FvH4_2g25590

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007402
Physical Location & Seq
Reverse (-)
20710010 .. 20711657
1648 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25590.t1

Sequence Viewer

Length: 981 bp
ATGAAGTTCTGCAAGAAATACGAGGAGTACATGCAAGGCCAAGAGAAGAAGCTACCTGAGGTTGGCTTCAAGAAGCTTAAGAAGATCTTGAAGAAATGCCGAAAGGATTTTCATTCGGAAGACGACATTAATAATCTTGCCGTACATGACCCCAGAACATGCCCTGACCATTGCCCAGTGTGTGATGGATCGTTTTTCCCTTCGCTGCTCAATGAAATGTCTGATGTGGTAGGCTTTTTCAATCAGAAAGCACAGAAATTGCTCGAGTACCATTTGGCTTCTGGGTTTCGCAAGTACATGCTTTTGTTCAAAGGCAAGCCTCAAGGAAACCATGTGGCTTTAATCCAAGAAGGCAAGGACCTTGTTACCTATGCACTCATCAACGCCATTGCGATCCGAAAAATACTCAAAAAATATGATAAGATTCATTACTCGAAGCAAGGGCAGGCCTTTAAGTCACAAGCGCAAAGTATGCACATTGAAATCCTTCAAAGTCCTTGGCTCTGCGAGCTTATGGCTTTCCACATCAATTTAAGGCGATCGAATATCAAATCGAGAAAGAGACCGGCCTTGTTTGAGGGATGCTCTCTTATAATGAATGATGAGAAGCCGACTCTCACCTGTGAACTCTTTGATTCTGTCAAGGTTGATATTGATTTGACATGTTCAATATGCCTGGACACAGTTTTTGATCCAGTTGCTCTCACTTGCGGTCATATATTCTGCTATATGTGTGCTTGCTCGGCTGCATCTGTGACCATTGTGGATGGGTTAAAGGCTGCCGAGTCTAAAGAAAAATGCCCTTTATGCCGAGAGGCAAAAGTGTACCAAGGTGCTGTACACCTGGATGAGCTTAGCTTTTTAATCAGCAGAAGCTGTCGCGAATACTGGGAGCAGCGCATTCAGACAGAAAAGGTGGAGAGGGTTCAGCAAGCAAAGCAGCACTGGGAATCACAATGTCGGTTATTCATGGGCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008219 GO:0009056 GO:0009057 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009627 GO:0009696 GO:0009697 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009987 GO:0010033 GO:0010035 GO:0010167 GO:0010337 GO:0010498 GO:0010565 GO:0010817 GO:0012501 GO:0014070 GO:0016020 GO:0016053 GO:0016567 GO:0016740 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019222 GO:0019438 GO:0019538 GO:0019752 GO:0019787 GO:0019941 GO:0030163 GO:0031323 GO:0032350 GO:0032446 GO:0032787 GO:0033554 GO:0034050 GO:0036211 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0042537 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061630 GO:0061659 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0071944 GO:0072330 GO:0080021 GO:0098542 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

327

Amino Acids

37.51

Weight (kDa)

8.69

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RING_XB3-XBAT31 PF24921 221 - 274 8.2e-08 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
zf-C3HC4 PF00097 222 - 270 1.9e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 593
AccII CGCG 1 cut(s) 882
AciI CCGC 1 cut(s) 711
AclWI GGATC 3 cut(s) 196, 388, 686
AfaI GTAC 6 cut(s) 29, 144, 269, 296, 827, 840
AfiI CCNNNNNNNGG 1 cut(s) 62
AflII CTTAAG 1 cut(s) 77
AflIII ACRYGT 1 cut(s) 662
AgsI TTSAA 7 cut(s) 70, 91, 241, 310, 482, 491, 669
AjnI CCWGG 2 cut(s) 675, 843
AluBI AGCT 6 cut(s) 52, 76, 511, 853, 858, 876
AluI AGCT 6 cut(s) 52, 76, 511, 853, 858, 876
Alw26I GTCTC 1 cut(s) 556
AlwI GGATC 3 cut(s) 196, 388, 686
AlwNI CAGNNNCTG 1 cut(s) 876
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 3 cut(s) 37, 447, 567
ApeKI GCWGC 5 cut(s) 205, 746, 779, 895, 940
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
AseI ATTAAT 1 cut(s) 129
Asp700I GAANNNNTTC 1 cut(s) 486
AspLEI GCGC 2 cut(s) 466, 900
AspS9I GGNCC 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 610
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 1 cut(s) 358
AxyI CCTNAGG 1 cut(s) 57
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 5 cut(s) 192, 733, 766, 907, 952
BccI CCATC 2 cut(s) 179, 761
BceAI ACGGC 1 cut(s) 125
BciT130I CCWGG 2 cut(s) 677, 845
BcoDI GTCTC 1 cut(s) 556
BfrI CTTAAG 1 cut(s) 77
BglII AGATCT 1 cut(s) 84
BisI GCNGC 5 cut(s) 206, 747, 780, 896, 941
BlpI GCTNAGC 1 cut(s) 854
BlsI GCNGC 5 cut(s) 207, 748, 781, 897, 942
Bme1390I CCNGG 2 cut(s) 677, 845
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 358
BmrFI CCNGG 2 cut(s) 677, 845
BmrI ACTGGG 3 cut(s) 170, 898, 955
BmsI GCATC 2 cut(s) 572, 758
BmuI ACTGGG 3 cut(s) 170, 898, 955
BpiI GAAGAC 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 569, 601
Bpu1102I GCTNAGC 1 cut(s) 854
BpuEI CTTGAG 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 556
BsaJI CCNNGG 2 cut(s) 497, 829
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse118I RCCGGY 1 cut(s) 565
Bse1I ACTGG 4 cut(s) 176, 695, 893, 950
Bse21I CCTNAGG 1 cut(s) 57
Bse3DI GCAATG 2 cut(s) 169, 387
BseBI CCWGG 2 cut(s) 677, 845
BseDI CCNNGG 2 cut(s) 497, 829
BseGI GGATG 3 cut(s) 587, 772, 853
BseLI CCNNNNNNNGG 1 cut(s) 62
BseMI GCAATG 2 cut(s) 169, 387
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 4 cut(s) 176, 695, 893, 950
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 5 cut(s) 192, 733, 766, 907, 952
Bsh1236I CGCG 1 cut(s) 882
Bsh1285I CGRYCG 1 cut(s) 542
BshFI GGCC 3 cut(s) 39, 449, 569
BsiEI CGRYCG 1 cut(s) 542
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 1 cut(s) 566
BslI CCNNNNNNNGG 1 cut(s) 62
BsmAI GTCTC 1 cut(s) 556
BsmI GAATGC 1 cut(s) 900
BsnI GGCC 3 cut(s) 39, 449, 569
Bso31I GGTCTC 1 cut(s) 556
BsoBI CYCGRG 1 cut(s) 263
Bsp1407I TGTACA 1 cut(s) 838
Bsp143I GATC 5 cut(s) 84, 188, 393, 539, 691
Bsp1720I GCTNAGC 1 cut(s) 854
Bsp68I TCGCGA 1 cut(s) 882
BspACI CCGC 1 cut(s) 711
BspANI GGCC 3 cut(s) 39, 449, 569
BspCNI CTCAG 1 cut(s) 49
BspFNI CGCG 1 cut(s) 882
BspPI GGATC 3 cut(s) 196, 388, 686
BspTI CTTAAG 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 556
BsrDI GCAATG 2 cut(s) 169, 387
BsrFI RCCGGY 1 cut(s) 565
BsrGI TGTACA 1 cut(s) 838
BsrI ACTGG 4 cut(s) 176, 695, 893, 950
BssAI RCCGGY 1 cut(s) 565
BssECI CCNNGG 2 cut(s) 497, 829
BssMI GATC 5 cut(s) 84, 188, 393, 539, 691
BssT1I CCWWGG 2 cut(s) 497, 829
Bst2UI CCWGG 2 cut(s) 677, 845
Bst4CI ACNGT 1 cut(s) 685
BstAFI CTTAAG 1 cut(s) 77
BstAPI GCANNNNNTGC 1 cut(s) 472
BstAUI TGTACA 1 cut(s) 838
BstC8I GCNNGC 5 cut(s) 317, 447, 509, 739, 933
BstDEI CTNAG 2 cut(s) 57, 854
BstF5I GGATG 3 cut(s) 587, 772, 853
BstFNI CGCG 1 cut(s) 882
BstHHI GCGC 2 cut(s) 466, 900
BstKTI GATC 5 cut(s) 87, 191, 396, 542, 694
BstMAI GTCTC 1 cut(s) 556
BstMBI GATC 5 cut(s) 84, 188, 393, 539, 691
BstMCI CGRYCG 1 cut(s) 542
BstMWI GCNNNNNNNGC 5 cut(s) 472, 508, 743, 807, 937
BstNI CCWGG 2 cut(s) 677, 845
BstNSI RCATGY 4 cut(s) 34, 162, 301, 666
BstSCI CCNGG 2 cut(s) 675, 843
BstUI CGCG 1 cut(s) 882
BstV1I GCAGC 5 cut(s) 192, 733, 766, 907, 952
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
Bsu36I CCTNAGG 1 cut(s) 57
BsuRI GGCC 3 cut(s) 39, 449, 569
BtsCI GGATG 3 cut(s) 587, 772, 853
BtsIMutI CAGTG 2 cut(s) 183, 943
BtuMI TCGCGA 1 cut(s) 882
Cac8I GCNNGC 5 cut(s) 317, 447, 509, 739, 933
CaiI CAGNNNCTG 1 cut(s) 876
CfoI GCGC 2 cut(s) 466, 900
Cfr10I RCCGGY 1 cut(s) 565
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 6 cut(s) 28, 143, 268, 295, 826, 839
CviAII CATG 7 cut(s) 31, 146, 159, 298, 332, 663, 970
CviQI GTAC 6 cut(s) 28, 143, 268, 295, 826, 839
DdeI CTNAG 2 cut(s) 57, 854
DpnI GATC 5 cut(s) 86, 190, 395, 541, 693
DpnII GATC 5 cut(s) 84, 188, 393, 539, 691
Eco130I CCWWGG 2 cut(s) 497, 829
Eco147I AGGCCT 1 cut(s) 449
Eco31I GGTCTC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 358
Eco81I CCTNAGG 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 2 cut(s) 675, 843
EcoT14I CCWWGG 2 cut(s) 497, 829
ErhI CCWWGG 2 cut(s) 497, 829
FaeI CATG 7 cut(s) 34, 149, 162, 301, 335, 666, 973
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FatI CATG 7 cut(s) 30, 145, 158, 297, 331, 662, 969
Fnu4HI GCNGC 5 cut(s) 206, 747, 780, 896, 941
FokI GGATG 3 cut(s) 594, 779, 860
Fsp4HI GCNGC 5 cut(s) 206, 747, 780, 896, 941
GlaI GCGC 2 cut(s) 465, 899
GluI GCNGC 5 cut(s) 206, 747, 780, 896, 941
HaeIII GGCC 3 cut(s) 39, 449, 569
HapII CCGG 1 cut(s) 566
HhaI GCGC 2 cut(s) 466, 900
Hin1II CATG 7 cut(s) 34, 149, 162, 301, 335, 666, 973
Hin6I GCGC 2 cut(s) 464, 898
HinP1I GCGC 2 cut(s) 464, 898
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 5 cut(s) 424, 613, 635, 785, 950
HpaII CCGG 1 cut(s) 566
HphI GGTGA 1 cut(s) 610
Hpy166II GTNNAC 3 cut(s) 626, 826, 841
Hpy188I TCNGA 5 cut(s) 118, 223, 246, 398, 906
Hpy188III TCNNGA 4 cut(s) 70, 88, 555, 881
Hpy8I GTNNAC 3 cut(s) 626, 826, 841
HpyAV CCTTC 3 cut(s) 210, 344, 497
HpyCH4III ACNGT 1 cut(s) 685
HpyCH4V TGCA 5 cut(s) 12, 34, 374, 475, 749
HpyF10VI GCNNNNNNNGC 5 cut(s) 472, 508, 743, 807, 937
HpyF3I CTNAG 2 cut(s) 57, 854
Hsp92II CATG 7 cut(s) 34, 149, 162, 301, 335, 666, 973
HspAI GCGC 2 cut(s) 464, 898
Kzo9I GATC 5 cut(s) 84, 188, 393, 539, 691
LmnI GCTCC 1 cut(s) 892
Lsp1109I GCAGC 5 cut(s) 192, 733, 766, 907, 952
LweI GCATC 2 cut(s) 572, 758
MaeIII GTNAC 3 cut(s) 364, 456, 754
MalI GATC 5 cut(s) 86, 190, 395, 541, 693
MboI GATC 5 cut(s) 84, 188, 393, 539, 691
MboII GAAGA 4 cut(s) 58, 94, 103, 131
MflI RGATCY 1 cut(s) 84
MluCI AATT 2 cut(s) 257, 529
MlyI GAGTC 2 cut(s) 607, 794
MnlI CCTC 6 cut(s) 16, 52, 330, 571, 808, 915
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 7 cut(s) 78, 129, 341, 453, 533, 773, 863
MslI CAYNNNNRTG 1 cut(s) 846
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 1 cut(s) 566
MspR9I CCNGG 2 cut(s) 677, 845
Mva1269I GAATGC 1 cut(s) 900
MvaI CCWGG 2 cut(s) 677, 845
MvnI CGCG 1 cut(s) 882
MwoI GCNNNNNNNGC 5 cut(s) 472, 508, 743, 807, 937
NdeII GATC 5 cut(s) 84, 188, 393, 539, 691
NlaIII CATG 7 cut(s) 34, 149, 162, 301, 335, 666, 973
NmeAIII GCCGAG 3 cut(s) 722, 808, 836
NmuCI GTSAC 2 cut(s) 456, 754
NruI TCGCGA 1 cut(s) 882
NspI RCATGY 4 cut(s) 34, 162, 301, 666
PaeR7I CTCGAG 1 cut(s) 263
PceI AGGCCT 1 cut(s) 449
PciI ACATGT 1 cut(s) 662
PctI GAATGC 1 cut(s) 900
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 3 cut(s) 424, 635, 950
PkrI GCNGC 5 cut(s) 207, 748, 781, 897, 942
Ple19I CGATCG 1 cut(s) 542
PleI GAGTC 2 cut(s) 607, 793
PpsI GAGTC 2 cut(s) 607, 793
PpuMI RGGWCCY 1 cut(s) 358
PscI ACATGT 1 cut(s) 662
PshBI ATTAAT 1 cut(s) 129
PsiI TTATAA 1 cut(s) 593
Psp5II RGGWCCY 1 cut(s) 358
Psp6I CCWGG 2 cut(s) 675, 843
PspGI CCWGG 2 cut(s) 675, 843
PspPI GGNCC 1 cut(s) 358
PspPPI RGGWCCY 1 cut(s) 358
PspXI VCTCGAGB 1 cut(s) 263
PstNI CAGNNNCTG 1 cut(s) 876
PsuI RGATCY 1 cut(s) 84
PvuI CGATCG 1 cut(s) 542
RruI TCGCGA 1 cut(s) 882
RsaI GTAC 6 cut(s) 29, 144, 269, 296, 827, 840
RsaNI GTAC 6 cut(s) 28, 143, 268, 295, 826, 839
RseI CAYNNNNRTG 1 cut(s) 846
SaqAI TTAA 7 cut(s) 78, 129, 341, 453, 533, 773, 863
SatI GCNGC 5 cut(s) 206, 747, 780, 896, 941
Sau3AI GATC 5 cut(s) 84, 188, 393, 539, 691
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 2 cut(s) 607, 794
ScrFI CCNGG 2 cut(s) 677, 845
SfaNI GCATC 2 cut(s) 572, 758
Sfr274I CTCGAG 1 cut(s) 263
SinI GGWCC 1 cut(s) 358
SlaI CTCGAG 1 cut(s) 263
SmiMI CAYNNNNRTG 1 cut(s) 846
SmlI CTYRAG 3 cut(s) 77, 263, 321
SmoI CTYRAG 3 cut(s) 77, 263, 321
Sse9I AATT 2 cut(s) 257, 529
SseBI AGGCCT 1 cut(s) 449
SsiI CCGC 1 cut(s) 711
StuI AGGCCT 1 cut(s) 449
StyD4I CCNGG 2 cut(s) 675, 843
StyI CCWWGG 2 cut(s) 497, 829
TaaI ACNGT 1 cut(s) 685
TaqI TCGA 4 cut(s) 264, 434, 542, 554
TasI AATT 2 cut(s) 257, 529
TatI WGTACW 3 cut(s) 27, 294, 838
TfiI GAWTC 3 cut(s) 424, 635, 950
Tru1I TTAA 7 cut(s) 78, 129, 341, 453, 533, 773, 863
Tru9I TTAA 7 cut(s) 78, 129, 341, 453, 533, 773, 863
TscAI CASTG 2 cut(s) 183, 950
TseFI GTSAC 2 cut(s) 456, 754
TseI GCWGC 5 cut(s) 205, 746, 779, 895, 940
Tsp45I GTSAC 2 cut(s) 456, 754
TspDTI ATGAA 6 cut(s) 17, 101, 228, 416, 611, 958
TspRI CASTG 2 cut(s) 183, 950
Vha464I CTTAAG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 358
VspI ATTAAT 1 cut(s) 129
XceI RCATGY 4 cut(s) 34, 162, 301, 666
XcmI CCANNNNNNNNNTGG 1 cut(s) 278
XhoI CTCGAG 1 cut(s) 263
XmnI GAANNNNTTC 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.