Rh6DG352500

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0007402
Physical Location & Seq
Reverse (-)
55208562 .. 55211712
3151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG352500.1

Sequence Viewer

Length: 735 bp
ATGAAGTTCTGCAAGAAATACCAGGAGTACATGCAAGGCCAAGGGAAGAAGCTACCTGGGGTTGGCTTCAAGAAGCTTAAGAAGATCTTGAAGAAATGCCGCAGAGATTTTCAGTCCGGAAATGACATTAATAATCATGCCGTACATGATCCCAGAACATGCCCTGACCATTGCCCAGTGTGTGATGGGTCGTTTTTCCCTTCACTGCTCAATGAAATGTCTGCAGTGGTAGGCTTTTTTAACCAGAAGGCACAGAAATTGCTCGAGTACCATTTGGCTTCTGGGTTTCGCAAGTACATGCTTGTGTTCAAAGGCAAGCCTCGAGGAAACCATGTTGCCTTAATCCAAGAAGGCAAGGACCTTGTCACTTATGCTCTCATTAACGCCATTGCGATTCGAAAAATACTCAAGAAATATGACAAGATTCATTACTCAAAGCAAGGGCAGGCCTTTAAGTCACAAGCGCAAAGTATGCACATTGGAATTCTTCAAAGTCCTTGGCTTTGTGAGCTTATGGCTTTCCACATCAATTTAAGGCAATCGAAGATCAAGTCGGGGAAGAGACCGGCGTTGTTTGAGGGATGCTCTCTTACAATGAATGATGAGAAGCCGTCTCTCACCTGTGAGCTCTTTGATTCTGTCAAAGTTGATATTGACTTGACATGTTCTATATGCCTGGTGAGTATCCAAGGTTTCATTTGTTTTAAGAATGTCTCGCATATCATGCATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008219 GO:0009056 GO:0009057 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009627 GO:0009696 GO:0009697 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009987 GO:0010033 GO:0010035 GO:0010167 GO:0010337 GO:0010498 GO:0010565 GO:0010817 GO:0012501 GO:0014070 GO:0016020 GO:0016053 GO:0016567 GO:0016740 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019222 GO:0019438 GO:0019538 GO:0019752 GO:0019787 GO:0019941 GO:0030163 GO:0031323 GO:0032350 GO:0032446 GO:0032787 GO:0033554 GO:0034050 GO:0036211 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0042537 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061630 GO:0061659 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0071944 GO:0072330 GO:0080021 GO:0098542 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

244

Amino Acids

27.74

Weight (kDa)

9.5

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 321
AccIII TCCGGA 1 cut(s) 116
AciI CCGC 1 cut(s) 100
AclWI GGATC 1 cut(s) 143
AcsI RAATTY 1 cut(s) 483
AfaI GTAC 4 cut(s) 29, 144, 269, 296
AfiI CCNNNNNNNGG 1 cut(s) 62
AflII CTTAAG 1 cut(s) 77
AflIII ACRYGT 1 cut(s) 662
AgsI TTSAA 4 cut(s) 70, 91, 310, 491
AjnI CCWGG 3 cut(s) 21, 55, 675
AluBI AGCT 4 cut(s) 52, 76, 511, 628
AluI AGCT 4 cut(s) 52, 76, 511, 628
Alw21I GWGCWC 1 cut(s) 630
Alw26I GTCTC 3 cut(s) 556, 618, 718
AlwI GGATC 1 cut(s) 143
Ama87I CYCGRG 2 cut(s) 263, 321
Aor13HI TCCGGA 1 cut(s) 116
AoxI GGCC 2 cut(s) 37, 447
ApoI RAATTY 1 cut(s) 483
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
AseI ATTAAT 1 cut(s) 129
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 358
AsuHPI GGTGA 2 cut(s) 610, 691
AsuII TTCGAA 1 cut(s) 397
AvaI CYCGRG 2 cut(s) 263, 321
AvaII GGWCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 630
BccI CCATC 1 cut(s) 179
BceAI ACGGC 2 cut(s) 125, 595
BciT130I CCWGG 3 cut(s) 23, 57, 677
BciVI GTATCC 1 cut(s) 695
BcoDI GTCTC 3 cut(s) 556, 618, 718
BfmI CTRYAG 1 cut(s) 222
BfrI CTTAAG 1 cut(s) 77
BfuI GTATCC 1 cut(s) 695
BglII AGATCT 1 cut(s) 84
BisI GCNGC 1 cut(s) 100
BlsI GCNGC 1 cut(s) 101
Bme1390I CCNGG 3 cut(s) 23, 57, 677
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 2 cut(s) 263, 321
BmgT120I GGNCC 1 cut(s) 358
BmrFI CCNGG 3 cut(s) 23, 57, 677
BmrI ACTGGG 1 cut(s) 170
BmsI GCATC 1 cut(s) 572
BmuI ACTGGG 1 cut(s) 170
BplI GAGNNNNNCTC 2 cut(s) 569, 601
Bpu14I TTCGAA 1 cut(s) 397
BpuEI CTTGAG 1 cut(s) 392
BsaI GGTCTC 1 cut(s) 556
BsaJI CCNNGG 4 cut(s) 40, 56, 497, 688
BsaWI WCCGGW 1 cut(s) 116
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse118I RCCGGY 1 cut(s) 565
Bse1I ACTGG 1 cut(s) 176
Bse3DI GCAATG 2 cut(s) 169, 387
BseAI TCCGGA 1 cut(s) 116
BseBI CCWGG 3 cut(s) 23, 57, 677
BseDI CCNNGG 4 cut(s) 40, 56, 497, 688
BseGI GGATG 1 cut(s) 587
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMI GCAATG 2 cut(s) 169, 387
BseNI ACTGG 1 cut(s) 176
BshFI GGCC 2 cut(s) 39, 449
BsiHKAI GWGCWC 1 cut(s) 630
BsiHKCI CYCGRG 2 cut(s) 263, 321
BsiSI CCGG 2 cut(s) 117, 566
BslI CCNNNNNNNGG 1 cut(s) 62
BsmAI GTCTC 3 cut(s) 556, 618, 718
BsmBI CGTCTC 1 cut(s) 618
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 2 cut(s) 39, 449
Bso31I GGTCTC 1 cut(s) 556
BsoBI CYCGRG 2 cut(s) 263, 321
Bsp119I TTCGAA 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 630
Bsp13I TCCGGA 1 cut(s) 116
Bsp143I GATC 3 cut(s) 84, 148, 546
BspACI CCGC 1 cut(s) 100
BspANI GGCC 2 cut(s) 39, 449
BspEI TCCGGA 1 cut(s) 116
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 1 cut(s) 143
BspT104I TTCGAA 1 cut(s) 397
BspTI CTTAAG 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 556
BsrDI GCAATG 2 cut(s) 169, 387
BsrFI RCCGGY 1 cut(s) 565
BsrI ACTGG 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 565
BssECI CCNNGG 4 cut(s) 40, 56, 497, 688
BssMI GATC 3 cut(s) 84, 148, 546
BssT1I CCWWGG 3 cut(s) 40, 497, 688
Bst2UI CCWGG 3 cut(s) 23, 57, 677
Bst6I CTCTTC 1 cut(s) 554
BstAFI CTTAAG 1 cut(s) 77
BstAPI GCANNNNNTGC 2 cut(s) 472, 724
BstBI TTCGAA 1 cut(s) 397
BstC8I GCNNGC 2 cut(s) 317, 447
BstF5I GGATG 1 cut(s) 587
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 3 cut(s) 87, 151, 549
BstMAI GTCTC 3 cut(s) 556, 618, 718
BstMBI GATC 3 cut(s) 84, 148, 546
BstMWI GCNNNNNNNGC 3 cut(s) 472, 508, 724
BstNI CCWGG 3 cut(s) 23, 57, 677
BstNSI RCATGY 4 cut(s) 34, 162, 301, 666
BstSCI CCNGG 3 cut(s) 21, 55, 675
BstSFI CTRYAG 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuI GTATCC 1 cut(s) 695
BsuRI GGCC 2 cut(s) 39, 449
BtsCI GGATG 1 cut(s) 587
BtsI GCAGTG 2 cut(s) 203, 231
BtsIMutI CAGTG 3 cut(s) 183, 203, 231
Cac8I GCNNGC 2 cut(s) 317, 447
CfoI GCGC 1 cut(s) 466
Cfr10I RCCGGY 1 cut(s) 565
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 4 cut(s) 28, 143, 268, 295
CviAII CATG 8 cut(s) 31, 137, 146, 159, 298, 332, 663, 724
CviQI GTAC 4 cut(s) 28, 143, 268, 295
DpnI GATC 3 cut(s) 86, 150, 548
DpnII GATC 3 cut(s) 84, 148, 546
Eam1104I CTCTTC 1 cut(s) 554
EarI CTCTTC 1 cut(s) 554
Ecl136II GAGCTC 1 cut(s) 628
Eco130I CCWWGG 3 cut(s) 40, 497, 688
Eco147I AGGCCT 1 cut(s) 449
Eco24I GRGCYC 1 cut(s) 630
Eco31I GGTCTC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 628
Eco88I CYCGRG 2 cut(s) 263, 321
EcoICRI GAGCTC 1 cut(s) 628
EcoO109I RGGNCCY 1 cut(s) 358
EcoRI GAATTC 1 cut(s) 483
EcoRII CCWGG 3 cut(s) 21, 55, 675
EcoT14I CCWWGG 3 cut(s) 40, 497, 688
EcoT22I ATGCAT 1 cut(s) 729
EcoT38I GRGCYC 1 cut(s) 630
ErhI CCWWGG 3 cut(s) 40, 497, 688
Esp3I CGTCTC 1 cut(s) 618
FaeI CATG 8 cut(s) 34, 140, 149, 162, 301, 335, 666, 727
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FatI CATG 8 cut(s) 30, 136, 145, 158, 297, 331, 662, 723
Fnu4HI GCNGC 1 cut(s) 100
FokI GGATG 1 cut(s) 594
FriOI GRGCYC 1 cut(s) 630
Fsp4HI GCNGC 1 cut(s) 100
GlaI GCGC 1 cut(s) 465
GluI GCNGC 1 cut(s) 100
HaeIII GGCC 2 cut(s) 39, 449
HapII CCGG 2 cut(s) 117, 566
HhaI GCGC 1 cut(s) 466
Hin1II CATG 8 cut(s) 34, 140, 149, 162, 301, 335, 666, 727
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 3 cut(s) 394, 424, 635
HpaII CCGG 2 cut(s) 117, 566
HphI GGTGA 2 cut(s) 610, 691
Hpy188III TCNNGA 5 cut(s) 70, 88, 117, 409, 732
HpyAV CCTTC 3 cut(s) 210, 241, 344
HpyCH4V TGCA 5 cut(s) 12, 34, 224, 475, 727
HpyF10VI GCNNNNNNNGC 3 cut(s) 472, 508, 724
Hsp92II CATG 8 cut(s) 34, 140, 149, 162, 301, 335, 666, 727
HspAI GCGC 1 cut(s) 464
Kpn2I TCCGGA 1 cut(s) 116
Kzo9I GATC 3 cut(s) 84, 148, 546
LweI GCATC 1 cut(s) 572
MaeIII GTNAC 2 cut(s) 364, 456
MalI GATC 3 cut(s) 86, 150, 548
MboI GATC 3 cut(s) 84, 148, 546
MboII GAAGA 6 cut(s) 58, 94, 103, 479, 556, 571
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 630
MluCI AATT 3 cut(s) 257, 483, 529
MnlI CCTC 3 cut(s) 317, 330, 571
Mph1103I ATGCAT 1 cut(s) 729
MroI TCCGGA 1 cut(s) 116
MseI TTAA 8 cut(s) 78, 129, 240, 341, 381, 453, 533, 705
MslI CAYNNNNRTG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 2 cut(s) 117, 566
MspR9I CCNGG 3 cut(s) 23, 57, 677
Mva1269I GAATGC 1 cut(s) 727
MvaI CCWGG 3 cut(s) 23, 57, 677
MwoI GCNNNNNNNGC 3 cut(s) 472, 508, 724
NdeII GATC 3 cut(s) 84, 148, 546
NlaIII CATG 8 cut(s) 34, 140, 149, 162, 301, 335, 666, 727
NmuCI GTSAC 2 cut(s) 364, 456
NsiI ATGCAT 1 cut(s) 729
NspI RCATGY 4 cut(s) 34, 162, 301, 666
NspV TTCGAA 1 cut(s) 397
PaeR7I CTCGAG 2 cut(s) 263, 321
PceI AGGCCT 1 cut(s) 449
PciI ACATGT 1 cut(s) 662
PctI GAATGC 1 cut(s) 727
PfeI GAWTC 3 cut(s) 394, 424, 635
PflFI GACNNNGTC 1 cut(s) 362
PkrI GCNGC 1 cut(s) 101
PpuMI RGGWCCY 1 cut(s) 358
PscI ACATGT 1 cut(s) 662
PshBI ATTAAT 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 630
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 3 cut(s) 21, 55, 675
PspGI CCWGG 3 cut(s) 21, 55, 675
PspPI GGNCC 1 cut(s) 358
PspPPI RGGWCCY 1 cut(s) 358
PspXI VCTCGAGB 2 cut(s) 263, 321
PstI CTGCAG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 84
PsyI GACNNNGTC 1 cut(s) 362
RsaI GTAC 4 cut(s) 29, 144, 269, 296
RsaNI GTAC 4 cut(s) 28, 143, 268, 295
RseI CAYNNNNRTG 1 cut(s) 302
SacI GAGCTC 1 cut(s) 630
SaqAI TTAA 8 cut(s) 78, 129, 240, 341, 381, 453, 533, 705
SatI GCNGC 1 cut(s) 100
Sau3AI GATC 3 cut(s) 84, 148, 546
Sau96I GGNCC 1 cut(s) 358
ScrFI CCNGG 3 cut(s) 23, 57, 677
SduI GDGCHC 1 cut(s) 630
SetI ASST 8 cut(s) 54, 58, 78, 363, 513, 623, 630, 694
SfaNI GCATC 1 cut(s) 572
SfcI CTRYAG 1 cut(s) 222
Sfr274I CTCGAG 2 cut(s) 263, 321
SfuI TTCGAA 1 cut(s) 397
SinI GGWCC 1 cut(s) 358
SlaI CTCGAG 2 cut(s) 263, 321
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 4 cut(s) 77, 263, 321, 407
SmoI CTYRAG 4 cut(s) 77, 263, 321, 407
Sse9I AATT 3 cut(s) 257, 483, 529
SseBI AGGCCT 1 cut(s) 449
SsiI CCGC 1 cut(s) 100
SstI GAGCTC 1 cut(s) 630
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 3 cut(s) 21, 55, 675
StyI CCWWGG 3 cut(s) 40, 497, 688
TaqI TCGA 4 cut(s) 264, 322, 397, 542
TasI AATT 3 cut(s) 257, 483, 529
TatI WGTACW 2 cut(s) 27, 294
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 3 cut(s) 394, 424, 635
Tru1I TTAA 8 cut(s) 78, 129, 240, 341, 381, 453, 533, 705
Tru9I TTAA 8 cut(s) 78, 129, 240, 341, 381, 453, 533, 705
TscAI CASTG 3 cut(s) 183, 210, 231
TseFI GTSAC 2 cut(s) 364, 456
Tsp45I GTSAC 2 cut(s) 364, 456
TspDTI ATGAA 5 cut(s) 17, 228, 416, 611, 685
TspRI CASTG 3 cut(s) 183, 210, 231
Tth111I GACNNNGTC 1 cut(s) 362
Vha464I CTTAAG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 358
VspI ATTAAT 1 cut(s) 129
XapI RAATTY 1 cut(s) 483
XceI RCATGY 4 cut(s) 34, 162, 301, 666
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XhoI CTCGAG 2 cut(s) 263, 321
Zsp2I ATGCAT 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.