Prupe.8G260000_v2.0.a1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0007402
Physical Location & Seq
Reverse (-)
22011037 .. 22014354
3318 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G260000.1

Sequence Viewer

Length: 960 bp
ATGAAGTTCTGCAAGAAATACCAGGAGTACATGCAAGGCCAGGAGAAGAAGCTACCTGGGGTTGGCTTCAAGAAGCTGAAGAAGATCTTGAAGAAATGCCGCAGGGAGGACACTCATGAGGTCCATGACGTTCGAACATGCCCTGACCATTGCCCAGTGTGTGATGGGAGCTTTTTCCCTGCCCTTCTCAAGGAGATGTCTGCAGTGGTAGGCTTCTTTAATAAGCGTGCCCAGAAATTGCTTGACCTGCATCTGGCTTCTGGCTTTCGTAAGTACTTCTTTTGGTTCAAAGGCAAACTTCGAGGGAACCATGTTGGCTTAATTGAAGAAGGCAAGGACCTTGTTACTTATGCACTGATCAACGCCATTGCCATTCGAAAAATACTAAAGAAATATGATAAGATTCATTACTCAACGCAAGGACAGGCCTTTAAATCGCAAGCTCAAAGTATGCACATTGGAATCCTTCAGAGTCCATGGCTTTGTGAGCTTATGGCTTTTCACATCAATTTAAGGGAAACCAAGACCAAGTCAAGGAAGACACCAGCCTTGTTTGAGGGATGCTCCCTCAAAATCAATGATGGGAAACCATCTCTCGCCTGCGAGCTCTTTGATTCTGTCAAGCTTGATGTTGACTTGACATGTTCTATATGCTTGGACACAGTTTTTGATCCAGTTGCTCTCACATGTGGTCATATATTCTGCTTTATGTGCGCTTGCTCGGCTGCATCAGTGACTATTGTCGATGGATTAAAGGCTGCAGAGCCTAAAGAAAAATGCCCTCTATGCCGAGAGACAAGAGTGTATGAGGGTGCTGTACACTTAGAAGAGCTTAGCATGTTACTTAGCAGAAGCTGCCATGAATACTGGGAGCAGAGGCTCCAGACAGAAAGGATAGAGAGGATTCGGCAAGCAAAGCAGCACTGGGAATCCCAGTGTCGGGCGTTCATGGGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008219 GO:0009056 GO:0009057 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009627 GO:0009696 GO:0009697 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009987 GO:0010033 GO:0010035 GO:0010167 GO:0010337 GO:0010498 GO:0010565 GO:0010817 GO:0012501 GO:0014070 GO:0016020 GO:0016053 GO:0016567 GO:0016740 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019222 GO:0019438 GO:0019538 GO:0019752 GO:0019787 GO:0019941 GO:0030163 GO:0031323 GO:0032350 GO:0032446 GO:0032787 GO:0033554 GO:0034050 GO:0036211 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0042537 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061630 GO:0061659 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0071944 GO:0072330 GO:0080021 GO:0098542 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

320

Amino Acids

36.57

Weight (kDa)

9.02

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 255
AciI CCGC 1 cut(s) 100
AclWI GGATC 1 cut(s) 665
AcuI CTGAAG 2 cut(s) 98, 452
AfaI GTAC 3 cut(s) 29, 275, 819
AfiI CCNNNNNNNGG 7 cut(s) 62, 106, 190, 253, 534, 939, 940
AflIII ACRYGT 2 cut(s) 641, 686
AgsI TTSAA 4 cut(s) 70, 91, 289, 326
AjnI CCWGG 3 cut(s) 21, 39, 55
AluBI AGCT 9 cut(s) 52, 76, 171, 443, 490, 607, 625, 832, 855
AluI AGCT 9 cut(s) 52, 76, 171, 443, 490, 607, 625, 832, 855
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 1 cut(s) 788
AlwI GGATC 1 cut(s) 665
AlwNI CAGNNNCTG 1 cut(s) 855
AoxI GGCC 2 cut(s) 37, 426
ApeKI GCWGC 4 cut(s) 725, 758, 855, 919
ArsI GACNNNNNNTTYG 2 cut(s) 650, 682
AspLEI GCGC 1 cut(s) 716
AspS9I GGNCC 2 cut(s) 121, 337
AsuII TTCGAA 2 cut(s) 133, 376
AvaII GGWCC 2 cut(s) 121, 337
BaeGI GKGCMC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 609
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 4 cut(s) 712, 745, 842, 931
BccI CCATC 4 cut(s) 158, 575, 598, 740
BciT130I CCWGG 3 cut(s) 23, 41, 57
BclI TGATCA 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 788
BfmI CTRYAG 2 cut(s) 201, 759
BfuAI ACCTGC 1 cut(s) 255
BglII AGATCT 1 cut(s) 84
BisI GCNGC 5 cut(s) 100, 726, 759, 856, 920
BlpI GCTNAGC 1 cut(s) 833
BlsI GCNGC 5 cut(s) 101, 727, 760, 857, 921
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 3 cut(s) 23, 41, 57
Bme18I GGWCC 2 cut(s) 121, 337
BmgT120I GGNCC 2 cut(s) 121, 337
BmiI GGNNCC 2 cut(s) 308, 881
BmrFI CCNGG 3 cut(s) 23, 41, 57
BmrI ACTGGG 4 cut(s) 149, 877, 928, 934
BmsI GCATC 3 cut(s) 259, 551, 737
BmuI ACTGGG 4 cut(s) 149, 877, 928, 934
BoxI GACNNNNGTC 1 cut(s) 740
BpiI GAAGAC 1 cut(s) 545
BplI GAGNNNNNCTC 2 cut(s) 548, 580
BpmI CTGGAG 1 cut(s) 866
Bpu1102I GCTNAGC 1 cut(s) 833
Bpu14I TTCGAA 2 cut(s) 133, 376
BpuEI CTTGAG 1 cut(s) 173
BsaJI CCNNGG 2 cut(s) 56, 476
Bsc4I CCNNNNNNNGG 7 cut(s) 62, 106, 190, 253, 534, 939, 940
Bse1I ACTGG 5 cut(s) 155, 674, 872, 929, 934
Bse3DI GCAATG 2 cut(s) 148, 366
BseBI CCWGG 3 cut(s) 23, 41, 57
BseDI CCNNGG 2 cut(s) 56, 476
BseGI GGATG 1 cut(s) 566
BseLI CCNNNNNNNGG 7 cut(s) 62, 106, 190, 253, 534, 939, 940
BseMI GCAATG 2 cut(s) 148, 366
BseNI ACTGG 5 cut(s) 155, 674, 872, 929, 934
BseSI GKGCMC 1 cut(s) 232
BseXI GCAGC 4 cut(s) 712, 745, 842, 931
BshFI GGCC 2 cut(s) 39, 428
BsiHKAI GWGCWC 1 cut(s) 609
BslI CCNNNNNNNGG 7 cut(s) 62, 106, 190, 253, 534, 939, 940
BsmAI GTCTC 1 cut(s) 788
BsnI GGCC 2 cut(s) 39, 428
Bsp119I TTCGAA 2 cut(s) 133, 376
Bsp1286I GDGCHC 2 cut(s) 232, 609
Bsp1407I TGTACA 1 cut(s) 817
Bsp143I GATC 3 cut(s) 84, 357, 670
Bsp1720I GCTNAGC 1 cut(s) 833
Bsp19I CCATGG 1 cut(s) 476
BspACI CCGC 1 cut(s) 100
BspANI GGCC 2 cut(s) 39, 428
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 308, 881
BspMAI CTGCAG 2 cut(s) 205, 763
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 1 cut(s) 665
BspQI GCTCTTC 1 cut(s) 822
BspT104I TTCGAA 2 cut(s) 133, 376
BsrDI GCAATG 2 cut(s) 148, 366
BsrGI TGTACA 1 cut(s) 817
BsrI ACTGG 5 cut(s) 155, 674, 872, 929, 934
BssECI CCNNGG 2 cut(s) 56, 476
BssMI GATC 3 cut(s) 84, 357, 670
BssT1I CCWWGG 1 cut(s) 476
Bst2UI CCWGG 3 cut(s) 23, 41, 57
Bst4CI ACNGT 1 cut(s) 664
Bst6I CTCTTC 1 cut(s) 822
BstAPI GCANNNNNTGC 1 cut(s) 855
BstAUI TGTACA 1 cut(s) 817
BstBI TTCGAA 2 cut(s) 133, 376
BstC8I GCNNGC 6 cut(s) 228, 441, 601, 605, 718, 912
BstDEI CTNAG 3 cut(s) 823, 833, 845
BstDSI CCRYGG 1 cut(s) 476
BstENI CCTNNNNNAGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 566
BstHHI GCGC 1 cut(s) 716
BstKTI GATC 3 cut(s) 87, 360, 673
BstMAI GTCTC 1 cut(s) 788
BstMBI GATC 3 cut(s) 84, 357, 670
BstMWI GCNNNNNNNGC 7 cut(s) 247, 487, 711, 722, 786, 855, 916
BstNI CCWGG 3 cut(s) 23, 41, 57
BstNSI RCATGY 5 cut(s) 34, 141, 645, 690, 841
BstPAI GACNNNNGTC 1 cut(s) 740
BstSCI CCNGG 3 cut(s) 21, 39, 55
BstSFI CTRYAG 2 cut(s) 201, 759
BstSLI GKGCMC 1 cut(s) 232
BstV1I GCAGC 4 cut(s) 712, 745, 842, 931
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 2 cut(s) 39, 428
BtgI CCRYGG 1 cut(s) 476
BtsCI GGATG 1 cut(s) 566
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 6 cut(s) 162, 210, 353, 738, 922, 941
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 6 cut(s) 228, 441, 601, 605, 718, 912
CaiI CAGNNNCTG 1 cut(s) 855
CciI TCATGA 1 cut(s) 115
CfoI GCGC 1 cut(s) 716
Cfr13I GGNCC 2 cut(s) 121, 337
Csp6I GTAC 3 cut(s) 28, 274, 818
CviQI GTAC 3 cut(s) 28, 274, 818
DdeI CTNAG 3 cut(s) 823, 833, 845
DpnI GATC 3 cut(s) 86, 359, 672
DpnII GATC 3 cut(s) 84, 357, 670
DraI TTTAAA 1 cut(s) 433
Eam1104I CTCTTC 1 cut(s) 822
EarI CTCTTC 1 cut(s) 822
Ecl136II GAGCTC 1 cut(s) 607
Eco130I CCWWGG 1 cut(s) 476
Eco147I AGGCCT 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 609
Eco47I GGWCC 2 cut(s) 121, 337
Eco53kI GAGCTC 1 cut(s) 607
Eco57I CTGAAG 2 cut(s) 98, 452
EcoICRI GAGCTC 1 cut(s) 607
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO109I RGGNCCY 1 cut(s) 337
EcoRII CCWGG 3 cut(s) 21, 39, 55
EcoT14I CCWWGG 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 476
FalI AAGNNNNNCTT 6 cut(s) 71, 103, 263, 295, 282, 314
FbaI TGATCA 1 cut(s) 357
Fnu4HI GCNGC 5 cut(s) 100, 726, 759, 856, 920
FokI GGATG 1 cut(s) 573
FriOI GRGCYC 1 cut(s) 609
Fsp4HI GCNGC 5 cut(s) 100, 726, 759, 856, 920
GlaI GCGC 1 cut(s) 715
GluI GCNGC 5 cut(s) 100, 726, 759, 856, 920
GsuI CTGGAG 1 cut(s) 866
HaeIII GGCC 2 cut(s) 39, 428
HhaI GCGC 1 cut(s) 716
Hin6I GCGC 1 cut(s) 714
HinP1I GCGC 1 cut(s) 714
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 6 cut(s) 403, 462, 472, 614, 904, 929
Hpy166II GTNNAC 2 cut(s) 634, 820
Hpy188I TCNGA 1 cut(s) 471
Hpy188III TCNNGA 4 cut(s) 70, 88, 116, 883
Hpy8I GTNNAC 2 cut(s) 634, 820
HpyAV CCTTC 3 cut(s) 194, 323, 476
HpyCH4III ACNGT 1 cut(s) 664
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 8 cut(s) 12, 34, 203, 250, 353, 454, 728, 761
HpyF10VI GCNNNNNNNGC 7 cut(s) 247, 487, 711, 722, 786, 855, 916
HpyF3I CTNAG 3 cut(s) 823, 833, 845
HpySE526I ACGT 1 cut(s) 129
HspAI GCGC 1 cut(s) 714
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 3 cut(s) 84, 357, 670
LguI GCTCTTC 1 cut(s) 822
LmnI GCTCC 4 cut(s) 168, 569, 871, 885
Lsp1109I GCAGC 4 cut(s) 712, 745, 842, 931
LweI GCATC 3 cut(s) 259, 551, 737
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 3 cut(s) 343, 733, 840
MalI GATC 3 cut(s) 86, 359, 672
MboI GATC 3 cut(s) 84, 357, 670
MboII GAAGA 7 cut(s) 58, 91, 94, 103, 338, 550, 839
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 2 cut(s) 232, 609
MluCI AATT 3 cut(s) 236, 321, 508
MlyI GAGTC 1 cut(s) 481
MnlI CCTC 9 cut(s) 100, 112, 296, 550, 578, 792, 802, 870, 894
MseI TTAA 6 cut(s) 219, 320, 432, 512, 752, 958
MspR9I CCNGG 3 cut(s) 23, 41, 57
MvaI CCWGG 3 cut(s) 23, 41, 57
MwoI GCNNNNNNNGC 7 cut(s) 247, 487, 711, 722, 786, 855, 916
NcoI CCATGG 1 cut(s) 476
NdeII GATC 3 cut(s) 84, 357, 670
NlaIV GGNNCC 2 cut(s) 308, 881
NmeAIII GCCGAG 2 cut(s) 701, 815
NmuCI GTSAC 1 cut(s) 733
NspI RCATGY 5 cut(s) 34, 141, 645, 690, 841
NspV TTCGAA 2 cut(s) 133, 376
PagI TCATGA 1 cut(s) 115
PceI AGGCCT 1 cut(s) 428
PciI ACATGT 2 cut(s) 641, 686
PciSI GCTCTTC 1 cut(s) 822
PfeI GAWTC 5 cut(s) 403, 462, 614, 904, 929
PflFI GACNNNGTC 1 cut(s) 529
PkrI GCNGC 5 cut(s) 101, 727, 760, 857, 921
PleI GAGTC 1 cut(s) 480
PpsI GAGTC 1 cut(s) 480
PpuMI RGGWCCY 1 cut(s) 337
PscI ACATGT 2 cut(s) 641, 686
PshAI GACNNNNGTC 1 cut(s) 740
Psp124BI GAGCTC 1 cut(s) 609
Psp5II RGGWCCY 1 cut(s) 337
Psp6I CCWGG 3 cut(s) 21, 39, 55
PspGI CCWGG 3 cut(s) 21, 39, 55
PspN4I GGNNCC 2 cut(s) 308, 881
PspPI GGNCC 2 cut(s) 121, 337
PspPPI RGGWCCY 1 cut(s) 337
PstI CTGCAG 2 cut(s) 205, 763
PstNI CAGNNNCTG 1 cut(s) 855
PsuI RGATCY 1 cut(s) 84
PsyI GACNNNGTC 1 cut(s) 529
RsaI GTAC 3 cut(s) 29, 275, 819
RsaNI GTAC 3 cut(s) 28, 274, 818
SacI GAGCTC 1 cut(s) 609
SapI GCTCTTC 1 cut(s) 822
SaqAI TTAA 6 cut(s) 219, 320, 432, 512, 752, 958
SatI GCNGC 5 cut(s) 100, 726, 759, 856, 920
Sau3AI GATC 3 cut(s) 84, 357, 670
Sau96I GGNCC 2 cut(s) 121, 337
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 3 cut(s) 23, 41, 57
SduI GDGCHC 2 cut(s) 232, 609
SfaNI GCATC 3 cut(s) 259, 551, 737
SfcI CTRYAG 2 cut(s) 201, 759
SfuI TTCGAA 2 cut(s) 133, 376
SinI GGWCC 2 cut(s) 121, 337
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 3 cut(s) 236, 321, 508
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 1 cut(s) 100
SstI GAGCTC 1 cut(s) 609
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 21, 39, 55
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 1 cut(s) 664
TaiI ACGT 1 cut(s) 132
TaqI TCGA 4 cut(s) 133, 301, 376, 744
TasI AATT 3 cut(s) 236, 321, 508
TatI WGTACW 3 cut(s) 27, 273, 817
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 5 cut(s) 403, 462, 614, 904, 929
Tru1I TTAA 6 cut(s) 219, 320, 432, 512, 752, 958
Tru9I TTAA 6 cut(s) 219, 320, 432, 512, 752, 958
TscAI CASTG 6 cut(s) 162, 210, 360, 738, 929, 941
TseFI GTSAC 1 cut(s) 733
TseI GCWGC 4 cut(s) 725, 758, 855, 919
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 4 cut(s) 17, 395, 876, 937
TspRI CASTG 6 cut(s) 162, 210, 360, 738, 929, 941
Tth111I GACNNNGTC 1 cut(s) 529
VpaK11BI GGWCC 2 cut(s) 121, 337
XagI CCTNNNNNAGG 1 cut(s) 188
XceI RCATGY 5 cut(s) 34, 141, 645, 690, 841
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.