Rw2G043240

NADPH-dependent FMN reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004397
Physical Location & Seq
Forward (+)
69612354 .. 69613650
1297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G043240.1

Sequence Viewer

Length: 567 bp
ATGGCAACTACCAAGATATACATAGTGTACTACTCTTTACATGGACATGTGGAGACTATGGCACGAGAAGTACAGCGAGGAGTTAATGCAGTGGAAGGTGTTGAAGGAACACTTTGGCAGGTAATTGATCTCATTGATTTTGATTGCAATAGGCCAGAACAACTTGTGGAAGCTGATGGTTTTCTCTTTGGATTTCCATCGCGTTTTGGTGTGATGTCAGCTCAATGCAAGGCCTTCTTTGATGCCAGTCATGAAATATGGGAAACACAAGCACTTGCCGGCAAACCTGCTGGAATCTTCTGGAGTACTGGATTTCATGGGGGAGGCCAGGAGCTTACTGCATTAACAGCAGTAACACAGTTGGCACATCATGGCATGATATTTGTCCCTCTTGGGTACACATTTGGAAAGGGCATGTATGAAATGAACGAGGTGAAGGGTGGCTCTTCTTATGGTGCTGGAACATTTGCAGCAGATGGATCTCGTCAACCAACAGAGCTGGAACTACAACAAGCCTTTTACCAGGGTAAATACGTGGCTGGAATTGCAAAGAAGCTTAAAAATTAA

Protein Analysis

188

Amino Acids

20.56

Weight (kDa)

5.64

Isoelectric Point (pI)

25.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMN_red PF03358 53 - 132 2.6e-09 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 109, 295
AccII CGCG 1 cut(s) 202
AclWI GGATC 1 cut(s) 487
AfaI GTAC 4 cut(s) 29, 72, 307, 398
AflIII ACRYGT 1 cut(s) 46
AgsI TTSAA 1 cut(s) 104
AjnI CCWGG 2 cut(s) 327, 522
AluBI AGCT 5 cut(s) 173, 221, 334, 499, 556
AluI AGCT 5 cut(s) 173, 221, 334, 499, 556
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 1 cut(s) 487
AoxI GGCC 3 cut(s) 152, 231, 325
ApeKI GCWGC 1 cut(s) 470
AsuHPI GGTGA 1 cut(s) 445
BauI CACGAG 1 cut(s) 63
BbvI GCAGC 1 cut(s) 482
BccI CCATC 3 cut(s) 170, 205, 470
BciT130I CCWGG 2 cut(s) 329, 524
BcoDI GTCTC 1 cut(s) 47
BfuAI ACCTGC 2 cut(s) 109, 295
BisI GCNGC 1 cut(s) 471
BlsI GCNGC 1 cut(s) 472
BmcAI AGTACT 1 cut(s) 307
Bme1390I CCNGG 2 cut(s) 329, 524
BmrFI CCNGG 2 cut(s) 329, 524
BmsI GCATC 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 322
BsaAI YACGTR 1 cut(s) 535
BsaBI GATNNNNATC 1 cut(s) 196
BsaJI CCNNGG 1 cut(s) 523
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 2 cut(s) 246, 313
Bse8I GATNNNNATC 1 cut(s) 196
BseBI CCWGG 2 cut(s) 329, 524
BseDI CCNNGG 1 cut(s) 523
BseJI GATNNNNATC 1 cut(s) 196
BseNI ACTGG 2 cut(s) 246, 313
BseRI GAGGAG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 482
Bsh1236I CGCG 1 cut(s) 202
BshFI GGCC 3 cut(s) 154, 233, 327
BsiSI CCGG 1 cut(s) 279
BslFI GGGAC 1 cut(s) 371
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 3 cut(s) 154, 233, 327
Bsp143I GATC 2 cut(s) 127, 479
BspANI GGCC 3 cut(s) 154, 233, 327
BspFNI CGCG 1 cut(s) 202
BspHI TCATGA 1 cut(s) 250
BspMI ACCTGC 2 cut(s) 109, 295
BspPI GGATC 1 cut(s) 487
BspQI GCTCTTC 1 cut(s) 451
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 2 cut(s) 246, 313
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 1 cut(s) 523
BssMI GATC 2 cut(s) 127, 479
BssSI CACGAG 1 cut(s) 63
Bst2BI CACGAG 1 cut(s) 63
Bst2UI CCWGG 2 cut(s) 329, 524
Bst4CI ACNGT 1 cut(s) 360
Bst6I CTCTTC 1 cut(s) 451
BstBAI YACGTR 1 cut(s) 535
BstC8I GCNNGC 1 cut(s) 280
BstFNI CGCG 1 cut(s) 202
BstKTI GATC 2 cut(s) 130, 482
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 2 cut(s) 127, 479
BstMWI GCNNNNNNNGC 2 cut(s) 347, 545
BstNI CCWGG 2 cut(s) 329, 524
BstNSI RCATGY 2 cut(s) 50, 418
BstSCI CCNGG 2 cut(s) 327, 522
BstUI CGCG 1 cut(s) 202
BstV1I GCAGC 1 cut(s) 482
BstX2I RGATCY 1 cut(s) 479
BstYI RGATCY 1 cut(s) 479
BsuRI GGCC 3 cut(s) 154, 233, 327
BtgZI GCGATG 1 cut(s) 183
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 96
BveI ACCTGC 2 cut(s) 109, 295
Cac8I GCNNGC 1 cut(s) 280
CciI TCATGA 1 cut(s) 250
Cfr10I RCCGGY 1 cut(s) 278
Csp6I GTAC 4 cut(s) 28, 71, 306, 397
CviAII CATG 7 cut(s) 41, 47, 251, 317, 371, 376, 415
CviQI GTAC 4 cut(s) 28, 71, 306, 397
DpnI GATC 2 cut(s) 129, 481
DpnII GATC 2 cut(s) 127, 479
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
Eco147I AGGCCT 1 cut(s) 233
EcoRII CCWGG 2 cut(s) 327, 522
FaeI CATG 7 cut(s) 44, 50, 254, 320, 374, 379, 418
FalI AAGNNNNNCTT 4 cut(s) 96, 128, 221, 253
FaqI GGGAC 1 cut(s) 371
FatI CATG 7 cut(s) 40, 46, 250, 316, 370, 375, 414
Fnu4HI GCNGC 1 cut(s) 471
Fsp4HI GCNGC 1 cut(s) 471
GluI GCNGC 1 cut(s) 471
GsuI CTGGAG 1 cut(s) 322
HaeIII GGCC 3 cut(s) 154, 233, 327
HapII CCGG 1 cut(s) 279
Hin1II CATG 7 cut(s) 44, 50, 254, 320, 374, 379, 418
HincII GTYRAC 1 cut(s) 488
HindII GTYRAC 1 cut(s) 488
HindIII AAGCTT 1 cut(s) 554
HinfI GANTC 1 cut(s) 294
HpaII CCGG 1 cut(s) 279
HphI GGTGA 1 cut(s) 445
Hpy166II GTNNAC 3 cut(s) 28, 399, 488
Hpy188III TCNNGA 2 cut(s) 251, 301
Hpy8I GTNNAC 3 cut(s) 28, 399, 488
HpyAV CCTTC 4 cut(s) 89, 98, 244, 430
HpyCH4III ACNGT 1 cut(s) 360
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 6 cut(s) 89, 147, 228, 341, 470, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 347, 545
HpySE526I ACGT 1 cut(s) 534
Hsp92II CATG 7 cut(s) 44, 50, 254, 320, 374, 379, 418
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Kzo9I GATC 2 cut(s) 127, 479
LguI GCTCTTC 1 cut(s) 451
LmnI GCTCC 1 cut(s) 331
Lsp1109I GCAGC 1 cut(s) 482
LweI GCATC 1 cut(s) 232
MaeII ACGT 1 cut(s) 534
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 129, 481
MboI GATC 2 cut(s) 127, 479
MboII GAAGA 2 cut(s) 289, 438
MflI RGATCY 1 cut(s) 479
MluCI AATT 3 cut(s) 123, 543, 562
MnlI CCTC 4 cut(s) 71, 317, 399, 424
MroNI GCCGGC 1 cut(s) 278
MseI TTAA 4 cut(s) 84, 344, 558, 565
MslI CAYNNNNRTG 1 cut(s) 45
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 2 cut(s) 329, 524
MvaI CCWGG 2 cut(s) 329, 524
MvnI CGCG 1 cut(s) 202
MwoI GCNNNNNNNGC 2 cut(s) 347, 545
NaeI GCCGGC 1 cut(s) 280
NdeII GATC 2 cut(s) 127, 479
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 7 cut(s) 44, 50, 254, 320, 374, 379, 418
NspI RCATGY 2 cut(s) 50, 418
PagI TCATGA 1 cut(s) 250
PceI AGGCCT 1 cut(s) 233
PciI ACATGT 1 cut(s) 46
PciSI GCTCTTC 1 cut(s) 451
PdiI GCCGGC 1 cut(s) 280
PfeI GAWTC 1 cut(s) 294
PkrI GCNGC 1 cut(s) 472
Ppu21I YACGTR 1 cut(s) 535
PscI ACATGT 1 cut(s) 46
Psp6I CCWGG 2 cut(s) 327, 522
PspGI CCWGG 2 cut(s) 327, 522
PsuI RGATCY 1 cut(s) 479
RsaI GTAC 4 cut(s) 29, 72, 307, 398
RsaNI GTAC 4 cut(s) 28, 71, 306, 397
RseI CAYNNNNRTG 1 cut(s) 45
SapI GCTCTTC 1 cut(s) 451
SaqAI TTAA 4 cut(s) 84, 344, 558, 565
SatI GCNGC 1 cut(s) 471
Sau3AI GATC 2 cut(s) 127, 479
ScaI AGTACT 1 cut(s) 307
ScrFI CCNGG 2 cut(s) 329, 524
SfaNI GCATC 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 45
Sse9I AATT 3 cut(s) 123, 543, 562
SseBI AGGCCT 1 cut(s) 233
StuI AGGCCT 1 cut(s) 233
StyD4I CCNGG 2 cut(s) 327, 522
TaaI ACNGT 1 cut(s) 360
TaiI ACGT 1 cut(s) 537
TasI AATT 3 cut(s) 123, 543, 562
TatI WGTACW 3 cut(s) 27, 70, 305
TfiI GAWTC 1 cut(s) 294
Tru1I TTAA 4 cut(s) 84, 344, 558, 565
Tru9I TTAA 4 cut(s) 84, 344, 558, 565
TscAI CASTG 1 cut(s) 96
TseI GCWGC 1 cut(s) 470
TspDTI ATGAA 4 cut(s) 267, 305, 435, 440
TspRI CASTG 1 cut(s) 96
XceI RCATGY 2 cut(s) 50, 418
ZrmI AGTACT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.