Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0012097

Physical Location & Seq

Genomic Coordinates Forward (+)

80686607 .. 80688863

Gene Structure 2257 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G052740.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1410 bp

ATGGAAGATGAACACGCCCCTGAACAAGTTCCACTAAAACAAGCTATCCCTGCTCATCTCTTTGCTTTACCAGTTGATGAAGATGGAAAAGCCACAGAATTCAGACCATTCTCAATGGCATCACCACACATGCGAGCCTTCCACCTCGCATGGTTCTCTTTATTCTCTTGCTTCTTCTCCACCTTCTCCATCCCTCCACTCCTCGCCGTCATCCGCGACGACCTCCACCTCACCGAAACCGACACCTCACGCGCCGCCATCGCAGCCTTCATCGGCTCCATCTTCTCCCGCATTGCCATGGGCCCCATCTGCGACCACCTAGGCCCACGCGTCGCCTCTGCCACCCTCTCCCTCCTCACCTCCCCCATCATCCTCGGCACCGCCCTCGTCTCCTCCCCAAACGCCTTCATCACCATCCGCTTCCTCGTCGGCTTCTCCCTCGCCAACTTCGTCGCCAACCAGTTCTGGATGAGCCGCATGTTCTCAGGCTGCGTCGTCGGCCTCGCCAATGGGGTCTCCGCCGGCTGGGCCAACACCGGCGCCGGCGCCACCCAGCTTGTCATGCCACTCATCTACTCCTTTATCATGTCAGCATTCAACTTGCCATCCTCCGCTGCCTGGCGACTGTCATATATAGTCCCCGCCGTGCTCCAAGCCAGCACAGCTGTACTGGTCCTGACCTACGGCCAGGACTTACCTTCTGGAAGCTACAAACGTACCTCCAAGAATGGCTCGAAAGACAGCTTTTTCGAGGTCTTCTTCCACGGGATAAAAAACTACAGAGGTTGGATCTTGGCTTTGACCTACGGGTTTTCCTTCGGTGTGGAGCTGACCACTGACAACATCATTGCTCAGTACTTCTACGACAGGTTCGGCGTGAACATTCAGGTGGCCGGAACCATAGCTTCCTGCTTCGGATTGGCGAACTTCTTTTCCAGGCCGAGCGGGGGTTTGTTTTCCGATATGTTGGGGAGGAGGTTCGGGATGAGAGGGAGGCTGTGGGGTTTGTGGGCGACGCAAACTGTGGCGGGTTTGCTGTGCTTGTTACTCGGCCGAGTTAACTCTCTCTGGAGCTCCATACTTGTCATGTGTGTGTTCTCAGTTTTTGTTCAGGCCGCCGCGGGACTCACGTTTGGGGTGGTTCCATTTGTTTCCAAAAGGTCACTAGGAGTGATTTCAGGGATAACAGGCGGTGGAGGGACTATGGGGGCGGTGATAACCCAGCTCCTATTGTTTTCAGGCTCTAAATACTCGAAGCAAACAGGCATTTCTCTTATGGGAATCATGATGATCGTTTGTACTCTCCCACTGTCACTCATGTACTTCCCTCAATGGGGTGGAATGCTCTGTGGCCCTGCCCATCATCCTGATCTCGATCCAGAAGCAGATCTTTATCGCTTGCTCAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0001101 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008509 GO:0009705 GO:0009987 GO:0010035 GO:0010167 GO:0015075 GO:0015103 GO:0015112 GO:0015318 GO:0015698 GO:0015706 GO:0016020 GO:0022857 GO:0031090 GO:0034220 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0070887 GO:0071229 GO:0071241 GO:0071249 GO:0071705 GO:0071944 GO:0098588 GO:0098656 GO:0098805 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170

KEGG Pathways

Metabolic & Signaling

ko00910 map00910

Pfam Domains

Protein Families

MFS_1

Protein Analysis

469

Amino Acids

50.73

Weight (kDa)

8.89

Isoelectric Point (pI)

36.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFS_1	PF07690	51 - 406	3.1e-21	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012097)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14570
fragaria_vesca	FvH4_6g51160
malus_domestica	MD09G1028900.v1.1 MD17G1030600.v1.1
prunus_persica	Prupe.3G289200_v2.0.a1
pyrus_communis	pycom111g02290 pycom17g02800 pycom17g02810
rosa_chinensis	RchiOBHm_Chr2g0172751
rosa_laevigata	RLG00000022130
rosa_multiflora	Rmu_co8072536.1_g000001 Rmu_sc0010744.1_g000005
rosa_roxburghii	Rroxscaffold_2G00079560
rosa_rugosa	Rorug02G0561200
rosa_samantha	Rh2AG638400 Rh2BG652000 Rh2CG618300 Rh2DG666000
rosa_wichuraiana	Rw2G052740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	3 cut(s)	377, 539, 545
AccBSI	CCGCTC	1 cut(s)	945
AccII	CGCG	4 cut(s)	216, 252, 330, 1121
AclWI	GGATC	2 cut(s)	797, 1370
AcoI	YGGCCR	3 cut(s)	685, 891, 1051
AcsI	RAATTY	1 cut(s)	98
AcyI	GRCGYC	2 cut(s)	540, 546
AfaI	GTAC	5 cut(s)	669, 718, 857, 1300, 1322
AfiI	CCNNNNNNNGG	5 cut(s)	525, 618, 947, 1333, 1334
AflIII	ACRYGT	1 cut(s)	328
AgsI	TTSAA	1 cut(s)	598
AjnI	CCWGG	3 cut(s)	617, 687, 935
AloI	GAACNNNNNNTCC	2 cut(s)	917, 949
AluBI	AGCT	9 cut(s)	44, 556, 665, 708, 744, 829, 905, 1074, 1225
AluI	AGCT	9 cut(s)	44, 556, 665, 708, 744, 829, 905, 1074, 1225
Alw21I	GWGCWC	2 cut(s)	651, 1076
Alw26I	GTCTC	2 cut(s)	394, 520
AlwI	GGATC	2 cut(s)	797, 1370
ApaI	GGGCCC	1 cut(s)	305
ApeKI	GCWGC	3 cut(s)	263, 489, 614
ApoI	RAATTY	1 cut(s)	98
ArsI	GACNNNNNNTTYG	2 cut(s)	731, 763
Asp700I	GAANNNNTTC	1 cut(s)	27
AspA2I	CCTAGG	1 cut(s)	319
AspLEI	GCGC	3 cut(s)	254, 542, 548
AspS9I	GGNCC	6 cut(s)	301, 302, 323, 528, 673, 1352
AsuHPI	GGTGA	5 cut(s)	114, 223, 349, 403, 1225
AvaII	GGWCC	1 cut(s)	673
AvrII	CCTAGG	1 cut(s)	319
BaeGI	GKGCMC	1 cut(s)	305
BaeI	ACNNNNGTAYC	2 cut(s)	700, 733
BanI	GGYRCC	3 cut(s)	377, 539, 545
BanII	GRGCYC	2 cut(s)	305, 1076
BbsI	GAAGAC	1 cut(s)	748
Bbv12I	GWGCWC	2 cut(s)	651, 1076
BbvI	GCAGC	3 cut(s)	275, 476, 601
BccI	CCATC	9 cut(s)	77, 197, 266, 287, 314, 374, 422, 613, 1368
BceAI	ACGGC	3 cut(s)	191, 629, 700
BcgI	CGANNNNNNTGC	2 cut(s)	367, 401
BciT130I	CCWGG	3 cut(s)	619, 689, 937
BcoDI	GTCTC	2 cut(s)	394, 520
BfaI	CTAG	2 cut(s)	320, 1166
BfmI	CTRYAG	1 cut(s)	778
BfoI	RGCGCY	2 cut(s)	543, 549
BglI	GCCNNNNNGGC	1 cut(s)	527
BglII	AGATCT	1 cut(s)	1387
BisI	GCNGC	7 cut(s)	255, 264, 475, 490, 615, 1116, 1119
BlnI	CCTAGG	1 cut(s)	319
BlsI	GCNGC	7 cut(s)	256, 265, 476, 491, 616, 1117, 1120
BmcAI	AGTACT	1 cut(s)	857
Bme1390I	CCNGG	3 cut(s)	619, 689, 937
Bme18I	GGWCC	1 cut(s)	673
BmgT120I	GGNCC	6 cut(s)	301, 302, 323, 528, 673, 1352
BmiI	GGNNCC	8 cut(s)	277, 303, 304, 379, 541, 547, 898, 1143
BmrFI	CCNGG	3 cut(s)	619, 689, 937
BmsI	GCATC	1 cut(s)	128
BpiI	GAAGAC	1 cut(s)	748
BpmI	CTGGAG	1 cut(s)	1090
BsaBI	GATNNNNATC	2 cut(s)	1374, 1392
BsaHI	GRCGYC	2 cut(s)	540, 546
BsaI	GGTCTC	1 cut(s)	520
BsaJI	CCNNGG	5 cut(s)	297, 319, 373, 763, 1119
Bsc4I	CCNNNNNNNGG	5 cut(s)	525, 618, 947, 1333, 1334
Bse118I	RCCGGY	3 cut(s)	521, 536, 542
Bse1I	ACTGG	3 cut(s)	71, 460, 675
Bse3DI	GCAATG	2 cut(s)	291, 846
Bse8I	GATNNNNATC	2 cut(s)	1374, 1392
BseBI	CCWGG	3 cut(s)	619, 689, 937
BseDI	CCNNGG	5 cut(s)	297, 319, 373, 763, 1119
BseGI	GGATG	8 cut(s)	189, 210, 369, 414, 474, 605, 990, 1363
BseJI	GATNNNNATC	2 cut(s)	1374, 1392
BseLI	CCNNNNNNNGG	5 cut(s)	525, 618, 947, 1333, 1334
BseMI	GCAATG	2 cut(s)	291, 846
BseMII	CTCAG	3 cut(s)	498, 866, 1113
BseNI	ACTGG	3 cut(s)	71, 460, 675
BseRI	GAGGAG	4 cut(s)	191, 344, 382, 988
BseSI	GKGCMC	1 cut(s)	305
BseX3I	CGGCCG	1 cut(s)	1051
BseXI	GCAGC	3 cut(s)	275, 476, 601
BseYI	CCCAGC	3 cut(s)	525, 552, 1221
Bsh1236I	CGCG	4 cut(s)	216, 252, 330, 1121
Bsh1285I	CGRYCG	1 cut(s)	1054
BshNI	GGYRCC	3 cut(s)	377, 539, 545
BsiEI	CGRYCG	1 cut(s)	1054
BsiHKAI	GWGCWC	2 cut(s)	651, 1076
BsiSI	CCGG	4 cut(s)	522, 537, 543, 894
BslFI	GGGAC	3 cut(s)	623, 1137, 1213
BslI	CCNNNNNNNGG	5 cut(s)	525, 618, 947, 1333, 1334
BsmAI	GTCTC	2 cut(s)	394, 520
BsmBI	CGTCTC	1 cut(s)	394
BsmFI	GGGAC	3 cut(s)	623, 1137, 1213
BsmI	GAATGC	2 cut(s)	593, 1347
Bso31I	GGTCTC	1 cut(s)	520
Bsp120I	GGGCCC	1 cut(s)	301
Bsp1286I	GDGCHC	3 cut(s)	305, 651, 1076
Bsp143I	GATC	5 cut(s)	789, 1290, 1369, 1375, 1387
Bsp19I	CCATGG	1 cut(s)	297
BspCNI	CTCAG	3 cut(s)	497, 865, 1112
BspFNI	CGCG	4 cut(s)	216, 252, 330, 1121
BspHI	TCATGA	1 cut(s)	1284
BspLI	GGNNCC	8 cut(s)	277, 303, 304, 379, 541, 547, 898, 1143
BspPI	GGATC	2 cut(s)	797, 1370
BspT107I	GGYRCC	3 cut(s)	377, 539, 545
BspTNI	GGTCTC	1 cut(s)	520
BsrBI	CCGCTC	1 cut(s)	945
BsrDI	GCAATG	2 cut(s)	291, 846
BsrFI	RCCGGY	3 cut(s)	521, 536, 542
BsrI	ACTGG	3 cut(s)	71, 460, 675
BssAI	RCCGGY	3 cut(s)	521, 536, 542
BssECI	CCNNGG	5 cut(s)	297, 319, 373, 763, 1119
BssMI	GATC	5 cut(s)	789, 1290, 1369, 1375, 1387
BssNI	GRCGYC	2 cut(s)	540, 546
BssT1I	CCWWGG	2 cut(s)	297, 319
Bst2UI	CCWGG	3 cut(s)	619, 689, 937
Bst4CI	ACNGT	3 cut(s)	627, 1024, 1311
BstACI	GRCGYC	2 cut(s)	540, 546
BstC8I	GCNNGC	5 cut(s)	135, 523, 544, 658, 1400
BstDEI	CTNAG	3 cut(s)	484, 852, 1099
BstDSI	CCRYGG	3 cut(s)	297, 763, 1119
BstF5I	GGATG	8 cut(s)	189, 210, 369, 414, 474, 605, 990, 1363
BstFNI	CGCG	4 cut(s)	216, 252, 330, 1121
BstH2I	RGCGCY	2 cut(s)	543, 549
BstHHI	GCGC	3 cut(s)	254, 542, 548
BstKTI	GATC	5 cut(s)	792, 1293, 1372, 1378, 1390
BstMAI	GTCTC	2 cut(s)	394, 520
BstMBI	GATC	5 cut(s)	789, 1290, 1369, 1375, 1387
BstMCI	CGRYCG	1 cut(s)	1054
BstMWI	GCNNNNNNNGC	8 cut(s)	50, 260, 263, 309, 498, 527, 562, 662
BstNI	CCWGG	3 cut(s)	619, 689, 937
BstNSI	RCATGY	2 cut(s)	133, 481
BstSCI	CCNGG	3 cut(s)	617, 687, 935
BstSFI	CTRYAG	1 cut(s)	778
BstSLI	GKGCMC	1 cut(s)	305
BstUI	CGCG	4 cut(s)	216, 252, 330, 1121
BstV1I	GCAGC	3 cut(s)	275, 476, 601
BstV2I	GAAGAC	1 cut(s)	748
BstX2I	RGATCY	2 cut(s)	789, 1387
BstYI	RGATCY	2 cut(s)	789, 1387
BstZI	CGGCCG	1 cut(s)	1051
BtgI	CCRYGG	3 cut(s)	297, 763, 1119
BtgZI	GCGATG	1 cut(s)	244
BtsCI	GGATG	8 cut(s)	189, 210, 369, 414, 474, 605, 990, 1363
BtsIMutI	CAGTG	2 cut(s)	834, 1307
Cac8I	GCNNGC	5 cut(s)	135, 523, 544, 658, 1400
CciI	TCATGA	1 cut(s)	1284
CfoI	GCGC	3 cut(s)	254, 542, 548
Cfr10I	RCCGGY	3 cut(s)	521, 536, 542
Cfr13I	GGNCC	6 cut(s)	301, 302, 323, 528, 673, 1352
Cfr42I	CCGCGG	1 cut(s)	1122
CseI	GACGC	3 cut(s)	319, 481, 1024
Csp6I	GTAC	5 cut(s)	668, 717, 856, 1299, 1321
CspCI	CAANNNNNGTGG	2 cut(s)	538, 573
CviAII	CATG	9 cut(s)	130, 150, 298, 478, 562, 586, 1087, 1285, 1318
CviQI	GTAC	5 cut(s)	668, 717, 856, 1299, 1321
DdeI	CTNAG	3 cut(s)	484, 852, 1099
DinI	GGCGCC	2 cut(s)	541, 547
DpnI	GATC	5 cut(s)	791, 1292, 1371, 1377, 1389
DpnII	GATC	5 cut(s)	789, 1290, 1369, 1375, 1387
EaeI	YGGCCR	3 cut(s)	685, 891, 1051
EagI	CGGCCG	1 cut(s)	1051
EciI	GGCGGA	1 cut(s)	508
Ecl136II	GAGCTC	1 cut(s)	1074
EclXI	CGGCCG	1 cut(s)	1051
Eco130I	CCWWGG	2 cut(s)	297, 319
Eco24I	GRGCYC	2 cut(s)	305, 1076
Eco31I	GGTCTC	1 cut(s)	520
Eco47I	GGWCC	1 cut(s)	673
Eco52I	CGGCCG	1 cut(s)	1051
Eco53kI	GAGCTC	1 cut(s)	1074
EcoICRI	GAGCTC	1 cut(s)	1074
EcoO109I	RGGNCCY	1 cut(s)	302
EcoRI	GAATTC	1 cut(s)	98
EcoRII	CCWGG	3 cut(s)	617, 687, 935
EcoT14I	CCWWGG	2 cut(s)	297, 319
EcoT38I	GRGCYC	2 cut(s)	305, 1076
EgeI	GGCGCC	2 cut(s)	541, 547
EheI	GGCGCC	2 cut(s)	541, 547
ErhI	CCWWGG	2 cut(s)	297, 319
Esp3I	CGTCTC	1 cut(s)	394
FaeI	CATG	9 cut(s)	133, 153, 301, 481, 565, 589, 1090, 1288, 1321
FalI	AAGNNNNNCTT	2 cut(s)	1374, 1406
FaqI	GGGAC	3 cut(s)	623, 1137, 1213
FatI	CATG	9 cut(s)	129, 149, 297, 477, 561, 585, 1086, 1284, 1317
FauI	CCCGC	5 cut(s)	296, 649, 938, 1021, 1114
Fnu4HI	GCNGC	7 cut(s)	255, 264, 475, 490, 615, 1116, 1119
FokI	GGATG	8 cut(s)	176, 197, 356, 401, 481, 592, 997, 1350
FriOI	GRGCYC	2 cut(s)	305, 1076
Fsp4HI	GCNGC	7 cut(s)	255, 264, 475, 490, 615, 1116, 1119
FspBI	CTAG	2 cut(s)	320, 1166
GlaI	GCGC	3 cut(s)	253, 541, 547
GluI	GCNGC	7 cut(s)	255, 264, 475, 490, 615, 1116, 1119
GsaI	CCCAGC	3 cut(s)	529, 556, 1225
GsuI	CTGGAG	1 cut(s)	1090
HaeII	RGCGCY	2 cut(s)	543, 549
HapII	CCGG	4 cut(s)	522, 537, 543, 894
HgaI	GACGC	3 cut(s)	319, 481, 1024
HhaI	GCGC	3 cut(s)	254, 542, 548
Hin1I	GRCGYC	2 cut(s)	540, 546
Hin1II	CATG	9 cut(s)	133, 153, 301, 481, 565, 589, 1090, 1288, 1321
Hin6I	GCGC	3 cut(s)	252, 540, 546
HinP1I	GCGC	3 cut(s)	252, 540, 546
HincII	GTYRAC	1 cut(s)	1060
HindII	GTYRAC	1 cut(s)	1060
HinfI	GANTC	2 cut(s)	1125, 1281
HpaI	GTTAAC	1 cut(s)	1060
HpaII	CCGG	4 cut(s)	522, 537, 543, 894
HphI	GGTGA	5 cut(s)	114, 223, 349, 403, 1225
Hpy166II	GTNNAC	2 cut(s)	880, 1060
Hpy188I	TCNGA	3 cut(s)	104, 917, 961
Hpy188III	TCNNGA	9 cut(s)	466, 676, 702, 982, 1069, 1285, 1367, 1373, 1379
Hpy8I	GTNNAC	2 cut(s)	880, 1060
Hpy99I	CGWCG	7 cut(s)	221, 335, 431, 455, 497, 500, 1018
HpyAV	CCTTC	6 cut(s)	148, 193, 277, 415, 708, 826
HpyCH4III	ACNGT	3 cut(s)	627, 1024, 1311
HpyCH4IV	ACGT	2 cut(s)	715, 1130
HpyF10VI	GCNNNNNNNGC	8 cut(s)	50, 260, 263, 309, 498, 527, 562, 662
HpyF3I	CTNAG	3 cut(s)	484, 852, 1099
HpySE526I	ACGT	2 cut(s)	715, 1130
Hsp92I	GRCGYC	2 cut(s)	540, 546
Hsp92II	CATG	9 cut(s)	133, 153, 301, 481, 565, 589, 1090, 1288, 1321
HspAI	GCGC	3 cut(s)	252, 540, 546
KasI	GGCGCC	2 cut(s)	539, 545
KroI	GCCGGC	2 cut(s)	521, 542
KroNI	GCCGGC	2 cut(s)	523, 544
KspAI	GTTAAC	1 cut(s)	1060
KspI	CCGCGG	1 cut(s)	1122
Kzo9I	GATC	5 cut(s)	789, 1290, 1369, 1375, 1387
LmnI	GCTCC	6 cut(s)	281, 654, 826, 1071, 1079, 1230
Lsp1109I	GCAGC	3 cut(s)	275, 476, 601
LweI	GCATC	1 cut(s)	128
MaeI	CTAG	2 cut(s)	320, 1166
MaeII	ACGT	2 cut(s)	715, 1130
MaeIII	GTNAC	3 cut(s)	1044, 1161, 1311
MalI	GATC	5 cut(s)	791, 1292, 1371, 1377, 1389
MbiI	CCGCTC	1 cut(s)	945
MboI	GATC	5 cut(s)	789, 1290, 1369, 1375, 1387
MboII	GAAGA	6 cut(s)	17, 92, 166, 274, 748, 751
MflI	RGATCY	2 cut(s)	789, 1387
MhlI	GDGCHC	3 cut(s)	305, 651, 1076
MluCI	AATT	1 cut(s)	98
MluI	ACGCGT	1 cut(s)	328
Mly113I	GGCGCC	2 cut(s)	540, 546
MlyI	GAGTC	1 cut(s)	1119
MmeI	TCCRAC	1 cut(s)	767
MreI	CGCCGGCG	1 cut(s)	542
MroNI	GCCGGC	2 cut(s)	521, 542
MroXI	GAANNNNTTC	1 cut(s)	27
MseI	TTAA	1 cut(s)	1059
MslI	CAYNNNNRTG	3 cut(s)	296, 887, 1091
MspA1I	CMGCKG	3 cut(s)	614, 665, 1121
MspI	CCGG	4 cut(s)	522, 537, 543, 894
MspR9I	CCNGG	3 cut(s)	619, 689, 937
Mva1269I	GAATGC	2 cut(s)	593, 1347
MvaI	CCWGG	3 cut(s)	619, 689, 937
MvnI	CGCG	4 cut(s)	216, 252, 330, 1121
MwoI	GCNNNNNNNGC	8 cut(s)	50, 260, 263, 309, 498, 527, 562, 662
NaeI	GCCGGC	2 cut(s)	523, 544
NarI	GGCGCC	2 cut(s)	540, 546
NcoI	CCATGG	1 cut(s)	297
NdeII	GATC	5 cut(s)	789, 1290, 1369, 1375, 1387
NgoMIV	GCCGGC	2 cut(s)	521, 542
NlaIII	CATG	9 cut(s)	133, 153, 301, 481, 565, 589, 1090, 1288, 1321
NlaIV	GGNNCC	8 cut(s)	277, 303, 304, 379, 541, 547, 898, 1143
NmeAIII	GCCGAG	4 cut(s)	354, 966, 1029, 1079
NmuCI	GTSAC	2 cut(s)	1161, 1311
NspI	RCATGY	2 cut(s)	133, 481
PagI	TCATGA	1 cut(s)	1284
PcsI	WCGNNNNNNNCGW	1 cut(s)	447
PctI	GAATGC	2 cut(s)	593, 1347
PdiI	GCCGGC	2 cut(s)	523, 544
PdmI	GAANNNNTTC	1 cut(s)	27
PfeI	GAWTC	1 cut(s)	1281
PkrI	GCNGC	7 cut(s)	256, 265, 476, 491, 616, 1117, 1120
PleI	GAGTC	1 cut(s)	1119
PluTI	GGCGCC	2 cut(s)	543, 549
PpsI	GAGTC	1 cut(s)	1119
Psp124BI	GAGCTC	1 cut(s)	1076
Psp6I	CCWGG	3 cut(s)	617, 687, 935
PspFI	CCCAGC	3 cut(s)	525, 552, 1221
PspGI	CCWGG	3 cut(s)	617, 687, 935
PspN4I	GGNNCC	8 cut(s)	277, 303, 304, 379, 541, 547, 898, 1143
PspOMI	GGGCCC	1 cut(s)	301
PspPI	GGNCC	6 cut(s)	301, 302, 323, 528, 673, 1352
PsuI	RGATCY	2 cut(s)	789, 1387
PvuII	CAGCTG	1 cut(s)	665
RsaI	GTAC	5 cut(s)	669, 718, 857, 1300, 1322
RsaNI	GTAC	5 cut(s)	668, 717, 856, 1299, 1321
RseI	CAYNNNNRTG	3 cut(s)	296, 887, 1091
SacI	GAGCTC	1 cut(s)	1076
SacII	CCGCGG	1 cut(s)	1122
SaqAI	TTAA	1 cut(s)	1059
SatI	GCNGC	7 cut(s)	255, 264, 475, 490, 615, 1116, 1119
Sau3AI	GATC	5 cut(s)	789, 1290, 1369, 1375, 1387
Sau96I	GGNCC	6 cut(s)	301, 302, 323, 528, 673, 1352
ScaI	AGTACT	1 cut(s)	857
SchI	GAGTC	1 cut(s)	1119
ScrFI	CCNGG	3 cut(s)	619, 689, 937
SduI	GDGCHC	3 cut(s)	305, 651, 1076
SfaNI	GCATC	1 cut(s)	128
SfcI	CTRYAG	1 cut(s)	778
SfoI	GGCGCC	2 cut(s)	541, 547
Sfr303I	CCGCGG	1 cut(s)	1122
SgrAI	CRCCGGYG	2 cut(s)	536, 542
SgrBI	CCGCGG	1 cut(s)	1122
SinI	GGWCC	1 cut(s)	673
SmiMI	CAYNNNNRTG	3 cut(s)	296, 887, 1091
Sse9I	AATT	1 cut(s)	98
SspDI	GGCGCC	2 cut(s)	539, 545
SspMI	CTAG	2 cut(s)	320, 1166
SstI	GAGCTC	1 cut(s)	1076
StyD4I	CCNGG	3 cut(s)	617, 687, 935
StyI	CCWWGG	2 cut(s)	297, 319
TaaI	ACNGT	3 cut(s)	627, 1024, 1311
TaiI	ACGT	2 cut(s)	718, 1133
TaqI	TCGA	4 cut(s)	734, 750, 1253, 1374
TasI	AATT	1 cut(s)	98
TatI	WGTACW	4 cut(s)	667, 855, 1298, 1320
TauI	GCSGC	4 cut(s)	257, 477, 1118, 1121
TfiI	GAWTC	1 cut(s)	1281
Tru1I	TTAA	1 cut(s)	1059
Tru9I	TTAA	1 cut(s)	1059
TscAI	CASTG	2 cut(s)	841, 1314
TseFI	GTSAC	2 cut(s)	1161, 1311
TseI	GCWGC	3 cut(s)	263, 489, 614
Tsp45I	GTSAC	2 cut(s)	1161, 1311
TspDTI	ATGAA	4 cut(s)	24, 93, 259, 397
TspRI	CASTG	2 cut(s)	841, 1314
VpaK11BI	GGWCC	1 cut(s)	673
XapI	RAATTY	1 cut(s)	98
XceI	RCATGY	2 cut(s)	133, 481
XmaJI	CCTAGG	1 cut(s)	319
XmnI	GAANNNNTTC	1 cut(s)	27
XspI	CTAG	2 cut(s)	320, 1166
ZrmI	AGTACT	1 cut(s)	857

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.