Rh7DG258800

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0011832
Physical Location & Seq
Forward (+)
27220914 .. 27223088
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG258800.1

Sequence Viewer

Length: 1122 bp
ATGGGGTCTATAGGCAGTAGTAGTAGTTCAAAAACTTGGATACCATATATGAACAGCAGAGACTGTTCTCAAGGCTTTTGTAGTTTGTACTGTCCACAATGGTGCTACATCGTAATACCACCCCCTCCTTCCTTTGAGTTTCCTCAGGACAATTCATCTTCATCTCCAAATTTCTCTCCTCTTGTTATTGCAATCATTGGCATTTTGGTGAGTGCCTTTCTTCTTGTGAGCTACTACACCATAATGTCCAAGTACTGCGGTAACGAGGACCGAAGAAGATCAACAAGCAGAGAAGACTATGGCCAGAATGAGGAGCTGGATGGTACCCAAAATCCTTCTATCCATGAACCGTGGTATGTTGTGACTGTCGGTTTGGATGAAGGTGTGATAAAGTCATTGGGAGTTTATAAGTACAAGAATGGAGATCACTTAGTTGAAGGGACGGATTGCGCTGTCTGTCTGAGTGAGTTTGAAGAAGATGAGAGCCTGAGGCTGTTGCCCAAGTGCAACCATGCTTTTCATCTTCCATGTATTGATACATGGCTCAAGTCTCACTCGAATTGCCCTCTATGTCGCGCTAGCGTGGTTTCGACGAATGCTCCAACACATCAATTGCCTCCTGTTGTGACTGAAACTCCTCCAAGTAGTAGCAATGAAGATGAAAATGTAATCATGGGGGCACAAGTTTCAGAGACGGGTCATCCTCGAAATGTGGAGATATTGCATGGAGATGTCATTCCAAAGACACCAGTACGCGCTTTTAGTGATTTGGGTAATTCAGAAGAGAGGGATACCATAATAGAGATTGAAAGTGATGATACATTTGATCAAACAGTCAGAAGGTCAGTCTCCATGGACTATTTATGTCAGAATAATCGCATTTTAGTTGCCGATATTCTCCGCACGAATGATGAAGAAGATGAAGAACATGTGCAAGGGTGCTCACAGTTAGAGTCAGATGAGATTGCTGGTCCTTCAAAACAATCAGTAGCTGGAGGAGTTGGAACTCATAGCCGCAGAAAAGAGACTCTGAGGCCTGTTGCAATGAAGAGATCATTTTCGGGTGGGAGATTGTTTCTAGCTAGGCCTGCAAGAGCCAGGCATATAGTAATTCCACTCTAG

Protein Analysis

373

Amino Acids

41.37

Weight (kDa)

5.08

Isoelectric Point (pI)

60.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 149 - 192 1.5e-14 Ring finger domain
zf-RING_11 PF17123 149 - 178 1.2e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 150 - 191 1.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 150 - 191 3.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 153 - 192 3.2e-09 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 157 - 195 3.8e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011832)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17905 AT4G17905
fragaria_vesca FvH4_5g22340
malus_domestica MD00G1071800.v1.1 MD06G1035800.v1.1
prunus_persica Prupe.5G033900_v2.0.a1 Prupe.5G033900_v2.0.a1 Prupe.5G033900_v2.0.a1 Prupe.5G033900_v2.0.a1
pyrus_communis pycom16g24230
rosa_chinensis RchiOBHm_Chr7g0210051
rosa_laevigata RLG00000003068
rosa_multiflora Rmu_sc0004509.1_g000028
rosa_roxburghii Rroxscaffold_3G00248650
rosa_rugosa Rorug07G0118100
rosa_samantha Rh7BG246500 Rh7CG268700 Rh7DG258800
rosa_wichuraiana Rw7G021390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 408
Acc65I GGTACC 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 323
AccII CGCG 2 cut(s) 576, 756
AciI CCGC 3 cut(s) 258, 901, 1015
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 1 cut(s) 169
AfaI GTAC 5 cut(s) 89, 254, 325, 413, 753
AfiI CCNNNNNNNGG 1 cut(s) 310
AflIII ACRYGT 1 cut(s) 928
AgsI TTSAA 5 cut(s) 30, 437, 473, 809, 978
AjnI CCWGG 1 cut(s) 1097
AleI CACNNNNGTG 1 cut(s) 100
AluBI AGCT 4 cut(s) 231, 316, 992, 1082
AluI AGCT 4 cut(s) 231, 316, 992, 1082
Alw21I GWGCWC 1 cut(s) 944
Alw26I GTCTC 5 cut(s) 54, 555, 686, 853, 1019
AlwNI CAGNNNCTG 2 cut(s) 63, 992
AoxI GGCC 3 cut(s) 301, 1034, 1085
ApoI RAATTY 1 cut(s) 169
ArsI GACNNNNNNTTYG 2 cut(s) 355, 387
Asp718I GGTACC 1 cut(s) 323
AspLEI GCGC 3 cut(s) 452, 578, 758
AspS9I GGNCC 2 cut(s) 268, 971
AsuHPI GGTGA 1 cut(s) 220
AsuNHI GCTAGC 1 cut(s) 578
AvaII GGWCC 2 cut(s) 268, 971
AxyI CCTNAGG 2 cut(s) 144, 488
BaeGI GKGCMC 1 cut(s) 682
BalI TGGCCA 1 cut(s) 303
BanI GGYRCC 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 944
BccI CCATC 1 cut(s) 314
BciT130I CCWGG 1 cut(s) 1099
BciVI GTATCC 2 cut(s) 33, 784
BclI TGATCA 1 cut(s) 826
BcoDI GTCTC 5 cut(s) 54, 555, 686, 853, 1019
BfaI CTAG 4 cut(s) 579, 1079, 1083, 1120
BfmI CTRYAG 1 cut(s) 9
BfuI GTATCC 2 cut(s) 33, 784
BisI GCNGC 1 cut(s) 1015
BlsI GCNGC 1 cut(s) 1016
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 1 cut(s) 1099
Bme18I GGWCC 2 cut(s) 268, 971
BmgT120I GGNCC 2 cut(s) 268, 971
BmiI GGNNCC 1 cut(s) 325
BmrFI CCNGG 1 cut(s) 1099
BmtI GCTAGC 1 cut(s) 582
BpiI GAAGAC 1 cut(s) 300
BpmI CTGGAG 1 cut(s) 1014
BpuEI CTTGAG 2 cut(s) 54, 530
BsaJI CCNNGG 2 cut(s) 350, 852
BsaXI ACNNNNNCTCC 4 cut(s) 583, 613, 619, 649
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse1I ACTGG 1 cut(s) 749
Bse21I CCTNAGG 2 cut(s) 144, 488
Bse3DI GCAATG 2 cut(s) 658, 1050
BseBI CCWGG 1 cut(s) 1099
BseDI CCNNGG 2 cut(s) 350, 852
BseGI GGATG 3 cut(s) 325, 382, 700
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMI GCAATG 2 cut(s) 658, 1050
BseMII CTCAG 4 cut(s) 158, 452, 479, 1022
BseNI ACTGG 1 cut(s) 749
BseRI GAGGAG 4 cut(s) 168, 326, 627, 1011
BseSI GKGCMC 1 cut(s) 682
Bsh1236I CGCG 2 cut(s) 576, 756
BshFI GGCC 3 cut(s) 303, 1036, 1087
BshNI GGYRCC 1 cut(s) 323
BsiHKAI GWGCWC 1 cut(s) 944
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 1 cut(s) 310
BsmAI GTCTC 5 cut(s) 54, 555, 686, 853, 1019
BsmBI CGTCTC 1 cut(s) 686
BsmFI GGGAC 1 cut(s) 454
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 3 cut(s) 303, 1036, 1087
Bsp1286I GDGCHC 2 cut(s) 682, 944
Bsp143I GATC 4 cut(s) 278, 424, 826, 1052
Bsp19I CCATGG 1 cut(s) 852
BspACI CCGC 3 cut(s) 258, 901, 1015
BspANI GGCC 3 cut(s) 303, 1036, 1087
BspCNI CTCAG 4 cut(s) 157, 453, 480, 1023
BspFNI CGCG 2 cut(s) 576, 756
BspLI GGNNCC 1 cut(s) 325
BspOI GCTAGC 1 cut(s) 582
BspT107I GGYRCC 1 cut(s) 323
BsrDI GCAATG 2 cut(s) 658, 1050
BsrI ACTGG 1 cut(s) 749
BssECI CCNNGG 2 cut(s) 350, 852
BssMI GATC 4 cut(s) 278, 424, 826, 1052
BssT1I CCWWGG 1 cut(s) 852
Bst2UI CCWGG 1 cut(s) 1099
Bst4CI ACNGT 6 cut(s) 65, 92, 351, 367, 835, 948
Bst6I CTCTTC 2 cut(s) 777, 1043
BstC8I GCNNGC 2 cut(s) 580, 1089
BstDEI CTNAG 5 cut(s) 144, 430, 461, 488, 1031
BstDSI CCRYGG 2 cut(s) 350, 852
BstF5I GGATG 3 cut(s) 325, 382, 700
BstFNI CGCG 2 cut(s) 576, 756
BstHHI GCGC 3 cut(s) 452, 578, 758
BstKTI GATC 4 cut(s) 281, 427, 829, 1055
BstMAI GTCTC 5 cut(s) 54, 555, 686, 853, 1019
BstMBI GATC 4 cut(s) 278, 424, 826, 1052
BstMWI GCNNNNNNNGC 1 cut(s) 1088
BstNI CCWGG 1 cut(s) 1099
BstNSI RCATGY 1 cut(s) 932
BstSCI CCNGG 1 cut(s) 1097
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 1 cut(s) 682
BstUI CGCG 2 cut(s) 576, 756
BstV2I GAAGAC 1 cut(s) 300
Bsu36I CCTNAGG 2 cut(s) 144, 488
BsuI GTATCC 2 cut(s) 33, 784
BsuRI GGCC 3 cut(s) 303, 1036, 1087
BtgI CCRYGG 2 cut(s) 350, 852
BtsCI GGATG 3 cut(s) 325, 382, 700
Cac8I GCNNGC 2 cut(s) 580, 1089
CaiI CAGNNNCTG 2 cut(s) 63, 992
CfoI GCGC 3 cut(s) 452, 578, 758
Cfr13I GGNCC 2 cut(s) 268, 971
Csp6I GTAC 5 cut(s) 88, 253, 324, 412, 752
CviAII CATG 8 cut(s) 344, 512, 528, 540, 673, 725, 853, 929
CviQI GTAC 5 cut(s) 88, 253, 324, 412, 752
DdeI CTNAG 5 cut(s) 144, 430, 461, 488, 1031
DpnI GATC 4 cut(s) 280, 426, 828, 1054
DpnII GATC 4 cut(s) 278, 424, 826, 1052
EaeI YGGCCR 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 777, 1043
EarI CTCTTC 2 cut(s) 777, 1043
Eco130I CCWWGG 1 cut(s) 852
Eco147I AGGCCT 2 cut(s) 1036, 1087
Eco47I GGWCC 2 cut(s) 268, 971
Eco81I CCTNAGG 2 cut(s) 144, 488
EcoRII CCWGG 1 cut(s) 1097
EcoT14I CCWWGG 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 852
Esp3I CGTCTC 1 cut(s) 686
FaeI CATG 8 cut(s) 347, 515, 531, 543, 676, 728, 856, 932
FaqI GGGAC 1 cut(s) 454
FatI CATG 8 cut(s) 343, 511, 527, 539, 672, 724, 852, 928
FbaI TGATCA 1 cut(s) 826
Fnu4HI GCNGC 1 cut(s) 1015
FokI GGATG 3 cut(s) 332, 389, 687
Fsp4HI GCNGC 1 cut(s) 1015
FspBI CTAG 4 cut(s) 579, 1079, 1083, 1120
GlaI GCGC 3 cut(s) 451, 577, 757
GluI GCNGC 1 cut(s) 1015
GsuI CTGGAG 1 cut(s) 1014
HaeIII GGCC 3 cut(s) 303, 1036, 1087
HhaI GCGC 3 cut(s) 452, 578, 758
Hin1II CATG 8 cut(s) 347, 515, 531, 543, 676, 728, 856, 932
Hin6I GCGC 3 cut(s) 450, 576, 756
HinP1I GCGC 3 cut(s) 450, 576, 756
HinfI GANTC 2 cut(s) 953, 1027
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 1 cut(s) 95
Hpy188I TCNGA 7 cut(s) 462, 691, 781, 839, 870, 958, 1032
Hpy188III TCNNGA 1 cut(s) 146
Hpy8I GTNNAC 1 cut(s) 95
Hpy99I CGWCG 1 cut(s) 595
HpyAV CCTTC 6 cut(s) 138, 345, 374, 431, 834, 984
HpyCH4III ACNGT 6 cut(s) 65, 92, 351, 367, 835, 948
HpyCH4V TGCA 6 cut(s) 191, 507, 724, 934, 1043, 1091
HpyF10VI GCNNNNNNNGC 1 cut(s) 1088
HpyF3I CTNAG 5 cut(s) 144, 430, 461, 488, 1031
Hsp92II CATG 8 cut(s) 347, 515, 531, 543, 676, 728, 856, 932
HspAI GCGC 3 cut(s) 450, 576, 756
KpnI GGTACC 1 cut(s) 327
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 4 cut(s) 278, 424, 826, 1052
LmnI GCTCC 2 cut(s) 313, 604
MaeI CTAG 4 cut(s) 579, 1079, 1083, 1120
MaeIII GTNAC 3 cut(s) 260, 361, 625
MalI GATC 4 cut(s) 280, 426, 828, 1054
MboI GATC 4 cut(s) 278, 424, 826, 1052
MfeI CAATTG 1 cut(s) 611
MhlI GDGCHC 2 cut(s) 682, 944
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 6 cut(s) 151, 169, 559, 611, 775, 1110
MluNI TGGCCA 1 cut(s) 303
MlyI GAGTC 2 cut(s) 962, 1021
MmeI TCCRAC 2 cut(s) 626, 982
Mox20I TGGCCA 1 cut(s) 303
MscI TGGCCA 1 cut(s) 303
MslI CAYNNNNRTG 4 cut(s) 100, 206, 242, 729
Msp20I TGGCCA 1 cut(s) 303
MspR9I CCNGG 1 cut(s) 1099
MunI CAATTG 1 cut(s) 611
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 1 cut(s) 1099
MvnI CGCG 2 cut(s) 576, 756
MwoI GCNNNNNNNGC 1 cut(s) 1088
NcoI CCATGG 1 cut(s) 852
NdeII GATC 4 cut(s) 278, 424, 826, 1052
NheI GCTAGC 1 cut(s) 578
NlaIII CATG 8 cut(s) 347, 515, 531, 543, 676, 728, 856, 932
NlaIV GGNNCC 1 cut(s) 325
NmuCI GTSAC 2 cut(s) 361, 625
NspI RCATGY 1 cut(s) 932
OliI CACNNNNGTG 1 cut(s) 100
PceI AGGCCT 2 cut(s) 1036, 1087
PciI ACATGT 1 cut(s) 928
PctI GAATGC 1 cut(s) 601
PkrI GCNGC 1 cut(s) 1016
PleI GAGTC 2 cut(s) 961, 1021
PpsI GAGTC 2 cut(s) 961, 1021
PscI ACATGT 1 cut(s) 928
PsiI TTATAA 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 1097
PspGI CCWGG 1 cut(s) 1097
PspN4I GGNNCC 1 cut(s) 325
PspPI GGNCC 2 cut(s) 268, 971
PsrI GAACNNNNNNTAC 2 cut(s) 10, 42
PstNI CAGNNNCTG 2 cut(s) 63, 992
RsaI GTAC 5 cut(s) 89, 254, 325, 413, 753
RsaNI GTAC 5 cut(s) 88, 253, 324, 412, 752
RseI CAYNNNNRTG 4 cut(s) 100, 206, 242, 729
SatI GCNGC 1 cut(s) 1015
Sau3AI GATC 4 cut(s) 278, 424, 826, 1052
Sau96I GGNCC 2 cut(s) 268, 971
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 2 cut(s) 962, 1021
ScrFI CCNGG 1 cut(s) 1099
SduI GDGCHC 2 cut(s) 682, 944
SetI ASST 6 cut(s) 233, 318, 385, 845, 994, 1084
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 2 cut(s) 268, 971
SmiMI CAYNNNNRTG 4 cut(s) 100, 206, 242, 729
SmlI CTYRAG 2 cut(s) 69, 545
SmoI CTYRAG 2 cut(s) 69, 545
Sse9I AATT 6 cut(s) 151, 169, 559, 611, 775, 1110
SseBI AGGCCT 2 cut(s) 1036, 1087
SsiI CCGC 3 cut(s) 258, 901, 1015
SspMI CTAG 4 cut(s) 579, 1079, 1083, 1120
StuI AGGCCT 2 cut(s) 1036, 1087
StyD4I CCNGG 1 cut(s) 1097
StyI CCWWGG 1 cut(s) 852
TaaI ACNGT 6 cut(s) 65, 92, 351, 367, 835, 948
TaqI TCGA 3 cut(s) 557, 590, 706
TaqII GACCGA 1 cut(s) 285
TasI AATT 6 cut(s) 151, 169, 559, 611, 775, 1110
TatI WGTACW 3 cut(s) 87, 252, 411
TauI GCSGC 1 cut(s) 1017
TseFI GTSAC 2 cut(s) 361, 625
Tsp45I GTSAC 2 cut(s) 361, 625
TspGWI ACGGA 1 cut(s) 458
VpaK11BI GGWCC 2 cut(s) 268, 971
XapI RAATTY 1 cut(s) 169
XceI RCATGY 1 cut(s) 932
XspI CTAG 4 cut(s) 579, 1079, 1083, 1120
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.