Rw2G036350

Growth-regulating factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013899
Physical Location & Seq
Reverse (-)
59739877 .. 59741568
1692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G036350.1

Sequence Viewer

Length: 987 bp
ATGATGAGTGGGGGAAATCCAAGTAGCGCAAGGAATTACAGGTACCCTTTCACAGCAACACAGTGGCAAGAGCTTGAACACCAAGCTCTCATCTTCAAGTACATGGTCTCTGGAGTCCCCATCCCAACTGATCTTGTCTATTCCTTTAAAAGAAGACTGGACTCCTCAATCTCTTCCAGACTCTTCCCTCACCACCCTTTTGGGTGGGGTTGCTATGATATGGGCTTTGGCAGAAAGATAGACCCAGAGCCAGGAAGGTGCAGAAGAACAGATGGTAAAAAATGGAGGTGTTCAAAGGAAGCATACCCAGATTCTAAGTACTGTGAGAGGCATATGCACAGAGGCAGAAACCGTTCAAGAAAGCCTGTGGAAGCTTCTTCTAGTTCTTCTACTACAACTCCTAATAATAATAATAATACTATTCCAACACCTCCAGAGTACAACCCTGCTTCAATCCACAAAAACCTCTCCAAAACCTCCCCTTCAATGTTTTCCTCATCTTTCACCAATGAAACACCTCACACCCAACACCAAAGTTCATCTCTTTATCCATTCCTTTCTCGGCCACAGGGTTCCGGTACCAACCCTCAACTTTTTCTCAACTCTGGATCTTATGTTAGTGAAGCTGATCACAGAGATTACAGGTATCTTCAAGGAACAAAGGAAGGTGTGGATCATGAGCTAGCCTTCTTTCCTGCAGAGTCTTCTGCAAATGCAAGAAGTTTAATGAGCTCCTATAAAGGCTACTCATCCCAATCTCAGTTTCAAAGCCTCACAGATCATTATCCACAGCAGCAATCCCAGGATGAGCAGCATTGCTATGTTTTGGGCACTGATTTCAAATCAAGCAGACCAATCAAAACAGAGAATAAAGAAGCTGATCAGACCCAAAAGTCCCTCCACCATTTCTTTGGAGACTGGCCTCCAAAGTCCACAGACACCTGGCTTGATCTTGCACCCAATTCCAGACTTCATAATGGTAATTAG

Protein Analysis

328

Amino Acids

37.39

Weight (kDa)

9.05

Isoelectric Point (pI)

58.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QLQ PF08880 16 - 46 2.3e-14 QLQ
WRC PF08879 82 - 124 3.2e-21 WRC
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013899)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13960
fragaria_vesca FvH4_6g34730 FvH4_6g34730 FvH4_6g34730
malus_domestica MD09G1183300.v1.1
prunus_persica Prupe.3G041800_v2.0.a1
pyrus_communis pycom09g10100
rosa_chinensis RchiOBHm_Chr2g0145381
rosa_laevigata RLG00000020139
rosa_multiflora Rmu_sc0001298.1_g000050
rosa_roxburghii Rroxscaffold_2G00100740
rosa_rugosa Rorug02G0391800
rosa_samantha Rh2AG445100 Rh2BG455900 Rh2CG431900 Rh2DG465800
rosa_wichuraiana Rw2G036350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 892
Acc65I GGTACC 2 cut(s) 42, 578
AccB1I GGYRCC 2 cut(s) 42, 578
AclWI GGATC 2 cut(s) 616, 681
AcoI YGGCCR 1 cut(s) 563
AfaI GTAC 5 cut(s) 44, 101, 320, 440, 580
AfiI CCNNNNNNNGG 1 cut(s) 251
AgsI TTSAA 9 cut(s) 77, 97, 294, 357, 453, 486, 653, 767, 841
AjnI CCWGG 3 cut(s) 250, 801, 941
AjuI GAANNNNNNNTTGG 2 cut(s) 747, 779
AluBI AGCT 7 cut(s) 73, 86, 374, 626, 682, 732, 878
AluI AGCT 7 cut(s) 73, 86, 374, 626, 682, 732, 878
Alw21I GWGCWC 1 cut(s) 734
Alw26I GTCTC 2 cut(s) 112, 909
AlwI GGATC 2 cut(s) 616, 681
AoxI GGCC 2 cut(s) 563, 920
ApeKI GCWGC 2 cut(s) 793, 811
Asp700I GAANNNNTTC 1 cut(s) 352
Asp718I GGTACC 2 cut(s) 42, 578
AspLEI GCGC 1 cut(s) 29
AsuHPI GGTGA 2 cut(s) 182, 496
AsuNHI GCTAGC 1 cut(s) 682
BaeGI GKGCMC 1 cut(s) 833
BanI GGYRCC 2 cut(s) 42, 578
BanII GRGCYC 1 cut(s) 734
BbsI GAAGAC 2 cut(s) 160, 696
Bbv12I GWGCWC 1 cut(s) 734
BbvI GCAGC 2 cut(s) 805, 823
BccI CCATC 2 cut(s) 128, 266
BciT130I CCWGG 3 cut(s) 252, 803, 943
BclI TGATCA 2 cut(s) 628, 880
BcoDI GTCTC 2 cut(s) 112, 909
BfaI CTAG 2 cut(s) 381, 683
BfmI CTRYAG 1 cut(s) 696
BisI GCNGC 2 cut(s) 794, 812
BlsI GCNGC 2 cut(s) 795, 813
BmcAI AGTACT 1 cut(s) 320
Bme1390I CCNGG 3 cut(s) 252, 803, 943
BmiI GGNNCC 3 cut(s) 44, 574, 580
BmrFI CCNGG 3 cut(s) 252, 803, 943
BmtI GCTAGC 1 cut(s) 686
BpiI GAAGAC 2 cut(s) 160, 696
BpmI CTGGAG 2 cut(s) 132, 417
BsaBI GATNNNNATC 1 cut(s) 783
BsaI GGTCTC 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 801
BsaWI WCCGGW 1 cut(s) 575
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bsc4I CCNNNNNNNGG 1 cut(s) 251
Bse1I ACTGG 2 cut(s) 162, 923
Bse3DI GCAATG 1 cut(s) 814
Bse8I GATNNNNATC 1 cut(s) 783
BseBI CCWGG 3 cut(s) 252, 803, 943
BseDI CCNNGG 1 cut(s) 801
BseGI GGATG 3 cut(s) 120, 749, 811
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 1 cut(s) 251
BseMI GCAATG 1 cut(s) 814
BseMII CTCAG 1 cut(s) 773
BseNI ACTGG 2 cut(s) 162, 923
BseRI GAGGAG 1 cut(s) 154
BseSI GKGCMC 1 cut(s) 833
BseXI GCAGC 2 cut(s) 805, 823
BsgI GTGCAG 1 cut(s) 280
BshFI GGCC 2 cut(s) 565, 922
BshNI GGYRCC 2 cut(s) 42, 578
BsiHKAI GWGCWC 1 cut(s) 734
BsiSI CCGG 1 cut(s) 576
BslFI GGGAC 2 cut(s) 101, 880
BslI CCNNNNNNNGG 1 cut(s) 251
BsmAI GTCTC 2 cut(s) 112, 909
BsmFI GGGAC 2 cut(s) 101, 880
BsnI GGCC 2 cut(s) 565, 922
Bso31I GGTCTC 1 cut(s) 112
Bsp1286I GDGCHC 2 cut(s) 734, 833
Bsp143I GATC 7 cut(s) 130, 608, 628, 673, 778, 880, 949
BspANI GGCC 2 cut(s) 565, 922
BspCNI CTCAG 1 cut(s) 772
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 3 cut(s) 44, 574, 580
BspMAI CTGCAG 1 cut(s) 700
BspOI GCTAGC 1 cut(s) 686
BspPI GGATC 2 cut(s) 616, 681
BspT107I GGYRCC 2 cut(s) 42, 578
BspTNI GGTCTC 1 cut(s) 112
BsrDI GCAATG 1 cut(s) 814
BsrI ACTGG 2 cut(s) 162, 923
BssECI CCNNGG 1 cut(s) 801
BssMI GATC 7 cut(s) 130, 608, 628, 673, 778, 880, 949
Bst2UI CCWGG 3 cut(s) 252, 803, 943
Bst4CI ACNGT 3 cut(s) 63, 323, 353
Bst6I CTCTTC 2 cut(s) 178, 188
BstC8I GCNNGC 1 cut(s) 684
BstDEI CTNAG 2 cut(s) 315, 759
BstF5I GGATG 3 cut(s) 120, 749, 811
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 7 cut(s) 133, 611, 631, 676, 781, 883, 952
BstMAI GTCTC 2 cut(s) 112, 909
BstMBI GATC 7 cut(s) 130, 608, 628, 673, 778, 880, 949
BstNI CCWGG 3 cut(s) 252, 803, 943
BstSCI CCNGG 3 cut(s) 250, 801, 941
BstSFI CTRYAG 1 cut(s) 696
BstSLI GKGCMC 1 cut(s) 833
BstV1I GCAGC 2 cut(s) 805, 823
BstV2I GAAGAC 2 cut(s) 160, 696
BstX2I RGATCY 1 cut(s) 608
BstXI CCANNNNNNTGG 2 cut(s) 200, 911
BstYI RGATCY 1 cut(s) 608
BsuRI GGCC 2 cut(s) 565, 922
BtsCI GGATG 3 cut(s) 120, 749, 811
BtsIMutI CAGTG 2 cut(s) 68, 831
Cac8I GCNNGC 1 cut(s) 684
CciI TCATGA 1 cut(s) 676
CfoI GCGC 1 cut(s) 29
Csp6I GTAC 5 cut(s) 43, 100, 319, 439, 579
CviAII CATG 2 cut(s) 103, 677
CviQI GTAC 5 cut(s) 43, 100, 319, 439, 579
DdeI CTNAG 2 cut(s) 315, 759
DpnI GATC 7 cut(s) 132, 610, 630, 675, 780, 882, 951
DpnII GATC 7 cut(s) 130, 608, 628, 673, 778, 880, 949
DraI TTTAAA 1 cut(s) 148
DrdI GACNNNNNNGTC 1 cut(s) 892
DseDI GACNNNNNNGTC 1 cut(s) 892
EaeI YGGCCR 1 cut(s) 563
Eam1104I CTCTTC 2 cut(s) 178, 188
EarI CTCTTC 2 cut(s) 178, 188
Ecl136II GAGCTC 1 cut(s) 732
Eco24I GRGCYC 1 cut(s) 734
Eco31I GGTCTC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 732
EcoICRI GAGCTC 1 cut(s) 732
EcoRII CCWGG 3 cut(s) 250, 801, 941
EcoT38I GRGCYC 1 cut(s) 734
FaeI CATG 2 cut(s) 106, 680
FaqI GGGAC 2 cut(s) 101, 880
FatI CATG 2 cut(s) 102, 676
FauNDI CATATG 1 cut(s) 333
FbaI TGATCA 2 cut(s) 628, 880
Fnu4HI GCNGC 2 cut(s) 794, 812
FokI GGATG 3 cut(s) 107, 736, 818
FriOI GRGCYC 1 cut(s) 734
Fsp4HI GCNGC 2 cut(s) 794, 812
FspBI CTAG 2 cut(s) 381, 683
GlaI GCGC 1 cut(s) 28
GluI GCNGC 2 cut(s) 794, 812
GsuI CTGGAG 2 cut(s) 132, 417
HaeIII GGCC 2 cut(s) 565, 922
HapII CCGG 1 cut(s) 576
HhaI GCGC 1 cut(s) 29
Hin1II CATG 2 cut(s) 106, 680
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HindIII AAGCTT 1 cut(s) 372
HinfI GANTC 5 cut(s) 114, 161, 180, 311, 701
HpaII CCGG 1 cut(s) 576
HphI GGTGA 2 cut(s) 182, 496
Hpy166II GTNNAC 1 cut(s) 933
Hpy188I TCNGA 1 cut(s) 885
Hpy188III TCNNGA 7 cut(s) 111, 177, 357, 434, 606, 677, 966
Hpy8I GTNNAC 1 cut(s) 933
HpyAV CCTTC 4 cut(s) 249, 492, 659, 697
HpyCH4III ACNGT 3 cut(s) 63, 323, 353
HpyCH4V TGCA 6 cut(s) 261, 337, 698, 710, 716, 956
HpyF3I CTNAG 2 cut(s) 315, 759
Hsp92II CATG 2 cut(s) 106, 680
HspAI GCGC 1 cut(s) 27
KpnI GGTACC 2 cut(s) 46, 582
Ksp22I TGATCA 2 cut(s) 628, 880
Kzo9I GATC 7 cut(s) 130, 608, 628, 673, 778, 880, 949
LmnI GCTCC 1 cut(s) 737
Lsp1109I GCAGC 2 cut(s) 805, 823
MaeI CTAG 2 cut(s) 381, 683
MalI GATC 7 cut(s) 132, 610, 630, 675, 780, 882, 951
MboI GATC 7 cut(s) 130, 608, 628, 673, 778, 880, 949
MboII GAAGA 9 cut(s) 85, 165, 165, 175, 276, 369, 378, 641, 696
MflI RGATCY 1 cut(s) 608
MhlI GDGCHC 2 cut(s) 734, 833
MluCI AATT 3 cut(s) 34, 961, 982
MlyI GAGTC 4 cut(s) 123, 155, 174, 710
MmeI TCCRAC 1 cut(s) 449
MroXI GAANNNNTTC 1 cut(s) 352
MseI TTAA 2 cut(s) 147, 725
MslI CAYNNNNRTG 1 cut(s) 819
MspI CCGG 1 cut(s) 576
MspR9I CCNGG 3 cut(s) 252, 803, 943
MvaI CCWGG 3 cut(s) 252, 803, 943
NdeI CATATG 1 cut(s) 333
NdeII GATC 7 cut(s) 130, 608, 628, 673, 778, 880, 949
NheI GCTAGC 1 cut(s) 682
NlaIII CATG 2 cut(s) 106, 680
NlaIV GGNNCC 3 cut(s) 44, 574, 580
NmeAIII GCCGAG 1 cut(s) 541
PagI TCATGA 1 cut(s) 676
PdmI GAANNNNTTC 1 cut(s) 352
PfeI GAWTC 1 cut(s) 311
PkrI GCNGC 2 cut(s) 795, 813
PleI GAGTC 4 cut(s) 122, 155, 174, 709
PpsI GAGTC 4 cut(s) 122, 155, 174, 709
Psp124BI GAGCTC 1 cut(s) 734
Psp6I CCWGG 3 cut(s) 250, 801, 941
PspGI CCWGG 3 cut(s) 250, 801, 941
PspN4I GGNNCC 3 cut(s) 44, 574, 580
PstI CTGCAG 1 cut(s) 700
PsuI RGATCY 1 cut(s) 608
RsaI GTAC 5 cut(s) 44, 101, 320, 440, 580
RsaNI GTAC 5 cut(s) 43, 100, 319, 439, 579
RseI CAYNNNNRTG 1 cut(s) 819
SacI GAGCTC 1 cut(s) 734
SaqAI TTAA 2 cut(s) 147, 725
SatI GCNGC 2 cut(s) 794, 812
Sau3AI GATC 7 cut(s) 130, 608, 628, 673, 778, 880, 949
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 4 cut(s) 123, 155, 174, 710
ScrFI CCNGG 3 cut(s) 252, 803, 943
SduI GDGCHC 2 cut(s) 734, 833
SfcI CTRYAG 1 cut(s) 696
SmiMI CAYNNNNRTG 1 cut(s) 819
Sse9I AATT 3 cut(s) 34, 961, 982
SspMI CTAG 2 cut(s) 381, 683
SstI GAGCTC 1 cut(s) 734
StyD4I CCNGG 3 cut(s) 250, 801, 941
TaaI ACNGT 3 cut(s) 63, 323, 353
TasI AATT 3 cut(s) 34, 961, 982
TatI WGTACW 3 cut(s) 99, 318, 438
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 2 cut(s) 147, 725
Tru9I TTAA 2 cut(s) 147, 725
TscAI CASTG 2 cut(s) 68, 838
TseI GCWGC 2 cut(s) 793, 811
TspDTI ATGAA 3 cut(s) 525, 528, 962
TspRI CASTG 2 cut(s) 68, 838
XcmI CCANNNNNNNNNTGG 1 cut(s) 908
XmnI GAANNNNTTC 1 cut(s) 352
XspI CTAG 2 cut(s) 381, 683
ZrmI AGTACT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.