MD02G1211800.v1.1

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009548
Physical Location & Seq
Forward (+)
22663038 .. 22667000
3963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1211800.v1.1.491

Sequence Viewer

Length: 807 bp
ATGGATTCGCACAGCAACAAAGAAGCAGTTCAAGCAACAAACGACGACGCCTCGGCCAGCAAACTGTCGTGCGTGAAGAAAGGGTACATGAAAGACGATTATGTCCATTTGTTTGTGAGAAGACCTGTGAGAAGATCTCCGATTATTAACCGCGGTTACTTTGCACGTTGGGCCGCTCTGCGGAAGCTTCTAAATCAGTTTCTTGATGCCGGAGGAAATGGTGGCGAAAAGGGTCATTTGAAGAAGCAGATATTATCACTTGGAGCTGGCTTTGATACAACATATTTTCAGCTGCAGGATGAGGGGAGAGCACCATATTTATATGTTGAGCTGGATTTTAAGGAGGTAACTAGTAAGAAGGCCACACTTATTGAAACGTGCAGTCATTTGAGGGACAAAATTAGTGCAACAGCATCAATCTCTCGAGAAAGTGGAGAAGTGCTCAGTGACCACTACAAGCTACTTCCTGTTGATTTGCGTGACATACCAAAACTAGACAATGTTATAGCTTTAGCTGGTATGAATCCGAGCCTTCCAACTTTTATAATTGCAGAATGCGTTTTGATATACTTGGATCCAGATTCAAGTCGTTCCATAGTTAGATGGACAGCAAAAGCATTTTCTACAGCGGTATTTTTCTTATATGAGCAGATTCATCCGGATGATGCTTTTGGGCAGCAGATGATCAGAAACTTGGAGAGTCGAGGCTGTGCGCTCTTGGGCATACATGATACACCAACATTACAGGCAAAGGAAAACCTTTTTGTTGACCAAGGATGGCAGGTAGGTGTTGTAAATTATATTTAG

Protein Analysis

269

Amino Acids

30.03

Weight (kDa)

7.05

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 39 - 203 2.5e-20 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
AasI GACNNNNNNGTC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 772
AccBSI CCGCTC 1 cut(s) 176
AccII CGCG 1 cut(s) 153
AccIII TCCGGA 1 cut(s) 658
AciI CCGC 5 cut(s) 151, 153, 174, 181, 629
AclWI GGATC 2 cut(s) 569, 582
AcoI YGGCCR 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 180
AgsI TTSAA 4 cut(s) 32, 241, 374, 585
AhlI ACTAGT 1 cut(s) 350
AluBI AGCT 7 cut(s) 187, 266, 292, 331, 460, 509, 515
AluI AGCT 7 cut(s) 187, 266, 292, 331, 460, 509, 515
Alw21I GWGCWC 2 cut(s) 313, 444
AlwI GGATC 2 cut(s) 569, 582
Ama87I CYCGRG 1 cut(s) 423
Aor13HI TCCGGA 1 cut(s) 658
AoxI GGCC 3 cut(s) 54, 171, 360
ApeKI GCWGC 2 cut(s) 292, 676
Asp700I GAANNNNTTC 1 cut(s) 27
AspLEI GCGC 1 cut(s) 715
AspS9I GGNCC 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 423
BamHI GGATCC 1 cut(s) 574
BarI GAAGNNNNNNTAC 2 cut(s) 68, 100
BbsI GAAGAC 1 cut(s) 127
Bbv12I GWGCWC 2 cut(s) 313, 444
BbvI GCAGC 2 cut(s) 279, 688
BccI CCATC 2 cut(s) 597, 771
BclI TGATCA 1 cut(s) 684
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 2 cut(s) 351, 494
BfmI CTRYAG 2 cut(s) 293, 624
BfuAI ACCTGC 1 cut(s) 772
BglII AGATCT 1 cut(s) 134
BisI GCNGC 3 cut(s) 174, 293, 677
BlsI GCNGC 3 cut(s) 175, 294, 678
BmeT110I CYCGRG 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 171
BmiI GGNNCC 1 cut(s) 576
BmsI GCATC 3 cut(s) 196, 422, 655
BpiI GAAGAC 1 cut(s) 127
BplI GAGNNNNNCTC 4 cut(s) 121, 153, 426, 458
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 3 cut(s) 51, 151, 772
BsaWI WCCGGW 1 cut(s) 658
Bsc4I CCNNNNNNNGG 1 cut(s) 180
BseAI TCCGGA 1 cut(s) 658
BseDI CCNNGG 3 cut(s) 51, 151, 772
BseGI GGATG 4 cut(s) 304, 655, 667, 782
BseLI CCNNNNNNNGG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 457
BseXI GCAGC 2 cut(s) 279, 688
BsgI GTGCAG 1 cut(s) 400
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 3 cut(s) 56, 173, 362
BsiHKAI GWGCWC 2 cut(s) 313, 444
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 2 cut(s) 210, 659
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 1 cut(s) 180
BsmFI GGGAC 1 cut(s) 407
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 3 cut(s) 56, 173, 362
BsoBI CYCGRG 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 313, 444
Bsp13I TCCGGA 1 cut(s) 658
Bsp143I GATC 3 cut(s) 134, 574, 684
BspACI CCGC 5 cut(s) 151, 153, 174, 181, 629
BspANI GGCC 3 cut(s) 56, 173, 362
BspCNI CTCAG 1 cut(s) 456
BspEI TCCGGA 1 cut(s) 658
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 576
BspMAI CTGCAG 1 cut(s) 297
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 2 cut(s) 569, 582
BsrBI CCGCTC 1 cut(s) 176
BssECI CCNNGG 3 cut(s) 51, 151, 772
BssMI GATC 3 cut(s) 134, 574, 684
BssNI GRCGYC 1 cut(s) 48
BssT1I CCWWGG 1 cut(s) 772
Bst4CI ACNGT 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 2 cut(s) 58, 268
BstDEI CTNAG 1 cut(s) 443
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 4 cut(s) 304, 655, 667, 782
BstFNI CGCG 1 cut(s) 153
BstHHI GCGC 1 cut(s) 715
BstKTI GATC 3 cut(s) 137, 577, 687
BstMBI GATC 3 cut(s) 134, 574, 684
BstMWI GCNNNNNNNGC 2 cut(s) 32, 170
BstSFI CTRYAG 2 cut(s) 293, 624
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 2 cut(s) 279, 688
BstV2I GAAGAC 1 cut(s) 127
BstX2I RGATCY 2 cut(s) 134, 574
BstYI RGATCY 2 cut(s) 134, 574
BsuRI GGCC 3 cut(s) 56, 173, 362
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 4 cut(s) 304, 655, 667, 782
BtsIMutI CAGTG 1 cut(s) 451
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 2 cut(s) 58, 268
CfoI GCGC 1 cut(s) 715
Cfr13I GGNCC 1 cut(s) 171
Cfr42I CCGCGG 1 cut(s) 154
CseI GACGC 1 cut(s) 56
Csp6I GTAC 1 cut(s) 85
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 2 cut(s) 88, 728
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 1 cut(s) 443
DpnI GATC 3 cut(s) 136, 576, 686
DpnII GATC 3 cut(s) 134, 574, 684
DrdI GACNNNNNNGTC 1 cut(s) 101
DseDI GACNNNNNNGTC 1 cut(s) 101
EaeI YGGCCR 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 772
Eco88I CYCGRG 1 cut(s) 423
EcoT14I CCWWGG 1 cut(s) 772
ErhI CCWWGG 1 cut(s) 772
FaeI CATG 2 cut(s) 91, 731
FaqI GGGAC 1 cut(s) 407
FatI CATG 2 cut(s) 87, 727
FbaI TGATCA 1 cut(s) 684
Fnu4HI GCNGC 3 cut(s) 174, 293, 677
FokI GGATG 4 cut(s) 311, 642, 674, 789
Fsp4HI GCNGC 3 cut(s) 174, 293, 677
FspBI CTAG 2 cut(s) 351, 494
GlaI GCGC 1 cut(s) 714
GluI GCNGC 3 cut(s) 174, 293, 677
HaeIII GGCC 3 cut(s) 56, 173, 362
HapII CCGG 2 cut(s) 210, 659
HgaI GACGC 1 cut(s) 56
HhaI GCGC 1 cut(s) 715
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 2 cut(s) 91, 731
Hin6I GCGC 1 cut(s) 713
HinP1I GCGC 1 cut(s) 713
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 5 cut(s) 5, 523, 581, 652, 700
HpaII CCGG 2 cut(s) 210, 659
Hpy166II GTNNAC 1 cut(s) 769
Hpy188I TCNGA 3 cut(s) 141, 528, 689
Hpy188III TCNNGA 5 cut(s) 203, 423, 425, 578, 659
Hpy8I GTNNAC 1 cut(s) 769
Hpy99I CGWCG 2 cut(s) 47, 50
HpyAV CCTTC 2 cut(s) 352, 542
HpyCH4III ACNGT 1 cut(s) 66
HpyCH4IV ACGT 2 cut(s) 166, 377
HpyCH4V TGCA 5 cut(s) 164, 295, 381, 407, 551
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 170
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 2 cut(s) 166, 377
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 2 cut(s) 91, 731
HspAI GCGC 1 cut(s) 713
Kpn2I TCCGGA 1 cut(s) 658
Ksp22I TGATCA 1 cut(s) 684
KspI CCGCGG 1 cut(s) 154
Kzo9I GATC 3 cut(s) 134, 574, 684
LmnI GCTCC 1 cut(s) 263
Lsp1109I GCAGC 2 cut(s) 279, 688
LweI GCATC 3 cut(s) 196, 422, 655
MaeI CTAG 2 cut(s) 351, 494
MaeII ACGT 2 cut(s) 166, 377
MaeIII GTNAC 4 cut(s) 155, 346, 446, 479
MalI GATC 3 cut(s) 136, 576, 686
MbiI CCGCTC 1 cut(s) 176
MboI GATC 3 cut(s) 134, 574, 684
MboII GAAGA 4 cut(s) 88, 132, 144, 253
MflI RGATCY 2 cut(s) 134, 574
MhlI GDGCHC 2 cut(s) 313, 444
MluCI AATT 3 cut(s) 399, 546, 796
MlyI GAGTC 1 cut(s) 709
MmeI TCCRAC 1 cut(s) 560
MnlI CCTC 6 cut(s) 61, 206, 295, 337, 384, 698
MroI TCCGGA 1 cut(s) 658
MroXI GAANNNNTTC 1 cut(s) 27
MseI TTAA 2 cut(s) 147, 339
MslI CAYNNNNRTG 1 cut(s) 660
MspA1I CMGCKG 3 cut(s) 153, 292, 629
MspI CCGG 2 cut(s) 210, 659
Mva1269I GAATGC 1 cut(s) 560
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 2 cut(s) 32, 170
NdeII GATC 3 cut(s) 134, 574, 684
NlaIII CATG 2 cut(s) 91, 731
NlaIV GGNNCC 1 cut(s) 576
NmeAIII GCCGAG 1 cut(s) 32
NmuCI GTSAC 2 cut(s) 446, 479
PaeR7I CTCGAG 1 cut(s) 423
PctI GAATGC 1 cut(s) 560
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 4 cut(s) 5, 523, 581, 652
PkrI GCNGC 3 cut(s) 175, 294, 678
PleI GAGTC 1 cut(s) 708
PpsI GAGTC 1 cut(s) 708
PsiI TTATAA 1 cut(s) 545
PspN4I GGNNCC 1 cut(s) 576
PspPI GGNCC 1 cut(s) 171
PstI CTGCAG 1 cut(s) 297
PsuI RGATCY 2 cut(s) 134, 574
PvuII CAGCTG 1 cut(s) 292
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
RseI CAYNNNNRTG 1 cut(s) 660
SacII CCGCGG 1 cut(s) 154
SaqAI TTAA 2 cut(s) 147, 339
SatI GCNGC 3 cut(s) 174, 293, 677
Sau3AI GATC 3 cut(s) 134, 574, 684
Sau96I GGNCC 1 cut(s) 171
SchI GAGTC 1 cut(s) 709
SduI GDGCHC 2 cut(s) 313, 444
SfaNI GCATC 3 cut(s) 196, 422, 655
SfcI CTRYAG 2 cut(s) 293, 624
Sfr274I CTCGAG 1 cut(s) 423
Sfr303I CCGCGG 1 cut(s) 154
SgrBI CCGCGG 1 cut(s) 154
SlaI CTCGAG 1 cut(s) 423
SmiMI CAYNNNNRTG 1 cut(s) 660
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
SpeI ACTAGT 1 cut(s) 350
Sse9I AATT 3 cut(s) 399, 546, 796
SsiI CCGC 5 cut(s) 151, 153, 174, 181, 629
SspMI CTAG 2 cut(s) 351, 494
StyI CCWWGG 1 cut(s) 772
TaaI ACNGT 1 cut(s) 66
TaiI ACGT 2 cut(s) 169, 380
TaqI TCGA 2 cut(s) 424, 703
TasI AATT 3 cut(s) 399, 546, 796
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 4 cut(s) 5, 523, 581, 652
Tru1I TTAA 2 cut(s) 147, 339
Tru9I TTAA 2 cut(s) 147, 339
TscAI CASTG 1 cut(s) 451
TseFI GTSAC 2 cut(s) 446, 479
TseI GCWGC 2 cut(s) 292, 676
Tsp45I GTSAC 2 cut(s) 446, 479
TspDTI ATGAA 3 cut(s) 104, 536, 644
TspRI CASTG 1 cut(s) 451
XhoI CTCGAG 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 2 cut(s) 351, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.