Rw1G017320

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009548
Physical Location & Seq
Forward (+)
38394872 .. 38401457
6586 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G017320.1

Sequence Viewer

Length: 942 bp
ATGGCGAAGCCAGTGGCTGATTGGCAGAGCAACAAGGAAGCAGTTCAGGCCACAAACGACGACGCTTCCGCCAGCAAACTGTCCTGCGTGAAAAAGGGTTACATGAAAGACGATTATGTCCATTTGTTTGTGAGAAGACCTGTCAGAAGATCACCCATTATTAACCGCGGTTACTTTGCCCGCTGGGCTGCGCTTCGCAAACTTATGAATCAGTTTCTTGATACTGAAGTCGGCGAAAAGGGTGGGTTGAAGAAGCAGATACTATCACTTGGAGCTGGCTTCGATACCACATATTTCCAATTGCAGGATGAAAGGAGAGCACCATATTTATATGTGGAGTTGGATTTTAAGGAGGTTACTAGAAAGAAGGCAACAATTATTGAAACCTGCAGTCAAATGAGGGACAAAATTAGTGCAACAGCATCAATATCTCGAGAGAGAGGAGAAGTGCTCAGTGACTGCTATAAGCTACTTCCAGTTGATTTGCGGGACATACATCAATTAAACGAGGTCATAGCTTTGGCTGGTATGGATCCCAGCCTGCCAACTTTTATAATTGCAGAATGCGTTTTGATATACATTGATCCAGAATCCAGTCGTGCCATAGTTGGCTGGGCATCAAAAACATTTTCAACTGCAATTTTTTTCTTATATGAGCAGATTCATCCAGATGATGCTTTTGGGCAGCAGATGATCAGAAACTTGGAGAGTCGAGGTTGTTCGCTCTTGGGAATACATGATACGCCAACATTGCAGGCAAAGGAAAGACTTTTTCTTGATCGAGGATGGCAGAGAGCTGTTGCCTGGGACATGCTGAGAGTATATAGTAGTTTTGTTGAAGCTCAAGAAAAACGCAGGATTGAACGATTGGAATTGTTTGATGAATTTGAAGAATGGCACATGATGCAGGCAAGTCAACTCCTCCTTTCTGTCGTAAAATAA

Protein Analysis

313

Amino Acids

36.04

Weight (kDa)

6.97

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 44 - 204 4.3e-19 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AasI GACNNNNNNGTC 1 cut(s) 116
Acc36I ACCTGC 1 cut(s) 395
AccII CGCG 1 cut(s) 168
AciI CCGC 5 cut(s) 69, 166, 168, 181, 487
AclWI GGATC 3 cut(s) 527, 540, 578
AcsI RAATTY 1 cut(s) 884
AcuI CTGAAG 1 cut(s) 246
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 6 cut(s) 250, 383, 633, 839, 863, 890
AjnI CCWGG 1 cut(s) 803
AluBI AGCT 5 cut(s) 275, 469, 518, 797, 842
AluI AGCT 5 cut(s) 275, 469, 518, 797, 842
Alw21I GWGCWC 2 cut(s) 322, 453
AlwI GGATC 3 cut(s) 527, 540, 578
AlwNI CAGNNNCTG 2 cut(s) 17, 459
Ama87I CYCGRG 1 cut(s) 432
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 2 cut(s) 188, 685
ApoI RAATTY 1 cut(s) 884
Asp700I GAANNNNTTC 1 cut(s) 42
AspLEI GCGC 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 144
AvaI CYCGRG 1 cut(s) 432
BaeI ACNNNNGTAYC 2 cut(s) 213, 246
BamHI GGATCC 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 142
Bbv12I GWGCWC 2 cut(s) 322, 453
BbvI GCAGC 2 cut(s) 175, 697
BccI CCATC 1 cut(s) 780
BciT130I CCWGG 1 cut(s) 805
BclI TGATCA 1 cut(s) 693
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 388
BfuAI ACCTGC 1 cut(s) 395
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 2 cut(s) 189, 686
BlsI GCNGC 2 cut(s) 190, 687
Bme1390I CCNGG 1 cut(s) 805
BmeT110I CYCGRG 1 cut(s) 432
BmiI GGNNCC 1 cut(s) 534
BmrFI CCNGG 1 cut(s) 805
BmsI GCATC 4 cut(s) 431, 626, 664, 894
BpiI GAAGAC 1 cut(s) 142
BplI GAGNNNNNCTC 2 cut(s) 435, 467
BpuEI CTTGAG 1 cut(s) 828
BsaJI CCNNGG 2 cut(s) 166, 804
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse1I ACTGG 3 cut(s) 11, 476, 594
Bse3DI GCAATG 1 cut(s) 749
BseBI CCWGG 1 cut(s) 805
BseDI CCNNGG 2 cut(s) 166, 804
BseGI GGATG 3 cut(s) 313, 664, 791
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMI GCAATG 1 cut(s) 749
BseMII CTCAG 2 cut(s) 466, 806
BseNI ACTGG 3 cut(s) 11, 476, 594
BseRI GAGGAG 2 cut(s) 456, 911
BseXI GCAGC 2 cut(s) 175, 697
BseYI CCCAGC 3 cut(s) 183, 536, 612
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 1 cut(s) 50
BsiHKAI GWGCWC 2 cut(s) 322, 453
BsiHKCI CYCGRG 1 cut(s) 432
BslFI GGGAC 3 cut(s) 416, 503, 821
BslI CCNNNNNNNGG 1 cut(s) 304
BsmFI GGGAC 3 cut(s) 416, 503, 821
BsmI GAATGC 1 cut(s) 569
BsnI GGCC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 432
Bsp1286I GDGCHC 2 cut(s) 322, 453
Bsp143I GATC 5 cut(s) 149, 532, 583, 693, 778
BspACI CCGC 5 cut(s) 69, 166, 168, 181, 487
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 2 cut(s) 465, 807
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 534
BspMAI CTGCAG 1 cut(s) 392
BspMI ACCTGC 1 cut(s) 395
BspPI GGATC 3 cut(s) 527, 540, 578
BsrDI GCAATG 1 cut(s) 749
BsrI ACTGG 3 cut(s) 11, 476, 594
BssECI CCNNGG 2 cut(s) 166, 804
BssMI GATC 5 cut(s) 149, 532, 583, 693, 778
Bst2UI CCWGG 1 cut(s) 805
Bst4CI ACNGT 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 904
BstC8I GCNNGC 6 cut(s) 73, 181, 277, 542, 756, 909
BstDEI CTNAG 2 cut(s) 452, 815
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 3 cut(s) 313, 664, 791
BstFNI CGCG 1 cut(s) 168
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 5 cut(s) 152, 535, 586, 696, 781
BstMBI GATC 5 cut(s) 149, 532, 583, 693, 778
BstMWI GCNNNNNNNGC 4 cut(s) 47, 185, 751, 904
BstNI CCWGG 1 cut(s) 805
BstNSI RCATGY 1 cut(s) 814
BstSCI CCNGG 1 cut(s) 803
BstSFI CTRYAG 1 cut(s) 388
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 175, 697
BstV2I GAAGAC 1 cut(s) 142
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 3 cut(s) 313, 664, 791
BtsIMutI CAGTG 2 cut(s) 18, 460
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 6 cut(s) 73, 181, 277, 542, 756, 909
CaiI CAGNNNCTG 2 cut(s) 17, 459
CfoI GCGC 1 cut(s) 193
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 1 cut(s) 71
CviAII CATG 4 cut(s) 103, 737, 811, 901
DdeI CTNAG 2 cut(s) 452, 815
DpnI GATC 5 cut(s) 151, 534, 585, 695, 780
DpnII GATC 5 cut(s) 149, 532, 583, 693, 778
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EciI GGCGGA 1 cut(s) 58
Eco57I CTGAAG 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 432
EcoRII CCWGG 1 cut(s) 803
FaeI CATG 4 cut(s) 106, 740, 814, 904
FaqI GGGAC 3 cut(s) 416, 503, 821
FatI CATG 4 cut(s) 102, 736, 810, 900
FauI CCCGC 2 cut(s) 188, 480
FbaI TGATCA 1 cut(s) 693
Fnu4HI GCNGC 2 cut(s) 189, 686
FokI GGATG 3 cut(s) 320, 651, 798
Fsp4HI GCNGC 2 cut(s) 189, 686
FspBI CTAG 1 cut(s) 360
GlaI GCGC 1 cut(s) 192
GluI GCNGC 2 cut(s) 189, 686
GsaI CCCAGC 3 cut(s) 187, 540, 616
HaeIII GGCC 1 cut(s) 50
HgaI GACGC 1 cut(s) 71
HhaI GCGC 1 cut(s) 193
Hin1II CATG 4 cut(s) 106, 740, 814, 904
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HincII GTYRAC 1 cut(s) 917
HindII GTYRAC 1 cut(s) 917
HinfI GANTC 4 cut(s) 208, 590, 661, 709
HphI GGTGA 1 cut(s) 144
Hpy166II GTNNAC 1 cut(s) 917
Hpy188I TCNGA 2 cut(s) 146, 698
Hpy188III TCNNGA 7 cut(s) 218, 432, 434, 587, 668, 776, 845
Hpy8I GTNNAC 1 cut(s) 917
Hpy99I CGWCG 2 cut(s) 62, 65
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 1 cut(s) 81
HpyCH4V TGCA 7 cut(s) 304, 390, 416, 560, 638, 754, 907
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 185, 751, 904
HpyF3I CTNAG 2 cut(s) 452, 815
Hsp92II CATG 4 cut(s) 106, 740, 814, 904
HspAI GCGC 1 cut(s) 191
Ksp22I TGATCA 1 cut(s) 693
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 5 cut(s) 149, 532, 583, 693, 778
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 2 cut(s) 175, 697
LweI GCATC 4 cut(s) 431, 626, 664, 894
MaeI CTAG 1 cut(s) 360
MaeIII GTNAC 4 cut(s) 98, 170, 355, 455
MalI GATC 5 cut(s) 151, 534, 585, 695, 780
MboI GATC 5 cut(s) 149, 532, 583, 693, 778
MboII GAAGA 4 cut(s) 147, 159, 262, 902
MfeI CAATTG 1 cut(s) 299
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 2 cut(s) 322, 453
MluCI AATT 8 cut(s) 299, 375, 408, 500, 555, 639, 872, 884
MlyI GAGTC 1 cut(s) 718
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 7 cut(s) 346, 393, 434, 502, 707, 776, 932
MroXI GAANNNNTTC 1 cut(s) 42
MseI TTAA 3 cut(s) 162, 348, 503
MslI CAYNNNNRTG 1 cut(s) 669
MspA1I CMGCKG 2 cut(s) 168, 183
MspR9I CCNGG 1 cut(s) 805
MunI CAATTG 1 cut(s) 299
Mva1269I GAATGC 1 cut(s) 569
MvaI CCWGG 1 cut(s) 805
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 4 cut(s) 47, 185, 751, 904
NdeII GATC 5 cut(s) 149, 532, 583, 693, 778
NlaIII CATG 4 cut(s) 106, 740, 814, 904
NlaIV GGNNCC 1 cut(s) 534
NmuCI GTSAC 1 cut(s) 455
NspI RCATGY 1 cut(s) 814
PaeR7I CTCGAG 1 cut(s) 432
PctI GAATGC 1 cut(s) 569
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 3 cut(s) 208, 590, 661
PkrI GCNGC 2 cut(s) 190, 687
PleI GAGTC 1 cut(s) 717
PpsI GAGTC 1 cut(s) 717
PsiI TTATAA 1 cut(s) 554
Psp6I CCWGG 1 cut(s) 803
PspFI CCCAGC 3 cut(s) 183, 536, 612
PspGI CCWGG 1 cut(s) 803
PspN4I GGNNCC 1 cut(s) 534
PstI CTGCAG 1 cut(s) 392
PstNI CAGNNNCTG 2 cut(s) 17, 459
PsuI RGATCY 1 cut(s) 532
RseI CAYNNNNRTG 1 cut(s) 669
SacII CCGCGG 1 cut(s) 169
SaqAI TTAA 3 cut(s) 162, 348, 503
SatI GCNGC 2 cut(s) 189, 686
Sau3AI GATC 5 cut(s) 149, 532, 583, 693, 778
SchI GAGTC 1 cut(s) 718
ScrFI CCNGG 1 cut(s) 805
SduI GDGCHC 2 cut(s) 322, 453
SfaNI GCATC 4 cut(s) 431, 626, 664, 894
SfcI CTRYAG 1 cut(s) 388
Sfr274I CTCGAG 1 cut(s) 432
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SlaI CTCGAG 1 cut(s) 432
SmiMI CAYNNNNRTG 1 cut(s) 669
SmlI CTYRAG 2 cut(s) 432, 843
SmoI CTYRAG 2 cut(s) 432, 843
Sse9I AATT 8 cut(s) 299, 375, 408, 500, 555, 639, 872, 884
SsiI CCGC 5 cut(s) 69, 166, 168, 181, 487
SspMI CTAG 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 803
TaaI ACNGT 1 cut(s) 81
TaqI TCGA 4 cut(s) 282, 433, 712, 781
TasI AATT 8 cut(s) 299, 375, 408, 500, 555, 639, 872, 884
TfiI GAWTC 3 cut(s) 208, 590, 661
Tru1I TTAA 3 cut(s) 162, 348, 503
Tru9I TTAA 3 cut(s) 162, 348, 503
TscAI CASTG 2 cut(s) 18, 460
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 2 cut(s) 188, 685
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 5 cut(s) 119, 221, 324, 653, 897
TspRI CASTG 2 cut(s) 18, 460
XapI RAATTY 1 cut(s) 884
XceI RCATGY 1 cut(s) 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 18
XhoI CTCGAG 1 cut(s) 432
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.