RLG00000028707

Leucine carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0009548
Physical Location & Seq
Reverse (-)
26774702 .. 26780521
5820 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028707

Sequence Viewer

Length: 1026 bp
ATGGCGAAGCCAGTGGCTGATTGGCAGAGCAACAAGGAAGCAGTTCAGGCCACAAACGACGACGCTTCAGCCAGCAAACTGTCCTGCGTGAAAAAGGGTTACATGAAAGATGATTATGTCCATTTGTTTGTGAGGAGACCTGTCAGAAGATCACCCATTATTAACCGCGGTTACTTTGCCCGCTGGGCTGCGCTTCGCAAGCTTATGAATCAGTTTCTTGATACTGAAGTCGGCGAAAAGGGTGGGTCAAAGAAGCAGATACTATCGCTTGGAGCTGGCTTTGATACCACATATTTCCAGTTGCAGGATGAAGGGAGAGCACCATATTTATATGTGGAGTTGGATTTTAAGGAGGTTACTAGAAAGAAGGCAACAATTATTGAAACCTGCAGTCAAATGAGGGACAAAATTAGTGCAACAGCATCAATCTCTCGAGAGAGAGGAGAAGTGCTGAGTGACTGCTATAAGCTACTTCCTGTTGATTTGCGCGACATACATCAATTAAACGAGGTCATAGCTTTGGCTGGTATGGATCCCAGCCTGCCAACTTTTATAATTGCAGAATGCGTTTTGATATACATTGATCCAGAATCCAGTCGTGCCATAGTTGGCTGGGCATCAAAAACATTTTCAACAGCAATTTTTTTCTTATATGAGCAGATTCATCCAGATGATGCTTTTGGGCAGCAGATGATCAGAAACTTGGAGAGCCGAGGTTGTTCACTCTTGGGAATACATGATACGCCAACATTGCAGGCAAAGGAAAGACTTTTTCTTGATCGAGGATGGCAGAGAGCTGTTGCCTGGGACATGCTGAAAGTATATAGTAGTTTTGTTGAAGCTCAAGAAAGACGCAGGATTGAACGATTGGAATTGTTTGATGAATTTGAAGAATGGCACATGATGCAGGAGCACTACTGTGTGGCTTATGCAATCAATGATGCCATGAATCTTTTTGAGGATTTCGGCTTCCCTAAAGAGCAGCAGAAGCACGAGCCCATCCCCTCCTCATCAGCATCACCATGA

Protein Analysis

342

Amino Acids

39.15

Weight (kDa)

5.76

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCM PF04072 44 - 204 6.4e-19 Leucine carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
Acc36I ACCTGC 1 cut(s) 395
AccII CGCG 2 cut(s) 168, 489
AciI CCGC 3 cut(s) 166, 168, 181
AclWI GGATC 3 cut(s) 527, 540, 578
AcsI RAATTY 1 cut(s) 884
AcuI CTGAAG 2 cut(s) 51, 246
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 5 cut(s) 383, 633, 839, 863, 890
AjnI CCWGG 1 cut(s) 803
AleI CACNNNNGTG 1 cut(s) 918
AluBI AGCT 6 cut(s) 202, 275, 469, 518, 797, 842
AluI AGCT 6 cut(s) 202, 275, 469, 518, 797, 842
Alw21I GWGCWC 2 cut(s) 322, 915
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 3 cut(s) 527, 540, 578
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 432
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 3 cut(s) 188, 685, 982
ApoI RAATTY 1 cut(s) 884
Asp700I GAANNNNTTC 1 cut(s) 42
AspLEI GCGC 2 cut(s) 193, 489
AsuHPI GGTGA 2 cut(s) 144, 1011
AvaI CYCGRG 1 cut(s) 432
BaeI ACNNNNGTAYC 2 cut(s) 213, 246
BamHI GGATCC 1 cut(s) 532
BanII GRGCYC 1 cut(s) 999
BauI CACGAG 1 cut(s) 992
Bbv12I GWGCWC 2 cut(s) 322, 915
BbvI GCAGC 3 cut(s) 175, 697, 994
BccI CCATC 2 cut(s) 780, 1007
BciT130I CCWGG 1 cut(s) 805
BclI TGATCA 1 cut(s) 693
BcoDI GTCTC 1 cut(s) 130
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 388
BfuAI ACCTGC 1 cut(s) 395
BglI GCCNNNNNGGC 1 cut(s) 185
BisI GCNGC 3 cut(s) 189, 686, 983
BlsI GCNGC 3 cut(s) 190, 687, 984
Bme1390I CCNGG 1 cut(s) 805
BmeT110I CYCGRG 1 cut(s) 432
BmiI GGNNCC 1 cut(s) 534
BmrFI CCNGG 1 cut(s) 805
BmsI GCATC 5 cut(s) 431, 626, 664, 894, 931
BpuEI CTTGAG 1 cut(s) 828
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 3 cut(s) 166, 712, 804
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse1I ACTGG 3 cut(s) 11, 298, 594
Bse3DI GCAATG 1 cut(s) 749
BseBI CCWGG 1 cut(s) 805
BseDI CCNNGG 3 cut(s) 166, 712, 804
BseGI GGATG 4 cut(s) 313, 664, 791, 999
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMI GCAATG 1 cut(s) 749
BseMII CTCAG 1 cut(s) 443
BseNI ACTGG 3 cut(s) 11, 298, 594
BseRI GAGGAG 3 cut(s) 148, 456, 997
BseXI GCAGC 3 cut(s) 175, 697, 994
BseYI CCCAGC 3 cut(s) 183, 536, 612
Bsh1236I CGCG 2 cut(s) 168, 489
BshFI GGCC 1 cut(s) 50
BsiHKAI GWGCWC 2 cut(s) 322, 915
BsiHKCI CYCGRG 1 cut(s) 432
BslFI GGGAC 2 cut(s) 416, 821
BslI CCNNNNNNNGG 1 cut(s) 304
BsmAI GTCTC 1 cut(s) 130
BsmFI GGGAC 2 cut(s) 416, 821
BsmI GAATGC 1 cut(s) 569
BsnI GGCC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 432
Bsp1286I GDGCHC 3 cut(s) 322, 915, 999
Bsp143I GATC 5 cut(s) 149, 532, 583, 693, 778
BspACI CCGC 3 cut(s) 166, 168, 181
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 444
BspFNI CGCG 2 cut(s) 168, 489
BspLI GGNNCC 1 cut(s) 534
BspMAI CTGCAG 1 cut(s) 392
BspMI ACCTGC 1 cut(s) 395
BspPI GGATC 3 cut(s) 527, 540, 578
BspTNI GGTCTC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 749
BsrI ACTGG 3 cut(s) 11, 298, 594
BssECI CCNNGG 3 cut(s) 166, 712, 804
BssMI GATC 5 cut(s) 149, 532, 583, 693, 778
BssSI CACGAG 1 cut(s) 992
Bst2BI CACGAG 1 cut(s) 992
Bst2UI CCWGG 1 cut(s) 805
Bst4CI ACNGT 2 cut(s) 81, 920
BstAPI GCANNNNNTGC 1 cut(s) 904
BstC8I GCNNGC 6 cut(s) 73, 181, 200, 277, 542, 756
BstDEI CTNAG 1 cut(s) 452
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 4 cut(s) 313, 664, 791, 999
BstFNI CGCG 2 cut(s) 168, 489
BstHHI GCGC 2 cut(s) 193, 489
BstKTI GATC 5 cut(s) 152, 535, 586, 696, 781
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 5 cut(s) 149, 532, 583, 693, 778
BstMWI GCNNNNNNNGC 6 cut(s) 47, 185, 199, 751, 904, 988
BstNI CCWGG 1 cut(s) 805
BstNSI RCATGY 1 cut(s) 814
BstSCI CCNGG 1 cut(s) 803
BstSFI CTRYAG 1 cut(s) 388
BstUI CGCG 2 cut(s) 168, 489
BstV1I GCAGC 3 cut(s) 175, 697, 994
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 4 cut(s) 313, 664, 791, 999
BtsIMutI CAGTG 1 cut(s) 18
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 6 cut(s) 73, 181, 200, 277, 542, 756
CaiI CAGNNNCTG 1 cut(s) 17
CfoI GCGC 2 cut(s) 193, 489
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 2 cut(s) 71, 861
CviAII CATG 6 cut(s) 103, 737, 811, 901, 946, 1023
DdeI CTNAG 1 cut(s) 452
DpnI GATC 5 cut(s) 151, 534, 585, 695, 780
DpnII GATC 5 cut(s) 149, 532, 583, 693, 778
Eco24I GRGCYC 1 cut(s) 999
Eco31I GGTCTC 1 cut(s) 130
Eco57I CTGAAG 2 cut(s) 51, 246
Eco88I CYCGRG 1 cut(s) 432
EcoRII CCWGG 1 cut(s) 803
EcoT38I GRGCYC 1 cut(s) 999
FaeI CATG 6 cut(s) 106, 740, 814, 904, 949, 1026
FaqI GGGAC 2 cut(s) 416, 821
FatI CATG 6 cut(s) 102, 736, 810, 900, 945, 1022
FauI CCCGC 1 cut(s) 188
FbaI TGATCA 1 cut(s) 693
Fnu4HI GCNGC 3 cut(s) 189, 686, 983
FokI GGATG 4 cut(s) 320, 651, 798, 986
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 3 cut(s) 189, 686, 983
FspBI CTAG 1 cut(s) 360
GlaI GCGC 2 cut(s) 192, 488
GluI GCNGC 3 cut(s) 189, 686, 983
GsaI CCCAGC 3 cut(s) 187, 540, 616
HaeIII GGCC 1 cut(s) 50
HgaI GACGC 2 cut(s) 71, 861
HhaI GCGC 2 cut(s) 193, 489
Hin1II CATG 6 cut(s) 106, 740, 814, 904, 949, 1026
Hin6I GCGC 2 cut(s) 191, 487
HinP1I GCGC 2 cut(s) 191, 487
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 4 cut(s) 208, 590, 661, 949
HphI GGTGA 2 cut(s) 144, 1011
Hpy166II GTNNAC 1 cut(s) 722
Hpy188I TCNGA 2 cut(s) 146, 698
Hpy188III TCNNGA 7 cut(s) 218, 432, 434, 587, 668, 776, 845
Hpy8I GTNNAC 1 cut(s) 722
Hpy99I CGWCG 2 cut(s) 62, 65
HpyAV CCTTC 2 cut(s) 305, 361
HpyCH4III ACNGT 2 cut(s) 81, 920
HpyCH4V TGCA 7 cut(s) 304, 390, 416, 560, 754, 907, 932
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 185, 199, 751, 904, 988
HpyF3I CTNAG 1 cut(s) 452
Hsp92II CATG 6 cut(s) 106, 740, 814, 904, 949, 1026
HspAI GCGC 2 cut(s) 191, 487
Ksp22I TGATCA 1 cut(s) 693
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 5 cut(s) 149, 532, 583, 693, 778
LmnI GCTCC 2 cut(s) 272, 910
Lsp1109I GCAGC 3 cut(s) 175, 697, 994
LweI GCATC 5 cut(s) 431, 626, 664, 894, 931
MaeI CTAG 1 cut(s) 360
MaeIII GTNAC 4 cut(s) 98, 170, 355, 455
MalI GATC 5 cut(s) 151, 534, 585, 695, 780
MboI GATC 5 cut(s) 149, 532, 583, 693, 778
MboII GAAGA 2 cut(s) 159, 902
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 3 cut(s) 322, 915, 999
MluCI AATT 7 cut(s) 375, 408, 500, 555, 639, 872, 884
MmeI TCCRAC 1 cut(s) 321
MroXI GAANNNNTTC 1 cut(s) 42
MseI TTAA 3 cut(s) 162, 348, 503
MslI CAYNNNNRTG 3 cut(s) 669, 918, 1021
MspA1I CMGCKG 2 cut(s) 168, 183
MspR9I CCNGG 1 cut(s) 805
Mva1269I GAATGC 1 cut(s) 569
MvaI CCWGG 1 cut(s) 805
MvnI CGCG 2 cut(s) 168, 489
MwoI GCNNNNNNNGC 6 cut(s) 47, 185, 199, 751, 904, 988
NdeII GATC 5 cut(s) 149, 532, 583, 693, 778
NlaIII CATG 6 cut(s) 106, 740, 814, 904, 949, 1026
NlaIV GGNNCC 1 cut(s) 534
NmeAIII GCCGAG 1 cut(s) 737
NmuCI GTSAC 1 cut(s) 455
NspI RCATGY 1 cut(s) 814
OliI CACNNNNGTG 1 cut(s) 918
PaeR7I CTCGAG 1 cut(s) 432
PctI GAATGC 1 cut(s) 569
PdmI GAANNNNTTC 1 cut(s) 42
PfeI GAWTC 4 cut(s) 208, 590, 661, 949
PkrI GCNGC 3 cut(s) 190, 687, 984
PsiI TTATAA 1 cut(s) 554
Psp6I CCWGG 1 cut(s) 803
PspFI CCCAGC 3 cut(s) 183, 536, 612
PspGI CCWGG 1 cut(s) 803
PspN4I GGNNCC 1 cut(s) 534
PstI CTGCAG 1 cut(s) 392
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 532
RseI CAYNNNNRTG 3 cut(s) 669, 918, 1021
SacII CCGCGG 1 cut(s) 169
SaqAI TTAA 3 cut(s) 162, 348, 503
SatI GCNGC 3 cut(s) 189, 686, 983
Sau3AI GATC 5 cut(s) 149, 532, 583, 693, 778
ScrFI CCNGG 1 cut(s) 805
SduI GDGCHC 3 cut(s) 322, 915, 999
SfaNI GCATC 5 cut(s) 431, 626, 664, 894, 931
SfcI CTRYAG 1 cut(s) 388
Sfr274I CTCGAG 1 cut(s) 432
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SlaI CTCGAG 1 cut(s) 432
SmiMI CAYNNNNRTG 3 cut(s) 669, 918, 1021
SmlI CTYRAG 2 cut(s) 432, 843
SmoI CTYRAG 2 cut(s) 432, 843
Sse9I AATT 7 cut(s) 375, 408, 500, 555, 639, 872, 884
SsiI CCGC 3 cut(s) 166, 168, 181
SspMI CTAG 1 cut(s) 360
StyD4I CCNGG 1 cut(s) 803
TaaI ACNGT 2 cut(s) 81, 920
TaqI TCGA 2 cut(s) 433, 781
TasI AATT 7 cut(s) 375, 408, 500, 555, 639, 872, 884
TfiI GAWTC 4 cut(s) 208, 590, 661, 949
Tru1I TTAA 3 cut(s) 162, 348, 503
Tru9I TTAA 3 cut(s) 162, 348, 503
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 3 cut(s) 188, 685, 982
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 6 cut(s) 119, 221, 324, 653, 897, 962
TspRI CASTG 1 cut(s) 18
XapI RAATTY 1 cut(s) 884
XceI RCATGY 1 cut(s) 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 18
XhoI CTCGAG 1 cut(s) 432
XmnI GAANNNNTTC 1 cut(s) 42
XspI CTAG 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.