Rw2G010140

GDP-mannose 4,6 dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013261
Physical Location & Seq
Forward (+)
10274600 .. 10275718
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010140.1

Sequence Viewer

Length: 1119 bp
ATGGCGTCCCAGAACGACACCGCCACCGCCACCGCCGGATCCGGATCCGGATCCGGATCCAACGGCGAGGAGCGTCGCAAGGTGGCGCTGATCACCGGAATCACCGGCCAGGACGGCTCCTACCTGACGGAGTTCCTCCTCAACAAAGGCTACGACGTCCACGGCCTGATCCGACGCTCCTCCAACTTCAACACCCAGCGGATCAACCACATCTACATCGACCCCCACAACGCCCACAAGGCCCGCATGAAGCTCCACTACGCCGACCTCACCGACGCCTCCTCCCTCCGCCGCTGGCTCGACACCATCGCCCCCGACGAGGTCTACAACCTCGCCGCCCAGTCCCACGTCGCCGTTTCCTTCGAGATCCCTGACTACACCGCCGACGTCGTCGCCACCGGCGCCCTCCGCCTCCTCGAAGCCGTCCGCAACCACGTCGACGCCACCGGAAGGACCAATGTCAAGTACTACCAGGCCGGGTCCTCCGAGATGTTCGGGGCCACCCCGCCTCCGCAGTCGGAGACGACACCGTTTCATCCCCGATCCCCTTACGCCGCCTCGAAATGCGCGGCGCACTGGTACACCGTGAACTACCGCGAGGCCTACGGGCTCTACGCCTGCAATGGCATTCTGTTTAACCACGAATCGCCGAGGAGGGGAGAGAATTTCGTGACCCGGAAGATCACTCGGGCCGTGGGCCGGATCAAAATCGGGCTGCAGAGCAAGCTGTTCTTGGGGAATTTGAATGCTTCAAGGGATTGGGGATTTGCTGGTGACTATGTGGAGGCAATGTGGATGATGCTACAGCAGGAGAAGCCTGATGACTATGTTGTGGCCACCGAGGAGTCGCACACGGTGGAGGAGTTCTTGGAGGTGGCGTTTGGGTATGTGGGGATGAACTGGAAGGATCATGTTGTGCTTGACAAGAGGTACTTGAGGCCGTCGGAGGTTGACAATCTGAAAGGGGATGCAAGCAAGGCGAAGAAGATTCTCGGGTGGAAGCCCAAAGTTGGGTTTCAGCAGCTGGTGAAGATGATGGTGGATGAAGATGTCGAATTGGCAAAGAGGGAAAAGGTTCTTGTTGATGCCGGATACATGGATGCCCAGCAACAACCTTGA

Protein Analysis

372

Amino Acids

41.59

Weight (kDa)

6.65

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 29 - 279 2.6e-73 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 30 - 346 7.3e-140 GDP-mannose 4,6 dehydratase
RmlD_sub_bind PF04321 30 - 192 9.6e-06 RmlD substrate binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013261)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51160 AT5G66280
fragaria_vesca FvH4_1g12010
malus_domestica MD02G1134100.v1.1 MD15G1247100.v1.1
prunus_persica Prupe.7G166700_v2.0.a1
pyrus_communis pycom02g10570
rosa_chinensis RchiOBHm_Chr2g0099631
rosa_laevigata RLG00000016899
rosa_multiflora Rmu_sc0010438.1_g000002 Rmu_sc0028266.1_g000001
rosa_roxburghii Rroxscaffold_2G00142890
rosa_rugosa Rorug02G0081700
rosa_samantha Rh2AG131200 Rh2BG134400 Rh2CG135400 Rh2DG135800
rosa_wichuraiana Rw2G010140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 159, 390
AccB1I GGYRCC 1 cut(s) 401
AccI GTMKAC 2 cut(s) 324, 438
AccII CGCG 2 cut(s) 569, 597
AccIII TCCGGA 3 cut(s) 41, 47, 53
AcoI YGGCCR 2 cut(s) 106, 834
AcsI RAATTY 2 cut(s) 664, 739
AcyI GRCGYC 6 cut(s) 5, 156, 276, 387, 402, 441
AdeI CACNNNGTG 1 cut(s) 856
AfaI GTAC 3 cut(s) 467, 581, 932
AfiI CCNNNNNNNGG 6 cut(s) 319, 450, 656, 699, 1010, 1011
AgsI TTSAA 3 cut(s) 190, 745, 753
AjiI CACGTC 2 cut(s) 349, 436
AjnI CCWGG 2 cut(s) 108, 471
AluBI AGCT 3 cut(s) 253, 727, 1024
AluI AGCT 3 cut(s) 253, 727, 1024
Alw26I GTCTC 1 cut(s) 515
AlwNI CAGNNNCTG 1 cut(s) 1024
Ama87I CYCGRG 2 cut(s) 687, 992
Aor13HI TCCGGA 3 cut(s) 41, 47, 53
ApeKI GCWGC 2 cut(s) 715, 1021
ApoI RAATTY 2 cut(s) 664, 739
Asp700I GAANNNNTTC 1 cut(s) 1074
AspLEI GCGC 4 cut(s) 88, 404, 569, 574
AspS9I GGNCC 6 cut(s) 241, 453, 480, 498, 690, 697
AsuC2I CCSGG 2 cut(s) 478, 676
AsuHPI GGTGA 5 cut(s) 85, 94, 262, 785, 1039
AvaI CYCGRG 2 cut(s) 687, 992
AvaII GGWCC 2 cut(s) 453, 480
BalI TGGCCA 1 cut(s) 836
BamHI GGATCC 4 cut(s) 38, 44, 50, 56
BanI GGYRCC 1 cut(s) 401
BanII GRGCYC 1 cut(s) 612
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BbvI GCAGC 2 cut(s) 702, 1033
BccI CCATC 2 cut(s) 314, 1030
BceAI ACGGC 7 cut(s) 79, 130, 178, 338, 407, 677, 925
BcgI CGANNNNNNTGC 2 cut(s) 418, 452
BciT130I CCWGG 2 cut(s) 110, 473
BciVI GTATCC 1 cut(s) 1085
BclI TGATCA 1 cut(s) 90
BcnI CCSGG 2 cut(s) 478, 676
BcoDI GTCTC 1 cut(s) 515
BfmI CTRYAG 2 cut(s) 716, 803
BfoI RGCGCY 2 cut(s) 89, 405
BfuI GTATCC 1 cut(s) 1085
BglI GCCNNNNNGGC 2 cut(s) 114, 239
BisI GCNGC 6 cut(s) 292, 336, 555, 570, 716, 1022
BlsI GCNGC 6 cut(s) 293, 337, 556, 571, 717, 1023
BmcAI AGTACT 1 cut(s) 467
Bme1390I CCNGG 4 cut(s) 110, 473, 478, 676
Bme18I GGWCC 2 cut(s) 453, 480
BmeT110I CYCGRG 2 cut(s) 687, 992
BmgBI CACGTC 2 cut(s) 349, 436
BmgT120I GGNCC 6 cut(s) 241, 453, 480, 498, 690, 697
BmiI GGNNCC 8 cut(s) 40, 46, 52, 58, 118, 403, 481, 499
BmrFI CCNGG 4 cut(s) 110, 473, 478, 676
BmrI ACTGGG 1 cut(s) 334
BmsI GCATC 4 cut(s) 789, 958, 1075, 1090
BmuI ACTGGG 1 cut(s) 334
BoxI GACNNNNGTC 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 955
BpuMI CCSGG 2 cut(s) 478, 676
BsaBI GATNNNNATC 4 cut(s) 43, 49, 55, 707
BsaHI GRCGYC 6 cut(s) 5, 156, 276, 387, 402, 441
BsaJI CCNNGG 4 cut(s) 160, 650, 693, 840
BsaWI WCCGGW 5 cut(s) 41, 47, 53, 95, 446
BsaXI ACNNNNNCTCC 8 cut(s) 266, 296, 493, 523, 776, 806, 863, 893
Bsc4I CCNNNNNNNGG 6 cut(s) 319, 450, 656, 699, 1010, 1011
Bse118I RCCGGY 2 cut(s) 104, 398
Bse1I ACTGG 3 cut(s) 340, 581, 905
Bse3DI GCAATG 2 cut(s) 628, 795
Bse8I GATNNNNATC 4 cut(s) 43, 49, 55, 707
BseAI TCCGGA 3 cut(s) 41, 47, 53
BseBI CCWGG 2 cut(s) 110, 473
BseDI CCNNGG 4 cut(s) 160, 650, 693, 840
BseGI GGATG 6 cut(s) 535, 801, 900, 973, 1048, 1105
BseJI GATNNNNATC 4 cut(s) 43, 49, 55, 707
BseLI CCNNNNNNNGG 6 cut(s) 319, 450, 656, 699, 1010, 1011
BseMI GCAATG 2 cut(s) 628, 795
BseNI ACTGG 3 cut(s) 340, 581, 905
BseRI GAGGAG 8 cut(s) 83, 128, 169, 271, 404, 667, 857, 875
BseXI GCAGC 2 cut(s) 702, 1033
BseYI CCCAGC 2 cut(s) 195, 1104
Bsh1236I CGCG 2 cut(s) 569, 597
BshNI GGYRCC 1 cut(s) 401
BsiHKCI CYCGRG 2 cut(s) 687, 992
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 6 cut(s) 319, 450, 656, 699, 1010, 1011
BsmAI GTCTC 1 cut(s) 515
BsmBI CGTCTC 1 cut(s) 515
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 2 cut(s) 627, 751
BsoBI CYCGRG 2 cut(s) 687, 992
Bsp1286I GDGCHC 1 cut(s) 612
Bsp13I TCCGGA 3 cut(s) 41, 47, 53
BspEI TCCGGA 3 cut(s) 41, 47, 53
BspFNI CGCG 2 cut(s) 569, 597
BspLI GGNNCC 8 cut(s) 40, 46, 52, 58, 118, 403, 481, 499
BspMAI CTGCAG 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 401
BsrDI GCAATG 2 cut(s) 628, 795
BsrFI RCCGGY 2 cut(s) 104, 398
BsrI ACTGG 3 cut(s) 340, 581, 905
BssAI RCCGGY 2 cut(s) 104, 398
BssECI CCNNGG 4 cut(s) 160, 650, 693, 840
BssNI GRCGYC 6 cut(s) 5, 156, 276, 387, 402, 441
Bst2UI CCWGG 2 cut(s) 110, 473
Bst4CI ACNGT 3 cut(s) 531, 586, 856
BstACI GRCGYC 6 cut(s) 5, 156, 276, 387, 402, 441
BstC8I GCNNGC 5 cut(s) 244, 296, 619, 725, 973
BstDSI CCRYGG 2 cut(s) 160, 693
BstF5I GGATG 6 cut(s) 535, 801, 900, 973, 1048, 1105
BstFNI CGCG 2 cut(s) 569, 597
BstH2I RGCGCY 2 cut(s) 89, 405
BstHHI GCGC 4 cut(s) 88, 404, 569, 574
BstMAI GTCTC 1 cut(s) 515
BstMWI GCNNNNNNNGC 7 cut(s) 114, 239, 401, 408, 724, 814, 977
BstNI CCWGG 2 cut(s) 110, 473
BstPAI GACNNNNGTC 1 cut(s) 458
BstSCI CCNGG 4 cut(s) 108, 471, 476, 674
BstSFI CTRYAG 2 cut(s) 716, 803
BstUI CGCG 2 cut(s) 569, 597
BstV1I GCAGC 2 cut(s) 702, 1033
BstX2I RGATCY 5 cut(s) 38, 44, 50, 56, 366
BstYI RGATCY 5 cut(s) 38, 44, 50, 56, 366
BsuI GTATCC 1 cut(s) 1085
BtgI CCRYGG 2 cut(s) 160, 693
BtgZI GCGATG 1 cut(s) 292
BtrI CACGTC 2 cut(s) 349, 436
BtsCI GGATG 6 cut(s) 535, 801, 900, 973, 1048, 1105
BtsIMutI CAGTG 1 cut(s) 574
Cac8I GCNNGC 5 cut(s) 244, 296, 619, 725, 973
CaiI CAGNNNCTG 1 cut(s) 1024
CfoI GCGC 4 cut(s) 88, 404, 569, 574
Cfr10I RCCGGY 2 cut(s) 104, 398
Cfr13I GGNCC 6 cut(s) 241, 453, 480, 498, 690, 697
CseI GACGC 4 cut(s) 62, 183, 284, 449
Csp6I GTAC 3 cut(s) 466, 580, 931
CviAII CATG 3 cut(s) 247, 911, 1096
CviQI GTAC 3 cut(s) 466, 580, 931
DinI GGCGCC 1 cut(s) 403
DraIII CACNNNGTG 1 cut(s) 856
EaeI YGGCCR 2 cut(s) 106, 834
EciI GGCGGA 2 cut(s) 278, 398
Eco147I AGGCCT 1 cut(s) 602
Eco24I GRGCYC 1 cut(s) 612
Eco47I GGWCC 2 cut(s) 453, 480
Eco88I CYCGRG 2 cut(s) 687, 992
EcoO109I RGGNCCY 1 cut(s) 480
EcoRII CCWGG 2 cut(s) 108, 471
EcoT38I GRGCYC 1 cut(s) 612
EgeI GGCGCC 1 cut(s) 403
EheI GGCGCC 1 cut(s) 403
Esp3I CGTCTC 1 cut(s) 515
FaeI CATG 3 cut(s) 250, 914, 1099
FaiI YATR 6 cut(s) 248, 780, 828, 888, 912, 1097
FalI AAGNNNNNCTT 4 cut(s) 716, 748, 917, 949
FaqI GGGAC 1 cut(s) 328
FatI CATG 3 cut(s) 246, 910, 1095
FauI CCCGC 2 cut(s) 251, 513
FbaI TGATCA 1 cut(s) 90
FblI GTMKAC 2 cut(s) 324, 438
Fnu4HI GCNGC 6 cut(s) 292, 336, 555, 570, 716, 1022
FokI GGATG 6 cut(s) 522, 808, 907, 980, 1055, 1112
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 6 cut(s) 292, 336, 555, 570, 716, 1022
GlaI GCGC 4 cut(s) 87, 403, 568, 573
GluI GCNGC 6 cut(s) 292, 336, 555, 570, 716, 1022
GsaI CCCAGC 2 cut(s) 199, 1108
HaeII RGCGCY 2 cut(s) 89, 405
HgaI GACGC 4 cut(s) 62, 183, 284, 449
HhaI GCGC 4 cut(s) 88, 404, 569, 574
Hin1I GRCGYC 6 cut(s) 5, 156, 276, 387, 402, 441
Hin1II CATG 3 cut(s) 250, 914, 1099
Hin6I GCGC 4 cut(s) 86, 402, 567, 572
HinP1I GCGC 4 cut(s) 86, 402, 567, 572
HincII GTYRAC 2 cut(s) 439, 952
HindII GTYRAC 2 cut(s) 439, 952
HinfI GANTC 4 cut(s) 99, 644, 845, 988
HphI GGTGA 5 cut(s) 85, 94, 262, 785, 1039
Hpy166II GTNNAC 6 cut(s) 160, 325, 439, 582, 589, 952
Hpy188I TCNGA 5 cut(s) 173, 487, 520, 946, 960
Hpy188III TCNNGA 5 cut(s) 42, 48, 54, 364, 670
Hpy8I GTNNAC 6 cut(s) 160, 325, 439, 582, 589, 952
HpyAV CCTTC 3 cut(s) 370, 444, 898
HpyCH4III ACNGT 3 cut(s) 531, 586, 856
HpyCH4IV ACGT 4 cut(s) 156, 348, 387, 435
HpyCH4V TGCA 3 cut(s) 621, 718, 971
HpyF10VI GCNNNNNNNGC 7 cut(s) 114, 239, 401, 408, 724, 814, 977
HpySE526I ACGT 4 cut(s) 156, 348, 387, 435
Hsp92I GRCGYC 6 cut(s) 5, 156, 276, 387, 402, 441
Hsp92II CATG 3 cut(s) 250, 914, 1099
HspAI GCGC 4 cut(s) 86, 402, 567, 572
KasI GGCGCC 1 cut(s) 401
Kpn2I TCCGGA 3 cut(s) 41, 47, 53
Ksp22I TGATCA 1 cut(s) 90
LmnI GCTCC 4 cut(s) 70, 122, 182, 258
Lsp1109I GCAGC 2 cut(s) 702, 1033
LweI GCATC 4 cut(s) 789, 958, 1075, 1090
MaeII ACGT 4 cut(s) 156, 348, 387, 435
MaeIII GTNAC 2 cut(s) 670, 773
MboII GAAGA 5 cut(s) 691, 994, 997, 1042, 1058
MflI RGATCY 5 cut(s) 38, 44, 50, 56, 366
MhlI GDGCHC 1 cut(s) 612
MlsI TGGCCA 1 cut(s) 836
MluCI AATT 3 cut(s) 664, 739, 1055
MluNI TGGCCA 1 cut(s) 836
Mly113I GGCGCC 1 cut(s) 402
MlyI GAGTC 1 cut(s) 854
MmeI TCCRAC 5 cut(s) 84, 196, 207, 498, 924
Mox20I TGGCCA 1 cut(s) 836
MroI TCCGGA 3 cut(s) 41, 47, 53
MroXI GAANNNNTTC 1 cut(s) 1074
MscI TGGCCA 1 cut(s) 836
MseI TTAA 1 cut(s) 636
Msp20I TGGCCA 1 cut(s) 836
MspA1I CMGCKG 3 cut(s) 199, 294, 1024
MspR9I CCNGG 4 cut(s) 110, 473, 478, 676
MteI GCGCNGCGC 1 cut(s) 570
Mva1269I GAATGC 2 cut(s) 627, 751
MvaI CCWGG 2 cut(s) 110, 473
MvnI CGCG 2 cut(s) 569, 597
MwoI GCNNNNNNNGC 7 cut(s) 114, 239, 401, 408, 724, 814, 977
NarI GGCGCC 1 cut(s) 402
NciI CCSGG 2 cut(s) 478, 676
NlaIII CATG 3 cut(s) 250, 914, 1099
NlaIV GGNNCC 8 cut(s) 40, 46, 52, 58, 118, 403, 481, 499
NmeAIII GCCGAG 1 cut(s) 675
NmuCI GTSAC 2 cut(s) 670, 773
PceI AGGCCT 1 cut(s) 602
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 2 cut(s) 627, 751
PdmI GAANNNNTTC 1 cut(s) 1074
PfeI GAWTC 3 cut(s) 99, 644, 988
PflFI GACNNNGTC 2 cut(s) 320, 389
PkrI GCNGC 6 cut(s) 293, 337, 556, 571, 717, 1023
PleI GAGTC 1 cut(s) 853
PluTI GGCGCC 1 cut(s) 405
PpsI GAGTC 1 cut(s) 853
PpuMI RGGWCCY 1 cut(s) 480
PshAI GACNNNNGTC 1 cut(s) 458
Psp5II RGGWCCY 1 cut(s) 480
Psp6I CCWGG 2 cut(s) 108, 471
PspFI CCCAGC 2 cut(s) 195, 1104
PspGI CCWGG 2 cut(s) 108, 471
PspN4I GGNNCC 8 cut(s) 40, 46, 52, 58, 118, 403, 481, 499
PspPI GGNCC 6 cut(s) 241, 453, 480, 498, 690, 697
PspPPI RGGWCCY 1 cut(s) 480
PstI CTGCAG 1 cut(s) 720
PstNI CAGNNNCTG 1 cut(s) 1024
PsuI RGATCY 5 cut(s) 38, 44, 50, 56, 366
PsyI GACNNNGTC 2 cut(s) 320, 389
PvuII CAGCTG 1 cut(s) 1024
RsaI GTAC 3 cut(s) 467, 581, 932
RsaNI GTAC 3 cut(s) 466, 580, 931
SalI GTCGAC 1 cut(s) 437
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 6 cut(s) 292, 336, 555, 570, 716, 1022
Sau96I GGNCC 6 cut(s) 241, 453, 480, 498, 690, 697
ScaI AGTACT 1 cut(s) 467
SchI GAGTC 1 cut(s) 854
ScrFI CCNGG 4 cut(s) 110, 473, 478, 676
SduI GDGCHC 1 cut(s) 612
SfaNI GCATC 4 cut(s) 789, 958, 1075, 1090
SfcI CTRYAG 2 cut(s) 716, 803
SfoI GGCGCC 1 cut(s) 403
SgrAI CRCCGGYG 1 cut(s) 398
SgrDI CGTCGACG 1 cut(s) 437
SinI GGWCC 2 cut(s) 453, 480
SmlI CTYRAG 1 cut(s) 934
SmoI CTYRAG 1 cut(s) 934
Sse9I AATT 3 cut(s) 664, 739, 1055
SseBI AGGCCT 1 cut(s) 602
SspDI GGCGCC 1 cut(s) 401
StuI AGGCCT 1 cut(s) 602
StyD4I CCNGG 4 cut(s) 108, 471, 476, 674
TaaI ACNGT 3 cut(s) 531, 586, 856
TaiI ACGT 4 cut(s) 159, 351, 390, 438
TaqI TCGA 7 cut(s) 219, 300, 363, 417, 438, 560, 1053
TasI AATT 3 cut(s) 664, 739, 1055
TatI WGTACW 1 cut(s) 465
TauI GCSGC 4 cut(s) 294, 338, 557, 572
TfiI GAWTC 3 cut(s) 99, 644, 988
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TscAI CASTG 1 cut(s) 581
TseFI GTSAC 2 cut(s) 670, 773
TseI GCWGC 2 cut(s) 715, 1021
Tsp45I GTSAC 2 cut(s) 670, 773
TspDTI ATGAA 4 cut(s) 263, 524, 911, 1059
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 1 cut(s) 581
Tth111I GACNNNGTC 2 cut(s) 320, 389
VpaK11BI GGWCC 2 cut(s) 453, 480
XapI RAATTY 2 cut(s) 664, 739
XmiI GTMKAC 2 cut(s) 324, 438
XmnI GAANNNNTTC 1 cut(s) 1074
ZraI GACGTC 2 cut(s) 157, 388
ZrmI AGTACT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.