sse9I AATT 3 cut(s) 344, 363, 493
SseBI AGGCCT 1 cut(s) 1259
SspDI GGCGCC 1 cut(s) 955
SspMI CTAG 4 cut(s) 114, 1793, 1943, 2256
SstI GAGCTC 1 cut(s) 1960
StuI AGGCCT 1 cut(s) 1259
StyD4I CCNGG 4 cut(s) 771, 906, 1470, 1752
TaaI ACNGT 7 cut(s) 694, 940, 1007, 1030, 1337, 1618, 1854
TaiI ACGT 8 cut(s) 175, 513, 653, 762, 1041, 1381, 1398, 2001
TasI AATT 3 cut(s) 344, 363, 493
TauI GCSGC 7 cut(s) 702, 1047, 1205, 2092, 2129, 2152, 2180
TfiI GAWTC 9 cut(s) 37, 548, 629, 988, 1303, 1426, 1636, 1837, 2047
Tru1I TTAA 1 cut(s) 2263
Tru9I TTAA 1 cut(s) 2263
TscAI CASTG 2 cut(s) 1314, 2236
TseFI GTSAC 5 cut(s) 422, 586, 1024, 1893, 2231
TseI GCWGC 8 cut(s) 887, 978, 1047, 1706, 2062, 2065, 2068, 2086
Tsp45I GTSAC 5 cut(s) 422, 586, 1024, 1893, 2231
TspDTI ATGAA 6 cut(s) 422, 1280, 1424, 1858, 2151, 2222
TspGWI ACGGA 6 cut(s) 282, 475, 796, 1620, 1912, 2157
TspRI CASTG 2 cut(s) 1314, 2236
VneI GTGCAC 2 cut(s) 1619, 1929
VpaK11BI GGWCC 3 cut(s) 253, 994, 1713
XapI RAATTY 2 cut(s) 344, 363
XcmI CCANNNNNNNNNTGG 1 cut(s) 1279
XhoI CTCGAG 1 cut(s) 1953
XmiI GTMKAC 1 cut(s) 1773
XmnI GAANNNNTTC 1 cut(s) 338
XspI CTAG 4 cut(s) 114, 1793, 1943, 2256
ZraI GACGTC 2 cut(s) 760, 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.