AT1G02100

leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009548
Physical Location & Seq
Forward (+)
389812 .. 392816
3005 bp
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UTR
Exon/CDS
Intron
AT1G02100.1

Sequence Viewer

Length: 999 bp
ATGGCGGAATCTCGCAGCAACAGAGCGGCGGTTCAGGCTACTAACGACGATGCATCCGCCAGTAAATTGTCTTGTGTCAAAAAGGGATATATGAAAGACGACTATGTTCATCTCTTTGTGAAAAGACCTGTTCGAAGATCTCCCATCATTAATCGAGGTTACTTTTCCCGTTGGGCTGCCTTCCGAAAGCTTATGTCTCAGTTTCTTCTAAGCGGGACAAGTTCTAAGAAACAGATACTGTCTCTCGGAGCTGGCTTTGATACTACCTATTTTCAGTTGCTGGATGAGGGGAATGGGCCCAATCTCTATGTGGAACTTGATTTTAAGGAGGTGACTAGCAAGAAGGCTGCTGTTATACAAAACTCCAGCCAACTCAGGGACAAACTAGGAGCCAATGCATCTATTTCTATTGACGAAGGACAAGTTCTCAGTGATCATTACAAGTTACTTCCAGTTGACCTGCGCGATATACCAAAATTAAGAGATGTTATATCCTTTGCAGATATGGATCTAAGTCTGCCGACGTTTATTATTGCAGAATGTGTTTTGATTTATCTGGACCCCGATTCAAGCCGTGCCATCGTCAATTGGTCGTCAAAAACGTTTTCAACTGCAGTATTTTTCTTATATGAGCAGATCCATCCAGATGATGCATTTGGGCATCAAATGATTAGAAATTTGGAGAGTCGGGGATGTGCACTCTTAAGCATTGATGCATCACCAACTTTACTTGCAAAGGAGAGATTGTTTCTTGATAATGGATGGCAGAGAGCTGTTGCCTGGGACATGCTAAAAGTGTATGGTAGTTTTGTTGATACTCAAGAAAAACGCAGGATCGAGCGATTGGAGTTGTTTGACGAATTTGAAGAGTGGCACATGATGCAGGAACATTACTGTGTCACATATGCTGTCAATGATGCAATGGGAATATTTGGTGATTTCGGTTTCACAAGAGAAGGGGGCGGTGAAAGAATGAGCTCATCAGCGTCATCACCTTGA

Protein Analysis

332

Amino Acids

37.55

Weight (kDa)

5.76

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 35 - 197 6.1e-19 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 468
AccBSI CCGCTC 1 cut(s) 26
AccII CGCG 1 cut(s) 465
AciI CCGC 6 cut(s) 5, 26, 29, 57, 213, 963
AclI AACGTT 1 cut(s) 602
AclWI GGATC 3 cut(s) 516, 631, 842
AcsI RAATTY 2 cut(s) 676, 860
AfiI CCNNNNNNNGG 1 cut(s) 376
AflII CTTAAG 1 cut(s) 703
AgsI TTSAA 3 cut(s) 570, 609, 866
AjnI CCWGG 1 cut(s) 779
AluBI AGCT 4 cut(s) 190, 251, 773, 978
AluI AGCT 4 cut(s) 190, 251, 773, 978
Alw21I GWGCWC 2 cut(s) 700, 980
Alw26I GTCTC 2 cut(s) 201, 246
Alw44I GTGCAC 1 cut(s) 696
AlwI GGATC 3 cut(s) 516, 631, 842
AlwNI CAGNNNCTG 2 cut(s) 238, 280
AoxI GGCC 1 cut(s) 296
ApaI GGGCCC 1 cut(s) 300
ApaLI GTGCAC 1 cut(s) 696
ApeKI GCWGC 3 cut(s) 15, 176, 347
ApoI RAATTY 2 cut(s) 676, 860
AseI ATTAAT 1 cut(s) 150
AspLEI GCGC 1 cut(s) 465
AspS9I GGNCC 3 cut(s) 296, 297, 559
AsuHPI GGTGA 5 cut(s) 343, 711, 947, 977, 984
AsuII TTCGAA 1 cut(s) 133
AvaII GGWCC 1 cut(s) 559
BaeGI GKGCMC 2 cut(s) 300, 700
BanII GRGCYC 2 cut(s) 300, 980
Bbv12I GWGCWC 2 cut(s) 700, 980
BbvI GCAGC 3 cut(s) 27, 163, 334
BccI CCATC 4 cut(s) 152, 587, 648, 756
BceAI ACGGC 1 cut(s) 558
BciT130I CCWGG 1 cut(s) 781
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 2 cut(s) 201, 246
BfaI CTAG 2 cut(s) 336, 386
BfmI CTRYAG 1 cut(s) 612
BfrI CTTAAG 1 cut(s) 703
BfuAI ACCTGC 1 cut(s) 468
BglII AGATCT 1 cut(s) 137
BisI GCNGC 4 cut(s) 16, 27, 177, 348
BlsI GCNGC 4 cut(s) 17, 28, 178, 349
Bme1390I CCNGG 1 cut(s) 781
Bme18I GGWCC 1 cut(s) 559
BmgT120I GGNCC 3 cut(s) 296, 297, 559
BmiI GGNNCC 3 cut(s) 298, 391, 561
BmrFI CCNGG 1 cut(s) 781
BmsI GCATC 9 cut(s) 40, 62, 407, 640, 670, 703, 725, 870, 907
BpmI CTGGAG 1 cut(s) 349
Bpu14I TTCGAA 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 804
BsaBI GATNNNNATC 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 780
BsaXI ACNNNNNCTCC 2 cut(s) 731, 761
Bsc4I CCNNNNNNNGG 1 cut(s) 376
Bse1I ACTGG 2 cut(s) 60, 452
Bse3DI GCAATG 1 cut(s) 927
Bse8I GATNNNNATC 1 cut(s) 507
BseBI CCWGG 1 cut(s) 781
BseDI CCNNGG 1 cut(s) 780
BseGI GGATG 5 cut(s) 53, 289, 640, 698, 767
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 1 cut(s) 376
BseMI GCAATG 1 cut(s) 927
BseMII CTCAG 3 cut(s) 212, 388, 442
BseNI ACTGG 2 cut(s) 60, 452
BseSI GKGCMC 2 cut(s) 300, 700
BseXI GCAGC 3 cut(s) 27, 163, 334
Bsh1236I CGCG 1 cut(s) 465
BshFI GGCC 1 cut(s) 298
BsiHKAI GWGCWC 2 cut(s) 700, 980
BslFI GGGAC 3 cut(s) 229, 392, 797
BslI CCNNNNNNNGG 1 cut(s) 376
BsmAI GTCTC 2 cut(s) 201, 246
BsmFI GGGAC 3 cut(s) 229, 392, 797
BsnI GGCC 1 cut(s) 298
Bsp119I TTCGAA 1 cut(s) 133
Bsp120I GGGCCC 1 cut(s) 296
Bsp1286I GDGCHC 3 cut(s) 300, 700, 980
Bsp143I GATC 5 cut(s) 137, 433, 508, 636, 834
BspACI CCGC 6 cut(s) 5, 26, 29, 57, 213, 963
BspANI GGCC 1 cut(s) 298
BspCNI CTCAG 3 cut(s) 211, 387, 441
BspFNI CGCG 1 cut(s) 465
BspLI GGNNCC 3 cut(s) 298, 391, 561
BspMAI CTGCAG 1 cut(s) 616
BspMI ACCTGC 1 cut(s) 468
BspPI GGATC 3 cut(s) 516, 631, 842
BspT104I TTCGAA 1 cut(s) 133
BspTI CTTAAG 1 cut(s) 703
BsrBI CCGCTC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 927
BsrI ACTGG 2 cut(s) 60, 452
BssECI CCNNGG 1 cut(s) 780
BssMI GATC 5 cut(s) 137, 433, 508, 636, 834
Bst2UI CCWGG 1 cut(s) 781
Bst4CI ACNGT 2 cut(s) 240, 896
Bst6I CTCTTC 1 cut(s) 861
BstAFI CTTAAG 1 cut(s) 703
BstAPI GCANNNNNTGC 1 cut(s) 880
BstBI TTCGAA 1 cut(s) 133
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 6 cut(s) 198, 209, 225, 374, 428, 512
BstF5I GGATG 5 cut(s) 53, 289, 640, 698, 767
BstFNI CGCG 1 cut(s) 465
BstHHI GCGC 1 cut(s) 465
BstKTI GATC 5 cut(s) 140, 436, 511, 639, 837
BstMAI GTCTC 2 cut(s) 201, 246
BstMBI GATC 5 cut(s) 137, 433, 508, 636, 834
BstMWI GCNNNNNNNGC 2 cut(s) 35, 880
BstNI CCWGG 1 cut(s) 781
BstNSI RCATGY 1 cut(s) 790
BstSCI CCNGG 1 cut(s) 779
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 2 cut(s) 300, 700
BstUI CGCG 1 cut(s) 465
BstV1I GCAGC 3 cut(s) 27, 163, 334
BstX2I RGATCY 3 cut(s) 137, 508, 636
BstYI RGATCY 3 cut(s) 137, 508, 636
BsuRI GGCC 1 cut(s) 298
BtsCI GGATG 5 cut(s) 53, 289, 640, 698, 767
BtsIMutI CAGTG 1 cut(s) 436
BveI ACCTGC 1 cut(s) 468
Cac8I GCNNGC 1 cut(s) 253
CaiI CAGNNNCTG 2 cut(s) 238, 280
CfoI GCGC 1 cut(s) 465
Cfr13I GGNCC 3 cut(s) 296, 297, 559
CseI GACGC 1 cut(s) 975
CviAII CATG 2 cut(s) 787, 877
DdeI CTNAG 6 cut(s) 198, 209, 225, 374, 428, 512
DpnI GATC 5 cut(s) 139, 435, 510, 638, 836
DpnII GATC 5 cut(s) 137, 433, 508, 636, 834
Eam1104I CTCTTC 1 cut(s) 861
EarI CTCTTC 1 cut(s) 861
EciI GGCGGA 2 cut(s) 20, 46
Ecl136II GAGCTC 1 cut(s) 978
Eco24I GRGCYC 2 cut(s) 300, 980
Eco47I GGWCC 1 cut(s) 559
Eco53kI GAGCTC 1 cut(s) 978
EcoICRI GAGCTC 1 cut(s) 978
EcoRII CCWGG 1 cut(s) 779
EcoT22I ATGCAT 4 cut(s) 55, 400, 655, 718
EcoT38I GRGCYC 2 cut(s) 300, 980
FaeI CATG 2 cut(s) 790, 880
FaqI GGGAC 3 cut(s) 229, 392, 797
FatI CATG 2 cut(s) 786, 876
FauI CCCGC 1 cut(s) 206
FauNDI CATATG 1 cut(s) 904
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 4 cut(s) 16, 27, 177, 348
FokI GGATG 5 cut(s) 40, 296, 627, 705, 774
FriOI GRGCYC 2 cut(s) 300, 980
Fsp4HI GCNGC 4 cut(s) 16, 27, 177, 348
FspBI CTAG 2 cut(s) 336, 386
GlaI GCGC 1 cut(s) 464
GluI GCNGC 4 cut(s) 16, 27, 177, 348
GsuI CTGGAG 1 cut(s) 349
HaeIII GGCC 1 cut(s) 298
HgaI GACGC 1 cut(s) 975
HhaI GCGC 1 cut(s) 465
Hin1II CATG 2 cut(s) 790, 880
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 3 cut(s) 8, 566, 685
HphI GGTGA 5 cut(s) 343, 711, 947, 977, 984
Hpy166II GTNNAC 2 cut(s) 457, 698
Hpy188I TCNGA 2 cut(s) 185, 248
Hpy188III TCNNGA 4 cut(s) 557, 644, 752, 821
Hpy8I GTNNAC 2 cut(s) 457, 698
Hpy99I CGWCG 2 cut(s) 50, 526
HpyAV CCTTC 4 cut(s) 190, 337, 410, 950
HpyCH4III ACNGT 2 cut(s) 240, 896
HpyCH4IV ACGT 2 cut(s) 524, 602
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 880
HpyF3I CTNAG 6 cut(s) 198, 209, 225, 374, 428, 512
HpySE526I ACGT 2 cut(s) 524, 602
Hsp92II CATG 2 cut(s) 790, 880
HspAI GCGC 1 cut(s) 463
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 5 cut(s) 137, 433, 508, 636, 834
LmnI GCTCC 2 cut(s) 248, 389
Lsp1109I GCAGC 3 cut(s) 27, 163, 334
LweI GCATC 9 cut(s) 40, 62, 407, 640, 670, 703, 725, 870, 907
MaeI CTAG 2 cut(s) 336, 386
MaeII ACGT 2 cut(s) 524, 602
MaeIII GTNAC 4 cut(s) 158, 331, 444, 898
MalI GATC 5 cut(s) 139, 435, 510, 638, 836
MbiI CCGCTC 1 cut(s) 26
MboI GATC 5 cut(s) 137, 433, 508, 636, 834
MboII GAAGA 3 cut(s) 147, 197, 878
MfeI CAATTG 1 cut(s) 586
MflI RGATCY 3 cut(s) 137, 508, 636
MhlI GDGCHC 3 cut(s) 300, 700, 980
MluCI AATT 5 cut(s) 65, 476, 586, 676, 860
MlyI GAGTC 1 cut(s) 694
MnlI CCTC 3 cut(s) 149, 280, 322
Mph1103I ATGCAT 4 cut(s) 55, 400, 655, 718
MseI TTAA 4 cut(s) 150, 324, 479, 704
MslI CAYNNNNRTG 2 cut(s) 645, 894
MspCI CTTAAG 1 cut(s) 703
MspR9I CCNGG 1 cut(s) 781
MunI CAATTG 1 cut(s) 586
MvaI CCWGG 1 cut(s) 781
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 2 cut(s) 35, 880
NdeI CATATG 1 cut(s) 904
NdeII GATC 5 cut(s) 137, 433, 508, 636, 834
NlaIII CATG 2 cut(s) 790, 880
NlaIV GGNNCC 3 cut(s) 298, 391, 561
NmuCI GTSAC 2 cut(s) 331, 898
NsiI ATGCAT 4 cut(s) 55, 400, 655, 718
NspI RCATGY 1 cut(s) 790
NspV TTCGAA 1 cut(s) 133
PcsI WCGNNNNNNNCGW 1 cut(s) 599
PfeI GAWTC 2 cut(s) 8, 566
PkrI GCNGC 4 cut(s) 17, 28, 178, 349
PleI GAGTC 1 cut(s) 693
PpsI GAGTC 1 cut(s) 693
PshBI ATTAAT 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 980
Psp1406I AACGTT 1 cut(s) 602
Psp6I CCWGG 1 cut(s) 779
PspGI CCWGG 1 cut(s) 779
PspN4I GGNNCC 3 cut(s) 298, 391, 561
PspOMI GGGCCC 1 cut(s) 296
PspPI GGNCC 3 cut(s) 296, 297, 559
PstI CTGCAG 1 cut(s) 616
PstNI CAGNNNCTG 2 cut(s) 238, 280
PsuI RGATCY 3 cut(s) 137, 508, 636
RseI CAYNNNNRTG 2 cut(s) 645, 894
SacI GAGCTC 1 cut(s) 980
SaqAI TTAA 4 cut(s) 150, 324, 479, 704
SatI GCNGC 4 cut(s) 16, 27, 177, 348
Sau3AI GATC 5 cut(s) 137, 433, 508, 636, 834
Sau96I GGNCC 3 cut(s) 296, 297, 559
SchI GAGTC 1 cut(s) 694
ScrFI CCNGG 1 cut(s) 781
SduI GDGCHC 3 cut(s) 300, 700, 980
SfaNI GCATC 9 cut(s) 40, 62, 407, 640, 670, 703, 725, 870, 907
SfcI CTRYAG 1 cut(s) 612
SfuI TTCGAA 1 cut(s) 133
SinI GGWCC 1 cut(s) 559
SmiMI CAYNNNNRTG 2 cut(s) 645, 894
SmlI CTYRAG 2 cut(s) 703, 819
SmoI CTYRAG 2 cut(s) 703, 819
Sse9I AATT 5 cut(s) 65, 476, 586, 676, 860
SsiI CCGC 6 cut(s) 5, 26, 29, 57, 213, 963
SspI AATATT 1 cut(s) 930
SspMI CTAG 2 cut(s) 336, 386
SstI GAGCTC 1 cut(s) 980
StyD4I CCNGG 1 cut(s) 779
TaaI ACNGT 2 cut(s) 240, 896
TaiI ACGT 2 cut(s) 527, 605
TaqI TCGA 3 cut(s) 133, 154, 837
TasI AATT 5 cut(s) 65, 476, 586, 676, 860
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 2 cut(s) 8, 566
Tru1I TTAA 4 cut(s) 150, 324, 479, 704
Tru9I TTAA 4 cut(s) 150, 324, 479, 704
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 2 cut(s) 331, 898
TseI GCWGC 3 cut(s) 15, 176, 347
Tsp45I GTSAC 2 cut(s) 331, 898
TspDTI ATGAA 2 cut(s) 98, 107
TspRI CASTG 1 cut(s) 436
Vha464I CTTAAG 1 cut(s) 703
VneI GTGCAC 1 cut(s) 696
VpaK11BI GGWCC 1 cut(s) 559
VspI ATTAAT 1 cut(s) 150
XapI RAATTY 2 cut(s) 676, 860
XceI RCATGY 1 cut(s) 790
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XspI CTAG 2 cut(s) 336, 386
Zsp2I ATGCAT 4 cut(s) 55, 400, 655, 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.