Rw7G003220

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015922
Physical Location & Seq
Reverse (-)
2614880 .. 2615593
714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G003220.1

Sequence Viewer

Length: 714 bp
ATGGATTCCATCAACGTCCTCAAAGGCTACGGCAAGGTAAACCCAGAACACCACCTAGAAGTCCAAGCTCAGAAGATCCCCCAACCACCCAAAAGGCCAATCATCACCACTCTTGTCATCTCAGCAATAGTCGGCTTGACCTTTGTGATCGGCCTCATGCTCGCAGCTCTCATCCATGAATCCGCTACCGAGCCAATCGACGAGTCCCCATCCCTCTCCAGCTCTGCCGAGTCAACCATCAAAATCGTCTGCAACGTGACTCGGTACCCCGAGTCTTGCTTCACCTCCATTTCCTCCCTCAACAGCTCTCCCAAACCAGACCCTGAGTCCATCCTCAAGCTCTCTCTCCAAGTCTCCATTTCCGAGCTTTCCCATCTCTCTTCGGTGTTGAAGACCATGAACTCCGATGCCGCCATCCGTGACTGCCGGGACCAGGTGGAGGACGCGTTGAGTCAGCTCGATGACTCGGTGTCGGCAATGGTGGTGGGCAAAGCGTTGACTGAAGCAAAGGTCAAGGACGTCCAGACGTGGATAAGTAGTGCGGTGACGGACCAAGAGACGTGCTTGGATGGTTTGGAAGAGATGGGATCAGCTAGTGTTGATGAAGTGAAGAAGAAGATAAAGAAGTCGATGGACTTCACCAGCAACAGCTTAGCCATTGTGGCTAATTTCAAGGCCATTCTTGACAGGTTTAGCCACATTCCACTCCATTAG

Protein Analysis

237

Amino Acids

25.63

Weight (kDa)

5.25

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 79 - 220 8.2e-33 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015922)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47670
fragaria_vesca FvH4_5g15211
malus_domestica MD06G1191000.v1.1
prunus_persica Prupe.5G189700_v2.0.a1
pyrus_communis pycom06g16930
rosa_chinensis RchiOBHm_Chr7g0180951
rosa_laevigata RLG00000005222
rosa_multiflora Rmu_co8153612.1_g000001 Rmu_sc0028417.1_g000005
rosa_roxburghii Rroxscaffold_3G00272390
rosa_rugosa Rorug06G0437900
rosa_samantha Rh7AG039300 Rh7BG038600 Rh7CG040300
rosa_wichuraiana Rw7G003220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 522
Acc65I GGTACC 1 cut(s) 264
AccB1I GGYRCC 1 cut(s) 264
AccII CGCG 1 cut(s) 446
AciI CCGC 3 cut(s) 183, 411, 542
AclWI GGATC 2 cut(s) 70, 595
AcuI CTGAAG 1 cut(s) 522
AcyI GRCGYC 1 cut(s) 519
AfaI GTAC 1 cut(s) 266
AfiI CCNNNNNNNGG 2 cut(s) 433, 439
AflIII ACRYGT 1 cut(s) 444
AgsI TTSAA 2 cut(s) 391, 673
AhdI GACNNNNNGTC 2 cut(s) 325, 469
AjiI CACGTC 2 cut(s) 528, 561
AjnI CCWGG 1 cut(s) 432
AluBI AGCT 9 cut(s) 68, 167, 222, 306, 340, 367, 457, 593, 651
AluI AGCT 9 cut(s) 68, 167, 222, 306, 340, 367, 457, 593, 651
Alw26I GTCTC 2 cut(s) 358, 551
AlwI GGATC 2 cut(s) 70, 595
AlwNI CAGNNNCTG 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 269
AoxI GGCC 3 cut(s) 95, 151, 675
ApeKI GCWGC 1 cut(s) 164
Asp718I GGTACC 1 cut(s) 264
AspS9I GGNCC 2 cut(s) 430, 550
AsuC2I CCSGG 1 cut(s) 428
AsuHPI GGTGA 4 cut(s) 97, 274, 556, 631
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 2 cut(s) 430, 550
BanI GGYRCC 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 398
BbvI GCAGC 1 cut(s) 176
BccI CCATC 9 cut(s) 17, 217, 245, 338, 381, 422, 563, 577, 625
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 434
BcnI CCSGG 1 cut(s) 428
BcoDI GTCTC 2 cut(s) 358, 551
BfaI CTAG 2 cut(s) 56, 594
BglI GCCNNNNNGGC 1 cut(s) 662
BisI GCNGC 2 cut(s) 165, 411
BlpI GCTNAGC 1 cut(s) 652
BlsI GCNGC 2 cut(s) 166, 412
Bme1390I CCNGG 2 cut(s) 428, 434
Bme18I GGWCC 2 cut(s) 430, 550
BmeRI GACNNNNNGTC 2 cut(s) 325, 469
BmeT110I CYCGRG 1 cut(s) 269
BmgBI CACGTC 2 cut(s) 528, 561
BmgT120I GGNCC 2 cut(s) 430, 550
BmiI GGNNCC 2 cut(s) 266, 431
BmrFI CCNGG 2 cut(s) 428, 434
BmsI GCATC 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 398
BpmI CTGGAG 1 cut(s) 202
Bpu1102I GCTNAGC 1 cut(s) 652
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 428
BsaHI GRCGYC 1 cut(s) 519
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 2 cut(s) 433, 439
Bse3DI GCAATG 1 cut(s) 483
BseBI CCWGG 1 cut(s) 434
BseGI GGATG 5 cut(s) 171, 209, 330, 414, 574
BseLI CCNNNNNNNGG 2 cut(s) 433, 439
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 3 cut(s) 83, 135, 315
BseXI GCAGC 1 cut(s) 176
Bsh1236I CGCG 1 cut(s) 446
BshFI GGCC 3 cut(s) 97, 153, 677
BshNI GGYRCC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 1 cut(s) 427
BslFI GGGAC 2 cut(s) 190, 443
BslI CCNNNNNNNGG 2 cut(s) 433, 439
BsmAI GTCTC 2 cut(s) 358, 551
BsmBI CGTCTC 1 cut(s) 551
BsmFI GGGAC 2 cut(s) 190, 443
BsnI GGCC 3 cut(s) 97, 153, 677
BsoBI CYCGRG 1 cut(s) 269
Bsp143I GATC 3 cut(s) 75, 147, 587
Bsp1720I GCTNAGC 1 cut(s) 652
BspACI CCGC 3 cut(s) 183, 411, 542
BspANI GGCC 3 cut(s) 97, 153, 677
BspCNI CTCAG 3 cut(s) 82, 134, 316
BspFNI CGCG 1 cut(s) 446
BspLI GGNNCC 2 cut(s) 266, 431
BspPI GGATC 2 cut(s) 70, 595
BspT107I GGYRCC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 483
BssMI GATC 3 cut(s) 75, 147, 587
BssNI GRCGYC 1 cut(s) 519
Bst2UI CCWGG 1 cut(s) 434
Bst6I CTCTTC 2 cut(s) 385, 573
BstACI GRCGYC 1 cut(s) 519
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 4 cut(s) 69, 121, 324, 652
BstF5I GGATG 5 cut(s) 171, 209, 330, 414, 574
BstFNI CGCG 1 cut(s) 446
BstKTI GATC 3 cut(s) 78, 150, 590
BstMAI GTCTC 2 cut(s) 358, 551
BstMBI GATC 3 cut(s) 75, 147, 587
BstMWI GCNNNNNNNGC 1 cut(s) 662
BstNI CCWGG 1 cut(s) 434
BstSCI CCNGG 2 cut(s) 426, 432
BstUI CGCG 1 cut(s) 446
BstV1I GCAGC 1 cut(s) 176
BstV2I GAAGAC 1 cut(s) 398
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BsuRI GGCC 3 cut(s) 97, 153, 677
BtrI CACGTC 2 cut(s) 528, 561
BtsCI GGATG 5 cut(s) 171, 209, 330, 414, 574
Cac8I GCNNGC 1 cut(s) 162
CaiI CAGNNNCTG 1 cut(s) 323
Cfr13I GGNCC 2 cut(s) 430, 550
CseI GACGC 1 cut(s) 452
CsiI ACCWGGT 1 cut(s) 432
Csp6I GTAC 1 cut(s) 265
CspCI CAANNNNNGTGG 2 cut(s) 465, 500
CviAII CATG 3 cut(s) 157, 176, 397
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 4 cut(s) 69, 121, 324, 652
DpnI GATC 3 cut(s) 77, 149, 589
DpnII GATC 3 cut(s) 75, 147, 587
DriI GACNNNNNGTC 2 cut(s) 325, 469
Eam1104I CTCTTC 2 cut(s) 385, 573
Eam1105I GACNNNNNGTC 2 cut(s) 325, 469
EarI CTCTTC 2 cut(s) 385, 573
Eco47I GGWCC 2 cut(s) 430, 550
Eco57I CTGAAG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 432
Esp3I CGTCTC 1 cut(s) 551
FaeI CATG 3 cut(s) 160, 179, 400
FaiI YATR 3 cut(s) 158, 177, 398
FaqI GGGAC 2 cut(s) 190, 443
FatI CATG 3 cut(s) 156, 175, 396
Fnu4HI GCNGC 2 cut(s) 165, 411
FokI GGATG 5 cut(s) 158, 196, 317, 401, 581
Fsp4HI GCNGC 2 cut(s) 165, 411
FspBI CTAG 2 cut(s) 56, 594
GluI GCNGC 2 cut(s) 165, 411
GsuI CTGGAG 1 cut(s) 202
HaeIII GGCC 3 cut(s) 97, 153, 677
HapII CCGG 1 cut(s) 427
HgaI GACGC 1 cut(s) 452
Hin1I GRCGYC 1 cut(s) 519
Hin1II CATG 3 cut(s) 160, 179, 400
HincII GTYRAC 2 cut(s) 234, 498
HindII GTYRAC 2 cut(s) 234, 498
HinfI GANTC 9 cut(s) 5, 179, 203, 230, 259, 272, 326, 451, 464
HpaII CCGG 1 cut(s) 427
HphI GGTGA 4 cut(s) 97, 274, 556, 631
Hpy166II GTNNAC 3 cut(s) 40, 234, 498
Hpy188I TCNGA 3 cut(s) 72, 364, 406
Hpy188III TCNNGA 2 cut(s) 523, 683
Hpy8I GTNNAC 3 cut(s) 40, 234, 498
Hpy99I CGWCG 1 cut(s) 203
HpyCH4IV ACGT 5 cut(s) 15, 255, 519, 527, 560
HpyCH4V TGCA 1 cut(s) 252
HpyF10VI GCNNNNNNNGC 1 cut(s) 662
HpyF3I CTNAG 4 cut(s) 69, 121, 324, 652
HpySE526I ACGT 5 cut(s) 15, 255, 519, 527, 560
Hsp92I GRCGYC 1 cut(s) 519
Hsp92II CATG 3 cut(s) 160, 179, 400
KpnI GGTACC 1 cut(s) 268
Kzo9I GATC 3 cut(s) 75, 147, 587
Lsp1109I GCAGC 1 cut(s) 176
LweI GCATC 1 cut(s) 397
MabI ACCWGGT 1 cut(s) 432
MaeI CTAG 2 cut(s) 56, 594
MaeII ACGT 5 cut(s) 15, 255, 519, 527, 560
MaeIII GTNAC 3 cut(s) 256, 419, 544
MalI GATC 3 cut(s) 77, 149, 589
MboI GATC 3 cut(s) 75, 147, 587
MboII GAAGA 7 cut(s) 85, 372, 403, 590, 622, 625, 628
MflI RGATCY 1 cut(s) 75
MluCI AATT 1 cut(s) 667
MluI ACGCGT 1 cut(s) 444
MlyI GAGTC 7 cut(s) 212, 239, 253, 281, 335, 458, 460
MnlI CCTC 8 cut(s) 29, 164, 224, 295, 304, 308, 344, 433
MspI CCGG 1 cut(s) 427
MspR9I CCNGG 2 cut(s) 428, 434
MvaI CCWGG 1 cut(s) 434
MvnI CGCG 1 cut(s) 446
MwoI GCNNNNNNNGC 1 cut(s) 662
NciI CCSGG 1 cut(s) 428
NdeII GATC 3 cut(s) 75, 147, 587
NlaIII CATG 3 cut(s) 160, 179, 400
NlaIV GGNNCC 2 cut(s) 266, 431
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 3 cut(s) 256, 419, 544
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 2 cut(s) 5, 179
PkrI GCNGC 2 cut(s) 166, 412
PleI GAGTC 7 cut(s) 211, 238, 253, 280, 334, 458, 459
PpsI GAGTC 7 cut(s) 211, 238, 253, 280, 334, 458, 459
Psp6I CCWGG 1 cut(s) 432
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 2 cut(s) 266, 431
PspPI GGNCC 2 cut(s) 430, 550
PstNI CAGNNNCTG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 75
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
SatI GCNGC 2 cut(s) 165, 411
Sau3AI GATC 3 cut(s) 75, 147, 587
Sau96I GGNCC 2 cut(s) 430, 550
SchI GAGTC 7 cut(s) 212, 239, 253, 281, 335, 458, 460
ScrFI CCNGG 2 cut(s) 428, 434
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 1 cut(s) 397
SinI GGWCC 2 cut(s) 430, 550
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 1 cut(s) 667
SsiI CCGC 3 cut(s) 183, 411, 542
SspMI CTAG 2 cut(s) 56, 594
StyD4I CCNGG 2 cut(s) 426, 432
TaiI ACGT 5 cut(s) 18, 258, 522, 530, 563
TaqI TCGA 3 cut(s) 198, 459, 629
TasI AATT 1 cut(s) 667
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 2 cut(s) 5, 179
TseFI GTSAC 3 cut(s) 256, 419, 544
TseI GCWGC 1 cut(s) 164
Tsp45I GTSAC 3 cut(s) 256, 419, 544
TspDTI ATGAA 3 cut(s) 192, 413, 618
TspGWI ACGGA 2 cut(s) 407, 563
VpaK11BI GGWCC 2 cut(s) 430, 550
XspI CTAG 2 cut(s) 56, 594
ZraI GACGTC 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.