Rw4G025070

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014720
Physical Location & Seq
Forward (+)
51059046 .. 51060284
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025070.1

Sequence Viewer

Length: 612 bp
ATGATCAGCCGTAACTTAGAGCAGAAAAGAACAGGAAGCGAAAATGGTAATCGGGAGCATCAATTGAAGGAGCTGATGGGGAAGAGGAGCAGACTTTCTGCTGAAGGAGTCGCAACAGAAGATTATGTTCGTGTCAAGGCAAGGAGGGGTGAAGCAACCGACAGTCACAGTCTAGCGGAAAGGGCAAGAAGAGCGCAAATCCGAATGAGAATGAAACTCTTGCAATCACTTGTTCCTGGATGCGATAAGATAACTGGAAAGGCTCATATACTGGATGAGATTATCAAATATGTTCAATTGCTACAGAATCAAGTAGAGTCCCTTGCGGCAAAGCTTGCTTTTGTGGATCTCATGCTATACGACTGTGAGCCGAATCCAAGCGGAAATGCATGTGTTCCTGAGGGGCTGTTGCCGTCTGTGCCAGAGTCTAGCTCTATCCCGTTCATGTCTTTTGCAGACGGTGGGGTAAGCCATTCTGCTTCCCTTTTGCTCAAAGAGGATGATCAGATAGCTACTCTAATCCCTCAGTATGGTAGAGATTTGTTATTGGACCTGGAAGAGCAGCCTGAAGCTATTAGACAACTTATGCACCTTCTAAGCTCAGCCAATTAG

Protein Analysis

203

Amino Acids

22.54

Weight (kDa)

5.99

Isoelectric Point (pI)

57.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 55 - 102 6.6e-08 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 60 - 107 1.4e-06 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014720)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g22890 FvH4_4g22890
malus_domestica MD13G1128900.v1.1
prunus_persica Prupe.1G217700_v2.0.a1 Prupe.1G217700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0429161
rosa_laevigata RLG00000007093
rosa_multiflora Rmu_sc0000846.1_g000009
rosa_roxburghii Rroxscaffold_5G00370810
rosa_rugosa Rorug04G0233300 Rorug04G0233400 Rorug04G0233500
rosa_samantha Rh4AG289300 Rh4BG295300 Rh4CG310900 Rh4DG292300
rosa_wichuraiana Rw4G025070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 176, 326, 381
AclWI GGATC 1 cut(s) 354
AcuI CTGAAG 2 cut(s) 123, 588
AfiI CCNNNNNNNGG 1 cut(s) 530
AgsI TTSAA 2 cut(s) 67, 296
AjnI CCWGG 2 cut(s) 235, 552
AluBI AGCT 6 cut(s) 73, 334, 432, 512, 572, 600
AluI AGCT 6 cut(s) 73, 334, 432, 512, 572, 600
AlwI GGATC 1 cut(s) 354
ApeKI GCWGC 1 cut(s) 562
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 550
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 1 cut(s) 550
AxyI CCTNAGG 1 cut(s) 399
BbvI GCAGC 1 cut(s) 574
BccI CCATC 1 cut(s) 70
BceAI ACGGC 1 cut(s) 397
BciT130I CCWGG 2 cut(s) 237, 554
BclI TGATCA 2 cut(s) 3, 502
BfaI CTAG 2 cut(s) 173, 429
BfmI CTRYAG 1 cut(s) 302
BisI GCNGC 2 cut(s) 327, 563
BlpI GCTNAGC 1 cut(s) 601
BlsI GCNGC 2 cut(s) 328, 564
Bme1390I CCNGG 2 cut(s) 237, 554
Bme18I GGWCC 1 cut(s) 550
BmgT120I GGNCC 1 cut(s) 550
BmrFI CCNGG 2 cut(s) 237, 554
BmsI GCATC 2 cut(s) 67, 230
BplI GAGNNNNNCTC 2 cut(s) 416, 448
Bpu1102I GCTNAGC 1 cut(s) 601
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse1I ACTGG 2 cut(s) 259, 276
Bse21I CCTNAGG 1 cut(s) 399
BseBI CCWGG 2 cut(s) 237, 554
BseGI GGATG 3 cut(s) 245, 280, 505
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMII CTCAG 2 cut(s) 390, 539
BseNI ACTGG 2 cut(s) 259, 276
BseRI GAGGAG 1 cut(s) 100
BseXI GCAGC 1 cut(s) 574
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 1 cut(s) 530
BsmFI GGGAC 1 cut(s) 304
Bsp143I GATC 3 cut(s) 3, 346, 502
Bsp1720I GCTNAGC 1 cut(s) 601
BspACI CCGC 3 cut(s) 176, 326, 381
BspCNI CTCAG 2 cut(s) 391, 538
BspPI GGATC 1 cut(s) 354
BspQI GCTCTTC 2 cut(s) 184, 552
BsrI ACTGG 2 cut(s) 259, 276
BssMI GATC 3 cut(s) 3, 346, 502
Bst2UI CCWGG 2 cut(s) 237, 554
Bst4CI ACNGT 4 cut(s) 164, 170, 365, 461
Bst6I CTCTTC 3 cut(s) 77, 184, 552
BstAPI GCANNNNNTGC 1 cut(s) 335
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 5 cut(s) 16, 399, 525, 596, 601
BstF5I GGATG 3 cut(s) 245, 280, 505
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 3 cut(s) 6, 349, 505
BstMBI GATC 3 cut(s) 3, 346, 502
BstMWI GCNNNNNNNGC 4 cut(s) 182, 191, 335, 418
BstNI CCWGG 2 cut(s) 237, 554
BstNSI RCATGY 1 cut(s) 393
BstSCI CCNGG 2 cut(s) 235, 552
BstSFI CTRYAG 1 cut(s) 302
BstV1I GCAGC 1 cut(s) 574
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu36I CCTNAGG 1 cut(s) 399
BtsCI GGATG 3 cut(s) 245, 280, 505
Cac8I GCNNGC 1 cut(s) 336
CfoI GCGC 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 550
CviAII CATG 3 cut(s) 352, 390, 445
DdeI CTNAG 5 cut(s) 16, 399, 525, 596, 601
DpnI GATC 3 cut(s) 5, 348, 504
DpnII GATC 3 cut(s) 3, 346, 502
Eam1104I CTCTTC 3 cut(s) 77, 184, 552
EarI CTCTTC 3 cut(s) 77, 184, 552
Eco47I GGWCC 1 cut(s) 550
Eco57I CTGAAG 2 cut(s) 123, 588
Eco81I CCTNAGG 1 cut(s) 399
EcoRII CCWGG 2 cut(s) 235, 552
EcoT22I ATGCAT 1 cut(s) 391
FaeI CATG 3 cut(s) 355, 393, 448
FaqI GGGAC 1 cut(s) 304
FatI CATG 3 cut(s) 351, 389, 444
FbaI TGATCA 2 cut(s) 3, 502
Fnu4HI GCNGC 2 cut(s) 327, 563
FokI GGATG 3 cut(s) 252, 287, 512
Fsp4HI GCNGC 2 cut(s) 327, 563
FspBI CTAG 2 cut(s) 173, 429
GlaI GCGC 1 cut(s) 195
GluI GCNGC 2 cut(s) 327, 563
HhaI GCGC 1 cut(s) 196
Hin1II CATG 3 cut(s) 355, 393, 448
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 5 cut(s) 108, 307, 317, 373, 425
HphI GGTGA 1 cut(s) 161
Hpy188I TCNGA 2 cut(s) 203, 507
Hpy188III TCNNGA 2 cut(s) 53, 398
HpyAV CCTTC 3 cut(s) 61, 98, 602
HpyCH4III ACNGT 4 cut(s) 164, 170, 365, 461
HpyCH4V TGCA 4 cut(s) 223, 389, 455, 589
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 191, 335, 418
HpyF3I CTNAG 5 cut(s) 16, 399, 525, 596, 601
Hsp92II CATG 3 cut(s) 355, 393, 448
HspAI GCGC 1 cut(s) 194
Ksp22I TGATCA 2 cut(s) 3, 502
Kzo9I GATC 3 cut(s) 3, 346, 502
LguI GCTCTTC 2 cut(s) 184, 552
LmnI GCTCC 3 cut(s) 55, 70, 87
Lsp1109I GCAGC 1 cut(s) 574
LweI GCATC 2 cut(s) 67, 230
MaeI CTAG 2 cut(s) 173, 429
MaeIII GTNAC 2 cut(s) 11, 164
MalI GATC 3 cut(s) 5, 348, 504
MboI GATC 3 cut(s) 3, 346, 502
MboII GAAGA 4 cut(s) 94, 131, 201, 569
MfeI CAATTG 2 cut(s) 62, 296
MflI RGATCY 1 cut(s) 346
MluCI AATT 3 cut(s) 62, 296, 607
MlyI GAGTC 3 cut(s) 117, 326, 434
MnlI CCTC 5 cut(s) 78, 138, 394, 490, 534
Mph1103I ATGCAT 1 cut(s) 391
MspR9I CCNGG 2 cut(s) 237, 554
MunI CAATTG 2 cut(s) 62, 296
MvaI CCWGG 2 cut(s) 237, 554
MwoI GCNNNNNNNGC 4 cut(s) 182, 191, 335, 418
NdeII GATC 3 cut(s) 3, 346, 502
NlaIII CATG 3 cut(s) 355, 393, 448
NmuCI GTSAC 1 cut(s) 164
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 1 cut(s) 393
PciSI GCTCTTC 2 cut(s) 184, 552
PfeI GAWTC 2 cut(s) 307, 373
PfoI TCCNGGA 1 cut(s) 235
PkrI GCNGC 2 cut(s) 328, 564
PleI GAGTC 3 cut(s) 116, 325, 433
PpsI GAGTC 3 cut(s) 116, 325, 433
Psp6I CCWGG 2 cut(s) 235, 552
PspGI CCWGG 2 cut(s) 235, 552
PspPI GGNCC 1 cut(s) 550
PsuI RGATCY 1 cut(s) 346
SapI GCTCTTC 2 cut(s) 184, 552
SatI GCNGC 2 cut(s) 327, 563
Sau3AI GATC 3 cut(s) 3, 346, 502
Sau96I GGNCC 1 cut(s) 550
SchI GAGTC 3 cut(s) 117, 326, 434
ScrFI CCNGG 2 cut(s) 237, 554
SetI ASST 8 cut(s) 75, 336, 434, 514, 555, 574, 594, 602
SfaNI GCATC 2 cut(s) 67, 230
SfcI CTRYAG 1 cut(s) 302
SinI GGWCC 1 cut(s) 550
Sse9I AATT 3 cut(s) 62, 296, 607
SsiI CCGC 3 cut(s) 176, 326, 381
SspMI CTAG 2 cut(s) 173, 429
StyD4I CCNGG 2 cut(s) 235, 552
TaaI ACNGT 4 cut(s) 164, 170, 365, 461
TasI AATT 3 cut(s) 62, 296, 607
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 2 cut(s) 307, 373
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 1 cut(s) 562
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 2 cut(s) 227, 433
VpaK11BI GGWCC 1 cut(s) 550
XceI RCATGY 1 cut(s) 393
XspI CTAG 2 cut(s) 173, 429
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.