AT1G05310

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012811
Physical Location & Seq
Reverse (-)
1550503 .. 1552587
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05310.1

Sequence Viewer

Length: 1182 bp
ATGAAAATAATCTCATTATCCATATCCATAGGTATCGCAATAATTGCCGTTCTGGCATCTAAAACTCTCTTTAAAACACATCCTGAAGCTTTTGGTATCAAAGCAATATCATATTCATTCAAGAAATCCTTATGTGATCACCATCACCATCATCATCATCATCATCATCACCACCACCGGCATAAACCTTCGGATACCAAAAGAAAGGTATCAATATGCGACGATTTCCCTAAAAATATACCTCCATTGGACACCGACACAACATCCTATCTCTGCGTGGATAAGAACGGTTGCTGCAATTTCACGACGGTGCAATCGGCCGTGGACGCAGTGGGGAATTTCAGTCAAAGAAGGAACGTGATTTGGATAAACTCCGGCATGTACTATGAAAAAGTGGTGATTCCGAAGACTAAACCAAACATAACGTTGCAAGGACAAGGCTTCGACATTACAGCCATTGCGTGGAACGACACTGCCTACTCAGCTAATGGCACATTTTATTGTGCCACGGTCCAAGTCTTTGGTTCTCAGTTCGTCGCTAAGAACATCAGTTTCATGAACGTGGCACCGATACCAAAGCCAGGAGACGTAGGAGCTCAGGCGGTGGCAATAAGAATCGCCGGAGATGAATCAGCGTTTGTGGGATGCGGTTTCTTCGGAGCACAAGACACTCTTCATGACGATAGAGGTCGTCATTACTTCAAGGATTGTTACATTCAAGGATCCATTGATTTCATCTTCGGCAACGCTAAGTCTCTCTATCAGGACTGTCGAATAATATCAATGGCTAATCAATTGAGCCCAGGTTCAAAAGCAGTTAACGGAGCCGTGACTGCAAATGGGCGTAGCTCCAAGGACGAGAACAGTGGCTTCTCCTTCGTCAACTGCACAATCGGTGGAACTGGTCACGTGTGGCTAGGTCGTGCATGGCGGCCTTACTCACGTGTGGTTTTCGTTTCCACTACTATGACCGATGTTATCGCACCCGAAGGCTGGAACAACTTCAACGATCCCTCTAGGGACGCGACTATATTTTATGGAGAGTATAATTGTTCGGGTCCAGGAGCAGATATGTCAAAGAGAGCACCATATGTTCAAAAACTTAATGAGACTCAAGTTGCTCTATTAATTAACACGTCTTTTATTGATGGAGATCAATGGTTACAGTTTTCTGACCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

393

Amino Acids

43.35

Weight (kDa)

8.22

Isoelectric Point (pI)

26.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 92 - 384 3.1e-73 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012811)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05310
fragaria_vesca FvH4_6g39490
malus_domestica MD09G1139900.v1.1 MD17G1129300.v1.1
prunus_persica Prupe.3G185700_v2.0.a1 Prupe.3G185700_v2.0.a1
pyrus_communis pycom09g06080 pycom17g12170
rosa_chinensis RchiOBHm_Chr2g0153521
rosa_laevigata RLG00000020705
rosa_multiflora Rmu_sc0003976.1_g000011
rosa_roxburghii Rroxscaffold_2G00094960
rosa_rugosa Rorug02G0438800
rosa_samantha Rh2AG502700 Rh2BG513500 Rh2CG488500 Rh2DG525200
rosa_wichuraiana Rw2G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 565
AccB7I CCANNNNNTGG 1 cut(s) 462
AccII CGCG 1 cut(s) 1025
AciI CCGC 3 cut(s) 602, 648, 931
AclI AACGTT 1 cut(s) 425
AclWI GGATC 3 cut(s) 717, 730, 1004
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 1 cut(s) 337
AcuI CTGAAG 1 cut(s) 105
AcvI CACGTG 2 cut(s) 910, 944
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 3 cut(s) 462, 581, 993
AflIII ACRYGT 3 cut(s) 909, 943, 1134
AgsI TTSAA 6 cut(s) 121, 703, 719, 810, 1006, 1097
AjiI CACGTC 1 cut(s) 1137
AjnI CCWGG 3 cut(s) 580, 802, 1060
AleI CACNNNNGTG 1 cut(s) 308
AluBI AGCT 4 cut(s) 89, 485, 596, 849
AluI AGCT 4 cut(s) 89, 485, 596, 849
Alw21I GWGCWC 3 cut(s) 598, 664, 1087
Alw26I GTCTC 3 cut(s) 579, 759, 1103
AlwI GGATC 3 cut(s) 717, 730, 1004
AoxI GGCC 2 cut(s) 318, 932
ApeKI GCWGC 1 cut(s) 294
ApoI RAATTY 1 cut(s) 337
AseI ATTAAT 1 cut(s) 1127
Asp700I GAANNNNTTC 1 cut(s) 1001
AspS9I GGNCC 2 cut(s) 511, 1058
AsuHPI GGTGA 4 cut(s) 131, 137, 161, 409
AvaII GGWCC 2 cut(s) 511, 1058
BamHI GGATCC 1 cut(s) 722
BanI GGYRCC 1 cut(s) 565
BanII GRGCYC 2 cut(s) 598, 803
BbrPI CACGTG 2 cut(s) 910, 944
BbsI GAAGAC 1 cut(s) 413
Bbv12I GWGCWC 3 cut(s) 598, 664, 1087
BbvI GCAGC 1 cut(s) 281
BccI CCATC 3 cut(s) 150, 156, 1142
BceAI ACGGC 3 cut(s) 32, 305, 812
BciT130I CCWGG 3 cut(s) 582, 804, 1062
BciVI GTATCC 1 cut(s) 187
BclI TGATCA 1 cut(s) 136
BcoDI GTCTC 3 cut(s) 579, 759, 1103
BfaI CTAG 2 cut(s) 917, 1017
BfuI GTATCC 1 cut(s) 187
BglI GCCNNNNNGGC 1 cut(s) 53
BisI GCNGC 2 cut(s) 295, 932
BlsI GCNGC 2 cut(s) 296, 933
Bme1390I CCNGG 3 cut(s) 582, 804, 1062
Bme18I GGWCC 2 cut(s) 511, 1058
BmgBI CACGTC 1 cut(s) 1137
BmgT120I GGNCC 2 cut(s) 511, 1058
BmiI GGNNCC 4 cut(s) 567, 724, 826, 1059
BmrFI CCNGG 3 cut(s) 582, 804, 1062
BmsI GCATC 2 cut(s) 65, 635
BpiI GAAGAC 1 cut(s) 413
Bpu10I CCTNAGC 1 cut(s) 597
BpuEI CTTGAG 1 cut(s) 1098
BsaAI YACGTR 2 cut(s) 910, 944
BsaBI GATNNNNATC 2 cut(s) 141, 1152
BsaJI CCNNGG 4 cut(s) 321, 507, 802, 852
Bsc4I CCNNNNNNNGG 3 cut(s) 462, 581, 993
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 1 cut(s) 907
Bse3DI GCAATG 1 cut(s) 456
Bse8I GATNNNNATC 2 cut(s) 141, 1152
BseBI CCWGG 3 cut(s) 582, 804, 1062
BseDI CCNNGG 4 cut(s) 321, 507, 802, 852
BseGI GGATG 3 cut(s) 79, 263, 650
BseJI GATNNNNATC 2 cut(s) 141, 1152
BseLI CCNNNNNNNGG 3 cut(s) 462, 581, 993
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 3 cut(s) 495, 542, 611
BseNI ACTGG 1 cut(s) 907
BseX3I CGGCCG 1 cut(s) 318
BseXI GCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 871
Bsh1236I CGCG 1 cut(s) 1025
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 2 cut(s) 320, 934
BshNI GGYRCC 1 cut(s) 565
BsiEI CGRYCG 1 cut(s) 321
BsiHKAI GWGCWC 3 cut(s) 598, 664, 1087
BsiSI CCGG 3 cut(s) 178, 375, 621
BslFI GGGAC 1 cut(s) 1034
BslI CCNNNNNNNGG 3 cut(s) 462, 581, 993
BsmAI GTCTC 3 cut(s) 579, 759, 1103
BsmBI CGTCTC 1 cut(s) 579
BsmFI GGGAC 1 cut(s) 1034
BsnI GGCC 2 cut(s) 320, 934
Bsp1286I GDGCHC 4 cut(s) 598, 664, 803, 1087
Bsp143I GATC 4 cut(s) 136, 722, 1009, 1153
BspACI CCGC 3 cut(s) 602, 648, 931
BspANI GGCC 2 cut(s) 320, 934
BspCNI CTCAG 3 cut(s) 494, 541, 610
BspFNI CGCG 1 cut(s) 1025
BspHI TCATGA 2 cut(s) 555, 676
BspLI GGNNCC 4 cut(s) 567, 724, 826, 1059
BspPI GGATC 3 cut(s) 717, 730, 1004
BspT107I GGYRCC 1 cut(s) 565
BsrDI GCAATG 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 177
BsrI ACTGG 1 cut(s) 907
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 4 cut(s) 321, 507, 802, 852
BssMI GATC 4 cut(s) 136, 722, 1009, 1153
BssT1I CCWWGG 1 cut(s) 852
Bst2UI CCWGG 3 cut(s) 582, 804, 1062
Bst4CI ACNGT 6 cut(s) 290, 310, 511, 770, 866, 1167
Bst6I CTCTTC 1 cut(s) 678
BstAPI GCANNNNNTGC 1 cut(s) 44
BstBAI YACGTR 2 cut(s) 910, 944
BstDEI CTNAG 5 cut(s) 481, 528, 540, 597, 750
BstDSI CCRYGG 2 cut(s) 321, 507
BstF5I GGATG 3 cut(s) 79, 263, 650
BstFNI CGCG 1 cut(s) 1025
BstKTI GATC 4 cut(s) 139, 725, 1012, 1156
BstMAI GTCTC 3 cut(s) 579, 759, 1103
BstMBI GATC 4 cut(s) 136, 722, 1009, 1153
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 5 cut(s) 44, 53, 326, 482, 833
BstNI CCWGG 3 cut(s) 582, 804, 1062
BstNSI RCATGY 1 cut(s) 382
BstSCI CCNGG 3 cut(s) 580, 802, 1060
BstUI CGCG 1 cut(s) 1025
BstV1I GCAGC 1 cut(s) 281
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 1 cut(s) 722
BstXI CCANNNNNNTGG 1 cut(s) 521
BstYI RGATCY 1 cut(s) 722
BstZI CGGCCG 1 cut(s) 318
BsuI GTATCC 1 cut(s) 187
BsuRI GGCC 2 cut(s) 320, 934
BtgI CCRYGG 2 cut(s) 321, 507
BtrI CACGTC 1 cut(s) 1137
BtsCI GGATG 3 cut(s) 79, 263, 650
BtsI GCAGTG 2 cut(s) 336, 471
BtsIMutI CAGTG 3 cut(s) 336, 471, 871
CciI TCATGA 2 cut(s) 555, 676
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 2 cut(s) 511, 1058
CseI GACGC 2 cut(s) 335, 1031
Csp6I GTAC 1 cut(s) 382
CviAII CATG 4 cut(s) 379, 556, 677, 927
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 5 cut(s) 481, 528, 540, 597, 750
DpnI GATC 4 cut(s) 138, 724, 1011, 1155
DpnII GATC 4 cut(s) 136, 722, 1009, 1153
DraI TTTAAA 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 318
EagI CGGCCG 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
Ecl136II GAGCTC 1 cut(s) 596
EclXI CGGCCG 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 852
Eco24I GRGCYC 2 cut(s) 598, 803
Eco47I GGWCC 2 cut(s) 511, 1058
Eco52I CGGCCG 1 cut(s) 318
Eco53kI GAGCTC 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 105
Eco72I CACGTG 2 cut(s) 910, 944
EcoICRI GAGCTC 1 cut(s) 596
EcoRII CCWGG 3 cut(s) 580, 802, 1060
EcoT14I CCWWGG 1 cut(s) 852
EcoT38I GRGCYC 2 cut(s) 598, 803
ErhI CCWWGG 1 cut(s) 852
Esp3I CGTCTC 1 cut(s) 579
FaeI CATG 4 cut(s) 382, 559, 680, 930
FalI AAGNNNNNCTT 4 cut(s) 113, 145, 657, 689
FaqI GGGAC 1 cut(s) 1034
FatI CATG 4 cut(s) 378, 555, 676, 926
FauNDI CATATG 1 cut(s) 1090
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 2 cut(s) 295, 932
FokI GGATG 3 cut(s) 66, 250, 657
FriOI GRGCYC 2 cut(s) 598, 803
Fsp4HI GCNGC 2 cut(s) 295, 932
FspBI CTAG 2 cut(s) 917, 1017
GluI GCNGC 2 cut(s) 295, 932
HaeIII GGCC 2 cut(s) 320, 934
HapII CCGG 3 cut(s) 178, 375, 621
HgaI GACGC 2 cut(s) 335, 1031
Hin1II CATG 4 cut(s) 382, 559, 680, 930
HincII GTYRAC 2 cut(s) 820, 883
HindII GTYRAC 2 cut(s) 820, 883
HindIII AAGCTT 1 cut(s) 87
HinfI GANTC 4 cut(s) 400, 615, 629, 1111
HpaI GTTAAC 1 cut(s) 820
HpaII CCGG 3 cut(s) 178, 375, 621
HphI GGTGA 4 cut(s) 131, 137, 161, 409
Hpy166II GTNNAC 3 cut(s) 325, 820, 883
Hpy188I TCNGA 4 cut(s) 193, 405, 659, 1174
Hpy188III TCNNGA 6 cut(s) 83, 121, 304, 556, 677, 764
Hpy8I GTNNAC 3 cut(s) 325, 820, 883
Hpy99I CGWCG 3 cut(s) 224, 310, 539
HpyAV CCTTC 4 cut(s) 198, 345, 886, 983
HpyCH4III ACNGT 6 cut(s) 290, 310, 511, 770, 866, 1167
HpyCH4IV ACGT 7 cut(s) 357, 425, 561, 588, 909, 943, 1136
HpyCH4V TGCA 6 cut(s) 297, 313, 430, 836, 888, 926
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 53, 326, 482, 833
HpyF3I CTNAG 5 cut(s) 481, 528, 540, 597, 750
HpySE526I ACGT 7 cut(s) 357, 425, 561, 588, 909, 943, 1136
Hsp92II CATG 4 cut(s) 382, 559, 680, 930
Ksp22I TGATCA 1 cut(s) 136
KspAI GTTAAC 1 cut(s) 820
Kzo9I GATC 4 cut(s) 136, 722, 1009, 1153
LmnI GCTCC 5 cut(s) 593, 659, 824, 854, 1064
Lsp1109I GCAGC 1 cut(s) 281
LweI GCATC 2 cut(s) 65, 635
MaeI CTAG 2 cut(s) 917, 1017
MaeII ACGT 7 cut(s) 357, 425, 561, 588, 909, 943, 1136
MaeIII GTNAC 4 cut(s) 710, 829, 905, 1161
MalI GATC 4 cut(s) 138, 724, 1011, 1155
MboI GATC 4 cut(s) 136, 722, 1009, 1153
MboII GAAGA 4 cut(s) 418, 646, 665, 730
MfeI CAATTG 1 cut(s) 794
MflI RGATCY 1 cut(s) 722
MhlI GDGCHC 4 cut(s) 598, 664, 803, 1087
MluCI AATT 6 cut(s) 42, 298, 337, 794, 1048, 1128
MlyI GAGTC 1 cut(s) 1105
MnlI CCTC 3 cut(s) 252, 680, 1024
MroXI GAANNNNTTC 1 cut(s) 1001
MseI TTAA 6 cut(s) 72, 819, 1104, 1127, 1131, 1180
MslI CAYNNNNRTG 3 cut(s) 308, 560, 965
MspI CCGG 3 cut(s) 178, 375, 621
MspR9I CCNGG 3 cut(s) 582, 804, 1062
MunI CAATTG 1 cut(s) 794
MvaI CCWGG 3 cut(s) 582, 804, 1062
MvnI CGCG 1 cut(s) 1025
MwoI GCNNNNNNNGC 5 cut(s) 44, 53, 326, 482, 833
NdeI CATATG 1 cut(s) 1090
NdeII GATC 4 cut(s) 136, 722, 1009, 1153
NlaIII CATG 4 cut(s) 382, 559, 680, 930
NlaIV GGNNCC 4 cut(s) 567, 724, 826, 1059
NmuCI GTSAC 2 cut(s) 829, 905
NspI RCATGY 1 cut(s) 382
OliI CACNNNNGTG 1 cut(s) 308
PacI TTAATTAA 1 cut(s) 1131
PagI TCATGA 2 cut(s) 555, 676
PdmI GAANNNNTTC 1 cut(s) 1001
PfeI GAWTC 3 cut(s) 400, 615, 629
PflMI CCANNNNNTGG 1 cut(s) 462
PfoI TCCNGGA 1 cut(s) 1060
PkrI GCNGC 2 cut(s) 296, 933
PleI GAGTC 1 cut(s) 1105
PmaCI CACGTG 2 cut(s) 910, 944
PmlI CACGTG 2 cut(s) 910, 944
PpsI GAGTC 1 cut(s) 1105
Ppu21I YACGTR 2 cut(s) 910, 944
PshBI ATTAAT 1 cut(s) 1127
Psp124BI GAGCTC 1 cut(s) 598
Psp1406I AACGTT 1 cut(s) 425
Psp6I CCWGG 3 cut(s) 580, 802, 1060
PspCI CACGTG 2 cut(s) 910, 944
PspGI CCWGG 3 cut(s) 580, 802, 1060
PspN4I GGNNCC 4 cut(s) 567, 724, 826, 1059
PspPI GGNCC 2 cut(s) 511, 1058
PsuI RGATCY 1 cut(s) 722
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
RseI CAYNNNNRTG 3 cut(s) 308, 560, 965
SacI GAGCTC 1 cut(s) 598
SaqAI TTAA 6 cut(s) 72, 819, 1104, 1127, 1131, 1180
SatI GCNGC 2 cut(s) 295, 932
Sau3AI GATC 4 cut(s) 136, 722, 1009, 1153
Sau96I GGNCC 2 cut(s) 511, 1058
SchI GAGTC 1 cut(s) 1105
ScrFI CCNGG 3 cut(s) 582, 804, 1062
SduI GDGCHC 4 cut(s) 598, 664, 803, 1087
SfaNI GCATC 2 cut(s) 65, 635
SinI GGWCC 2 cut(s) 511, 1058
SmiMI CAYNNNNRTG 3 cut(s) 308, 560, 965
SmlI CTYRAG 1 cut(s) 1113
SmoI CTYRAG 1 cut(s) 1113
Sse9I AATT 6 cut(s) 42, 298, 337, 794, 1048, 1128
SsiI CCGC 3 cut(s) 602, 648, 931
SspMI CTAG 2 cut(s) 917, 1017
SstI GAGCTC 1 cut(s) 598
StyD4I CCNGG 3 cut(s) 580, 802, 1060
StyI CCWWGG 1 cut(s) 852
TaaI ACNGT 6 cut(s) 290, 310, 511, 770, 866, 1167
TaiI ACGT 7 cut(s) 360, 428, 564, 591, 912, 946, 1139
TaqI TCGA 2 cut(s) 444, 772
TaqII GACCGA 1 cut(s) 986
TasI AATT 6 cut(s) 42, 298, 337, 794, 1048, 1128
TatI WGTACW 1 cut(s) 381
TauI GCSGC 1 cut(s) 934
TfiI GAWTC 3 cut(s) 400, 615, 629
Tru1I TTAA 6 cut(s) 72, 819, 1104, 1127, 1131, 1180
Tru9I TTAA 6 cut(s) 72, 819, 1104, 1127, 1131, 1180
TscAI CASTG 3 cut(s) 336, 478, 871
TseFI GTSAC 2 cut(s) 829, 905
TseI GCWGC 1 cut(s) 294
Tsp45I GTSAC 2 cut(s) 829, 905
TspDTI ATGAA 8 cut(s) 17, 105, 402, 544, 572, 642, 665, 724
TspGWI ACGGA 1 cut(s) 837
TspRI CASTG 3 cut(s) 336, 478, 871
Van91I CCANNNNNTGG 1 cut(s) 462
VpaK11BI GGWCC 2 cut(s) 511, 1058
VspI ATTAAT 1 cut(s) 1127
XapI RAATTY 1 cut(s) 337
XceI RCATGY 1 cut(s) 382
XmnI GAANNNNTTC 1 cut(s) 1001
XspI CTAG 2 cut(s) 917, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.