AT1G02860

ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007402
Physical Location & Seq
Forward (+)
635144 .. 637262
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G02860.3

Sequence Viewer

Length: 1008 bp
ATGAAGTTTTGTAAGAAGTATGAAGAGTACATGCAAGGACAGAAGGAGAAGAAGAATCTTCCTGGTGTTGGGTTTAAGAAACTCAAGAAGATTCTCAAGAGATGCAGGAGAAATCATGTTCCTTCTAGAATTTCTTTTACTGATGCAATCAACCACAATTGTTCTCGTGAATGCCCAGTTTGTGATGGGACTTTTTTCCCGGAGCTTCTCAAGGAAATGGAAGATGTTGTTGGATGGTTTAACGAGCATGCTCAGAAGCTTCTTGAGCTTCATTTAGCTTCTGGTTTTACAAAGTGTCTTACTTGGCTCAGAGGCAACAGTCGAAAAAAGGACCATCATGGTTTGATCCAAGAGGGTAAAGATTTGGTTAATTACGCTCTCATCAATGCCGTCGCCATTCGAAAAATCCTCAAGAAATATGACAAGATTCATGAGTCTAGGCAAGGACAAGCGTTTAAGACTCAGGTCCAGAAAATGCGAATAGAAATCCTTCAGTCACCGTGGCTCTGCGAGCTTATGGCGTTTCACATCAATCTGAAAGAATCTAAGAAGGAATCTGGAGCTACTATAACTTCTCCTCCTCCTCCTGTTCATGCATTGTTTGATGGTTGCGCTTTGACTTTCGACGATGGGAAGCCTTTACTTTCCTGCGAGCTCTCTGATTCCGTCAAAGTTGACATTGACTTGACTTGTTCAATATGCCTGGACACGGTGTTTGATCCAATATCTCTAACCTGCGGTCACATATATTGCTACATGTGTGCTTGCTCTGCTGCATCAGTAAACGTAGTTGATGGCTTGAAAACCGCAGAAGCAACTGAAAAATGCCCGCTTTGCCGTGAGGATGGGGTTTATAAAGGTGCTGTTCACTTGGATGAGCTCAATATTTTACTTAAGCGAAGCTGCAGAGACTATTGGGAAGAAAGGCGTAAAACAGAGAGAGCAGAAAGGTTACAACAAGCAAAGGAATATTGGGATTACCAATGCCGAAGCTTCACTGGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008219 GO:0009056 GO:0009057 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009627 GO:0009696 GO:0009697 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009987 GO:0010033 GO:0010035 GO:0010167 GO:0010337 GO:0010498 GO:0010565 GO:0010817 GO:0012501 GO:0014070 GO:0016020 GO:0016053 GO:0016567 GO:0016740 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019222 GO:0019438 GO:0019538 GO:0019752 GO:0019787 GO:0019941 GO:0030163 GO:0031323 GO:0032350 GO:0032446 GO:0032787 GO:0033554 GO:0034050 GO:0036211 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0042537 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061630 GO:0061659 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0071944 GO:0072330 GO:0080021 GO:0098542 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

335

Amino Acids

38.41

Weight (kDa)

8.6

Isoelectric Point (pI)

53.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
Acc36I ACCTGC 1 cut(s) 743
AciI CCGC 3 cut(s) 738, 807, 830
AclWI GGATC 2 cut(s) 340, 713
AcsI RAATTY 1 cut(s) 129
AcuI CTGAAG 1 cut(s) 476
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 2 cut(s) 68, 709
AflII CTTAAG 1 cut(s) 893
AflIII ACRYGT 1 cut(s) 756
AgsI TTSAA 2 cut(s) 696, 802
AjnI CCWGG 2 cut(s) 61, 702
AjuI GAANNNNNNNTTGG 2 cut(s) 213, 245
Alw21I GWGCWC 2 cut(s) 657, 882
Alw26I GTCTC 1 cut(s) 903
AlwI GGATC 2 cut(s) 340, 713
ApeKI GCWGC 2 cut(s) 773, 903
ApoI RAATTY 1 cut(s) 129
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
Asp700I GAANNNNTTC 1 cut(s) 489
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 2 cut(s) 331, 466
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 489
AsuII TTCGAA 1 cut(s) 400
AvaII GGWCC 2 cut(s) 331, 466
BanII GRGCYC 2 cut(s) 657, 882
BauI CACGAG 1 cut(s) 165
Bbv12I GWGCWC 2 cut(s) 657, 882
BbvI GCAGC 2 cut(s) 760, 890
BccI CCATC 7 cut(s) 179, 228, 342, 599, 623, 788, 839
BceAI ACGGC 2 cut(s) 374, 822
BciT130I CCWGG 2 cut(s) 63, 704
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 903
BfaI CTAG 2 cut(s) 126, 438
BfmI CTRYAG 1 cut(s) 904
BfrI CTTAAG 1 cut(s) 893
BfuAI ACCTGC 1 cut(s) 743
BisI GCNGC 2 cut(s) 774, 904
BlsI GCNGC 2 cut(s) 775, 905
Bme1390I CCNGG 3 cut(s) 63, 200, 704
Bme18I GGWCC 2 cut(s) 331, 466
BmgT120I GGNCC 2 cut(s) 331, 466
BmrFI CCNGG 3 cut(s) 63, 200, 704
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 3 cut(s) 92, 133, 785
BmuI ACTGGG 1 cut(s) 170
BoxI GACNNNNGTC 1 cut(s) 464
BpmI CTGGAG 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 400
BpuEI CTTGAG 5 cut(s) 68, 80, 194, 284, 395
BpuMI CCSGG 1 cut(s) 200
BsaJI CCNNGG 1 cut(s) 500
BsaXI ACNNNNNCTCC 2 cut(s) 562, 592
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 709
Bse1I ACTGG 2 cut(s) 176, 1003
BseBI CCWGG 2 cut(s) 63, 704
BseDI CCNNGG 1 cut(s) 500
BseGI GGATG 3 cut(s) 239, 850, 880
BseLI CCNNNNNNNGG 2 cut(s) 68, 709
BseMII CTCAG 3 cut(s) 266, 322, 476
BseNI ACTGG 2 cut(s) 176, 1003
BseRI GAGGAG 3 cut(s) 567, 570, 573
BseXI GCAGC 2 cut(s) 760, 890
BsiHKAI GWGCWC 2 cut(s) 657, 882
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 2 cut(s) 68, 709
BsmAI GTCTC 1 cut(s) 903
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 176
Bsp119I TTCGAA 1 cut(s) 400
Bsp1286I GDGCHC 2 cut(s) 657, 882
Bsp143I GATC 2 cut(s) 345, 718
BspACI CCGC 3 cut(s) 738, 807, 830
BspCNI CTCAG 3 cut(s) 265, 321, 475
BspHI TCATGA 1 cut(s) 430
BspMAI CTGCAG 1 cut(s) 908
BspMI ACCTGC 1 cut(s) 743
BspPI GGATC 2 cut(s) 340, 713
BspT104I TTCGAA 1 cut(s) 400
BspTI CTTAAG 1 cut(s) 893
BsrI ACTGG 2 cut(s) 176, 1003
BssECI CCNNGG 1 cut(s) 500
BssMI GATC 2 cut(s) 345, 718
BssSI CACGAG 1 cut(s) 165
Bst2BI CACGAG 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 63, 704
Bst4CI ACNGT 3 cut(s) 320, 501, 712
Bst6I CTCTTC 1 cut(s) 18
BstAFI CTTAAG 1 cut(s) 893
BstBI TTCGAA 1 cut(s) 400
BstC8I GCNNGC 5 cut(s) 249, 512, 653, 766, 830
BstDEI CTNAG 4 cut(s) 252, 308, 462, 546
BstDSI CCRYGG 1 cut(s) 500
BstF5I GGATG 3 cut(s) 239, 850, 880
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 2 cut(s) 348, 721
BstMAI GTCTC 1 cut(s) 903
BstMBI GATC 2 cut(s) 345, 718
BstMWI GCNNNNNNNGC 4 cut(s) 265, 511, 770, 834
BstNI CCWGG 2 cut(s) 63, 704
BstNSI RCATGY 3 cut(s) 34, 251, 760
BstPAI GACNNNNGTC 1 cut(s) 464
BstSCI CCNGG 3 cut(s) 61, 198, 702
BstSFI CTRYAG 1 cut(s) 904
BstV1I GCAGC 2 cut(s) 760, 890
BtgI CCRYGG 1 cut(s) 500
BtsCI GGATG 3 cut(s) 239, 850, 880
BtsIMutI CAGTG 1 cut(s) 996
BveI ACCTGC 1 cut(s) 743
Cac8I GCNNGC 5 cut(s) 249, 512, 653, 766, 830
CciI TCATGA 1 cut(s) 430
CfoI GCGC 1 cut(s) 614
Cfr13I GGNCC 2 cut(s) 331, 466
Csp6I GTAC 1 cut(s) 28
CviAII CATG 7 cut(s) 31, 116, 248, 338, 431, 593, 757
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 4 cut(s) 252, 308, 462, 546
DpnI GATC 2 cut(s) 347, 720
DpnII GATC 2 cut(s) 345, 718
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Ecl136II GAGCTC 2 cut(s) 655, 880
Eco24I GRGCYC 2 cut(s) 657, 882
Eco47I GGWCC 2 cut(s) 331, 466
Eco53kI GAGCTC 2 cut(s) 655, 880
Eco57I CTGAAG 1 cut(s) 476
EcoICRI GAGCTC 2 cut(s) 655, 880
EcoRII CCWGG 2 cut(s) 61, 702
EcoT22I ATGCAT 1 cut(s) 598
EcoT38I GRGCYC 2 cut(s) 657, 882
FaeI CATG 7 cut(s) 34, 119, 251, 341, 434, 596, 760
FaqI GGGAC 1 cut(s) 202
FatI CATG 7 cut(s) 30, 115, 247, 337, 430, 592, 756
FauI CCCGC 1 cut(s) 837
Fnu4HI GCNGC 2 cut(s) 774, 904
FokI GGATG 3 cut(s) 246, 857, 887
FriOI GRGCYC 2 cut(s) 657, 882
Fsp4HI GCNGC 2 cut(s) 774, 904
FspBI CTAG 2 cut(s) 126, 438
GlaI GCGC 1 cut(s) 613
GluI GCNGC 2 cut(s) 774, 904
GsuI CTGGAG 1 cut(s) 579
HapII CCGG 1 cut(s) 200
HhaI GCGC 1 cut(s) 614
Hin1II CATG 7 cut(s) 34, 119, 251, 341, 434, 596, 760
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HindIII AAGCTT 2 cut(s) 257, 991
HinfI GANTC 8 cut(s) 55, 91, 427, 434, 460, 542, 554, 662
HpaII CCGG 1 cut(s) 200
HphI GGTGA 1 cut(s) 489
Hpy166II GTNNAC 3 cut(s) 676, 784, 868
Hpy188I TCNGA 4 cut(s) 255, 311, 537, 661
Hpy188III TCNNGA 9 cut(s) 85, 97, 126, 167, 263, 412, 431, 469, 558
Hpy8I GTNNAC 3 cut(s) 676, 784, 868
Hpy99I CGWCG 2 cut(s) 395, 629
HpyAV CCTTC 4 cut(s) 37, 132, 500, 544
HpyCH4III ACNGT 3 cut(s) 320, 501, 712
HpyCH4IV ACGT 1 cut(s) 786
HpyCH4V TGCA 6 cut(s) 34, 105, 146, 596, 776, 906
HpyF10VI GCNNNNNNNGC 4 cut(s) 265, 511, 770, 834
HpyF3I CTNAG 4 cut(s) 252, 308, 462, 546
HpySE526I ACGT 1 cut(s) 786
Hsp92II CATG 7 cut(s) 34, 119, 251, 341, 434, 596, 760
HspAI GCGC 1 cut(s) 612
Kzo9I GATC 2 cut(s) 345, 718
LmnI GCTCC 2 cut(s) 202, 560
Lsp1109I GCAGC 2 cut(s) 760, 890
LweI GCATC 3 cut(s) 92, 133, 785
MaeI CTAG 2 cut(s) 126, 438
MaeII ACGT 1 cut(s) 786
MaeIII GTNAC 3 cut(s) 495, 740, 951
MalI GATC 2 cut(s) 347, 720
MboI GATC 2 cut(s) 345, 718
MboII GAAGA 7 cut(s) 35, 50, 61, 64, 100, 233, 932
MfeI CAATTG 1 cut(s) 157
MhlI GDGCHC 2 cut(s) 657, 882
MluCI AATT 3 cut(s) 129, 157, 370
MlyI GAGTC 2 cut(s) 443, 454
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 7 cut(s) 305, 346, 419, 588, 591, 594, 835
Mph1103I ATGCAT 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 489
MseI TTAA 5 cut(s) 75, 240, 369, 456, 894
MslI CAYNNNNRTG 1 cut(s) 873
MspCI CTTAAG 1 cut(s) 893
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 3 cut(s) 63, 200, 704
MunI CAATTG 1 cut(s) 157
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 2 cut(s) 63, 704
MwoI GCNNNNNNNGC 4 cut(s) 265, 511, 770, 834
NciI CCSGG 1 cut(s) 200
NdeII GATC 2 cut(s) 345, 718
NlaIII CATG 7 cut(s) 34, 119, 251, 341, 434, 596, 760
NmuCI GTSAC 2 cut(s) 495, 740
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 3 cut(s) 34, 251, 760
NspV TTCGAA 1 cut(s) 400
PaeI GCATGC 1 cut(s) 251
PagI TCATGA 1 cut(s) 430
PciI ACATGT 1 cut(s) 756
PctI GAATGC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 6 cut(s) 55, 91, 427, 542, 554, 662
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 2 cut(s) 775, 905
PleI GAGTC 2 cut(s) 442, 454
PpsI GAGTC 2 cut(s) 442, 454
PscI ACATGT 1 cut(s) 756
PshAI GACNNNNGTC 1 cut(s) 464
PsiI TTATAA 1 cut(s) 855
Psp124BI GAGCTC 2 cut(s) 657, 882
Psp6I CCWGG 2 cut(s) 61, 702
PspGI CCWGG 2 cut(s) 61, 702
PspPI GGNCC 2 cut(s) 331, 466
PstI CTGCAG 1 cut(s) 908
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 1 cut(s) 873
SacI GAGCTC 2 cut(s) 657, 882
SaqAI TTAA 5 cut(s) 75, 240, 369, 456, 894
SatI GCNGC 2 cut(s) 774, 904
Sau3AI GATC 2 cut(s) 345, 718
Sau96I GGNCC 2 cut(s) 331, 466
SchI GAGTC 2 cut(s) 443, 454
ScrFI CCNGG 3 cut(s) 63, 200, 704
SduI GDGCHC 2 cut(s) 657, 882
SfaNI GCATC 3 cut(s) 92, 133, 785
SfcI CTRYAG 1 cut(s) 904
SfuI TTCGAA 1 cut(s) 400
SinI GGWCC 2 cut(s) 331, 466
SmiMI CAYNNNNRTG 1 cut(s) 873
SmlI CTYRAG 6 cut(s) 83, 95, 209, 263, 410, 893
SmoI CTYRAG 6 cut(s) 83, 95, 209, 263, 410, 893
SphI GCATGC 1 cut(s) 251
Sse9I AATT 3 cut(s) 129, 157, 370
SsiI CCGC 3 cut(s) 738, 807, 830
SspI AATATT 2 cut(s) 886, 971
SspMI CTAG 2 cut(s) 126, 438
SstI GAGCTC 2 cut(s) 657, 882
StyD4I CCNGG 3 cut(s) 61, 198, 702
TaaI ACNGT 3 cut(s) 320, 501, 712
TaiI ACGT 1 cut(s) 789
TaqI TCGA 3 cut(s) 322, 400, 624
TasI AATT 3 cut(s) 129, 157, 370
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 6 cut(s) 55, 91, 427, 542, 554, 662
Tru1I TTAA 5 cut(s) 75, 240, 369, 456, 894
Tru9I TTAA 5 cut(s) 75, 240, 369, 456, 894
TscAI CASTG 1 cut(s) 1003
TseFI GTSAC 2 cut(s) 495, 740
TseI GCWGC 2 cut(s) 773, 903
Tsp45I GTSAC 2 cut(s) 495, 740
TspDTI ATGAA 5 cut(s) 17, 36, 260, 419, 581
TspGWI ACGGA 1 cut(s) 655
TspRI CASTG 1 cut(s) 1003
Vha464I CTTAAG 1 cut(s) 893
VpaK11BI GGWCC 2 cut(s) 331, 466
XapI RAATTY 1 cut(s) 129
XbaI TCTAGA 1 cut(s) 125
XceI RCATGY 3 cut(s) 34, 251, 760
XmnI GAANNNNTTC 1 cut(s) 489
XspI CTAG 2 cut(s) 126, 438
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.