Rw7G025470

Multiple chloroplast division site 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012484
Physical Location & Seq
Forward (+)
31806642 .. 31808620
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025470.1

Sequence Viewer

Length: 1038 bp
ATGGCTTCCACTTCGACTCTCCAATTCCACTCCCTTTCCTTCCAGACCTCAATTTGGGTCTCAAAGCACCAAACACCTGCATTGGTACTAAAGGTTAGAGCCCAATTCGGGTCCAGTAACGGTCGGTTCATCTGGGCACGACGGCTAAAACCCACTCACAGGTTCCTACTTAGAGCCATTGACGGCTCAGGTAGCTCTAATGGTGAAAACCCTAATGCCCATAATGATGAGAACTACAAGTATACCATAAACCTCAAAGACCCAGTTGACAAGTTCCACGGAATGATCAGCTCAGTGTCTCCCATTGTTTTCAAGATACGACAACGAGTAGGATCGAATTTGGCAATTGGTTTATGTATTGCAACTGCATTTTTGGTGGTTGCTCTGAGAGTATTCATGGTCAGGAAGTCGAAGAACAACCGTCAAGGCTCTGTAGCTGATCTTGTCAGACGTGGTCAGCTTAGGTCCGATAGAAGAGGCATTTCGAAGCCTCTCATGTACGATGACCCATTCAATAATCCATTGGTTAAAGTTGGTAAGGGAAATTCGACTATAGAGATGTGTGGAAAGGTTTATCGCCTTGCCCCAATTACTCTTACGAAAGAGGAACAAGCTATCCATCGAAAGAGGAGGTCGCGGGCATACCAGTGGAAGAGACCGACAATGTTCCTTAAAGAAGGGGATTCGATACCTGCTGATGTTGACCCGGATACAATTAGGTGGATTCCTTCCAATCATCCTTTTGCAACCACTGCCGGTGACATTGATGAAGACTTGGCACAGACCAATGTGTATCAGAAACATGGTGTACCGTATCGAATTCAGGCTGAGCATGAAGCACTGCAGAGAGAGCAAAAGGAGAGCAGGTTAGTGATGGACTCTAGCAATGCTACAGATCCTGTGAAAGCATTCAAGACGGGTCCCAAGTCACCCGAGCATGGGGAAGAAAACCTCATCAGTAATGAAGGTAGTGACTCCAAGCCACCCTTATCAGAGCGTGCCTCAAATTCCTCCAGTAGCATGTCATCCTCCGAGTAA

Protein Analysis

345

Amino Acids

38.62

Weight (kDa)

9.83

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20830 AT1G20830
fragaria_vesca FvH4_5g25940
malus_domestica MD06G1005200.v1.1
prunus_persica Prupe.5G006400_v2.0.a1 Prupe.5G006400_v2.0.a1 Prupe.5G006400_v2.0.a1
pyrus_communis pycom06g00490
rosa_chinensis RchiOBHm_Chr4g0410601 RchiOBHm_Chr7g0216471
rosa_laevigata RLG00000002568
rosa_multiflora Rmu_ssc0000454.1_g000026
rosa_roxburghii Rroxscaffold_3G00243210
rosa_rugosa Rorug07G0160500
rosa_samantha Rh4CG174600 Rh7AG299700 Rh7BG291200 Rh7DG301000
rosa_wichuraiana Rw7G025470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 85
Acc36I ACCTGC 3 cut(s) 85, 700, 855
AccI GTMKAC 1 cut(s) 242
AccII CGCG 1 cut(s) 637
AciI CCGC 1 cut(s) 637
AclWI GGATC 2 cut(s) 340, 890
AcsI RAATTY 4 cut(s) 337, 544, 819, 1006
AfaI GTAC 3 cut(s) 87, 500, 810
AfiI CCNNNNNNNGG 5 cut(s) 54, 108, 159, 938, 939
AgsI TTSAA 3 cut(s) 313, 514, 913
AjiI CACGTC 1 cut(s) 452
AloI GAACNNNNNNTCC 2 cut(s) 600, 632
AluBI AGCT 5 cut(s) 195, 291, 437, 460, 614
AluI AGCT 5 cut(s) 195, 291, 437, 460, 614
Alw26I GTCTC 3 cut(s) 64, 303, 649
AlwI GGATC 2 cut(s) 340, 890
AlwNI CAGNNNCTG 1 cut(s) 899
Ama87I CYCGRG 1 cut(s) 932
ApoI RAATTY 4 cut(s) 337, 544, 819, 1006
Asp700I GAANNNNTTC 1 cut(s) 908
AspS9I GGNCC 3 cut(s) 111, 465, 920
AsuC2I CCSGG 1 cut(s) 707
AsuHPI GGTGA 3 cut(s) 215, 770, 921
AsuII TTCGAA 1 cut(s) 485
AvaI CYCGRG 1 cut(s) 932
AvaII GGWCC 3 cut(s) 111, 465, 920
BaeGI GKGCMC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 103
BbsI GAAGAC 1 cut(s) 777
BccI CCATC 2 cut(s) 627, 868
BceAI ACGGC 2 cut(s) 158, 199
BciVI GTATCC 1 cut(s) 703
BclI TGATCA 1 cut(s) 285
BcnI CCSGG 1 cut(s) 707
BcoDI GTCTC 3 cut(s) 64, 303, 649
BfaI CTAG 1 cut(s) 882
BfmI CTRYAG 4 cut(s) 432, 552, 842, 891
BfuAI ACCTGC 3 cut(s) 85, 700, 855
BfuI GTATCC 1 cut(s) 703
BlpI GCTNAGC 1 cut(s) 828
Bme1390I CCNGG 1 cut(s) 707
Bme18I GGWCC 3 cut(s) 111, 465, 920
BmeT110I CYCGRG 1 cut(s) 932
BmgBI CACGTC 1 cut(s) 452
BmgT120I GGNCC 3 cut(s) 111, 465, 920
BmiI GGNNCC 4 cut(s) 112, 164, 921, 922
BmrFI CCNGG 1 cut(s) 707
BmrI ACTGGG 1 cut(s) 257
BmuI ACTGGG 1 cut(s) 257
BpiI GAAGAC 1 cut(s) 777
BplI GAGNNNNNCTC 2 cut(s) 986, 1018
BpmI CTGGAG 1 cut(s) 997
Bpu10I CCTNAGC 2 cut(s) 187, 461
Bpu1102I GCTNAGC 1 cut(s) 828
Bpu14I TTCGAA 1 cut(s) 485
BpuMI CCSGG 1 cut(s) 707
BsaI GGTCTC 2 cut(s) 64, 649
BsaJI CCNNGG 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bsc4I CCNNNNNNNGG 5 cut(s) 54, 108, 159, 938, 939
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 4 cut(s) 114, 263, 646, 1014
Bse3DI GCAATG 1 cut(s) 892
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 2 cut(s) 736, 1025
BseLI CCNNNNNNNGG 5 cut(s) 54, 108, 159, 938, 939
BseMI GCAATG 1 cut(s) 892
BseMII CTCAG 4 cut(s) 201, 306, 377, 819
BseNI ACTGG 4 cut(s) 114, 263, 646, 1014
BseRI GAGGAG 1 cut(s) 643
BseSI GKGCMC 1 cut(s) 139
Bsh1236I CGCG 1 cut(s) 637
Bsh1285I CGRYCG 1 cut(s) 124
BsiEI CGRYCG 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 932
BsiSI CCGG 2 cut(s) 707, 756
BslFI GGGAC 1 cut(s) 906
BslI CCNNNNNNNGG 5 cut(s) 54, 108, 159, 938, 939
BsmAI GTCTC 3 cut(s) 64, 303, 649
BsmFI GGGAC 1 cut(s) 906
BsmI GAATGC 1 cut(s) 908
Bso31I GGTCTC 2 cut(s) 64, 649
BsoBI CYCGRG 1 cut(s) 932
Bsp119I TTCGAA 1 cut(s) 485
Bsp1286I GDGCHC 2 cut(s) 103, 139
Bsp143I GATC 4 cut(s) 285, 332, 439, 895
Bsp1720I GCTNAGC 1 cut(s) 828
BspACI CCGC 1 cut(s) 637
BspCNI CTCAG 4 cut(s) 200, 305, 378, 820
BspFNI CGCG 1 cut(s) 637
BspLI GGNNCC 4 cut(s) 112, 164, 921, 922
BspMAI CTGCAG 1 cut(s) 846
BspMI ACCTGC 3 cut(s) 85, 700, 855
BspPI GGATC 2 cut(s) 340, 890
BspT104I TTCGAA 1 cut(s) 485
BspTNI GGTCTC 2 cut(s) 64, 649
BsrDI GCAATG 1 cut(s) 892
BsrFI RCCGGY 1 cut(s) 755
BsrI ACTGG 4 cut(s) 114, 263, 646, 1014
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 4 cut(s) 285, 332, 439, 895
BssNAI GTATAC 1 cut(s) 243
Bst1107I GTATAC 1 cut(s) 243
Bst4CI ACNGT 3 cut(s) 122, 422, 813
Bst6I CTCTTC 2 cut(s) 469, 647
BstAPI GCANNNNNTGC 1 cut(s) 752
BstBI TTCGAA 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 639, 999
BstDEI CTNAG 6 cut(s) 170, 187, 292, 386, 461, 828
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 2 cut(s) 736, 1025
BstFNI CGCG 1 cut(s) 637
BstKTI GATC 4 cut(s) 288, 335, 442, 898
BstMAI GTCTC 3 cut(s) 64, 303, 649
BstMBI GATC 4 cut(s) 285, 332, 439, 895
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 3 cut(s) 192, 752, 850
BstNSI RCATGY 1 cut(s) 1024
BstSCI CCNGG 1 cut(s) 705
BstSFI CTRYAG 4 cut(s) 432, 552, 842, 891
BstSLI GKGCMC 1 cut(s) 139
BstUI CGCG 1 cut(s) 637
BstV2I GAAGAC 1 cut(s) 777
BstX2I RGATCY 1 cut(s) 895
BstYI RGATCY 1 cut(s) 895
BstZ17I GTATAC 1 cut(s) 243
BsuI GTATCC 1 cut(s) 703
BtgI CCRYGG 1 cut(s) 277
BtrI CACGTC 1 cut(s) 452
BtsCI GGATG 2 cut(s) 736, 1025
BtsI GCAGTG 2 cut(s) 750, 839
BtsIMutI CAGTG 4 cut(s) 300, 653, 750, 839
BveI ACCTGC 3 cut(s) 85, 700, 855
Cac8I GCNNGC 2 cut(s) 639, 999
CaiI CAGNNNCTG 1 cut(s) 899
Cfr10I RCCGGY 1 cut(s) 755
Cfr13I GGNCC 3 cut(s) 111, 465, 920
Csp6I GTAC 3 cut(s) 86, 499, 809
CviAII CATG 6 cut(s) 397, 496, 803, 833, 938, 1021
CviQI GTAC 3 cut(s) 86, 499, 809
DdeI CTNAG 6 cut(s) 170, 187, 292, 386, 461, 828
DpnI GATC 4 cut(s) 287, 334, 441, 897
DpnII GATC 4 cut(s) 285, 332, 439, 895
Eam1104I CTCTTC 2 cut(s) 469, 647
EarI CTCTTC 2 cut(s) 469, 647
Eco24I GRGCYC 1 cut(s) 103
Eco31I GGTCTC 2 cut(s) 64, 649
Eco47I GGWCC 3 cut(s) 111, 465, 920
Eco88I CYCGRG 1 cut(s) 932
EcoO109I RGGNCCY 1 cut(s) 920
EcoRI GAATTC 1 cut(s) 819
EcoT38I GRGCYC 1 cut(s) 103
FaeI CATG 6 cut(s) 400, 499, 806, 836, 941, 1024
FalI AAGNNNNNCTT 2 cut(s) 971, 1003
FaqI GGGAC 1 cut(s) 906
FatI CATG 6 cut(s) 396, 495, 802, 832, 937, 1020
FauI CCCGC 1 cut(s) 630
FbaI TGATCA 1 cut(s) 285
FblI GTMKAC 1 cut(s) 242
FokI GGATG 2 cut(s) 723, 1012
FriOI GRGCYC 1 cut(s) 103
FspBI CTAG 1 cut(s) 882
GsuI CTGGAG 1 cut(s) 997
HapII CCGG 2 cut(s) 707, 756
Hin1II CATG 6 cut(s) 400, 499, 806, 836, 941, 1024
HincII GTYRAC 2 cut(s) 268, 703
HindII GTYRAC 2 cut(s) 268, 703
HinfI GANTC 5 cut(s) 16, 683, 724, 878, 974
HpaII CCGG 2 cut(s) 707, 756
HphI GGTGA 3 cut(s) 215, 770, 921
Hpy166II GTNNAC 4 cut(s) 243, 268, 703, 809
Hpy188I TCNGA 6 cut(s) 387, 449, 469, 798, 994, 1033
Hpy188III TCNNGA 4 cut(s) 43, 313, 403, 913
Hpy8I GTNNAC 4 cut(s) 243, 268, 703, 809
Hpy99I CGWCG 1 cut(s) 144
HpyAV CCTTC 4 cut(s) 49, 671, 738, 959
HpyCH4III ACNGT 3 cut(s) 122, 422, 813
HpyCH4IV ACGT 1 cut(s) 451
HpyCH4V TGCA 5 cut(s) 80, 362, 368, 746, 844
HpyF10VI GCNNNNNNNGC 3 cut(s) 192, 752, 850
HpyF3I CTNAG 6 cut(s) 170, 187, 292, 386, 461, 828
HpySE526I ACGT 1 cut(s) 451
Hsp92II CATG 6 cut(s) 400, 499, 806, 836, 941, 1024
KflI GGGWCCC 1 cut(s) 920
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 4 cut(s) 285, 332, 439, 895
MaeI CTAG 1 cut(s) 882
MaeII ACGT 1 cut(s) 451
MaeIII GTNAC 4 cut(s) 116, 758, 927, 971
MalI GATC 4 cut(s) 287, 334, 441, 897
MboI GATC 4 cut(s) 285, 332, 439, 895
MboII GAAGA 5 cut(s) 424, 486, 664, 782, 956
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 2 cut(s) 103, 139
MlyI GAGTC 3 cut(s) 10, 872, 968
MroXI GAANNNNTTC 1 cut(s) 908
MseI TTAA 2 cut(s) 528, 672
MslI CAYNNNNRTG 2 cut(s) 225, 646
MspI CCGG 2 cut(s) 707, 756
MspR9I CCNGG 1 cut(s) 707
MunI CAATTG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 908
MvnI CGCG 1 cut(s) 637
MwoI GCNNNNNNNGC 3 cut(s) 192, 752, 850
NciI CCSGG 1 cut(s) 707
NdeII GATC 4 cut(s) 285, 332, 439, 895
NlaIII CATG 6 cut(s) 400, 499, 806, 836, 941, 1024
NlaIV GGNNCC 4 cut(s) 112, 164, 921, 922
NmuCI GTSAC 3 cut(s) 758, 927, 971
NspI RCATGY 1 cut(s) 1024
NspV TTCGAA 1 cut(s) 485
PaqCI CACCTGC 1 cut(s) 85
PctI GAATGC 1 cut(s) 908
PdmI GAANNNNTTC 1 cut(s) 908
PfeI GAWTC 2 cut(s) 683, 724
PflFI GACNNNGTC 1 cut(s) 453
PleI GAGTC 3 cut(s) 10, 872, 968
PpsI GAGTC 3 cut(s) 10, 872, 968
PpuMI RGGWCCY 1 cut(s) 920
Psp5II RGGWCCY 1 cut(s) 920
PspN4I GGNNCC 4 cut(s) 112, 164, 921, 922
PspPI GGNCC 3 cut(s) 111, 465, 920
PspPPI RGGWCCY 1 cut(s) 920
PstI CTGCAG 1 cut(s) 846
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 1 cut(s) 895
PsyI GACNNNGTC 1 cut(s) 453
RsaI GTAC 3 cut(s) 87, 500, 810
RsaNI GTAC 3 cut(s) 86, 499, 809
RseI CAYNNNNRTG 2 cut(s) 225, 646
SaqAI TTAA 2 cut(s) 528, 672
Sau3AI GATC 4 cut(s) 285, 332, 439, 895
Sau96I GGNCC 3 cut(s) 111, 465, 920
SchI GAGTC 3 cut(s) 10, 872, 968
ScrFI CCNGG 1 cut(s) 707
SduI GDGCHC 2 cut(s) 103, 139
SfcI CTRYAG 4 cut(s) 432, 552, 842, 891
SfuI TTCGAA 1 cut(s) 485
SinI GGWCC 3 cut(s) 111, 465, 920
SmiMI CAYNNNNRTG 2 cut(s) 225, 646
SsiI CCGC 1 cut(s) 637
SspMI CTAG 1 cut(s) 882
StyD4I CCNGG 1 cut(s) 705
TaaI ACNGT 3 cut(s) 122, 422, 813
TaiI ACGT 1 cut(s) 454
TaqI TCGA 8 cut(s) 14, 335, 410, 485, 548, 622, 686, 817
TaqII GACCGA 1 cut(s) 673
TfiI GAWTC 2 cut(s) 683, 724
Tru1I TTAA 2 cut(s) 528, 672
Tru9I TTAA 2 cut(s) 528, 672
TscAI CASTG 4 cut(s) 300, 653, 757, 846
TseFI GTSAC 3 cut(s) 758, 927, 971
Tsp45I GTSAC 3 cut(s) 758, 927, 971
TspDTI ATGAA 5 cut(s) 118, 385, 783, 849, 978
TspGWI ACGGA 1 cut(s) 294
TspRI CASTG 4 cut(s) 300, 653, 757, 846
Tth111I GACNNNGTC 1 cut(s) 453
VpaK11BI GGWCC 3 cut(s) 111, 465, 920
XapI RAATTY 4 cut(s) 337, 544, 819, 1006
XceI RCATGY 1 cut(s) 1024
XmiI GTMKAC 1 cut(s) 242
XmnI GAANNNNTTC 1 cut(s) 908
XspI CTAG 1 cut(s) 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.