Rw1G040400

Belongs to the gluconokinase GntK GntV family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010412
Physical Location & Seq
Forward (+)
67694186 .. 67696767
2582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G040400.1

Sequence Viewer

Length: 540 bp
ATGCATTTGATTGTATTGTTTGACAGAACTATAGGTGAGATGCTGGCCAAAGAGACAAACTGTAGCTTTATTGATGCTGATGATTTCCACCCACAAGCAAACAAAGAAAAGATGAGTAAAGGGATCCCTCTTTCAGAGGAAGATCGAATTCCTTGGCTTGAAACACTTCGGAATGCCTTAAGAGAGAACTTGGTCAGTGGAAGTACTGTACTTCTTGGTTGTTCAGCTCTGCAGAAGCGGTACCGAGACATTTTAAGATCTGCAGACCCTAACTATGAACCTGGAAGTTATGCCTGTCTGGTGAAGTTCATCTTGTTAGACACTAAGGCACATGTGCTGGCTGCTCGATTAGAGAAGAGAGTGGCAGAAGGAAAGCACTTCATGTCACCTGAACTTTTGCAGTCTCAGTTAGACTTGCTTCAGATTGATGATTCTGAAGGGATACTTAAAGTTGATGCTAACCTAACTCCACAAGACATAGTAAACGCTATCCAAAGATTGTTTTTTGGTTCAAAAGTCGTCCAAGAATGTAGCCATTAG

Protein Analysis

179

Amino Acids

20.18

Weight (kDa)

5.55

Isoelectric Point (pI)

53.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SKI PF01202 10 - 120 5.9e-06 Shikimate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16790 AT2G16790 AT2G16790 AT2G16790 AT2G16790
fragaria_vesca FvH4_7g33580 FvH4_7g33580 FvH4_7g33591
malus_domestica MD01G1233600.v1.1 MD07G1305700.v1.1
prunus_persica Prupe.2G323800_v2.0.a1
pyrus_communis pycom07g27900
rosa_chinensis RchiOBHm_Chr1g0383841
rosa_laevigata RLG00000026092
rosa_multiflora Rmu_ssc0000132.1_g000001
rosa_rugosa Rorug01G0442500
rosa_samantha Rh1AG468400 Rh1BG426100 Rh1CG438800 Rh1DG458900
rosa_wichuraiana Rw1G040400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 240
AccB1I GGYRCC 1 cut(s) 240
AciI CCGC 1 cut(s) 238
AclWI GGATC 2 cut(s) 118, 131
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 1 cut(s) 147
AcuI CTGAAG 2 cut(s) 404, 456
AfaI GTAC 3 cut(s) 205, 210, 242
AflII CTTAAG 1 cut(s) 178
AflIII ACRYGT 1 cut(s) 331
AgsI TTSAA 2 cut(s) 161, 513
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 2 cut(s) 66, 227
AluI AGCT 2 cut(s) 66, 227
Alw26I GTCTC 3 cut(s) 47, 240, 408
AlwI GGATC 2 cut(s) 118, 131
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 341
ApoI RAATTY 1 cut(s) 147
Asp700I GAANNNNTTC 1 cut(s) 165
Asp718I GGTACC 1 cut(s) 240
AsuHPI GGTGA 3 cut(s) 47, 313, 378
BalI TGGCCA 1 cut(s) 47
BamHI GGATCC 1 cut(s) 123
BanI GGYRCC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 328
BciT130I CCWGG 1 cut(s) 282
BciVI GTATCC 1 cut(s) 435
BcoDI GTCTC 3 cut(s) 47, 240, 408
BfmI CTRYAG 4 cut(s) 30, 61, 230, 261
BfrI CTTAAG 1 cut(s) 178
BfuI GTATCC 1 cut(s) 435
BglII AGATCT 1 cut(s) 257
BisI GCNGC 1 cut(s) 342
BlsI GCNGC 1 cut(s) 343
BmcAI AGTACT 1 cut(s) 205
Bme1390I CCNGG 1 cut(s) 282
BmiI GGNNCC 2 cut(s) 125, 242
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 30, 64, 445
BsaJI CCNNGG 1 cut(s) 152
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 152
BseMII CTCAG 1 cut(s) 419
BseXI GCAGC 1 cut(s) 328
BshFI GGCC 1 cut(s) 47
BshNI GGYRCC 1 cut(s) 240
BsmAI GTCTC 3 cut(s) 47, 240, 408
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 1 cut(s) 47
Bsp143I GATC 3 cut(s) 123, 142, 257
BspACI CCGC 1 cut(s) 238
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 1 cut(s) 418
BspLI GGNNCC 2 cut(s) 125, 242
BspMAI CTGCAG 2 cut(s) 234, 265
BspPI GGATC 2 cut(s) 118, 131
BspT107I GGYRCC 1 cut(s) 240
BspTI CTTAAG 1 cut(s) 178
BssECI CCNNGG 1 cut(s) 152
BssMI GATC 3 cut(s) 123, 142, 257
BssT1I CCWWGG 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 62, 208
Bst6I CTCTTC 1 cut(s) 350
BstAFI CTTAAG 1 cut(s) 178
BstC8I GCNNGC 2 cut(s) 45, 339
BstDEI CTNAG 2 cut(s) 324, 405
BstKTI GATC 3 cut(s) 126, 145, 260
BstMAI GTCTC 3 cut(s) 47, 240, 408
BstMBI GATC 3 cut(s) 123, 142, 257
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 280
BstSFI CTRYAG 4 cut(s) 30, 61, 230, 261
BstV1I GCAGC 1 cut(s) 328
BstX2I RGATCY 2 cut(s) 123, 257
BstYI RGATCY 2 cut(s) 123, 257
BsuI GTATCC 1 cut(s) 435
BsuRI GGCC 1 cut(s) 47
BtsIMutI CAGTG 1 cut(s) 202
Cac8I GCNNGC 2 cut(s) 45, 339
Csp6I GTAC 3 cut(s) 204, 209, 241
CviAII CATG 2 cut(s) 332, 382
CviJI RGCY 6 cut(s) 47, 66, 157, 227, 341, 534
CviKI_1 RGCY 6 cut(s) 47, 66, 157, 227, 341, 534
CviQI GTAC 3 cut(s) 204, 209, 241
DdeI CTNAG 2 cut(s) 324, 405
DpnI GATC 3 cut(s) 125, 144, 259
DpnII GATC 3 cut(s) 123, 142, 257
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 152
Eco57I CTGAAG 2 cut(s) 404, 456
EcoRI GAATTC 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 152
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 152
FaeI CATG 2 cut(s) 335, 385
FaiI YATR 6 cut(s) 32, 276, 291, 333, 383, 479
FalI AAGNNNNNCTT 4 cut(s) 296, 328, 429, 461
FatI CATG 2 cut(s) 331, 381
Fnu4HI GCNGC 1 cut(s) 342
Fsp4HI GCNGC 1 cut(s) 342
GluI GCNGC 1 cut(s) 342
HaeIII GGCC 1 cut(s) 47
Hin1II CATG 2 cut(s) 335, 385
HinfI GANTC 1 cut(s) 431
HphI GGTGA 3 cut(s) 47, 313, 378
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 4 cut(s) 136, 171, 423, 436
Hpy8I GTNNAC 1 cut(s) 484
HpyAV CCTTC 2 cut(s) 362, 431
HpyCH4III ACNGT 2 cut(s) 62, 208
HpyCH4V TGCA 4 cut(s) 4, 232, 263, 400
HpyF3I CTNAG 2 cut(s) 324, 405
Hsp92II CATG 2 cut(s) 335, 385
KpnI GGTACC 1 cut(s) 244
Kzo9I GATC 3 cut(s) 123, 142, 257
LpnPI CCDG 7 cut(s) 29, 267, 284, 294, 307, 323, 402
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 3 cut(s) 30, 64, 445
MaeIII GTNAC 1 cut(s) 384
MalI GATC 3 cut(s) 125, 144, 259
MboI GATC 3 cut(s) 123, 142, 257
MboII GAAGA 2 cut(s) 152, 367
MflI RGATCY 2 cut(s) 123, 257
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 1 cut(s) 147
MluNI TGGCCA 1 cut(s) 47
MnlI CCTC 2 cut(s) 130, 138
Mox20I TGGCCA 1 cut(s) 47
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 165
MscI TGGCCA 1 cut(s) 47
MseI TTAA 3 cut(s) 179, 254, 447
Msp20I TGGCCA 1 cut(s) 47
MspCI CTTAAG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 178
MvaI CCWGG 1 cut(s) 282
NdeII GATC 3 cut(s) 123, 142, 257
NlaIII CATG 2 cut(s) 335, 385
NlaIV GGNNCC 2 cut(s) 125, 242
NmuCI GTSAC 1 cut(s) 384
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 335
PciI ACATGT 1 cut(s) 331
PctI GAATGC 1 cut(s) 178
PdmI GAANNNNTTC 1 cut(s) 165
PfeI GAWTC 1 cut(s) 431
PkrI GCNGC 1 cut(s) 343
PscI ACATGT 1 cut(s) 331
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 125, 242
PstI CTGCAG 2 cut(s) 234, 265
PsuI RGATCY 2 cut(s) 123, 257
RsaI GTAC 3 cut(s) 205, 210, 242
RsaNI GTAC 3 cut(s) 204, 209, 241
SaqAI TTAA 3 cut(s) 179, 254, 447
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 3 cut(s) 123, 142, 257
ScaI AGTACT 1 cut(s) 205
ScrFI CCNGG 1 cut(s) 282
SetI ASST 6 cut(s) 37, 68, 229, 283, 391, 465
SfaNI GCATC 3 cut(s) 30, 64, 445
SfcI CTRYAG 4 cut(s) 30, 61, 230, 261
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
Sse9I AATT 1 cut(s) 147
SsiI CCGC 1 cut(s) 238
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 1 cut(s) 152
TaaI ACNGT 2 cut(s) 62, 208
TaqI TCGA 2 cut(s) 145, 346
TasI AATT 1 cut(s) 147
TatI WGTACW 2 cut(s) 203, 208
TfiI GAWTC 1 cut(s) 431
Tru1I TTAA 3 cut(s) 179, 254, 447
Tru9I TTAA 3 cut(s) 179, 254, 447
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 384
TseI GCWGC 1 cut(s) 341
Tsp45I GTSAC 1 cut(s) 384
TspDTI ATGAA 3 cut(s) 291, 298, 370
TspRI CASTG 1 cut(s) 202
Vha464I CTTAAG 1 cut(s) 178
XapI RAATTY 1 cut(s) 147
XceI RCATGY 1 cut(s) 335
XmnI GAANNNNTTC 1 cut(s) 165
ZrmI AGTACT 1 cut(s) 205
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.