Rh7DG336400

NADPH-dependent FMN reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0004397
Physical Location & Seq
Reverse (-)
43828170 .. 43829299
1130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG336400.1

Sequence Viewer

Length: 582 bp
ATGGACATGTGGAGACTATGGCACGAGAAGTACAGTGAGGAGTTAATGCAGTGGAAGGTGTTGAAGGAACACTTTGGCAGAATGGAGCAGGTGCCTGAGACACTTCCTGATGTAATATTGCAAAAGATGAAGGCCCCTCCTAAGGCAAATGATGTGCCAGAGATAAGGCCAGAACAACTTGTGGAAGCTGATGGTTTTCTCTTTGGATTTCCATCGCGTTTTGGTGTGATGTCAGCTCAATGCAAGGCCTTCTTTGATGCCAGTCATGAAATATGGGAAACACAAGCACTTGCCGGCAAACCTGCTGGAATCTTCTGGAGTACTGGATTTCATGGGGGAGGCCAGGAGCTTACTGCATTAACAGCAGTAACACAGTTGGCACATCATGGCATGATATTTGTCCCTCTTGGGTACACATTTGGAAAGGGCATGTATGAAATGAACGAGGTGAAGGGTGGCTCTTCTTATGGTGCTGGAACATTTGCAGCAGATGGATCTCGTCAACCAACAGAGCTGGAACTACAACAAGCCTTTTACCAGGGTAAATACGTGGCTGGAATTGCAAAGAAGCTTAAAAATTAA

Protein Analysis

193

Amino Acids

21.58

Weight (kDa)

6.07

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMN_red PF03358 47 - 137 9.6e-10 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 79
Acc36I ACCTGC 2 cut(s) 79, 310
AccB1I GGYRCC 1 cut(s) 91
AccII CGCG 1 cut(s) 217
AclWI GGATC 1 cut(s) 502
AfaI GTAC 3 cut(s) 32, 322, 413
AfiI CCNNNNNNNGG 1 cut(s) 142
AflIII ACRYGT 1 cut(s) 6
AgsI TTSAA 1 cut(s) 64
AjnI CCWGG 2 cut(s) 342, 537
AluBI AGCT 5 cut(s) 188, 236, 349, 514, 571
AluI AGCT 5 cut(s) 188, 236, 349, 514, 571
Alw26I GTCTC 2 cut(s) 7, 92
AlwI GGATC 1 cut(s) 502
AoxI GGCC 4 cut(s) 132, 167, 246, 340
ApeKI GCWGC 1 cut(s) 485
AspS9I GGNCC 1 cut(s) 133
AsuHPI GGTGA 1 cut(s) 460
AxyI CCTNAGG 1 cut(s) 141
BanI GGYRCC 1 cut(s) 91
BauI CACGAG 1 cut(s) 23
BbvI GCAGC 1 cut(s) 497
BccI CCATC 3 cut(s) 185, 220, 485
BciT130I CCWGG 2 cut(s) 344, 539
BcoDI GTCTC 2 cut(s) 7, 92
BfuAI ACCTGC 2 cut(s) 79, 310
BisI GCNGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 487
BmcAI AGTACT 1 cut(s) 322
Bme1390I CCNGG 2 cut(s) 344, 539
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 2 cut(s) 93, 135
BmrFI CCNGG 2 cut(s) 344, 539
BmsI GCATC 1 cut(s) 247
BpmI CTGGAG 1 cut(s) 337
BsaAI YACGTR 1 cut(s) 550
BsaBI GATNNNNATC 1 cut(s) 211
BsaJI CCNNGG 1 cut(s) 538
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 2 cut(s) 261, 328
Bse21I CCTNAGG 1 cut(s) 141
Bse8I GATNNNNATC 1 cut(s) 211
BseBI CCWGG 2 cut(s) 344, 539
BseDI CCNNGG 1 cut(s) 538
BseJI GATNNNNATC 1 cut(s) 211
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 2 cut(s) 261, 328
BseRI GAGGAG 1 cut(s) 53
BseXI GCAGC 1 cut(s) 497
Bsh1236I CGCG 1 cut(s) 217
BshFI GGCC 4 cut(s) 134, 169, 248, 342
BshNI GGYRCC 1 cut(s) 91
BsiSI CCGG 1 cut(s) 294
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 2 cut(s) 7, 92
BsmFI GGGAC 1 cut(s) 386
BsnI GGCC 4 cut(s) 134, 169, 248, 342
Bsp143I GATC 1 cut(s) 494
BspANI GGCC 4 cut(s) 134, 169, 248, 342
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 1 cut(s) 217
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 93, 135
BspMI ACCTGC 2 cut(s) 79, 310
BspPI GGATC 1 cut(s) 502
BspQI GCTCTTC 1 cut(s) 466
BspT107I GGYRCC 1 cut(s) 91
BsrFI RCCGGY 1 cut(s) 293
BsrI ACTGG 2 cut(s) 261, 328
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 1 cut(s) 538
BssMI GATC 1 cut(s) 494
BssSI CACGAG 1 cut(s) 23
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 344, 539
Bst4CI ACNGT 2 cut(s) 35, 375
Bst6I CTCTTC 1 cut(s) 466
BstBAI YACGTR 1 cut(s) 550
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 2 cut(s) 96, 141
BstFNI CGCG 1 cut(s) 217
BstKTI GATC 1 cut(s) 497
BstMAI GTCTC 2 cut(s) 7, 92
BstMBI GATC 1 cut(s) 494
BstMWI GCNNNNNNNGC 2 cut(s) 362, 560
BstNI CCWGG 2 cut(s) 344, 539
BstNSI RCATGY 2 cut(s) 10, 433
BstSCI CCNGG 2 cut(s) 342, 537
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 1 cut(s) 497
BstX2I RGATCY 1 cut(s) 494
BstYI RGATCY 1 cut(s) 494
Bsu36I CCTNAGG 1 cut(s) 141
BsuRI GGCC 4 cut(s) 134, 169, 248, 342
BtgZI GCGATG 1 cut(s) 198
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 2 cut(s) 40, 56
BveI ACCTGC 2 cut(s) 79, 310
Cac8I GCNNGC 1 cut(s) 295
CciI TCATGA 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 133
Csp6I GTAC 3 cut(s) 31, 321, 412
CviAII CATG 6 cut(s) 7, 266, 332, 386, 391, 430
CviQI GTAC 3 cut(s) 31, 321, 412
DdeI CTNAG 2 cut(s) 96, 141
DpnI GATC 1 cut(s) 496
DpnII GATC 1 cut(s) 494
Eam1104I CTCTTC 1 cut(s) 466
EarI CTCTTC 1 cut(s) 466
Eco147I AGGCCT 1 cut(s) 248
Eco81I CCTNAGG 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 133
EcoRII CCWGG 2 cut(s) 342, 537
FaeI CATG 6 cut(s) 10, 269, 335, 389, 394, 433
FalI AAGNNNNNCTT 4 cut(s) 56, 88, 236, 268
FaqI GGGAC 1 cut(s) 386
FatI CATG 6 cut(s) 6, 265, 331, 385, 390, 429
Fnu4HI GCNGC 1 cut(s) 486
Fsp4HI GCNGC 1 cut(s) 486
GluI GCNGC 1 cut(s) 486
GsuI CTGGAG 1 cut(s) 337
HaeIII GGCC 4 cut(s) 134, 169, 248, 342
HapII CCGG 1 cut(s) 294
Hin1II CATG 6 cut(s) 10, 269, 335, 389, 394, 433
HincII GTYRAC 1 cut(s) 503
HindII GTYRAC 1 cut(s) 503
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 1 cut(s) 309
HpaII CCGG 1 cut(s) 294
HphI GGTGA 1 cut(s) 460
Hpy166II GTNNAC 2 cut(s) 414, 503
Hpy188III TCNNGA 3 cut(s) 107, 266, 316
Hpy8I GTNNAC 2 cut(s) 414, 503
HpyAV CCTTC 5 cut(s) 49, 58, 124, 259, 445
HpyCH4III ACNGT 2 cut(s) 35, 375
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 6 cut(s) 49, 121, 243, 356, 485, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 560
HpyF3I CTNAG 2 cut(s) 96, 141
HpySE526I ACGT 1 cut(s) 549
Hsp92II CATG 6 cut(s) 10, 269, 335, 389, 394, 433
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Kzo9I GATC 1 cut(s) 494
LguI GCTCTTC 1 cut(s) 466
LmnI GCTCC 2 cut(s) 85, 346
Lsp1109I GCAGC 1 cut(s) 497
LweI GCATC 1 cut(s) 247
MaeII ACGT 1 cut(s) 549
MaeIII GTNAC 1 cut(s) 367
MalI GATC 1 cut(s) 496
MboI GATC 1 cut(s) 494
MboII GAAGA 2 cut(s) 304, 453
MflI RGATCY 1 cut(s) 494
MluCI AATT 2 cut(s) 558, 577
MnlI CCTC 5 cut(s) 31, 147, 332, 414, 439
MroNI GCCGGC 1 cut(s) 293
MseI TTAA 4 cut(s) 44, 359, 573, 580
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 2 cut(s) 344, 539
MvaI CCWGG 2 cut(s) 344, 539
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 2 cut(s) 362, 560
NaeI GCCGGC 1 cut(s) 295
NdeII GATC 1 cut(s) 494
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 6 cut(s) 10, 269, 335, 389, 394, 433
NlaIV GGNNCC 2 cut(s) 93, 135
NspI RCATGY 2 cut(s) 10, 433
PagI TCATGA 1 cut(s) 265
PaqCI CACCTGC 1 cut(s) 79
PceI AGGCCT 1 cut(s) 248
PciI ACATGT 1 cut(s) 6
PciSI GCTCTTC 1 cut(s) 466
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 309
PkrI GCNGC 1 cut(s) 487
Ppu21I YACGTR 1 cut(s) 550
PscI ACATGT 1 cut(s) 6
Psp6I CCWGG 2 cut(s) 342, 537
PspGI CCWGG 2 cut(s) 342, 537
PspN4I GGNNCC 2 cut(s) 93, 135
PspPI GGNCC 1 cut(s) 133
PsuI RGATCY 1 cut(s) 494
RsaI GTAC 3 cut(s) 32, 322, 413
RsaNI GTAC 3 cut(s) 31, 321, 412
SapI GCTCTTC 1 cut(s) 466
SaqAI TTAA 4 cut(s) 44, 359, 573, 580
SatI GCNGC 1 cut(s) 486
Sau3AI GATC 1 cut(s) 494
Sau96I GGNCC 1 cut(s) 133
ScaI AGTACT 1 cut(s) 322
ScrFI CCNGG 2 cut(s) 344, 539
SfaNI GCATC 1 cut(s) 247
Sse9I AATT 2 cut(s) 558, 577
SseBI AGGCCT 1 cut(s) 248
SspI AATATT 1 cut(s) 117
StuI AGGCCT 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 342, 537
TaaI ACNGT 2 cut(s) 35, 375
TaiI ACGT 1 cut(s) 552
TasI AATT 2 cut(s) 558, 577
TatI WGTACW 2 cut(s) 30, 320
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 4 cut(s) 44, 359, 573, 580
Tru9I TTAA 4 cut(s) 44, 359, 573, 580
TscAI CASTG 2 cut(s) 40, 56
TseI GCWGC 1 cut(s) 485
TspDTI ATGAA 5 cut(s) 143, 282, 320, 450, 455
TspRI CASTG 2 cut(s) 40, 56
XceI RCATGY 2 cut(s) 10, 433
ZrmI AGTACT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.