Rw1G027580

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014957
Physical Location & Seq
Reverse (-)
55111108 .. 55114628
3521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027580.1

Sequence Viewer

Length: 1293 bp
ATGAAGTGTTTTTATTTCTACAACAAAGAGAAGAATGATGAACCAAAGACTACCAAGTCTCCTTCTGGTCATTCCACTTCCTCTTTCTTTTCACTTGATCGTGATCCAAGAAGATCAGAGTTTAATTCTCAGGATGTCTCGGAATTTAGCACAACATCCTCTGTCAAATCATTTGCAGCAATGTCTCAAAGACAGAGCAACCTAAGAGAATTCACATGTGCGGAGCTGAAAGCAGCAACAAAGAATTTTAGTCTCTCCCTCATGCTTGGAGAGGGTGGGTTTGGTGGTGTGTATAGGGGTATCATCAGGAGTGCACAAGATCCACATAAGAAGATAGATGTCGCTGTTAAACAACTGAGTAAAAGGGGATTGCAGGGCCACAAAGAGTGGGTGACTGAAGTTAATGTTTTAGGGATTGTTGAGCATCAAAATCTTGTCAAATTACTGGGCTACTGCGCTGAGGATGATGAAAGAGGGATCCAGAGGCTCCTGATATATGAATACATGCCCAACAGGAGTGTACAAGATCACTTAACAAGCCGGTTTCAGATTGCACTCCCTTGGGTCACAAGGGTCAAAATAGCACAGGATGCTGCTCGAGGATTAGCATACCTCCATGAAGAAATGGAATTTCAGATTATCTTCAGGGATTTCAAGTCTTCAAACATACTTTTGGATGAACATTGGAATGCAAAGTTATCTGACTTTGGATTAGCACGGCTGGGGCCTTCAGACGGAGTGAGCCACGTCTCTACTGCCGTTGTTGGAACAGTTGGATATGCAGCTCCTGAATACATTCAAACAGGACATCTCACGTCGAAAAGTGATGTGTGGAGTTACGGCATTTTCCTTTTTGAACTCATCACAGGAAGGCGGCCTGTGGACCGAAACCGCCCCAAGAATGAGCAAAAGCTCTTGGAATGGGTGAGAGCACATCTGTCTGCAGATTCAAAAAAGTTTCAGCTTATTATGGATCCAAGGCTCGAAGGGAAGTATTCCCTTAAGGCTGCACAAAAGCTAGCAGCTGTGGCCAACCGGTGCTTGGTTAGACAGCCGAGATCACGTCCTAAAATGAGTGAAGTACTGGAGATGATAAATAGAATTGTGGAGACGACAGCAGATATGGGAAGCCCTCAAAAACTCCCTTCAGAGAGTTTAGAGCACTCCAAAGATGAATTTTTGACCGAATCCAAAAGGGAGATTTTGAGAAGGAAGTTTGTGGATCCACTTATTGGAGAGAATGGTTGCTTGAATTTGCAAATATGGAGACCCAAGATATTGAAGACATGTTGA

Protein Analysis

430

Amino Acids

48.95

Weight (kDa)

9.23

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 87 - 364 2.8e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 88 - 363 1.1e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014957)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39110 AT2G39110
fragaria_vesca FvH4_7g18950
malus_domestica MD01G1105200.v1.1
prunus_persica Prupe.2G210500_v2.0.a1 Prupe.2G210500_v2.0.a1
pyrus_communis pycom01g13170 pycom07g16590
rosa_chinensis RchiOBHm_Chr1g0362881
rosa_laevigata RLG00000027615
rosa_multiflora Rmu_sc0001502.1_g000012
rosa_roxburghii Rroxscaffold_4G00293680
rosa_rugosa Rorug01G0302100 Rorug01G0302100
rosa_samantha Rh1BG274200
rosa_wichuraiana Rw1G027580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 55
AccB7I CCANNNNNTGG 1 cut(s) 1232
AciI CCGC 3 cut(s) 221, 874, 892
AclWI GGATC 8 cut(s) 98, 314, 472, 485, 968, 981, 1217, 1230
AcoI YGGCCR 1 cut(s) 1029
AcsI RAATTY 6 cut(s) 143, 209, 244, 629, 1175, 1252
AcuI CTGAAG 4 cut(s) 417, 628, 714, 1131
AfaI GTAC 2 cut(s) 522, 1083
AfiI CCNNNNNNNGG 3 cut(s) 734, 1042, 1232
AflII CTTAAG 1 cut(s) 1001
AflIII ACRYGT 2 cut(s) 215, 1286
AgeI ACCGGT 1 cut(s) 1035
AgsI TTSAA 7 cut(s) 655, 663, 800, 857, 951, 1252, 1282
AjiI CACGTC 3 cut(s) 748, 816, 1064
AluBI AGCT 6 cut(s) 226, 785, 913, 964, 1018, 1025
AluI AGCT 6 cut(s) 226, 785, 913, 964, 1018, 1025
Alw21I GWGCWC 3 cut(s) 316, 934, 1164
Alw26I GTCTC 7 cut(s) 63, 142, 189, 257, 754, 1103, 1261
Alw44I GTGCAC 1 cut(s) 312
AlwI GGATC 8 cut(s) 98, 314, 472, 485, 968, 981, 1217, 1230
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 1 cut(s) 597
AoxI GGCC 4 cut(s) 376, 725, 875, 1029
ApaLI GTGCAC 1 cut(s) 312
ApeKI GCWGC 6 cut(s) 176, 233, 593, 782, 1007, 1022
ApoI RAATTY 6 cut(s) 143, 209, 244, 629, 1175, 1252
AsiGI ACCGGT 1 cut(s) 1035
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 3 cut(s) 376, 725, 883
AsuHPI GGTGA 2 cut(s) 403, 937
AsuNHI GCTAGC 1 cut(s) 1018
AvaI CYCGRG 1 cut(s) 597
AvaII GGWCC 1 cut(s) 883
BaeGI GKGCMC 1 cut(s) 316
BalI TGGCCA 1 cut(s) 1031
BamHI GGATCC 3 cut(s) 477, 973, 1222
BbsI GAAGAC 2 cut(s) 651, 1289
Bbv12I GWGCWC 3 cut(s) 316, 934, 1164
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 6 cut(s) 188, 245, 580, 794, 994, 1034
BceAI ACGGC 3 cut(s) 734, 743, 856
BcoDI GTCTC 7 cut(s) 63, 142, 189, 257, 754, 1103, 1261
BfaI CTAG 1 cut(s) 1019
BfmI CTRYAG 1 cut(s) 942
BfrI CTTAAG 1 cut(s) 1001
BisI GCNGC 7 cut(s) 177, 234, 594, 783, 875, 1008, 1023
BlsI GCNGC 7 cut(s) 178, 235, 595, 784, 876, 1009, 1024
BmcAI AGTACT 1 cut(s) 1083
Bme18I GGWCC 1 cut(s) 883
BmeT110I CYCGRG 1 cut(s) 597
BmgBI CACGTC 3 cut(s) 748, 816, 1064
BmgT120I GGNCC 3 cut(s) 376, 725, 883
BmiI GGNNCC 5 cut(s) 479, 488, 726, 975, 1224
BmrI ACTGGG 1 cut(s) 455
BmsI GCATC 2 cut(s) 433, 580
BmtI GCTAGC 1 cut(s) 1022
BmuI ACTGGG 1 cut(s) 455
BpiI GAAGAC 2 cut(s) 651, 1289
BpmI CTGGAG 1 cut(s) 1106
Bpu10I CCTNAGC 1 cut(s) 459
BsaBI GATNNNNATC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 1261
BsaJI CCNNGG 2 cut(s) 560, 977
BsaWI WCCGGW 1 cut(s) 1035
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 3 cut(s) 734, 1042, 1232
Bse118I RCCGGY 2 cut(s) 540, 1035
Bse1I ACTGG 2 cut(s) 450, 1089
Bse3DI GCAATG 1 cut(s) 186
Bse8I GATNNNNATC 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 560, 977
BseGI GGATG 5 cut(s) 139, 155, 469, 595, 682
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 3 cut(s) 734, 1042, 1232
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 3 cut(s) 143, 347, 450
BseNI ACTGG 2 cut(s) 450, 1089
BseSI GKGCMC 1 cut(s) 316
BseXI GCAGC 6 cut(s) 188, 245, 580, 794, 994, 1034
BseYI CCCAGC 1 cut(s) 721
BsgI GTGCAG 1 cut(s) 993
BshFI GGCC 4 cut(s) 378, 727, 877, 1031
BshTI ACCGGT 1 cut(s) 1035
BsiHKAI GWGCWC 3 cut(s) 316, 934, 1164
BsiHKCI CYCGRG 1 cut(s) 597
BsiSI CCGG 2 cut(s) 541, 1036
BslI CCNNNNNNNGG 3 cut(s) 734, 1042, 1232
BsmAI GTCTC 7 cut(s) 63, 142, 189, 257, 754, 1103, 1261
BsmBI CGTCTC 2 cut(s) 754, 1103
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 4 cut(s) 378, 727, 877, 1031
Bso31I GGTCTC 1 cut(s) 1261
BsoBI CYCGRG 1 cut(s) 597
Bsp1286I GDGCHC 3 cut(s) 316, 934, 1164
Bsp1407I TGTACA 1 cut(s) 520
Bsp143I GATC 9 cut(s) 97, 103, 113, 319, 477, 526, 973, 1058, 1222
BspACI CCGC 3 cut(s) 221, 874, 892
BspANI GGCC 4 cut(s) 378, 727, 877, 1031
BspCNI CTCAG 3 cut(s) 142, 348, 451
BspLI GGNNCC 5 cut(s) 479, 488, 726, 975, 1224
BspMAI CTGCAG 1 cut(s) 946
BspOI GCTAGC 1 cut(s) 1022
BspPI GGATC 8 cut(s) 98, 314, 472, 485, 968, 981, 1217, 1230
BspTI CTTAAG 1 cut(s) 1001
BspTNI GGTCTC 1 cut(s) 1261
BsrDI GCAATG 1 cut(s) 186
BsrFI RCCGGY 2 cut(s) 540, 1035
BsrGI TGTACA 1 cut(s) 520
BsrI ACTGG 2 cut(s) 450, 1089
BssAI RCCGGY 2 cut(s) 540, 1035
BssECI CCNNGG 2 cut(s) 560, 977
BssMI GATC 9 cut(s) 97, 103, 113, 319, 477, 526, 973, 1058, 1222
BssT1I CCWWGG 2 cut(s) 560, 977
Bst4CI ACNGT 1 cut(s) 772
BstAFI CTTAAG 1 cut(s) 1001
BstAPI GCANNNNNTGC 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 520
BstC8I GCNNGC 1 cut(s) 1020
BstDEI CTNAG 4 cut(s) 129, 203, 356, 459
BstF5I GGATG 5 cut(s) 139, 155, 469, 595, 682
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 9 cut(s) 100, 106, 116, 322, 480, 529, 976, 1061, 1225
BstMAI GTCTC 7 cut(s) 63, 142, 189, 257, 754, 1103, 1261
BstMBI GATC 9 cut(s) 97, 103, 113, 319, 477, 526, 973, 1058, 1222
BstMWI GCNNNNNNNGC 2 cut(s) 590, 1028
BstNSI RCATGY 3 cut(s) 219, 508, 1290
BstSFI CTRYAG 1 cut(s) 942
BstSLI GKGCMC 1 cut(s) 316
BstV1I GCAGC 6 cut(s) 188, 245, 580, 794, 994, 1034
BstV2I GAAGAC 2 cut(s) 651, 1289
BstX2I RGATCY 4 cut(s) 319, 477, 973, 1222
BstYI RGATCY 4 cut(s) 319, 477, 973, 1222
BsuRI GGCC 4 cut(s) 378, 727, 877, 1031
BtrI CACGTC 3 cut(s) 748, 816, 1064
BtsCI GGATG 5 cut(s) 139, 155, 469, 595, 682
Cac8I GCNNGC 1 cut(s) 1020
CaiI CAGNNNCTG 1 cut(s) 788
CfoI GCGC 1 cut(s) 458
Cfr10I RCCGGY 2 cut(s) 540, 1035
Cfr13I GGNCC 3 cut(s) 376, 725, 883
Csp6I GTAC 2 cut(s) 521, 1082
CspAI ACCGGT 1 cut(s) 1035
CviAII CATG 5 cut(s) 216, 262, 505, 617, 1287
CviQI GTAC 2 cut(s) 521, 1082
DdeI CTNAG 4 cut(s) 129, 203, 356, 459
DpnI GATC 9 cut(s) 99, 105, 115, 321, 479, 528, 975, 1060, 1224
DpnII GATC 9 cut(s) 97, 103, 113, 319, 477, 526, 973, 1058, 1222
DrdI GACNNNNNNGTC 1 cut(s) 55
DseDI GACNNNNNNGTC 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 1029
Eco130I CCWWGG 2 cut(s) 560, 977
Eco31I GGTCTC 1 cut(s) 1261
Eco47I GGWCC 1 cut(s) 883
Eco57I CTGAAG 4 cut(s) 417, 628, 714, 1131
Eco88I CYCGRG 1 cut(s) 597
EcoO109I RGGNCCY 1 cut(s) 725
EcoRI GAATTC 1 cut(s) 209
EcoT14I CCWWGG 2 cut(s) 560, 977
ErhI CCWWGG 2 cut(s) 560, 977
Esp3I CGTCTC 2 cut(s) 754, 1103
FaeI CATG 5 cut(s) 219, 265, 508, 620, 1290
FatI CATG 5 cut(s) 215, 261, 504, 616, 1286
Fnu4HI GCNGC 7 cut(s) 177, 234, 594, 783, 875, 1008, 1023
FokI GGATG 5 cut(s) 142, 146, 476, 602, 689
Fsp4HI GCNGC 7 cut(s) 177, 234, 594, 783, 875, 1008, 1023
FspBI CTAG 1 cut(s) 1019
GlaI GCGC 1 cut(s) 457
GluI GCNGC 7 cut(s) 177, 234, 594, 783, 875, 1008, 1023
GsaI CCCAGC 1 cut(s) 725
GsuI CTGGAG 1 cut(s) 1106
HaeIII GGCC 4 cut(s) 378, 727, 877, 1031
HapII CCGG 2 cut(s) 541, 1036
HhaI GCGC 1 cut(s) 458
Hin1II CATG 5 cut(s) 219, 265, 508, 620, 1290
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HinfI GANTC 2 cut(s) 947, 1187
HpaII CCGG 2 cut(s) 541, 1036
HphI GGTGA 2 cut(s) 403, 937
Hpy166II GTNNAC 3 cut(s) 314, 521, 883
Hpy188I TCNGA 7 cut(s) 118, 142, 549, 636, 703, 733, 1150
Hpy188III TCNNGA 6 cut(s) 101, 131, 307, 481, 490, 788
Hpy8I GTNNAC 3 cut(s) 314, 521, 883
Hpy99I CGWCG 1 cut(s) 820
HpyAV CCTTC 6 cut(s) 72, 738, 864, 980, 1155, 1203
HpyCH4III ACNGT 1 cut(s) 772
HpyCH4IV ACGT 3 cut(s) 747, 815, 1063
HpyCH4V TGCA 9 cut(s) 176, 314, 373, 554, 692, 782, 944, 1010, 1258
HpyF10VI GCNNNNNNNGC 2 cut(s) 590, 1028
HpyF3I CTNAG 4 cut(s) 129, 203, 356, 459
HpySE526I ACGT 3 cut(s) 747, 815, 1063
Hsp92II CATG 5 cut(s) 219, 265, 508, 620, 1290
HspAI GCGC 1 cut(s) 456
Kzo9I GATC 9 cut(s) 97, 103, 113, 319, 477, 526, 973, 1058, 1222
LmnI GCTCC 3 cut(s) 223, 492, 790
Lsp1109I GCAGC 6 cut(s) 188, 245, 580, 794, 994, 1034
LweI GCATC 2 cut(s) 433, 580
MaeI CTAG 1 cut(s) 1019
MaeII ACGT 3 cut(s) 747, 815, 1063
MaeIII GTNAC 3 cut(s) 391, 565, 836
MalI GATC 9 cut(s) 99, 105, 115, 321, 479, 528, 975, 1060, 1224
MboI GATC 9 cut(s) 97, 103, 113, 319, 477, 526, 973, 1058, 1222
MboII GAAGA 6 cut(s) 43, 123, 343, 632, 634, 651
MflI RGATCY 4 cut(s) 319, 477, 973, 1222
MhlI GDGCHC 3 cut(s) 316, 934, 1164
MlsI TGGCCA 1 cut(s) 1031
MluCI AATT 9 cut(s) 124, 143, 209, 244, 440, 629, 1101, 1175, 1252
MluNI TGGCCA 1 cut(s) 1031
MmeI TCCRAC 2 cut(s) 745, 754
Mox20I TGGCCA 1 cut(s) 1031
MroXI GAANNNNTTC 1 cut(s) 795
MscI TGGCCA 1 cut(s) 1031
MseI TTAA 5 cut(s) 123, 348, 402, 533, 1002
MslI CAYNNNNRTG 1 cut(s) 687
Msp20I TGGCCA 1 cut(s) 1031
MspA1I CMGCKG 1 cut(s) 1025
MspCI CTTAAG 1 cut(s) 1001
MspI CCGG 2 cut(s) 541, 1036
Mva1269I GAATGC 1 cut(s) 694
MwoI GCNNNNNNNGC 2 cut(s) 590, 1028
NdeII GATC 9 cut(s) 97, 103, 113, 319, 477, 526, 973, 1058, 1222
NheI GCTAGC 1 cut(s) 1018
NlaIII CATG 5 cut(s) 219, 265, 508, 620, 1290
NlaIV GGNNCC 5 cut(s) 479, 488, 726, 975, 1224
NmeAIII GCCGAG 1 cut(s) 1080
NmuCI GTSAC 2 cut(s) 391, 565
NspI RCATGY 3 cut(s) 219, 508, 1290
PaeR7I CTCGAG 1 cut(s) 597
PciI ACATGT 2 cut(s) 215, 1286
PctI GAATGC 1 cut(s) 694
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 947, 1187
PflMI CCANNNNNTGG 1 cut(s) 1232
PinAI ACCGGT 1 cut(s) 1035
PkrI GCNGC 7 cut(s) 178, 235, 595, 784, 876, 1009, 1024
PscI ACATGT 2 cut(s) 215, 1286
PspFI CCCAGC 1 cut(s) 721
PspN4I GGNNCC 5 cut(s) 479, 488, 726, 975, 1224
PspPI GGNCC 3 cut(s) 376, 725, 883
PspXI VCTCGAGB 1 cut(s) 597
PstI CTGCAG 1 cut(s) 946
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 4 cut(s) 319, 477, 973, 1222
PvuII CAGCTG 1 cut(s) 1025
RsaI GTAC 2 cut(s) 522, 1083
RsaNI GTAC 2 cut(s) 521, 1082
RseI CAYNNNNRTG 1 cut(s) 687
SaqAI TTAA 5 cut(s) 123, 348, 402, 533, 1002
SatI GCNGC 7 cut(s) 177, 234, 594, 783, 875, 1008, 1023
Sau3AI GATC 9 cut(s) 97, 103, 113, 319, 477, 526, 973, 1058, 1222
Sau96I GGNCC 3 cut(s) 376, 725, 883
ScaI AGTACT 1 cut(s) 1083
SduI GDGCHC 3 cut(s) 316, 934, 1164
SfaNI GCATC 2 cut(s) 433, 580
SfcI CTRYAG 1 cut(s) 942
Sfr274I CTCGAG 1 cut(s) 597
SinI GGWCC 1 cut(s) 883
SlaI CTCGAG 1 cut(s) 597
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 2 cut(s) 597, 1001
SmoI CTYRAG 2 cut(s) 597, 1001
Sse9I AATT 9 cut(s) 124, 143, 209, 244, 440, 629, 1101, 1175, 1252
SsiI CCGC 3 cut(s) 221, 874, 892
SspMI CTAG 1 cut(s) 1019
StyI CCWWGG 2 cut(s) 560, 977
TaaI ACNGT 1 cut(s) 772
TaiI ACGT 3 cut(s) 750, 818, 1066
TaqI TCGA 3 cut(s) 598, 818, 984
TaqII GACCGA 2 cut(s) 900, 1199
TasI AATT 9 cut(s) 124, 143, 209, 244, 440, 629, 1101, 1175, 1252
TatI WGTACW 2 cut(s) 520, 1081
TauI GCSGC 1 cut(s) 877
TfiI GAWTC 2 cut(s) 947, 1187
Tru1I TTAA 5 cut(s) 123, 348, 402, 533, 1002
Tru9I TTAA 5 cut(s) 123, 348, 402, 533, 1002
TseFI GTSAC 2 cut(s) 391, 565
TseI GCWGC 6 cut(s) 176, 233, 593, 782, 1007, 1022
Tsp45I GTSAC 2 cut(s) 391, 565
TspDTI ATGAA 7 cut(s) 17, 54, 483, 513, 633, 693, 1188
TspGWI ACGGA 1 cut(s) 750
Van91I CCANNNNNTGG 1 cut(s) 1232
Vha464I CTTAAG 1 cut(s) 1001
VneI GTGCAC 1 cut(s) 312
VpaK11BI GGWCC 1 cut(s) 883
XapI RAATTY 6 cut(s) 143, 209, 244, 629, 1175, 1252
XceI RCATGY 3 cut(s) 219, 508, 1290
XcmI CCANNNNNNNNNTGG 1 cut(s) 1039
XhoI CTCGAG 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 1 cut(s) 1019
ZrmI AGTACT 1 cut(s) 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.