Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0006236

Physical Location & Seq

Genomic Coordinates Reverse (-)

61717726 .. 61722113

Gene Structure 4388 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G034730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1176 bp

ATGACCAAGTCCAAGAACTTCCTCAACAATCTCATCAAAACCTTCGGGTTCGGCTCAAGCAAACATGGCCATAATGAAGAGGACTTGGAGAAAATTGCTCAACAGGAACAGAAGGTTTTCACTTTTGAGACTTTGGTGGCTGCTACCAAGGATTTTCACCCCGATAATAAGCTCGGACAAGGCGGATTTGGGCCGGTTTACAAGGGGAGATTGCCTGATGGTAGAGCTATTGCAGTGAAGAAGCTGTCACAAAGTTCAAGTCAAGGAAAAAAAGAGTTCATGAACGAAGCCAAGTTGTTGGCTCGCGTGCAACACCGCAATGTTGTGAATTTATTGGGCTATTGTGCTCATGGGGGAGAGAAACTACTTGTATATGAATATGTCGTACATGAGAGCGTTGACAAGCTTCTCTTCAAACCAGATAGACGACCGGAGCTTGACTGGAAGCGGAGGTATGATATAATATGTGGCGTTGCAAAAGGTTTGCTTTATCTTCATGAAGACTCGCACAATCGTATTATACACAGAGACATCAAGGCCAGCAACATTCTGCTTGATGATAAATGGGTCCCCAAGATTGCTGATTTTGGCATGGCTCGTCTCTTTCCTGAAGATCAAACGCACGTTAATACGCGTGTTGCTGGGACCAATGGGTATATGGCTCCAGAGTATGTCATGCATGGACATTTATCGGAGAAGGCCGATGTTTTTAGCTTTGGTGTGTTGGTTTTGGAGCTTATCAGCGGCCAAAGGAATTCGTCATTCAACTTAAATGTTGAAGCGCAGAGCCTTCTCGATTGGGCATACAAGCTGTACAAGAAGGGGAGAAGCTTGGAGATGATGGACCCAACATTAGCACCATCCGCGGTCACTGATCAAGTAGCAATGTGTATCCAGATAGGGTTATTATGCATACAAGGCGACCCCCAGATCAGACCCACCATGCATCGCGTGGTCGTGATCCTATCAAAGAAACCGAGCAATCTTGAAGAGCCAACTAGACCTGGAATACCAGGATCAAGATACAGAAGAGCTCACAGACCAGCATCATCCTCCACTGCTGGGACTTCTGGTGAATCAAATTCTCATACATTCGGATCGAGCTTTACCGCAACTGGAACCAGTTCAGCAACCCGAGTTGTACAAGACCGGGGGAAACGTCCAATGGAGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

391

Amino Acids

43.65

Weight (kDa)

9.34

Isoelectric Point (pI)

38.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	53 - 318	3.8e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	55 - 320	3.7e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006236)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G70740 AT1G70740
fragaria_vesca	FvH4_4g34050 FvH4_4g34050 FvH4_4g34050 FvH4_4g34050 FvH4_4g34050 FvH4_4g34050 FvH4_4g34050
malus_domestica	MD13G1021200.v1.1 MD16G1022500.v1.1
prunus_persica	Prupe.1G330500_v2.0.a1 Prupe.1G330500_v2.0.a1 Prupe.1G330500_v2.0.a1 Prupe.1G330500_v2.0.a1
pyrus_communis	pycom13g02010 pycom16g01980
rosa_chinensis	RchiOBHm_Chr4g0443541
rosa_laevigata	RLG00000005918
rosa_multiflora	Rmu_sc0000648.1_g000006 Rmu_sc0001118.1_g000023
rosa_roxburghii	Rroxscaffold_5G00384090
rosa_samantha	Rh4AG402800 Rh4BG414500 Rh4CG430100 Rh4DG410000
rosa_wichuraiana	Rw4G034730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	4 cut(s)	306, 634, 866, 951
AciI	CCGC	7 cut(s)	183, 316, 448, 744, 864, 866, 1110
AclWI	GGATC	3 cut(s)	955, 1024, 1105
AcoI	YGGCCR	2 cut(s)	67, 745
AcsI	RAATTY	3 cut(s)	328, 754, 1081
AcuI	CTGAAG	1 cut(s)	630
AfaI	GTAC	3 cut(s)	387, 815, 1143
AfiI	CCNNNNNNNGG	1 cut(s)	1062
AflIII	ACRYGT	1 cut(s)	632
AgsI	TTSAA	5 cut(s)	258, 415, 766, 779, 989
AjnI	CCWGG	2 cut(s)	1003, 1012
Alw21I	GWGCWC	2 cut(s)	349, 1036
Alw26I	GTCTC	3 cut(s)	122, 522, 605
AlwI	GGATC	3 cut(s)	955, 1024, 1105
Ama87I	CYCGRG	1 cut(s)	1134
AoxI	GGCC	5 cut(s)	67, 191, 537, 699, 745
ApeKI	GCWGC	1 cut(s)	140
ApoI	RAATTY	3 cut(s)	328, 754, 1081
ArsI	GACNNNNNNTTYG	2 cut(s)	244, 276
Asp700I	GAANNNNTTC	2 cut(s)	116, 1123
AspLEI	GCGC	1 cut(s)	784
AspS9I	GGNCC	4 cut(s)	191, 568, 645, 844
AsuC2I	CCSGG	1 cut(s)	1151
AsuHPI	GGTGA	2 cut(s)	149, 1085
AvaI	CYCGRG	1 cut(s)	1134
AvaII	GGWCC	3 cut(s)	568, 645, 844
BalI	TGGCCA	1 cut(s)	69
BanII	GRGCYC	1 cut(s)	1036
BbsI	GAAGAC	1 cut(s)	507
Bbv12I	GWGCWC	2 cut(s)	349, 1036
BbvI	GCAGC	1 cut(s)	127
BccI	CCATC	3 cut(s)	212, 835, 868
BciT130I	CCWGG	2 cut(s)	1005, 1014
BciVI	GTATCC	1 cut(s)	902
BclI	TGATCA	1 cut(s)	874
BcnI	CCSGG	1 cut(s)	1151
BcoDI	GTCTC	3 cut(s)	122, 522, 605
BfaI	CTAG	1 cut(s)	999
BfuI	GTATCC	1 cut(s)	902
BisI	GCNGC	2 cut(s)	141, 745
BlsI	GCNGC	2 cut(s)	142, 746
Bme1390I	CCNGG	3 cut(s)	1005, 1014, 1151
Bme18I	GGWCC	3 cut(s)	568, 645, 844
BmeT110I	CYCGRG	1 cut(s)	1134
BmgT120I	GGNCC	4 cut(s)	191, 568, 645, 844
BmiI	GGNNCC	6 cut(s)	569, 570, 646, 663, 846, 1120
BmrFI	CCNGG	3 cut(s)	1005, 1014, 1151
BmsI	GCATC	2 cut(s)	955, 1055
BpiI	GAAGAC	1 cut(s)	507
BpmI	CTGGAG	1 cut(s)	648
BpuEI	CTTGAG	1 cut(s)	40
BpuMI	CCSGG	1 cut(s)	1151
BsaJI	CCNNGG	3 cut(s)	147, 864, 1150
BsaWI	WCCGGW	1 cut(s)	430
Bsc4I	CCNNNNNNNGG	1 cut(s)	1062
Bse118I	RCCGGY	1 cut(s)	193
Bse1I	ACTGG	3 cut(s)	446, 1120, 1122
Bse3DI	GCAATG	2 cut(s)	325, 891
BseBI	CCWGG	2 cut(s)	1005, 1014
BseDI	CCNNGG	3 cut(s)	147, 864, 1150
BseGI	GGATG	2 cut(s)	860, 1049
BseLI	CCNNNNNNNGG	1 cut(s)	1062
BseMI	GCAATG	2 cut(s)	325, 891
BseNI	ACTGG	3 cut(s)	446, 1120, 1122
BseXI	GCAGC	1 cut(s)	127
BseYI	CCCAGC	2 cut(s)	641, 1061
Bsh1236I	CGCG	4 cut(s)	306, 634, 866, 951
Bsh1285I	CGRYCG	1 cut(s)	431
BshFI	GGCC	5 cut(s)	69, 193, 539, 701, 747
BsiEI	CGRYCG	1 cut(s)	431
BsiHKAI	GWGCWC	2 cut(s)	349, 1036
BsiHKCI	CYCGRG	1 cut(s)	1134
BsiSI	CCGG	3 cut(s)	194, 431, 1150
BslFI	GGGAC	3 cut(s)	554, 658, 1078
BslI	CCNNNNNNNGG	1 cut(s)	1062
BsmAI	GTCTC	3 cut(s)	122, 522, 605
BsmBI	CGTCTC	1 cut(s)	605
BsmFI	GGGAC	3 cut(s)	554, 658, 1078
BsnI	GGCC	5 cut(s)	69, 193, 539, 701, 747
BsoBI	CYCGRG	1 cut(s)	1134
Bsp1286I	GDGCHC	2 cut(s)	349, 1036
Bsp1407I	TGTACA	2 cut(s)	813, 1141
Bsp143I	GATC	6 cut(s)	613, 874, 930, 960, 1016, 1097
BspACI	CCGC	7 cut(s)	183, 316, 448, 744, 864, 866, 1110
BspANI	GGCC	5 cut(s)	69, 193, 539, 701, 747
BspFNI	CGCG	4 cut(s)	306, 634, 866, 951
BspHI	TCATGA	2 cut(s)	279, 496
BspLI	GGNNCC	6 cut(s)	569, 570, 646, 663, 846, 1120
BspPI	GGATC	3 cut(s)	955, 1024, 1105
BspQI	GCTCTTC	2 cut(s)	984, 1024
BsrDI	GCAATG	2 cut(s)	325, 891
BsrFI	RCCGGY	1 cut(s)	193
BsrGI	TGTACA	2 cut(s)	813, 1141
BsrI	ACTGG	3 cut(s)	446, 1120, 1122
BssAI	RCCGGY	1 cut(s)	193
BssECI	CCNNGG	3 cut(s)	147, 864, 1150
BssMI	GATC	6 cut(s)	613, 874, 930, 960, 1016, 1097
BssT1I	CCWWGG	1 cut(s)	147
Bst2UI	CCWGG	2 cut(s)	1005, 1014
Bst6I	CTCTTC	4 cut(s)	72, 416, 984, 1024
BstAUI	TGTACA	2 cut(s)	813, 1141
BstC8I	GCNNGC	3 cut(s)	304, 308, 541
BstDSI	CCRYGG	1 cut(s)	864
BstF5I	GGATG	2 cut(s)	860, 1049
BstFNI	CGCG	4 cut(s)	306, 634, 866, 951
BstHHI	GCGC	1 cut(s)	784
BstKTI	GATC	6 cut(s)	616, 877, 933, 963, 1019, 1100
BstMAI	GTCTC	3 cut(s)	122, 522, 605
BstMBI	GATC	6 cut(s)	613, 874, 930, 960, 1016, 1097
BstMCI	CGRYCG	1 cut(s)	431
BstMWI	GCNNNNNNNGC	3 cut(s)	66, 863, 918
BstNI	CCWGG	2 cut(s)	1005, 1014
BstSCI	CCNGG	3 cut(s)	1003, 1012, 1149
BstUI	CGCG	4 cut(s)	306, 634, 866, 951
BstV1I	GCAGC	1 cut(s)	127
BstV2I	GAAGAC	1 cut(s)	507
BstXI	CCANNNNNNTGG	1 cut(s)	298
BsuI	GTATCC	1 cut(s)	902
BsuRI	GGCC	5 cut(s)	69, 193, 539, 701, 747
BtgI	CCRYGG	1 cut(s)	864
BtgZI	GCGATG	1 cut(s)	932
BtsCI	GGATG	2 cut(s)	860, 1049
BtsI	GCAGTG	2 cut(s)	240, 1056
BtsIMutI	CAGTG	3 cut(s)	240, 870, 1056
Cac8I	GCNNGC	3 cut(s)	304, 308, 541
CciI	TCATGA	2 cut(s)	279, 496
CfoI	GCGC	1 cut(s)	784
Cfr10I	RCCGGY	1 cut(s)	193
Cfr13I	GGNCC	4 cut(s)	191, 568, 645, 844
Cfr42I	CCGCGG	1 cut(s)	867
Csp6I	GTAC	3 cut(s)	386, 814, 1142
CviAII	CATG	9 cut(s)	65, 280, 350, 389, 497, 592, 676, 680, 943
CviQI	GTAC	3 cut(s)	386, 814, 1142
DpnI	GATC	6 cut(s)	615, 876, 932, 962, 1018, 1099
DpnII	GATC	6 cut(s)	613, 874, 930, 960, 1016, 1097
EaeI	YGGCCR	2 cut(s)	67, 745
Eam1104I	CTCTTC	4 cut(s)	72, 416, 984, 1024
EarI	CTCTTC	4 cut(s)	72, 416, 984, 1024
EciI	GGCGGA	1 cut(s)	198
Ecl136II	GAGCTC	1 cut(s)	1034
Eco130I	CCWWGG	1 cut(s)	147
Eco24I	GRGCYC	1 cut(s)	1036
Eco47I	GGWCC	3 cut(s)	568, 645, 844
Eco53kI	GAGCTC	1 cut(s)	1034
Eco57I	CTGAAG	1 cut(s)	630
Eco88I	CYCGRG	1 cut(s)	1134
EcoICRI	GAGCTC	1 cut(s)	1034
EcoO109I	RGGNCCY	1 cut(s)	568
EcoRI	GAATTC	1 cut(s)	754
EcoRII	CCWGG	2 cut(s)	1003, 1012
EcoT14I	CCWWGG	1 cut(s)	147
EcoT22I	ATGCAT	3 cut(s)	681, 914, 948
EcoT38I	GRGCYC	1 cut(s)	1036
ErhI	CCWWGG	1 cut(s)	147
Esp3I	CGTCTC	1 cut(s)	605
FaeI	CATG	9 cut(s)	68, 283, 353, 392, 500, 595, 679, 683, 946
FalI	AAGNNNNNCTT	4 cut(s)	395, 427, 471, 503
FaqI	GGGAC	3 cut(s)	554, 658, 1078
FatI	CATG	9 cut(s)	64, 279, 349, 388, 496, 591, 675, 679, 942
FbaI	TGATCA	1 cut(s)	874
Fnu4HI	GCNGC	2 cut(s)	141, 745
FokI	GGATG	2 cut(s)	847, 1036
FriOI	GRGCYC	1 cut(s)	1036
Fsp4HI	GCNGC	2 cut(s)	141, 745
FspBI	CTAG	1 cut(s)	999
GlaI	GCGC	1 cut(s)	783
GluI	GCNGC	2 cut(s)	141, 745
GsaI	CCCAGC	2 cut(s)	645, 1065
GsuI	CTGGAG	1 cut(s)	648
HaeIII	GGCC	5 cut(s)	69, 193, 539, 701, 747
HapII	CCGG	3 cut(s)	194, 431, 1150
HhaI	GCGC	1 cut(s)	784
Hin1II	CATG	9 cut(s)	68, 283, 353, 392, 500, 595, 679, 683, 946
Hin6I	GCGC	1 cut(s)	782
HinP1I	GCGC	1 cut(s)	782
HincII	GTYRAC	1 cut(s)	400
HindII	GTYRAC	1 cut(s)	400
HindIII	AAGCTT	2 cut(s)	404, 829
HinfI	GANTC	2 cut(s)	503, 1076
HpaII	CCGG	3 cut(s)	194, 431, 1150
HphI	GGTGA	2 cut(s)	149, 1085
Hpy166II	GTNNAC	2 cut(s)	199, 400
Hpy188I	TCNGA	4 cut(s)	176, 694, 935, 1097
Hpy188III	TCNNGA	9 cut(s)	280, 497, 608, 665, 794, 895, 958, 986, 1020
Hpy8I	GTNNAC	2 cut(s)	199, 400
HpyAV	CCTTC	5 cut(s)	52, 106, 691, 800, 814
HpyCH4IV	ACGT	2 cut(s)	624, 1159
HpyCH4V	TGCA	6 cut(s)	233, 310, 476, 679, 912, 946
HpyF10VI	GCNNNNNNNGC	3 cut(s)	66, 863, 918
HpySE526I	ACGT	2 cut(s)	624, 1159
Hsp92II	CATG	9 cut(s)	68, 283, 353, 392, 500, 595, 679, 683, 946
HspAI	GCGC	1 cut(s)	782
KflI	GGGWCCC	1 cut(s)	568
Ksp22I	TGATCA	1 cut(s)	874
KspI	CCGCGG	1 cut(s)	867
Kzo9I	GATC	6 cut(s)	613, 874, 930, 960, 1016, 1097
LguI	GCTCTTC	2 cut(s)	984, 1024
LmnI	GCTCC	3 cut(s)	433, 667, 733
Lsp1109I	GCAGC	1 cut(s)	127
LweI	GCATC	2 cut(s)	955, 1055
MaeI	CTAG	1 cut(s)	999
MaeII	ACGT	2 cut(s)	624, 1159
MaeIII	GTNAC	2 cut(s)	246, 868
MalI	GATC	6 cut(s)	615, 876, 932, 962, 1018, 1099
MboI	GATC	6 cut(s)	613, 874, 930, 960, 1016, 1097
MboII	GAAGA	8 cut(s)	89, 250, 403, 485, 512, 623, 1001, 1041
MhlI	GDGCHC	2 cut(s)	349, 1036
MlsI	TGGCCA	1 cut(s)	69
MluCI	AATT	4 cut(s)	93, 328, 754, 1081
MluI	ACGCGT	1 cut(s)	632
MluNI	TGGCCA	1 cut(s)	69
MlyI	GAGTC	1 cut(s)	497
MnlI	CCTC	4 cut(s)	32, 73, 444, 1063
Mox20I	TGGCCA	1 cut(s)	69
Mph1103I	ATGCAT	3 cut(s)	681, 914, 948
MroXI	GAANNNNTTC	2 cut(s)	116, 1123
MscI	TGGCCA	1 cut(s)	69
MseI	TTAA	2 cut(s)	627, 770
MslI	CAYNNNNRTG	1 cut(s)	318
Msp20I	TGGCCA	1 cut(s)	69
MspA1I	CMGCKG	2 cut(s)	744, 866
MspI	CCGG	3 cut(s)	194, 431, 1150
MspR9I	CCNGG	3 cut(s)	1005, 1014, 1151
MvaI	CCWGG	2 cut(s)	1005, 1014
MvnI	CGCG	4 cut(s)	306, 634, 866, 951
MwoI	GCNNNNNNNGC	3 cut(s)	66, 863, 918
NciI	CCSGG	1 cut(s)	1151
NdeII	GATC	6 cut(s)	613, 874, 930, 960, 1016, 1097
NlaIII	CATG	9 cut(s)	68, 283, 353, 392, 500, 595, 679, 683, 946
NlaIV	GGNNCC	6 cut(s)	569, 570, 646, 663, 846, 1120
NmuCI	GTSAC	2 cut(s)	246, 868
NsiI	ATGCAT	3 cut(s)	681, 914, 948
PagI	TCATGA	2 cut(s)	279, 496
PciSI	GCTCTTC	2 cut(s)	984, 1024
PdmI	GAANNNNTTC	2 cut(s)	116, 1123
PfeI	GAWTC	1 cut(s)	1076
PflFI	GACNNNGTC	1 cut(s)	7
PkrI	GCNGC	2 cut(s)	142, 746
PleI	GAGTC	1 cut(s)	497
PpsI	GAGTC	1 cut(s)	497
PpuMI	RGGWCCY	1 cut(s)	568
Psp124BI	GAGCTC	1 cut(s)	1036
Psp5II	RGGWCCY	1 cut(s)	568
Psp6I	CCWGG	2 cut(s)	1003, 1012
PspFI	CCCAGC	2 cut(s)	641, 1061
PspGI	CCWGG	2 cut(s)	1003, 1012
PspN4I	GGNNCC	6 cut(s)	569, 570, 646, 663, 846, 1120
PspPI	GGNCC	4 cut(s)	191, 568, 645, 844
PspPPI	RGGWCCY	1 cut(s)	568
PsyI	GACNNNGTC	1 cut(s)	7
RsaI	GTAC	3 cut(s)	387, 815, 1143
RsaNI	GTAC	3 cut(s)	386, 814, 1142
RseI	CAYNNNNRTG	1 cut(s)	318
SacI	GAGCTC	1 cut(s)	1036
SacII	CCGCGG	1 cut(s)	867
SapI	GCTCTTC	2 cut(s)	984, 1024
SaqAI	TTAA	2 cut(s)	627, 770
SatI	GCNGC	2 cut(s)	141, 745
Sau3AI	GATC	6 cut(s)	613, 874, 930, 960, 1016, 1097
Sau96I	GGNCC	4 cut(s)	191, 568, 645, 844
SchI	GAGTC	1 cut(s)	497
ScrFI	CCNGG	3 cut(s)	1005, 1014, 1151
SduI	GDGCHC	2 cut(s)	349, 1036
SfaNI	GCATC	2 cut(s)	955, 1055
Sfr303I	CCGCGG	1 cut(s)	867
SgrBI	CCGCGG	1 cut(s)	867
SinI	GGWCC	3 cut(s)	568, 645, 844
SmiMI	CAYNNNNRTG	1 cut(s)	318
SmlI	CTYRAG	1 cut(s)	55
SmoI	CTYRAG	1 cut(s)	55
Sse9I	AATT	4 cut(s)	93, 328, 754, 1081
SsiI	CCGC	7 cut(s)	183, 316, 448, 744, 864, 866, 1110
SspMI	CTAG	1 cut(s)	999
SstI	GAGCTC	1 cut(s)	1036
StyD4I	CCNGG	3 cut(s)	1003, 1012, 1149
StyI	CCWWGG	1 cut(s)	147
TaiI	ACGT	2 cut(s)	627, 1162
TaqI	TCGA	2 cut(s)	795, 1100
TasI	AATT	4 cut(s)	93, 328, 754, 1081
TatI	WGTACW	2 cut(s)	813, 1141
TauI	GCSGC	1 cut(s)	747
TfiI	GAWTC	1 cut(s)	1076
Tru1I	TTAA	2 cut(s)	627, 770
Tru9I	TTAA	2 cut(s)	627, 770
TscAI	CASTG	3 cut(s)	240, 877, 1063
TseFI	GTSAC	2 cut(s)	246, 868
TseI	GCWGC	1 cut(s)	140
Tsp45I	GTSAC	2 cut(s)	246, 868
TspDTI	ATGAA	6 cut(s)	90, 268, 296, 390, 485, 513
TspRI	CASTG	3 cut(s)	240, 877, 1063
Tth111I	GACNNNGTC	1 cut(s)	7
VpaK11BI	GGWCC	3 cut(s)	568, 645, 844
XapI	RAATTY	3 cut(s)	328, 754, 1081
XcmI	CCANNNNNNNNNTGG	2 cut(s)	655, 949
XmnI	GAANNNNTTC	2 cut(s)	116, 1123
XspI	CTAG	1 cut(s)	999
Zsp2I	ATGCAT	3 cut(s)	681, 914, 948

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.