Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0005779

Physical Location & Seq

Genomic Coordinates Reverse (-)

11965462 .. 11969762

Gene Structure 4301 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G012610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGCCTGGACACGAAGTTGCCAAAGATGACATGATGATCGATGAGTATGAACAGAAGCAAGTGAATGCTGTTCCTGATGGATTTAACTCTCAGTATCTCAAAATTTACTATGGAAAGCTCTTCCCATATGAGGAAATGTTTAAATGGATGTCCTATGCAAATGATGGCAAGCATCCTGCATGCAACCAGTCCTACTTTGGCCGGAGAGAGTTTTCTTTCACACTGGATAATGATGTTTATCTGCGGTACAAAACTTTCAACTCCGTGTCTGAGCTTGAAAATTCTATCAAAGAGAAGTGCCCTGTCAAAATAGATATTGGGCCTGTGTACAATGTTGATCCTGCAAAGAGGCATTCCTATGCTCAAAGTGGTTGCTACCCCGAGGAGAGGGAACTGATTTTTGATATAGATATGTCAGATTACGATGATGTTAGATACTGCTGCTCAGGAGCTGATGTCTGCCTGGAGTGCTGGCCGCTTATGACAATTGCTATCAAAGTGATTGACACTGCCCTTCGAGATGACTTTGGTTTCAATCACATTCTCTGGGTATATAGTGGCCGACGTGGTGTTCATTGTTGGGTTTGTGATGGGAAAGCTAGAAGGCTCACAAATGAACTGAGAGCAGCTATTGCTGACTATTTTCGTGTCTACAAGGGCAATGAAAACAGTCATAAAAAGGTTTCGTTGATGGGCCCTGCTCTTCATCCATTCCTTGTGAGATCATATACTGAAGTTCTTAAGGATTTCTTTGAGAGGACATTGCTTTCCAGTCAAAAGATATTTTCTACTGCAGAGAGATATGAAAAGATCTTAGATATGATTCCTGATGAATCTGTCACTTCTGAGCTTCGGGGAAAGTGGCAAGATAATAGACGAACCTCCAATGCAAAAGAAGACATTAATGTTGCTCGTTGGGAGCAGCTGAAACATTTGCTGCAATCTGGGAAACAGAAGATGCAAGGACTGCGTAGATGTGTGGAGGAGATTGTGTTCTCTTATACCTACCCTAGACTTGACATGGAGGTTTCAAAACATATGAACCATTTATTAAAGGCACCATTCTGTGTACATCCAAAGACAGGTCGTGTTTGTGTTCCAATTGATCCCAACCATTGTGAGGATTTTGATCCTACTGCAGTTCCAACCCTTTCCACTCTTATAGAAGAACTTAATAATGAGGGTTTGAGAGCAGAGGCTGATAACGACCGGGATAGAACGTCACTTGGAAAATCAATAAGATTCTTCCAATCATCATTTTTGGAGCCTTTGGTAAAATCTTGCAAGGAGGAGATGATAAGTTCCTACAATGCGAAACTGCAGCAGTCGAAGTTGCAGCAGTCAAAGAACGTGCTTGCTGCAATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0003896 GO:0003899 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0032993 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_primase_S

Protein Analysis

455

Amino Acids

52.77

Weight (kDa)

6.22

Isoelectric Point (pI)

51.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_primase_S	PF01896	134 - 369	3.6e-54	DNA primase small subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005779)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G41880
fragaria_vesca	FvH4_5g04460
malus_domestica	MD06G1105400.v1.1
prunus_persica	Prupe.5G117700_v2.0.a1
pyrus_communis	pycom06g09970
rosa_chinensis	RchiOBHm_Chr7g0195041
rosa_laevigata	RLG00000004150 RLG00000008501 RLG00000015305
rosa_multiflora	Rmu_sc0002246.1_g000003
rosa_roxburghii	Rroxscaffold_3G00260810
rosa_rugosa	Rorug07G0020100 Rorug07G0020200
rosa_samantha	Rh6BG025100 Rh6BG025600 Rh6BG025700 Rh6BG026500 Rh6BG026600 Rh6CG024600 Rh6DG024100 Rh6DG024400 Rh7AG147700 Rh7BG148300 Rh7CG152000
rosa_wichuraiana	Rw2G021680 Rw5G042100 Rw6G002490 Rw7G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1057
AccI	GTMKAC	1 cut(s)	651
AciI	CCGC	2 cut(s)	244, 476
AclWI	GGATC	3 cut(s)	332, 1100, 1124
AcoI	YGGCCR	3 cut(s)	199, 473, 559
AcsI	RAATTY	2 cut(s)	102, 280
AcuI	CTGAAG	1 cut(s)	753
AfaI	GTAC	3 cut(s)	248, 329, 1071
AfiI	CCNNNNNNNGG	4 cut(s)	130, 387, 1082, 1120
AflII	CTTAAG	1 cut(s)	740
AgsI	TTSAA	4 cut(s)	259, 278, 535, 1032
AjiI	CACGTC	1 cut(s)	566
AjnI	CCWGG	2 cut(s)	4, 462
AloI	GAACNNNNNNTCC	2 cut(s)	977, 1009
AluBI	AGCT	7 cut(s)	118, 274, 452, 599, 629, 850, 925
AluI	AGCT	7 cut(s)	118, 274, 452, 599, 629, 850, 925
AlwI	GGATC	3 cut(s)	332, 1100, 1124
AlwNI	CAGNNNCTG	2 cut(s)	452, 1199
Ama87I	CYCGRG	1 cut(s)	380
AoxI	GGCC	5 cut(s)	199, 320, 473, 559, 694
ApaI	GGGCCC	1 cut(s)	698
ApeKI	GCWGC	7 cut(s)	441, 626, 922, 937, 1321, 1336, 1358
ApoI	RAATTY	2 cut(s)	102, 280
AseI	ATTAAT	1 cut(s)	903
AspS9I	GGNCC	3 cut(s)	320, 694, 695
AsuC2I	CCSGG	1 cut(s)	1211
AvaI	CYCGRG	1 cut(s)	380
BaeGI	GKGCMC	2 cut(s)	302, 698
BanI	GGYRCC	1 cut(s)	1057
BanII	GRGCYC	1 cut(s)	698
BbsI	GAAGAC	1 cut(s)	903
BbvI	GCAGC	7 cut(s)	428, 638, 924, 934, 1333, 1345, 1348
BccI	CCATC	4 cut(s)	71, 158, 584, 685
BciT130I	CCWGG	2 cut(s)	6, 464
BcnI	CCSGG	1 cut(s)	1211
BfaI	CTAG	3 cut(s)	600, 1011, 1366
BfmI	CTRYAG	3 cut(s)	792, 1137, 1319
BfrI	CTTAAG	1 cut(s)	740
BglII	AGATCT	1 cut(s)	810
BisI	GCNGC	8 cut(s)	442, 476, 627, 923, 938, 1322, 1337, 1359
BlsI	GCNGC	8 cut(s)	443, 477, 628, 924, 939, 1323, 1338, 1360
Bme1390I	CCNGG	3 cut(s)	6, 464, 1211
BmeT110I	CYCGRG	1 cut(s)	380
BmgBI	CACGTC	1 cut(s)	566
BmgT120I	GGNCC	3 cut(s)	320, 694, 695
BmiI	GGNNCC	3 cut(s)	696, 1059, 1266
BmrFI	CCNGG	3 cut(s)	6, 464, 1211
BmsI	GCATC	2 cut(s)	181, 948
BpiI	GAAGAC	1 cut(s)	903
BpmI	CTGGAG	1 cut(s)	485
Bpu10I	CCTNAGC	1 cut(s)	445
BpuMI	CCSGG	1 cut(s)	1211
Bsa29I	ATCGAT	1 cut(s)	39
BsaBI	GATNNNNATC	2 cut(s)	237, 1128
BsaJI	CCNNGG	1 cut(s)	381
BsaXI	ACNNNNNCTCC	4 cut(s)	441, 471, 977, 1007
Bsc4I	CCNNNNNNNGG	4 cut(s)	130, 387, 1082, 1120
Bse1I	ACTGG	3 cut(s)	187, 228, 771
Bse3DI	GCAATG	2 cut(s)	667, 761
Bse8I	GATNNNNATC	2 cut(s)	237, 1128
BseBI	CCWGG	2 cut(s)	6, 464
BseCI	ATCGAT	1 cut(s)	39
BseDI	CCNNGG	1 cut(s)	381
BseGI	GGATG	4 cut(s)	153, 172, 706, 1072
BseJI	GATNNNNATC	2 cut(s)	237, 1128
BseLI	CCNNNNNNNGG	4 cut(s)	130, 387, 1082, 1120
BseMI	GCAATG	2 cut(s)	667, 761
BseMII	CTCAG	5 cut(s)	104, 261, 459, 611, 837
BseNI	ACTGG	3 cut(s)	187, 228, 771
BseRI	GAGGAG	3 cut(s)	398, 998, 1304
BseSI	GKGCMC	2 cut(s)	302, 698
BseXI	GCAGC	7 cut(s)	428, 638, 924, 934, 1333, 1345, 1348
Bsh1285I	CGRYCG	1 cut(s)	1210
BshFI	GGCC	5 cut(s)	201, 322, 475, 561, 696
BshNI	GGYRCC	1 cut(s)	1057
BshVI	ATCGAT	1 cut(s)	39
BsiEI	CGRYCG	1 cut(s)	1210
BsiHKCI	CYCGRG	1 cut(s)	380
BsiSI	CCGG	2 cut(s)	202, 1210
BslI	CCNNNNNNNGG	4 cut(s)	130, 387, 1082, 1120
BsmI	GAATGC	2 cut(s)	70, 352
BsnI	GGCC	5 cut(s)	201, 322, 475, 561, 696
BsoBI	CYCGRG	1 cut(s)	380
Bsp120I	GGGCCC	1 cut(s)	694
Bsp1286I	GDGCHC	2 cut(s)	302, 698
Bsp1407I	TGTACA	2 cut(s)	327, 1069
Bsp143I	GATC	6 cut(s)	36, 337, 722, 810, 1105, 1129
BspACI	CCGC	2 cut(s)	244, 476
BspANI	GGCC	5 cut(s)	201, 322, 475, 561, 696
BspCNI	CTCAG	5 cut(s)	103, 262, 458, 612, 838
BspDI	ATCGAT	1 cut(s)	39
BspLI	GGNNCC	3 cut(s)	696, 1059, 1266
BspMAI	CTGCAG	3 cut(s)	796, 1141, 1323
BspPI	GGATC	3 cut(s)	332, 1100, 1124
BspQI	GCTCTTC	2 cut(s)	125, 708
BspT107I	GGYRCC	1 cut(s)	1057
BspTI	CTTAAG	1 cut(s)	740
BsrDI	GCAATG	2 cut(s)	667, 761
BsrGI	TGTACA	2 cut(s)	327, 1069
BsrI	ACTGG	3 cut(s)	187, 228, 771
BssECI	CCNNGG	1 cut(s)	381
BssMI	GATC	6 cut(s)	36, 337, 722, 810, 1105, 1129
Bst2UI	CCWGG	2 cut(s)	6, 464
Bst4CI	ACNGT	1 cut(s)	671
Bst6I	CTCTTC	2 cut(s)	125, 708
BstAFI	CTTAAG	1 cut(s)	740
BstAPI	GCANNNNNTGC	2 cut(s)	632, 967
BstAUI	TGTACA	2 cut(s)	327, 1069
BstC8I	GCNNGC	4 cut(s)	170, 181, 473, 1356
BstDEI	CTNAG	6 cut(s)	90, 270, 445, 620, 814, 846
BstF5I	GGATG	4 cut(s)	153, 172, 706, 1072
BstKTI	GATC	6 cut(s)	39, 340, 725, 813, 1108, 1132
BstMBI	GATC	6 cut(s)	36, 337, 722, 810, 1105, 1129
BstMCI	CGRYCG	1 cut(s)	1210
BstMWI	GCNNNNNNNGC	3 cut(s)	468, 632, 967
BstNI	CCWGG	2 cut(s)	6, 464
BstNSI	RCATGY	1 cut(s)	183
BstSCI	CCNGG	3 cut(s)	4, 462, 1209
BstSFI	CTRYAG	3 cut(s)	792, 1137, 1319
BstSLI	GKGCMC	2 cut(s)	302, 698
BstV1I	GCAGC	7 cut(s)	428, 638, 924, 934, 1333, 1345, 1348
BstV2I	GAAGAC	1 cut(s)	903
BstX2I	RGATCY	1 cut(s)	810
BstYI	RGATCY	1 cut(s)	810
Bsu15I	ATCGAT	1 cut(s)	39
BsuRI	GGCC	5 cut(s)	201, 322, 475, 561, 696
BsuTUI	ATCGAT	1 cut(s)	39
BtrI	CACGTC	1 cut(s)	566
BtsCI	GGATG	4 cut(s)	153, 172, 706, 1072
BtsI	GCAGTG	1 cut(s)	507
BtsIMutI	CAGTG	2 cut(s)	221, 507
Cac8I	GCNNGC	4 cut(s)	170, 181, 473, 1356
CaiI	CAGNNNCTG	2 cut(s)	452, 1199
Cfr13I	GGNCC	3 cut(s)	320, 694, 695
ClaI	ATCGAT	1 cut(s)	39
Csp6I	GTAC	3 cut(s)	247, 328, 1070
CviAII	CATG	3 cut(s)	31, 180, 1021
CviQI	GTAC	3 cut(s)	247, 328, 1070
DdeI	CTNAG	6 cut(s)	90, 270, 445, 620, 814, 846
DpnI	GATC	6 cut(s)	38, 339, 724, 812, 1107, 1131
DpnII	GATC	6 cut(s)	36, 337, 722, 810, 1105, 1129
DraI	TTTAAA	1 cut(s)	142
EaeI	YGGCCR	3 cut(s)	199, 473, 559
Eam1104I	CTCTTC	2 cut(s)	125, 708
EarI	CTCTTC	2 cut(s)	125, 708
Eco24I	GRGCYC	1 cut(s)	698
Eco57I	CTGAAG	1 cut(s)	753
Eco88I	CYCGRG	1 cut(s)	380
EcoO109I	RGGNCCY	1 cut(s)	695
EcoRII	CCWGG	2 cut(s)	4, 462
EcoT38I	GRGCYC	1 cut(s)	698
FaeI	CATG	3 cut(s)	34, 183, 1024
FalI	AAGNNNNNCTT	2 cut(s)	734, 766
FatI	CATG	3 cut(s)	30, 179, 1020
FauNDI	CATATG	2 cut(s)	127, 1038
FblI	GTMKAC	1 cut(s)	651
Fnu4HI	GCNGC	8 cut(s)	442, 476, 627, 923, 938, 1322, 1337, 1359
FokI	GGATG	4 cut(s)	159, 160, 693, 1059
FriOI	GRGCYC	1 cut(s)	698
Fsp4HI	GCNGC	8 cut(s)	442, 476, 627, 923, 938, 1322, 1337, 1359
FspBI	CTAG	3 cut(s)	600, 1011, 1366
GluI	GCNGC	8 cut(s)	442, 476, 627, 923, 938, 1322, 1337, 1359
GsuI	CTGGAG	1 cut(s)	485
HaeIII	GGCC	5 cut(s)	201, 322, 475, 561, 696
HapII	CCGG	2 cut(s)	202, 1210
Hin1II	CATG	3 cut(s)	34, 183, 1024
HinfI	GANTC	3 cut(s)	823, 833, 1242
HpaII	CCGG	2 cut(s)	202, 1210
Hpy166II	GTNNAC	3 cut(s)	328, 652, 1070
Hpy188I	TCNGA	3 cut(s)	271, 418, 847
Hpy188III	TCNNGA	4 cut(s)	74, 447, 518, 827
Hpy8I	GTNNAC	3 cut(s)	328, 652, 1070
Hpy99I	CGWCG	1 cut(s)	567
HpyAV	CCTTC	2 cut(s)	524, 597
HpyCH4III	ACNGT	1 cut(s)	671
HpyCH4IV	ACGT	3 cut(s)	565, 1220, 1350
HpyF10VI	GCNNNNNNNGC	3 cut(s)	468, 632, 967
HpyF3I	CTNAG	6 cut(s)	90, 270, 445, 620, 814, 846
HpySE526I	ACGT	3 cut(s)	565, 1220, 1350
Hsp92II	CATG	3 cut(s)	34, 183, 1024
Kzo9I	GATC	6 cut(s)	36, 337, 722, 810, 1105, 1129
LguI	GCTCTTC	2 cut(s)	125, 708
LmnI	GCTCC	3 cut(s)	449, 919, 1264
Lsp1109I	GCAGC	7 cut(s)	428, 638, 924, 934, 1333, 1345, 1348
LweI	GCATC	2 cut(s)	181, 948
MaeI	CTAG	3 cut(s)	600, 1011, 1366
MaeII	ACGT	3 cut(s)	565, 1220, 1350
MaeIII	GTNAC	2 cut(s)	838, 1221
MalI	GATC	6 cut(s)	38, 339, 724, 812, 1107, 1131
MboI	GATC	6 cut(s)	36, 337, 722, 810, 1105, 1129
MboII	GAAGA	6 cut(s)	112, 695, 908, 967, 1178, 1237
MfeI	CAATTG	2 cut(s)	486, 1101
MflI	RGATCY	1 cut(s)	810
MhlI	GDGCHC	2 cut(s)	302, 698
MluCI	AATT	4 cut(s)	102, 280, 486, 1101
MmeI	TCCRAC	1 cut(s)	1169
MseI	TTAA	6 cut(s)	84, 141, 741, 903, 1052, 1173
MslI	CAYNNNNRTG	1 cut(s)	357
MspA1I	CMGCKG	1 cut(s)	925
MspCI	CTTAAG	1 cut(s)	740
MspI	CCGG	2 cut(s)	202, 1210
MspR9I	CCNGG	3 cut(s)	6, 464, 1211
MunI	CAATTG	2 cut(s)	486, 1101
Mva1269I	GAATGC	2 cut(s)	70, 352
MvaI	CCWGG	2 cut(s)	6, 464
MwoI	GCNNNNNNNGC	3 cut(s)	468, 632, 967
NciI	CCSGG	1 cut(s)	1211
NdeI	CATATG	2 cut(s)	127, 1038
NdeII	GATC	6 cut(s)	36, 337, 722, 810, 1105, 1129
NlaIII	CATG	3 cut(s)	34, 183, 1024
NlaIV	GGNNCC	3 cut(s)	696, 1059, 1266
NmuCI	GTSAC	2 cut(s)	838, 1221
NspI	RCATGY	1 cut(s)	183
PaeI	GCATGC	1 cut(s)	183
PciSI	GCTCTTC	2 cut(s)	125, 708
PctI	GAATGC	2 cut(s)	70, 352
PfeI	GAWTC	3 cut(s)	823, 833, 1242
PkrI	GCNGC	8 cut(s)	443, 477, 628, 924, 939, 1323, 1338, 1360
PshBI	ATTAAT	1 cut(s)	903
Psp6I	CCWGG	2 cut(s)	4, 462
PspGI	CCWGG	2 cut(s)	4, 462
PspN4I	GGNNCC	3 cut(s)	696, 1059, 1266
PspOMI	GGGCCC	1 cut(s)	694
PspPI	GGNCC	3 cut(s)	320, 694, 695
PstI	CTGCAG	3 cut(s)	796, 1141, 1323
PstNI	CAGNNNCTG	2 cut(s)	452, 1199
PsuI	RGATCY	1 cut(s)	810
PvuII	CAGCTG	1 cut(s)	925
RsaI	GTAC	3 cut(s)	248, 329, 1071
RsaNI	GTAC	3 cut(s)	247, 328, 1070
RseI	CAYNNNNRTG	1 cut(s)	357
SapI	GCTCTTC	2 cut(s)	125, 708
SaqAI	TTAA	6 cut(s)	84, 141, 741, 903, 1052, 1173
SatI	GCNGC	8 cut(s)	442, 476, 627, 923, 938, 1322, 1337, 1359
Sau3AI	GATC	6 cut(s)	36, 337, 722, 810, 1105, 1129
Sau96I	GGNCC	3 cut(s)	320, 694, 695
ScrFI	CCNGG	3 cut(s)	6, 464, 1211
SduI	GDGCHC	2 cut(s)	302, 698
SfaNI	GCATC	2 cut(s)	181, 948
SfcI	CTRYAG	3 cut(s)	792, 1137, 1319
SmiMI	CAYNNNNRTG	1 cut(s)	357
SmlI	CTYRAG	1 cut(s)	740
SmoI	CTYRAG	1 cut(s)	740
SphI	GCATGC	1 cut(s)	183
Sse9I	AATT	4 cut(s)	102, 280, 486, 1101
SsiI	CCGC	2 cut(s)	244, 476
SspMI	CTAG	3 cut(s)	600, 1011, 1366
StyD4I	CCNGG	3 cut(s)	4, 462, 1209
TaaI	ACNGT	1 cut(s)	671
TaiI	ACGT	3 cut(s)	568, 1223, 1353
TaqI	TCGA	3 cut(s)	39, 517, 1328
TasI	AATT	4 cut(s)	102, 280, 486, 1101
TatI	WGTACW	2 cut(s)	327, 1069
TauI	GCSGC	1 cut(s)	478
TfiI	GAWTC	3 cut(s)	823, 833, 1242
Tru1I	TTAA	6 cut(s)	84, 141, 741, 903, 1052, 1173
Tru9I	TTAA	6 cut(s)	84, 141, 741, 903, 1052, 1173
TscAI	CASTG	2 cut(s)	228, 514
TseFI	GTSAC	2 cut(s)	838, 1221
TseI	GCWGC	7 cut(s)	441, 626, 922, 937, 1321, 1336, 1358
Tsp45I	GTSAC	2 cut(s)	838, 1221
TspDTI	ATGAA	8 cut(s)	63, 563, 630, 678, 695, 819, 846, 1055
TspGWI	ACGGA	1 cut(s)	253
TspRI	CASTG	2 cut(s)	228, 514
Vha464I	CTTAAG	1 cut(s)	740
VspI	ATTAAT	1 cut(s)	903
XapI	RAATTY	2 cut(s)	102, 280
XceI	RCATGY	1 cut(s)	183
XcmI	CCANNNNNNNNNTGG	1 cut(s)	194
XmiI	GTMKAC	1 cut(s)	651
XspI	CTAG	3 cut(s)	600, 1011, 1366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.