Rw1G022640

Belongs to the synaptobrevin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015867
Physical Location & Seq
Reverse (-)
48052825 .. 48055856
3032 bp
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UTR
Exon/CDS
Intron
Rw1G022640.1

Sequence Viewer

Length: 552 bp
ATGAAAATCACGGTTGAGATAGTCTGCAACACCAAATGCACTGCAATTTTAGTTAGTAAACCTGAGGAAGATGAAGAAGAAGAAGCTGCAATTTTAGTTAGTAAACCTGAGGAAGACGAAGAAGAAGAAGAGATTAGTTACCTAGTGATTTCGCGCGAACCTTTGAATGCCACAGCGTACTGCATAGTTGCAGTTGAGGCTCTTGGTCGACAAGTTCCTATTGCCTTCCTTGAGCCAATCAAGGAGGATTTTACCAATAGATATGGTGTAAGAAAAGCTGCAATGGCAGCTGAACAAGGAATTTGGGTAACAGTTCTGAAGGAGCATGTGCAATATTGTGTGGATCATCCCGATCGAGGAGATCAGCAAACTTTTTTTGATCGTGGGGAAAAGATAGAACTTCTGGTGGACAAAACAGGGAACGCCTTCGCTCACAGTTCGACTCTATACCCATTGAGGCATATTCTTTATGTTCCATTTGATTTAACTCCAGGCTGTATTGCTATGAGGTTTCAAGGTCTAATACATGGTTTCATGTGTGATTTAGACTGA

Protein Analysis

183

Amino Acids

20.68

Weight (kDa)

4.65

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Longin PF13774 49 - 96 2.7e-06 Regulated-SNARE-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015867)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04750 AT1G04750 AT1G04750
prunus_persica Prupe.2G303700_v2.0.a1 Prupe.2G303800_v2.0.a1
pyrus_communis pycom420g00800
rosa_chinensis RchiOBHm_Chr1g0355041 RchiOBHm_Chr4g0422051 RchiOBHm_Chr6g0285081
rosa_multiflora Rmu_sc0026385.1_g000001
rosa_roxburghii Rroxscaffold_4G00300740
rosa_samantha Rh1AG256900 Rh5BG278700
rosa_wichuraiana Rw1G022640 Rw2G012290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 208
AccII CGCG 2 cut(s) 154, 156
AclWI GGATC 1 cut(s) 351
AcsI RAATTY 1 cut(s) 300
AcuI CTGAAG 1 cut(s) 338
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 2 cut(s) 166, 515
AjnI CCWGG 1 cut(s) 490
AluBI AGCT 3 cut(s) 86, 278, 290
AluI AGCT 3 cut(s) 86, 278, 290
AlwI GGATC 1 cut(s) 351
ApeKI GCWGC 3 cut(s) 86, 278, 287
ApoI RAATTY 1 cut(s) 300
Asp700I GAANNNNTTC 1 cut(s) 425
AspLEI GCGC 1 cut(s) 156
AxyI CCTNAGG 2 cut(s) 63, 108
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 3 cut(s) 73, 265, 299
BciT130I CCWGG 1 cut(s) 492
BfaI CTAG 1 cut(s) 143
BisI GCNGC 3 cut(s) 87, 279, 288
BlsI GCNGC 3 cut(s) 88, 280, 289
Bme1390I CCNGG 1 cut(s) 492
BmrFI CCNGG 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 251
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse21I CCTNAGG 2 cut(s) 63, 108
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 492
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 2 cut(s) 54, 99
BseRI GAGGAG 1 cut(s) 372
BseXI GCAGC 3 cut(s) 73, 265, 299
Bsh1236I CGCG 2 cut(s) 154, 156
Bsh1285I CGRYCG 1 cut(s) 355
BsiEI CGRYCG 1 cut(s) 355
BslI CCNNNNNNNGG 1 cut(s) 356
BsmI GAATGC 1 cut(s) 172
Bsp143I GATC 4 cut(s) 343, 352, 361, 379
BspCNI CTCAG 2 cut(s) 55, 100
BspFNI CGCG 2 cut(s) 154, 156
BspPI GGATC 1 cut(s) 351
BsrDI GCAATG 1 cut(s) 288
BssMI GATC 4 cut(s) 343, 352, 361, 379
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 3 cut(s) 13, 313, 437
Bst6I CTCTTC 1 cut(s) 123
BstDEI CTNAG 2 cut(s) 63, 108
BstF5I GGATG 1 cut(s) 346
BstFNI CGCG 2 cut(s) 154, 156
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 4 cut(s) 346, 355, 364, 382
BstMBI GATC 4 cut(s) 343, 352, 361, 379
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 3 cut(s) 197, 284, 287
BstNI CCWGG 1 cut(s) 492
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 1 cut(s) 490
BstUI CGCG 2 cut(s) 154, 156
BstV1I GCAGC 3 cut(s) 73, 265, 299
BstV2I GAAGAC 1 cut(s) 120
Bsu36I CCTNAGG 2 cut(s) 63, 108
BtsCI GGATG 1 cut(s) 346
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 39
CfoI GCGC 1 cut(s) 156
Csp6I GTAC 1 cut(s) 178
CviAII CATG 3 cut(s) 326, 527, 535
CviJI RGCY 6 cut(s) 86, 200, 235, 278, 290, 495
CviKI_1 RGCY 6 cut(s) 86, 200, 235, 278, 290, 495
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 2 cut(s) 63, 108
DpnI GATC 4 cut(s) 345, 354, 363, 381
DpnII GATC 4 cut(s) 343, 352, 361, 379
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 338
Eco81I CCTNAGG 2 cut(s) 63, 108
EcoRII CCWGG 1 cut(s) 490
FaeI CATG 3 cut(s) 329, 530, 538
FaiI YATR 9 cut(s) 185, 264, 327, 448, 462, 471, 506, 528, 536
FatI CATG 3 cut(s) 325, 526, 534
FblI GTMKAC 1 cut(s) 208
Fnu4HI GCNGC 3 cut(s) 87, 279, 288
FokI GGATG 1 cut(s) 333
Fsp4HI GCNGC 3 cut(s) 87, 279, 288
FspBI CTAG 1 cut(s) 143
GlaI GCGC 1 cut(s) 155
GluI GCNGC 3 cut(s) 87, 279, 288
GsuI CTGGAG 1 cut(s) 474
HhaI GCGC 1 cut(s) 156
Hin1II CATG 3 cut(s) 329, 530, 538
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HincII GTYRAC 1 cut(s) 209
HindII GTYRAC 1 cut(s) 209
HinfI GANTC 1 cut(s) 442
Hpy166II GTNNAC 4 cut(s) 59, 104, 209, 409
Hpy188I TCNGA 1 cut(s) 318
Hpy188III TCNNGA 1 cut(s) 350
Hpy8I GTNNAC 4 cut(s) 59, 104, 209, 409
HpyAV CCTTC 3 cut(s) 235, 313, 436
HpyCH4III ACNGT 3 cut(s) 13, 313, 437
HpyCH4V TGCA 8 cut(s) 27, 39, 44, 89, 183, 191, 281, 331
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 284, 287
HpyF3I CTNAG 2 cut(s) 63, 108
Hsp92II CATG 3 cut(s) 329, 530, 538
HspAI GCGC 1 cut(s) 154
Kzo9I GATC 4 cut(s) 343, 352, 361, 379
LmnI GCTCC 1 cut(s) 322
LpnPI CCDG 6 cut(s) 75, 120, 389, 402, 477, 504
Lsp1109I GCAGC 3 cut(s) 73, 265, 299
MaeI CTAG 1 cut(s) 143
MaeIII GTNAC 2 cut(s) 137, 307
MalI GATC 4 cut(s) 345, 354, 363, 381
MboI GATC 4 cut(s) 343, 352, 361, 379
MboII GAAGA 9 cut(s) 80, 86, 89, 92, 125, 131, 134, 137, 140
MluCI AATT 3 cut(s) 45, 90, 300
MlyI GAGTC 1 cut(s) 436
MnlI CCTC 7 cut(s) 58, 103, 190, 238, 350, 450, 501
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 492
Mva1269I GAATGC 1 cut(s) 172
MvaI CCWGG 1 cut(s) 492
MvnI CGCG 2 cut(s) 154, 156
MwoI GCNNNNNNNGC 3 cut(s) 197, 284, 287
NdeII GATC 4 cut(s) 343, 352, 361, 379
NlaIII CATG 3 cut(s) 329, 530, 538
NspI RCATGY 1 cut(s) 329
PctI GAATGC 1 cut(s) 172
PdmI GAANNNNTTC 1 cut(s) 425
PkrI GCNGC 3 cut(s) 88, 280, 289
Ple19I CGATCG 1 cut(s) 355
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
Psp6I CCWGG 1 cut(s) 490
PspGI CCWGG 1 cut(s) 490
PvuI CGATCG 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SalI GTCGAC 1 cut(s) 207
SaqAI TTAA 1 cut(s) 485
SatI GCNGC 3 cut(s) 87, 279, 288
Sau3AI GATC 4 cut(s) 343, 352, 361, 379
SchI GAGTC 1 cut(s) 436
ScrFI CCNGG 1 cut(s) 492
SetI ASST 9 cut(s) 64, 88, 109, 144, 163, 280, 292, 512, 520
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 3 cut(s) 45, 90, 300
SspI AATATT 1 cut(s) 335
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 490
TaaI ACNGT 3 cut(s) 13, 313, 437
TaqI TCGA 3 cut(s) 208, 355, 440
TasI AATT 3 cut(s) 45, 90, 300
Tru1I TTAA 1 cut(s) 485
Tru9I TTAA 1 cut(s) 485
TscAI CASTG 1 cut(s) 46
TseI GCWGC 3 cut(s) 86, 278, 287
TspDTI ATGAA 3 cut(s) 17, 87, 523
TspRI CASTG 1 cut(s) 46
XapI RAATTY 1 cut(s) 300
XceI RCATGY 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.