FvH4_6g39490

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012811
Physical Location & Seq
Reverse (-)
31178529 .. 31180628
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39490.t1

Sequence Viewer

Length: 1245 bp
ATGAATCCAAACATCCCAGGTTCCTCTCAACCTCTTCAGAAAATGATGAGTCTCAAAAGCATACCACTAGCCTTTCTTGTTGCCATTTTCGCAGTTTTATGGTCATGTAAACATCTCATTATCTCTAATCCAAACTCACATTACCTCAAATACTTTGAAGGTCTCGGTCTCACCACTGCTTCGATATCGCCTATTGTGTCTCTTCTCATCCCATACTCCATCAAATATAATGCTAGGCACCACAAAAAGCATGATAAAAAGACCAAAAAAGTGTCAATCTGTGATGACTTCCCTCCTGGAATACCTCCTCCAGATACCAACACAACCTCCTATTTATGCGTTGATCGGAACGGGTGCTGCAATTTCACTTTAGTTCAATCAGCTGTTGATGCAGTTCCAGCTCTTAGCCAGAAACGAACAGTAATATGGATCAACAATGGAATTTACTATGAGAAAGTCACTGTTCCAAGCGCCAAACCGAACATAACATTCCAAGGACAAGGGTATTTTTCGACTGCAATATCATGGAATGACACAGCGAATTCATCGCATGGCACATTTTATAGTGGCTCCGTTCAAGTTTTTGGTGCCAACTTCATTGCTAAGAATATAAGCTTCATGAATGTAGCTCCGATTCCAAAACCCGGTGATGTAGGAGCGCAAGCTGTGGCAATTAGGGTATCGGGGGACCAATCTGCATTTTGGGGTTGTGGATTCTTTGGAGCTCAAGATACCCTCCATGATGATAAGGGACGCCATTACTTTAGGGATTGCTATATTCAGGGTTCCATCGATTTCATCTTTGGCAATGCAAGGTCATTATATGAGAATTGCCAATTGGTTTCGATGGCAAACCCGGTAGCCCCAGGAGCAAAGATCATTAATGGAGCCCTAACAGCGCATGGCAGAGCTTCAAATGAAAACACTGGATACTCATTTGTGAACTGCATTGTTGGAGGGTCTGGAAGGGTTTGGTTAGGTCGAGCATGGAGGCCTTTCTCACGCGTGGTGTTCATCAACACAACAATGACTGACATCATAGCTCCAGAAGGCTGGAATGATTTCAATGACCCAACAAGAGACCAGACTATATTCTATGGAGAATACAATTGCTCAGGTGCAGGAGCTAATTTGACCATGCGGGCATCTTATGTACAGAAACTCAATGACACTCAAGCTTCTCCTTTCCTTAATGTGTCCTTCATTGATGGAAATGATTGGTTACAACCCATGGATGCTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

415

Amino Acids

45.4

Weight (kDa)

8.58

Isoelectric Point (pI)

28.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 114 - 405 1.9e-70 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012811)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05310
fragaria_vesca FvH4_6g39490
malus_domestica MD09G1139900.v1.1 MD17G1129300.v1.1
prunus_persica Prupe.3G185700_v2.0.a1 Prupe.3G185700_v2.0.a1
pyrus_communis pycom09g06080 pycom17g12170
rosa_chinensis RchiOBHm_Chr2g0153521
rosa_laevigata RLG00000020705
rosa_multiflora Rmu_sc0003976.1_g000011
rosa_roxburghii Rroxscaffold_2G00094960
rosa_rugosa Rorug02G0438800
rosa_samantha Rh2AG502700 Rh2BG513500 Rh2CG488500 Rh2DG525200
rosa_wichuraiana Rw2G041280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 237, 587
AccII CGCG 1 cut(s) 1005
AciI CCGC 1 cut(s) 1141
AclWI GGATC 1 cut(s) 437
AcsI RAATTY 2 cut(s) 441, 541
AcuI CTGAAG 1 cut(s) 20
AcyI GRCGYC 1 cut(s) 754
AfaI GTAC 1 cut(s) 1155
AfiI CCNNNNNNNGG 1 cut(s) 644
AflIII ACRYGT 1 cut(s) 1003
AgsI TTSAA 5 cut(s) 158, 377, 578, 915, 1066
AjnI CCWGG 3 cut(s) 16, 295, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 821, 853
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 5 cut(s) 56, 167, 173, 204, 1074
AlwI GGATC 1 cut(s) 437
AoxI GGCC 1 cut(s) 992
ApeKI GCWGC 1 cut(s) 357
ApoI RAATTY 2 cut(s) 441, 541
AseI ATTAAT 1 cut(s) 882
Asp700I GAANNNNTTC 1 cut(s) 1061
AspLEI GCGC 3 cut(s) 473, 661, 901
AspS9I GGNCC 1 cut(s) 688
AsuC2I CCSGG 2 cut(s) 645, 857
AsuHPI GGTGA 2 cut(s) 163, 659
AvaII GGWCC 1 cut(s) 688
BanI GGYRCC 2 cut(s) 237, 587
BanII GRGCYC 2 cut(s) 727, 892
Bbv12I GWGCWC 1 cut(s) 727
BbvI GCAGC 1 cut(s) 344
BccI CCATC 4 cut(s) 227, 797, 841, 1202
BciT130I CCWGG 3 cut(s) 18, 297, 867
BciVI GTATCC 1 cut(s) 923
BcnI CCSGG 2 cut(s) 645, 857
BcoDI GTCTC 5 cut(s) 56, 167, 173, 204, 1074
BfaI CTAG 3 cut(s) 68, 234, 1243
BfoI RGCGCY 1 cut(s) 474
BfuI GTATCC 1 cut(s) 923
BisI GCNGC 1 cut(s) 358
BlsI GCNGC 1 cut(s) 359
Bme1390I CCNGG 5 cut(s) 18, 297, 645, 857, 867
Bme18I GGWCC 1 cut(s) 688
BmgT120I GGNCC 1 cut(s) 688
BmiI GGNNCC 7 cut(s) 22, 239, 571, 589, 689, 787, 889
BmrFI CCNGG 5 cut(s) 18, 297, 645, 857, 867
BmsI GCATC 3 cut(s) 379, 1154, 1225
BpmI CTGGAG 2 cut(s) 294, 1029
Bpu10I CCTNAGC 1 cut(s) 1114
BpuEI CTTGAG 2 cut(s) 711, 1158
BpuMI CCSGG 2 cut(s) 645, 857
Bsa29I ATCGAT 1 cut(s) 792
BsaHI GRCGYC 1 cut(s) 754
BsaI GGTCTC 3 cut(s) 167, 173, 1074
BsaJI CCNNGG 4 cut(s) 16, 493, 865, 1230
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 1 cut(s) 644
Bse1I ACTGG 1 cut(s) 931
Bse3DI GCAATG 2 cut(s) 597, 814
BseBI CCWGG 3 cut(s) 18, 297, 867
BseCI ATCGAT 1 cut(s) 792
BseDI CCNNGG 4 cut(s) 16, 493, 865, 1230
BseGI GGATG 3 cut(s) 12, 207, 1240
BseLI CCNNNNNNNGG 1 cut(s) 644
BseMI GCAATG 2 cut(s) 597, 814
BseMII CTCAG 1 cut(s) 1128
BseNI ACTGG 1 cut(s) 931
BseRI GAGGAG 1 cut(s) 297
BseXI GCAGC 1 cut(s) 344
BsgI GTGCAG 1 cut(s) 1140
Bsh1236I CGCG 1 cut(s) 1005
BshFI GGCC 1 cut(s) 994
BshNI GGYRCC 2 cut(s) 237, 587
BshVI ATCGAT 1 cut(s) 792
BsiHKAI GWGCWC 1 cut(s) 727
BsiSI CCGG 2 cut(s) 645, 857
BslFI GGGAC 2 cut(s) 701, 765
BslI CCNNNNNNNGG 1 cut(s) 644
BsmAI GTCTC 5 cut(s) 56, 167, 173, 204, 1074
BsmFI GGGAC 2 cut(s) 701, 765
BsnI GGCC 1 cut(s) 994
Bso31I GGTCTC 3 cut(s) 167, 173, 1074
Bsp1286I GDGCHC 2 cut(s) 727, 892
Bsp1407I TGTACA 1 cut(s) 1153
Bsp143I GATC 3 cut(s) 343, 429, 876
Bsp19I CCATGG 1 cut(s) 1230
BspACI CCGC 1 cut(s) 1141
BspANI GGCC 1 cut(s) 994
BspCNI CTCAG 1 cut(s) 1127
BspDI ATCGAT 1 cut(s) 792
BspFNI CGCG 1 cut(s) 1005
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 7 cut(s) 22, 239, 571, 589, 689, 787, 889
BspPI GGATC 1 cut(s) 437
BspT107I GGYRCC 2 cut(s) 237, 587
BspTNI GGTCTC 3 cut(s) 167, 173, 1074
BsrDI GCAATG 2 cut(s) 597, 814
BsrGI TGTACA 1 cut(s) 1153
BsrI ACTGG 1 cut(s) 931
BssECI CCNNGG 4 cut(s) 16, 493, 865, 1230
BssMI GATC 3 cut(s) 343, 429, 876
BssNI GRCGYC 1 cut(s) 754
BssT1I CCWWGG 2 cut(s) 493, 1230
Bst2UI CCWGG 3 cut(s) 18, 297, 867
Bst4CI ACNGT 2 cut(s) 421, 463
Bst6I CTCTTC 2 cut(s) 39, 207
BstACI GRCGYC 1 cut(s) 754
BstAUI TGTACA 1 cut(s) 1153
BstC8I GCNNGC 2 cut(s) 663, 1143
BstDEI CTNAG 3 cut(s) 404, 603, 1114
BstDSI CCRYGG 1 cut(s) 1230
BstF5I GGATG 3 cut(s) 12, 207, 1240
BstFNI CGCG 1 cut(s) 1005
BstH2I RGCGCY 1 cut(s) 474
BstHHI GCGC 3 cut(s) 473, 661, 901
BstKTI GATC 3 cut(s) 346, 432, 879
BstMAI GTCTC 5 cut(s) 56, 167, 173, 204, 1074
BstMBI GATC 3 cut(s) 343, 429, 876
BstMWI GCNNNNNNNGC 5 cut(s) 89, 389, 398, 869, 896
BstNI CCWGG 3 cut(s) 18, 297, 867
BstSCI CCNGG 5 cut(s) 16, 295, 643, 855, 865
BstUI CGCG 1 cut(s) 1005
BstV1I GCAGC 1 cut(s) 344
BstXI CCANNNNNNTGG 1 cut(s) 1053
Bsu15I ATCGAT 1 cut(s) 792
BsuI GTATCC 1 cut(s) 923
BsuRI GGCC 1 cut(s) 994
BsuTUI ATCGAT 1 cut(s) 792
BtgI CCRYGG 1 cut(s) 1230
BtgZI GCGATG 1 cut(s) 531
BtsCI GGATG 3 cut(s) 12, 207, 1240
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 3 cut(s) 174, 459, 924
Cac8I GCNNGC 2 cut(s) 663, 1143
CciI TCATGA 1 cut(s) 618
CfoI GCGC 3 cut(s) 473, 661, 901
Cfr13I GGNCC 1 cut(s) 688
ClaI ATCGAT 1 cut(s) 792
CseI GACGC 1 cut(s) 762
Csp6I GTAC 1 cut(s) 1154
CviQI GTAC 1 cut(s) 1154
DdeI CTNAG 3 cut(s) 404, 603, 1114
DpnI GATC 3 cut(s) 345, 431, 878
DpnII GATC 3 cut(s) 343, 429, 876
Eam1104I CTCTTC 2 cut(s) 39, 207
EarI CTCTTC 2 cut(s) 39, 207
Ecl136II GAGCTC 1 cut(s) 725
Eco130I CCWWGG 2 cut(s) 493, 1230
Eco147I AGGCCT 1 cut(s) 994
Eco24I GRGCYC 2 cut(s) 727, 892
Eco31I GGTCTC 3 cut(s) 167, 173, 1074
Eco32I GATATC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 688
Eco53kI GAGCTC 1 cut(s) 725
Eco57I CTGAAG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 725
EcoRI GAATTC 1 cut(s) 541
EcoRII CCWGG 3 cut(s) 16, 295, 865
EcoRV GATATC 1 cut(s) 186
EcoT14I CCWWGG 2 cut(s) 493, 1230
EcoT38I GRGCYC 2 cut(s) 727, 892
ErhI CCWWGG 2 cut(s) 493, 1230
FaqI GGGAC 2 cut(s) 701, 765
FauI CCCGC 1 cut(s) 1134
Fnu4HI GCNGC 1 cut(s) 358
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 2 cut(s) 727, 892
Fsp4HI GCNGC 1 cut(s) 358
FspBI CTAG 3 cut(s) 68, 234, 1243
GlaI GCGC 3 cut(s) 472, 660, 900
GluI GCNGC 1 cut(s) 358
GsuI CTGGAG 2 cut(s) 294, 1029
HaeII RGCGCY 1 cut(s) 474
HaeIII GGCC 1 cut(s) 994
HapII CCGG 2 cut(s) 645, 857
HgaI GACGC 1 cut(s) 762
HhaI GCGC 3 cut(s) 473, 661, 901
Hin1I GRCGYC 1 cut(s) 754
Hin6I GCGC 3 cut(s) 471, 659, 899
HinP1I GCGC 3 cut(s) 471, 659, 899
HindIII AAGCTT 2 cut(s) 613, 1176
HinfI GANTC 4 cut(s) 4, 49, 634, 714
HpaII CCGG 2 cut(s) 645, 857
HphI GGTGA 2 cut(s) 163, 659
Hpy166II GTNNAC 2 cut(s) 110, 943
Hpy188I TCNGA 3 cut(s) 39, 348, 633
Hpy188III TCNNGA 5 cut(s) 311, 619, 728, 963, 1046
Hpy8I GTNNAC 2 cut(s) 110, 943
HpyAV CCTTC 4 cut(s) 152, 960, 1043, 1210
HpyCH4III ACNGT 2 cut(s) 421, 463
HpyCH4V TGCA 7 cut(s) 360, 392, 518, 698, 812, 948, 1121
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 389, 398, 869, 896
HpyF3I CTNAG 3 cut(s) 404, 603, 1114
Hsp92I GRCGYC 1 cut(s) 754
HspAI GCGC 3 cut(s) 471, 659, 899
Kzo9I GATC 3 cut(s) 343, 429, 876
LmnI GCTCC 8 cut(s) 575, 634, 656, 722, 869, 887, 1048, 1124
Lsp1109I GCAGC 1 cut(s) 344
LweI GCATC 3 cut(s) 379, 1154, 1225
MaeI CTAG 3 cut(s) 68, 234, 1243
MaeIII GTNAC 2 cut(s) 457, 1221
MalI GATC 3 cut(s) 345, 431, 878
MboI GATC 3 cut(s) 343, 429, 876
MboII GAAGA 2 cut(s) 26, 194
MfeI CAATTG 2 cut(s) 836, 1108
MhlI GDGCHC 2 cut(s) 727, 892
MluCI AATT 8 cut(s) 361, 441, 541, 672, 829, 836, 1108, 1129
MluI ACGCGT 1 cut(s) 1003
MlyI GAGTC 1 cut(s) 58
MmeI TCCRAC 1 cut(s) 934
MroXI GAANNNNTTC 1 cut(s) 1061
MseI TTAA 2 cut(s) 882, 1191
MslI CAYNNNNRTG 1 cut(s) 1025
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 2 cut(s) 645, 857
MspR9I CCNGG 5 cut(s) 18, 297, 645, 857, 867
MunI CAATTG 2 cut(s) 836, 1108
MvaI CCWGG 3 cut(s) 18, 297, 867
MvnI CGCG 1 cut(s) 1005
MwoI GCNNNNNNNGC 5 cut(s) 89, 389, 398, 869, 896
NciI CCSGG 2 cut(s) 645, 857
NcoI CCATGG 1 cut(s) 1230
NdeII GATC 3 cut(s) 343, 429, 876
NlaIV GGNNCC 7 cut(s) 22, 239, 571, 589, 689, 787, 889
NmuCI GTSAC 1 cut(s) 457
PagI TCATGA 1 cut(s) 618
PceI AGGCCT 1 cut(s) 994
PdmI GAANNNNTTC 1 cut(s) 1061
PfeI GAWTC 3 cut(s) 4, 634, 714
PfoI TCCNGGA 1 cut(s) 295
PkrI GCNGC 1 cut(s) 359
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 882
Psp124BI GAGCTC 1 cut(s) 727
Psp6I CCWGG 3 cut(s) 16, 295, 865
PspGI CCWGG 3 cut(s) 16, 295, 865
PspN4I GGNNCC 7 cut(s) 22, 239, 571, 589, 689, 787, 889
PspPI GGNCC 1 cut(s) 688
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 1 cut(s) 1155
RsaNI GTAC 1 cut(s) 1154
RseI CAYNNNNRTG 1 cut(s) 1025
SacI GAGCTC 1 cut(s) 727
SaqAI TTAA 2 cut(s) 882, 1191
SatI GCNGC 1 cut(s) 358
Sau3AI GATC 3 cut(s) 343, 429, 876
Sau96I GGNCC 1 cut(s) 688
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 5 cut(s) 18, 297, 645, 857, 867
SduI GDGCHC 2 cut(s) 727, 892
SfaNI GCATC 3 cut(s) 379, 1154, 1225
SinI GGWCC 1 cut(s) 688
SmiMI CAYNNNNRTG 1 cut(s) 1025
SmlI CTYRAG 2 cut(s) 726, 1173
SmoI CTYRAG 2 cut(s) 726, 1173
Sse9I AATT 8 cut(s) 361, 441, 541, 672, 829, 836, 1108, 1129
SseBI AGGCCT 1 cut(s) 994
SsiI CCGC 1 cut(s) 1141
SspMI CTAG 3 cut(s) 68, 234, 1243
SstI GAGCTC 1 cut(s) 727
StuI AGGCCT 1 cut(s) 994
StyD4I CCNGG 5 cut(s) 16, 295, 643, 855, 865
StyI CCWWGG 2 cut(s) 493, 1230
TaaI ACNGT 2 cut(s) 421, 463
TaqI TCGA 5 cut(s) 182, 512, 792, 845, 982
TaqII GACCGA 1 cut(s) 155
TasI AATT 8 cut(s) 361, 441, 541, 672, 829, 836, 1108, 1129
TatI WGTACW 1 cut(s) 1153
TfiI GAWTC 3 cut(s) 4, 634, 714
Tru1I TTAA 2 cut(s) 882, 1191
Tru9I TTAA 2 cut(s) 882, 1191
TscAI CASTG 3 cut(s) 181, 466, 931
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 1 cut(s) 357
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 9 cut(s) 17, 534, 586, 607, 635, 787, 933, 1003, 1192
TspGWI ACGGA 1 cut(s) 562
TspRI CASTG 3 cut(s) 181, 466, 931
VpaK11BI GGWCC 1 cut(s) 688
VspI ATTAAT 1 cut(s) 882
XapI RAATTY 2 cut(s) 441, 541
XmnI GAANNNNTTC 1 cut(s) 1061
XspI CTAG 3 cut(s) 68, 234, 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.