Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0005591

Physical Location & Seq

Genomic Coordinates Reverse (-)

47421631 .. 47423768

Gene Structure 2138 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G022180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1305 bp

ATGGAAAGCTTTGGTGGCTCTGGATTTTCTGCTGGAGGATGCATTGTAAGGAAGAAAAGGAGTGTTGTATCGCATAAACCTCGCTTGGATCCACTCACATTTTCTGAGAGGTCTAATATCTTGCTACGAATTGTACATCCCTTTGGCAAAGAGAGCCTTGATAAGAATCATGATAGTAAGGAGAACGGAGTGCATTCTGATGGACTGCGAAGTGAGAAAAGGCCGAAGAAATTAAAGCTTAAGCTTCGAGGTGTTACTCATACAATCCAAACCACGTGTACAGTTGATTCTAGTCTTGGTGGTGGCTCTTCCATACCTAAATCCTCATGCCCTTCCGATGTCTTCATACCCCAACCGAAGCCACTTCTCCAGGATGGTGAACCAGCTCGTAAGAGCAAGAGGATTCCTAGGAGACGTGTCTTGGATGTGGGACTTAGTGAAGATGGTAATGAAGATGGTGAAATTCGATTCCTTGAAAGACTAAATGCTTCTAAGGTTGCTGTTTCAAAGAGCAATCAAATGGATGGCGATCATGGAGGTGATATTGGAAACTGTAAATTACCAAGGCTGCGGAAACATGGTGGAATAAAGTCAAGATCAGAAAAAATGTACGAGGATAAAGACTATTTGGGAGAAGAAGAAGAACTGACATCTGACGATGAACTTCAATCTAATGGGAAGACACTGAAAAGGGGATCTCTTAGTTTACTTCTGGAAGGGCCGCAAAAGTCCACACCCACCACACGTAACCGGGCCCTTCAGTCTGGGATAGATATCTTAACTGGTTCTGGTTCAAGCGTTGTTAACCCTGCAAATCTGTTGCTTCCTGCCCCATCTAGAAAAAAGAAGGAGAAAATCTCTGAAGTGGATAAGCAATCAAAGAAAGCCGAGGCTGCACAGAGACGTAAAATACAAACAGAGAAGATAGCTAGAGAAGCTGAGGCTGAGGCAATCAGAAAGATACTTGGTCAAGATTCGAAGAAGAAGAAGAGGGAAGAAGAACTGAAGCAGAAGAGGGATGAATTGTCACAGGGGAGGAATGGCAGTGCTGTCACACTGGCACCAAACACTGTCCGATGGGTCAATGGTCCTAATGGAACGGTCGTTACATTCTCTGATGATATTGGTCTGCCGAACATTTTCAGTCCAGTGCCCTGCAGCTACCCTCCCCCACGTGAAAAATGTGCTGGCCCAAATTGTACAAATGCATACAAGTATCGGGATTCTAAGTCCAAGCTCCCCCTTTGCAGTCTCCACTGCTACAGGGCATTGCACGGAAAGGCGCAGCCTCTAATTGCTTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PAPA-1 zf-HIT

Protein Analysis

434

Amino Acids

47.69

Weight (kDa)

9.64

Isoelectric Point (pI)

55.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAPA-1	PF04795	291 - 374	3.8e-19	PAPA-1-like conserved region
zf-HIT	PF04438	391 - 422	1.9e-06	HIT zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005591)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06660
fragaria_vesca	FvH4_4g19561 FvH4_4g19561 FvH4_4g19561 FvH4_4g19561 FvH4_4g19561
malus_domestica	MD13G1155600.v1.1 MD16G1155300.v1.1
prunus_persica	Prupe.1G118900_v2.0.a1 Prupe.1G118900_v2.0.a1 Prupe.1G118900_v2.0.a1 Prupe.1G118900_v2.0.a1 Prupe.1G118900_v2.0.a1 Prupe.1G118900_v2.0.a1 Prupe.1G118900_v2.0.a1
pyrus_communis	pycom13g13260
rosa_chinensis	RchiOBHm_Chr4g0424821
rosa_laevigata	RLG00000007419
rosa_multiflora	Rmu_sc0003608.1_g000022 Rmu_sc0007418.1_g000001
rosa_roxburghii	Rroxscaffold_5G00367040
rosa_rugosa	Rorug04G0199300
rosa_samantha	Rh4AG257100 Rh4BG262400 Rh4CG273400 Rh4DG258200
rosa_wichuraiana	Rw4G022180 Rw4G024040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1060
AciI	CCGC	2 cut(s)	571, 722
AclWI	GGATC	3 cut(s)	83, 96, 703
AcsI	RAATTY	1 cut(s)	462
AcuI	CTGAAG	3 cut(s)	743, 882, 1025
AcvI	CACGTG	2 cut(s)	276, 1175
AfaI	GTAC	4 cut(s)	135, 280, 611, 1201
AflII	CTTAAG	1 cut(s)	239
AflIII	ACRYGT	3 cut(s)	275, 415, 743
AgsI	TTSAA	4 cut(s)	476, 507, 668, 795
AjiI	CACGTC	1 cut(s)	416
AjnI	CCWGG	1 cut(s)	369
AluBI	AGCT	8 cut(s)	9, 238, 244, 386, 929, 938, 1161, 1237
AluI	AGCT	8 cut(s)	9, 238, 244, 386, 929, 938, 1161, 1237
Alw26I	GTCTC	3 cut(s)	406, 895, 1256
AlwI	GGATC	3 cut(s)	83, 96, 703
AoxI	GGCC	4 cut(s)	221, 719, 753, 1189
ApaI	GGGCCC	1 cut(s)	757
ApeKI	GCWGC	4 cut(s)	568, 893, 1158, 1285
ApoI	RAATTY	1 cut(s)	462
Asp700I	GAANNNNTTC	1 cut(s)	1139
AspA2I	CCTAGG	1 cut(s)	407
AspLEI	GCGC	1 cut(s)	1285
AspS9I	GGNCC	5 cut(s)	719, 753, 754, 1088, 1190
AsuC2I	CCSGG	1 cut(s)	752
AsuHPI	GGTGA	3 cut(s)	389, 470, 551
AsuII	TTCGAA	1 cut(s)	977
AvaII	GGWCC	1 cut(s)	1088
AvrII	CCTAGG	1 cut(s)	407
BaeGI	GKGCMC	2 cut(s)	757, 1155
BamHI	GGATCC	1 cut(s)	88
BanI	GGYRCC	1 cut(s)	1060
BanII	GRGCYC	1 cut(s)	757
BbrPI	CACGTG	2 cut(s)	276, 1175
BbsI	GAAGAC	2 cut(s)	334, 686
BbvCI	CCTCAGC	2 cut(s)	939, 945
BbvI	GCAGC	4 cut(s)	555, 880, 1170, 1297
BccI	CCATC	7 cut(s)	194, 368, 437, 449, 518, 841, 1071
BcgI	CGANNNNNNTGC	2 cut(s)	62, 96
BciT130I	CCWGG	1 cut(s)	371
BcnI	CCSGG	1 cut(s)	752
BcoDI	GTCTC	3 cut(s)	406, 895, 1256
BfaI	CTAG	4 cut(s)	291, 408, 837, 930
BfmI	CTRYAG	2 cut(s)	1156, 1261
BfrI	CTTAAG	1 cut(s)	239
BisI	GCNGC	5 cut(s)	569, 722, 894, 1159, 1286
BlnI	CCTAGG	1 cut(s)	407
BlsI	GCNGC	5 cut(s)	570, 723, 895, 1160, 1287
Bme1390I	CCNGG	2 cut(s)	371, 752
Bme18I	GGWCC	1 cut(s)	1088
BmgBI	CACGTC	1 cut(s)	416
BmgT120I	GGNCC	5 cut(s)	719, 753, 754, 1088, 1190
BmiI	GGNNCC	3 cut(s)	90, 755, 1062
BmrFI	CCNGG	2 cut(s)	371, 752
BmsI	GCATC	1 cut(s)	29
BpiI	GAAGAC	2 cut(s)	334, 686
BplI	GAGNNNNNCTC	2 cut(s)	842, 874
BpmI	CTGGAG	2 cut(s)	54, 353
Bpu10I	CCTNAGC	2 cut(s)	939, 945
Bpu14I	TTCGAA	1 cut(s)	977
BpuMI	CCSGG	1 cut(s)	752
BsaAI	YACGTR	3 cut(s)	276, 746, 1175
BsaBI	GATNNNNATC	3 cut(s)	165, 528, 773
BsaJI	CCNNGG	3 cut(s)	407, 563, 888
Bse1I	ACTGG	3 cut(s)	787, 1062, 1148
Bse3DI	GCAATG	1 cut(s)	1268
Bse8I	GATNNNNATC	3 cut(s)	165, 528, 773
BseBI	CCWGG	1 cut(s)	371
BseDI	CCNNGG	3 cut(s)	407, 563, 888
BseGI	GGATG	6 cut(s)	44, 136, 379, 430, 529, 1024
BseJI	GATNNNNATC	3 cut(s)	165, 528, 773
BseMI	GCAATG	1 cut(s)	1268
BseMII	CTCAG	3 cut(s)	96, 930, 936
BseNI	ACTGG	3 cut(s)	787, 1062, 1148
BseSI	GKGCMC	2 cut(s)	757, 1155
BseXI	GCAGC	4 cut(s)	555, 880, 1170, 1297
BsgI	GTGCAG	1 cut(s)	879
Bsh1285I	CGRYCG	1 cut(s)	1104
BshFI	GGCC	4 cut(s)	223, 721, 755, 1191
BshNI	GGYRCC	1 cut(s)	1060
BsiEI	CGRYCG	1 cut(s)	1104
BsiSI	CCGG	1 cut(s)	751
BslFI	GGGAC	1 cut(s)	444
BsmAI	GTCTC	3 cut(s)	406, 895, 1256
BsmBI	CGTCTC	2 cut(s)	406, 895
BsmFI	GGGAC	1 cut(s)	444
BsmI	GAATGC	1 cut(s)	193
BsnI	GGCC	4 cut(s)	223, 721, 755, 1191
Bsp119I	TTCGAA	1 cut(s)	977
Bsp120I	GGGCCC	1 cut(s)	753
Bsp1286I	GDGCHC	2 cut(s)	757, 1155
Bsp1407I	TGTACA	3 cut(s)	133, 278, 1199
Bsp143I	GATC	4 cut(s)	88, 529, 596, 695
BspACI	CCGC	2 cut(s)	571, 722
BspANI	GGCC	4 cut(s)	223, 721, 755, 1191
BspCNI	CTCAG	3 cut(s)	97, 931, 937
BspHI	TCATGA	1 cut(s)	169
BspLI	GGNNCC	3 cut(s)	90, 755, 1062
BspMAI	CTGCAG	1 cut(s)	1160
BspPI	GGATC	3 cut(s)	83, 96, 703
BspQI	GCTCTTC	1 cut(s)	313
BspT104I	TTCGAA	1 cut(s)	977
BspT107I	GGYRCC	1 cut(s)	1060
BspTI	CTTAAG	1 cut(s)	239
BsrDI	GCAATG	1 cut(s)	1268
BsrGI	TGTACA	3 cut(s)	133, 278, 1199
BsrI	ACTGG	3 cut(s)	787, 1062, 1148
BssECI	CCNNGG	3 cut(s)	407, 563, 888
BssMI	GATC	4 cut(s)	88, 529, 596, 695
BssT1I	CCWWGG	2 cut(s)	407, 563
Bst2UI	CCWGG	1 cut(s)	371
Bst4CI	ACNGT	4 cut(s)	283, 554, 1072, 1102
Bst6I	CTCTTC	3 cut(s)	313, 983, 1007
BstAFI	CTTAAG	1 cut(s)	239
BstAUI	TGTACA	3 cut(s)	133, 278, 1199
BstBAI	YACGTR	3 cut(s)	276, 746, 1175
BstBI	TTCGAA	1 cut(s)	977
BstC8I	GCNNGC	1 cut(s)	1189
BstDEI	CTNAG	7 cut(s)	105, 434, 492, 701, 939, 945, 1227
BstF5I	GGATG	6 cut(s)	44, 136, 379, 430, 529, 1024
BstHHI	GCGC	1 cut(s)	1285
BstKTI	GATC	4 cut(s)	91, 532, 599, 698
BstMAI	GTCTC	3 cut(s)	406, 895, 1256
BstMBI	GATC	4 cut(s)	88, 529, 596, 695
BstMCI	CGRYCG	1 cut(s)	1104
BstMWI	GCNNNNNNNGC	4 cut(s)	15, 153, 893, 935
BstNI	CCWGG	1 cut(s)	371
BstSCI	CCNGG	2 cut(s)	369, 750
BstSFI	CTRYAG	2 cut(s)	1156, 1261
BstSLI	GKGCMC	2 cut(s)	757, 1155
BstV1I	GCAGC	4 cut(s)	555, 880, 1170, 1297
BstV2I	GAAGAC	2 cut(s)	334, 686
BstX2I	RGATCY	2 cut(s)	88, 695
BstYI	RGATCY	2 cut(s)	88, 695
BsuRI	GGCC	4 cut(s)	223, 721, 755, 1191
BtrI	CACGTC	1 cut(s)	416
BtsCI	GGATG	6 cut(s)	44, 136, 379, 430, 529, 1024
BtsI	GCAGTG	2 cut(s)	1051, 1255
BtsIMutI	CAGTG	6 cut(s)	683, 1051, 1055, 1068, 1155, 1255
Cac8I	GCNNGC	1 cut(s)	1189
CciI	TCATGA	1 cut(s)	169
CfoI	GCGC	1 cut(s)	1285
Cfr13I	GGNCC	5 cut(s)	719, 753, 754, 1088, 1190
Csp6I	GTAC	4 cut(s)	134, 279, 610, 1200
CviAII	CATG	4 cut(s)	170, 327, 533, 578
CviQI	GTAC	4 cut(s)	134, 279, 610, 1200
DdeI	CTNAG	7 cut(s)	105, 434, 492, 701, 939, 945, 1227
DpnI	GATC	4 cut(s)	90, 531, 598, 697
DpnII	GATC	4 cut(s)	88, 529, 596, 695
Eam1104I	CTCTTC	3 cut(s)	313, 983, 1007
EarI	CTCTTC	3 cut(s)	313, 983, 1007
Eco130I	CCWWGG	2 cut(s)	407, 563
Eco24I	GRGCYC	1 cut(s)	757
Eco32I	GATATC	1 cut(s)	775
Eco47I	GGWCC	1 cut(s)	1088
Eco57I	CTGAAG	3 cut(s)	743, 882, 1025
Eco72I	CACGTG	2 cut(s)	276, 1175
EcoO109I	RGGNCCY	1 cut(s)	754
EcoRII	CCWGG	1 cut(s)	369
EcoRV	GATATC	1 cut(s)	775
EcoT14I	CCWWGG	2 cut(s)	407, 563
EcoT22I	ATGCAT	2 cut(s)	44, 1210
EcoT38I	GRGCYC	1 cut(s)	757
ErhI	CCWWGG	2 cut(s)	407, 563
Esp3I	CGTCTC	2 cut(s)	406, 895
FaeI	CATG	4 cut(s)	173, 330, 536, 581
FaiI	YATR	9 cut(s)	75, 171, 261, 314, 328, 347, 534, 579, 1210
FalI	AAGNNNNNCTT	2 cut(s)	141, 173
FaqI	GGGAC	1 cut(s)	444
FatI	CATG	4 cut(s)	169, 326, 532, 577
Fnu4HI	GCNGC	5 cut(s)	569, 722, 894, 1159, 1286
FokI	GGATG	6 cut(s)	51, 123, 386, 437, 536, 1031
FriOI	GRGCYC	1 cut(s)	757
Fsp4HI	GCNGC	5 cut(s)	569, 722, 894, 1159, 1286
FspBI	CTAG	4 cut(s)	291, 408, 837, 930
GlaI	GCGC	1 cut(s)	1284
GluI	GCNGC	5 cut(s)	569, 722, 894, 1159, 1286
GsuI	CTGGAG	2 cut(s)	54, 353
HaeIII	GGCC	4 cut(s)	223, 721, 755, 1191
HapII	CCGG	1 cut(s)	751
HhaI	GCGC	1 cut(s)	1285
Hin1II	CATG	4 cut(s)	173, 330, 536, 581
Hin6I	GCGC	1 cut(s)	1283
HinP1I	GCGC	1 cut(s)	1283
HincII	GTYRAC	1 cut(s)	805
HindII	GTYRAC	1 cut(s)	805
HindIII	AAGCTT	3 cut(s)	7, 236, 242
HinfI	GANTC	6 cut(s)	166, 287, 403, 468, 974, 1223
HpaI	GTTAAC	1 cut(s)	805
HpaII	CCGG	1 cut(s)	751
HphI	GGTGA	3 cut(s)	389, 470, 551
Hpy166II	GTNNAC	5 cut(s)	279, 380, 707, 732, 805
Hpy188I	TCNGA	9 cut(s)	106, 199, 337, 601, 655, 862, 956, 1076, 1117
Hpy188III	TCNNGA	7 cut(s)	21, 170, 594, 713, 837, 971, 1220
Hpy8I	GTNNAC	5 cut(s)	279, 380, 707, 732, 805
HpyAV	CCTTC	4 cut(s)	342, 710, 767, 841
HpyCH4III	ACNGT	4 cut(s)	283, 554, 1072, 1102
HpyCH4IV	ACGT	5 cut(s)	275, 415, 745, 904, 1174
HpyCH4V	TGCA	8 cut(s)	42, 193, 812, 896, 1158, 1208, 1248, 1273
HpyF10VI	GCNNNNNNNGC	4 cut(s)	15, 153, 893, 935
HpyF3I	CTNAG	7 cut(s)	105, 434, 492, 701, 939, 945, 1227
HpySE526I	ACGT	5 cut(s)	275, 415, 745, 904, 1174
Hsp92II	CATG	4 cut(s)	173, 330, 536, 581
HspAI	GCGC	1 cut(s)	1283
KspAI	GTTAAC	1 cut(s)	805
Kzo9I	GATC	4 cut(s)	88, 529, 596, 695
LguI	GCTCTTC	1 cut(s)	313
LmnI	GCTCC	1 cut(s)	1242
Lsp1109I	GCAGC	4 cut(s)	555, 880, 1170, 1297
LweI	GCATC	1 cut(s)	29
MaeI	CTAG	4 cut(s)	291, 408, 837, 930
MaeII	ACGT	5 cut(s)	275, 415, 745, 904, 1174
MaeIII	GTNAC	5 cut(s)	253, 746, 1026, 1051, 1105
MalI	GATC	4 cut(s)	90, 531, 598, 697
MboI	GATC	4 cut(s)	88, 529, 596, 695
MflI	RGATCY	2 cut(s)	88, 695
MhlI	GDGCHC	2 cut(s)	757, 1155
MluCI	AATT	7 cut(s)	129, 230, 462, 557, 1022, 1195, 1293
Mph1103I	ATGCAT	2 cut(s)	44, 1210
MroXI	GAANNNNTTC	1 cut(s)	1139
MseI	TTAA	4 cut(s)	233, 240, 779, 804
MslI	CAYNNNNRTG	2 cut(s)	198, 537
MspCI	CTTAAG	1 cut(s)	239
MspI	CCGG	1 cut(s)	751
MspR9I	CCNGG	2 cut(s)	371, 752
Mva1269I	GAATGC	1 cut(s)	193
MvaI	CCWGG	1 cut(s)	371
MwoI	GCNNNNNNNGC	4 cut(s)	15, 153, 893, 935
NciI	CCSGG	1 cut(s)	752
NdeII	GATC	4 cut(s)	88, 529, 596, 695
NlaIII	CATG	4 cut(s)	173, 330, 536, 581
NlaIV	GGNNCC	3 cut(s)	90, 755, 1062
NmeAIII	GCCGAG	1 cut(s)	913
NmuCI	GTSAC	2 cut(s)	1026, 1051
NsiI	ATGCAT	2 cut(s)	44, 1210
NspV	TTCGAA	1 cut(s)	977
PagI	TCATGA	1 cut(s)	169
PciSI	GCTCTTC	1 cut(s)	313
PctI	GAATGC	1 cut(s)	193
PdmI	GAANNNNTTC	1 cut(s)	1139
PfeI	GAWTC	6 cut(s)	166, 287, 403, 468, 974, 1223
PfoI	TCCNGGA	1 cut(s)	369
PkrI	GCNGC	5 cut(s)	570, 723, 895, 1160, 1287
PmaCI	CACGTG	2 cut(s)	276, 1175
PmlI	CACGTG	2 cut(s)	276, 1175
Ppu21I	YACGTR	3 cut(s)	276, 746, 1175
Psp6I	CCWGG	1 cut(s)	369
PspCI	CACGTG	2 cut(s)	276, 1175
PspGI	CCWGG	1 cut(s)	369
PspN4I	GGNNCC	3 cut(s)	90, 755, 1062
PspOMI	GGGCCC	1 cut(s)	753
PspPI	GGNCC	5 cut(s)	719, 753, 754, 1088, 1190
PsrI	GAACNNNNNNTAC	2 cut(s)	1090, 1122
PstI	CTGCAG	1 cut(s)	1160
PsuI	RGATCY	2 cut(s)	88, 695
RsaI	GTAC	4 cut(s)	135, 280, 611, 1201
RsaNI	GTAC	4 cut(s)	134, 279, 610, 1200
RseI	CAYNNNNRTG	2 cut(s)	198, 537
SapI	GCTCTTC	1 cut(s)	313
SaqAI	TTAA	4 cut(s)	233, 240, 779, 804
SatI	GCNGC	5 cut(s)	569, 722, 894, 1159, 1286
Sau3AI	GATC	4 cut(s)	88, 529, 596, 695
Sau96I	GGNCC	5 cut(s)	719, 753, 754, 1088, 1190
ScrFI	CCNGG	2 cut(s)	371, 752
SduI	GDGCHC	2 cut(s)	757, 1155
SfaNI	GCATC	1 cut(s)	29
SfcI	CTRYAG	2 cut(s)	1156, 1261
SfuI	TTCGAA	1 cut(s)	977
SinI	GGWCC	1 cut(s)	1088
SmiMI	CAYNNNNRTG	2 cut(s)	198, 537
SmlI	CTYRAG	1 cut(s)	239
SmoI	CTYRAG	1 cut(s)	239
Sse9I	AATT	7 cut(s)	129, 230, 462, 557, 1022, 1195, 1293
SsiI	CCGC	2 cut(s)	571, 722
SspMI	CTAG	4 cut(s)	291, 408, 837, 930
StyD4I	CCNGG	2 cut(s)	369, 750
StyI	CCWWGG	2 cut(s)	407, 563
TaaI	ACNGT	4 cut(s)	283, 554, 1072, 1102
TaiI	ACGT	5 cut(s)	278, 418, 748, 907, 1177
TaqI	TCGA	3 cut(s)	247, 466, 977
TasI	AATT	7 cut(s)	129, 230, 462, 557, 1022, 1195, 1293
TatI	WGTACW	3 cut(s)	133, 278, 1199
TauI	GCSGC	1 cut(s)	724
TfiI	GAWTC	6 cut(s)	166, 287, 403, 468, 974, 1223
Tru1I	TTAA	4 cut(s)	233, 240, 779, 804
Tru9I	TTAA	4 cut(s)	233, 240, 779, 804
TscAI	CASTG	6 cut(s)	690, 1051, 1062, 1075, 1155, 1262
TseFI	GTSAC	2 cut(s)	1026, 1051
TseI	GCWGC	4 cut(s)	568, 893, 1158, 1285
Tsp45I	GTSAC	2 cut(s)	1026, 1051
TspDTI	ATGAA	4 cut(s)	334, 465, 675, 1035
TspGWI	ACGGA	2 cut(s)	201, 1290
TspRI	CASTG	6 cut(s)	690, 1051, 1062, 1075, 1155, 1262
Vha464I	CTTAAG	1 cut(s)	239
VpaK11BI	GGWCC	1 cut(s)	1088
XapI	RAATTY	1 cut(s)	462
XbaI	TCTAGA	1 cut(s)	836
XmaJI	CCTAGG	1 cut(s)	407
XmnI	GAANNNNTTC	1 cut(s)	1139
XspI	CTAG	4 cut(s)	291, 408, 837, 930
Zsp2I	ATGCAT	2 cut(s)	44, 1210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.