Rroxscaffold_2G00094960

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012811
Physical Location & Seq
Forward (+)
16294616 .. 16297816
3201 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00094960.1

Sequence Viewer

Length: 1203 bp
ATGACGAGGCTCAAAAGCATATCCCTAACCTTTCTTGTTGCTATTTTTGCAGTTTTATGGGCATGCAAACATCTCATAGTTTTTAATCCAAACTCAAAATACCTCAAATACTTTGAAGATCTTGGTCTCACCACCTCTTCAATATCACCTATTTTGTCTCTTCTCTTCCCATACTCCATCAACTTTAATGCTAGGCATCACAAAAAGAAAGATAATAAGGCCAAAAAAGCGTCAATTTGTGATGACTTCCCTCCTGGAATACCTCCTCCAGATACCAACACAACCTCCTATTTTTGCGTTGATCGAAATGGGTGCTGCAATTTTACTTTGGTTCAAGCAGCTGTTGATGCAGTTCCAGCTCTTAGCCAGAAAAGAACAGTATTATGGATCAATACTGGCATATACTATGAAAAAGTCATTGTTCCAATCACCAAACCAAACATAACATTCCAAGGACAAGGGTATTTATCGACTGCAATATCATGGAATGACACGGCGAATTCATCACATGGCACATTCTACAGTGGCTCTGTTCAAATTTTTGGTGCCAACTTCATTGCTAAGAACATAAGCTTCATGAATGTGGCTCCAATTCCAAAACCTGGTGATTTTGGAGCGCAAGCTGTAGCAATTAGGGTATCGGGGGACCAATCTGCATTTTGGGGTTGTGGATTCTTTGGAGCTCAAGACACCCTCCATGATGATAAGGGCCGCCATTACTTTAAGGAATGCTATATTCAGGGGTCCATTGATTTCATCTTTGGCAATGCAAGGTCATTCTATGAGAATTGCCAGTTGCTTTCGATGGCAAACCCAGTAGCCCCAGGAGCAAAGAGCATTAGCGGAGCCGTTACAGCGCATGGCAGAGCTTCAAATGAGAACACTGGATATGCATTTGTGAACTGCTTTGTTGGAGGGTCTGGAAGGGTTTGGTTAGGTCGAGCATGGAGGCCTTTCTCACGTGTGGTGTTTATCAACACAACAATGACTGACATCATAGCACCAGAAGGCTGGAATGATTTCAATGACCCAACAAGAGACCAGACTATATTTTATGGAGAATACAACTGCTCAGGTGCAGGAGCTAATTTGACCATGCGGGCATCTTATGTACAGAGACTCAATGACACTCAAGCTTCTCCTTTCTTTAATGTGTCCTTCATTGATGGAAATGATTGGTTACAACCCATTAATGCTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

400

Amino Acids

44.13

Weight (kDa)

8.59

Isoelectric Point (pI)

27.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 100 - 391 3.3e-68 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012811)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05310
fragaria_vesca FvH4_6g39490
malus_domestica MD09G1139900.v1.1 MD17G1129300.v1.1
prunus_persica Prupe.3G185700_v2.0.a1 Prupe.3G185700_v2.0.a1
pyrus_communis pycom09g06080 pycom17g12170
rosa_chinensis RchiOBHm_Chr2g0153521
rosa_laevigata RLG00000020705
rosa_multiflora Rmu_sc0003976.1_g000011
rosa_roxburghii Rroxscaffold_2G00094960
rosa_rugosa Rorug02G0438800
rosa_samantha Rh2AG502700 Rh2BG513500 Rh2CG488500 Rh2DG525200
rosa_wichuraiana Rw2G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 545
AccB7I CCANNNNNTGG 1 cut(s) 602
AciI CCGC 3 cut(s) 712, 843, 1099
AclWI GGATC 1 cut(s) 395
AcsI RAATTY 2 cut(s) 499, 537
AcvI CACGTG 1 cut(s) 962
AfaI GTAC 1 cut(s) 1113
AfiI CCNNNNNNNGG 1 cut(s) 602
AflIII ACRYGT 1 cut(s) 961
AgsI TTSAA 6 cut(s) 116, 141, 335, 536, 873, 1024
AjnI CCWGG 3 cut(s) 253, 601, 823
AluBI AGCT 8 cut(s) 341, 359, 573, 623, 683, 869, 1085, 1136
AluI AGCT 8 cut(s) 341, 359, 573, 623, 683, 869, 1085, 1136
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 4 cut(s) 131, 162, 1032, 1111
AlwI GGATC 1 cut(s) 395
AoxI GGCC 3 cut(s) 219, 709, 950
ApeKI GCWGC 2 cut(s) 315, 338
ApoI RAATTY 2 cut(s) 499, 537
AseI ATTAAT 1 cut(s) 1191
Asp700I GAANNNNTTC 1 cut(s) 1019
AspLEI GCGC 2 cut(s) 619, 859
AspS9I GGNCC 3 cut(s) 646, 709, 744
AsuHPI GGTGA 4 cut(s) 121, 138, 421, 617
AvaII GGWCC 2 cut(s) 646, 744
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 1 cut(s) 685
BbrPI CACGTG 1 cut(s) 962
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 2 cut(s) 302, 350
BccI CCATC 3 cut(s) 185, 799, 1160
BceAI ACGGC 2 cut(s) 510, 833
BcgI CGANNNNNNTGC 2 cut(s) 294, 328
BciT130I CCWGG 3 cut(s) 255, 603, 825
BcoDI GTCTC 4 cut(s) 131, 162, 1032, 1111
BfaI CTAG 2 cut(s) 192, 1201
BfmI CTRYAG 2 cut(s) 520, 624
BglII AGATCT 1 cut(s) 118
BisI GCNGC 3 cut(s) 316, 339, 712
BlsI GCNGC 3 cut(s) 317, 340, 713
Bme1390I CCNGG 3 cut(s) 255, 603, 825
Bme18I GGWCC 2 cut(s) 646, 744
BmgT120I GGNCC 3 cut(s) 646, 709, 744
BmiI GGNNCC 5 cut(s) 547, 588, 647, 745, 847
BmrFI CCNGG 3 cut(s) 255, 603, 825
BmrI ACTGGG 1 cut(s) 809
BmsI GCATC 3 cut(s) 205, 337, 1112
BmuI ACTGGG 1 cut(s) 809
BpmI CTGGAG 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 1072
BpuEI CTTGAG 2 cut(s) 669, 1116
BsaAI YACGTR 1 cut(s) 962
BsaI GGTCTC 2 cut(s) 131, 1032
BsaJI CCNNGG 2 cut(s) 451, 823
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse1I ACTGG 4 cut(s) 400, 793, 815, 889
Bse3DI GCAATG 2 cut(s) 555, 772
BseBI CCWGG 3 cut(s) 255, 603, 825
BseDI CCNNGG 2 cut(s) 451, 823
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMI GCAATG 2 cut(s) 555, 772
BseMII CTCAG 1 cut(s) 1086
BseNI ACTGG 4 cut(s) 400, 793, 815, 889
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 2 cut(s) 302, 350
BsgI GTGCAG 1 cut(s) 1098
BshFI GGCC 3 cut(s) 221, 711, 952
BshNI GGYRCC 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 685
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 1 cut(s) 602
BsmAI GTCTC 4 cut(s) 131, 162, 1032, 1111
BsmFI GGGAC 1 cut(s) 659
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 3 cut(s) 221, 711, 952
Bso31I GGTCTC 2 cut(s) 131, 1032
Bsp1286I GDGCHC 1 cut(s) 685
Bsp1407I TGTACA 1 cut(s) 1111
Bsp143I GATC 3 cut(s) 118, 301, 387
BspACI CCGC 3 cut(s) 712, 843, 1099
BspANI GGCC 3 cut(s) 221, 711, 952
BspCNI CTCAG 1 cut(s) 1085
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 5 cut(s) 547, 588, 647, 745, 847
BspPI GGATC 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 545
BspTNI GGTCTC 2 cut(s) 131, 1032
BsrDI GCAATG 2 cut(s) 555, 772
BsrGI TGTACA 1 cut(s) 1111
BsrI ACTGG 4 cut(s) 400, 793, 815, 889
BssECI CCNNGG 2 cut(s) 451, 823
BssMI GATC 3 cut(s) 118, 301, 387
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 3 cut(s) 255, 603, 825
Bst4CI ACNGT 2 cut(s) 379, 524
Bst6I CTCTTC 3 cut(s) 142, 165, 170
BstAUI TGTACA 1 cut(s) 1111
BstBAI YACGTR 1 cut(s) 962
BstC8I GCNNGC 3 cut(s) 64, 621, 1101
BstDEI CTNAG 3 cut(s) 362, 561, 1072
BstHHI GCGC 2 cut(s) 619, 859
BstKTI GATC 3 cut(s) 121, 304, 390
BstMAI GTCTC 4 cut(s) 131, 162, 1032, 1111
BstMBI GATC 3 cut(s) 118, 301, 387
BstMWI GCNNNNNNNGC 6 cut(s) 47, 227, 347, 356, 827, 854
BstNI CCWGG 3 cut(s) 255, 603, 825
BstNSI RCATGY 1 cut(s) 66
BstSCI CCNGG 3 cut(s) 253, 601, 823
BstSFI CTRYAG 2 cut(s) 520, 624
BstV1I GCAGC 2 cut(s) 302, 350
BstX2I RGATCY 1 cut(s) 118
BstXI CCANNNNNNTGG 1 cut(s) 1011
BstYI RGATCY 1 cut(s) 118
BsuRI GGCC 3 cut(s) 221, 711, 952
BtsIMutI CAGTG 2 cut(s) 529, 882
Cac8I GCNNGC 3 cut(s) 64, 621, 1101
CciI TCATGA 1 cut(s) 576
CfoI GCGC 2 cut(s) 619, 859
Cfr13I GGNCC 3 cut(s) 646, 709, 744
CseI GACGC 1 cut(s) 219
CsiI ACCWGGT 1 cut(s) 601
Csp6I GTAC 1 cut(s) 1112
CviAII CATG 8 cut(s) 63, 483, 509, 577, 698, 860, 945, 1096
CviQI GTAC 1 cut(s) 1112
DdeI CTNAG 3 cut(s) 362, 561, 1072
DpnI GATC 3 cut(s) 120, 303, 389
DpnII GATC 3 cut(s) 118, 301, 387
Eam1104I CTCTTC 3 cut(s) 142, 165, 170
EarI CTCTTC 3 cut(s) 142, 165, 170
Ecl136II GAGCTC 1 cut(s) 683
Eco130I CCWWGG 1 cut(s) 451
Eco147I AGGCCT 1 cut(s) 952
Eco24I GRGCYC 1 cut(s) 685
Eco31I GGTCTC 2 cut(s) 131, 1032
Eco47I GGWCC 2 cut(s) 646, 744
Eco53kI GAGCTC 1 cut(s) 683
Eco72I CACGTG 1 cut(s) 962
EcoICRI GAGCTC 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 3 cut(s) 253, 601, 823
EcoT14I CCWWGG 1 cut(s) 451
EcoT22I ATGCAT 1 cut(s) 895
EcoT38I GRGCYC 1 cut(s) 685
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 8 cut(s) 66, 486, 512, 580, 701, 863, 948, 1099
FaqI GGGAC 1 cut(s) 659
FatI CATG 8 cut(s) 62, 482, 508, 576, 697, 859, 944, 1095
FauI CCCGC 1 cut(s) 1092
Fnu4HI GCNGC 3 cut(s) 316, 339, 712
FriOI GRGCYC 1 cut(s) 685
Fsp4HI GCNGC 3 cut(s) 316, 339, 712
FspBI CTAG 2 cut(s) 192, 1201
GlaI GCGC 2 cut(s) 618, 858
GluI GCNGC 3 cut(s) 316, 339, 712
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 3 cut(s) 221, 711, 952
HgaI GACGC 1 cut(s) 219
HhaI GCGC 2 cut(s) 619, 859
Hin1II CATG 8 cut(s) 66, 486, 512, 580, 701, 863, 948, 1099
Hin6I GCGC 2 cut(s) 617, 857
HinP1I GCGC 2 cut(s) 617, 857
HindIII AAGCTT 2 cut(s) 571, 1134
HinfI GANTC 2 cut(s) 672, 1119
HphI GGTGA 4 cut(s) 121, 138, 421, 617
Hpy166II GTNNAC 1 cut(s) 901
Hpy188III TCNNGA 4 cut(s) 269, 577, 686, 921
Hpy8I GTNNAC 1 cut(s) 901
HpyAV CCTTC 3 cut(s) 918, 1001, 1168
HpyCH4III ACNGT 2 cut(s) 379, 524
HpyCH4IV ACGT 1 cut(s) 961
HpyCH4V TGCA 9 cut(s) 50, 66, 318, 350, 476, 656, 770, 893, 1079
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 227, 347, 356, 827, 854
HpyF3I CTNAG 3 cut(s) 362, 561, 1072
HpySE526I ACGT 1 cut(s) 961
Hsp92II CATG 8 cut(s) 66, 486, 512, 580, 701, 863, 948, 1099
HspAI GCGC 2 cut(s) 617, 857
Kzo9I GATC 3 cut(s) 118, 301, 387
LmnI GCTCC 6 cut(s) 592, 614, 680, 827, 845, 1082
Lsp1109I GCAGC 2 cut(s) 302, 350
LweI GCATC 3 cut(s) 205, 337, 1112
MabI ACCWGGT 1 cut(s) 601
MaeI CTAG 2 cut(s) 192, 1201
MaeII ACGT 1 cut(s) 961
MaeIII GTNAC 2 cut(s) 850, 1179
MalI GATC 3 cut(s) 120, 303, 389
MboI GATC 3 cut(s) 118, 301, 387
MboII GAAGA 4 cut(s) 128, 129, 152, 157
MflI RGATCY 1 cut(s) 118
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 8 cut(s) 234, 319, 499, 537, 591, 630, 787, 1087
MlyI GAGTC 1 cut(s) 1113
MmeI TCCRAC 1 cut(s) 892
MnlI CCTC 9 cut(s) 113, 145, 261, 273, 276, 295, 704, 908, 942
Mph1103I ATGCAT 1 cut(s) 895
MroXI GAANNNNTTC 1 cut(s) 1019
MseI TTAA 5 cut(s) 84, 186, 723, 1149, 1191
MslI CAYNNNNRTG 2 cut(s) 581, 983
MspA1I CMGCKG 1 cut(s) 341
MspR9I CCNGG 3 cut(s) 255, 603, 825
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 3 cut(s) 255, 603, 825
MwoI GCNNNNNNNGC 6 cut(s) 47, 227, 347, 356, 827, 854
NdeII GATC 3 cut(s) 118, 301, 387
NlaIII CATG 8 cut(s) 66, 486, 512, 580, 701, 863, 948, 1099
NlaIV GGNNCC 5 cut(s) 547, 588, 647, 745, 847
NsiI ATGCAT 1 cut(s) 895
NspI RCATGY 1 cut(s) 66
PaeI GCATGC 1 cut(s) 66
PagI TCATGA 1 cut(s) 576
PceI AGGCCT 1 cut(s) 952
PctI GAATGC 1 cut(s) 734
PdmI GAANNNNTTC 1 cut(s) 1019
PfeI GAWTC 1 cut(s) 672
PflMI CCANNNNNTGG 1 cut(s) 602
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 3 cut(s) 317, 340, 713
PleI GAGTC 1 cut(s) 1113
PmaCI CACGTG 1 cut(s) 962
PmlI CACGTG 1 cut(s) 962
PpsI GAGTC 1 cut(s) 1113
Ppu21I YACGTR 1 cut(s) 962
PshBI ATTAAT 1 cut(s) 1191
Psp124BI GAGCTC 1 cut(s) 685
Psp6I CCWGG 3 cut(s) 253, 601, 823
PspCI CACGTG 1 cut(s) 962
PspGI CCWGG 3 cut(s) 253, 601, 823
PspN4I GGNNCC 5 cut(s) 547, 588, 647, 745, 847
PspPI GGNCC 3 cut(s) 646, 709, 744
PsuI RGATCY 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 341
RsaI GTAC 1 cut(s) 1113
RsaNI GTAC 1 cut(s) 1112
RseI CAYNNNNRTG 2 cut(s) 581, 983
SacI GAGCTC 1 cut(s) 685
SaqAI TTAA 5 cut(s) 84, 186, 723, 1149, 1191
SatI GCNGC 3 cut(s) 316, 339, 712
Sau3AI GATC 3 cut(s) 118, 301, 387
Sau96I GGNCC 3 cut(s) 646, 709, 744
SchI GAGTC 1 cut(s) 1113
ScrFI CCNGG 3 cut(s) 255, 603, 825
SduI GDGCHC 1 cut(s) 685
SexAI ACCWGGT 1 cut(s) 601
SfaNI GCATC 3 cut(s) 205, 337, 1112
SfcI CTRYAG 2 cut(s) 520, 624
SinI GGWCC 2 cut(s) 646, 744
SmiMI CAYNNNNRTG 2 cut(s) 581, 983
SmlI CTYRAG 2 cut(s) 684, 1131
SmoI CTYRAG 2 cut(s) 684, 1131
SphI GCATGC 1 cut(s) 66
Sse9I AATT 8 cut(s) 234, 319, 499, 537, 591, 630, 787, 1087
SseBI AGGCCT 1 cut(s) 952
SsiI CCGC 3 cut(s) 712, 843, 1099
SspMI CTAG 2 cut(s) 192, 1201
SstI GAGCTC 1 cut(s) 685
StuI AGGCCT 1 cut(s) 952
StyD4I CCNGG 3 cut(s) 253, 601, 823
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 2 cut(s) 379, 524
TaiI ACGT 1 cut(s) 964
TaqI TCGA 4 cut(s) 304, 470, 803, 940
TasI AATT 8 cut(s) 234, 319, 499, 537, 591, 630, 787, 1087
TatI WGTACW 1 cut(s) 1111
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 1 cut(s) 672
Tru1I TTAA 5 cut(s) 84, 186, 723, 1149, 1191
Tru9I TTAA 5 cut(s) 84, 186, 723, 1149, 1191
TscAI CASTG 2 cut(s) 529, 889
TseI GCWGC 2 cut(s) 315, 338
TspDTI ATGAA 7 cut(s) 423, 492, 544, 565, 593, 745, 1150
TspRI CASTG 2 cut(s) 529, 889
Van91I CCANNNNNTGG 1 cut(s) 602
VpaK11BI GGWCC 2 cut(s) 646, 744
VspI ATTAAT 1 cut(s) 1191
XapI RAATTY 2 cut(s) 499, 537
XceI RCATGY 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 1019
XspI CTAG 2 cut(s) 192, 1201
Zsp2I ATGCAT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.