Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00162

Orthogroup ID

OG0010044

Physical Location & Seq

Genomic Coordinates Reverse (-)

18932 .. 22439

Gene Structure 3508 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G004120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2151 bp

ATGGGGTCGAAAAAGAAGAACAGGAAGAACAAGAAGGAAGATAACAACATCATCACCGATCCGCGATTCGCCTCCGTCCACTGGGACCCGCGGTTCAAAAATGCTCCGAAGCACAAGGCCAAGGTCGAGATCGACGACCGCTTCAAGGCCATGTTCACCGATCCCCGCTTCGCTTCGTCCTCGGCTCCGTCAGATAAGCGCGGCAAGCTCAAGAAGAAGGCCGACCCGGGCGAGGCGCTGCGCCACTACTATCAGACCAACGAGGACCAAGAGGAGGTCAAGTACGATAAGGTGAATACCAAAGAGGATGAGCAAGAAGACGAATTGAGCGACGAAGAGGTTGAGAGTGAGAAAGCAGTAGAGAAGGTGAAATTGGAAGAGGAGGAGAGTGAGAGTGAGGAGGAAGAAGAGAAGGCGAATCTGGAAGAGGAGGAGAGCGAGAGTGAGGAATTGGAGGAGGATGATGTGGCCAGAAGCGAATCGGAGACGACGACGGATACGGGGACGGATGATGATGAAGAAGAAGATGAGGTGGTTTTTGAGGACGGTATGCCGGCAATGCAGAACGAAAATATACCGGAAATTGAGAAGGAAACTCACAGGCTTGCTGTTGTTAACCTGGATTGGAGGTATGTTAAGGCTGTTGACTTGTTTGTGGTGTTGAGGTCATTTCTTCCGAAAGGTGGAGAAATGAAGTCTGTTGCTGTGTATCCTTCGGACTTTGGAATTCAGCGTATGAAAGAGGAAGAAATTCATGGTCCTGTTGGGCTATTTGATAAGAAAGATGAGGAAGGTGATGAAGAAAGTGATGATGACGATGACGATGACGAGCTTATTAATCAGAAAATGCGTGCTTATGAGAAAAGCCGGCTGAGGTACTACTATGCTGTAGTGGAATGTGACTCAATTGCCACAGCAGATTACCTTTACAGAAATTGTGATGGAGTTGAGTTTGAAAGGTCATCAAATACTCTTGATCTAAGATTCATCCCTGATTCATTGGAAATTAAGCATCCACCCCGTGATATTGCAACGGAGGTACCTTCAAACTATGTTGGTTTGGACTTCCAAACTAGAGCTCTGCAGCAAAGTAAAATTGATATTTCTTGGGATGAGGATGAACCAGGGCGAAGGACCTTGAAACGAAAATTCACTGCTGATCAGCTTGATGAAATGGAAATGAAGGAGTTTTTGGCTTCTGATGAGAGTGAAAGTGATGAGGCCGAGGATGAGGCTGCAGATGATGCAGAGGATAAGCCTGATAAAAAGAGTAAGAAAAGAGATATGTACCGTGCTTTAATCCAGTCTGGAGATGGGGATGGTTCAGATGAGGATGGTGAAGAGGATGGCCAGGATATGGAGGTCACCTTCAATACTGGCTTGGAGGATTTAAGCAAGCGCATTCTTGAAAAGAAGGATAGGGAATCAGAAACAGTTTGGGATGCCTATCTCAGAAAAAGACGTGAGAAAAAGAAGGCTAAGAGAAACAGGTCTAATGATTCATCAGAGGATGAGAGTAGTGATTCTGATCAAGAAGCAAAAGAAGAGCCAGATGATTTCTTCATTGAAGAACCTTCAGTTAAAAAGAGTAAAAAGGAGTCTCGGAGTAAAAGTAACAAAGAAGAAAAGCAGCGCCAAGATATGGAAAAGGAAGCAGAGGCAAGTAGAAATGAGCTTGAGTTATTACTTGCTGATGACAAGGGAGCAGATAAAGGTGTAAAGGGATACAATTTGAAACGTAAAAAGGCAAAGGGAAAGAAGGGTAAGGAAGTTCCGGAGGAGAACAATATACCAACTGCTGATTTGGAAGATCCACGCTTTGCTGTCATGTTCACCTCACCCCTCTTTGCTCTGGATCCAACAGATCCGCAATTCAAAAGGAGTGCAACATATGCCCGGCAGGCAGCACTGAGACAACAGAAGGGTGACCCAGAGGAAGTGCAAGAAAGGGAAGTAAAAGTACAATCCGAGGGTTTCTCGTCAAAGATAGAGAAATCTTCATTAATCAAATCAATTAAGATGAAAGCCAAGCCCTTCGAAAGGAAGGAGGAAATTTTGCAGTTCCAAGGTAAAAAGGAAACAAAAGTTGAGCAGGATCCCCGAACCTTAGTACAACCAGTTAAGAAGAAGGCCAAAGTTGGACGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0044085 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NUC153

Protein Analysis

716

Amino Acids

82.65

Weight (kDa)

4.72

Isoelectric Point (pI)

61.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_ESF1	PF25121	199 - 345	1.3e-61	ESF1 RRM domain
NUC153	PF08159	604 - 627	2.7e-09	NUC153 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010044)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G01160
fragaria_vesca	FvH4_1g11200 FvH4_1g11200 FvH4_1g11200 FvH4_1g11200 FvH4_1g11200
malus_domestica	MD02G1124600.v1.1 MD15G1238700.v1.1
prunus_persica	Prupe.7G173800_v2.0.a1
pyrus_communis	pycom02g09730 pycom15g21210
rosa_chinensis	RchiOBHm_Chr2g0098611
rosa_laevigata	RLG00000016812
rosa_multiflora	Rmu_sc0002776.1_g000009
rosa_roxburghii	Rroxscaffold_2G00143850
rosa_rugosa	Rorug02G0073700
rosa_samantha	Rh2AG121300 Rh2BG124900 Rh2CG125900 Rh2DG127000
rosa_wichuraiana	Rw0G004120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1039
AccB1I	GGYRCC	1 cut(s)	1039
AccB7I	CCANNNNNTGG	2 cut(s)	1357, 1642
AccII	CGCG	3 cut(s)	64, 91, 201
AccIII	TCCGGA	1 cut(s)	1774
AciI	CCGC	7 cut(s)	62, 89, 91, 139, 166, 201, 1868
AclWI	GGATC	8 cut(s)	53, 155, 1805, 1850, 1859, 1863, 2090, 2103
AcoI	YGGCCR	2 cut(s)	468, 1348
AcsI	RAATTY	4 cut(s)	726, 750, 1148, 2052
AcuI	CTGAAG	1 cut(s)	1560
AdeI	CACNNNGTG	1 cut(s)	1022
AfaI	GTAC	6 cut(s)	284, 878, 1041, 1289, 1962, 2112
AfiI	CCNNNNNNNGG	8 cut(s)	81, 145, 232, 274, 683, 1357, 1642, 2040
AjiI	CACGTC	1 cut(s)	1463
AjnI	CCWGG	3 cut(s)	618, 1123, 1350
AjuI	GAANNNNNNNTTGG	4 cut(s)	356, 388, 1175, 1207
AloI	GAACNNNNNNTCC	2 cut(s)	77, 109
AluBI	AGCT	5 cut(s)	208, 832, 1079, 1165, 1675
AluI	AGCT	5 cut(s)	208, 832, 1079, 1165, 1675
Alw21I	GWGCWC	1 cut(s)	1081
Alw26I	GTCTC	3 cut(s)	479, 1605, 1906
AlwI	GGATC	8 cut(s)	53, 155, 1805, 1850, 1859, 1863, 2090, 2103
Ama87I	CYCGRG	1 cut(s)	226
Aor13HI	TCCGGA	1 cut(s)	1774
AoxI	GGCC	7 cut(s)	117, 147, 219, 468, 1221, 1348, 2130
ApeKI	GCWGC	5 cut(s)	238, 1084, 1235, 1630, 1904
ApoI	RAATTY	4 cut(s)	726, 750, 1148, 2052
AseI	ATTAAT	2 cut(s)	837, 2003
Asp700I	GAANNNNTTC	1 cut(s)	750
Asp718I	GGTACC	1 cut(s)	1039
AspLEI	GCGC	5 cut(s)	201, 238, 243, 1401, 1635
AspS9I	GGNCC	4 cut(s)	85, 265, 758, 1134
AsuC2I	CCSGG	3 cut(s)	227, 228, 1897
AsuII	TTCGAA	1 cut(s)	2037
AvaI	CYCGRG	1 cut(s)	226
AvaII	GGWCC	4 cut(s)	85, 265, 758, 1134
BaeI	ACNNNNGTAYC	2 cut(s)	1023, 1056
BalI	TGGCCA	2 cut(s)	470, 1350
BamHI	GGATCC	2 cut(s)	1855, 2095
BanI	GGYRCC	1 cut(s)	1039
BanII	GRGCYC	1 cut(s)	1081
BarI	GAAGNNNNNNTAC	2 cut(s)	1944, 1976
BbsI	GAAGAC	1 cut(s)	324
Bbv12I	GWGCWC	1 cut(s)	1081
BbvCI	CCTCAGC	1 cut(s)	872
BbvI	GCAGC	5 cut(s)	225, 1096, 1222, 1642, 1916
BccI	CCATC	5 cut(s)	935, 1307, 1313, 1328, 1340
BciT130I	CCWGG	3 cut(s)	620, 1125, 1352
BciVI	GTATCC	3 cut(s)	490, 720, 1718
BclI	TGATCA	2 cut(s)	1159, 1528
BcnI	CCSGG	3 cut(s)	227, 228, 1897
BcoDI	GTCTC	3 cut(s)	479, 1605, 1906
BfaI	CTAG	1 cut(s)	1074
BfmI	CTRYAG	3 cut(s)	888, 1082, 1236
BfoI	RGCGCY	2 cut(s)	239, 1636
BfuI	GTATCC	3 cut(s)	490, 720, 1718
BglI	GCCNNNNNGGC	1 cut(s)	1901
BisI	GCNGC	6 cut(s)	202, 239, 1085, 1236, 1631, 1905
BlsI	GCNGC	6 cut(s)	203, 240, 1086, 1237, 1632, 1906
Bme1390I	CCNGG	6 cut(s)	227, 228, 620, 1125, 1352, 1897
Bme18I	GGWCC	4 cut(s)	85, 265, 758, 1134
BmeT110I	CYCGRG	1 cut(s)	226
BmgBI	CACGTC	1 cut(s)	1463
BmgT120I	GGNCC	4 cut(s)	85, 265, 758, 1134
BmiI	GGNNCC	6 cut(s)	86, 87, 186, 1041, 1857, 2097
BmrFI	CCNGG	6 cut(s)	227, 228, 620, 1125, 1352, 1897
BmrI	ACTGGG	1 cut(s)	91
BmsI	GCATC	3 cut(s)	1021, 1234, 1432
BmuI	ACTGGG	1 cut(s)	91
BpiI	GAAGAC	1 cut(s)	324
BplI	GAGNNNNNCTC	2 cut(s)	1961, 1993
BpmI	CTGGAG	1 cut(s)	1329
Bpu10I	CCTNAGC	1 cut(s)	872
Bpu14I	TTCGAA	1 cut(s)	2037
BpuEI	CTTGAG	2 cut(s)	194, 1697
BpuMI	CCSGG	3 cut(s)	227, 228, 1897
BsaBI	GATNNNNATC	2 cut(s)	1446, 1527
BsaJI	CCNNGG	8 cut(s)	89, 120, 180, 226, 1124, 1224, 1968, 2065
BsaWI	WCCGGW	2 cut(s)	577, 1774
BsaXI	ACNNNNNCTCC	4 cut(s)	266, 296, 1597, 1627
Bsc4I	CCNNNNNNNGG	8 cut(s)	81, 145, 232, 274, 683, 1357, 1642, 2040
Bse118I	RCCGGY	2 cut(s)	553, 867
Bse1I	ACTGG	4 cut(s)	86, 1303, 1381, 2117
Bse3DI	GCAATG	1 cut(s)	564
Bse8I	GATNNNNATC	2 cut(s)	1446, 1527
BseAI	TCCGGA	1 cut(s)	1774
BseBI	CCWGG	3 cut(s)	620, 1125, 1352
BseDI	CCNNGG	8 cut(s)	89, 120, 180, 226, 1124, 1224, 1968, 2065
BseJI	GATNNNNATC	2 cut(s)	1446, 1527
BseLI	CCNNNNNNNGG	8 cut(s)	81, 145, 232, 274, 683, 1357, 1642, 2040
BseMI	GCAATG	1 cut(s)	564
BseMII	CTCAG	3 cut(s)	863, 1465, 1901
BseNI	ACTGG	4 cut(s)	86, 1303, 1381, 2117
BseRI	GAGGAG	8 cut(s)	287, 395, 398, 413, 443, 446, 470, 1793
BseXI	GCAGC	5 cut(s)	225, 1096, 1222, 1642, 1916
Bsh1236I	CGCG	3 cut(s)	64, 91, 201
Bsh1285I	CGRYCG	1 cut(s)	139
BshFI	GGCC	7 cut(s)	119, 149, 221, 470, 1223, 1350, 2132
BshNI	GGYRCC	1 cut(s)	1039
BsiEI	CGRYCG	1 cut(s)	139
BsiHKAI	GWGCWC	1 cut(s)	1081
BsiHKCI	CYCGRG	1 cut(s)	226
BsiSI	CCGG	6 cut(s)	227, 554, 578, 868, 1775, 1897
BslFI	GGGAC	2 cut(s)	98, 517
BslI	CCNNNNNNNGG	8 cut(s)	81, 145, 232, 274, 683, 1357, 1642, 2040
BsmAI	GTCTC	3 cut(s)	479, 1605, 1906
BsmBI	CGTCTC	1 cut(s)	479
BsmFI	GGGAC	2 cut(s)	98, 517
BsmI	GAATGC	1 cut(s)	1401
BsnI	GGCC	7 cut(s)	119, 149, 221, 470, 1223, 1350, 2132
BsoBI	CYCGRG	1 cut(s)	226
Bsp119I	TTCGAA	1 cut(s)	2037
Bsp1286I	GDGCHC	1 cut(s)	1081
Bsp13I	TCCGGA	1 cut(s)	1774
BspACI	CCGC	7 cut(s)	62, 89, 91, 139, 166, 201, 1868
BspANI	GGCC	7 cut(s)	119, 149, 221, 470, 1223, 1350, 2132
BspCNI	CTCAG	3 cut(s)	864, 1464, 1902
BspEI	TCCGGA	1 cut(s)	1774
BspFNI	CGCG	3 cut(s)	64, 91, 201
BspLI	GGNNCC	6 cut(s)	86, 87, 186, 1041, 1857, 2097
BspMAI	CTGCAG	2 cut(s)	1086, 1240
BspPI	GGATC	8 cut(s)	53, 155, 1805, 1850, 1859, 1863, 2090, 2103
BspQI	GCTCTTC	1 cut(s)	1539
BspT104I	TTCGAA	1 cut(s)	2037
BspT107I	GGYRCC	1 cut(s)	1039
BsrDI	GCAATG	1 cut(s)	564
BsrFI	RCCGGY	2 cut(s)	553, 867
BsrI	ACTGG	4 cut(s)	86, 1303, 1381, 2117
BssAI	RCCGGY	2 cut(s)	553, 867
BssECI	CCNNGG	8 cut(s)	89, 120, 180, 226, 1124, 1224, 1968, 2065
BssT1I	CCWWGG	2 cut(s)	120, 2065
Bst2UI	CCWGG	3 cut(s)	620, 1125, 1352
Bst4CI	ACNGT	3 cut(s)	548, 1292, 1435
Bst6I	CTCTTC	6 cut(s)	330, 372, 402, 420, 1335, 1539
BstAPI	GCANNNNNTGC	2 cut(s)	1244, 1892
BstBI	TTCGAA	1 cut(s)	2037
BstC8I	GCNNGC	7 cut(s)	206, 555, 606, 852, 869, 1397, 1902
BstDEI	CTNAG	6 cut(s)	872, 980, 1451, 1479, 1910, 2107
BstDSI	CCRYGG	1 cut(s)	89
BstEII	GGTNACC	2 cut(s)	1363, 1925
BstENI	CCTNNNNNAGG	1 cut(s)	2038
BstFNI	CGCG	3 cut(s)	64, 91, 201
BstH2I	RGCGCY	2 cut(s)	239, 1636
BstHHI	GCGC	5 cut(s)	201, 238, 243, 1401, 1635
BstMAI	GTCTC	3 cut(s)	479, 1605, 1906
BstMCI	CGRYCG	1 cut(s)	139
BstMWI	GCNNNNNNNGC	5 cut(s)	205, 559, 1244, 1892, 1901
BstNI	CCWGG	3 cut(s)	620, 1125, 1352
BstPI	GGTNACC	2 cut(s)	1363, 1925
BstSCI	CCNGG	6 cut(s)	225, 226, 618, 1123, 1350, 1895
BstSFI	CTRYAG	3 cut(s)	888, 1082, 1236
BstUI	CGCG	3 cut(s)	64, 91, 201
BstV1I	GCAGC	5 cut(s)	225, 1096, 1222, 1642, 1916
BstV2I	GAAGAC	1 cut(s)	324
BstX2I	RGATCY	4 cut(s)	1810, 1855, 1864, 2095
BstYI	RGATCY	4 cut(s)	1810, 1855, 1864, 2095
BsuI	GTATCC	3 cut(s)	490, 720, 1718
BsuRI	GGCC	7 cut(s)	119, 149, 221, 470, 1223, 1350, 2132
BtgI	CCRYGG	1 cut(s)	89
BtrI	CACGTC	1 cut(s)	1463
BtsI	GCAGTG	1 cut(s)	1152
BtsIMutI	CAGTG	3 cut(s)	79, 1152, 1907
Cac8I	GCNNGC	7 cut(s)	206, 555, 606, 852, 869, 1397, 1902
CfoI	GCGC	5 cut(s)	201, 238, 243, 1401, 1635
Cfr10I	RCCGGY	2 cut(s)	553, 867
Cfr13I	GGNCC	4 cut(s)	85, 265, 758, 1134
Cfr42I	CCGCGG	1 cut(s)	92
Cfr9I	CCCGGG	1 cut(s)	226
Csp6I	GTAC	6 cut(s)	283, 877, 1040, 1288, 1961, 2111
CviAII	CATG	3 cut(s)	151, 755, 1828
CviQI	GTAC	6 cut(s)	283, 877, 1040, 1288, 1961, 2111
DdeI	CTNAG	6 cut(s)	872, 980, 1451, 1479, 1910, 2107
DraIII	CACNNNGTG	1 cut(s)	1022
EaeI	YGGCCR	2 cut(s)	468, 1348
Eam1104I	CTCTTC	6 cut(s)	330, 372, 402, 420, 1335, 1539
EarI	CTCTTC	6 cut(s)	330, 372, 402, 420, 1335, 1539
Ecl136II	GAGCTC	1 cut(s)	1079
Eco130I	CCWWGG	2 cut(s)	120, 2065
Eco24I	GRGCYC	1 cut(s)	1081
Eco47I	GGWCC	4 cut(s)	85, 265, 758, 1134
Eco53kI	GAGCTC	1 cut(s)	1079
Eco57I	CTGAAG	1 cut(s)	1560
Eco88I	CYCGRG	1 cut(s)	226
Eco91I	GGTNACC	2 cut(s)	1363, 1925
EcoICRI	GAGCTC	1 cut(s)	1079
EcoNI	CCTNNNNNAGG	1 cut(s)	2038
EcoO109I	RGGNCCY	2 cut(s)	85, 1134
EcoO65I	GGTNACC	2 cut(s)	1363, 1925
EcoRI	GAATTC	1 cut(s)	726
EcoRII	CCWGG	3 cut(s)	618, 1123, 1350
EcoT14I	CCWWGG	2 cut(s)	120, 2065
EcoT38I	GRGCYC	1 cut(s)	1081
ErhI	CCWWGG	2 cut(s)	120, 2065
Esp3I	CGTCTC	1 cut(s)	479
FaeI	CATG	3 cut(s)	154, 758, 1831
FaqI	GGGAC	2 cut(s)	98, 517
FatI	CATG	3 cut(s)	150, 754, 1827
FauI	CCCGC	2 cut(s)	96, 173
FauNDI	CATATG	1 cut(s)	1891
FbaI	TGATCA	2 cut(s)	1159, 1528
Fnu4HI	GCNGC	6 cut(s)	202, 239, 1085, 1236, 1631, 1905
FriOI	GRGCYC	1 cut(s)	1081
Fsp4HI	GCNGC	6 cut(s)	202, 239, 1085, 1236, 1631, 1905
FspBI	CTAG	1 cut(s)	1074
GlaI	GCGC	5 cut(s)	200, 237, 242, 1400, 1634
GluI	GCNGC	6 cut(s)	202, 239, 1085, 1236, 1631, 1905
GsuI	CTGGAG	1 cut(s)	1329
HaeII	RGCGCY	2 cut(s)	239, 1636
HaeIII	GGCC	7 cut(s)	119, 149, 221, 470, 1223, 1350, 2132
HapII	CCGG	6 cut(s)	227, 554, 578, 868, 1775, 1897
HhaI	GCGC	5 cut(s)	201, 238, 243, 1401, 1635
Hin1II	CATG	3 cut(s)	154, 758, 1831
Hin6I	GCGC	5 cut(s)	199, 236, 241, 1399, 1633
HinP1I	GCGC	5 cut(s)	199, 236, 241, 1399, 1633
HincII	GTYRAC	2 cut(s)	616, 646
HindII	GTYRAC	2 cut(s)	616, 646
HpaI	GTTAAC	1 cut(s)	616
HpaII	CCGG	6 cut(s)	227, 554, 578, 868, 1775, 1897
Hpy166II	GTNNAC	5 cut(s)	79, 156, 616, 646, 1833
Hpy188III	TCNNGA	9 cut(s)	127, 211, 422, 974, 1308, 1406, 1532, 1775, 1853
Hpy8I	GTNNAC	5 cut(s)	79, 156, 616, 646, 1833
Hpy99I	CGWCG	4 cut(s)	137, 335, 493, 496
HpyCH4III	ACNGT	3 cut(s)	548, 1292, 1435
HpyCH4IV	ACGT	2 cut(s)	1462, 1738
HpyCH4V	TGCA	8 cut(s)	562, 1031, 1084, 1238, 1247, 1886, 1942, 2059
HpyF10VI	GCNNNNNNNGC	5 cut(s)	205, 559, 1244, 1892, 1901
HpyF3I	CTNAG	6 cut(s)	872, 980, 1451, 1479, 1910, 2107
HpySE526I	ACGT	2 cut(s)	1462, 1738
Hsp92II	CATG	3 cut(s)	154, 758, 1831
HspAI	GCGC	5 cut(s)	199, 236, 241, 1399, 1633
KflI	GGGWCCC	1 cut(s)	85
Kpn2I	TCCGGA	1 cut(s)	1774
KpnI	GGTACC	1 cut(s)	1043
KroI	GCCGGC	2 cut(s)	553, 867
KroNI	GCCGGC	2 cut(s)	555, 869
Ksp22I	TGATCA	2 cut(s)	1159, 1528
KspAI	GTTAAC	1 cut(s)	616
KspI	CCGCGG	1 cut(s)	92
LguI	GCTCTTC	1 cut(s)	1539
LmnI	GCTCC	3 cut(s)	109, 190, 1703
Lsp1109I	GCAGC	5 cut(s)	225, 1096, 1222, 1642, 1916
LweI	GCATC	3 cut(s)	1021, 1234, 1432
MaeI	CTAG	1 cut(s)	1074
MaeII	ACGT	2 cut(s)	1462, 1738
MaeIII	GTNAC	4 cut(s)	899, 1363, 1613, 1925
MfeI	CAATTG	1 cut(s)	906
MflI	RGATCY	4 cut(s)	1810, 1855, 1864, 2095
MhlI	GDGCHC	1 cut(s)	1081
MlsI	TGGCCA	2 cut(s)	470, 1350
MluNI	TGGCCA	2 cut(s)	470, 1350
MlyI	GAGTC	2 cut(s)	896, 1607
MmeI	TCCRAC	2 cut(s)	1883, 2119
Mox20I	TGGCCA	2 cut(s)	470, 1350
MroI	TCCGGA	1 cut(s)	1774
MroNI	GCCGGC	2 cut(s)	553, 867
MroXI	GAANNNNTTC	1 cut(s)	750
MscI	TGGCCA	2 cut(s)	470, 1350
Msp20I	TGGCCA	2 cut(s)	470, 1350
MspA1I	CMGCKG	1 cut(s)	91
MspI	CCGG	6 cut(s)	227, 554, 578, 868, 1775, 1897
MspR9I	CCNGG	6 cut(s)	227, 228, 620, 1125, 1352, 1897
MteI	GCGCNGCGC	1 cut(s)	239
MunI	CAATTG	1 cut(s)	906
Mva1269I	GAATGC	1 cut(s)	1401
MvaI	CCWGG	3 cut(s)	620, 1125, 1352
MvnI	CGCG	3 cut(s)	64, 91, 201
MwoI	GCNNNNNNNGC	5 cut(s)	205, 559, 1244, 1892, 1901
NaeI	GCCGGC	2 cut(s)	555, 869
NciI	CCSGG	3 cut(s)	227, 228, 1897
NdeI	CATATG	1 cut(s)	1891
NgoMIV	GCCGGC	2 cut(s)	553, 867
NlaIII	CATG	3 cut(s)	154, 758, 1831
NlaIV	GGNNCC	6 cut(s)	86, 87, 186, 1041, 1857, 2097
NmeAIII	GCCGAG	2 cut(s)	161, 1249
NmuCI	GTSAC	3 cut(s)	899, 1363, 1925
NspV	TTCGAA	1 cut(s)	2037
PciSI	GCTCTTC	1 cut(s)	1539
PcsI	WCGNNNNNNNCGW	3 cut(s)	132, 327, 488
PctI	GAATGC	1 cut(s)	1401
PdiI	GCCGGC	2 cut(s)	555, 869
PdmI	GAANNNNTTC	1 cut(s)	750
PfeI	GAWTC	8 cut(s)	66, 418, 479, 984, 995, 1424, 1499, 1523
PflMI	CCANNNNNTGG	2 cut(s)	1357, 1642
PkrI	GCNGC	6 cut(s)	203, 240, 1086, 1237, 1632, 1906
PleI	GAGTC	2 cut(s)	896, 1606
PpsI	GAGTC	2 cut(s)	896, 1606
PpuMI	RGGWCCY	2 cut(s)	85, 1134
PshBI	ATTAAT	2 cut(s)	837, 2003
Psp124BI	GAGCTC	1 cut(s)	1081
Psp5II	RGGWCCY	2 cut(s)	85, 1134
Psp6I	CCWGG	3 cut(s)	618, 1123, 1350
PspEI	GGTNACC	2 cut(s)	1363, 1925
PspGI	CCWGG	3 cut(s)	618, 1123, 1350
PspN4I	GGNNCC	6 cut(s)	86, 87, 186, 1041, 1857, 2097
PspPI	GGNCC	4 cut(s)	85, 265, 758, 1134
PspPPI	RGGWCCY	2 cut(s)	85, 1134
PstI	CTGCAG	2 cut(s)	1086, 1240
PsuI	RGATCY	4 cut(s)	1810, 1855, 1864, 2095
RsaI	GTAC	6 cut(s)	284, 878, 1041, 1289, 1962, 2112
RsaNI	GTAC	6 cut(s)	283, 877, 1040, 1288, 1961, 2111
SacI	GAGCTC	1 cut(s)	1081
SacII	CCGCGG	1 cut(s)	92
SapI	GCTCTTC	1 cut(s)	1539
SatI	GCNGC	6 cut(s)	202, 239, 1085, 1236, 1631, 1905
Sau96I	GGNCC	4 cut(s)	85, 265, 758, 1134
SchI	GAGTC	2 cut(s)	896, 1607
ScrFI	CCNGG	6 cut(s)	227, 228, 620, 1125, 1352, 1897
SduI	GDGCHC	1 cut(s)	1081
SfaNI	GCATC	3 cut(s)	1021, 1234, 1432
SfcI	CTRYAG	3 cut(s)	888, 1082, 1236
Sfr303I	CCGCGG	1 cut(s)	92
SfuI	TTCGAA	1 cut(s)	2037
SgrBI	CCGCGG	1 cut(s)	92
SinI	GGWCC	4 cut(s)	85, 265, 758, 1134
SmaI	CCCGGG	1 cut(s)	228
SmlI	CTYRAG	2 cut(s)	209, 1676
SmoI	CTYRAG	2 cut(s)	209, 1676
SsiI	CCGC	7 cut(s)	62, 89, 91, 139, 166, 201, 1868
SspMI	CTAG	1 cut(s)	1074
SstI	GAGCTC	1 cut(s)	1081
StyD4I	CCNGG	6 cut(s)	225, 226, 618, 1123, 1350, 1895
StyI	CCWWGG	2 cut(s)	120, 2065
TaaI	ACNGT	3 cut(s)	548, 1292, 1435
TaiI	ACGT	2 cut(s)	1465, 1741
TaqI	TCGA	4 cut(s)	8, 126, 132, 2037
TatI	WGTACW	2 cut(s)	1960, 2110
TauI	GCSGC	1 cut(s)	204
TfiI	GAWTC	8 cut(s)	66, 418, 479, 984, 995, 1424, 1499, 1523
TscAI	CASTG	3 cut(s)	86, 1159, 1914
TseFI	GTSAC	3 cut(s)	899, 1363, 1925
TseI	GCWGC	5 cut(s)	238, 1084, 1235, 1630, 1904
Tsp45I	GTSAC	3 cut(s)	899, 1363, 1925
TspGWI	ACGGA	5 cut(s)	64, 177, 509, 521, 1049
TspMI	CCCGGG	1 cut(s)	226
TspRI	CASTG	3 cut(s)	86, 1159, 1914
Van91I	CCANNNNNTGG	2 cut(s)	1357, 1642
VpaK11BI	GGWCC	4 cut(s)	85, 265, 758, 1134
VspI	ATTAAT	2 cut(s)	837, 2003
XagI	CCTNNNNNAGG	1 cut(s)	2038
XapI	RAATTY	4 cut(s)	726, 750, 1148, 2052
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1310
XmaI	CCCGGG	1 cut(s)	226
XmnI	GAANNNNTTC	1 cut(s)	750
XspI	CTAG	1 cut(s)	1074

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.