pycom03g22720

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007402
Physical Location & Seq
Reverse (-)
22775068 .. 22776560
1493 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22720.5

Sequence Viewer

Length: 765 bp
ATGTCTGCAGTGGTGGGTTTCTTCAATAAGCGCGCACAGAAATTGCTTGAACTCCATCTGTCTTCCGGCTTTCGTAAGTACATGTTTTGGTTCAAAGGCAAAGGTCGAGGGGGCCATGGTGCCTTGATCGAAGAAGGCAAGGACCTCGTGACTTATGCTCTGATCAACGCCGTTGCCATTCGGAAAATACTAAAGAAATATGATAAGATTCATTACTCGACGCAAGGACAGGCCTTCAAGTCGCAAGCTCAAAGCATGCACATTGGACTCCTTCAGAGCCCCTGGCTTTTTGAGCTCATGGCTTTTCACATCAATTTAAGGGAAACCAAGACTAAGTCGAGTAAGACACCGGCTCTGTTTGAGGGATGCTCCCTCAAGATTAATGATGGGAAACCATCTCTGGCCTGTGAGCTCTTTGATTCCGTTAAGCTTGATATCGATTTGACTTGTTCTATATGCTTGGACACGGTTTTTGATCCAGTTGCTCTCGCATGTGGTCACATATTCTGCTACATGTGTGCTTGCTCGGCCGCATCAGTGACTATTGTCGATGGATTGAAGTCCGCAGAGCCTAAAGAAAAATGCCCTCTATGCCGAGAGGCAAGAGTTTTCCAAGGCGCTGTACATTTAGAAGAGCTCAGCATGTTACTTAGCAGAAACTGCCGTGAATACTGGGAGCAGAGACTCCAGACTGAGAGGGTAGAGAGGGTTCGGCAAGCAAAACAGCACTGGGAATTCCAGTGCCGGGCATTCGTTGGCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008219 GO:0009056 GO:0009057 GO:0009058 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009627 GO:0009696 GO:0009697 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009987 GO:0010033 GO:0010035 GO:0010167 GO:0010337 GO:0010498 GO:0010565 GO:0010817 GO:0012501 GO:0014070 GO:0016020 GO:0016053 GO:0016567 GO:0016740 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019222 GO:0019438 GO:0019538 GO:0019752 GO:0019787 GO:0019941 GO:0030163 GO:0031323 GO:0032350 GO:0032446 GO:0032787 GO:0033554 GO:0034050 GO:0036211 GO:0042221 GO:0042445 GO:0042446 GO:0042493 GO:0042537 GO:0042742 GO:0043161 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046677 GO:0050789 GO:0050794 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061630 GO:0061659 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0071944 GO:0072330 GO:0080021 GO:0098542 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901615 GO:1901617 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

255

Amino Acids

28.77

Weight (kDa)

8.96

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 119
AccII CGCG 1 cut(s) 33
AciI CCGC 2 cut(s) 531, 564
AclWI GGATC 1 cut(s) 470
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 1 cut(s) 734
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 2 cut(s) 80, 624
AfiI CCNNNNNNNGG 1 cut(s) 745
AflIII ACRYGT 2 cut(s) 81, 513
AgsI TTSAA 5 cut(s) 25, 50, 94, 238, 559
AjnI CCWGG 1 cut(s) 281
AluBI AGCT 5 cut(s) 248, 295, 412, 430, 637
AluI AGCT 5 cut(s) 248, 295, 412, 430, 637
Alw21I GWGCWC 3 cut(s) 297, 414, 639
Alw26I GTCTC 1 cut(s) 676
AlwI GGATC 1 cut(s) 470
AlwNI CAGNNNCTG 1 cut(s) 660
AoxI GGCC 4 cut(s) 112, 231, 402, 528
ApoI RAATTY 1 cut(s) 734
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
AseI ATTAAT 1 cut(s) 381
AspLEI GCGC 3 cut(s) 33, 35, 620
AspS9I GGNCC 2 cut(s) 112, 142
AsuC2I CCSGG 1 cut(s) 746
AvaII GGWCC 1 cut(s) 142
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 4 cut(s) 281, 297, 414, 639
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 3 cut(s) 297, 414, 639
BccI CCATC 4 cut(s) 63, 380, 403, 545
BceAI ACGGC 2 cut(s) 155, 648
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 1 cut(s) 283
BclI TGATCA 1 cut(s) 162
BcnI CCSGG 1 cut(s) 746
BcoDI GTCTC 1 cut(s) 676
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 621
BisI GCNGC 1 cut(s) 531
BlpI GCTNAGC 1 cut(s) 638
BlsI GCNGC 1 cut(s) 532
Bme1390I CCNGG 2 cut(s) 283, 746
Bme18I GGWCC 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 112, 142
BmiI GGNNCC 2 cut(s) 113, 121
BmrFI CCNGG 2 cut(s) 283, 746
BmrI ACTGGG 2 cut(s) 682, 739
BmsI GCATC 2 cut(s) 356, 542
BmuI ACTGGG 2 cut(s) 682, 739
BoxI GACNNNNGTC 1 cut(s) 545
BpiI GAAGAC 1 cut(s) 54
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 1 cut(s) 671
Bpu1102I GCTNAGC 1 cut(s) 638
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 746
Bsa29I ATCGAT 1 cut(s) 438
BsaJI CCNNGG 3 cut(s) 115, 281, 613
Bsc4I CCNNNNNNNGG 1 cut(s) 745
Bse118I RCCGGY 1 cut(s) 349
Bse1I ACTGG 4 cut(s) 479, 677, 734, 739
BseBI CCWGG 1 cut(s) 283
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 3 cut(s) 115, 281, 613
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 1 cut(s) 745
BseMII CTCAG 2 cut(s) 652, 684
BseNI ACTGG 4 cut(s) 479, 677, 734, 739
BsePI GCGCGC 1 cut(s) 31
BseX3I CGGCCG 1 cut(s) 528
Bsh1236I CGCG 1 cut(s) 33
Bsh1285I CGRYCG 1 cut(s) 531
BshFI GGCC 4 cut(s) 114, 233, 404, 530
BshNI GGYRCC 1 cut(s) 119
BshVI ATCGAT 1 cut(s) 438
BsiEI CGRYCG 1 cut(s) 531
BsiHKAI GWGCWC 3 cut(s) 297, 414, 639
BsiSI CCGG 3 cut(s) 66, 350, 745
BslI CCNNNNNNNGG 1 cut(s) 745
BsmAI GTCTC 1 cut(s) 676
BsmI GAATGC 1 cut(s) 749
BsnI GGCC 4 cut(s) 114, 233, 404, 530
Bsp1286I GDGCHC 4 cut(s) 281, 297, 414, 639
Bsp1407I TGTACA 1 cut(s) 622
Bsp143I GATC 3 cut(s) 126, 162, 475
Bsp1720I GCTNAGC 1 cut(s) 638
Bsp19I CCATGG 1 cut(s) 115
BspACI CCGC 2 cut(s) 531, 564
BspANI GGCC 4 cut(s) 114, 233, 404, 530
BspCNI CTCAG 2 cut(s) 651, 685
BspDI ATCGAT 1 cut(s) 438
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 113, 121
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 1 cut(s) 470
BspQI GCTCTTC 1 cut(s) 627
BspT107I GGYRCC 1 cut(s) 119
BsrFI RCCGGY 1 cut(s) 349
BsrGI TGTACA 1 cut(s) 622
BsrI ACTGG 4 cut(s) 479, 677, 734, 739
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 3 cut(s) 115, 281, 613
BssHII GCGCGC 1 cut(s) 31
BssMI GATC 3 cut(s) 126, 162, 475
BssSI CACGAG 1 cut(s) 146
BssT1I CCWWGG 2 cut(s) 115, 613
Bst2BI CACGAG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 1 cut(s) 469
Bst6I CTCTTC 1 cut(s) 627
BstAPI GCANNNNNTGC 1 cut(s) 660
BstAUI TGTACA 1 cut(s) 622
BstC8I GCNNGC 5 cut(s) 33, 246, 257, 523, 717
BstDEI CTNAG 4 cut(s) 333, 638, 650, 693
BstDSI CCRYGG 1 cut(s) 115
BstF5I GGATG 1 cut(s) 371
BstFNI CGCG 1 cut(s) 33
BstH2I RGCGCY 1 cut(s) 621
BstHHI GCGC 3 cut(s) 33, 35, 620
BstKTI GATC 3 cut(s) 129, 165, 478
BstMAI GTCTC 1 cut(s) 676
BstMBI GATC 3 cut(s) 126, 162, 475
BstMCI CGRYCG 1 cut(s) 531
BstMWI GCNNNNNNNGC 4 cut(s) 292, 527, 591, 660
BstNI CCWGG 1 cut(s) 283
BstNSI RCATGY 5 cut(s) 85, 259, 495, 517, 646
BstPAI GACNNNNGTC 1 cut(s) 545
BstSCI CCNGG 2 cut(s) 281, 744
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 1 cut(s) 33
BstV2I GAAGAC 1 cut(s) 54
BstZI CGGCCG 1 cut(s) 528
Bsu15I ATCGAT 1 cut(s) 438
BsuRI GGCC 4 cut(s) 114, 233, 404, 530
BsuTUI ATCGAT 1 cut(s) 438
BtgI CCRYGG 1 cut(s) 115
BtsCI GGATG 1 cut(s) 371
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 4 cut(s) 15, 543, 727, 746
Cac8I GCNNGC 5 cut(s) 33, 246, 257, 523, 717
CaiI CAGNNNCTG 1 cut(s) 660
CfoI GCGC 3 cut(s) 33, 35, 620
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 112, 142
ClaI ATCGAT 1 cut(s) 438
CseI GACGC 1 cut(s) 229
Csp6I GTAC 2 cut(s) 79, 623
CviAII CATG 7 cut(s) 82, 116, 256, 298, 492, 514, 643
CviQI GTAC 2 cut(s) 79, 623
DdeI CTNAG 4 cut(s) 333, 638, 650, 693
DpnI GATC 3 cut(s) 128, 164, 477
DpnII GATC 3 cut(s) 126, 162, 475
EaeI YGGCCR 1 cut(s) 528
EagI CGGCCG 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Ecl136II GAGCTC 3 cut(s) 295, 412, 637
EclXI CGGCCG 1 cut(s) 528
Eco130I CCWWGG 2 cut(s) 115, 613
Eco147I AGGCCT 1 cut(s) 233
Eco24I GRGCYC 4 cut(s) 281, 297, 414, 639
Eco32I GATATC 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 142
Eco52I CGGCCG 1 cut(s) 528
Eco53kI GAGCTC 3 cut(s) 295, 412, 637
Eco57I CTGAAG 1 cut(s) 257
EcoICRI GAGCTC 3 cut(s) 295, 412, 637
EcoO109I RGGNCCY 1 cut(s) 142
EcoRI GAATTC 1 cut(s) 734
EcoRII CCWGG 1 cut(s) 281
EcoRV GATATC 1 cut(s) 436
EcoT14I CCWWGG 2 cut(s) 115, 613
EcoT38I GRGCYC 4 cut(s) 281, 297, 414, 639
ErhI CCWWGG 2 cut(s) 115, 613
FaeI CATG 7 cut(s) 85, 119, 259, 301, 495, 517, 646
FatI CATG 7 cut(s) 81, 115, 255, 297, 491, 513, 642
FbaI TGATCA 1 cut(s) 162
Fnu4HI GCNGC 1 cut(s) 531
FokI GGATG 1 cut(s) 378
FriOI GRGCYC 4 cut(s) 281, 297, 414, 639
Fsp4HI GCNGC 1 cut(s) 531
GlaI GCGC 3 cut(s) 32, 34, 619
GluI GCNGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 671
HaeII RGCGCY 1 cut(s) 621
HaeIII GGCC 4 cut(s) 114, 233, 404, 530
HapII CCGG 3 cut(s) 66, 350, 745
HgaI GACGC 1 cut(s) 229
HhaI GCGC 3 cut(s) 33, 35, 620
Hin1II CATG 7 cut(s) 85, 119, 259, 301, 495, 517, 646
Hin6I GCGC 3 cut(s) 31, 33, 618
HinP1I GCGC 3 cut(s) 31, 33, 618
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 4 cut(s) 208, 267, 419, 684
HpaII CCGG 3 cut(s) 66, 350, 745
Hpy188I TCNGA 3 cut(s) 162, 183, 276
Hpy188III TCNNGA 3 cut(s) 148, 376, 688
Hpy99I CGWCG 1 cut(s) 223
HpyAV CCTTC 3 cut(s) 128, 244, 281
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4V TGCA 2 cut(s) 8, 259
HpyF10VI GCNNNNNNNGC 4 cut(s) 292, 527, 591, 660
HpyF3I CTNAG 4 cut(s) 333, 638, 650, 693
Hsp92II CATG 7 cut(s) 85, 119, 259, 301, 495, 517, 646
HspAI GCGC 3 cut(s) 31, 33, 618
Ksp22I TGATCA 1 cut(s) 162
Kzo9I GATC 3 cut(s) 126, 162, 475
LguI GCTCTTC 1 cut(s) 627
LmnI GCTCC 2 cut(s) 374, 676
LweI GCATC 2 cut(s) 356, 542
MaeIII GTNAC 4 cut(s) 148, 497, 538, 645
MalI GATC 3 cut(s) 128, 164, 477
MboI GATC 3 cut(s) 126, 162, 475
MboII GAAGA 4 cut(s) 13, 54, 143, 644
MhlI GDGCHC 4 cut(s) 281, 297, 414, 639
MluCI AATT 3 cut(s) 41, 313, 734
MlyI GAGTC 2 cut(s) 261, 678
MnlI CCTC 8 cut(s) 101, 155, 355, 383, 592, 597, 690, 699
MseI TTAA 3 cut(s) 317, 381, 426
MspI CCGG 3 cut(s) 66, 350, 745
MspR9I CCNGG 2 cut(s) 283, 746
Mva1269I GAATGC 1 cut(s) 749
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 4 cut(s) 292, 527, 591, 660
NciI CCSGG 1 cut(s) 746
NcoI CCATGG 1 cut(s) 115
NdeII GATC 3 cut(s) 126, 162, 475
NlaIII CATG 7 cut(s) 85, 119, 259, 301, 495, 517, 646
NlaIV GGNNCC 2 cut(s) 113, 121
NmeAIII GCCGAG 2 cut(s) 506, 620
NmuCI GTSAC 3 cut(s) 148, 497, 538
NspI RCATGY 5 cut(s) 85, 259, 495, 517, 646
PaeI GCATGC 1 cut(s) 259
PauI GCGCGC 1 cut(s) 31
PceI AGGCCT 1 cut(s) 233
PciI ACATGT 2 cut(s) 81, 513
PciSI GCTCTTC 1 cut(s) 627
PctI GAATGC 1 cut(s) 749
PfeI GAWTC 2 cut(s) 208, 419
PflFI GACNNNGTC 1 cut(s) 334
PkrI GCNGC 1 cut(s) 532
PleI GAGTC 2 cut(s) 261, 678
PpsI GAGTC 2 cut(s) 261, 678
PpuMI RGGWCCY 1 cut(s) 142
PscI ACATGT 2 cut(s) 81, 513
PshAI GACNNNNGTC 1 cut(s) 545
PshBI ATTAAT 1 cut(s) 381
Psp124BI GAGCTC 3 cut(s) 297, 414, 639
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 2 cut(s) 113, 121
PspPI GGNCC 2 cut(s) 112, 142
PspPPI RGGWCCY 1 cut(s) 142
PsrI GAACNNNNNNTAC 2 cut(s) 693, 725
PstI CTGCAG 1 cut(s) 10
PstNI CAGNNNCTG 1 cut(s) 660
PsyI GACNNNGTC 1 cut(s) 334
PteI GCGCGC 1 cut(s) 31
RsaI GTAC 2 cut(s) 80, 624
RsaNI GTAC 2 cut(s) 79, 623
SacI GAGCTC 3 cut(s) 297, 414, 639
SapI GCTCTTC 1 cut(s) 627
SaqAI TTAA 3 cut(s) 317, 381, 426
SatI GCNGC 1 cut(s) 531
Sau3AI GATC 3 cut(s) 126, 162, 475
Sau96I GGNCC 2 cut(s) 112, 142
SchI GAGTC 2 cut(s) 261, 678
ScrFI CCNGG 2 cut(s) 283, 746
SduI GDGCHC 4 cut(s) 281, 297, 414, 639
SetI ASST 7 cut(s) 106, 147, 250, 297, 414, 432, 639
SfaNI GCATC 2 cut(s) 356, 542
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 1 cut(s) 142
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
SphI GCATGC 1 cut(s) 259
Sse9I AATT 3 cut(s) 41, 313, 734
SseBI AGGCCT 1 cut(s) 233
SsiI CCGC 2 cut(s) 531, 564
SstI GAGCTC 3 cut(s) 297, 414, 639
StuI AGGCCT 1 cut(s) 233
StyD4I CCNGG 2 cut(s) 281, 744
StyI CCWWGG 2 cut(s) 115, 613
TaaI ACNGT 1 cut(s) 469
TaqI TCGA 6 cut(s) 106, 129, 218, 338, 438, 549
TasI AATT 3 cut(s) 41, 313, 734
TatI WGTACW 2 cut(s) 78, 622
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 2 cut(s) 208, 419
Tru1I TTAA 3 cut(s) 317, 381, 426
Tru9I TTAA 3 cut(s) 317, 381, 426
TscAI CASTG 4 cut(s) 15, 543, 734, 746
TseFI GTSAC 3 cut(s) 148, 497, 538
Tsp45I GTSAC 3 cut(s) 148, 497, 538
TspDTI ATGAA 1 cut(s) 200
TspGWI ACGGA 1 cut(s) 412
TspRI CASTG 4 cut(s) 15, 543, 734, 746
Tth111I GACNNNGTC 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 142
VspI ATTAAT 1 cut(s) 381
XapI RAATTY 1 cut(s) 734
XceI RCATGY 5 cut(s) 85, 259, 495, 517, 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.